CN108720019A

CN108720019A - A kind of nutrition brewed powder and preparation method thereof that Cancer related fatigue is mitigated or eliminated

Info

Publication number: CN108720019A
Application number: CN201810959510.XA
Authority: CN
Inventors: 谢梦洲; 张超文; 王亚敏; 宋毓涛; 陈光宇; 瞿昊宇
Original assignee: Hunan University of Chinese Medicine
Current assignee: Hunan University of Chinese Medicine
Priority date: 2018-08-22
Filing date: 2018-08-22
Publication date: 2018-11-02

Abstract

The invention discloses a kind of nutrition brewed powders and preparation method thereof that Cancer related fatigue is mitigated or eliminated, and by mass parts, the nutrition brewed powder is made of following raw material：Chinese medicine constituents：1-5 parts of ginseng, 5-10 parts of Poria cocos, 4-8 parts of Semen Lablab Album, 5-10 parts of Chinese yam, 4-9 parts of lotus seeds, 1-5 parts of dried orange peel, 4-9 parts of coix seed, 5-10 parts of the fruit of Chinese wolfberry, 5-10 parts of sealwort；Five cereals constituents：3-8 parts of bitter buckwheat, 3-10 parts of black rice, 3-8 parts of black soya bean, 1-4 parts of polished rice；Additive kind ingredient：1-6 parts of kudzu-vine root powder, 1.5-3 parts of konjaku flour, 0.5-3 parts of whole milk powder, 1-8 parts of xylitol, 1-5 parts of purple sweet potato powder, 1-4 parts of red date powder.Join the in good taste of Siberian cocklebur paddy perfume (or spice) brewed powder, there is the Cancer related fatigue effect that is mitigated or eliminated, preparation method is easy, is suitble to large-scale production.

Description

A kind of nutrition brewed powder and preparation method thereof that Cancer related fatigue is mitigated or eliminated

Technical field

The present invention relates to a kind of nutrition brewed powders and preparation method thereof that Cancer related fatigue is mitigated or eliminated.

Background technology

Herbal cuisine is one of the important component of motherland's medicine, is the rarity of Chinese traditional culture, be China for thousands of years It continues to develop, the knowledge cultural heritage constantly accumulated, and is a knowledge for needing constantly to inherit innovation.With the change in epoch It moves, scientific and technical development, herbal cuisine needs to combine modern section on adhering to Basic Theories of Chinese Medicine and the dialectical principle basis for applying meals Skill is innovated, and is allowed to while ensureing quality, and it is also increasingly convenient to eat, to create one for the development of medicative diet for food therapy health The new road of item.

The trend that rises year by year is presented in cancer morbidity in recent years, along with the transformation of Novel medical pattern, medical and nursing work people Member more focuses on the raising of patients ' life quality on the basis of oncotherapy effect.Influence the factor of quality of life in cancer patients More, wherein Cancer related fatigue (Cancer-related fatigue, CRF) causes the extensive attention of medical worker.Due to CRF , duration rapid with progression of the disease be long and the clinical characters such as prognosis is bad, for patient's daily life and prognosis rehabilitation shadow Sound is larger.The definition of Cancer related fatigue (CRF) not yet carries out Uniform provisions both at home and abroad, according to pertinent literature, by American National The CRF concepts that cancer comprehensive network (National comprehensive cancer network, NCCN) defines, are defined For：" a kind of Subjective fatigue feeling of duration, it is related to cancer or treatment of cancer and unrelated with recent activity, and interfere Normal life ".CRF is patient in experience tumour and the subjective feeling appeared in treatment neoplastic process, clinical symptoms performance Tired for energy, depressed, cognitive ability declines, hebetude, can not be engaged in original adequate work, and tired journey Degree is uncorrelated to activity condition, closely related with tumour and treatment means, and patient after abundance sleep and rest it is tired still without Method is alleviated.With the continuous progress studied Quality of Life of Patients with Tumor, CRF increasingly attracts attention.But the country is to CRF at present Research it is less, prevention and treatment means also relatively lack, the related datas such as epidemiology and influence factor are less, many doctors couple CRF lacks understanding in master, objective aspects, does not understand patient in depth because of the tired Affliction grade brought, while tumour is suffered from Person itself is also insufficient to the degree of awareness of CRF, can receive disease, thirst for existence and partner treatment, but be difficult to fling to tired Sense has aggravated psychological burden, and patient often shows as dejected, helplessness and self-accusation, need strongly a kind of health it is comfortable, without tired Weary animation.

Cancer related fatigue is extremely common and little with tumor type relationship in tumor patient, and previous research is reported in always At least 50% patient will appear CRF in body tumor patient, and most patient occurs in tumor therapeutic procedure It crosses tired, or even there is 40% patient just to have tired symptom before diagnosing tumor.In Cancer related fatigue and cancer patient's body The immune system defense mechanism of activation is related.Definite final conclusion there is no for the Mechanism Study of Cancer related fatigue at present, treat Effective means is also lacked, the organic conception of Chinese medicine and diagnosis and treatment principle have highlighted its advantage in the case.Li Zhongzi recognizes For:" blood rooting in the kidney, QI being derived from the spleen, and people's money with for beginning person." the kidney being the origin of congenital constitution, main bone gives birth to marrow, Kidney qi foot then contain by essence and blood, and then blood and essence asthenia, body fluid metabolism are not normal for kidney qi deficiency；The spleen being the foundation of acquired constitution, the source of vital function, main transporting are main System blood, spleen deficiency are not transported then, no resource of generating and transforming QI and blood.Deficiency of spleen and stomach causes kidney not store essential substances, and essence does not give birth to marrow, in addition spleen inability of food and drink to be digested and transported It is precise and tiny, lead to that nutrient absorption is bad, metabolism disorder, deficiency in both Qi and blood, the five internal organs, which lose, supports, all kinds of diseases and ailments and give birth to.Therefore Many researchers use Strengthening spleen, tonifying kidney method makes the strong fortune of temper, and kidney yang is sufficient, raises healthy tendency, is supported to control to the ill to the Cancer related fatigue of tumor patient It treats, all achieves certain effect.

Drug food of TCM, which is in drug food of TCM, using food or other natural nutrient substances, to be come under instruction of Chinese Medicine theory Health body-building, prevention and treatment disease promote physical recovery, and the subject to slow down aging.Drug food of TCM is derived from " medicine Eat homologous ", true essence is " three drug dispensings seven are divided foster ", " Diet cures more than the doctors ".In the cognition of Chinese medicine, CRF can belong to " empty Labor " scope, internal negative and positive of qi and blood imbalance, internal organs vigour virtual loss caused by tumor therapeutic procedure form, with the passing of time sthenia symptoms caused by asthenia. Diagnosis and treatment is the basis of Chinese traditional treatment, studies have shown that with asthenic symptoms being more in CRF card types, real example is less.Efficacy of drugs is mostly to help Based on just consolidating, meet the principle of reatment of asthenic symptoms.In tcm causa morbi analysis, dietary factor is all always important as one Pathogenic factor, and drug food of TCM has unique effect in terms of preventing and treating chronic chronic diseases marked by deficiency of vital energy disease, therefore in middle medical knowledge Under the guidance of opinion, using modern science and technology, easy from manufacture craft, innovation angle is produced a various tumours of improvement and is suffered from The product of person's Cancer related fatigue has wide social effect.

