CN106071404A - Multiple elements design crucian carp feeds based on Folium Mori and preparation method thereof - Google Patents

Abstract

The invention discloses multiple elements design crucian carp feed based on Folium Mori and preparation method thereof, it comprises 3 12 parts of Folium et Ramulus Mori mixture, soybean meal 20 30 parts, rapeseed meal 18 24 parts, 15 25 parts of flour, peru fish meal 12 16 parts, cottonseed meal 6 10 parts, yellow bentonite 15 parts, soybean oil 15 parts, dalcium biphosphate 13 parts, 1% fish compound premix 0.5 2 parts, 0.1 0.3 parts of choline, antioxidant 0.01 0.05 parts, lysine 0.01 0.03 parts and methionine 0.01 0.03 parts, process through Folium et Ramulus Mori mixture, mix and blend, pelletize, the step being dried prepares.The present invention with the addition of feedstuff Mulberry in feedstuff, optimizes crucian carp feed nutrition, improves fodder production process simultaneously, and palatability is preferable, additionally reduces feed cost, the production performance that improve Carassius auratus, the meat quality adding Carassius auratus and resistance against diseases.

Description

Multiple elements design crucian carp feeds based on Folium Mori and preparation method thereof

Technical field

The present invention relates to field of feed used for aquiculture, be based especially on Folium Mori multiple elements design crucian carp feed and Preparation method.

Background technology

Carassius auratus (formal name used at school: Carassius auratus, be called for short crucian carp, popular name crucian carp, moon crucian carp crucian carp young, native, thin head, shad, Cold crucian carp) it is common fresh-water fishes in In Eurasia, the one of which Fish belonged to for Actinopterygii Cypriniformes Cyprinidae crucian carp.Carassius auratus meat Delicacy, meat flavour is sweet, is of high nutritive value, and every hectogram meat is containing 13 grams of protein, 11 grams of fat, and contains substantial amounts of calcium, phosphorus, ferrum etc. Mineral.Carassius auratus medical value is high, and its nature and flavor are sweet, flat, warm, enters stomach, kidney, have with middle qi-restoratives, eliminating dampness and promoting diuresis, qi-restoratives win, Stomach warming feed, effect of invigorating middle warmer anger.Carassius auratus is widely distributed, and the most all there is production in waters in all parts of the country, important edible for China One of Fish.

The cultivation of highdensity Carassius auratus man-made feeds has become as existing major way, but high density crucian cultivation is easily led Cause some Carassius auratuss and viral or bacterial disease occurs, for solving this problem, generally from cleaning up the pond or breeding density can be controlled Etc. controlling, it is also possible to the anti-virus ability strengthening Carassius auratus from feedstuff reaches this effect.At present, crucian carp feed kind is relatively Many, but the nutrition in major part crucian carp feed dispensing is not quite reasonable, on the high side, various albumen and the content of vitamin Ratio is relatively low, and the palatability of feedstuff is poor simultaneously, and the food-intake of Carassius auratus is relatively low, and this is the most unfavorable to Carassius auratus growth, and does not temporarily have Occur a kind of feedstuff strengthening Carassius auratus anti-virus ability.

Summary of the invention

It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of nutrition is reasonable, the base of good palatability Multiple elements design crucian carp feed in Folium Mori and preparation method thereof, reduces feed cost, improves the production performance of Carassius auratus, increases Carassius auratus Meat quality and resistance against diseases.

It is an object of the invention to be achieved through the following technical solutions: a kind of multiple elements design Carassius auratuss based on Folium Mori are raised Material, it comprises the raw material of following weight portion: Folium et Ramulus Mori mixture 3-12 part, soybean meal 20-30 part, rapeseed meal 18-24 part, flour 15-25 part, peru fish meal 12-16 part, cottonseed meal 6-10 part, yellow bentonite 1-5 part, soybean oil 1-5 part, dalcium biphosphate 1- 3 parts, 1% fish compound premix 0.5-2 part, choline 0.1-0.3 part, antioxidant 0.01-0.05 part, lysine 0.01- 0.03 part and methionine 0.01-0.03 part, wherein, in described Folium et Ramulus Mori mixture, Ramulus Mori and Folium Mori are according to 1:1-1.5's Weight ratio carries out proportioning.

