CN108721189A - Have both light spot maintenance, oxidation resistant skin care compositions and its application - Google Patents

Have both light spot maintenance, oxidation resistant skin care compositions and its application Download PDF

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CN108721189A
CN108721189A CN201810929757.7A CN201810929757A CN108721189A CN 108721189 A CN108721189 A CN 108721189A CN 201810929757 A CN201810929757 A CN 201810929757A CN 108721189 A CN108721189 A CN 108721189A
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parts
component
skin care
light spot
care compositions
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CN108721189B (en
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霍昕
陆宽
冯发进
潘年琼
杨迺嘉
杨熟英
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Guizhou Biotechnology Research And Development Base Co ltd
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GUIZHOU PROV BIOLOGICAL TECHN RESEARCH & DEVELOPMENT BASE
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/35—Caprifoliaceae (Honeysuckle family)
    • A61K36/355—Lonicera (honeysuckle)
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/898—Orchidaceae (Orchid family)
    • A61K36/8988—Gastrodia
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
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    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
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    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
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    • A61Q19/00—Preparations for care of the skin
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    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract

The present invention relates to cosmetic technical fields, and in particular to light spot maintenance, oxidation resistant skin care compositions and its application are had both, including：Component A is honeysuckle flower extract, Rhizoma Gastrodiae extract, Ganodenna Lucidum P.E；B component is palmityl tripeptides -1, Argireline；Component C is polyglutamic acid；The present invention is with small molecule bioactive peptide for crease-resistant functional mass, in conjunction with efficient moisture-retention factor polyglutamic acid and the natural Chinese medicinal herb with skin care ingredient carries out balanced collocation, learns from other's strong points to offset one's weaknesses, and the light spot of long-acting moisturizing, maintenance, anti-aging are achieved the effect that on the basis of ensureing safety.

Description

Have both light spot maintenance, oxidation resistant skin care compositions and its application
Technical field
The present invention relates to cosmetic technical fields, and in particular to have both light spot maintenance, oxidation resistant skin care compositions and its Using.
Background technology
Skin aging is the external manifestation of human senility, is since endogenous and the composite factors such as exogenous are coefficient As a result.With advancing age, the aging of skin is more and more obvious, be mainly shown as dry skin, coarse, wrinkle, tarnish, It is nonelastic, pale, loose, or even atrophoderma, crowfoot cracks and senile plaque occur etc..Sunlight or other environmental factors can also draw Play skin aging and impaired, reduction skin activity.The aging of skin not only affects the appearance and image of human body, but also serious The physiological function of human skin is hampered, or even the serious pathology of skin can also be caused to sexually revise, therefore, oxidation resistant skin care item It is concerned.
It is advocated under green, safe and environment-friendly trend along with modern cosmetics, Chinese medicine cosmetic is greatly developed, Matrix summarizes the suitable Chinese medical extract of addition, has both nutrition and the double action of curative effect, also, Chinese herbal medicine convenient material drawing, secondary Act on it is small, therefore, both at home and abroad to add Chinese herbal medicine cosmetics development pay attention to further, the Chinese medicine being selected reach over one hundred kind, but by It is unreasonable in compatibility, be also easy to produce drug resistance, effect slowly, not long-acting, the biocompatibility of other auxiliary materials and middle medicinal substances in addition Difference, and function mismatches so that the toxic side effect of Chinese medicine cosmetic is increasingly serious, greatly limit the development of Chinese medicine cosmetic with It promotes.
Invention content
The present invention is in order to solve the above technical problems, the present invention provides have both light spot maintenance, oxidation resistant skin care compositions And its application.
Specifically realized according to following technical scheme：
Light spot maintenance, oxidation resistant skin care compositions are had both, includes 7-20 parts of component As, 0.1-1.4 parts of B in parts by weight Component, 0.1-3 part component C；The component A is honeysuckle flower extract, Rhizoma Gastrodiae extract, Ganodenna Lucidum P.E；The B component is palm fibre Palmitic acid acyl tripeptides -1, Argireline；The component C is polyglutamic acid.
