CN209589846U - Fluorescence detector Stability Determination device - Google Patents
Fluorescence detector Stability Determination device Download PDFInfo
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- CN209589846U CN209589846U CN201920016188.7U CN201920016188U CN209589846U CN 209589846 U CN209589846 U CN 209589846U CN 201920016188 U CN201920016188 U CN 201920016188U CN 209589846 U CN209589846 U CN 209589846U
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Abstract
The utility model relates to a kind of fluorescence detector Stability Determination devices, the device has a shell, one standard optical sensor, one standard sources, shell internal upper part is equipped with the installation groove of left and right two for placing fluorescence detector, two installation grooves are respectively arranged below with standard sources, standard optical sensor, it is equipped with attenuator respectively above standard sources and standard optical sensor, standard sources connects host computer by master control borad with standard optical sensor, and host computer connects fluorescence detector by master control borad.The utility model measures the stability of fluorescence detection module using the standard sources simulation week fluorescent of preposition attenuator using the stability of standard optical sensor measurement fluorescence detector excitation light source.
Description
Technical field
The utility model relates to a kind of optical detection and analysis instrument more particularly to a kind of fluorescence detector Stability Determinations
Device.
Background technique
With economic rapid development, the concern in the fields such as people's environmental pollution, hygiene and health, food safety is increasingly
More, these fields, which are generally required, carries out qualitative or quantitative analysis to it according to the substance characteristics of object to be detected.As qualitative or fixed
The important method of amount analysis substance characteristics, fluorescence analysis method have the characteristics that high sensitivity, the good, signal stabilization of selectivity.It is real
The critical component of existing this method is fluorescence detector, has been widely used in fluorescent PCR instrument as nucleus module and 96 orifice plates are glimmering
Light analyzer etc. is biochemical in environmental analysis instrument, realizes quickly detection to chemical pollutant in food and water and pathogenic microorganisms
Equipment and technical guarantee be provided, and can environmental pollution and food safety quick response, prevent pollution and insecurity factor into
One step expands, and provides safeguard for the healthy living of the mankind.
In recent years, the rapid development of domestic analysis instrument industry, the fluorescence detector of one of critical component tended to be integrated
The overall development of change, modularization, industrialization, fluorescence detector is still in development phase, although its Key Performance Indicator is just
Chasing, even more than foreign technology is horizontal, but engineering effect is not satisfactory, since fluorescence detector itself exists for a long time
Drift causes most fluorescence detections to be at present still in the relative measurement stage.The above problem not only gives production fluorescence detection equipment
Producer bring many difficulties, also to using fluorescence detection carry out quantitative analysis user bring inconvenience.
Summary of the invention
In order to realize relatively accurate fluorescence detection quantitative analysis, guarantee the reliability of fluorescence detection result, it is necessary to right
The stability of fluorescence detector itself is measured, and the utility model provides a kind of fluorescence detector Stability Determination device.
To achieve the above object, the technical solution of the utility model is: a kind of fluorescence detector Stability Determination device, tool
There is the shell of an installation measurement device, one for measuring the standard light sensing of fluorescence detector excitation light source stability
Device, one is used to measure the standard sources of fluorescence detector fluorescence detection stability, and the shell internal upper part is equipped with for putting
The installation groove of left and right two of fluorescence detector is set, two installation grooves are respectively arranged below with standard sources, standard optical sensor,
It is equipped with attenuator respectively above standard sources and standard optical sensor, standard sources is connected with standard optical sensor by master control borad
Host computer, host computer connect fluorescence detector by master control borad.
When fluorescence detector is installed on right side installation groove, the host computer controls fluorescence detector by master control borad
Excitation light source module sending exciting light, the photosensitive element of exciting light alignment criteria optical sensor after attenuator is decayed, and by
Standard optical sensor sensing, and then acquired by master control borad and be uploaded to host computer and be analyzed and processed.
When fluorescence detector is installed on left side installation groove, the host computer controls standard sources by master control borad and exports
Light, the week fluorescent that the light is simulated after attenuator is decayed enter the fluorescence detection module of fluorescence detector, and glimmering
Light detection module receives, and then is acquired by master control borad and be uploaded to host computer and be analyzed and processed.
In continuous mode, the attenuator is replaceable attenuator, eliminates standard light sensing by replacement attenuator
The influence that device or standard sources itself are drifted about to measurement result.
