CN108739539A - Reduce the aquaculture management method that industrial aquaculture flounder sole anophthalmia side melanism occurs - Google Patents
Reduce the aquaculture management method that industrial aquaculture flounder sole anophthalmia side melanism occurs Download PDFInfo
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- CN108739539A CN108739539A CN201810580193.0A CN201810580193A CN108739539A CN 108739539 A CN108739539 A CN 108739539A CN 201810580193 A CN201810580193 A CN 201810580193A CN 108739539 A CN108739539 A CN 108739539A
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- Prior art keywords
- aquaculture
- flounder
- melanism
- anophthalmia side
- anophthalmia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention relates to the aquaculture management methods that a kind of reduction industrial aquaculture flounder sole anophthalmia side melanism occurs, belong to field of marine aquaculture, left-eyed flounder under the conditions of artificial feeding is different from natural environment, individual is in overfeeding, partial eclipse, environmental pressure and frequent stress situation, thus the ratio of anophthalmia side melanism is very high.A large number of experiments show that wheel animalcule in bait, the type of artemia and state, temperature of cultivation, rate of water exchange and stocking density etc. can all induce the multiple of left-eyed flounder anophthalmia side melanism.The present invention is after the factor for combining above-mentioned influence flounder flounder class anophthalmia side melanism, a kind of reduction industrial aquaculture flounder sole anophthalmia side aquaculture management method that melanism occurs is provided, mainly the ratio for reducing flounder sole seed anophthalmia side melanism and occurring is realized by controlling the ratio of polyunsaturated fatty acid in feed of ingesting, juvenile fish cultivation temperature and density and reducing stress reaction, to improve the commodity value of flounder sole, improve flounder sole productivity effect.
Description
Technical field
The invention belongs to aquatic product health cultivation fields, are sent out more particularly to industrial aquaculture flounder sole anophthalmia side melanism is reduced
Raw aquaculture management method.
Background technology
Left-eyed flounder Malpigmentation phenomenon always exists for a long time.According to the market survey result in eight counties blueness Sen Deng of Japan,
Melanism individual averagely accounts for 6.1% in natural lefteye flounder, occurrence rate and pattern occurs and has difference because of sea area.Under the conditions of artificial feeding
Left-eyed flounder it is different from natural environment, individual is in overfeeding, partial eclipse, environmental pressure and frequent stress situation, thus anophthalmia side
The ratio higher of melanism, many areas are already higher than 80% or more.A large number of experiments show that wheel animalcule in bait, artemia type and
State, temperature of cultivation, rate of water exchange and stocking density etc. can all induce the multiple of left-eyed flounder anophthalmia side melanism.
Newest to study the moieties mechanism for having verified melanism, retinoic acid and its coherent signal regulatory pathway are black
Change the key occurred.Three kinds of nuclear receptor RXR (RXR α, RXR β, RXR γ) of RETINOIC ACID and three kinds of all-trans retinoic acid
Nuclear receptor RAR (RAR α, RAR β, RAR γ) needs and TR (Thyroid Hormone Receptors), VDR (vit D3 receptor) or PPAR (mistakes
Peroxisome proliferator activated receptor) etc. formed heterodimer, in conjunction with target gene retinyl Schiff base salt (RARE) play
Effect, therefore, thyroid hormone etc. can influence retinoic acid to the regulation and control of target gene to influence left-eyed flounder body colour indirectly.Water
Temperature, prelarva stocking density etc., which may be by influence adrenaline, to be influenced thyroid hormones level indirectly and causes Malpigmentation ratio
Example increases.And the type and state of wheel animalcule and artemia may relate to the intake of polyunsaturated fatty acid, the metabolite of the latter,
Such as, prostaglandin (PGE2, PGE3) can be combined to influence body colour adjustment signal access with PPAR γ.Furthermore it is demonstrated that
The sanding feeding of aquaculture pond bottom, melanism can be reduced, it may be possible to which the conduction of skin of abdomen receptor regards Huang by hormone regulating and controlling
The influence of acid signal access.
The present invention provides a kind of reduction batch production after combining above-mentioned numerous factors for influencing flounder flounder class anophthalmia side melanism
The aquaculture management method that flounder sole anophthalmia side melanism occurs is cultivated, mainly by controlling polyunsaturated fatty acid in feed of ingesting
Ratio, juvenile fish cultivate temperature and density and reduce stress reaction to realize the ratio for reducing flounder sole seed anophthalmia side melanism and occurring
Example.