Further, it is described have both moisturizing, whitening and anti-aging skin care compositions, further include in parts by weight：8-15 parts D components, D groups are divided into cosmetic or cosmetic base.
1. further, the cosmetic includes in parts by weight：32.5-69.7 parts of purified waters, 15-28 parts it is sweet Oil, 0.1-0.3 parts of laruyl alcohol sodium sulfovinates.
Further, the cosmetic base is stearine, cetanol, stearyl alcohol, isopropyl palmitate, poly- two Any one in methylsiloxane, tocopherol, essence, methyl hydroxybenzoate, Nipasol or two or more compositions.
Further, the component A includes in parts by weight：5-10 parts of honeysuckle flower extracts, 1-5 parts of Rhizoma Gastrodiae extracts, 1-5 parts of Ganodenna Lucidum P.Es.
Further, the B component includes in parts by weight：0.05-1.2 parts of palmityl tripeptides -1,0.05-0.2 parts of second Acyl group hexapeptide -8.
The component A, preparation method：Rhizoma Gastrodiae, ganoderma lucidum bulk pharmaceutical chemicals is taken to clean up respectively, it is 77-82 DEG C to be placed in temperature Under the conditions of dry after, slice, obtain gastrodia elata slice, ganoderma lucidum slice；Gastrodia elata slice, ganoderma lucidum slice, honeysuckle flowers are separately added into above-mentioned raw materials medicine weight 10-20 times measure water, impregnate and be no less than 25min, be heated to 90-100 DEG C, decoct 1.0-2.0h, filter to obtain liquid, liquid is passed through For vacuum-concentrcted to 3-5 times of bulk pharmaceutical chemicals weight, filtering takes filtrate respectively as honeysuckle flower extract, Rhizoma Gastrodiae extract, spirit Extract is uniformly mixed to obtain the final product by sesame extract.
It is described have both light spot maintenance, oxidation resistant skin care compositions for facial mask, eye cream, face cream, essence, mildy wash, The production of toner, lotion, hydrosol, sun screen.
Wherein, the production method of any one in essence, toner, hydrosol, facial mask：By cosmetic in D components It is placed in stirring container, is warming up to 65-70 DEG C with the rate of 3-4 DEG C/min, stirring keeps the temperature 5min or more, then with 3-5 DEG C/min Rate be cooled to 35-40 DEG C, component A is added, stirs 10-15min, material is made to be uniformly mixed, be then added essence, B component, Component C stirs 10-15min, discharges, and stands.
Wherein, the production method of any one in face cream, eye cream, mildy wash, lotion, sun screen, includes the following steps：
S1 water phases are pre-dissolved：Glycerine and purified water in D components are placed in the water phase jacketed pan of vacuum emulsifying machine, with 3-4 DEG C/rate of min is warming up to 85-90 DEG C, stirring is opened, after being stirred 5-10min, laruyl alcohol is added in 85 turns/min of rotating speed Sodium sulfovinate is uniformly dispersed；
S2 oil phases are pre-dissolved：By in addition to essence cosmetic base be placed in the oil phase jacketed pan of vacuum emulsifying machine, with 3-4 DEG C/rate of min is warming up to 85-90 DEG C, stirring is opened, 85 turns/min of rotating speed is stirred dissolving；
S3 is emulsified：Water phase, oil phase material are sent into emulsification pot, turn/min, vacuum degree in rotating speed 2500-2800:- 0.03--0.04MPa, the emulsified 5-10min of 70-80 DEG C of condition of temperature are cooled to 35-40 DEG C with the rate of 3-5 DEG C/min, add Enter component A, stir 10-15min, material is made to be uniformly mixed, essence, B component, component C is then added and stirs 10-15min, goes out Material is stood.
Rhizoma Gastrodiae is perennial aulophyte, and return liver warp, mild in medicine property, flavour of a drug are sweet, have effects that suppressing hyperactive liver for calming endogenous wind relieving convulsion, are rich in Gastrodin, gastrodia elata polysaccharide, vitamin A, glucoside, alkaloid, vanillyl alcohol, vanillin, succinic acid etc. can rise in terms of skin care It is supported to skin, is crease-resistant, the effect of Eradicates color spots.