The bentonitic granular size of described yellow is 200-400 mesh.

Described antioxidant is 30% ethoxy quinoline.

The concentration of described lysine is 90%-99%.

The manufacture method of multiple elements design crucian carp feeds based on Folium Mori, it comprises the steps of

S1, the process of Folium et Ramulus Mori mixture, comprise below step:

S101, the preparation of Mori folium extract: cleaned by the Folium Mori of above-mentioned weight portion, be subsequently adding Folium Mori weight 10-15 times Water, and be heated to 80-100 DEG C, leach 2-3 time, each 45-80min, obtain green Mori folium extract, then by extracting solution Filter or centrifugal segregation insoluble matter, set aside for use under room temperature；

S102, Ramulus Mori are pulverized: are cleaned by the Ramulus Mori of above-mentioned weight portion and dry, are ground into the fine particle of 200-400 mesh, go Except the most heterochromatic granule, set aside for use under room temperature；

S2, mix and blend: by size-reduced to the soybean meal of above-mentioned weight portion, rapeseed meal, flour, cottonseed meal, yellow bentonite Cross 60 mesh sieves, be subsequently adding Ramulus Mori fine particle, peru fish meal, soybean oil, dalcium biphosphate, 1% fish compound premix, gallbladder Alkali, antioxidant, lysine and methionine, add the water stirring 60-120min of above-mentioned weight portion 2-5 times, then add Mori folium extract stirring 15-30min, set aside for use under room temperature；

S3, granulation: mixture step S2 obtained is put in granulator, make the pellet of particle diameter 0.5-2.0mm；

S4, it is dried: above-mentioned pellet is dried at 40-45 DEG C to moisture less than 10%, be then saved in In the environment of 15-25 DEG C.

Feedstuff Mulberry is that China scientific research personnel, by artificial selection and cross-breeding, cultivates up-to-date resistance product in recent years System, is the preferable novel kind of benefit in China's Mulberry industry, and Regional suitability is strong, has the potential ecological value and feedstuff valency Value, DEVELOPMENT PROSPECT is the most wide.The feedstuff Mulberry Folium Mori that are of high nutritive value contain abundant carbohydrate, protein, fatty acid, fibre Dimension element, and vitamin and mineral element.Wherein amino acid classes is complete, reaches 18 kinds, account for the 10% of Folium Mori dry with On.In these aminoacid, animal must and non essential amino acid account for more than half of total amount, especially lysine, egg ammonia Acid, cystine, content of glutamic acid are higher, have higher nutritive value as feedstuff.From the point of view of vitamin contained from Folium Mori, often Containing Vc30-40mg, VB10.5-0.8mg, VB20.8-1.5mg, VE30-40mg, VB110.5-0.6mg, VB53-in 100g Folium Mori 5mg.The mineral nutrition of Folium Mori the most relatively horn of plenty, Folium Mori mainly contain 8 kinds of mineral elements, wherein potassium, calcium, ferrum, zinc, manganese according to surveying and determination Content is significantly higher compared with Semen Maydis, Herba Medicaginis (dark green), illustrates that Folium Mori have important meaning as feedstuff to the growth promoter of animal Justice.It addition, research discovery Folium Mori are in addition to containing abundant mineral and vitamin, possibly together with the natural activity of many special efficacies Material composition.100g dry (higher 3-4 times than green tea) Han sterol 46mg；Possibly together with isoquercitrin 200-500mg, Quercitroside 30mg, quercin 100mg etc. can suppress the Flavonoid substances of lipid oxidation effect；Containing several amino acids neurotransmission thing Matter, material for lowering blood pressure gamma-amino west acid (average content 226mg/100g)；It is named as 1-DNJ (DNJ) containing one Alkaloid, plant has only in Folium Mori and contains, it is the inhibitor of a kind of glycosidase.Poultry are had by these active substances exempts from Epidemic disease health-care effect, it is possible to prevent the generation of bird flu, improves the resistance against diseases of poultry, beneficially poultry and keeps fit the most raw Long.

