CN108835384A - A kind of prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement - Google Patents
A kind of prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement Download PDFInfo
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- CN108835384A CN108835384A CN201810867159.1A CN201810867159A CN108835384A CN 108835384 A CN108835384 A CN 108835384A CN 201810867159 A CN201810867159 A CN 201810867159A CN 108835384 A CN108835384 A CN 108835384A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
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Abstract
A kind of prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement has the characteristics that enhance immunity of prawn and premunition.It includes the Chinese herbal medicine active constituent of following mass ratio：Astragalus polyose 40-70%, soluble polysaccharide 10%-20%, sposknikovan 10%-40%, preparation process is simple, and cost is relatively low, easy to use.Prawn immune defense ability can be effectively improved after said preparation production application, prevent and treat disease of prawn, reduce the use of antibiotic and chemicals, the problems such as effective component is indefinite, impurity is more, palatability is poor caused by the addition of Chinese herbal medicine original powder is avoided simultaneously, provides technical guarantee for the green and healthy cultivation of prawn.
Description
Technical field
The present invention relates to a kind of immunopotentiators used for aquiculture, and in particular to a kind of prawn Chinese herbal medicine complex polysaccharide is immune
Enhance feed addictive, belongs to aquatic product matched field of feed additive technology.
Background technique
According to grain agricultural organization of the United Nations（FAO）Statistical data shows, 5,180,000 tons of world's prawn culturing yield in 2016, about
Account for the 6.5% of total aquaculture production in the world.In recent years, shrimp culture industry is primarily present germplasm is degenerated, breeding environment deteriorates etc.
Problem causes prawn immunity function to decline, and disease large-scale outbreak cultivates success rate rapid drawdown, greatly hinders prawn culturing
Industry healthy and sustainable development.Currently, mainly prevented by traditional chemical drug and antibiotic cause of disease outburst, cause environmental pollution,
Causal organism drug resistance, which enhances and passes through food chain, directly endangers human health.
Chinese herbal medicine is the rarity of China's medicinal culture, has green natural, nothing compared to traditional chemical drug and antibiotic
Drug resistance, toxic side effect be small or the advantages such as nothing, resourceful, in aquatic animal disease prevention and treatment with very wide application before
Scape.Currently, traditional Chinese herb feed additive mostly uses original powder to add, that there are effective components is indefinite, impurity is more, palatability difference etc.
Problem, the damage of prawn hepatopancrease and breeding environment pollution can be caused by feeding for a long time in the breeding process also.
Summary of the invention
In view of the above-mentioned problems, the purpose of the present invention is to provide a kind of prawn Chinese herbal medicine complex polysaccharide immunity enhanced feeds to add
Add agent, said preparation selects three kinds of Chinese herbal medicines to extract immune polysaccharide, composition Chinese herbal medicine complex polysaccharide immunity enhanced feed addition respectively
Agent is added in prawn feed, improves immunity of prawn and premunition.The specific technical solution that the present invention uses for：
A kind of prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement, which is characterized in that based on mass fraction, it includes Huang
Astragalus polysaccharides 40-70%, soluble polysaccharide 10%-20%, sposknikovan 10%-40%.
Preferably, the polysaccharides of traditional Chinese medicine immunopotentiator, astragalus polyose：Soluble polysaccharide：The mass ratio of sposknikovan
It is 4：1：1.
Preferably, the polysaccharides of traditional Chinese medicine immunopotentiator, astragalus polyose：Soluble polysaccharide：The mass ratio of sposknikovan
It is 4：1：3.
The astragalus polyose extracts from Radix Astragali, and the soluble polysaccharide extracts from Rhizoma Atractylodis Macrocephalae, and the sposknikovan extracts
From radix saposhnikoviae, extracting method is all made of water extraction, by taking Radix Astragali as an example, after Radix Astragali is uniformly crushed, presses mass fraction 1 with distilled water:
12 after mixing, is decocted 2 times with boiling water, 1.5 hours every time, merges filtrate twice, 95% ethyl alcohol is added after reduced pressure, most
Whole determining alcohol is 70%, is stood overnight, and filters, obtains astragalus polyose crude extract, soluble polysaccharide crude extract and sposknikovan crude extract；
Then conventionally from above-mentioned astragalus polyose crude extract, soluble polysaccharide crude extract and sposknikovan crude extract
It is middle to extract astragalus polyose, soluble polysaccharide and sposknikovan respectively.