The utility model has the beneficial effects that:
(1) the utility model is flexible in application, can be used for the Stability Determination of different channel fluorescence detectors.
By taking visible spectrum as an example, traditional standard optical sensor and standard sources can cover substantially, therefore channel is swashed
Hair and emission spectrum design the fluorescence detector in visible-range, and no replacement is required standard optical sensor and standard sources are
The Stability Determination of different channel fluorescence detectors can be achieved.For the fluorescence detector of other spectral coverages, according to channel excitation and
Emission spectrum design reasonably selects the matched standard optical sensor of spectral region and standard sources, and Stability Determination can be realized.
(2) in the design production phase, core component is screened using the utility model, in core component level
The upper preliminary performance for guaranteeing fluorescence detector is stablized.
The core of fluorescence detector is two modules of excitation light source and fluorescence detection, therefore excitation light source and photoelectric sensor
Performance by the stability of extreme influence fluorescence detector.In the design production phase, expired using the utility model screenability
The core component required enough can tentatively guarantee that the performance of fluorescence detector is stablized in core component level.
Detailed description of the invention
Fig. 1 is fluorescence detector Stability Determination device；
Wherein: 1. fluorescence detectors；2. installation groove；3. standard optical sensor；4. standard sources；5. attenuator；6. main
Control plate；7. shell；8. host computer；11. excitation light source module；12. fluorescence detection module；31. photosensitive element；
Fig. 2 is focusing fluorescence detector schematic diagram altogether.
Specific embodiment
The preferable example that the utility model is provided below with reference to Fig. 1, is mainly described in further detail, rather than is used to
Limit the scope of the utility model.
Fluorescence detector is mainly made of excitation light source module and fluorescence detection module two parts, the stabilization of the two modules
Performance is most important for the Real_time quantitative detection of fluorescence.Therefore, the utility model technology resolving ideas is as follows:
As shown in Figure 1, a kind of fluorescence detector Stability Determination device, including fluorescence detector 1, installation groove 2, standard
Optical sensor 3, standard sources 4, attenuator 5, master control borad 6, shell 7 and host computer 8.
Standard optical sensor 3, standard sources 4, attenuator 5 and master control borad 6 are mounted in shell 7, and wherein attenuator 5 is installed
Before standard sources 4 and standard optical sensor 3；There are two installation grooves 2, fluorescence detector 1 to pacify for design above shell 7
Loaded in installation groove 2；Host computer 8 is placed in outside shell 7.
When fluorescence detector 1 is installed on right side installation groove 2, host computer 8 controls fluorescence detector 1 by master control borad 6
Excitation light source module 11 issues exciting light, this exciting light can be with the sense of alignment criteria optical sensor 3 after the decaying of attenuator 5
Optical element 31, and sensed by standard optical sensor 3, and then acquired by master control borad 6 and be uploaded to host computer 8 and be analyzed and processed.
When fluorescence detector 1 is installed on left side installation groove 2, host computer 8 controls standard sources 4 by master control borad 6 and exports
Light, the week fluorescent that this light is simulated after the decaying of attenuator 5 can enter the fluorescence detection module of fluorescence detector 1
12, and received by fluorescence detection module 12, and then acquired by master control borad 6 and be uploaded to host computer 8 and be analyzed and processed.
