CN108770679A - A kind of high yield, more anti-, high breeding methods for matching corn inbred line new lines - Google Patents
A kind of high yield, more anti-, high breeding methods for matching corn inbred line new lines Download PDFInfo
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- CN108770679A CN108770679A CN201810497500.9A CN201810497500A CN108770679A CN 108770679 A CN108770679 A CN 108770679A CN 201810497500 A CN201810497500 A CN 201810497500A CN 108770679 A CN108770679 A CN 108770679A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention belongs to corn breeding techniques fields, and in particular to a kind of high yield, more anti-, high breeding methods for matching corn inbred line new lines.A kind of high yield, more anti-, high breeding methods for matching corn inbred line new lines, be by corn strain PS group be selfed by preferred strain, educate to obtain high yield, how anti-, high with corn inbred line new lines SP221.It is an advantage of the invention that as basic breeding material PS groups highly resistance, exterior quality is excellent, robustness is good, Recycling breeding strategy and high density has been used to select stress strategy, the merits such as the SP221 educated has accumulated high yield, more anti-, height are matched can be used for assembling the basic material of superior corn cenospecies and corn germplasm improvement in one.
Description
Technical field
The invention belongs to corn breeding techniques fields, and in particular to a kind of high yield more anti-, high matches corn inbred line new lines
Breeding method.
Background technology
Corn originates from South America, and the incoming China of 1556 Christian eras corn after the cultivation of centuries, has formd abundant
Type and kind.Corn is global first generalized grain crop.China's maize sown area is stablized in 30,000,000 hm in recent years2With
On, have reached 3494.9 ten thousand hm within 20122, total yield reaches 208,120,000 t, is more than that paddy produces, becomes the first generalized grain of China
Crop.Corn is not only grain, forage crop is more important, while being also the raw material of industry and energy-source plant, in national economy
In occupy an important position.
Currently, the existing corn variety in China cannot meet production needs, mainly the not strong, yield including kind resistance
Low, adaptability is not wide, hybrid seed yield is low, seed quality is poor, it is difficult to adapt to the requirement of maize production mechanization.Maize seed industry develops
Falling behind relatively, the situation faced is especially severe, although domestic corn variety quantity is more, original self-mating system level is low, with
Some external Dominant varieties are compared, and most domestic corn variety is not suitable for dense planting and do mechanization operation, and resistance is poor, yield carries
The outstanding problems such as related industry development are much lagged behind at high speed, are weakened China's breeding advantage, are seriously threatened grain security.
Excellent germ plasm resource is selection and breeding high level self-mating system and the necessary condition of cenospecies.It is selected from cenospecies is carried out in China
Since educating, using at most, most preferably Temperate maize germplasm；Breeder includes the Temperate zone germplsam including U.S.'s germplasm using these
Large quantities of excellent corn inbred lines are selected, such as 330, yellow morning four, E28,478, Zheng 58,178,18-599,08-641,698-3
Deng, and group allots a large amount of elite hybrid, pushes China's corn breeding is horizontal constantly to reach a new high；China's corn breeding and
The Germplasms of production are nearly all with temperature that Lancaster, Rui De, tangsipingtou, Lucia Red Cob and PN crowd are representative
Band germplasm.However, over time, the hereditary basis of this only Temperate zone germplsam has been increasingly becoming limitation corn breeding water
The flat bottleneck factor further increased.Guangxi tropical subtropical zone, ecotype is more, and tillage and cultivation condition difference is big,
Corn producing region is mostly mountain and hill, and Rainy sparse sunlight, soil property is barren, and pest and disease damage is serious, is fitted to corn variety diversity, extensively in production
Property require it is high；Utilize Temperate zone germplsam merely, selection and breeding not only high yield but also disease-resistant and wide adaptability kind is increasingly difficult.