Feedstuff Mulberry good palatability, digestibility height feedstuff Folium Mori, bar are used as feedstuff, and its digestibility is typically left 80% The right side, up to 95%, and there is good palatability, owing to feedstuff Mulberry is without Drug resistance, and disease-resistant factor in Carassius auratus body can be excited, Do not cause pathogenic bacteria to make a variation, its metabolism can be strengthened, promote protein and the synthesis of enzyme and body growth, containing non-specific anti- Bacterium composition, improves body immunity, antibacterial ability etc., and therefore feedstuff Mulberry is crucian carp feed effective albumen source.

In order to further illustrate the using value of the present invention, applicant chooses Carassius auratus 500 tail of about 50 ± 5g, if five Group, often organizes 100 tails, throw something and feed respectively feedstuff Mulberry content 3%, 6%, 9%, 12% and the existing feedstuff without feedstuff Mulberry, respectively Numbering 1-5 experimental group, raises 90d, and the main nutrient composition result of the test of Carassius auratus is as follows:

Sequence number	Nutrient title	1 group	2 groups	3 groups	4 groups	5 groups
							1	Dry matter content %	88.4	88.3	88.3	88.3	72.1
2	Crude protein %	34.5	34.4	34.4	34.3	22.6
							3	Calcium %	1.12	1.11	0.95	0.79	0.72
4	Total phosphorus %	1.41	1.42	1.2	0.99	1.41
							5	Available phosphorus %	1.04	1.05	0.83	0.62	0.76
6	Salt %	0.4	0.4	0.4	0.41	0.65
							7	Lysine %	1.94	1.94	1.94	1.94	1.72
8	Methionine %	0.646	0.646	0.646	0.646	0.562
							9	Egg+cystine %	1.178	1.178	1.178	1.178	0.92
10	Crude fibre %	5.3	5.5	5.7	5.9	6.7
							11	Crude fat %	5.3	5.3	5.3	5.4	7.2
12	Coarse ash %	11.5	10.6	9.9	9.1	12.3
							13	Nitrogen-free extract %	32.82	33.68	34.54	35.41	36.72
14	Magnesium %	0.23	0.23	0.23	0.23	0.21

The invention has the beneficial effects as follows: with the addition of feedstuff Mulberry in feedstuff, the feedstuff Mulberry Folium Mori that are of high nutritive value contain abundant Carbohydrate, protein, fatty acid, cellulose, and vitamin and mineral element, it is provided that crucian carp feed albumen comes Source, optimizes crucian carp feed nutrition, improves fodder production process simultaneously, and it has higher added value, and palatability is relatively Good, additionally reduce feed cost, the production performance that improve Carassius auratus, the meat quality adding Carassius auratus and resistance against diseases.

Detailed description of the invention

Below in conjunction with embodiment, technical scheme is described in further detail, but protection scope of the present invention is not limited to In the following stated.

Embodiment 1

A kind of multiple elements design crucian carp feed based on Folium Mori, it comprises the raw material of following weight portion: Folium et Ramulus Mori mixture 3 Part, soybean meal 25.3 parts, rapeseed meal 22 parts, 20 parts of flour, peru fish meal 14 parts, cottonseed meal 8 parts, yellow bentonite 2 parts, Semen sojae atricolor Oil 2.5 parts, dalcium biphosphate 2 parts, 1% fish compound premix 1 part, 0.15 part of choline, antioxidant 0.02 part, lysine 0.01 part and methionine 0.01 part, wherein, in described Folium et Ramulus Mori mixture, Ramulus Mori and Folium Mori are carried out according to the weight ratio of 1:1 Proportioning.

The manufacture method of multiple elements design crucian carp feeds based on Folium Mori, it comprises the steps of

S1, the process of Folium et Ramulus Mori mixture, comprise below step:

S101, the preparation of Mori folium extract: cleaned by the Folium Mori of above-mentioned weight portion, be subsequently adding Folium Mori weight 10 times Water, and it is heated to 80 DEG C, leach 2 times, each 50min, obtain green Mori folium extract, then extracting solution is filtered, under room temperature Set aside for use；

S102, Ramulus Mori are pulverized: are cleaned by the Ramulus Mori of above-mentioned weight portion and dry, are ground into the fine particle of 200 mesh, remove it In heterochromatic granule, set aside for use under room temperature；