The measuring method of fluorescence detector Stability Determination device described in the utility model comprises the following steps that
(1) fluorescence detector 1 is installed in the installation groove 2 of right side, fluorescence is controlled by master control borad 6 using host computer 8
11 output intensity of excitation light source module of detector 1 is I0Exciting light；
(2) it is τ that transmitance is installed before standard optical sensor 31Attenuator 5, the photosensitive element 31 of standard optical sensor 3
It receives excitation light source module 11 and exports the exciting light after decaying, acquired by master control borad 6 and be uploaded to host computer 8；
(3) host computer 8 receives the data DN value of the sensing of storage standard optical sensor 3 for a long time, and it is saturating that installation is calculated
Crossing rate is τ1Attenuator 5 when average DN value,Since the optical filter bandwidth of fluorescence detector excitation light source module 11 is
20nm, therefore DN value and the linear linear coefficient of excitating light strength I that ideally standard optical sensor 3 responds are K,
Consider further that itself drift delta I of the attenuator 5 and excitation light source module 11 placed before standard optical sensor 31With standard light
The drift delta DN of sensor 31, the response of available 3 i-th of standard optical sensor acquisition is
DN1i=K τ1(I0+ΔI1i)+ΔDN1i (1)
And then it is by the calculation formula that formula 1 acquires average DN value
(4) it is τ that transmitance is installed before standard optical sensor 32τ2>τ1Attenuator 5, repeat step 2~3, be calculated
Installation transmitance is τ2Attenuator 5 when average DN value,And it similarly derivesCalculation formula be
(5) light intensity output fixed for fluorescence detector is I0Excitation light source and standard optical sensor 3, identical one is long
It is considered that the drift of the two is fixed, i.e., in the section time With
It can be calculated by the storing data of host computer 8, then elimination standard optical sensor 3 can be calculated by formula 2 and formula 3 and floated
It movesItself drift of excitation light source afterwardsFor
The excitation light source module 11 for characterizing fluorescence detector 1 exports the stability of excitation light source；
(6) fluorescence detector 1 is installed in the installation groove 2 of left side, standard is controlled by master control borad 6 using host computer 8
4 output intensity of light source is I0' light；
(7) it is τ that transmitance is installed before standard sources 43Attenuator 5, standard sources 4 by attenuator 5 decaying after institute
The fluorescence of simulation enters the fluorescence detection module 12 of fluorescence detector 1, is received by fluorescence detection module 12, and then by master control borad 6
It acquires and is uploaded to host computer 8；
(8) host computer 8 receives the data DN value that storage fluorescence detection module 12 senses for a long time, and it is saturating that installation is calculated
Crossing rate is τ3Attenuator 5 when average DN value,Due to the optical filter bandwidth of fluorescence detector fluorescence detection module 12
For 20nm, therefore the DN value that responds of ideal situation fluorescence detection module 12 and the linear linear coefficient of fluorescence intensity I are K',
Consider further that itself drift delta DN of the attenuator 5 and fluorescence detection module 12 placed before standard sources 43With standard sources 4
Drift delta I3, the response of available 12 i-th of fluorescence detection module acquisition is
DN3i=K' τ3(I0'+ΔI3i)+ΔDN3i (5)
And then it is by the calculation formula that formula 5 acquires average DN value
(9) it is τ that transmitance is installed before standard sources 44τ4>τ3Attenuator 5, repeat step 7~8, installation is calculated
Transmitance is τ4Attenuator 5 when average DN value,And it similarly derivesCalculation formula be
It (10) is I for fixed light intensity output0' standard sources 4 and fluorescence detection module 12, identical distance
The interior drift that can consider the two is fixed, i.e.,WithIt can be with
It is calculated by the storing data of host computer 8, then elimination standard sources 4 can be calculated by formula 6 and formula 7 and drifted aboutAfterwards
Fluorescence detection module 12 itself driftFor
Characterize the stability of the fluorescence detection module 12 of fluorescence detector 1.
The utility model designs fluorescence detector Stability Determination device, measures fluorescence detector using standard optical sensor
The stability of excitation light source, using the standard sources simulation week fluorescent of preposition attenuator to measure the steady of fluorescence detection module
Qualitative energy, and in two continuous modes, the technical solution for being all made of replacement attenuator eliminates standard optical sensor or mark
It is quasi-optical to be originated from the influence that body drifts about to measurement result effectively to measure the stability of fluorescence detector itself, for quantifying for fluorescence
It tests and analyzes and reliable basis is provided.It is implemented as follows:
The utility model is applied to the Stability Determination of focusing fluorescence detector altogether as shown in Figure 2, fluorescence inspection
Device design is surveyed in the channel FAM, excitation wavelength 465nm, launch wavelength 530nm.The light of standard optical sensor selection TI company
Sensor, model OPT301,400~1000nm of spectral region.The irradiation of standard sources selection Hangzhou You Lai Science and Technology Ltd.
