CN108782615A - One kind killing thrips plant essential oil composition, preparation and its application - Google Patents
One kind killing thrips plant essential oil composition, preparation and its application Download PDFInfo
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- CN108782615A CN108782615A CN201810319827.7A CN201810319827A CN108782615A CN 108782615 A CN108782615 A CN 108782615A CN 201810319827 A CN201810319827 A CN 201810319827A CN 108782615 A CN108782615 A CN 108782615A
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- Prior art keywords
- thrips
- essential oil
- preparation
- wintergreen
- oil composition
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
Abstract
The invention discloses one kind killing thrips plant essential oil composition, preparation and its application, and containing wintergreen and origanum oil, and the weight ratio of wintergreen and origanum oil is 1:6~6:1.The present composition can prevent thrips, and there is apparent synergistic effect, it is not likely to produce drug resistance, while crop immune system ability can be improved, and its vigor can be significantly improved, it is a kind of pollution-free plant source thrips prevention and control agent to safety of human and livestock, environmentally safe.
Description
Technical field
It is specifically a kind of to kill the combination of thrips plants essential oil the present invention relates to a kind of preparation method of technical field of pesticide
Object, preparation and its application.
Background technology
Thrips is the general designation of Thysanoptera (Thysanoptera) Thripidae (Thripida) insect, and individual is small, invisible
By force, reproduction speed is fast.So far, the Thripidae type of whole world record is planted up to more than 2000, is under the jurisdiction of the category of 4 subfamilies about 276, in
State accounts for 315 kinds of 79 category.According to incompletely statistics, thrips pest accounts for about the 1% of existing record kind, based on phytophagous thrips, utilizes
Sucking mouth parts draws the needs that the nutriment in histoorgan meets own growth and development, and passes through direct feeding, oviposition
Or transmitted virus caused harm and caused by passing poison and causing harm loss considerably beyond directly causing harm.Thrips is mainly caused harm a variety of grains
Food, fruits and vegetables, flowers and gardening etc..In recent years, the generation and diffusion of thrips aggravate year by year, it has also become worldwide important agriculture and forestry injurious insect
One of, huge economic loss is caused to agricultural production practice.
Currently, chemical agent is depended on to the prevention of thrips both at home and abroad, however, resistance problems are on the rise, and
Increase the pressure to environment.Botanical pesticide because its is environmental-friendly, to non-target organism safely, to be not likely to produce drug resistance etc. excellent
Point, and the hot spot with application is researched and developed as novel pesticide.
Invention content
The first purpose of the invention is to provide one kind killing thrips plant essential oil composition, the pesticide of wintergreen and origanum oil
It is apparent to the control effect of thrips with synergistic function, it is not likely to produce drug resistance, while crop immune system can be improved
Ability, and its vigor can be significantly improved.
Second object of the present invention is to provide one kind and killing thrips plants essential oil preparation, has efficient noresidue, to non-target
Mark bio-safety is not likely to produce drug resistance, is environmentally friendly.
Third object of the present invention is to provide a kind of applications for killing thrips plant essential oil composition.
The present invention is achieved by the following technical solutions:
First purpose of the present invention is deferred to, one kind killing thrips plant essential oil composition, which includes Chinese ilex
Oil and origanum oil.
Optionally, the weight ratio of the wintergreen and origanum oil is 1:6~6:1.
Optionally, the weight ratio of the wintergreen and origanum oil is 1:1.
Optionally, the weight ratio of the wintergreen and origanum oil is 2:1.
Second object of the present invention is deferred to, one kind killing thrips plants essential oil preparation, and the active ingredient of said preparation is this hair
Thrips plant essential oil composition is killed described in bright.
Optionally, it is described kill mass percentage of the thrips plant essential oil composition in the preparation be 2%~
90%.
Optionally, it is described kill mass percentage of the thrips plant essential oil composition in the preparation be 5%~
80%.
Optionally, the dosage form of the preparation is selected from microemulsion, soluble liquid and thermal fog, at least contains table in preparation
Face activating agent, mass percentage of the surfactant in the preparation are 2%~30%.
Third object of the present invention is deferred to, plant essential oil composition of the present invention is used to prepare prevention grain, fruit
The application of vegetable, flowers and/or gardening plant thrips drug.
Optionally, the thrips is that maize stays thrips, Frankliniella occidentalis and/or onion thrips.
The present invention has the following advantages compared with the prior art:
(1) pesticide containing wintergreen and origanum oil of the invention has synergistic function, bright to the control effect of thrips
It is aobvious, it is not likely to produce drug resistance, while crop immune system ability can be improved, and its vigor can be significantly improved.
