CN108801958A - A kind of biochemical substances detecting instrument and its detection method based on optical means - Google Patents
A kind of biochemical substances detecting instrument and its detection method based on optical means Download PDFInfo
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- CN108801958A CN108801958A CN201810863273.7A CN201810863273A CN108801958A CN 108801958 A CN108801958 A CN 108801958A CN 201810863273 A CN201810863273 A CN 201810863273A CN 108801958 A CN108801958 A CN 108801958A
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- 230000003287 optical effect Effects 0.000 title claims abstract description 61
- 239000000126 substance Substances 0.000 title claims abstract description 38
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 238000001228 spectrum Methods 0.000 claims abstract description 28
- 230000003321 amplification Effects 0.000 claims abstract description 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 13
- 238000012360 testing method Methods 0.000 claims abstract description 12
- 230000035945 sensitivity Effects 0.000 claims abstract description 10
- 238000002835 absorbance Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 5
- 238000007405 data analysis Methods 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims 1
- 239000013307 optical fiber Substances 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 41
- 238000004737 colorimetric analysis Methods 0.000 description 5
- 238000013461 design Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 240000005373 Panax quinquefolius Species 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kind of biochemical substances detecting instrument and its detection method based on optical means, belong to biochemical substances detection technique field, including MCU chip, motor driver and LED driver, MCU chip is connect by motor driver with stepper motor, stepper motor is connect with turntable optical filter, MCU chip is connect by LED driver with white light LEDs, turntable optical filter is connect by optical fiber with sample box, photodiode is connect by switching with variable-gain amplification circuit, and variable-gain amplification circuit is connect by ADC collectors with MCU chip.The present invention program-controlled can be switched using the LED light source of full spectrum, the size of its constant current driving, receiving terminal gain；Light-intensity test device has selected high performance OSD-1226 photosensitive sensors, to ensure the sensitivity to special wavelength light；The detected sample sample of various various concentrations is adapted to by that can be selected there are four the optical filter of wavelength.
Description
Technical field
The present invention relates to a kind of biochemical substances detecting instrument and its detection methods, and optical means is based on more particularly to one kind
Biochemical substances detecting instrument and its detection method, belong to biochemical substances detection technique field.
Background technology
There is many chemical substances color, some colourless compounds can also be acted on color developing agent and generate colored substance
Matter, facts proved that, when the concentration of colored solutions changes, the depth of color is also with change, and concentration is bigger, and color is deeper；It is dense
Spend smaller, color is more shallow, therefore, the concentration of colored solutions can be determined by comparing the method for the solution colour depth, to molten
Substance contained in liquid carries out quantitative analysis, such as Na Shi pipe colorimetric methods, it be by concentration from high to low, it is dense to prepare series of standards
Degree pipe, then takes sample to be tested and standard pipe to compare one by one, sees most close with the color of which standard pipe, just reads the standard
The concentration value of pipe is the concentration value of sample to be tested, and here it is optical colorimetries.
It uses optical detection device instead later to replace visually to measure the content of substance in detected solution, this method is photoelectricity
Colorimetric method, so the present invention is also based on improving and optimizating for photoelectric colorimetry and carries out patent application proposition, current instrument, due to
Limited performance indeterminacy or can not provide biochemical indicator in sample in the case where sample concentration is very high or very low, reason
It is main that there are two aspects：
One is using the limitation of light source, light-intensity test device, light source has scarce in certain wavelength especially short wavelength's frequency range
It loses, detection device is low in certain segment sensitive degree.This can cause detect under certain wavelength or detection result is very poor.
The second is light source uses the constant intensity way of output, it can only be directed under intermediate concentration state and optimize, measure dynamic model
Enclose small, sample concentration is low, receives detection device and is easily saturated, sample concentration is high, is limited to sensitivity, it is difficult to differentiate.
Therefore, it in order to solve the problems, such as two above, needs to propose a kind of biochemical substances detecting instrument based on optical means.
