CN108796095A - A kind of selection improving carp feed efficiency - Google Patents

A kind of selection improving carp feed efficiency Download PDF

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CN108796095A
CN108796095A CN201810788076.3A CN201810788076A CN108796095A CN 108796095 A CN108796095 A CN 108796095A CN 201810788076 A CN201810788076 A CN 201810788076A CN 108796095 A CN108796095 A CN 108796095A
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selection
primer pair
carp
primer sequence
primer
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CN108796095B (en
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鲁翠云
张晓峰
李超
曹顶臣
郑先虎
程磊
孙效文
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Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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Abstract

The present invention provides a kind of selection improving carp feed efficiency for the traditional breeding technology problem big to feed conversion rate character inheritance improvement difficulty.Selection：One, carp selection and breeding basic population is established；Two, basic population carp genomic DNA is extracted；Three, using the genomic DNA of extraction as template, PCR amplification is carried out with primer pair；Four, according to amplification Selection parent group；Five, parental population combo is bred.The present invention establishes the high transformation efficiency strain of feed according to the genotype of parent, and accuracy is high, realizes the improvement to feed conversion rate character.Moreover, the method for the present invention selection and breeding standard is simple, the later stage only needs detection whose body weight, avoids cumbersome measurement work.

Description

A kind of selection improving carp feed efficiency
Technical field
The present invention relates to a kind of selections improving feed efficiency.
Background technology
Feed conversion rate (feed conversion ratio, FCR) character is to weigh animal feed utilization ratio height One Important Economic technical indicator is increased weight with feed than (Feed to Gain Ratio, F/G) or gain feed ratio (Gain to Feed Ratio, G/F) it indicates.In aquaculture industry, feed cost accounts for about the 65%~75% of cultivation totle drilling cost；Therefore, The feed efficiency of aquaculture Mesichthyes is improved, feed cost is reduced, there is important meaning to increasing aquaculture benefit Justice.But fish the characteristics of living in water, so that the difficulty of accurate quantification feed conversion rate character is increased, needs to single Body is fed for a long time, the data such as periodic measurement and record weightening, food consumption, residual bait.
Carp is the important large freshwater aquiculture kind in China.Systematic breeding, selection cross are utilized for characters such as growth, builds The characteristics of waiting traditional breeding technologies to cultivate all multi items, but being difficult to quantify due to feed conversion rate character utilizes tradition to select It is very big to the difficulty of feed conversion rate character progress genetic improvement to educate technology.
Invention content
The present invention provides one for the traditional breeding technology problem big to feed conversion rate character inheritance improvement difficulty Kind improves the selection of carp feed efficiency.
The selection for improving carp feed efficiency carries out according to the following steps：
One, carp selection and breeding basic population is established；