S2, mix and blend: by size-reduced to the soybean meal of above-mentioned weight portion, rapeseed meal, flour, cottonseed meal, yellow bentonite Cross 60 mesh sieves, be subsequently adding Ramulus Mori fine particle, peru fish meal, soybean oil, dalcium biphosphate, 1% fish compound premix, gallbladder Alkali, antioxidant, lysine and methionine, add the water stirring 100min of above-mentioned weight portion 3 times, then add Folium Mori and carry Take liquid stirring 20min, set aside for use under room temperature；

S3, granulation: mixture step S2 obtained is put in granulator, make the pellet of particle diameter 1.0mm；

S4, be dried: above-mentioned pellet is dried at 40-45 DEG C and is down to 10% to moisture, be then saved in 20 DEG C environment in.

Embodiment 2

A kind of multiple elements design crucian carp feed based on Folium Mori, it comprises the raw material of following weight portion: Folium et Ramulus Mori mixture 6 Part, soybean meal 20 parts, rapeseed meal 24 parts, 15 parts of flour, peru fish meal 12 parts, cottonseed meal 6 parts, yellow bentonite 5 parts, soybean oil 1 part, dalcium biphosphate 3 parts, 1% fish compound premix 0.5 part, 0.3 part of choline, antioxidant 0.05 part, lysine 0.03 Part and methionine 0.03 part, wherein, in described Folium et Ramulus Mori mixture, Ramulus Mori and Folium Mori are joined according to the weight ratio of 1:1.5 Ratio.

The manufacture method of multiple elements design crucian carp feeds based on Folium Mori, it comprises the steps of

S1, the process of Folium et Ramulus Mori mixture, comprise below step:

S101, the preparation of Mori folium extract: cleaned by the Folium Mori of above-mentioned weight portion, be subsequently adding Folium Mori weight 13 times Water, and it is heated to 85 DEG C, leach 3 times, each 80min, obtain green Mori folium extract, then by insoluble for extracting solution centrifugal segregation Thing, set aside for use under room temperature；

S102, Ramulus Mori are pulverized: are cleaned by the Ramulus Mori of above-mentioned weight portion and dry, are ground into the fine particle of 300 mesh, remove it In heterochromatic granule, set aside for use under room temperature；

S2, mix and blend: by size-reduced to the soybean meal of above-mentioned weight portion, rapeseed meal, flour, cottonseed meal, yellow bentonite Cross 60 mesh sieves, be subsequently adding Ramulus Mori fine particle, peru fish meal, soybean oil, dalcium biphosphate, 1% fish compound premix, gallbladder Alkali, antioxidant, lysine and methionine, add the water stirring 120min of above-mentioned weight portion 2 times, then add Folium Mori and carry Take liquid stirring 25min, set aside for use under room temperature；

S3, granulation: mixture step S2 obtained is put in granulator, make the pellet of particle diameter 0.5mm；

S4, be dried: above-mentioned pellet is dried at 40-45 DEG C and is down to 8% to moisture, be then saved in 15 DEG C Environment in.

Embodiment 3

A kind of multiple elements design crucian carp feed based on Folium Mori, it comprises the raw material of following weight portion: Folium et Ramulus Mori mixture 9 Part, soybean meal 30 parts, rapeseed meal 18 parts, 25 parts of flour, peru fish meal 16 parts, cottonseed meal 10 parts, yellow bentonite 1 part, Semen sojae atricolor Oil 5 parts, dalcium biphosphate 1 part, 1% fish compound premix 2 parts, 0.1 part of choline, antioxidant 0.01 part, lysine 0.02 Part and methionine 0.02 part, wherein, in described Folium et Ramulus Mori mixture, Ramulus Mori and Folium Mori are joined according to the weight ratio of 1:1.2 Ratio.