Nutrient grain brewed powder class product is increasing on the market, it is praised highly the effect of type various kinds, multiple tastes.Than The more famous five cereals for having the brands such as Yan Zhifang, health mill, five cereals mill to produce reconstitutes i.e. baking soda and is just deeply loved by the public. As a kind of convenient and efficient and nutrition meal replacement powder, for middle blueness is for working clan, such meal replacement powder not only quickly but also Convenient nutrition need to only brew, and the time is short, simple for production；For the elderly, such meal replacement powder functionality is various, Particle is tiny, and mouthfeel is more smooth, is swallowed without chewing, convenient and instant, therefore well received in old group.Seen in currently on the market Cereal brewed powder be mostly direct baking curing beat powder after mix, directly it is canned, then each one according to oneself need tune amount food With, though while raw materials have integration of drinking and medicinal herbs drug, type is more single, therefore necessary requirement is often not achieved in effect, and Ginseng Siberian cocklebur paddy perfume (or spice) brewed powder is basic as cereal using lotus seeds, coix seed, bitter buckwheat, black soya bean, black rice, polished rice, then adjusts and add kudzu-vine root powder, konjaku The Chinese medicine preparation of powder, xylitol, milk powder, purple sweet potato powder, red date powder cooperation integration of drinking and medicinal herbs Chinese medicine preparation extract by spray drying A nutrition brewed powder obtained by powder.The product major ingredient possesses seven kinds of colors, i.e., after the ginger color of Chinese medicine preparation powder, lotus seeds curing Light beige, bitter buckwheat light green color after curing, black soya bean deep ecru after curing, black rice atropurpureus after curing, polished rice it is ripe The darkviolet of pure white, purple sweet potato powder after change.Total finished product reconstitutes out in aubergine last, and perhaps this can visually give A kind of enjoyment of people increases appetite.

Invention content

Present invention solves the technical problem that being herbal cuisine or the preparation that Cancer related fatigue is mitigated or eliminated in the prior art Or method is complicated or mouthfeel is bad or drug effect is bad, contains excessive traditional Chinese medicine ingredients in formula, it is unsuitable for a long time, Large dosage is taken.

The technical scheme is that providing a kind of nutrition brewed powder (present invention general that Cancer related fatigue is mitigated or eliminated The product is known as：Join Siberian cocklebur paddy perfume (or spice) brewed powder), by mass parts, the nutrition brewed powder is made of following raw material：

Chinese medicine constituents：1-5 parts of ginseng, 5-10 parts of Poria cocos, 4-8 parts of Semen Lablab Album, 5-10 parts of Chinese yam, 4-9 parts of lotus seeds, dried orange peel 1-5 parts, 4-9 parts of coix seed, 5-10 parts of the fruit of Chinese wolfberry, 5-10 parts of sealwort；

Five cereals constituents：3-8 parts of bitter buckwheat, 3-10 parts of black rice, 3-8 parts of black soya bean, 1-4 parts of polished rice；

Additive kind ingredient：1-6 parts of kudzu-vine root powder, 1.5-3 parts of konjaku flour, 0.5-3 parts of whole milk powder, 1-8 parts of xylitol are purple 1-5 parts of potato powder, 1-4 parts of red date powder.

Preferably, the nutrition brewed powder is made of following raw material：

Chinese medicine constituents：4.7 parts of ginseng, 8.7 parts of Poria cocos, 6.7 parts of Semen Lablab Album, 8.7 parts of Chinese yam, 6.7 parts of lotus seeds, dried orange peel 3.3 parts, 6.7 parts of coix seed, 8.7 parts of the fruit of Chinese wolfberry, 8.7 parts of sealwort；

Five cereals constituents：5 parts of bitter buckwheat, 7.5 parts of black rice, 5 parts of black soya bean, 2.5 parts of polished rice；

Additive kind ingredient：4.7 parts of kudzu-vine root powder, 0.3 part of konjaku flour, 1.7 parts of whole milk powder, 5.3 parts of xylitol, purple sweet potato powder 2 parts, 1.3 parts of red date powder.

The preparation method of above-mentioned nutrition brewed powder, includes the following steps：

It decocts, filters after Chinese medicine constituents are impregnated, after filtrate is condensed into medicinal extract, extract powder is made in spray drying；By five Cereal component cures, and crushes, five flours are made；Extract powder, five flours and additive kind ingredient are mixed, nutrition is obtained and reconstitutes Powder.

The preparation method of above-mentioned nutrition brewed powder, includes the following steps：By ginseng, Semen Lablab Album, dried orange peel, Chinese yam, sealwort powder 10 mesh of broken mistake sieves, and Poria cocos, matrimony vine do not crush, and above seven taste decocts after immersion, filtering, after filtrate is condensed into medicinal extract, spray drying Extract powder is made；It by lotus seeds, coix seed, the curing of five cereals constituents, crushes, five flours is made；By extract powder, five flours and addition Agent constituents mix, and obtain nutrition brewed powder.

Preferably, it after adding maltodextrin in medicinal extract, then is spray-dried.

Preferably, it is 2 times to decoct number, and first time amount of water is 16 times of quality of medicinal material, and water decocts time 2 h；The 2 amount of water are 10 times of quality of medicinal material, water decocting time 1 hour.

The herbal cuisine Chinese medicine preparation powder carries out the side of tearing open based on shenling baizhu powder, then is subject in integration of drinking and medicinal herbs class help simultaneously Medicine sealwort, matrimony vine tonifying kidney and nourishing yin invigorating the spleen, solid protection human righteousness.Traditional Chinese medicine thinks that disease is due to perverse trend Sheng, healthy tendency Weak, tumour is also such.The spleen and stomach provide the material basis of the acquired, is human body source of generating QI and blood, therefore function of spleen and stomach regulating is solid protection human righteousness Important channel.

The herbal cuisine is portable nutrition brewed powder, as a kind of conveniently nutritious food product, current allegro Liked deeply by each age group crowd in city life.For urbanite since rhythm of life is fast, pressure is big, thus make portable fast food at For a kind of current popular form.But functional cereal brewed powder product and few, some and warp currently on the market Product that is puffing or digesting, this kind of product raw material may result in some raw material functional components after puffing or enzymolysis and therefore become Property, it is unhelpful to health, therefore this medicated diet product is a from the aspect of simple, mouthfeel, effect three from material treatment process Integrate the herbal cuisine class brewed powder of mouthfeel effect.