Ganoderma lucidum is the complete stool of Polyporaceae plant red sesame or purple sesame, distributed in lung channel, the heart channel of Hang-Shaoyin, the spleen channel, kidney channel, mild in medicine property, flavour of a drug It is sweet, have effects that strengthening by means of tonics, containing amino acid, polypeptide, protein, fungal lysozyme and carbohydrate (reduced sugar and polysaccharide), Ergosterol, triterpenes, coumarin glucoside, volatile oil, stearic acid, benzoic acid, alkaloid, vitamin etc.；It can in terms of skin care The effects that by delaying senility of humanbody, adjusting endocrine and metabolic system, elimination free radical, achievees the effect that beauty is preserved youthful looks, moreover it is possible to It is enough to keep and adjust moisture of skin, restore skin elasticity, makes skin wet, exquisiteness, and can inhibit the shape of the melanin in skin At and precipitation, remove color spot.
Honeysuckle flowers thoughts of returning home warp, lung channel, pharmacological property is cold, and flavour of a drug are sweet, have effects that clearing heat and detoxicating, dredging collateral, containing inositol, Huang Ketone, inositol, saponin(e, tannin etc..With broad-spectrum antibacterial action, to staphylococcus aureus, hemolytic streptococcus, pneumonia ball The pathogenic bacteria such as bacterium, shigella dysenteriae have stronger inhibiting effect, a variety of to Leptospira, influenza virus and certain dermatophytes etc. Pathogenic microorganism has inhibiting effect.There is apparent anti-inflammatory and refrigeration function.Contained cyanidenon can reach cortex depths, inhibit saturating Bright matter phytase activity inhibits and eliminates the formation of skin splash.And skin blood cycle can be improved, promote skin metabolism, The deposition for inhibiting the activity and melanin of tyrosinase accelerates ecchymose removing decoloration, to play beauty treatment moisturizing, brighten, U.S. of nti-freckle Hold skin effect.
The present invention makes assistant based on Chinese medicine the monarch and his subjects, on the basis of five-element health-preserving, is extracted to effective components in plants, main To include tannin, protein, amino acid, alkaloid, polysaccharide, volatile oil etc., it is made mutually to cooperate with, promote；Wherein, amino acid Not only to the safe of environmental and biological materials, and, irritation is small, and blistering and emulsifiability are good, decontamination energy to skin action temperature Power is strong, and biodegradability is good, also has good bacteriostasis property and bactericidal property.Polysaccharide has film forming, adsorptivity, water Retentivity has excellent lubricity, and moisturizing factor film forming can be helped to be attached to skin surface and form moisture film, effectively remove skin table Face dead cell, deep layer cleaning skin.Protein hydrolyzes to form polypeptide, and peptide has extremely strong activity and diversity, can promote tan Matter, alkaloid, volatile oil isoreactivity ingredient infiltration epidermis are absorbed, and help promotes collagen to generate, and helps anti-inflammatory anti-oxidant. Alkaloid, volatile oil can prevent external environmental or computer from radiating wrinkle, dark and gloomy, dry, shortage gloss caused by skin Deng.Volatile oil can inhibit the generation of tyrosinase activity and active oxygen.
The polyglutamic acid used in the present invention is the main component of viscolloid in natto, contains largely free carboxylic on main chain Base can crosslink, the reactions such as chelating, derivatization, and with strong water-soluble, biocompatibility, biological degradability, have high-strength Water absorbing capacity and slow-release capability, can effectively reduce moisture of skin scatter and disappear, make skin histology elasticity increase, it is soft, have light Pool.Various physiological processes in human body are the polypeptide or protein regulation being nearly all made of specific amino acid sequence.
The palmityl tripeptides -1 that are used in the present invention, Argireline, all have extremely strong activity and diversity, can Tannin, alkaloid, volatile oil isoreactivity ingredient infiltration epidermis is promoted to be absorbed, help promotes collagen to generate, and helps anti-inflammatory It is anti-oxidant.In skin natural aging and nursing process win peptide play an important role, as cell Proliferation, cell migration, inflammation, Pigment is formed and albumen synthesis and regulation and control etc..