The manufacture method of multiple elements design crucian carp feeds based on Folium Mori, it comprises the steps of

S1, the process of Folium et Ramulus Mori mixture, comprise below step:

S101, the preparation of Mori folium extract: cleaned by the Folium Mori of above-mentioned weight portion, be subsequently adding Folium Mori weight 14 times Water, and it is heated to 85 DEG C, leach 2 times, each 60min, obtain green Mori folium extract, then by insoluble for extracting solution centrifugal segregation Thing, set aside for use under room temperature；

S102, Ramulus Mori are pulverized: are cleaned by the Ramulus Mori of above-mentioned weight portion and dry, are ground into the fine particle of 400 mesh, remove it In heterochromatic granule, set aside for use under room temperature；

S2, mix and blend: by size-reduced to the soybean meal of above-mentioned weight portion, rapeseed meal, flour, cottonseed meal, yellow bentonite Cross 60 mesh sieves, be subsequently adding Ramulus Mori fine particle, peru fish meal, soybean oil, dalcium biphosphate, 1% fish compound premix, gallbladder Alkali, antioxidant, lysine and methionine, add the water stirring 60min of above-mentioned weight portion 5 times, then add Folium Mori and carry Take liquid stirring 30min, set aside for use under room temperature；

S3, granulation: mixture step S2 obtained is put in granulator, make the pellet of particle diameter 2.0mm；

S4, be dried: above-mentioned pellet is dried at 40-45 DEG C and is down to 9% to moisture, be then saved in 25 DEG C Environment in.

Embodiment 4

A kind of multiple elements design crucian carp feed based on Folium Mori, it comprises the raw material of following weight portion: Folium et Ramulus Mori mixture 5 Part, soybean meal 22 parts, rapeseed meal 20 parts, 18 parts of flour, peru fish meal 15 parts, cottonseed meal 7 parts, yellow bentonite 4 parts, soybean oil 3 parts, dalcium biphosphate 1.5 parts, 1% fish compound premix 1.5 parts, 0.2 part of choline, antioxidant 0.04 part, lysine 0.01 part and methionine 0.01 part, wherein, in described Folium et Ramulus Mori mixture, Ramulus Mori and Folium Mori enter according to the weight ratio of 1:1.4 Row proportioning.

The manufacture method of multiple elements design crucian carp feeds based on Folium Mori, it comprises the steps of

S1, the process of Folium et Ramulus Mori mixture, comprise below step:

S101, the preparation of Mori folium extract: cleaned by the Folium Mori of above-mentioned weight portion, be subsequently adding Folium Mori weight 12 times Water, and it is heated to 85 DEG C, leach 2 times, each 60min, obtain green Mori folium extract, then by insoluble for extracting solution centrifugal segregation Thing, set aside for use under room temperature；

S102, Ramulus Mori are pulverized: are cleaned by the Ramulus Mori of above-mentioned weight portion and dry, are ground into the fine particle of 250 mesh, remove it In heterochromatic granule, set aside for use under room temperature；

S2, mix and blend: by size-reduced to the soybean meal of above-mentioned weight portion, rapeseed meal, flour, cottonseed meal, yellow bentonite Cross 60 mesh sieves, be subsequently adding Ramulus Mori fine particle, peru fish meal, soybean oil, dalcium biphosphate, 1% fish compound premix, gallbladder Alkali, antioxidant, lysine and methionine, add the water stirring 75min of above-mentioned weight portion 4 times, then add Folium Mori and carry Take liquid stirring 15min, set aside for use under room temperature；

S3, granulation: mixture step S2 obtained is put in granulator, make the pellet of particle diameter 1.5mm；

S4, be dried: above-mentioned pellet is dried at 40-45 DEG C and is down to 5% to moisture, be then saved in 20 DEG C Environment in.

Embodiment 5

A kind of multiple elements design crucian carp feed based on Folium Mori, it comprises the raw material of following weight portion: Folium et Ramulus Mori mixture 12 Part, soybean meal 28 parts, rapeseed meal 22 parts, 20 parts of flour, peru fish meal 16 parts, cottonseed meal 9 parts, yellow bentonite 3 parts, soybean oil 3 parts, dalcium biphosphate 2 parts, 1% fish compound premix 1 part, 0.3 part of choline, antioxidant 0.02 part, lysine 0.03 part And methionine 0.03 part, wherein, in described Folium et Ramulus Mori mixture, Ramulus Mori and Folium Mori are joined according to the weight ratio of 1:1.3 Ratio.