A kind of application of the prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement in prawn is immune；It can
Prawn immunity of organisms is significantly improved, prawn premunition is improved.
A kind of prawn feed containing prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement, it is characterised in that the feeding
Material is obtained after adding astragalus polyose, soluble polysaccharide and sposknikovan in basal feed；And add in every kilogram of basal feed
Add astragalus polyose 1.3g, soluble polysaccharide 0.325g and sposknikovan 0.325g or astragalus polyose 1.3g, soluble polysaccharide 0.325g
With sposknikovan 0.975g.
The basal feed based on mass fraction, fish meal 34%, dregs of beans 20%, peanut meal 16.4%, wheat flour 22%, soybean
Phosphatidase 5 %, multi-vitamins 0.6%, composite mineral matter 2%.
The advantages of the present invention：
（1）Improve litopenaeus vannamei haemocyte quantity.Haemocyte is the core component of the immune system of crustacean, is reflected
The immunological stress ability or health status of body.In 28 days for feeding Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement,
Test group prawn haemocyte quantity is all remarkably higher than control group（P<0.05）, and control group is without significant difference.
（2）Improve litopenaeus vannamei haemocyte phagocytic activity.It is outer that haemocyte can eliminate cause of disease etc. by phagocytosis
Carry out substance, therefore, haemocyte phagocytic activity is the important embodiment of prawn immunocompetence, is feeding the immune increasing of Chinese herbal medicine complex polysaccharide
In 28 days of strong feed addictive, test group prawn haemocyte phagocytic activity is all remarkably higher than control group（P<0.05）, and compare
Group is without significant difference.
（3）Improve litopenaeus vannamei blood plasma antibacterial activity.Antibacterial activity reflects the ingredients such as the antibacterial peptide of crustacean
Anti-microbial effect activity, in 28 days for feeding Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement, test group prawn
Blood plasma antibacterial activity is all remarkably higher than control group（P<0.05）, and control group is without significant difference.
（4）Improve litopenaeus vannamei blood plasma Bacteriolytic activity.Bacteriolytic activity has reacted lysozyme activity in crustacean body,
In 28 days for feeding Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement, test group prawn Bacteriolytic activity is all remarkably higher than pair
According to group（P<0.05）, and control group is without significant difference.
（5）Litopenaeus vannamei is improved to the resistivity of morbid vibrio.Challenge test is the result shows that breathe out Vickers in injection
7 days after vibrios, test group prawn cumulative mortality is substantially less than control group（P<0.05）.
Test, which demonstrates Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement provided by the present invention, can enhance prawn
Immunity of organisms and disease resistance；The reinforcing agent selects three kinds of Radix Astragali, Rhizoma Atractylodis Macrocephalae, radix saposhnikoviae Chinese herbal medicines to carry out scientific compatibility, and mention
Its immune polysaccharide is taken, effectively prevents caused by addition Chinese herbal medicine original powder that palatability is poor, hepatopancrease burden increases, destroys and cultivates
The ill effects such as environment；The use that effectively can substitute and reduce traditional chemical drug and antibiotic, it is green to meet aquaculture
Color sound development theory；Effective component preparation of the present invention is simple, and cost is relatively low, can be improved the economic benefit of shrimp culture industry.
Detailed description of the invention
Fig. 1 is the result figure of prawn haemocyte quantity in embodiment 2.
Fig. 2 is the result figure of prawn haemocyte phagocytic activity in embodiment 2.
Fig. 3 is the result figure of prawn blood plasma antibacterial activity in embodiment 2.
Fig. 4 is the result figure of prawn blood plasma Bacteriolytic activity in embodiment 2.
Fig. 5 is the result figure of challenge viral dosage prawn cumulative mortality in embodiment 2.
Specific embodiment
It is specific embodiment of the present invention below, technical solution of the present invention is described further, but this hair
Bright protection scope is not limited to this embodiment.It is all to be included in this hair without departing substantially from the change of present inventive concept or equivalent substitute
Within bright protection scope.