The beneficial effects of the present invention are：
The present invention with small molecule bioactive peptide be crease-resistant functional mass, in conjunction with efficient moisture-retention factor polyglutamic acid and tool There is the natural Chinese medicinal herb of skin care ingredient to carry out balanced collocation, learn from other's strong points to offset one's weaknesses, reaches long-acting guarantor on the basis of ensureing safety Wet, the light spot of maintenance, anti-aging effect.
The skin care compositions compatibility science of the present invention can quickly control skin oil spilling, convergence pore, the water for lockking skin Part, while promoting skin metabolism, enhance the vigor and antimicrbial power of skin, enhance Scavenging ability and immunocompetence, Pigmentation is reduced, cutis laxa is prevented.And the present invention skin care compositions be free of western medicine composition, it is non-stimulated to skin safe, Without side-effects, raw material has many advantages, such as to derive from a wealth of sources, at low cost, and has long-acting function.
Currently, existing light spot maintenance, the Chinese medicine of anti-oxidation efficacy of having both is more, such as：, such as：The coptis, Asian puccoon, tree peony Skin etc. has the function of light spot, and dendrobium candidum, heroubill, sea-buckthorn, coix seed etc. have effects that maintenance skin, honeysuckle flowers, Chinese yam, when Return, Rhizoma Chuanxiong etc. have effects that it is anti-oxidant, remove free radical, but be directly aided with cosmetic with Chinese medicine and shield is made in matrix Skin product exist such as the shortcomings of utilization rate of active components is not high, toxic side effect is strong, during being advanced optimized to it, hair The polyglutamic acid for having both high moisture-keeping function and translocation and small molecule active victory peptide, which is now added, can improve traditional Chinese medicine shield The defect of skin product, in order to further promote the light spot maintenance of combination, oxidation resistant performance, we attempt to be added Rhizoma Gastrodiae, ganoderma lucidum, Rhizoma Chuanxiong, the coptis, honeysuckle flowers, Radix Angelicae Sinensis, heroubill etc. more than ten plants medicinal material, carries out the collocation of primary and secondary medication, by multiple recipe determination, It primarily determines using Rhizoma Gastrodiae as monarch drug in a prescription, collocation honeysuckle flowers are ministerial drug, and ganoderma lucidum is assisted, and better effect can be reached.
The present invention is in terms of pigment deposition, anti-oxidant, safety three, to material matching, technique control in the invention System is verified.
One, material matching screens
The present invention from it is numerous have light spot, maintenance, oxidation resistant Chinese medicine material and active ingredient in carry out screening obtain with Under several group of formula (such as table 1)：
Table 1
With above-mentioned composition lotion is produced according to method disclosed by the invention.
1.1 light spot maintenance effects
Melanin genesis in mouse melanin cell is inhibited to test：
By B16 mouse melanin tumor cells with 1 × 105The density of a/ml is connected to the culture dish of a diameter of 9cm, in 37 DEG C ﹑ 5%CO2Under conditions of cultivate 2 days, abandon supernatant, the sample solution 18-22ml after filtration sterilization be added, is then incubated for 3 days, abandons Supernatant, PBS washings, the trypsin solution vitellophag 3min of 0.5ml mass percents 0.2% is added per hole, adds 2ml to tie up per ware Hold liquid terminate digestion piping and druming mixing after, each concentration take out 0.5ml do remaining cell suspension of cell count with 2500r/min from Heart 5min, abandons supernatant, and NaoH solution is added in precipitation, and heating makes melanin dissolve, and selects the enzyme linked immunological of 490nm wavelength Absorbance value is surveyed on detector.
B16 cell inhibiting rate=(1- (drug hole absorbance+drug hole cell density)+(control wells absorbance value+right According to hole cell density)) × 100%.