The manufacture method of multiple elements design crucian carp feeds based on Folium Mori, it comprises the steps of

S1, the process of Folium et Ramulus Mori mixture, comprise below step:

S101, the preparation of Mori folium extract: cleaned by the Folium Mori of above-mentioned weight portion, be subsequently adding Folium Mori weight 15 times Water, and it is heated to 100 DEG C, leach 3 times, each 45min, obtain green Mori folium extract, then extracting solution is filtered, under room temperature Set aside for use；

S102, Ramulus Mori are pulverized: are cleaned by the Ramulus Mori of above-mentioned weight portion and dry, are ground into the fine particle of 350 mesh, remove it In heterochromatic granule, set aside for use under room temperature；

S2, mix and blend: by size-reduced to the soybean meal of above-mentioned weight portion, rapeseed meal, flour, cottonseed meal, yellow bentonite Cross 60 mesh sieves, be subsequently adding Ramulus Mori fine particle, peru fish meal, soybean oil, dalcium biphosphate, 1% fish compound premix, gallbladder Alkali, antioxidant, lysine and methionine, add the water stirring 85min of above-mentioned weight portion 3 times, then add Folium Mori and carry Take liquid stirring 20min, set aside for use under room temperature；

S3, granulation: mixture step S2 obtained is put in granulator, make the pellet of particle diameter 1.0mm；

S4, be dried: above-mentioned pellet is dried at 40-45 DEG C and is down to 6% to moisture, be then saved in 25 DEG C Environment in.

When carrying out man-made feeds nursing Carassius auratus, all fishes descend the pool to raise after buying back after 3% saline solution sterilization 15-20min again Support, every day early, middle and late (respectively point in the morning eight, noon 12 point, afternoon six point) three feedstuffs of respectively throwing something and feeding, day feeding rate 5% left The right side, is advisable to have eaten in 20min.Patrol the pool every day to carry out and anti-escape the work of anti-harmful animal.

In order to further illustrate the using value of the present invention, inventor has carried out Carassius auratus group experiment, chooses without feedstuff The feedstuff of Sang Chengfen carries out contrast test, during test, should proceed by formal test, often organize throwing during formal test after supporting 15 days temporarily Feeding embodiment 1-5 formula forage and the contrast groups feedstuff without feedstuff Mulberry composition, respectively at throwing something and feeding, feedstuff is adopted for 30,60,90 days Sample, takes muscular tissue and tail vein blood, carries out growth test mensuration, muscle nutrition index determining and blood parameters and surveys Fixed, concrete Testing index and method are as follows:

1. growth indexes: fish body hunger 24h before weighing, weighs, and records the throwing amount of raising.Often group randomly selects 4 tail fishes, measures it Body length, body weight, internal organs weight, liver weight, intestinal long pointer, be used for calculating weight gain rate, feed coefficient, protein efficiency ratio, PER, specific Rate of growth, coefficient of condition, dirty body ratio, liver body ratio, intestinal body ratio.Computing formula is as follows:

Rate of body weight gain (%)=(fish body end weight-fish body initial weight)/fish body initial weight × 100；

Specific growth rate (Specificgrowth ratio, SGR, %/d)=(LnWt LnW0) × 100/t；

Protein efficiency ratio, PER (Protein efficiency ratio, PER, %)=(Wt W0) × 100/F × P；

Feed coefficient (the %)=throwing amount of raising/weight gain amount (all conversions are dry) × 100；

Coefficient of condition K (%)=(W/L³)×100.In formula, W is body weight (g), and L is body length (cm)；

Dirty body ratio (%)=internal organs gross weight (g)/body weight (g) × 100；

Liver body ratio (%)=liver gross weight (g)/body weight (g) × 100；

Intestinal body ratio=intestinal is long/and body is long × and 100%；

2. meat quality index (test outsourcing detection):

Protein, cholesterol, aminoacid, fatty acid, calcium, zinc, selenium, VitAVitE, ash in detection muscle.

Each experimental group chooses fish 3 tail, and every tail fish body side the 3rd to the 6th helps the about 5g of the abdominal muscle between bone, surveys muscle dehydration Rate；Take opposite side the 3rd dorsal fin to muscle about 5g, the muscle about 2g between the 6th dorsal fin to the 8th dorsal fin between the 6th dorsal fin, survey flesh Meat protein, aminoacid, fatty acid, minerals and vitamins.Each test tank chooses the frost of fish three tail live body, becomes as routine Analyze and use.