Invention content
The present invention devises the aquaculture management method that a kind of reduction industrial aquaculture flounder sole anophthalmia side melanism occurs, with drop
The generation of low flounder sole anophthalmia side melanism.
The present invention is realized by following technical scheme：
A kind of nutritional control method for reducing industrial aquaculture flounder sole anophthalmia side melanism and occurring, by controlling feed of ingesting
The ratio of middle polyunsaturated fatty acid, juvenile fish cultivation temperature and density and reduce stress reaction come realize reduce flounder sole seed without
The ratio that eye side melanism occurs.
Further, it ingests in feed, polyunsaturated fatty acid aliphatic acid correlation addition in meeting Ministry of Agriculture's feed is marked
On the basis of standard, the ratio of DHA and EPA should be greater than 2.5 times of ARA or more
Further, young juvenile fish cultivation temperature is not higher than 21 DEG C, and keeps relative constant.
Further, young juvenile fish cultivation density on the basis of ensureing culture efficiency in adjustment pond, i.e. the long < 10cm of body, often
Square meter cultivates 150 tails of mantissa <；The long < 15cm of 10cm < bodies cultivate 80 tails of mantissa < per square meter；The long < 20cm of 15cm < bodies, often
Square meter cultivates 50 tails of mantissa <；20cm < bodies are long, and 30 tails of mantissa < are cultivated per square meter；
Further, the stress reaction frequency of the young juvenile fish of cultivation is reduced, sieve seedling takes the mode that nature sorts；Chi Qianyao
Stop eating and anaesthetize, fed at regular time and quantity using automation bait-laying device for fish, reduce unnecessary operation, keeps workshop quiet.
Further, in aquaculture pond bottom sand bedding, the middle grain size that grain size is 0.35-0.6mm is husky, thickness 2-5cm, periodically
Wash husky update.
The technical advantage of the present invention compared with prior art is：
1, present invention firstly provides Synthetical prevention flounder sole anophthalmia side melanism aquaculture management methods
2, method proposed by the present invention, which only needs to control key parameter in daily management, can be realized.
3, the regulation and control method specified of the invention occurs early period regulation flounder sole anophthalmia side melanism meaningful.
Specific implementation mode
Technical scheme of the present invention is further explained below by embodiment, but protection scope of the present invention not by
The limitation of embodiment in any form.
Embodiment 1
2017 we the Tianjin Hangu towns Yang Jiabo prosperity sea water and fresh water cultivation Co., Ltd carried out Cynoglossus semilaevis
Anophthalmia side melanism Synthetical prevention contrast test.Experimental group water temperature is 19-20 DEG C, and dissolved oxygen content is more than 7mg/L, salinity 27%o,
PH is 7.5-7.8, selects original body mass for the Cynoglossus semilaevis of 3.80g ± 0.25g or so, feeds DHA in feed, EPA content is
2.7 times of ARA, cultivation density are：The long < 10cm of body, per square meter, cultivation mantissa is 149-131 tails；The long < 15cm of 10cm < bodies,
Per square meter, cultivation mantissa is 77-65 tails；The long < 20cm of 15cm < bodies, per square meter, cultivation mantissa is 46-41 tails；20cm < bodies are long,
Per square meter, cultivation mantissa is 31-28 tails；Experimental group is 0.4-0.55mm in aquaculture pond bottom sand bedding, grains of sand diameter, and thickness is
4.5cm periodically washes weekly husky update.23-24 DEG C of control group water temperature, dissolved oxygen content are more than 7mg/L, and salinity 27%o, pH are
7.5-7.8 selects original body mass for 4.10g ± 0.22g Cynoglossus semilaevis, feeds DHA in feed, 1.25 that EPA content is ARA
Times, cultivation density is：The long < 10cm of body, per square meter, cultivation mantissa is 221-216 tails；The long < 15cm of 10cm < bodies are supported per square meter
It is 142-127 tails to grow mantissa；The long < 20cm of 15cm < bodies, per square meter, cultivation mantissa is 109-94 tails；20cm < bodies are long, per square meter
Cultivation mantissa is 58-53 tails；Control group aquaculture pond bottom does not process, is smooth cement pit bottom surface.Experimental group and control group are all provided with
Set three parallel, experiments 24 weeks by a definite date.