Invention content
The main object of the present invention is to provide for a kind of biochemical substances detecting instrument based on optical means, is to be based on object
The polychromatic light that light source is sent out is divided into monochromatic light by the selective absorbing of confrontation light, monochromator, and the monochromatic light of specific wavelength passes through Sheng
Feeding signal processing system is analyzed after having the colorimetric pool of sample solution, photoelectric converter that transmitted light is converted to electric signal.
The purpose of the present invention can reach by using following technical solution：
A kind of biochemical substances detecting instrument based on optical means, including MCU chip and connect respectively with the MCU chip
LED driver for carrying out motor-driven motor driver and being driven for LED, the MCU chip pass through the motor
Driver is connect with stepper motor, and the stepper motor is connect with turntable optical filter, and the MCU chip is driven by the LED
Device is connect with white light LEDs, and the white light LEDs are also connect with the turntable optical filter, and the turntable optical filter passes through optical fiber and sample
Product box connects, and the sample box is connected with switch by photodiode, and the photodiode is by the switch and can be changed
Gain amplifying circuit connects, and the variable-gain amplification circuit is connect by ADC collectors with the MCU chip.
Preferably, the white light LEDs are full spectrum white-light LED, the whole needed for the full spectrum white-light LED coverage test
Wavelength, and have enough luminous intensities under each wavelength.
Preferably, the photodiode is detection device, is sensitivity under each wavelength needed for the full spectrum white-light LED
High OSD1226 type photodiodes, for ensureing that the light of each wavelength needed for test can effectively emit and receive.
Preferably, the electric current of the full spectrum white-light LED is driven to adjust the output brightness of light source by changing.
Preferably, the LED driver drives full spectrum white-light LED using constant current, and the LED driver is most
Large-drive-current is 1.3A.
Preferably, the stepper motor is driven by the motor driver, and the stepper motor rotation is made to carry out rotating dress
There are four types of the turntable optical filters of wavelength filter.
Preferably, the photodiode will transmit through the optical signal of sample box and be converted to electric signal, which passes through institute
It is collected by the ADC collectors after stating variable-gain amplification circuit amplification, the result of acquisition is transferred to by the ADC collectors
The MCU chip carries out data analysis.
A kind of detection method of the biochemical substances detecting instrument based on optical means, includes the following steps：
Detecting instrument is obtained after self-correcting in the case where no sample is put into, and full-spectrum LED is after optical filter
Quiescent value, and record；
After tested sample is put into, detecting instrument obtains having sample to be put into, and full-spectrum LED passes through optical filter
Afterwards, there is load value using after tested sample；
The absorbance that sample is obtained finally by the formula for calculating absorbance, the biochemistry of sample is judged with this
Index.
The advantageous effects of the present invention：Biochemical substances detecting instrument and its inspection provided by the invention based on optical means
Survey method, the size driven using the LED light source of full spectrum, its constant current, receiving terminal gain program-controlled can be switched；Light intensity detector
Part has selected high performance OSD-1226 photosensitive sensors, to ensure the sensitivity to special wavelength light；By the way that there are four wavelength
Optical filter it can be selected that and transmitting, the adjustment of receiving circuit dynamic range, to adapt to the tested all of various various concentrations
Product.
Description of the drawings
Fig. 1 is that the structure of a preferred embodiment of the biochemical substances detecting instrument according to the invention based on optical means is former
Reason figure.
Specific implementation mode
To make the more clear and clear technical scheme of the present invention of those skilled in the art, with reference to embodiment and attached drawing
The present invention is described in further detail, and embodiments of the present invention are not limited thereto.