(2) raw material natural resources extremely enrich, process it is simple, low for equipment requirements.
(3) this kills thrips agent main component and derives from natural traditional Chinese medicine plant extracts, so its is environmentally safe, it is right
People, animal safety, is a kind of pollution-free plant source thrips prevention and control agent.
Specific implementation mode
Wintergreen is a kind of plants essential oil extracted from leaf of Chinese ilex, and main component is gaultherolin, and content reaches
99% or more.Gaultherolin is the pheromones of plant, and plant can resist attack of insect with it.Since wintergreen has centainly
Restraining and sterilizing bacteria, antipyretic-antalgic and other effects have poisoning and repellent activity well to various pests, have and be developed into environment friend
The potentiality of good type pesticide.
Origanum oil is the plants essential oil extracted from origanum herb, contains more than 30 kinds of antimicrobial compound, is a kind of latent
Natural antibiotics and drug growth promoter, it is significant in poultry and livestock feed, feeding antibiotic, immune etc..Profit
Oil is planted with plant, and one of the hot spot that prevention is current pesticide research is carried out to agriculture diseases, insect pests and weeds.
The content of active constituent depends on amount of application when essential oil is used alone in the present composition, also depends on Chinese ilex
The mixed ratio and synergistic effect degree of oil and origanum oil, at the same it is also related with thrips.Active constituent in usual composition
Weight percentage is the 2%~90% of total weight, preferably 5%~80%.According to different preparation types, active constituent
Content range is different.
At least contain a kind of surfactant and solvent in the dosage form of the present invention, it can be with according to different dosage forms, in preparation
Containing stabilizer known in those skilled in the art, antifreeze etc., remaining is by water completion.
Surface-active contents are the 2%~30% of total formulation weight, may be used nonionic surfactant or it is cloudy from
At least one of sub- surfactant.Nonionic surfactant can be styrenated phenol polyoxyethylene poly-oxygen propylene aether, three
Styryl phenol polyethenoxy ether, octyl phenol polyoxyethylene ether, nonylphenol polyoxyethylene ether, castor oil ethylene oxide adduct and
At least one of its derivative, polyol fatty acid ester and its ethylene oxide adduct, fatty alcohol polyoxyethylene ether etc..The moon from
Sub- surfactant is calcium dodecyl benzene sulfonate, alkyl phenol polyoxyethylene ether phosphate, phenethyl phenol polyethenoxy ether phosphoric acid
At least one of ester etc..At least one of ethyl alcohol, isopropanol, n-butanol, ethyl acetate, acetone etc. may be used in solvent.
Water can be one kind in distilled water, deionized water, tap water, and more optimized is deionized water.
For example, by mass percentage, when microemulsion is made, killing thrips plant essential oil composition 5%~40%, surface is lived
Property agent 5%~50%, auxiliary agent 5%~35%, deionized water add to 100%.
When soluble liquid is made, thrips plant essential oil composition 5%~45%, surfactant 5%~10%, molten is killed
Agent 5%~90%.
When thermal fog is made, kill thrips plant essential oil composition 10%~40%, surfactant 5~35%, solvent 5~
35%, auxiliary agent 5%~20%.
The microemulsion the key technical indexes of the present invention:
The soluble liquid the key technical indexes of the present invention:
The thermal fog the key technical indexes of the present invention:
Synergy is studied:
1, research method:
The method that the present invention is combined using Toxicity Determination and field trial.Toxicity Determination is first passed through, it is clear
Two kinds of medicaments compound by a certain percentage after co-toxicity coefficient (CTC), CTC < 80 be antagonism, 80≤CTC≤120 be added
Effect, CTC > 120 are synergistic effect, on this basis, then carry out field trial.
Test method:Fumigation activity, which measures, uses triangular flask fumigating system.Specific method is:In triangular flask (V=60mL)
The neat test worm of 20 health is accessed, a filter paper item (3*1cm) is then fixed on rubber stopper, microsyringe is used in combination to filter
1 μ L essential oil solution is added dropwise in paper slip, stoppers rubber stopper rapidly, so that medicinal chart piece is just hung at the 1/3 of bottle center, is positioned over intelligence
Incubator (L:D=16:8, T=25 ± 1 DEG C, RH=60 ± 10%), triangle bottleneck is opened after 6h, pulls out the plug with filter paper
Son is observed fumigate lethal cases for 24 hours, in triplicate, acetone control is arranged in each processing afterwards with preservative film by bottle sealing.If
It compares the death rate and is more than 10%, be then considered as invalid experiment, corrected mortality is calculated using Abbott formula.