Melanin genesis in mouse melanin cell is inhibited to test, the results are shown in Table 2
Table 2
Inhibiting rate (%)
Formula group 1 93.7
Formula group 2 82.5
Formula group 3 74.1
Formula group 4 73.9
Formula group 5 70.8
Formula group 6 83.2
Formula group 7 85.5
Formula group 8 88.0
Formula group 9 77.9
Formula group 10 76.5
1.2 anti-oxidation efficacy
Selection DPPH free radical scavenging abilities are tested to test composition oxidation resistance：
The DPPH solution that 0.1mmol/L is prepared with absolute ethyl alcohol, is kept in dark place, test sample solution is configured to a concentration of The solution of 0.5mg/mL, the test sample solution and DPPH solution for drawing 2mL respectively with pipettor are added in same test tube, fill Divide and shake up, is protected from light at room temperature after standing 30min and measures its absorbance A 1；2mLDPPH solution and the (distillation of 2mL solvents are measured again Water or corresponding buffer solution) mixed absorbance A 3；After finally measurement 2mL test samples solution is mixed with 2mL absolute ethyl alcohols Absorbance A 2.
Calculation formula：Clearance rate (%)=[1- (A1-A2)]/A3 × 100%
Clearance rate shows that more greatly oxidation resistance is stronger, the results are shown in Table 3
Table 3
Clearance rate (%)
Formula group 1 94.9
Formula group 2 82.1
Formula group 3 86.3
Formula group 4 84.5
Formula group 5 82.7
Formula group 6 89.6
Formula group 7 79.9
Formula group 8 80.7
Formula group 9 84.1
Formula group 10 85.5
1.3 safety testing
1.3.1 skin sensibiligen is tested
Wistar rats 10 are chosen, half male and half female, weight 200g takes sample by the dosage of 5000mg/kg weight, applies It is put on back part of animal hair removal section (range 30-40cm2), it is covered with two layers of template, it is fixed for 24 hours with the closing of nonirritant adhesive plaster. Animal subject is removed after for 24 hours, observes animal poisoning sign, observation period 7d.As a result it shows：Formula group is without rat without death.
1.3.2 more Skin Irritation Tests
Choose Wistar rats 10, weight 200g.Sample is taken by the dosage of 5000mg/kg weight, is applied to animal Back hair removal section (range 30-40cm2), fixed 1h is closed with nonirritant adhesive plaster, left side hair removal section is control, 1 time a day, even Continuous 20d.Since the 2nd day, tested material is cleaned with distilled water before smearing tested material every time, skin wound repair knot is observed after 1h Fruit.As a result it shows：Formula group 1-4 does not occur irritation, and phenomena such as skin allergy, red and swollen occurs in formula group 5-10.
Two, craft screening
It is in raw material components：Component A：Honeysuckle flower extract 5g, Rhizoma Gastrodiae extract 1g, Ganodenna Lucidum P.E 1g, B component：Palm fibre Palmitic acid acyl tripeptides -11g, Argireline 0.1g, component C：Polyglutamic acid 1.5g, D component：Essence auxiliary material and essence matrix On the basis of 8g, following several techniques are respectively adopted and are prepared into essence：
2.1 light spot maintenance effect
Melanin genesis in mouse melanin cell is inhibited to test：
By B16 mouse melanin tumor cells with 1 × 105The density of a/ml is connected to the culture dish of a diameter of 9cm, in 37 DEG C ﹑ 5%CO2Under conditions of cultivate 2 days, abandon supernatant, the sample solution 18-22ml after filtration sterilization be added, is then incubated for 3 days, abandons Supernatant, PBS washings, the trypsin solution vitellophag 3min of 0.5ml mass percents 0.2% is added per hole, adds 2ml to tie up per ware Hold liquid terminate digestion piping and druming mixing after, each concentration take out 0.5ml do remaining cell suspension of cell count with 2500r/min from Heart 5min, abandons supernatant, and NaoH solution is added in precipitation, and heating makes melanin dissolve, and selects the enzyme linked immunological of 490nm wavelength Absorbance value is surveyed on detector.
B16 cell inhibiting rate=(1- (drug hole absorbance+drug hole cell density)+(control wells absorbance value+right According to hole cell density)) × 100%.