As shown in Figure 1, the biochemical substances detecting instrument provided in this embodiment based on optical means, including MCU chip and
The LED driver for carrying out motor-driven motor driver and being driven for LED, MCU chip are connect with MCU chip respectively
Connect with stepper motor by motor driver, stepper motor is connect with turntable optical filter, MCU chip by LED driver with
White light LEDs connect, and white light LEDs are also connect with turntable optical filter, and turntable optical filter is connect by optical fiber with sample box, and sample box is logical
It crosses photodiode and is connected with switch, photodiode is connect by switching with variable-gain amplification circuit, variable gain amplification
Circuit is connect by ADC collectors with MCU chip.
In the present embodiment, as shown in Figure 1, white light LEDs are full spectrum white-light LED, full spectrum white-light LED coverage test institute
The whole wavelength needed, and have enough luminous intensities under each wavelength, photodiode is detection device, is full spectrum white-light
The high OSD1226 type photodiodes of sensitivity under each wavelength needed for LED, the light for ensureing each wavelength needed for test
Effectively transmitting and reception drives the electric current of full spectrum white-light LED to adjust the output brightness of light source by changing.
In the present embodiment, as shown in Figure 1, LED driver drives full spectrum white-light LED, LED to drive using constant current
The maximum drive current of dynamic device is 1.3A, and stepper motor is driven by motor driver, and stepper motor rotation is made to be equipped with to rotate
The turntable optical filter of four kinds of wavelength filters, the optical signal that photodiode will transmit through sample box are converted to electric signal, the telecommunications
It number is collected by ADC collectors after variable-gain amplification circuit amplifies, the result of acquisition is transferred to MCU by ADC collectors
Chip carries out data analysis.
In the present embodiment, as shown in Figure 1, the biochemical substances detecting instrument provided in this embodiment based on optical means
Detection method includes the following steps：
Detecting instrument is obtained after self-correcting in the case where no sample is put into, and full-spectrum LED is after optical filter
Quiescent value, and record；
After tested sample is put into, detecting instrument obtains having sample to be put into, and full-spectrum LED passes through optical filter
Afterwards, there is load value using after tested sample；
The absorbance that sample is obtained finally by the formula for calculating absorbance, the biochemistry of sample is judged with this
Index.
In the present embodiment, the biochemical substances detecting instrument provided in this embodiment based on optical means, for can cause
It can not be detected under certain wavelength or disadvantage that detection result is very poor, light source selects the LED of full spectrum, complete needed for coverage test
Portion's wavelength, and have enough luminous intensities under each wavelength, device photodiode is detected, it is same to select under required each wavelength
The high model OSD1226 of sensitivity, it ensure that the light of each wavelength needed for test effectively can emit and receive.
In the present embodiment, as shown in Figure 1, the biochemical substances detecting instrument provided in this embodiment based on optical means,
Small for measurement dynamic range, sample concentration is low, receives detection device is easily saturated, sample concentration height, is limited to sensitivity, is difficult to
The output brightness of the shortcomings that resolution, light source are changed to adjustable form, drive the electric current of LED light source to realize by change, receive
By variable-gain amplification circuit, the signal to adapt to Larger Dynamic range receives circuit, passes through transmitting, receiving circuit dynamic range
Adjustment, to adapt to the tested of various various concentrations, low concentration (high transmittance) sample, light source is adjusted to low light intensity so that inspection
It is unsaturated to survey photodiode；High concentration (low transmission) sample, light source are adjusted to high light intensity, and detection photodiode is made to obtain
Signal output as big as possible, increases detection sensitivity.
In the present embodiment, the biochemical substances detecting instrument provided in this embodiment based on optical means, using constant electricity
Controllable circuit is flowed to drive full spectrum white-light LED, this driving circuit peripheral components is few, and at low cost, driving current is big, maximum
1.3A can be reached, can much be met the requirements, when sample absorbance is high, the range for selecting driving current big works as sample
Absorbance is low, the range for selecting driving current small, and by Driving Stepping Motor rotation, come rotating dress, there are four types of wavelength filters
Turntable selects the optical filter of specific wavelength with this, this for sample excessive concentration or it is too low in the case of, ratio can be measured
Accurate data.