Two, basic population carp genomic DNA is extracted；
Three, using the genomic DNA of extraction as template, PCR amplification is carried out with primer pair；Wherein, the upstream of primer pair S2 is drawn Object sequence is 5 '-GGAGGAGAGCGATCAAGACA-3 ', downstream primer sequence 5 '-TGGGATTCTGGGTGTTTGGT-3 '； The upstream primer sequence of primer pair S6 is 5 '-AGGTCTCACAGTCTGCACAA-3 ', downstream primer sequence 5 '- TCAGGCTCCAAAACACACTAAT-3'；The upstream primer sequence of primer pair S7 is 5 '-TCACCGACTTTACTGCATACA- 3 ', downstream primer sequence 5 '-CAGACTTACGGAGCAGAAGAA-3 '；The upstream primer sequence of primer pair S11 is 5 '- CTCTAATGCGACTGGGACGT-3 ', downstream primer sequence 5 '-GGCCTCACATTCTTCACAGC-3 '；Primer pair S12's Upstream primer sequence is 5 '-GCACTCCCTCAATAAATACACCA-3 ', downstream primer sequence 5 '- TGCTTGACTAATGCCTGACA-3'；The upstream primer sequence of primer pair S13 is 5 '-TGTTCATTTGCTTTGGACTG-3 ', Downstream primer sequence is 5 '-CACGCTTCTGGTCTTCATTATCTG-3 '；
Four, selection primer pair S2 amplifies GGAGGAGAGCGATCAAGACAGTGATGTGACTCAAATAGAGACAGAAGG ACAGTCTGTGTCCATATTTGGACCAGGAACACCAAGATGGTGTGATGGTGGCCACCATGCACATGAAGACAATGACA GCAATGAAACTCAAATAGAGACTGAGGGAACATCTATGTCCGTGTTTGGGCCAGGAACACCAAACAAGCAGTTTACA ACTCAAGACTTGAACAGTACCAAACACCCAGAATCCCA or primer pair S6 amplify AGGTCTCACAGTCTGCACAACAC CTCATACACACACTCACACATTTAAACACAGATGCACTCCTGCACACATACATGCACATAAAAACTCACACATGCAA ATCTATTCCAACACTGCAGACTAAACACAAACACAAGTTGATGCATCTATATCTTTGGCACAGATGATAAATTAATG GTCCCAAGAGATACCAAATCTCTTATTAGTGTGTTTTGGAGCCTGA or primer pair S7 are amplified TCACCGACTTTACTGCATACATTAGCTGAATTTGAACATCCCATTTGTGTTGTGTTGTCAATATATTCAACTTATGG CTCGTCACACAAGTTTTTTCTCATGCAAGATTGTGTATTTGTACTTTTTGTGACTTTGTCTATAAACATTTAGTAAA CAGCAGAATTTTTTTTTAATAAAATTATGTGTGAAATAGTTTTGTTAGCATATTTC TTCTGCTCCGTAAGTCTG or Primer pair S11 amplifies CTCTAATGCGACTGGGACGTACAAAACTATCGCATCTAAAGCACACTCAGAACTTC ACTGTT ATCAGATACAATGCAAGTACTTTTCAATCAGTGATGAAACAAACTATATCCGACTTGGTTGTTTTCATCACTTTTAC TCCACTGGAACCTTCCTGAAGAAACAGTCTGTAGATGATGGGACTTGCTGCAGTGGCAACTGAAAGTTTAGCTGTGA AGAATGTGAGGCC or primer pair S12 amplify GCACTCCCTCAATAAATACACCATTAAATATTTCCCATATATATTTC CAATATATTAAAAGAAAAAACTAAACAAAACACATTTAAATAGGACAACAAAATACAAGTTGGCTCAATAAAAGAAG AAAAAGATACGTGTTATGATTTTAAAACAACTTTTTTTCTCTCTCTCTTACAATTAGACTTCTTAATCTAACATTGT TAAGGATATTGTTGTCAGGCATTAGTCAAGCA or primer pair S13 amplify TGTTCATTTGCTTTGGACTGATCGTCAT GAGCAGTCAGAAGAAAACACAGAAAAATACTGGCCCATATGGTGCTCTTCTGATACTTCTCGGATTAAGTCACGTTT GGCAAGTCCCGGCTTGTGATGTTACAGAGAGCTGTCGGTCGTGCTCTGAGTTTTCTCCTCTGTACTTGAACAGCTCA ACAGAGGGCAATAGAGAGGCGAGCGACAGCAGGGCATGATATGGGCAAGCCTCCTCGACAGCTCCTGGAAGACGTTC TTAAAAGTTCCTCTAAGTGCTGAAGAAGAGAAGTAGAATATGAATGGATCGAGGCACGCATTGAACGTGCTGGTGAG CAGCGCAAACACTCGCCAGTCGGGGCTGTAATTGCCTACATATCCAACCACGTGTGAGACGTTATAGGGCATGAAGC The homozygote parent of AGATAATGAAGACCAGAAGCGTG is as parental population；
Five, parental population combo is bred, and selection and breeding are completed in filial generation sexal maturity.
The present invention establishes the high transformation efficiency strain of feed according to the genotype of parent, and accuracy is high, realizes to food conversion The improvement of rate character.Moreover, the method for the present invention selection and breeding standard is simple, the later stage only needs detection whose body weight, avoids cumbersome survey Measure work.
Specific implementation mode
Technical solution of the present invention is not limited to act specific implementation mode set forth below, further includes between each specific implementation mode Arbitrary combination.