Change this passive situation, it is necessary to it utilizes in germ plasm resource and above has breakthrough, since last century the eighties, I
Area payes attention to utilizing Tropical germplsam resource always, using it is most, best be also tropical and semi-tropical population germplasm；Include using these
Tropical germplsam including Thailand, CIMYYT germplasm selects large quantities of excellent corn inbred lines, as F06, M9, double M9, south 99,
S37, S1611, S48, T32, S11 etc., and group allots a large amount of elite hybrid, pushes Southwestern China corn breeding horizontal constantly
It reaches a new high.Torrid areas ecological condition and Temperate Region in China are far different, and tropical corn forms abundant hereditary variation class
Type, wherein being much that Temperate maize is no, honest and Monsanto is exactly mutually improved using tropical and subtropical maize germplasm, is bred as
Honest 619 and Di Ka 007 is planted extensively in Guangxi Mountains.Further to excavate the Exploitative potential of existing corn germplasm and utilizing model
It encloses, emphasis is improved using excellent tropical and subtropical maize germplasm, and recombination choosing is basic population, polymerization high yield, resistance to close, degeneration-resistant excellent
Gene widens tropical and semi-tropical population Germplasm Bases, is passivated the photoperiod sensitivity of Tropical germplsam, puts forth effort to filter out the suitable torrid zone
Subtropical zone plantation high yield height match, the resistance to new self-mating system of close, degeneration-resistant corn, assemble breakthrough cenospecies, be preferably jade
Rice production service.Carry out tropical and semi-tropical population Genetic improvement, innovation for a long time using the unique geography and climate resources advantage in Guangxi
And research on utilization, the high yield of suitable Guangxi plantation, resistance to close, degeneration-resistant, extensively suitable Extension of New Corn Varieties application are selected, to increasing agriculture
The people take in, and promote the development of Guangxi Maize Industry, it is ensured that Guangxi grain security promotes the Continuous Innovation of corn scientific research and improves beautiful
The scientific research level of the rice breeding talent has great importance.
Practice have shown that the embodiment of corn breeding achievement is mainly manifested in the selection and breeding of strong hybrid vigour cenospecies, and strong hybrid
The key of superior hybrid crosses selection and breeding is that high-quality selection and breeding, high yield, more anti-, height are used as the male parent of hybridization, female parent with corn inbred line.
Therefore, it is badly in need of cultivating high yield, more anti-, high corn inbred lines matched.
Being disclosed in the information of the background technology part, it is only intended to increase understanding of the overall background of the invention, without answering
It has been the prior art well known to persons skilled in the art when being considered as recognizing or imply that the information is constituted in any form.
Invention content
In view of the deficiencies of the prior art, the object of the present invention is to provide a kind of high yield, more anti-, height to match corn inbred line new product
The breeding method of system.
Technical solution provided by the invention is：
A kind of high yield, more anti-, high breeding methods for matching corn inbred line new lines, include the following steps：
(1) by analysis of genetic diversity, S313, S8232, S Thailand 41, S Thailands 152, S84-6, S152, NF818- are selected
61, ten genetic distances of SW8211, SP220, SW8241 are close and containing the corn breeding group that gulf blood source material of reviving is set up, through 4
Wheel respectively carries out the resistance of the group, high grain yield and its composition character using hybrid selection in Nanning, Baise, Yizhou
PS groups made of selection improvement；
(2) it utilizes preferred strain of corn strain PS groups through three generations to be selfed, obtains S3 generation strains：
(3) strain for selecting conformability good from S3 generation strains, using Recycling breeding strategy strategy, the strain that will be chosen
Hybridization, then handed in vain by preferred strain from filial generation and educate to obtain corn inbred line new lines SP221.
Preferably, in step (2), selection when being handed in vain using the preferred strain of PS groups of three generations of progress of corn strain is planted
Density is 60000 plants/hm2。
Preferably, carrying out combining ability test to S3 generation strains in step (3), it is good therefrom to select conformability
PS05-2-2-1, PS05-2-2-2 and PS05-2-2-6 strain, using Recycling breeding strategy strategy, then by PS05-2-2-1, PS05-2-
Corn inbred line new lines SP221 educates to obtain through three preferably strain selfings from generation to generation in 2-2 and PS05-2-2-6 test crosses choosing system.
Preferably, in step (3), in the good strain of the selection conformability from S3 generation strains, planting density is selected
For 60000 plants/hm2；When carrying out preferred strain selfing in the strain cross that will be chosen, then after hybridization in generation, selection plantation
Density is 75000 plants/hm2。
Preferably, in step (3), the preferred strain selfing of continuous two generation further is carried out to SP221, stays and selects fruit ear,
Gained seed gives over to breeder's seed.