In the present embodiment, the biochemical substances detecting instrument provided in this embodiment based on optical means, photodiode
The optical signal that sample can be will transmit through is converted to electric signal, which can be collected after amplifying circuit by ADC, finally
The result of acquisition can be transferred to MCU and carry out data analysis by ADC.
In the present embodiment, the biochemical substances detecting instrument provided in this embodiment based on optical means, operation principle
It is as follows：Detecting instrument obtains being put into no sample after self-correcting, zero load of the full-spectrum LED after optical filter
Value, and record, after tested sample is put into, detecting instrument obtains having sample to be put into, and full-spectrum LED is through filtering
After mating plate, there is load value using after tested sample, the absorbance of sample obtained finally by the formula for calculating absorbance,
The biochemical indicator of sample is judged with this.
In the present embodiment, the biochemical substances detecting instrument provided in this embodiment based on optical means, using full spectrum
LED light source, its constant current driving size, receiving terminal gain program-controlled can switch.
In the present embodiment, the biochemical substances detecting instrument provided in this embodiment based on optical means, light intensity detector
Part has selected high performance OSD-1226 photosensitive sensors, to ensure the sensitivity to special wavelength light.
In the present embodiment, the biochemical substances detecting instrument provided in this embodiment based on optical means, there are four
The optical filter of wavelength it can be selected that and transmitting, the adjustment of receiving circuit dynamic range, to adapt to the tested of various various concentrations
All product.
In the present embodiment, the biochemical substances detecting instrument provided in this embodiment based on optical means is to be based on substance
To the selective absorbing of light, i.e. spectrophotometric principles, the polychromatic light that light source is sent out is divided into monochromatic light, specific wavelength by monochromator
Monochromatic light be sent into signal processing after transmitted light is converted to electric signal by filling the colorimetric pool of sample solution, photoelectric converter
System is analyzed, i.e., analyzes the biochemical indicator in sample using the principle of photoelectric colorimetry.
In conclusion in the present embodiment, biochemical substances detecting instrument provided in this embodiment based on optical means and
Its detection method, because transmitting terminal and receiving terminal can carry out program-controlled adjustment, the range of this test sample concentration can be expanded, mistake
High or too low sample concentration can be measured accurately, and the photosensitive sensor performance of selection is good, in Instrument Design
Four kinds of specific wavelengths light it is more sensitive, this is particularly important for the accuracy of test result.
The above, further embodiment only of the present invention, but scope of protection of the present invention is not limited thereto, and it is any
Within the scope of the present disclosure, according to the technique and scheme of the present invention and its design adds those familiar with the art
With equivalent substitution or change, protection scope of the present invention is belonged to.
Claims (8)
1. a kind of biochemical substances detecting instrument based on optical means, including MCU chip and use is connect with the MCU chip respectively
In the LED driver for carrying out motor-driven motor driver and being driven for LED, which is characterized in that the MCU chip passes through
The motor driver is connect with stepper motor, and the stepper motor is connect with turntable optical filter, and the MCU chip passes through described
LED driver is connect with white light LEDs, and the white light LEDs are also connect with the turntable optical filter, and the turntable optical filter passes through light
Fibre is connect with sample box, and the sample box is connected with switch by photodiode, and the photodiode passes through the switch
It is connect with variable-gain amplification circuit, the variable-gain amplification circuit is connect by ADC collectors with the MCU chip.
2. a kind of biochemical substances detecting instrument based on optical means as described in claim 1, which is characterized in that the white light
LED is full spectrum white-light LED, whole wavelength needed for the full spectrum white-light LED coverage test, and has foot under each wavelength
Enough luminous intensities.