Calculation formula is as follows:
The death rate (%)=death borer population/total borer population × 100%
Corrected mortality (%)=(the processing death rate-control death rate)/(1- compares the death rate) × 100%
Virulence regression equation is acquired using probit analysis and calculates LC50 values, is calculated altogether according to the abundant equations of Sun Yun
Malicious coefficient CTC.
Calculation formula is following (using wintergreen as standard agent, toxicity index 100):
LC50 × 100 of the LC50/ active constituents B of toxicity index (TI)=wintergreen of active constituent B
LC50 × 100 of the LC50/M of toxicity index (ATI)=wintergreen of M
The theoretical toxicity index (TTI) of M
TI × P active constituents B of TI × P wintergreens+active constituent B of=wintergreen
TTI × 100 of the ATI/M of co-toxicity coefficient (CTC)=M
In formula:M is the mixture of different ratio;B is origanum oil;P active constituents B is active constituent B institutes in the composition
The ratio accounted for;P wintergreens are wintergreen shared ratio in the composition;
2, result of study:
2.1 maize stay thrips synergy research
Test medicine is provided by Ji'an City Zhong Xiang natural plants Co., Ltd of Jiangxi Province.
Experimental design:The effective lethal dense of wintergreen and origanum oil and the two different ratio mixture is determined by pilot study
Spend range.Toxicity test result is as follows:
1 wintergreen of table stays maize with origanum oil compounding the toxicity test analysis of results table of thrips
Reagent agent | Proportioning | LC50 | Co-toxicity coefficient (CTC) |
Wintergreen | - | 2.56 | - |
Origanum oil | - | 3.27 | - |
Wintergreen:Origanum oil | 1:6 | 2.23 | 141.04 |
Wintergreen:Origanum oil | 1:5 | 1.74 | 149.54 |
Wintergreen:Origanum oil | 1:4 | 2.15 | 144.09 |
Wintergreen:Origanum oil | 1:3 | 1.89 | 161.79 |
Wintergreen:Origanum oil | 1:2 | 2.06 | 145.39 |
Wintergreen:Origanum oil | 1:1 | 1.45 | 198.05 |
Wintergreen:Origanum oil | 2:1 | 1.57 | 175.77 |
Wintergreen:Origanum oil | 3:1 | 1.23 | 143.98 |
Wintergreen:Origanum oil | 4:1 | 1.98 | 135.16 |
Wintergreen:Origanum oil | 5:1 | 2.66 | 126.48 |
Wintergreen:Origanum oil | 6:1 | 2.41 | 121.75 |
As shown in Table 1, wintergreen and origanum oil compounding prevention maize stay the proportioning of thrips 1:6~6:When 1, poison is altogether
Number is all higher than 120, illustrates the two 1:6~6:Mixture shows synergistic effect in 1 range, when matching for wintergreen and origanum oil
Than 1:Co-toxicity coefficient is maximum when 1, and synergistic effect is the most apparent.
2.2 Frankliniella occidentalis synergy are studied:
Test medicine is provided by Ji'an City Zhong Xiang natural plants Co., Ltd of Jiangxi Province.
Experimental design:The effective lethal dense of wintergreen and origanum oil and the two different ratio mixture is determined by pilot study
Spend range.Toxicity test result
2 wintergreen of table compounds the toxicity test analysis of results table to Frankliniella occidentalis with origanum oil
Reagent agent | Proportioning | LC50 | Co-toxicity coefficient (CTC) |
Wintergreen | - | 3.02 | - |
Origanum oil | - | 3.78 | - |
Wintergreen:Origanum oil | 1:6 | 3.10 | 121.23 |
Wintergreen:Origanum oil | 1:5 | 3.26 | 130.50 |
Wintergreen:Origanum oil | 1:4 | 2.69 | 133.79 |
Wintergreen:Origanum oil | 1:3 | 2.46 | 146.35 |
Wintergreen:Origanum oil | 1:2 | 2.86 | 147.78 |
Wintergreen:Origanum oil | 1:1 | 2.34 | 152.62 |
Wintergreen:Origanum oil | 2:1 | 3.34 | 161.04 |
Wintergreen:Origanum oil | 3:1 | 2.19 | 145.19 |
Wintergreen:Origanum oil | 4:1 | 3.55 | 135.05 |
Wintergreen:Origanum oil | 5:1 | 3.43 | 127.54 |
Wintergreen:Origanum oil | 6:1 | 3.67 | 124.87 |
As shown in Table 2, the proportioning of wintergreen and origanum oil compounding prevention Frankliniella occidentalis is 1:6~6:When 1, co-toxicity coefficient is equal
More than 120, illustrate the two 1:6~6:Mixture shows synergistic effect in 1 range, when the proportioning of wintergreen and origanum oil exists
2:Co-toxicity coefficient is maximum when 1, and synergistic effect is the most apparent.