Specific implementation mode one：The selection that present embodiment improves carp feed efficiency carries out according to the following steps：
One, carp selection and breeding basic population is established；
Two, basic population carp genomic DNA is extracted；
Three, using the genomic DNA of extraction as template, PCR amplification is carried out with primer pair；Wherein, the upstream of primer pair S2 is drawn Object sequence is 5 '-GGAGGAGAGCGATCAAGACA-3 ', downstream primer sequence 5 '-TGGGATTCTGGGTGTTTGGT-3 '； The upstream primer sequence of primer pair S6 is 5 '-AGGTCTCACAGTCTGCACAA-3 ', downstream primer sequence 5 '- TCAGGCTCCAAAACACACTAAT-3'；The upstream primer sequence of primer pair S7 is 5 '-TCACCGACTTTACTGCATACA- 3 ', downstream primer sequence 5 '-CAGACTTACGGAGCAGAAGAA-3 '；The upstream primer sequence of primer pair S11 is 5 '- CTCTAATGCGACTGGGACGT-3 ', downstream primer sequence 5 '-GGCCTCACATTCTTCACAGC-3 '；Primer pair S12's Upstream primer sequence is 5 '-GCACTCCCTCAATAAATACACCA-3 ', downstream primer sequence 5 '- TGCTTGACTAATGCCTGACA-3'；The upstream primer sequence of primer pair S13 is 5 '-TGTTCATTTGCTTTGGACTG-3 ', Downstream primer sequence is 5 '-CACGCTTCTGGTCTTCATTATCTG-3 '；
Four, selection primer pair S2 amplifies GGAGGAGAGCGATCAAGACAGTGATGTGACTCAAATAGAGACAGAAGG ACAGTCTGTGTCCATATTTGGACCAGGAACACCAAGATGGTGTGATGGTGGCCACCATGCACATGAAGACAATGACA GCAATGAAACTCAAATAGAGACTGAGGGAACATCTATGTCCGTGTTTGGGCCAGGAACACCAAACAAGCAGTTTACA ACTCAAGACTTGAACAGTACCAAACACCCAGAATCCCA or primer pair S6 amplify AGGTCTCACAGTCTGCACAACAC CTCATACACACACTCACACATTTAAACACAGATGCACTCCTGCACACATACATGCACATAAAAACTCACACATGCAA ATCTATTCCAACACTGCAGACTAAACACAAACACAAGTTGATGCATCTATATCTTTGGCACAGATGATAAATTAATG GTCCCAAGAGATACCAAATCTCTTATTAGTGTGTTTTGGAGCCTGA or primer pair S7 are amplified TCACCGACTTTACTGCATACATTAGCTGAATTTGAACATCCCATTTGTGTTGTGTTGTCAATATATTCAACTTATGG CTCGTCACACAAGTTTTTTCTCATGCAAGATTGTGTATTTGTACTTTTTGTGACTTTGTCTATAAACATTTAGTAAA CAGCAGAATTTTTTTTTAATAAAATTATGTGTGAAATAGTTTTGTTAGCATATTTC TTCTGCTCCGTAAGTCTG or Primer pair S11 amplifies CTCTAATGCGACTGGGACGTACAAAACTATCGCATCTAAAGCACACTCAGAACTTC ACTGTT ATCAGATACAATGCAAGTACTTTTCAATCAGTGATGAAACAAACTATATCCGACTTGGTTGTTTTCATCACTTTTAC TCCACTGGAACCTTCCTGAAGAAACAGTCTGTAGATGATGGGACTTGCTGCAGTGGCAACTGAAAGTTTAGCTGTGA AGAATGTGAGGCC or primer pair S12 amplify GCACTCCCTCAATAAATACACCATTAAATATTTCCCATATATATTTC CAATATATTAAAAGAAAAAACTAAACAAAACACATTTAAATAGGACAACAAAATACAAGTTGGCTCAATAAAAGAAG AAAAAGATACGTGTTATGATTTTAAAACAACTTTTTTTCTCTCTCTCTTACAATTAGACTTCTTAATCTAACATTGT TAAGGATATTGTTGTCAGGCATTAGTCAAGCA or primer pair S13 amplify TGTTCATTTGCTTTGGACTGATCGTCAT GAGCAGTCAGAAGAAAACACAGAAAAATACTGGCCCATATGGTGCTCTTCTGATACTTCTCGGATTAAGTCACGTTT GGCAAGTCCCGGCTTGTGATGTTACAGAGAGCTGTCGGTCGTGCTCTGAGTTTTCTCCTCTGTACTTGAACAGCTCA ACAGAGGGCAATAGAGAGGCGAGCGACAGCAGGGCATGATATGGGCAAGCCTCCTCGACAGCTCCTGGAAGACGTTC TTAAAAGTTCCTCTAAGTGCTGAAGAAGAGAAGTAGAATATGAATGGATCGAGGCACGCATTGAACGTGCTGGTGAG CAGCGCAAACACTCGCCAGTCGGGGCTGTAATTGCCTACATATCCAACCACGTGTGAGACGTTATAGGGCATGAAGC The homozygote parent of AGATAATGAAGACCAGAAGCGTG is as parental population；
Five, parental population combo is bred, F1Selected under the conditions of same feeding weight it is big, sexal maturity complete selection and breeding.
Present embodiment step 4 determines genotype with the nucleotide for being labelled with underline position in primer pair amplifies sequence.
Specific implementation mode two：The difference of present embodiment and specific implementation mode one is：Step 1 is according to Phenetic Shape establishes carp selection and breeding basic population.Other steps and parameter are identical as embodiment one.