Melanin genesis in mouse melanin cell is inhibited to test, the results are shown in Table 4
Table 4
Inhibiting rate (%)
Technique groups 1 93.5
Technique groups 2 83.6
Technique groups 3 82.1
Technique groups 4 86.5
Technique groups 5 89.1
Technique groups 6 81.9
Technique groups 7 81.2
2.2 anti-oxidation efficacy
Selection DPPH free radical scavenging abilities are tested to test composition oxidation resistance：
The DPPH solution that 0.1mmol/L is prepared with absolute ethyl alcohol, is kept in dark place, test sample solution is configured to a concentration of The solution of 0.5mg/mL, the test sample solution and DPPH solution for drawing 2mL respectively with pipettor are added in same test tube, fill Divide and shake up, is protected from light at room temperature after standing 30min and measures its absorbance A 1；2mLDPPH solution and the (distillation of 2mL solvents are measured again Water or corresponding buffer solution) mixed absorbance A 3；After finally measurement 2mL test samples solution is mixed with 2mL absolute ethyl alcohols Absorbance A 2.
Calculation formula：Clearance rate (%)=[1- (A1-A2)]/A3 × 100%
Clearance rate shows that more greatly oxidation resistance is stronger, the results are shown in Table 5
Table 5
Clearance rate (%)
Technique groups 1 94.8
Technique groups 2 84.8
Technique groups 3 83.1
Technique groups 4 86.6
Technique groups 5 80.2
Technique groups 6 83.8
Technique groups 7 85.1
2.3 safety testing
2.3.1 skin sensibiligen is tested
Wistar rats 10 are chosen, half male and half female, weight 200g takes sample by the dosage of 5000mg/kg weight, applies It is put on back part of animal hair removal section (range 30-40cm2), it is covered with two layers of template, it is fixed for 24 hours with the closing of nonirritant adhesive plaster. Animal subject is removed after for 24 hours, observes animal poisoning sign, observation period 7d.As a result it shows：Technique groups are without rat without death.
2.3.2 more Skin Irritation Tests
Choose Wistar rats 10, weight 200g.Sample is taken by the dosage of 5000mg/kg weight, is applied to animal Back hair removal section (range 30-40cm2), fixed 1h is closed with nonirritant adhesive plaster, left side hair removal section is control, 1 time a day, even Continuous 20d.Since the 2nd day, tested material is cleaned with distilled water before smearing tested material every time, skin wound repair knot is observed after 1h Fruit.As a result it shows：Technique groups do not occur irritation.
Specific implementation mode
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited in these realities Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit Range.
Embodiment 1
One kind having both light spot maintenance, oxidation resistant skin care compositions, including：
Component A：7g honeysuckles flower extract, 3g Rhizoma Gastrodiae extracts, 2g Ganodenna Lucidum P.Es；
B component：0.52g palmityl tripeptides -1,0.12g Argirelines；
Component C：1.8g polyglutamic acid；
The preparation method of component A：It takes Rhizoma Gastrodiae, ganoderma lucidum bulk pharmaceutical chemicals to clean up respectively, is placed under the conditions of temperature is 77 DEG C and dries After dry, slice obtains gastrodia elata slice, ganoderma lucidum slice；Gastrodia elata slice, ganoderma lucidum slice, honeysuckle flowers are separately added into 10 times of amounts of above-mentioned raw materials medicine weight Water impregnates 25min, is heated to 90 DEG C, decocts 1.0h, liquid is filtered to obtain, by liquid through vacuum-concentrcted to bulk pharmaceutical chemicals weight 3 times, filtering, take filtrate respectively as honeysuckle flower extract, Rhizoma Gastrodiae extract, Ganodenna Lucidum P.E, extract be uniformly mixed；
The skin care compositions be applied to facial mask, eye cream, face cream, essence, mildy wash, toner, lotion, hydrosol, every Production from frost.