Embodiment 1：
The preparation of prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement and breeding feed
Chinese herbal medicine immune polysaccharide is prepared first, and water extraction is taken in extraction.Respectively by the Radix Astragali of sheet, Rhizoma Atractylodis Macrocephalae, windproof homogeneous powder
After broken, according to 1:12 mass volume ratio is uniformly mixed with distilled water, is decocted extraction 2 times, 1.5 hours every time, is merged 2 filters
Liquid, 95% ethyl alcohol is added after reduced pressure makes final ethanol content 70%, stands overnight, filter up to astragalus polyose crude extract,
Soluble polysaccharide crude extract and sposknikovan crude extract；
Then conventionally from above-mentioned astragalus polyose crude extract, soluble polysaccharide crude extract and sposknikovan crude extract
It is middle to extract astragalus polyose, soluble polysaccharide and sposknikovan respectively.
Add according to the Chinese herbal medicine complex polysaccharide immunity enhanced feed that above-mentioned preparation method prepares two kinds of various concentrations respectively
Add agent and corresponding breeding feed.Based on mass fraction, a kind of Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement includes every
Kilogram of feed adds astragalus polyose 1.3g, soluble polysaccharide 0.325g and sposknikovan 0.325；Another Chinese herbal medicine complex polysaccharide is exempted from
Epidemic disease enhancing feed addictive includes every kilogram of feed addition astragalus polyose 1.3g, soluble polysaccharide 0.325g and sposknikovan
0.975g；
The content of said herbal medicine effective component can be measured according to method provided by existing literature.Astragalus polyose content
Measuring method reference：The comparison of Methods for Determination of Astraglus Polysaccharides in Radix Astragali, Chinese Journal of Pharmaceuticals, 2005 volume 36
9th phase；The measuring method reference of soluble polysaccharide content：Different sources soluble polysaccharide assay, Chinese medicine company, 2005 the 14th
Rolled up for the 4th phase；The measuring method reference of sposknikovan content：The extraction separation of sposknikovan and content assaying method are studied, Jilin
Traditional Chinese medicine, the 5th phase of volume 36 in 2016.
Embodiment 2：
Influence of the prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement to litopenaeus vannamei non-specific immunity.
In this test, the prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement that inventor prepares embodiment 1 is raised
Litopenaeus vannamei is fed, by analyzing the salivary lysozyme of litopenaeus vannamei, the research present invention is to raising litopenaeus vannamei
The effect of immunity.
1 materials and methods
1.1 material
Litopenaeus vannamei is used in test（Litopenaeus vannamei）It is purchased from Laoshan District sand mouth prawn culturing factory of Qingdao City, body
Weight is 3 ± 0.32g grams, and the ingredient of test basal feed is：Based on mass fraction, fish meal 34%, dregs of beans 20%, peanut meal
16.4%, wheat flour 22%, soybean lecithin 5%, multivitamin 0.6%, composite mineral matter 2%.Through nutrition in experimental calculation feed at
It is divided into：Based on mass fraction, crude protein 42.4%, crude fat 7.2%, ash content 8.4%.
1.2 method
1.2.1 experimental design
Test is randomly divided into 3 groups, respectively control group, test group 1 and test group 2 after temporarily being supported 7 days with litopenaeus vannamei, and every group sets
3 repetitions, each repeating groups put 70 tail shrimps in a suitable place to breed.Control group feeds basal feed, and test group 1 and test group 2 feed implementation respectively
Contain prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement feed prepared by example 1.After culture experiment continues 28 days, often
Group takes 30 tail shrimps to carry out challenge test at random, and with 10 tail shrimps for a repetition, each test group 3 is repeated.Vibrio harveyi is pressed 1
×106Cell/mL concentration is in the end intramuscular injection of prawn periproct（30 μ L/ tails）.During test, 2 times are fed daily, day feeding volume
It is the 5% of weight, during test, water temperature, salinity, PH, respectively 22-23 DEG C, 34 ‰, 7.8-8.0.
1.2.2 testing index and method
The 0th after feeding Chinese medicine immunity enhancer, sample within 7,14,21,28 days.Every time litopenaeus vannamei is taken at random for every group
8 tails are inserted directly into 3mm or so in prawn carapace rear cardiocoelom using No. 5 syringe needles and 1mL syringe of disinfection and are adopted
Blood has sucked the improved pre-cooling litopenaeus vannamei anti-coagulants of 0.3mL in syringe before drawing blood in advance（0.34M NaCl, 0.01M
KCl, 0.01M EDTA-Na2, 0.01 M HEPES, pH7.45, osmotic pressure is 780 mOsm.kg-1）, finally make anti-coagulants
Ratio with hemolymph is 1:1.A part of hemolymph sample carries out the measurement of haemocyte quantity and phagocytic rate, remaining hemolymph sample
Product after 800g centrifugation 10min, take blue supernatant, as plasma sample, carry out the survey of antibacterial activity and Bacteriolytic activity at 4 DEG C
It is fixed.