Specific implementation mode three：The difference of present embodiment and specific implementation mode one or two is：Step 2 acquires base Plinth group carp isozyme simultaneously extracts genomic DNA.Other steps and parameter are identical as embodiment one or two.
Embodiment 1
Improve the selection and breeding of carp feed efficiency：
One, carp selection and breeding basic population is established：
The mirror carp parent individual of a pair of of genetic distance of selection moderate (0.5~0.7) establishes family full-sibs, and cultivation is to initially 141 tail individuals point are supported in 0.5m when weight is 20.74 ± 5.28g3In aquarium, daily bait throwing in 3 times is subject to and is satiated with food, and receives Collect residual bait, continuously raising three months, monthly periodic measurement, record weight and food consumption；
Two, basic population carp genomic DNA is extracted：
The blood for acquiring individual, with blood extracts kit (Tiangeng biology) extraction genomic DNA；
Three, the genomic DNA of extraction is diluted to 50ng/ μ L as template, PCR amplification is carried out with primer pair；
Wherein, the upstream primer sequence of primer pair S2 is 5 '-GGAGGAGAGCGATCAAGACA-3 ', downstream primer sequence For 5 '-TGGGATTCTGGGTGTTTGGT-3 '；The upstream primer sequence of primer pair S6 is 5 '-AGGTCTCACAGTCTGCACAA- 3 ', downstream primer sequence 5 '-TCAGGCTCCAAAACACACTAAT-3 '；The upstream primer sequence of primer pair S7 is 5 '- TCACCGACTTTACTGCATACA-3 ', downstream primer sequence 5 '-CAGACTTACGGAGCAGAAGAA-3 '；Primer pair S11 Upstream primer sequence be 5 '-CTCTAATGCGACTGGGACGT-3 ', downstream primer sequence 5 '- GGCCTCACATTCTTCACAGC-3'；The upstream primer sequence of primer pair S12 is 5 '-GCACTCCCTCAATAAATACACCA- 3 ', downstream primer sequence 5 '-TGCTTGACTAATGCCTGACA-3 '；The upstream primer sequence of primer pair S13 is 5 '- TGTTCATTTGCTTTGGACTG-3 ', downstream primer sequence 5 '-CACGCTTCTGGTCTTCATTATCTG-3 '；
15 μ L of PCR reaction systems, wherein 1 μ L of DNA profiling, 10 × buffer, 1.5 μ L, MgCl2(25mmol/L)1.5μL、 0.3 μ L of dNTP (10mmol/L), 0.25 μ L of sense primer (10umol/L), 0.25 μ L of downstream primer (10umol/L), Taq enzyme (5U/ μ L) 0.25 μ L, aseptic deionized water are supplemented to 15 μ L；
PCR response procedures are 94 DEG C of denaturation 3min；94 DEG C of denaturation 15s, 56 DEG C of annealing 15s, 72 DEG C of extension 30s, 35 are followed Ring；Last 72 DEG C of extensions 3min；
Polymorphic site is identified using the SNaPshot SNP typing methods based on fluorescent marker Single base extension principle.
Four, selection primer pair S2 amplifies GGAGGAGAGCGATCAAGACAGTGATGTGACTCAAATAGAGACAGAAGG ACAGTCTGTGTCCATATTTGGACCAGGAACACCAAGATGGTGTGATGGTGGCCACCATGCACATGAAGACAATGACA GCAATGAAACTCAAATAGAGACTGAGGGAACATCTATGTCCGTGTTTGGGCCAGGAACACCAAACAAGCAGTTTACA ACTCAAGACTTGAACAGTACCAAACACCCAGAATCCCA (A types) or primer pair S6 are amplified AGGTCTCACAGTCTGCACAACACCTCATACACACACTCACACATTTAAACACAGATGCACTCCTGCACACATACATG CACATAAAAACTCACACATGCAAATCTATTCCAACACTGCAGACTAAACACAAACACAAGTTGATGCATCTATATCT TTGGCACAGATGATAAATTAATGGTCCCAAGAGATACCAAATCTCTTATTAGTGTG TTTTGGAGCCTGA (c-type) or Primer pair S7 amplifies TCACCGACTTTACTGCATACATTAGCTGAATTTGAACATCCCATTTGTGTTGTGTT GTCAATA