Embodiment 2
One kind having both light spot maintenance, oxidation resistant skin care compositions, including：
Component A：10g honeysuckles flower extract, 5g Rhizoma Gastrodiae extracts, 5g Ganodenna Lucidum P.Es；
B component：1.2g palmityl tripeptides -1,0.2g Argirelines；
Component C：3g polyglutamic acids；
The preparation method of component A：It takes Rhizoma Gastrodiae, ganoderma lucidum bulk pharmaceutical chemicals to clean up respectively, is placed under the conditions of temperature is 82 DEG C and dries After dry, slice obtains gastrodia elata slice, ganoderma lucidum slice；Gastrodia elata slice, ganoderma lucidum slice, honeysuckle flowers are separately added into 20 times of amounts of above-mentioned raw materials medicine weight Water impregnates 35min, is heated to 100 DEG C, decocts 2.0h, liquid is filtered to obtain, by liquid through vacuum-concentrcted to bulk pharmaceutical chemicals weight 5 times, filtering, take filtrate respectively as honeysuckle flower extract, Rhizoma Gastrodiae extract, Ganodenna Lucidum P.E, extract be uniformly mixed；
The skin care compositions be applied to facial mask, eye cream, face cream, essence, mildy wash, toner, lotion, hydrosol, every Production from frost.
Embodiment 3
One kind having both light spot maintenance, oxidation resistant skin care compositions, including：
Component A：5g honeysuckles flower extract, 1g Rhizoma Gastrodiae extracts, 1g Ganodenna Lucidum P.Es；
B component：0.05g palmityl tripeptides -1,0.05g Argirelines；
Component C：0.1g polyglutamic acids；
The preparation method of component A：It takes Rhizoma Gastrodiae, ganoderma lucidum bulk pharmaceutical chemicals to clean up respectively, is placed under the conditions of temperature is 80 DEG C and dries After dry, slice obtains gastrodia elata slice, ganoderma lucidum slice；Gastrodia elata slice, ganoderma lucidum slice, honeysuckle flowers are separately added into 16 times of amounts of above-mentioned raw materials medicine weight Water impregnates 30min, is heated to 95 DEG C, decocts 1.5h, liquid is filtered to obtain, by liquid through vacuum-concentrcted to bulk pharmaceutical chemicals weight 4 times, filtering, take filtrate respectively as honeysuckle flower extract, Rhizoma Gastrodiae extract, Ganodenna Lucidum P.E, extract be uniformly mixed；
The skin care compositions be applied to facial mask, eye cream, face cream, essence, mildy wash, toner, lotion, hydrosol, every Production from frost.
Embodiment 4
On the basis of embodiment 1-3 is any, light spot maintenance is had both, oxidation resistant skin care compositions further includes D component 10g, Applied to production any in essence, toner, hydrosol, facial mask, production method is：
D groups are placed in stirring container, 65-70 DEG C is warming up to the rate of 3-4 DEG C/min, stirring, heat preservation 5min with On, then with the rate of 3-5 DEG C/min it is cooled to 35-40 DEG C, component A is added, stirs 10-15min, so that material is uniformly mixed, so Essence, B component, component C are added afterwards and stirs 10-15min, discharges, stands；
The D components include：35g purified waters, 22g glycerine, 0.1g laruyl alcohol sodium sulfovinates.
Embodiment 5
On the basis of embodiment 1-3 is any, light spot maintenance is had both, oxidation resistant skin care compositions further includes D component 15g, Applied to production any in face cream, eye cream, mildy wash, lotion, sun screen, production method includes the following steps：
S1 water phases are pre-dissolved：Glycerine and purified water in D components are placed in the water phase jacketed pan of vacuum emulsifying machine, with 3-4 DEG C/rate of min is warming up to 85-90 DEG C, stirring is opened, after being stirred 5-10min, laruyl alcohol is added in 85 turns/min of rotating speed Sodium sulfovinate is uniformly dispersed；
S2 oil phases are pre-dissolved：Cosmetic base in addition to essence is placed in the oil phase jacketed pan of vacuum emulsifying machine, with 3-4 DEG C/rate of min is warming up to 85-90 DEG C, stirring is opened, 85 turns/min of rotating speed is stirred dissolving；
S3 is emulsified：Water phase, oil phase material are sent into emulsification pot, turn/min, vacuum degree in rotating speed 2500-2800:- 0.03--0.04MPa, the emulsified 5-10min of 70-80 DEG C of condition of temperature are cooled to 35-40 DEG C with the rate of 3-5 DEG C/min, add Enter component A, stir 10-15min, material is made to be uniformly mixed, essence, B component, component C is then added and stirs 10-15min, goes out Material is stood；
The D components include：43g purified waters, 24g glycerine, 0.2g laruyl alcohol sodium sulfovinates；
The cosmetic base is stearine, cetanol, stearyl alcohol, isopropyl palmitate, polydimethylsiloxanes The composition of alkane, tocopherol, essence, methyl hydroxybenzoate, Nipasol.