Spend standard lamp, model HL-2000, spectral region 360nm~2500nm, stability ± 0.2%/h, standard value can trace to the source China meter
It measures institute (NIM).The attenuator of long rich scientific and technological (Beijing) Co., Ltd of attenuator selection, model K9-ZXLGP, spectral region 250~
700nm, transmitance τ1=79%, τ2=90%, τ3=3.2%, τ4=5%.Master control borad, as main control chip, is led to using STM32
It crosses serial ports and fluorescence detector communicates, communicated by network interface and host computer.Upper computer software is compiled using LabVIEW graphical language
Journey.
Fluorescence detector is installed in the installation groove of right side, it is defeated by master control borad control fluorescence detector using host computer
Intensity is I out0Exciting light, installed respectively before standard optical sensor OPT301 transmitance be τ1And τ2Optical filter, mark
The photosensitive element of light sensor OPT301 receives the exciting light of fluorescence detector output, and is uploaded to by master control borad acquisition upper
Machine is analyzed and processed.After the data that host computer senses OPT301 calculate the drift of elimination standard optical sensor according to formula (4)
Itself drift of excitation light sourceThisThat is the stability of characterization fluorescence detector excitation light source module output excitation light source.
Fluorescence detector is installed in the installation groove of left side, standard sources HL- is controlled by master control borad using host computer
2000 output intensities are I0' light, respectively by transmitance be τ3And τ4Optical filter decaying after the week fluorescent simulated enter it is glimmering
The fluorescence detection module of photodetector, is received by fluorescence detection module, and then is acquired by master control borad and be uploaded to host computer progress
Analysis processing.The data of fluorescence detection module senses are calculated according to formula (8) and eliminate fluorescence after standard sources is drifted about by host computer
Itself drift of detection moduleThisCharacterize the stability of fluorescence detector fluorescence detection module.
Claims (4)
1. a kind of fluorescence detector Stability Determination device, the shell (7) with an installation measurement device, one for measuring
The standard optical sensor (3) of fluorescence detector excitation light source stability, one steady for measuring fluorescence detector fluorescence detection
The standard sources (4) of qualitative energy, it is characterised in that: shell (7) internal upper part is equipped with the left side for placing fluorescence detector (1)
Right two installation grooves (2), two installation grooves (2) are respectively arranged below with standard sources (4), standard optical sensor (3), standard
It is equipped with respectively attenuator (5) above light source (4) and standard optical sensor (3), standard sources (4) and standard optical sensor (3) are logical
Master control borad (6) connection host computer (8) is crossed, host computer (8) passes through master control borad (6) connection fluorescence detector (1).
2. fluorescence detector Stability Determination device according to claim 1, it is characterised in that: when fluorescence detector (1)
When being installed on right side installation groove (2), the host computer (8) controls the excitation light source of fluorescence detector (1) by master control borad (6)
Module (11) issues exciting light, the photosensitive element of exciting light alignment criteria optical sensor (3) after attenuator (5) decay
(31), it and by standard optical sensor (3) senses, and then is acquired by master control borad (6) and be uploaded to host computer (8).
3. fluorescence detector Stability Determination device according to claim 1, it is characterised in that: when fluorescence detector (1)
When being installed on left side installation groove (2), the host computer (8) controls standard sources (4) output light by master control borad (6), described
Light week fluorescent for being simulated after attenuator (5) decay enters the fluorescence detection module (12) of fluorescence detector (1), and by
Fluorescence detection module (12) receives, and then is acquired by master control borad (6) and be uploaded to host computer (8).
4. fluorescence detector Stability Determination device according to claim 2 or 3, it is characterised in that: in continuous mode,
The attenuator (5) is replaceable attenuator, eliminates standard optical sensor (3) or standard sources by replacement attenuator
(4) itself influence of the drift to measurement result.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920016188.7U CN209589846U (en) | 2019-01-04 | 2019-01-04 | Fluorescence detector Stability Determination device |
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| CN201920016188.7U CN209589846U (en) | 2019-01-04 | 2019-01-04 | Fluorescence detector Stability Determination device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109668865A (en) * | 2019-01-04 | 2019-04-23 | 上海科源电子科技有限公司 | Fluorescence detector Stability Determination device and measuring method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109668865A (en) * | 2019-01-04 | 2019-04-23 | 上海科源电子科技有限公司 | Fluorescence detector Stability Determination device and measuring method |
| CN109668865B (en) * | 2019-01-04 | 2024-08-16 | 上海科源电子科技有限公司 | Fluorescence detector stability measuring device and measuring method |
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