TATTCAACTTATGGCTCGTCACACAAGTTTTTTCTCATGCAAGATTGTGTATTTGTACTTTTTGTGACTTTGTCTAT AAACATTTAGTAAACAGCAGAATTTTTTTTTAATAAAATTATGTGTGAAATAGTTTTGTTAGCATATTTCTTCTGCT CCGTAAGTCTG (T-type) or primer pair S11 amplify CTCTAATGCGACTGGGACGTACAAAACTATCGCATCTAAAGCAC ACTCAGAACTTCACTGTTATCAGATACAATGCAAGTACTTTTCAATCAGTGATGAAACAAACTATATCCGACTTGGT TGTTTTCATCACTTTTACTCCACTGGAACCTTCCTGAAGAAACAGTCTGTAGATGATGGGACTTGCTGCAGTGGCAA CTGAAAGTTTAGCTGTGAAGAATGTGAGGCC (A types) or primer pair S12 amplify GCACTCCCTCAATAAATACACCAT TAAATATTTCCCATATATATTTCCAATATATTAAAAGAAAAAACTAAACAAAACACATTTAAATAGGACAACAAAAT ACAAGTTGGCTCAATAAAAGAAGAAAAAGATACGTGTTATGATTTTAAAACAACTTTTTTTCTCTCTCTCTTACAAT TAGACTTCTTAATCTAACATTGTTAAGGATATTGTTGTCAGGCATTAGTCAAGCA (T-type) or primer pair S13 are amplified TGTTCATTTGCTTTGGACTGATCGTCATGAGCAGTCAGAAGAAAACACAGAAAAATACTGGCCCATATGGTGCTCTT CTGATACTTCTCGGATTAAGTCACGTTTGGCAAGTCCCGGCTTGTGATGTTACAGAGAGCTGTCGGTCGTGCTCTGA GTTTTCTCCTCTGTACTTGAACAGCTCAACAGAGGGCAATAGAGAGGCGAGCGACAGCAGGGCATGATATGGGCAAG CCTCCTCGACAGCTCCTGGAAGACGTTCTTAAAAGTTCCTCTAAGTGCTGAAGAAGAGAAGTAGAATATGAATGGAT CGAGGCACGCATTGAACGTGCTGGTGAGCAGCGCAAACACTCGCCAGTCGGGGCTGTAATTGCCTACATATCCAACC The homozygote of ACGTGTGAGACGTTATAGGGCATGAAGCAGATAATGAAGACCAGAAGCGTG (T-type) turns as high feed The individual of rate.
The individual for the high feed conversion rate that the present embodiment is selected improves 4.95% than remaining individual feed conversion rate~ 17.56%, averagely improve 9.07%.Feed efficiency measurement result is as shown in table 1.
Table 1
Embodiment 2
Improve the selection and breeding of carp feed efficiency：
One, carp selection and breeding basic population is established：
According to build, squamation isophenous character determination 3+288 tail of age mirror carp parent individual composition selection basic population；
Two, basic population carp genomic DNA is extracted：
Individual implantation electronic marker, and isozyme sample is acquired, with tissue DNA kit (Tiangeng biology) extraction gene Group DNA；
Three, the genomic DNA of extraction is diluted to 50ng/ μ L as template, PCR amplification is carried out with primer pair；
Wherein, the upstream primer sequence of primer pair S2 is 5 '-GGAGGAGAGCGATCAAGACA-3 ', downstream primer sequence For 5 '-TGGGATTCTGGGTGTTTGGT-3 '；The upstream primer sequence of primer pair S6 is 5 '-AGGTCTCACAGTCTGCACAA- 3 ', downstream primer sequence 5 '-TCAGGCTCCAAAACACACTAAT-3 '；The upstream primer sequence of primer pair S7 is 5 '- TCACCGACTTTACTGCATACA-3 ', downstream primer sequence 5 '-CAGACTTACGGAGCAGAAGAA-3 '；Primer pair S11 Upstream primer sequence be 5 '-CTCTAATGCGACTGGGACGT-3 ', downstream primer sequence 5 '- GGCCTCACATTCTTCACAGC-3'；The upstream primer sequence of primer pair S12 is 5 '-GCACTCCCTCAATAAATACACCA- 3 ', downstream primer sequence 5 '-TGCTTGACTAATGCCTGACA-3 '；The upstream primer sequence of primer pair S13 is 5 '- TGTTCATTTGCTTTGGACTG-3 ', downstream primer sequence 5 '-CACGCTTCTGGTCTTCATTATCTG-3 '；
15 μ L of PCR reaction systems, wherein 1 μ L of DNA profiling, 10 × buffer, 1.5 μ L, MgCl2(25mmol/L)1.5μL、 0.3 μ L of dNTP (10mmol/L), 0.25 μ L of sense primer (10umol/L), 0.25 μ L of downstream primer (10umol/L), Taq enzyme (5U/ μ L) 0.25 μ L, aseptic deionized water are supplemented to 15 μ L；