The preparation method of the herbal cuisine is：By Chinese medicine (ginseng, Semen Lablab Album, dried orange peel, Chinese yam, sealwort crushed 10 mesh, matrimony vine, Poria cocos does not crush), it soaks, then by decocting, filtering, after filtrate concentration, addition auxiliary material is spray-dried, finally One of base stock to Chinese medicine preparation powder as brewed powder, remaining primary raw material is lotus seeds, coix seed, bitter buckwheat, black rice, black Beans, polished rice.Additive kind:Kudzu-vine root powder, konjaku flour, the auxiliary material are flavoring agent and thickener.

Join Siberian cocklebur paddy perfume (or spice) brewed powder formulating

Shenling baizhu powder comes from《The formulary of peaceful benevolent dispensary》, we be on the basis of Sijunzi Tang plus Chinese yam, lotus seeds, Semen Lablab Album, coix seed, fructus amomi, campanulaceae composition, embody " reinforcing earth to generate metal " therapy, for the common prescription for the treatment of insufficiency of the spleen with overabundance of dampness card. Ginseng invigorating middle warmer and replenishing QI invigorating the spleen in side, sealwort is sweet, flat, returns spleen, lung, kidney channel, has the function of boosting qi and nourishing yin invigorating the spleen, moistening lung kidney-nourishing, Matrimony vine has nourishing the liver and kidney, and the effect of yin-nourishing improving eyesight, three is combined into monarch drug in a prescription.Poria cocos clearing damp and promoting diuresis, invigorating the spleen calming heart；Semen Lablab Album is mended Spleen QI invigorating, dampness elimination is in；Chinese yam is invigorated the spleen and benefited qi puckery essence, and three medicines match with monarch drug in a prescription, and the power of strengthening the spleen and replenishing qi clearing damp is stronger, is minister Medicine.Coix seed invigorating the spleen to arrest diarrhea, inducing diuresis with bland drug, and excreting dampness make damp evil from lower and go under；Lotus seeds supplementing the kidney to control the nocturnal, is supported at invigorating the spleen to arrest diarrhea Heart and tranquilizing mind is adjutant altogether.Dried orange peel qi-regulating eliminating dampness, be amusing with stomach be make medicine.All medicines joint, play altogether invigorating the spleen calming heart, liver-nourishing kidney-boosting it Effect.Modern pharmacology effect also indicates that:It is bad that shenling baizhu powder can improve proleulzin (IL-2), interferon-γ (IFN-γ), tumour Necrosis factor-α (TNF-α) cytokine levels, antitumor action and adjusting body's immunity.Related ginseng is to entity tumor It is its important component to have certain antitumor curative effect, ginsenoside.Rhizoma Atractylodis Macrocephalae main component be Baizhu volatile oil, polysaccharide and Lactone constituents etc., wherein Baizhu volatile oil, which have, inhibits cancer cell multiplication effect, and soluble polysaccharide, which has, improves immunity, Lactone constituents have the function of invigorating the spleen fortune spleen, adjust gastrointestinal function and promote absorption of nutrient ingredients.Poria cocos of the Poria cocos contained by it Polysaccharide and ethyl acetate have antitumor action, and there are regular hours and dose-effect relationship.Multiple components in coix seed, such as heart of a lotus seed Seed of jog's tears oil, coixenolide, triglycerides etc. play the effect of anticancer through a variety of ways.

Shenling baizhu powder, which has antitumor action mainly, to be had improvement appetite, increases weight, mitigate symptom, improve existence The effect of the treatment carcinemias such as quality, anemia, promotion seralbumin synthesis, can be through each of oncotherapy Stage.Clinical observation confirms that shenling baizhu powder can improve the state of mind of late tumor patient, improves a poor appetite, and alleviates symptom, changes Kind TCM syndrome, promotes leucocyte, hemoglobin level, improves patient's muscle power situation.Combination therapy evening is supported with conventional nutrient Phase tumor patient, can yet be regarded as improves the effective ways of Quality of Life of Tumor Patients.《Doctor's mirror at all times》Described in shenling baizhu powder More dried orange peels simply again, suitable for the deficiency of spleen-QI and stomach-QI person that has retention of damp leading to blockage of QI concurrently.《The formulary of peaceful benevolent dispensary》Volume three：" weakness of the spleen and the stomach, drink Food not into, more tired few power, in full ruffian choke, the alarmed and panicky asthma of the heart, vomiting and diarrhea and typhoid fever cough.Shenling baizhu powder cures mainly indigestion, The spleen deficiency such as chest gastral cavity ruffian, increased borborygmus and diarrhea, weakness of limbs, body are become thin, and sallow complexion, pale tongue with white fur is greasy, and feeble pulse is slow press from both sides wet card. It is pressed from both sides suitable for deficiency of spleen-QI and stomach-QI wet, it can also be used to which lung damages all diseases of consumptive disease, is the common prescription of " reinforcing earth to generate metal " therapy.This side application is wide General, curative for effect, clinic passes through diagnosis and treatment based on an overall analysis of the illness and the patient's condition, and no matter diseases caused by external factors or all cards of internal injury can be added and subtracted as long as meeting traditional Chinese medicine basic theory With this side, good effect is all taken.But mouthfeel is often barely satisfactory when these traditional Chinese medicines treat disease, to a certain extent It is unfavorable for the improvement of patient symptom.《The Ling Shu Miraculous Pivot or Divine Axis five tastes》It says:" trough all enters stomach, and the vital organs of the human body all report gas in stomach.《Ling Shu Miraculous Pivot or Divine Axis The hygienic meeting of battalion》It says:" people is bullied in paddy, and paddy enters stomach, and to pass and lung, the vital organs of the human body, all to be bullied, lucidity pertaining to ying, turbid person is It defends.《The section of Plain Questions six is hidden as opinion》:" five tastes entrance is hidden in stomach, and taste is hidden, with support five gas, gas and and give birth to, body fluid coordinates, God is spontaneous ".It is the cereal nutrient progress vital movement for receiving food institute metaplasia from stomach to highlight the vital organs of the human body, illustrates diet The five tastes play an important role in vital movement, and rational diet contributes to internal organs battalion to defend the physiological function brought into normal play.Therefore will Chinese medicine is combined with dietotherapy, i.e., " residence doctor is in food " just seems particularly necessary.In tumor patient therapeutic process, largely Cancer related fatigue symptom will occur.For theory of traditional Chinese medical science, it is the performance of consumptive disease, and combines that " The spleen and stomach provide the material basis of the acquired, qi and blood The source of vital function ", " the kidney generating marrow and dominating bone ", " chronic disease involving kidney " scheduling theory, it is believed that spleen kidney deficiency weak (damage) is that it is fallen ill originally.