Claims (8)

1. having both light spot maintenance, oxidation resistant skin care compositions, which is characterized in that in parts by weight include 7-20 parts of component As, 0.1-1.4 parts of B components, 0.1-3 parts of component Cs；The component A is honeysuckle flower extract, Rhizoma Gastrodiae extract, Ganodenna Lucidum P.E；Institute It is palmityl tripeptides -1, Argireline to state B component；The component C is polyglutamic acid.
2. having both light spot maintenance, oxidation resistant skin care compositions as described in claim 1, which is characterized in that the skin care group Object is closed, further includes in parts by weight：8-15 parts of D components, D groups are divided into cosmetic or cosmetic base.
3. having both light spot maintenance, oxidation resistant skin care compositions as claimed in claim 2, which is characterized in that the cosmetics Auxiliary material includes in parts by weight：32.5-69.7 parts of purified waters, 15-28 parts of glycerine, 0.1-0.3 parts of laruyl alcohol sodium sulfovinates.
4. having both light spot maintenance, oxidation resistant skin care compositions as claimed in claim 2, which is characterized in that the cosmetics Matrix is stearine, cetanol, stearyl alcohol, isopropyl palmitate, dimethyl silicone polymer, tocopherol, essence, oxybenzene Any one in methyl esters, Nipasol or two or more compositions.
5. having both light spot maintenance, oxidation resistant skin care compositions as described in claim 1, which is characterized in that the component A with Parts by weight meter includes：5-10 parts of honeysuckle flower extracts, 1-5 parts of Rhizoma Gastrodiae extracts, 1-5 parts of Ganodenna Lucidum P.Es.
6. having both light spot maintenance, oxidation resistant skin care compositions as described in claim 1, which is characterized in that the B component with Parts by weight meter includes：0.05-1.2 parts of palmityl tripeptides -1,0.05-0.2 parts of Argirelines.
7. having both light spot maintenance, oxidation resistant skin care compositions as claimed in claim 4, which is characterized in that the component A, Preparation method：It takes Rhizoma Gastrodiae, ganoderma lucidum bulk pharmaceutical chemicals to clean up respectively, is placed under the conditions of temperature is 77-82 DEG C after drying, slice, Obtain gastrodia elata slice, ganoderma lucidum slice；Gastrodia elata slice, ganoderma lucidum slice, honeysuckle flowers be separately added into above-mentioned raw materials medicine weight 10-20 times measure water, impregnate No less than 25min is heated to 90-100 DEG C, decocts 1.0-2.0h, liquid is filtered to obtain, by liquid through vacuum-concentrcted to raw material 3-5 times of medicine weight, filtering, takes filtrate respectively as honeysuckle flower extract, Rhizoma Gastrodiae extract, Ganodenna Lucidum P.E, by extract It is uniformly mixed to obtain the final product.
8. having both light spot maintenance as described in claim 1, oxidation resistant skin care compositions is applied to facial mask, eye cream, face cream, essence The production of Hua Su, mildy wash, toner, lotion, hydrosol, sun screen.
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CN113101237A (en) * 2021-04-16 2021-07-13 东晟源研究院(广州)有限公司 Polyglutamic acid with anti-wrinkle effect and application of polyglutamic acid in cosmetics

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109316421A (en) * 2018-12-03 2019-02-12 吕伟 Compound ion active membrane
CN113101237A (en) * 2021-04-16 2021-07-13 东晟源研究院(广州)有限公司 Polyglutamic acid with anti-wrinkle effect and application of polyglutamic acid in cosmetics

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