PCR response procedures are 94 DEG C of denaturation 3min；94 DEG C of denaturation 15s, 56 DEG C of annealing 15s, 72 DEG C of extension 30s, 35 are followed Ring；Last 72 DEG C of extensions 3min；
Four, selection primer pair S2 amplifies GGAGGAGAGCGATCAAGACAGTGATGTGACTCAAATAGAGACAGAAGG ACAGTCTGTGTCCATATTTGGACCAGGAACACCAAGATGGTGTGATGGTGGCCACCATGCACATGAAGACAATGACA GCAATGAAACTCAAATAGAGACTGAGGGAACATCTATGTCCGTGTTTGGGCCAGGAACACCAAACAAGCAGTTTACA ACTCAAGACTTGAACAGTACCAAACACCCAGAATCCCA or primer pair S6 amplify AGGTCTCACAGTCTGCACAACAC CTCATACACACACTCACACATTTAAACACAGATGCACTCCTGCACACATACATGCACATAAAAACTCACACATGCAA ATCTATTCCAACACTGCAGACTAAACACAAACACAAGTTGATGCATCTATATCTTTGGCACAGATGATAAATTAATG GTCCCAAGAGATACCAAATCTCTTATTAGTGTGTTTTGGAGCCTGA or primer pair S7 are amplified TCACCGACTTTACTGCATACATTAGCTGAATTTGAACATCCCATTTGTGTTGTGTTGTCAATATATTCAACTTATGG CTCGTCACACAAGTTTTTTCTCATGCAAGATTGTGTATTTGTACTTTTTGTGACTTTGTCTATAAACATTTAGTAAA CAGCAGAATTTTTTTTTAATAAAATTATGTGTGAAATAGTTTTGTTAGCATATTTC TTCTGCTCCGTAAGTCTG or Primer pair S11 amplifies CTCTAATGCGACTGGGACGTACAAAACTATCGCATCTAAAGCACACTCAGAACTTC ACTGTT ATCAGATACAATGCAAGTACTTTTCAATCAGTGATGAAACAAACTATATCCGACTTGGTTGTTTTCATCACTTTTAC TCCACTGGAACCTTCCTGAAGAAACAGTCTGTAGATGATGGGACTTGCTGCAGTGGCAACTGAAAGTTTAGCTGTGA AGAATGTGAGGCC or primer pair S12 amplify GCACTCCCTCAATAAATACACCATTAAATATTTCCCATATATATTTC CAATATATTAAAAGAAAAAACTAAACAAAACACATTTAAATAGGACAACAAAATACAAGTTGGCTCAATAAAAGAAG AAAAAGATACGTGTTATGATTTTAAAACAACTTTTTTTCTCTCTCTCTTACAATTAGACTTCTTAATCTAACATTGT TAAGGATATTGTTGTCAGGCATTAGTCAAGCA or primer pair S13 amplify TGTTCATTTGCTTTGGACTGATCGTCAT GAGCAGTCAGAAGAAAACACAGAAAAATACTGGCCCATATGGTGCTCTTCTGATACTTCTCGGATTAAGTCACGTTT GGCAAGTCCCGGCTTGTGATGTTACAGAGAGCTGTCGGTCGTGCTCTGAGTTTTCTCCTCTGTACTTGAACAGCTCA ACAGAGGGCAATAGAGAGGCGAGCGACAGCAGGGCATGATATGGGCAAGCCTCCTCGACAGCTCCTGGAAGACGTTC TTAAAAGTTCCTCTAAGTGCTGAAGAAGAGAAGTAGAATATGAATGGATCGAGGCACGCATTGAACGTGCTGGTGAG CAGCGCAAACACTCGCCAGTCGGGGCTGTAATTGCCTACATATCCAACCACGTGTGAGACGTTATAGGGCATGAAGC The homozygote parent of AGATAATGAAGACCAGAAGCGTG is as parental population；
Five, parental population combo is bred.
The present embodiment parental population totally 115 tails (wherein 64 tails it is amplifiable go out 3 step 4 recorded in sequence；40 tails can expand Increase and sequence recorded in 4 step 4；11 tails it is amplifiable go out 5 step 4 recorded in sequence).
The present embodiment selection at least amplifies parent's combo breeding of sequence recorded in 3 step 4, and to be free of step The individual (288-115) of sequence recorded in four group as a contrast, breeds filial generation respectively.Same pond condition, the same number of cultivation F1 generation seed is measured, Autoamtic bait putting machine timing fixed point, quantitatively feeds, periodic measurement, and records food consumption.By 150 days support Cultivation is grown, 1000 tails is selected at random for every group and measures, the offspring for the individual reproduction that the method for the present invention is selected has higher Feed efficiency, and accuracy is up to 100%.Moreover, the offspring's body for the individual reproduction that the method for the present invention is selected Weight is also significantly greater than control population filial generation, and increasing degree average value is 12.35%.