Tumour is the problem in current China or even the world, and incidence is high, and the death rate is also high.No matter in city or rural area, Tumour is all the underlying cause of death of Chinese residents, and Cancer in China incidence accounts for about the whole world 22%, and the death rate accounts for about the 27% of the whole world.And Cancer related fatigue is a kind of tired sense related with cancer or treatment of cancer or sense tired out, is prevalent in and receives Radiotherapy chemotherapy In patient.National Cancer comprehensive network (National comprehensive cancer network, NCCN) defines CRF concepts, are defined as：" a kind of Subjective fatigue of duration feels, it is related to cancer or treatment of cancer and with recent work It is dynamic unrelated, and interfere normal life ".Cancer related fatigue is extremely common and little with tumor type relationship in tumor patient, The patient that previous research is reported in overall nodule patient at least 50% will appear CRF, and several in tumor therapeutic procedure Whole patients occurred tired, or even had 40% patient just to have tired symptom before diagnosing tumor.In Chinese medicine Cognition on, CRF can belong to " consumptive disease " scope, the internal negative and positive of qi and blood imbalance caused by tumor therapeutic procedure, internal organs member Gas virtual loss forms, with the passing of time sthenia symptoms caused by asthenia.Diagnosis and treatment is the basis of Chinese traditional treatment, studies have shown that being with asthenic symptoms in CRF card types More, real example is less.Therefore efficacy of drugs meets the principle of reatment of asthenic symptoms mostly based on strengthening the body resistance to consolidate the constitution.Li Zhongzi thinks:" blood it Kidney is lain in source, QI being derived from the spleen, and people's money with for beginning person." therefore many researchers by using strengthening spleen, tonifying kidney Method is transported to make temper be good for, and kidney yang is sufficient, raises healthy tendency, supportive treatment to the ill is carried out to the Cancer related fatigue of tumor patient.Join Siberian cocklebur It is to subtract Rhizoma Atractylodis Macrocephalae, fructus amomi, campanulaceae, Radix Glycyrrhizae on the basis of shenling baizhu powder that Gu Xiang, which reconstitutes powder extracts Chinese medicine preparation, adds sealwort, Chinese holly Qi forms.Ginseng tonifying middle-Jiao and Qi in side, shengjin nourishing；Poria cocos and coix seed, hyacinth bean invigorating the spleen excreting dampness, Chinese yam, Semen Nelumbinis invigorating the spleen peace God, arresting seminal emission；Dried orange peel qi-regulating is drying damp and strengthening spleen；Sealwort is sweet, flat, returns spleen, lung, kidney channel, has boosting qi and nourishing yin invigorating the spleen, moistening lung kidney-nourishing Effect, matrimony vine has a nourishing the liver and kidney, the effect of yin-nourishing improving eyesight, two medicines joints the benefits of playing invigorating the spleen liver-nourishing kidney-boosting altogether.All medicines in side It is compatible to have the function of righting, benefiting qi and nourishing blood, immunity of organisms can be improved.It is ginseng in side, sealwort, dried orange peel, Poria cocos, white flat Beans, lotus seeds, Chinese yam, coix seed, matrimony vine anticancer active constituent polysaccharide essentially from water soluble part, while in view of cost is asked Topic, therefore extraction process by water is used, after concentration, adds auxiliary material tune density to carry out low temperature spray drying and ginseng Siberian cocklebur paddy perfume (or spice) brewed powder extraction is made Object low temperature spray drying particle reconstitutes powder raw material as integration of drinking and medicinal herbs herbal cuisine ginseng Siberian cocklebur paddy perfume (or spice).Polysaccharide and content of ginsenoside in the party It is relatively abundant, and the two all has active anticancer.Polysaccharide and ginsenoside R1, Rg1, Rb1 are all dissolved in water, therefore are carried with water as solvent It takes, after filtering, concentrating, then the basic material by its low temperature spray drying at dry extract for the brewed powder.But in mouth In sense, it is spray-dried after extract powder taste it is bitter and have certain tart flavour, therefore need to be by means of some with strengthening spleen, tonifying kidney work( The cereal ingredient of effect adjusts mouthfeel, produces and a not only has effect, but also a nutrition brewed powder of taste good.

Bitter buckwheat：Bitter buckwheat is medicine-food two-purpose crop very few in nature, the biological flavone lacked rich in common cereal crops, The health care compositios such as inositol, Quercetin are known as pure natural green health care food, wherein the rutin being rich in is to diabetes, high blood The patients such as pressure, hyperlipidemia have remarkable effect, are the new grain sources of 21 century health.According to《Compendium of Materia Medica》It records:Bitter buckwheat bitter, it is mild-natured Cold, the real stomach of energy, benefiting energy continues spiritual, sharp knowledge, and refining the five internal organs slag is dirty.Trace elements of selenium is rich in bitter buckwheat, selenium has many institute's weeks The anti-cancer efficacy known, bitter buckwheat are known as " kings of five cereals " again simultaneously.

Black soya bean：Black-food has good health-care efficacy rich in a variety of active ingredients such as polyphenol, flavones, anthocyanidin.It is black Beans are full of nutrition, per 100g foods portion 36g containing protein, fat 15.9g, dietary fiber 10.2g, carbohydrate 33.6g, calcium 224mg, magnesium 243mg, potassium 1377mg, phosphorus 500mg, containing vitamin and trace elements iron needed by human, manganese, zinc, copper, molybdenum, Selenium etc..Black soya bean fat contains more unsaturated fatty acid, phytosterol, and phytosterol can be absorbed by the body, and it is solid to can inhibit courage The absorption of alcohol is the improver of artery sclerosis.Research confirm, black soya bean itself resist peroxidating protection system and black soya bean it is anti-ageing Always, in terms of degeneration-resistant physiology significantly function be black soya bean rich in isoflavones, vitamin E, haematochrome, polysaccharide, glutathione, rutin, The various bioactivators such as Dioscin and superoxide dismutase.Isoflavones in black soya bean is a kind of plant estrogen, Breast cancer, prostate cancer and colon cancer can effectively be inhibited, it is also helpful to prevention person in middle and old age's osteoporosis.Black soya bean kind Pi Yinfu Black is presented containing pigment and with a variety of physiological activity such as anti-oxidant, anti-inflammatory, immunological regulations.Black soya bean polysaccharide is to phagocyte Phagocytic function has immunosuppressive action.《Compendium of Materia Medica》Middle theory：" it is more that black soya bean enters kidney function, therefore can harness the river, lower gas of subsiding a swelling, and controls wind-heat And promoting blood circulation and detoxication." black soya bean plays the role of kidney tonifying, beautifying face and moistering lotion, clear three high (hypertension, hyperglycemia, hyperlipidemias).