Compared with prior art, the present invention has the advantages that：
The present invention has selected PS groups as breeding basic material, using Recycling breeding strategy strategy, selects to press in higher density
Under power, is reasonably selected, is measured, is evaluated by conventional selfing, the SP221 for cultivating gained realizes following advantageous effect：
(1) breeding time is suitable：Packing tightly property of bract is good, and when production of hybrid seeds can reduce seed and rot：Grain is deeper, and seed-producing rate is high, favorably
In efficiently using for nutrient：Seed orange colour, Hard grain type, exterior quality are excellent.
(2) yield is higher, and tassel is more flourishing, general yield 3750-5250kg/hm2, yield potentiality 6000kg/hm2：In group
When with corn hybrid seed, male parent can be not only done, but also female parent can be done.
(3) conformability is good.Using SP221, oneself through group allots 6 superior corn cenospecies, wherein oneself authorization of 2 combinations, 2
It is a to be combined into 2018《Guangxi conventional corn new varieties regional testing》, 2 combinations are positive to be participated in 2018《Guangxi conventional corn
Joint rating test》.
(4) comprehensive resistance is good, identified, can high Resistance To Helminthosporium Turcicum (1 grade), highly resistance helminthosporium maydis (1 grade of >, high resistance to bacterial wilt (1
Grade), high Resistant Gene To Rust (1 grade), in drought tolerance and resistance to overhead flooding injury on.
Specific implementation mode
The specific implementation mode of the present invention is described in detail with reference to embodiment, it is to be understood that the guarantor of the present invention
Shield range is not restricted by specific implementation.
Unless otherwise explicitly stated, otherwise in entire disclosure and claims, term " comprising " or its change
It changes such as "comprising" or " including " etc. and will be understood to comprise stated element or component, and do not exclude other members
Part or other component parts.
Corn strain involved in embodiment PS groups, strain PS05-2-2-1, PS05-2-2-2 and PS05-2-2-6
From Guangxi Academy of Agricultural Sciences, corn research institute obtains.
Embodiment 1：The breeding method that corn strain is PS groups
(1) 2003 year spring to DKO08F, OA110, S313, S Meng five, S8232, S Thailand 41, S38M, S Thailand 152, S84-6,
SHY41, S152, NF818-61, SW8211, SP220, SW8241, SD90131, Y708M, F880, elder generation 09121, GO081 etc. 30
Multiple free self-mating systems pass through analysis of genetic diversity；
(2) 2003 year autumn is according to analysis of genetic diversity as a result, selecting S313, S8232, S Thailand 41, S Thailands 152, S84-
6, ten genetic distances such as S152, NF818-61, SW8211, SP220, SW8241 are close and containing gulf blood source material equivalent seed of reviving
Plantation obtains corn breeding group after fully mixed powder hybridization；
(3) 2004 year spring to autumn in 2005 applies hybrid selection to this in Nanning, Baise, Yizhou respectively through 4 wheels
The resistance of group, high grain yield and its composition character carry out made of selection improvement PS groups；In this step, selection plantation
Density is 60000 plants/hm2。
Embodiment 2：The breeding method of corn inbred line new lines SP221
(1) 2006 year, inventor select S313, S8232, S Thailand 41, S Thailands 152, S84-6, S152, NF818-61,
Ten genetic distances such as SW8211, SP220, SW8241 it is close and containing Soviet Union gulf blood source material group and pass through improve obtain corn
Group PS groups.Spring~2007 year spring in 2006 selects excellent strain to be selfed and select to S3 generations, at this with pedigree method from the group
In a step, select planting density for 60000 plants/hm2；
(2) 2007 year autumn year carries out combining ability test to S3 strains, autumn in 2008 therefrom identify PS05-2-2-1,
The strains such as PS05-2-2-2 and PS05-2-2-6 select planting density for 60000 plants/hm in this step2；
(3) 2009 year spring, using Recycling breeding strategy strategy, with PS05-2-2-1, PS05-2-2-2 from the same group
It is for selecting with PS05-2-2-6 mixed pollination composition basic material (SP-2-2-1 × SP-2-2-2 × SP-2-2-6).2009
Autumn year from the choosing be selected in basic population it is excellent involve continuous selfing in a criminal case to S3 generations, carry out within 2013 coordinate force, itself yield, disease-resistant
Property, the identification such as ASI and evaluation, therefrom select the strain SP-2-2-1 that itself yield is high, disease-resistant, coordinate force is high and (be named as
SP221), in the step, select planting density for 75000 plants/hm2。
Continue in year in (4) 2011 year -2013 autumn of autumn that SP221 strains are carried out selecting excellent strain selfing, select and remain fruit ear.2014