Sequence table
<110>Heilongjiang Inst. of Aquatic Products, Chinese Academy of Aquatic Products Scie
<120>A kind of selection improving carp feed efficiency
<150> 2018107189046
<151> 2018-07-03
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catatttgga ccaggaacac caagatggtg tgatggtggc caccatgcac atgaagacaa 120
tgacagcaat gaaactcaaa tagagactga gggaacatct atgtccgtgt ttgggccagg 180
aacaccaaac aagcagttta caactcaaga cttgaacagt accaaacacc cagaatccca 240
<210> 2
<211> 223
<212> DNA
<213>Carp (Cyprinus carpio)
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aggtctcaca gtctgcacaa cacctcatac acacactcac acatttaaac acagatgcac 60
tcctgcacac atacatgcac ataaaaactc acacatgcaa atctattcca acactgcaga 120
ctaaacacaa acacaagttg atgcatctat atctttggca cagatgataa attaatggtc 180
ccaagagata ccaaatctct tattagtgtg ttttggagcc tga 223
<210> 3
<211> 228
<212> DNA
<213>Carp (Cyprinus carpio)
<400> 3
tcaccgactt tactgcatac attagctgaa tttgaacatc ccatttgtgt tgtgttgtca 60
atatattcaa cttatggctc gtcacacaag ttttttctca tgcaagattg tgtatttgta 120
ctttttgtga ctttgtctat aaacatttag taaacagcag aatttttttt taataaaatt 180
atgtgtgaaa tagttttgtt agcatatttc ttctgctccg taagtctg 228
<210> 4
<211> 229
<212> DNA
<213>Carp (Cyprinus carpio)
<400> 4
ctctaatgcg actgggacgt acaaaactat cgcatctaaa gcacactcag aacttcactg 60
ttatcagata caatgcaagt acttttcaat cagtgatgaa acaaactata tccgacttgg 120
ttgttttcat cacttttact ccactggaac cttcctgaag aaacagtctg tagatgatgg 180
gacttgctgc agtggcaact gaaagtttag ctgtgaagaa tgtgaggcc 229
<210> 5
<211> 233
<212> DNA
<213>Carp (Cyprinus carpio)
<400> 5
gcactccctc aataaataca ccattaaata tttcccatat atatttccaa tatattaaaa 60
gaaaaaacta aacaaaacac atttaaatag gacaacaaaa tacaagttgg ctcaataaaa 120
gaagaaaaag atacgtgtta tgattttaaa acaacttttt ttctctctct cttacaatta 180
gacttcttaa tctaacattg ttaaggatat tgttgtcagg cattagtcaa gca 233
<210> 6
<211> 436
<212> DNA
<213>Carp (Cyprinus carpio)
<400> 6
tgttcatttg ctttggactg atcgtcatga gcagtcagaa gaaaacacag aaaaatactg 60
gcccatatgg tgctcttctg atacttctcg gattaagtca cgtttggcaa gtcccggctt 120
gtgatgttac agagagctgt cggtcgtgct ctgagttttc tcctctgtac ttgaacagct 180
caacagaggg caatagagag gcgagcgaca gcagggcatg atatgggcaa gcctcctcga 240
cagctcctgg aagacgttct taaaagttcc tctaagtgct gaagaagaga agtagaatat 300
gaatggatcg aggcacgcat tgaacgtgct ggtgagcagc gcaaacactc gccagtcggg 360
gctgtaattg cctacatatc caaccacgtg tgagacgtta tagggcatga agcagataat 420
gaagaccaga agcgtg 436

Claims (3)

1. a kind of selection improving carp feed efficiency, it is characterised in that the selection carries out according to the following steps：
One, carp selection and breeding basic population is established；
Two, basic population carp genomic DNA is extracted；