Black rice：Black rice is in black or dark brown, and full of nutrition, food, medical value are high, is known as " black pearl " and " world's rice In king " good reputation.Chinese medicine thinks that black rice has enriching yin and nourishing kidney, improving eyesight promoting blood circulation, invigorating the spleen warm stomach and other effects.Black rice is also known as black rice, It is the benevolence of gramineae plant black rice paddy, it is warm-natured, it is sweet in flavor.Black rice not only contains abundant protein, 17 kinds of amino acid, fat, dimensions 14 kinds of elements such as raw plain, minerals and Fe, Zn, Cu, also contain dietary fiber, unsaturated fatty acid, anthocyanidin, flavones, biology The physiological activators such as alkali have and remove free radical, the functions such as anti-aging, and have anti allergic reaction and immunoregulation effect Etc. physiological functions, be make nutritional health food desirable feedstock.Black rice not only have abundant nutritive value, and with compared with High medical value.Early in Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》In the effect of just recording " nourishing YIN for benefiting the kidney, improving eyesight promoting blood circulation ", it is existing to face Bed verification, it has preferable therapeutic effect to illnesss such as anaemia, hypertension, neurasthenia, chronic nephritis, indigestions.

Polished rice：Polished rice is the seed of gramineous herbaceous plant rice (japonica rice).Be the shorter circle of grain in rice, viscosity it is relatively strong, The small kind of swollen property.It is sweet in flavor, it is mild-natured.Energy Energy benefit taste, relieving thirst and restlessness.Polished rice is one kind of rice, congee have " first mending in this world " it Laudatory title, it can reduce cholesterol, reduce the probability of heart attack and apoplexy.

Kudzu-vine root powder：Xin Liang, it is sweet in flavor, pungent, tool inducing diaphoresis brings down a fever, promotes the production of body fluid to quench thirst, Shengyang Zhixie, expelling pathogenic factors from muscles and skin promoting eruption and other effects, Chinese medicine It is usually used in fever caused by exogenous pathogens headache, neck pain is quenched one's thirst and measles without adequate eruption, the cards such as hot dysentery, diarrhea.Modern pharmacological studies have shown that:Pueraria lobota Root has expansion coronary artery blood vessel and improves myocardial ischemia-anoxemia state, and anti-arrhythmia, decompression and antianginal, pueraria lobata also have Improve circulatory function, inhibit platelet aggregation and anticancer effect.Pueraria lobata sobering up and liver protecting functions beverage can effectively play anti- The effect of liquor-saturated relieving alcoholism and protecting livers protect liver.Wang Xin is auspicious et al. to use the pueraria lobata without any extraction, observes it to removal ovary sclerotin The effect of bone density and the bone tissue construction of loose model mice show that pueraria lobata is different from estradiol and significantly stimulates uterus, and has With the comparable function of resisting osteoporosis of estradiol, having can be safe and effective as prevention and treatment women post menopausal osteoporosis disease Food or drug.Research confirms that it is close that pueraria lobata can not only prevent ovary excision oestrogen deficiencies osteoporosis model Mouse Bone The decline of degree and bone amount, and bone microstructure can be improved, it can be used for the prevention and treatment of women post menopausal osteoporosis disease. Similarly, researcher has also investigated whether pueraria lobata have similar curative effect, result to the treatment of osteoporosis It is to determine.

Konjaku flour：Containing abundant dietary fiber, long-term consumption konjak products are conducive to lose weight, and can prevent treating constipation, high blood Sugar and intestinal cancer etc..In addition, konjaku has good thickening property, gelation, water-retaining property etc., it has also become good dietary fiber sources, It is widely used in the fields such as food industry, medicine, daily-use chemical industry.He Ya is beautiful et al. to pass through Amorphophallus Konjac for Ehrlich Ascites Cancer (EAC) The research of inhibiting effect is respectively by konjaku powder (RKM), konjaku ashes, fresh konjak stem block decoction, cyclophosphamide, physiological saline Ehrlich ascites tumor (EAC) intragastric administration on mice is administered, experimental result show that konjaku may be by enhancing body's immunity to press down The growth of transplanted tumor processed, plays antitumor action.Report white konjaku powder (mono- 80cy0 containing KGM70) to 5180 meat of mouse Tumor, EAC Emhorns tumor, HepS liver cancer and U14 cervical carcinomas tumour inhibiting rate be respectively 59.74%, 54.02%, 52.79% and 40.86%, the phagocytic function of tumor-bearing mice mononuclear macrophage can be dramatically increased, T lymphocyte transformation abilities also significantly improve, KGM antitumor activities and the correlation between immunoregulation capability are prompted.Jia Ying etc. using mtt assay to konjacmannan peptide into Row antitumor activity detects, the result shows that, konjacmannan peptide is to colon cancer cell HCT-116 and hepatocellular carcinoma H22 Proliferation all has certain inhibiting effect, and preferable dose dependent is presented in 1~100 μm of ol/L concentration range, is possessed Taro mannatide is better than colon cancer cell HCT-116 to the selectivity of hepatocellular carcinoma H22, and the inhibiting effect being proliferated to it is more It is notable.

The extract dry extract that is equipped with is by ginseng (artificial growth), Poria cocos, Chinese yam, white flat in ginseng Siberian cocklebur paddy perfume (or spice) brewed powder Beans, dried orange peel, sealwort, matrimony vine etc. obtain by extracting, concentrating, be spray-dried, and we are added and subtracted on the basis of shenling baizhu powder ?.In general, the effect of ginseng Siberian cocklebur paddy perfume (or spice) brewed powder has strengthen immunity, improves intestinal flora.

Ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts and measures ginsenoside Rg using HPLC methods₁、Re、Rb₁Content, phenolsulfuric acid ratio Color method measures the content of polysaccharide, and single factor experiment method investigates alcohol extracting, extraction process by water, and response surface design experiment method optimization ginseng Siberian cocklebur paddy is fragrant Powder extracts extraction process by water parameter is reconstituted, foundation is provided for the industrialized production of the medicated diet product.

The invention has the advantages that it is using shenling baizhu powder as base to join Siberian cocklebur paddy perfume (or spice) in the present invention to reconstitute powder extracts formula Plinth carries out the side of tearing open, then is subject to integration of drinking and medicinal herbs class traditional tonic medicine sealwort, matrimony vine tonifying kidney and nourishing yin invigorating the spleen, solid protection human righteousness simultaneously.In Medicine thinks that disease is since perverse trend Sheng, healthy tendency are weak, and tumour is also such.The spleen and stomach provide the material basis of the acquired, is energy and blood of human body The source of vital function, therefore the product formula and raw material are started in line with from taste, and modern food technique is combined, make drug food of TCM theory It preferably digests, and applies in convenient food, preferably embody medicine and food power, food is borrowed to help medicine prestige.Ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes Powder it is in good taste, have and be mitigated or eliminated Cancer related fatigue effect, preparation method is easy, is suitble to mass produce.