By the culture experiment of half a year, the results showed that：The experimental group melanism incidence that is averaged is 52.8%, and control group is averaged melanism
Incidence is 86.3%, there were significant differences for the two (p < 0.01)
Therefore reducing Cynoglossus semilaevis anophthalmia side melanism ratio using this method has obvious action, and this method is for reducing factory
The generation for changing flounder sole melanism has important production meaning.
Claims (6)
1. reducing the aquaculture management method that industrial aquaculture flounder sole anophthalmia side melanism occurs, which is characterized in that control feeding of ingesting
The ratio of polyunsaturated fatty acid, juvenile fish cultivation temperature and density and reduction stress reaction reduce flounder sole seed to realize in material
The ratio that anophthalmia side melanism occurs.
2. the aquaculture management method according to claim 1 for reducing industrial aquaculture flounder sole anophthalmia side melanism and occurring,
It is characterized in that ingesting in feed, polyunsaturated fatty acid is on the basis of content of fatty acid standard in meeting Ministry of Agriculture's feed, DHA
It should be greater than 2.5 times or more of ARA with the ratio of EPA
3. the aquaculture management method according to claim 1 for reducing industrial aquaculture flounder sole anophthalmia side melanism and occurring,
It is characterized in that young juvenile fish cultivation temperature is not higher than 21 DEG C, and avoids temperature fluctuation, keep relative constant.
4. the aquaculture management method according to claim 1 for reducing industrial aquaculture flounder sole anophthalmia side melanism and occurring,
It is characterized in that adjusting the young juvenile fish cultivation density in pond on the basis of ensureing culture efficiency, i.e. the long < 10cm of body are cultivated per square meter
150 tails of mantissa <；The long < 15cm of 10cm < bodies cultivate 80 tails of mantissa < per square meter；The long < 20cm of 15cm < bodies are cultivated per square meter
50 tails of mantissa <；20cm < bodies are long, and 30 tails of mantissa < are cultivated per square meter.
5. the aquaculture management method according to claim 1 for reducing industrial aquaculture flounder sole anophthalmia side melanism and occurring,
It is characterized in that reducing the stress reaction frequency of the young juvenile fish of cultivation, sieve seedling takes the mode that nature sorts；Chi Qianyao stop eating and fiber crops
It is liquor-saturated, using feedstuff feeding device at regular time and quantity is automated, unnecessary operation is reduced, keeps workshop quiet.
6. the aquaculture management method according to claim 1 for reducing industrial aquaculture flounder sole anophthalmia side melanism and occurring,
It is characterized in that, in aquaculture pond bottom sand bedding, the middle grain size that grain size is 0.35-0.6mm is husky, and thickness 2-5cm periodically washes husky update.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810580193.0A CN108739539B (en) | 2018-06-07 | 2018-06-07 | Culture management method for reducing occurence of eye-free side blackening of industrial cultured flounder |
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| CN201810580193.0A CN108739539B (en) | 2018-06-07 | 2018-06-07 | Culture management method for reducing occurence of eye-free side blackening of industrial cultured flounder |
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| Publication Number | Publication Date |
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| CN108739539A true CN108739539A (en) | 2018-11-06 |
| CN108739539B CN108739539B (en) | 2020-12-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110024721A (en) * | 2019-04-29 | 2019-07-19 | 中国水产科学研究院北戴河中心实验站 | A method of induction left-eyed flounder ovarian germ cell apoptosis |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144596A (en) * | 2011-05-09 | 2011-08-10 | 中国水产科学研究院黄海水产研究所 | Method for hybridizing female verasper variegatus and male verasper moseri for breeding and fry cultivating |
| CN107410115A (en) * | 2017-09-08 | 2017-12-01 | 山东省海洋生物研究院 | A kind of breeding method for reducing flounder baby fish black and white |
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2018
- 2018-06-07 CN CN201810580193.0A patent/CN108739539B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144596A (en) * | 2011-05-09 | 2011-08-10 | 中国水产科学研究院黄海水产研究所 | Method for hybridizing female verasper variegatus and male verasper moseri for breeding and fry cultivating |
| CN107410115A (en) * | 2017-09-08 | 2017-12-01 | 山东省海洋生物研究院 | A kind of breeding method for reducing flounder baby fish black and white |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110024721A (en) * | 2019-04-29 | 2019-07-19 | 中国水产科学研究院北戴河中心实验站 | A method of induction left-eyed flounder ovarian germ cell apoptosis |
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| CN108739539B (en) | 2020-12-08 |
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