2.3 onion thrips synergy are studied:
Test medicine is provided by Ji'an City Zhong Xiang natural plants Co., Ltd of Jiangxi Province.
Experimental design:The effective lethal dense of wintergreen and origanum oil and the two different ratio mixture is determined by pilot study
Spend range.Toxicity test result
3 wintergreen of table compounds the toxicity test analysis of results table to onion thrips with origanum oil
As shown in Table 3, the proportioning of wintergreen and origanum oil compounding prevention Frankliniella occidentalis is 1:6~6:When 1, co-toxicity coefficient is equal
More than 120, illustrate the two 1:6~6:Mixture shows synergistic effect in 1 range, when the proportioning of wintergreen and origanum oil exists
1:Co-toxicity coefficient is maximum when 1, and synergistic effect is the most apparent.
It elaborates below to the embodiment of the present invention, the present embodiment is carried out lower based on the technical solution of the present invention
Implement, gives detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1:Microemulsion
(1) preparation is prepared:When microemulsion is made in plant essential oil composition, component and weight percent are:Plant essence
Fluid composition 5%~40%, surfactant 5%~50%, auxiliary agent 5%~35%, deionized water adds to 100%.Specifically match
System is shown in Table 4.
Table 4 is prepared containing the microemulsion of wintergreen and origanum oil
(2) microemulsion prevents the thrips test of pesticide effectiveness:It is random row that field control effectiveness test, which uses plot experiment, plot experiment,
Row, are repeated 3 times, plot area 1m2.Per cell singling label, 10 have thrips blade, investigate insect population before medicine, 1 after medicine,
3, the thrips number living of investigation in 7 days.Worm amount before and after each chemicals treatment dispenser is recorded, the control effect of each chemicals treatment is calculated.It calculates and rectifys
Positive preventive effect
Calculation formula is as follows:
Control effect=[1- (borer populations of living before the dispenser of check plot
Borer population living after the dispenser of × treatment region)/(borer population of living after the dispenser of check plot
Borer population × 100% living before the dispenser of × treatment region
This experiment is arranged in Xibei Univ. of Agricultural & Forest Science & Technology experimental plot, and test medicine is by fragrant natural plants in Jiangxi Province's Ji'an City
Co., Ltd provides, and comparison medicament is wintergreen microemulsion and origanum oil microemulsion, is autogamy.Maize is investigated before medicine stays Ji
Horse insect pest index 1 day after dispenser, investigates insect pest indexes and calculates preventive effect for 3 days, 7 days in insect pest early period of origination dispenser.
Test result is as follows:
5 microemulsion of table prevents the thrips test of pesticide effectiveness
As can be seen from Table 5, wintergreen has good preventive effect, prevention effect with three kinds of thrips of origanum oil complex microemulsion pair
Fruit is superior to the preventive effect of single dose, and preventive effect phase is longer.It has no adverse effects to target crop in experiment scope of medication.
Embodiment 2:Soluble liquid
(1) preparation is prepared:When soluble liquid is made in plant essential oil composition, component and weight percent are:It plants
Object essential oil composition 5%~45%, surfactant 5%~10%, solvent 5%~90%.Specific prepare is shown in Table 6.
Table 6 is prepared containing the soluble liquid of wintergreen and origanum oil
(2) the thrips test of pesticide effectiveness is prevented:This experiment is arranged in Xibei Univ. of Agricultural & Forest Science & Technology experimental plot, and test medicine is by Jiangxi
It saves Ji'an City Zhong Xiang natural plants Co., Ltd to provide, comparison medicament is wintergreen soluble liquid and origanum oil solubility liquid
Agent is autogamy.Maize is investigated before medicine and stays thrips insect pest index, in insect pest early period of origination dispenser, 1 day, 3 days, 7 days after dispenser
Investigation insect pest index simultaneously calculates preventive effect (computational methods are with embodiment 1).
Test result is as follows:
7 soluble liquid of table prevents the thrips test of pesticide effectiveness
As can be seen from Table 7, wintergreen has good preventive effect with three kinds of thrips of origanum oil compounded soluble liquor pair, prevents
The preventive effect that effect is superior to single dose is controlled, and preventive effect phase is longer.It has no adverse effects to target crop in experiment scope of medication.