Specific implementation mode

1 ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts Study on extraction

1.1 instruments and material

3000 highly effective liquid phase chromatographic systems of Ultimate (silent winged scientific and technological (China) Co., Ltd of generation that of match；Including G1316A Quaternary gradient pump, G1313A standard type autosamplers, G1316A insulating boxs, G1314A UV detector, Agilent Chemstation chromatographic work stations)；UV9100B ultraviolet-uisible spectrophotometers (the limited public affairs of Beijing LabTech instrument share Department)；YP-B5001 types electronic balance (the upper sources sea light Zheng Yi Instrument Ltd.)；RHP-400 type high-speed multifunctional pulverizers (Zhejiang Yongkang City Rong Hao Industrial Co., Ltd)；The water tank (Beijing Zhong Xing great achievements Instrument Ltd.) of SHH.W21 electronics three； III multiplex vavuum pump of circulating water type of SHB- (Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd.)；The old pharmacist's room temperature boiling machine (Changsha YJD20 City's Zhuo is at Medical Devices Co., Ltd.)；RE-200013 Rotary Evaporators (Yuhua Instrument Co., Ltd., Gongyi City).

1.2 medicinal materials and reagent

The medicinal materials such as ginseng, Poria cocos, Semen Lablab Album, dried orange peel, lotus seeds, Chinese yam, coix seed, matrimony vine are purchased from the big pharmacy of common people, yellow Essence is purchased from Xinhua County in Hunan Province Lv Yuan agriculture and forestry science and technologies Co., Ltd, meets through pharmaceutical college of Hunan University of Traditional Chinese Medicine Sunday treasured professor identification 2015 editions《Chinese Pharmacopoeia》Pertinent regulations under one item；Ginsenoside Rg1's reference substance (lot number：In 110703-200726) being purchased from State's food and medicine examines and determine research institute, for assay；Ginsenoside Rb1's reference substance (lot number：In 110704-200921) being purchased from State's food and medicine examines and determine research institute, for assay；Ginsenoside Re's reference substance (lot number：In 110702-200925) being purchased from State's food and medicine examines and determine research institute, for assay；DEXTROSE ANHYDROUS reference substance (lot number：110833- 201506) it is purchased from State's food and medicine examines and determine research institute, for assay；Methanol, acetonitrile are chromatographically pure, and water is double distilled water, other HPLC examinations Agent is all chromatographically pure；The colorimetrics such as phenol, the concentrated sulfuric acid, ether, absolute ethyl alcohol are all domestic analytical reagents with reagent.

1.3 methods and result

1.3.1HPLC method measures the content chromatography that ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes ginsenoside Rg1 and Rb1 in powder extracts Aqueous extracts 3000 chromatographic columns of condition Ultimate；Stationary phase is C₁₈Alkyl linked silica gel；Mobile phase is water-acetonitrile；Run time 30min；Detection wavelength 203nm；30 DEG C of column temperature；The equal > 4000 of theoretical cam curve is calculated separately by ginsenosides Rg1, Re and Rb1, Separating degree > 1.5.

1.3.1.1 the preparation of ginsenosides Rg1, Re and Rb1 reference substance solution

(1) ginsenoside Rg1's reference substance about 10mg is taken, it is accurately weighed (0.0103g), it is placed in 10mL volumetric flasks, is added Ginsenoside Rg1's reference substance solution of 1.03mg/mL is made to scale in methanol constant volume.

(2) ginsenoside Rb1's reference substance about 10mg is taken, it is accurately weighed (0.0101g), it is placed in 10mL volumetric flasks, is added Ginsenoside Rb1's reference substance solution of 1.01mg/mL is made to scale in methanol constant volume.

(3) ginsenoside Re's reference substance about 10mg is taken, it is accurately weighed (0.0104g), it is placed in 10mL volumetric flasks, first is added Alcohol is settled to scale, and ginsenoside Re's reference substance solution of 1.04mg/mL is made.

1.3.1.2 the preparation precision of test solution pipettes each ginseng Siberian cocklebur paddy perfume (or spice) and reconstitutes powder extracts water to put forward sample liquid appropriate, It crosses in 0.22 μm of miillpore filter to sample introduction bottle, obtains ginseng Siberian cocklebur paddy perfume (or spice) and reconstitute powder extracts Aqueous extracts test solution.

1.3.1.3 the preparation of negative controls solution is weighed other medicinal materials for removing ginseng in prescription ratio, is carried according to water The preparation process of liquid and the processing method of test solution prepare negative controls solution.

1.3.2 the preparation of ginsenosides Rg1, Re and Rb1 standard curve and the range of linearity

(1) ginsenoside Rg1's reference substance solution of 1.03mg/mL is taken to be diluted to 100 μ g/mL, 90 μ g/mL, 80 μ successively The concentration of g/mL, 70 μ g/mL, 60 μ g/mL, 50 μ g/mL, 40 μ g/mL, 30 μ g/mL, 20 μ g/mL, 10 μ g/mL, by 2.2.1 Lower 10 μ L of chromatographic separation condition sample introduction measure integrating peak areas value, using integrating peak areas value as ordinate Y, ginsenoside Rg1 Concentration X is that abscissa draws standard curve, the results showed that, equation of linear regression is：Y=0.8028 X+0.1463, R²= 0.9994, i.e. ginsenoside Rg1's reference substance solution concentration linear relationship within the scope of 10 μ of μ g/mL~100 g/mL is good.

(2) ginsenoside Rb1's solution of 1.01mg/mL is taken to be diluted to 100 μ g/mL, 90 μ g/mL, 80 μ g/mL, 70 successively The concentration of μ g/mL, 60 μ g/mL, 50 μ g/mL, 40 μ g/mL, 30 μ g/mL, 20 μ g/mL, 10 μ g/mL, by 2.2.1 lower chromatographies 10 μ L of separation condition sample introduction measure integrating peak areas value, are by ordinate Y, ginsenoside Rb1's concentration X of integrating peak areas value Abscissa draws standard curve, the results showed that, equation of linear regression is：Y=0.7954X+0.2763, R²=0.9997, i.e. people It is good to join saponin(e Rb1 reference substance solutions concentration linear relationship within the scope of 10 μ of μ g/mL~100 g/mL.

(3) ginsenoside Re's solution of 1.04mg/mL is taken to be diluted to 100 μ g/mL, 90 μ g/mL, 80 μ g/mL, 70 μ successively The concentration of g/mL, 60 μ g/mL, 50 μ g/mL, 40 μ g/mL, 30 μ g/mL, 20 μ g/mL, 10 μ g/mL, by 2.2.1 lower chromatographies point From 10 μ L of condition sample introduction, integrating peak areas value is measured, is horizontal by ordinate Y, ginsenoside Re's concentration X of integrating peak areas value Coordinate draws standard curve, the results showed that, equation of linear regression is：Y=0.3871X+0.1593, R²=0.9992, i.e. ginseng Saponin(e Re reference substance solutions concentration linear relationship within the scope of 10 μ of μ g/mL~100 g/mL is good.