Excellent strain and typical plant is selected to be selfed, typical fruit ear is stayed on well side.It selects typical plant to carry out selfing and sister after 2015 to hand over
Alternately, well side stays typical fruit ear to do breeder's seed, in this step, selects planting density for 67500 plants/hm2。
Embodiment 3：Resistance, quality and the utilization power of SP221
(1) resistance of SP221
Plant protection research institute of Guangxi academy of agricultural sciences identifies the resistance of SP221 routinely technology carries out identification method,
Qualification result is as follows：The sick grade of southern rust inoculation is 1 grade, belongs to highly resistance；Leaf blight inoculated identification disease grade is 1 grade, belongs to highly resistance；Stigma
Sick inoculated identification disease grade is 1 grade, belongs to highly resistance；Banded sclerotial blight inoculated identification disease grade is 3 grades, belongs to anti-；Bacterial wilt natural occurrence identification knot
Fruit is diseased plant rate 0%, belongs to highly resistance.2014 and rehydration experiment and the sight of many years of field drought tolerance one by one of seedling stage potting arid in 2015
It examines as a result, in drought tolerance category.
(2) quality of SP221
SP221 seed salmon pink, grain is deeper, Hard grain type, and appearance is good.
(3) utilization power of SP221
SP221 cannot be applied to by assembling cenospecies in production in the upper directly application of production.Corn is being assembled with it
When cenospecies, male parent use can be both done, can also do female use.There is SP221 higher coordinate force up to the present to utilize SP221 groups
6 corn Combination novas are allotted to participate in《Guangxi conventional corn new varieties pilot study》, wherein 2 are combined oneself and are authorized by Guangxi
(entitled SP221 × elder generation 21A is combined, variety name is osmanthus single 162, and authorization number examines palace 2013001 for osmanthus；Combine title
For SP221 × SM981, variety name is osmanthus single 166, and authorization number is examined No. 2015007 beautiful for osmanthus；2 are combined into 2018《Extensively
Western conventional corn new varieties regional testing》(code name of participating in the experiment is the single 552 and Gui Dan 553 in osmanthus, combine entitled SP221 × P701 and
SP221 × BCS11,2 combinations are positive to be participated in 2018《Guangxi conventional corn combines rating test》(application code name be osmanthus S184 and
S185, combination title is respectively SP221 × QN122 and SP221 × G2038).
By the above test data it is found that using PS groups as breeding basic material, using Recycling breeding strategy strategy, higher
Density selects that the SP221 resistance that gained is cultivated under pressure is strong, quality is good, conformability is good, and a kind of high yield more anti-, high is matched
Corn inbred line, excellent character are had benefited from inventor's reasonable selection and breeding PS groups and are used as breeding basic material, educated using cycle
Kind strategy, and apply high density and stress is selected (to select planting density for 75000 plants/hm2), so that advantageous and unfavorable character is filled
Divide expression, is conducive to the selection of beneficial gene type.
The description of the aforementioned specific exemplary embodiment to the present invention is in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can much be changed
And variation.The purpose of selecting and describing the exemplary embodiment is that explaining the specific principle of the present invention and its actually answering
With so that those skilled in the art can realize and utilize the present invention a variety of different exemplary implementation schemes and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (5)
1. a kind of high yield, more anti-, high breeding methods for matching corn inbred line new lines, which is characterized in that include the following steps：
(1) by analysis of genetic diversity, select S313, S8232, S Thailand 41, S Thailands 152, S84-6, S152, NF818-61,
Ten genetic distances of SW8211, SP220, SW8241 are close and containing the corn breeding group that gulf blood source material of reviving is set up, through 4 wheels point
The resistance of the group, high grain yield and its composition character are not selected using hybrid selection in Nanning, Baise, Yizhou
PS made of improvement groups；
(2) it utilizes preferred strain of corn strain PS groups through three generations to be selfed, obtains S3 generation strains：
(3) strain for selecting conformability good from S3 generation strains, it is using Recycling breeding strategy strategy, the strain chosen is miscellaneous
It hands over, then is handed in vain by preferred strain from filial generation and educate to obtain corn inbred line new lines SP221.