Three, using the genomic DNA of extraction as template, PCR amplification is carried out with primer pair；Wherein, the sense primer sequence of primer pair S2 It is classified as 5 '-GGAGGAGAGCGATCAAGACA-3 ', downstream primer sequence 5 '-TGGGATTCTGGGTGTTTGGT-3 '；Primer Upstream primer sequence to S6 is 5 '-AGGTCTCACAGTCTGCACAA-3 ', downstream primer sequence 5 '- TCAGGCTCCAAAACACACTAAT-3'；The upstream primer sequence of primer pair S7 is 5 '-TCACCGACTTTACTGCATACA- 3 ', downstream primer sequence 5 '-CAGACTTACGGAGCAGAAGAA-3 '；The upstream primer sequence of primer pair S11 is 5 '- CTCTAATGCGACTGGGACGT-3 ', downstream primer sequence 5 '-GGCCTCACATTCTTCACAGC-3 '；Primer pair S12's Upstream primer sequence is 5 '-GCACTCCCTCAATAAATACACCA-3 ', downstream primer sequence 5 '- TGCTTGACTAATGCCTGACA-3'；The upstream primer sequence of primer pair S13 is 5 '-TGTTCATTTGCTTTGGACTG-3 ', Downstream primer sequence is 5 '-CACGCTTCTGGTCTTCATTATCTG-3 '；
Four, selection primer pair S2 amplifies GGAGGAGAGCGATCAAGACAGTGATGTGACTCAAATAGAGACAGAAGGACAG TCTGTGTCCATATTTGGACCAGGAACACCAAGATGGTGTGATGGTGGCCACCATGCACATGAAGACAATGACAGCAA TGAAACTCAAATAGAGACTGAGGGAACATCTATGTCCGTGTTTGGGCCAGGAACACCAAACAAGCAGTTTACAACTC AAGACTTGAACAGTACCAAACACCCAGAATCCCA or primer pair S6 amplify AGGTCTCACAGTCTGCACAACACCTCA TACACACACTCACACATTTAAACACAGATGCACTCCTGCACACATACATGCACATAAAAACTCACACATGCAAATCT ATTCCAACACTGCAGACTAAACACAAACACAAGTTGATGCATCTATATCTTTGGCACAGATGATAAATTAATGGTCC CAAGAGATACCAAATCTCTTATTAGTGTGTTTTGGAGCCTGA or primer pair S7 are amplified TCACCGACTTTACTGCATACATTAGCTGAATTTGAACATCCCATTTGTGTTGTGTTGTCAATATATTCAACTTATGG CTCGTCACACAAGTTTTTTCTCATGCAAGATTGTGTATTTGTACTTTTTGTGACTTTGTCTATAAACATTTAGTAAA CAGCAGAATTTTTTTTTAATAAAATTATGTGTGAAATAGTTTTGTTAGCATATTTC TTCTGCTCCGTAAGTCTG or Primer pair S11 amplifies CTCTAATGCGACTGGGACGTACAAAACTATCGCATCTAAAGCACACTCAGAACTTC ACTGTT ATCAGATACAATGCAAGTACTTTTCAATCAGTGATGAAACAAACTATATCCGACTTGGTTGTTTTCATCACTTTTAC TCCACTGGAACCTTCCTGAAGAAACAGTCTGTAGATGATGGGACTTGCTGCAGTGGCAACTGAAAGTTTAGCTGTGA AGAATGTGAGGCC or primer pair S12 amplify GCACTCCCTCAATAAATACACCATTAAATATTTCCCATATATATTTC CAATATATTAAAAGAAAAAACTAAACAAAACACATTTAAATAGGACAACAAAATACAAGTTGGCTCAATAAAAGAAG AAAAAGATACGTGTTATGATTTTAAAACAACTTTTTTTCTCTCTCTCTTACAATTAGACTTCTTAATCTAACATTGT TAAGGATATTGTTGTCAGGCATTAGTCAAGCA or primer pair S13 amplify TGTTCATTTGCTTTGGACTGATCGTCAT GAGCAGTCAGAAGAAAACACAGAAAAATACTGGCCCATATGGTGCTCTTCTGATACTTCTCGGATTAAGTCACGTTT GGCAAGTCCCGGCTTGTGATGTTACAGAGAGCTGTCGGTCGTGCTCTGAGTTTTCTCCTCTGTACTTGAACAGCTCA ACAGAGGGCAATAGAGAGGCGAGCGACAGCAGGGCATGATATGGGCAAGCCTCCTCGACAGCTCCTGGAAGACGTTC TTAAAAGTTCCTCTAAGTGCTGAAGAAGAGAAGTAGAATATGAATGGATCGAGGCACGCATTGAACGTGCTGGTGAG CAGCGCAAACACTCGCCAGTCGGGGCTGTAATTGCCTACATATCCAACCACGTGTGAGACGTTATAGGGCATGAAGC The homozygote parent of AGATAATGAAGACCAGAAGCGTG is as parental population；
Five, parental population combo is bred, and selection and breeding are completed in filial generation sexal maturity.
2. the selection according to claim 1 for improving carp feed efficiency, it is characterised in that step 1 is according to table Type character establishes carp selection and breeding basic population.
3. the selection according to claim 1 for improving carp feed efficiency, it is characterised in that step 2 acquires base Plinth group carp isozyme simultaneously extracts genomic DNA.
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