1.3.3 ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes the assay research of polysaccharide component in powder extracts

Phenol-sulfuric acid and colorimetric method measures the content that ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes polysaccharide in powder extracts Aqueous extracts

1.3.3.1 prepared by reference substance solution

DEXTROSE ANHYDROUS reference substance about 0.025g is taken, it is accurately weighed, it sets in 250mL measuring bottles, adds appropriate water dissolution, be diluted to Scale shakes up, and the glucose standards solution containing 0.1004mg/mL is made.

1.3.3.2 ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes the preparation of powder extracts test solution

Precision pipettes water obtained under each process conditions and puies forward sample liquid (being approximately equivalent to crude drug amount 0.5g), and add diethyl ether 100mL It is heated to reflux 1 hour, stands, let cool, carefully discard ether solution, residue sets water-bath Back stroke and uses up ether.80% ethyl alcohol is added 100mL is heated to reflux 1 hour, filters while hot, and filter residue washs by several times with filter 80% ethyl alcohol 30mL of heat, and filter residue is together with filter paper It sets in flask, adds water 150mL, be heated to reflux 2 hours.It filters while hot, with a small amount of hot water washing nozzle, merging filtrate and washing lotion, It lets cool, moves in 250mL measuring bottles, be diluted with water to scale, shake up, obtain test sample 1；It draws 2mL test samples 1 and sets 10mL test tubes In, graduation mark is added water to, test sample 2 is obtained.Precision draws 1mL test samples 2, and 1mL distilled water, the phenol of 1mL5%, 5mL is added The concentrated sulfuric acid shakes up, and room temperature sets 10min, and 40 DEG C of heating water bath 15min are cooled to room temperature, and extinction is measured in maximum absorption wave strong point Spend A.

1.3.3.3 the selection of maximum absorption wavelength

Precision draws glucose control product solution and ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts water and carries sample solution 1mL, according to 1.3.2 method carries out full wavelength scanner respectively within the scope of 400nm-600nm under item, as a result comparison liquid and test liquid absorption maximum Wavelength selects 490nm to measure wavelength all in 490nm.

1.3.3.4 the drafting of standard curve

Precision draws DEXTROSE ANHYDROUS reference substance solution 0mL, 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL, sets respectively In 10mL tool plug test tubes, respectively plus distilled water is mended to 2.0mL, respectively accurate again that 5% phenol solution 1mL is added, and is shaken up, rapid to add Enter the 5mL concentrated sulfuric acids, shake up, be placed at room temperature for 10min, set 40 DEG C of heating water bath 15min, takes out, room temperature is rapidly cooled to, with corresponding Reagent is blank, and absorbance is measured in the maximum absorption wave strong point of 490nm.DEXTROSE ANHYDROUS solution concentration is carried out with absorbance Linear regression obtains linear equation：Y=0.017176+0.043539X, R=0.9929.

1.4 extraction process by water research methods and result

1.4.1 ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts water boiling and extraction basic skills

Add a certain amount of water to impregnate 2h in the 9 taste Chinese medicine such as ginseng, Poria cocos, the fruit of Chinese wolfberry, sealwort, Chinese yam, impregnates certain time, add Certain times of amount water, decocts certain time, is during which stirred continuously, decocted 2 times or 3 times with method, filtering, merging filtrate is settled to one Determine volume.

1.4.2 ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts water and proposes number investigation

Three parts of the 9 taste Chinese medicine such as ginseng, Poria cocos, the fruit of Chinese wolfberry, sealwort, Chinese yam is taken, 100 grams every part, impregnates, is set by table 1 respectively Water carries parameter in meter scheme respectively, prepares three parts of Aqueous extracts, 50 DEG C are concentrated under reduced pressure into 100mL, are processed into test liquid respectively, often Part by 1.3.3 lower measurement polyoses contents, 1 is the results are shown in Table, as seen from the results in Table 1, water, which puies forward 2 Polyose extraction amounts and accounts for water, to be carried three times 94% or more, Gu Shui carry 2 times and be preferred.

The ginseng Siberian cocklebur paddy perfume (or spice) of table 1 reconstitutes powder extracts water and carries number investigation design table

1.4.3 response surface design experiment method preferably joins Siberian cocklebur paddy perfume (or spice) and reconstitutes powder extracts extraction process by water parameter

(1) design and experimental method of factor level table

On the basis of trial test, powder extracts water is reconstituted to ginseng Siberian cocklebur paddy perfume (or spice) and carries key process parameter, i.e., water temperature raising degree plus Water, water put forward the response surface design experiment method for the time using Three factors-levels Box-Bebnken experimental designs, pass through Design 8.0 softwares of Expert carry out regression analysis to experimental data.With polyoses content in medicinal material (%) for evaluation index value, screening is most Excellent water carries technological parameter, and each factor level is shown in Table 2.

The ginseng Siberian cocklebur paddy perfume (or spice) of table 2 reconstitutes powder extracts water and carries Box-Behnken design experiments factor level and coding schedule

Note：When amount of water is 10 times of medicinal material amount, for the first time plus 6 times of amounts of water, second plus 4 times of amounts of water；Amount of water is medicinal material When measuring 20 times, for the first time plus 12 times of water is measured, adds 8 times of amounts of water for second；When amount of water is 30 times of medicinal material amount, for the first time plus 18 times of water Amount, second plus 12 times of amounts of water.Water carry the time be 1.0h when, first time 0.6h, second of 0.4h；Water carry the time be 2.0h when, First time 1.2h, second of 0.8h；Water carry the time be 3.0h when, first time 2.0h, second of 1.0h.

(2) ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts water and puies forward response surface experiments arrangement and result

Response surface experiments arrangement and the results are shown in Table 3, totally 14 testing sites of table 3, wherein 12 are factorial point, 2 are zero, Factorial point is carried the vertex that the time (C) constitutes by independent variable value water temperature raising degree (A), amount of water (B) and water, and zero is region The experiment of central point, wherein zero is repeated 2 times, to estimating test error.

The ginseng Siberian cocklebur paddy perfume (or spice) of table 3 reconstitutes powder extracts water and puies forward response surface design design experiment measurement result

(3) foundation of model and significance analysis

Quadratic Regression Fitting is carried out to 3 test data of table using Design Expert8.0 softwares, obtains Y value to water temperature raising degree (A), the secondary multinomial regression model equation that amount of water (B) and water carry the time (C) is：Y=2.66+0.73 × A+0.19 × B+ 7.062E-003×C+0.11×A×B-0.069×A×C+0.054×B×C+0.35×A²- 0.51×B²+0.40×C², Significance test is carried out to the model, the results are shown in Table 4.