2. high yield according to claim 1, more anti-, high breeding methods for matching corn inbred line new lines, which is characterized in that
In step (2), when being handed in vain using the preferred strains of three generations of progress of corn strain PS groups select planting density for 60000 plants/
hm2。
3. high yield according to claim 1, more anti-, high breeding methods for matching corn inbred line new lines, which is characterized in that
Combining ability test is carried out to S3 generation strains in step (3), therefrom selects conformability good PS05-2-2-1, PS05-2-2-2
With PS05-2-2-6 strains, selected using Recycling breeding strategy strategy, then by PS05-2-2-1, PS05-2-2-2 and PS05-2-2-6 test cross
Corn inbred line new lines SP221 educates to obtain through three preferably strain selfings from generation to generation in system.
4. high yield according to claim 1, more anti-, high breeding methods for matching corn inbred line new lines, which is characterized in that
In step (3), in the good strain of the selection conformability from S3 generation strains, select planting density for 60000 plants/hm2；It is inciting somebody to action
The strain cross chosen, then when carrying out preferred strain selfing in generation after hybridization, select planting density for 75000 plants/hm2。
5. high yield according to claim 1, more anti-, high breeding methods for matching corn inbred line new lines, which is characterized in that
In step (3), the preferred strain selfing of continuous two generation further is carried out to SP221, stays and selects fruit ear, gained seed gives over to breeder
Seed.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110592259A (en) * | 2019-10-09 | 2019-12-20 | 广西壮族自治区农业科学院玉米研究所 | Molecular marker for detecting corn southern rust resistant gene RPPS313 and application |
| CN111328705A (en) * | 2020-04-23 | 2020-06-26 | 广西壮族自治区农业科学院玉米研究所 | Method for breeding maize inbred line by utilizing farmer variety |
| CN111837942A (en) * | 2020-08-19 | 2020-10-30 | 江苏沿江地区农业科学研究所 | Valence-by-valence incremental germplasm innovation method for corn resistance yield multi-trait combined improvement |
| CN113080054A (en) * | 2021-04-27 | 2021-07-09 | 河北省农林科学院旱作农业研究所 | Breeding method of corn hybrid |
| CN114303941A (en) * | 2022-01-25 | 2022-04-12 | 泸州金土地种业有限公司 | Breeding method of three-high one-excellent corn inbred line (FQ920) |
| CN116530409A (en) * | 2023-05-22 | 2023-08-04 | 河南省农业科学院植物保护研究所 | Creation method of multi-resistance corn germplasm |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592259A (en) * | 2019-10-09 | 2019-12-20 | 广西壮族自治区农业科学院玉米研究所 | Molecular marker for detecting corn southern rust resistant gene RPPS313 and application |
| CN111328705A (en) * | 2020-04-23 | 2020-06-26 | 广西壮族自治区农业科学院玉米研究所 | Method for breeding maize inbred line by utilizing farmer variety |
| CN111837942A (en) * | 2020-08-19 | 2020-10-30 | 江苏沿江地区农业科学研究所 | Valence-by-valence incremental germplasm innovation method for corn resistance yield multi-trait combined improvement |
| CN113080054A (en) * | 2021-04-27 | 2021-07-09 | 河北省农林科学院旱作农业研究所 | Breeding method of corn hybrid |
| CN113080054B (en) * | 2021-04-27 | 2022-03-25 | 河北省农林科学院旱作农业研究所 | Breeding method of corn hybrid |
| CN114303941A (en) * | 2022-01-25 | 2022-04-12 | 泸州金土地种业有限公司 | Breeding method of three-high one-excellent corn inbred line (FQ920) |
| CN116530409A (en) * | 2023-05-22 | 2023-08-04 | 河南省农业科学院植物保护研究所 | Creation method of multi-resistance corn germplasm |
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