3. a kind of biochemical substances detecting instrument based on optical means as claimed in claim 2, which is characterized in that described photosensitive
Diode is detection device, is photosensitive two pole of OSD1226 types that sensitivity is high under each wavelength needed for the full spectrum white-light LED
Pipe, for ensureing that the light of each wavelength needed for test can effectively emit and receive.
4. a kind of biochemical substances detecting instrument based on optical means as claimed in claim 2, which is characterized in that pass through change
The electric current of the full spectrum white-light LED is driven to adjust the output brightness of light source.
5. a kind of biochemical substances detecting instrument based on optical means as claimed in claim 2, which is characterized in that the LED
Driver drives full spectrum white-light LED using constant current, and the maximum drive current of the LED driver is 1.3A.
6. a kind of biochemical substances detecting instrument based on optical means as described in claim 1, which is characterized in that the stepping
Motor is driven by the motor driver, and the stepper motor is made to rotate carry out rotating dress there are four types of wavelength filter described turn
Disk optical filter.
7. a kind of biochemical substances detecting instrument based on optical means as described in claim 1, which is characterized in that described photosensitive
The optical signal that diode will transmit through sample box is converted to electric signal, and the electric signal is after variable-gain amplification circuit amplification
It is collected by the ADC collectors, the result of acquisition is transferred to the MCU chip and carries out data analysis by the ADC collectors.
8. a kind of detection side of biochemical substances detecting instrument based on optical means as described in claim 1-7 any one
Method, which is characterized in that include the following steps：
Detecting instrument is obtained after self-correcting in the case where no sample is put into, zero load of the full-spectrum LED after optical filter
Value, and record；
After tested sample is put into, detecting instrument obtains having sample to be put into, full-spectrum LED after optical filter, then
There is load value after tested sample；
It finally by the formula for calculating absorbance obtains the absorbance of sample, judges that the biochemistry of sample refers to this
Mark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810863273.7A CN108801958A (en) | 2018-08-01 | 2018-08-01 | A kind of biochemical substances detecting instrument and its detection method based on optical means |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810863273.7A CN108801958A (en) | 2018-08-01 | 2018-08-01 | A kind of biochemical substances detecting instrument and its detection method based on optical means |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2867341Y (en) * | 2005-12-06 | 2007-02-07 | 沈阳东软医疗系统有限公司 | Spectrophotometer of full-automatic biochemical analyzer |
| CN202974857U (en) * | 2012-12-06 | 2013-06-05 | 力合科技（湖南）股份有限公司 | Spectrophotometric detection device |
| CN107478617A (en) * | 2017-09-04 | 2017-12-15 | 中国计量大学 | Long-range underground water multi-parameter online test method and measurement apparatus |
| CN107782687A (en) * | 2017-12-04 | 2018-03-09 | 江苏博权医疗科技有限公司 | A kind of medical integrated photometer device |
| CN208654021U (en) * | 2018-08-01 | 2019-03-26 | 常州市武进区半导体照明应用技术研究院 | A kind of biochemical substances detecting instrument based on optical means |
-
2018
- 2018-08-01 CN CN201810863273.7A patent/CN108801958A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2867341Y (en) * | 2005-12-06 | 2007-02-07 | 沈阳东软医疗系统有限公司 | Spectrophotometer of full-automatic biochemical analyzer |
| CN202974857U (en) * | 2012-12-06 | 2013-06-05 | 力合科技（湖南）股份有限公司 | Spectrophotometric detection device |
| CN107478617A (en) * | 2017-09-04 | 2017-12-15 | 中国计量大学 | Long-range underground water multi-parameter online test method and measurement apparatus |
| CN107782687A (en) * | 2017-12-04 | 2018-03-09 | 江苏博权医疗科技有限公司 | A kind of medical integrated photometer device |
| CN208654021U (en) * | 2018-08-01 | 2019-03-26 | 常州市武进区半导体照明应用技术研究院 | A kind of biochemical substances detecting instrument based on optical means |
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Application publication date: 20181113 |
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