The measurement of haemocyte quantity is counted under an optical microscope using blood counting chamber；The measurement side of phagocytic activity
Method is operated according to the method for existing literature, is referred to：Immune responses and expression of immune-
related genes in swimming crab Portunus trituberculatus exposed to elevated
ambient ammonia-N stress. Comparative Biochemistry and Physiology Part A；Antibacterial
Vigor, Bacteriolytic activity measuring method according to existing literature method operate, be referred to：Insect immunity.
Purification and properties of three inducible bactericidal proteins from
hemolymph of immunized pupae of Hyalophora cecropia. European Journal of
Biochemistry；The measurement of cumulative mortality：Cumulative mortality（%）=prawn death toll/prawn sum × 100.
2 results
2.1 haemocyte quantity
As seen from Figure 1：After feeding 7 days of prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement, blood is thin in prawn body
Born of the same parents' number dramatically increases（P<0.05）, test group 2 reached peak at 14 days, and test group 1 reached peak at 21 days.Cultivation examination
After testing, the haemocyte quantity of test group 1 and test group 2 increases separately 19.5% and 23.6%, is all remarkably higher than control group（P<
0.05）, test group 2 increase sposknikovan feeding volume can further hemocytes increasing quantity, but between test group 1 and test group 2
Difference is not significant（p>0.05）.
2.2 haemocyte phagocytic activity
As seen from Figure 2：After feeding prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement 7 days, the phagocytosis of prawn haemocyte
Activity is all remarkably higher than control group（P<0.05）, during feeding 14 days to 21 days, 2 phagocytic activity of test group is significantly higher than test
Group 1（P<0.05）, but as Feeding time increases, after 28 days, test group 2 and 1 haemocyte phagocytic rate difference of test group are not shown
It writes（p>0.05）.
2.3 antibacterial activity
As seen from Figure 3：After feeding 7 days of prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement, test group 1 and test
The prawn antibacterial activity of group 2 is all remarkably higher than control group（P<0.05）.As Feeding time increases, test group antibacterial activity is always
It is maintained at higher level, the antibacterial activity of test group 2 is higher than test group 1, but difference is not significant（p>0.05）.
2.4 Bacteriolytic activity
As seen from Figure 4：After feeding 7 days of prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement, test group prawn is molten
Bacterium activity is all remarkably higher than control group（P<0.05）.After feeding 14 days, test group 1 and 2 Bacteriolytic activity of test group are respectively reached
Maximum value, after 28 days culture experiments, the Bacteriolytic activity of test group 2 is higher than test group 1, but difference is not significant（p>
0.05）.
2.5 cumulative mortality
As a result see Fig. 5：Control group prawn cumulative mortality is apparently higher than each test group（P<0.05）, at first 3 days, each group death toll
Higher and tend towards stability after 4 days, after challenge viral dosage, control group cumulative mortality is up to 83.2%, and test group 1 is accumulative
The death rate is 43.2%, 2 cumulative mortality 39.8% of test group, shows to feed addition astragalus polyose, soluble polysaccharide and sposknikovan
Feed Energy improve the anti-vibrios ability of litopenaeus vannamei.
Claims (7)
1. a kind of prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement, which is characterized in that based on mass fraction, it includes
Astragalus polyose 40-70%, soluble polysaccharide 10%-20%, sposknikovan 10%-40%.
2. prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement as described in claim 1, which is characterized in that Radix Astragali is more
Sugar：Soluble polysaccharide：The mass ratio of sposknikovan is 4：1：1.
3. prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement as described in claim 1, which is characterized in that Radix Astragali is more
Sugar：Soluble polysaccharide：The mass ratio of sposknikovan is 4：1：3.
4. prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement as claimed in any one of claims 1-3, feature
It is that the astragalus polyose, soluble polysaccharide, sposknikovan are prepared by the following method：Respectively uniformly by Radix Astragali, Rhizoma Atractylodis Macrocephalae, radix saposhnikoviae
After crushing, then mass fraction 1 is pressed with distilled water respectively:12 are uniformly mixed, and are decocted 2 times, 1.5 hours every time, are closed respectively with boiling water
And filtrate twice, 95% ethyl alcohol is added after reduced pressure, final determining alcohol is 70%, is stood overnight, and is filtered respectively more up to Radix Astragali
Sugared crude extract, soluble polysaccharide crude extract and sposknikovan crude extract；
Then conventionally from above-mentioned astragalus polyose crude extract, soluble polysaccharide crude extract and sposknikovan crude extract
It is middle to extract astragalus polyose, soluble polysaccharide and sposknikovan respectively.
5. the described in any item prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement of claim 1-3 are in prawn is immune
Application.
6. a kind of prawn feed containing prawn Chinese herbal medicine complex polysaccharide feedstuff additive for immunity enhancement, it is characterised in that the feed
It is to be obtained after adding astragalus polyose, soluble polysaccharide and sposknikovan in basal feed；And it is added in every kilogram of basal feed
Astragalus polyose 1.3g, soluble polysaccharide 0.325g and sposknikovan 0.325g or astragalus polyose 1.3g, soluble polysaccharide 0.325g and
Sposknikovan 0.975g.
7. prawn feed as claimed in claim 6, it is characterised in that the basal feed based on mass fraction, fish meal 34%,
Dregs of beans 20%, peanut meal 16.4%, wheat flour 22%, soybean lecithin 5%, multi-vitamins 0.6%, composite mineral matter 2%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753852A (en) * | 2021-01-20 | 2021-05-07 | 南京众成正源生物技术有限公司 | Feed additive for improving disease resistance of fishes and shrimps as well as preparation method and application thereof |
| CN113046275A (en) * | 2021-04-27 | 2021-06-29 | 中国海洋大学 | Bacillus used for prawn feed and fermented Chinese herbal medicine preparation used for prawn feed |
| CN114788554A (en) * | 2022-04-06 | 2022-07-26 | 诺立(上海)生物工程有限公司 | Nutritional composition for regulating immunity of aquatic animals and preparation method thereof |
| CN115029267A (en) * | 2022-06-20 | 2022-09-09 | 聚芯生物工程有限公司 | Disease-resistant growth-promoting composite feed additive for prawns and application thereof |
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| CN106215001A (en) * | 2016-08-22 | 2016-12-14 | 常德永利盛祥生物科技有限公司 | For the Chinese medicine composition of freshwater aquiculture commonly encountered diseases and using method thereof and purposes |
| CN106942521A (en) * | 2017-02-28 | 2017-07-14 | 中国海洋大学 | What a kind of property of medicine was stablized is used for prawn Immune-enhancing effect and sterilized Chinese herbal and crude drugs preparations and method |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102146142A (en) * | 2011-03-30 | 2011-08-10 | 泸州品创科技有限公司 | Method for preparing astragalus polysaccharides |
| CN105661105A (en) * | 2016-01-29 | 2016-06-15 | 中国海洋大学 | Compound Chinese herbal medicine immunopotentiator for prawns |
| CN106215001A (en) * | 2016-08-22 | 2016-12-14 | 常德永利盛祥生物科技有限公司 | For the Chinese medicine composition of freshwater aquiculture commonly encountered diseases and using method thereof and purposes |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753852A (en) * | 2021-01-20 | 2021-05-07 | 南京众成正源生物技术有限公司 | Feed additive for improving disease resistance of fishes and shrimps as well as preparation method and application thereof |
| CN113046275A (en) * | 2021-04-27 | 2021-06-29 | 中国海洋大学 | Bacillus used for prawn feed and fermented Chinese herbal medicine preparation used for prawn feed |
| CN114788554A (en) * | 2022-04-06 | 2022-07-26 | 诺立(上海)生物工程有限公司 | Nutritional composition for regulating immunity of aquatic animals and preparation method thereof |
| CN115029267A (en) * | 2022-06-20 | 2022-09-09 | 聚芯生物工程有限公司 | Disease-resistant growth-promoting composite feed additive for prawns and application thereof |
| CN115029267B (en) * | 2022-06-20 | 2022-12-27 | 聚芯生物工程有限公司 | Disease-resistant growth-promoting composite feed additive for prawns and application thereof |
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