Determined amino acid HetachL-8800 automatic amino acid analyzer.The saponification of oils and fats and first in determination of fatty acid Esterification measures with reference to the method for GB/T17376-2008 and GB/T17377-2008.In Carassius auratus conventional ingredient, moisture uses 105 DEG C Oven drying method (GB6435-86)；Crude protein uses Kjeldahl's method (GB/T6432-94)；Crude fat uses ether extraction (GB/ T6433-124)；Ash uses 550 DEG C of calcination methods (GB/T6438-92).

3. physiochemical indice:

Detection triglyceride (TG), T-CHOL (TC), glutamic oxaloacetic transaminase, GOT (AST), glutamate pyruvate transaminase (ALT), total protein (TP), superoxide dismutase (T-SOD), lysozyme (LSZ), Complement C_3.Superoxide dismutase (SOD) is that one comes from life The active substance of life body, can eliminate the harmful substance that organism produces in metabolic processes, is naturally occurring in body Superoxide radical clearing factor, plays particularly important effect, in immune system also in the self-protection system of organism There is particularly important effect；Lysozyme is a kind of to hydrolyze the alkaline enzyme of mucopolysaccharide in pathogenic bacterium, mainly makes cell wall insoluble Mucopolysaccharide resolves into solubility glycopeptide, causes cell wall rupture content to escape, so that bacterolysis, lysozyme also can be with band The virus protein of negative charge directly in conjunction with, with DNA, RNA, apoprotein formed double salt, make virally inactivated, this enzyme has anti- Bacterium, antiinflammatory, antiviral etc. act on.Biochemical Indices In Serum is relevant with living environment and nutrition, and detection triglyceride (TG), total gallbladder are admittedly Alcohol (TC), glutamic oxaloacetic transaminase, GOT (AST), glutamate pyruvate transaminase (ALT) and total protein (TP) are all that body resists disease and reflection organ The important immune factor of function；Complement be present in normal person and animal serum activated with a group in tissue fluid after there is enzyme The protein of activity, can assist and supplement specific antibody, mediated immunity bacteriolyze, haemolysis.Complement C_3 is content in serum The highest complement component, molecular weight is 195000, is mainly synthesized, on complement Classical pathway and side by macrophage and liver Road activated pathway all plays a significant role.

Method: during testing inspection point, the asepsis injector of the random 3 tail Carassius auratus 5.0mL in each pond is from tail vein blood In 5.0mL sterile centrifugation tube, 2mL is used for preparing serum, and after 4 DEG C stand overnight, 4000r/min is centrifuged l0min, takes upper serum It is placed in-80 DEG C of ultra cold storage freezers to save backup.Detection TG (TG), T-CHOL (TC), glutamic oxaloacetic transaminase, GOT (AST), paddy Pyruvic transaminase (ALT), total protein (TP), superoxide dismutase (T-SOD), lysozyme (LSZ) use Nanjing to build up biological work The kit measurement of journey institute.Complement C_3 uses Zhejiang Province Yilikang Bioisystech Co., Ltd's kit measurement.

4. premunition index of correlation measures:

Detection index and spleen index and head kidney index, quantity of leucocyte and survival rate.The immune organ of animal is aquatic animal row Make the vital tissue of immunologic function.Immuning tissue is immunocyte generation, differentiation, maturation, settles down and breed and produce immunity The place of response.The weight of immune organ is closely related with Immune Function In Animals, and their development condition directly affects body Immunity.Spleen is the predominant peripheral lymphatic organ of Fish, is also the organ filtering blood, is that T, bone-marrow-derived lymphocyte are migrated and connect By the main place producing immunne response after antigenic stimulus.Therefore, the weight of spleen can reflect Fish to a certain extent Immunne response level.Head-kidney is the important immune organs of Fish.