Embodiment 3:Thermal fog
(1) preparation is prepared:When thermal fog is made in plant essential oil composition, component and weight percent are:Plant essence
Fluid composition 10%~40%, surfactant 5~35%, solvent 5~35%, auxiliary agent 5%~20%.Specific prepare is shown in Table 8.
Table 8 is prepared containing the thermal fog of wintergreen and origanum oil
(2) thermal fog prevents the thrips test of pesticide effectiveness:This experiment is arranged in Xibei Univ. of Agricultural & Forest Science & Technology experimental plot, test medicine
By Ji'an City Zhong Xiang natural plants Co., Ltd of Jiangxi Province provide, comparison medicament be wintergreen thermal fog and origanum oil thermal fog,
It is autogamy.Maize is investigated before medicine and stays thrips insect pest index, in insect pest early period of origination dispenser, 1 day after dispenser, is adjusted within 3 days, 7 days
It looks into insect pest index and calculates preventive effect (computational methods are with embodiment 1).
Test result is as follows:
9 thermal fog of table prevents the thrips test of pesticide effectiveness
As can be seen from Table 9, wintergreen has good preventive effect, prevention effect with origanum oil compounding three kinds of thrips of thermal fog pair
Fruit is superior to the preventive effect of single dose, and preventive effect phase is longer.It has no adverse effects to target crop in experiment scope of medication.
Claims (10)
1. one kind killing thrips plant essential oil composition, which is characterized in that the essential oil composition includes wintergreen and origanum oil.
2. according to claim 1 kill thrips plant essential oil composition, which is characterized in that the wintergreen and origanum oil
Weight ratio be 1:6~6:1.
3. according to claim 1 kill thrips plant essential oil composition, which is characterized in that the wintergreen and origanum oil
Weight ratio be 1:1.
4. according to claim 1 kill thrips plant essential oil composition, which is characterized in that the wintergreen and origanum oil
Weight ratio be 2:1.
5. one kind killing thrips plants essential oil preparation, which is characterized in that the active ingredient of said preparation is any rights of claim 1-4
It is required that described kills thrips plant essential oil composition.
6. according to claim 5 kill thrips plants essential oil preparation, which is characterized in that described kills thrips plants essential oil group
It is 2%~90% to close mass percentage of the object in the preparation.
7. according to claim 5 kill thrips plants essential oil preparation, which is characterized in that described kills thrips plants essential oil group
It is 5%~80% to close mass percentage of the object in the preparation.
8. according to claim 5 kill thrips plants essential oil preparation, which is characterized in that the dosage form of the preparation is selected from micro-
Emulsion, soluble liquid and thermal fog at least contain surfactant, quality of the surfactant in the preparation in preparation
Percentage composition is 2%~30%.
9. the plant essential oil composition described in claim 1-4 any claims is used to prepare prevention grain, fruits and vegetables, flowers
And/or the application of gardening plant thrips drug.
10. application according to claim 9, which is characterized in that the thrips is that maize stays thrips, Frankliniella occidentalis
And/or onion thrips.
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Cited By (2)
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CN114287450A (en) * | 2022-01-13 | 2022-04-08 | 南宁市农业科学研究所 | Pesticide composition for preventing and treating sugarcane thrips |
WO2022237008A1 (en) * | 2021-05-13 | 2022-11-17 | 中国热带农业科学院环境与植物保护研究所 | Pesticide composition for preventing and treating banana thrips |
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CN102014616A (en) * | 2008-03-19 | 2011-04-13 | 蒂拉德克公司 | Pest control using natural pest control agent blends |
JP2012036097A (en) * | 2010-08-03 | 2012-02-23 | Sumitomo Chemical Co Ltd | Pest-controlling composition |
AU2012320093B2 (en) * | 2011-10-04 | 2017-07-13 | 0903608 B.C. Ltd. | Pest control formulations and methods of making and using same |
CN104041504A (en) * | 2014-05-08 | 2014-09-17 | 中国农业科学院茶叶研究所 | Behavior control agent for control tea tree and leaf empoasca vitis and dendrothrips minowai priesner |
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WO2022237008A1 (en) * | 2021-05-13 | 2022-11-17 | 中国热带农业科学院环境与植物保护研究所 | Pesticide composition for preventing and treating banana thrips |
CN114287450A (en) * | 2022-01-13 | 2022-04-08 | 南宁市农业科学研究所 | Pesticide composition for preventing and treating sugarcane thrips |
CN114287450B (en) * | 2022-01-13 | 2023-02-28 | 南宁市农业科学研究所 | Pesticide composition for preventing and treating sugarcane thrips |
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Application publication date: 20181113 |
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