Method: the fish having adopted blood is weighed up body weight, with dissecting tools such as operating scissorss successively sit in the right seat collection spleen and Head-kidney.By the spleen of taking-up and head-kidney with clean absorbent cotton quickly, dry gently, claim its weight, and record data.Measure spleen Index and head kidney index, including:

Index and spleen index=spleen weight/fish body weight × 10^-3；

Head kidney index=head-kidney weight/fish body weight × 10^-3。

Challenge test: counteracting toxic substances strain is Aeromonas hydrophila (Aeromonas hydrophila).By Aeromonas hydrophila With 1% normal saline eluting lawn, accurately allocate with spectrophotometer, make final concentration of 1.0 × 10⁶CFU/mL.All tests The 10 tail fishes that respectively take of group carry out counteracting toxic substances, every tail lumbar injection 0.1mL；Matched group injection same dose normal saline is as counteracting toxic substances pair According to, observe 1 week, add up each group of survival rate.

By Experimental Comparison, the Carassius auratus of the diet of the present invention is used to refer at growth indexes, meat quality index, blood It is marked with and has had in resistance against diseases and be obviously improved.

The above is only the preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein Form, is not to be taken as the eliminating to other embodiments, and can be used for other combinations various, amendment and environment, and can be at this In the described contemplated scope of literary composition, it is modified by above-mentioned teaching or the technology of association area or knowledge.And those skilled in the art are entered The change of row and change, the most all should be at the protection domains of claims of the present invention without departing from the spirit and scope of the present invention In.

Claims (5)

1. a multiple elements design crucian carp feed based on Folium Mori, it is characterised in that it comprises the raw material of following weight portion: Folium et Ramulus Mori Mixture 3-12 part, soybean meal 20-30 part, rapeseed meal 18-24 part, flour 15-25 part, peru fish meal 12-16 part, cottonseed meal 6- 10 parts, yellow bentonite 1-5 part, soybean oil 1-5 part, dalcium biphosphate 1-3 part, 1% fish compound premix 0.5-2 part, choline 0.1-0.3 part, antioxidant 0.01-0.05 part, lysine 0.01-0.03 part and methionine 0.01-0.03 part, wherein, institute In the Folium et Ramulus Mori mixture stated, Ramulus Mori and Folium Mori carry out proportioning according to the weight ratio of 1:1-1.5.

2. multiple elements design crucian carp feeds based on Folium Mori as claimed in claim 1, it is characterised in that: described yellow bentonite Granular size be 200-400 mesh.

3. multiple elements design crucian carp feeds based on Folium Mori as claimed in claim 1, it is characterised in that: described antioxidant is 30% ethoxy quinoline.

4. multiple elements design crucian carp feeds based on Folium Mori as claimed in claim 1, it is characterised in that: described lysine dense Degree is 90%-99%.

5. the manufacture method of multiple elements design crucian carp feeds based on Folium Mori as claimed in claim 1, it is characterised in that it comprises Following steps:

S1, the process of Folium et Ramulus Mori mixture, comprise below step:

S101, the preparation of Mori folium extract: cleaned by the Folium Mori of above-mentioned weight portion, be subsequently adding the water of Folium Mori weight 10-15 times, And be heated to 80-100 DEG C, leach 2-3 time, each 45-80min, obtain green Mori folium extract, then extracting solution is filtered or Centrifugal segregation insoluble matter, set aside for use under room temperature；

S102, Ramulus Mori are pulverized: are cleaned by the Ramulus Mori of above-mentioned weight portion and dry, are ground into the fine particle of 200-400 mesh, remove it In heterochromatic granule, set aside for use under room temperature；

S2, mix and blend: by the soybean meal of above-mentioned weight portion, rapeseed meal, flour, cottonseed meal, the size-reduced mistake of yellow bentonite 60 Mesh sieve, is subsequently adding Ramulus Mori fine particle, peru fish meal, soybean oil, dalcium biphosphate, 1% fish compound premix, choline, resists Oxidant, lysine and methionine, add the water stirring 60-120min of above-mentioned weight portion 2-5 times, then add Folium Mori and carry Take liquid stirring 15-30min, set aside for use under room temperature；

S3, granulation: mixture step S2 obtained is put in granulator, make the pellet of particle diameter 0.5-2.0mm；

S4, it is dried: above-mentioned pellet is dried at 40-45 DEG C to moisture less than 10%, be then saved in 15- In the environment of 25 DEG C.