4 regression model variance analysis table of table

The model P values < 0.05 that experiment is selected has conspicuousness, loses and intends item not significantly (P=0.1209 > 0.05), explanation The model has statistical significance；Coefficient of multiple correlation R²Adj=0.9524 illustrates that the model can explain 95.24% response Variation, thus the fitting degree of the model is relatively good, can analyze and predict that ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts with this model Decocting technological parameter.

(4) ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes Surface Analysis result of each factor of powder extracts decocting technique to reinforcement exponential effect

Powder extracts decocting process data is reconstituted using 8.0 softwares of Design Expert to ginseng Siberian cocklebur paddy perfume (or spice) handle To Surface Analysis as a result, it can also be seen that, notable factor is influenced in first order is from the further inspection of regression equation items variance Water temperature raising degree, amount of water sort within the scope of selected each factor level according to the influence to result:That is water temperature raising degree > adds Water > water carries the time, in three factors, each reciprocation unobvious between factor two-by-two.

(5) ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts decocting optimization of process conditions result

Equation Y is solved using 8.0 softwares of Design-Expert, ginseng Siberian cocklebur paddy perfume (or spice) is obtained and reconstitutes powder extracts decocting most Excellent technological parameter is：Water temperature raising degree is 100 DEG C, and amount of water is 22.43 times, and water carries the time as 3.00h, and theoretically Y value is 4.239%.

(6) verification test result

The obtained optimal conditions of powder extracts extraction process by water are reconstituted to ginseng Siberian cocklebur paddy perfume (or spice) to be finely adjusted：Water temperature raising degree is 100 DEG C, it is 12 times to extract amount of water for the first time, and it is 2.00h that water, which carries the time, and second to extract amount of water be 10 times, and water carries the time and is 1.00h.Three batches of actually measured Y values of lab scale verification test are 4.213%, RSD=2.92% (n=3), are close with theoretical value, So it is accurate and reliable to carry Extraction technique based on the optimization water obtained by Response Surface Method.

2 ginseng Siberian cocklebur paddy perfume (or spice) reconstitute powder extracts low temperature spray drying technical study

Polysaccharide and ginsenoside R1, Rg1, Rb1 are all dissolved in water, therefore process route preferably uses water as solvent to extract, through filtering, After concentration, centrifugation, then by its low temperature spray drying at dry extract.Early period, preliminary result showed low temperature spray drying condition The factors such as inlet air temperature, pump medicine speed, wind turbine rate reconstitute powder extracts dry extract yield, polysaccharide and ginseng to ginseng Siberian cocklebur paddy perfume (or spice) Saponin(e R1, Rg1, the Rb1 rate of transform is affected, therefore this experiment reconstitutes powder extracts dry extract to join Siberian cocklebur paddy perfume (or spice) and must measure to comment Valence index optimizes ginseng Siberian cocklebur paddy perfume (or spice) using response surface design experiment method and reconstitutes powder extracts low temperature spray drying technological parameter, is work The production of industry metaplasia provides foundation.

2.1 instruments and material

The medicinal materials such as ginseng (Jilin), Poria cocos, Chinese yam, the fruit of Chinese wolfberry (Yunnan) are purchased from the big pharmacy of common people, and (Hunan is new for sealwort Hua Lvyuan agriculture and forestry science and technologies Co., Ltd), meet 2015 editions through pharmaceutical college of Hunan University of Traditional Chinese Medicine Sunday treasured professor identification《Middle traditional Chinese medicines Allusion quotation》One pertinent regulations；The laboratories YC-1800 low temperature spray drying machine (the refined journey experimental instruments and equipment limited in Shanghai)； ME204E Type electronic analytical balance (Shanghai plum Teller-support benefit Instrument Ltd.)；TCS-100 types electronic platform scale (Shanghai Qian Feng electronics Instrument Ltd.)；Rotary evaporator RE-2000B (Yuhua Instrument Co., Ltd., Gongyi City)；III circulating water types of SHB- are more With vacuum pump (Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd.)；MC densitometers (state-run Hebei province Wuqiang County essence creates instrument and meter factory)；In Prescription decocts machine popular style-trendy (Changsha Zhuo is at Medical Devices Co., Ltd.)；；PW135 types crusher for Chinese herbal medicine (Tianjin it is safe this Special Instrument Ltd.)；2X2-2 types sliding vane rotary vacuum pump (Taizhou of Zhejiang refinement vacuum pump Co., Ltd).

2.2 basic skills

2.2.1 response phase method Optimum Experiment designs

Table 5 joins Siberian cocklebur paddy perfume (or spice) and reconstitutes powder extracts low temperature spray drying Box-Behnken experimental factors level and coding schedule

According to low temperature spray drying technique single factor test trial test as a result, utilizing the center in Design-Expert8.0 softwares Composite test design principle, it is investigation factor (independent variable) to choose inlet air temperature, fan speed, pump medicine speed, with cold nebulization It is inspection target (response) that dry dry extract, which obtains magnitude, and concentrate relative density is 1.07g/mL, and atomizing pressure is 0.2Mpa carries out Three factors-levels response phase method design experiment and advanced optimizes low temperature spray drying technological parameter, factor water Flat design and coding are shown in Table 5.

(1) arrangement of response surface design experiment and result table

The arrangement of response surface design experiment and it the results are shown in Table 2, totally 13 testing sites of table 2, wherein 12 are factorial point, 2 are Zero, factorial point is by the top that independent variable value inlet air temperature (A), fan speed (B) and pump medicine speed (C) are that independent variable is constituted Point, zero are the central point in region, and wherein zero experiment is repeated 2 times, to estimating test error.

Table 6 joins Siberian cocklebur paddy perfume (or spice) and reconstitutes powder extracts low temperature spray drying Box-Behnken experiment measurement results

Note：Y value computational methods：Dry extract must be measured

(2) foundation of model and significance analysis

Quadratic Regression Fitting is carried out to 6 test data of table using 8.0 softwares of Design Expert, obtains Y value into wind-warm syndrome Degree (A), fan speed (B) and pump medicine speed (C) secondary multinomial regression model equation be：

Y value=6.9-0.8875 × inlet air temperature+0.9 × fan speed -1.0375 × pump medicine speed -0.5 × into wind-warm syndrome Degree × fan speed+0.825 × inlet air temperature × -0.1 × fan speed of pump medicine speed × pump medicine speed -0.3375 × into Air temperature²+ 0.4875 × fan speed²- 0.2875 × pump medicine speed², significance test is carried out to the model, the results are shown in Table 7.

7 regression model variance analysis table of table

Source	Quadratic sum	Degree of freedom	It is square	F values	P values
						Model	26.8963	9	2.9885	11.23187	0.0022
A- inlet air temperature	6.3012	1	6.3012	23.68255	0.0018
						B- fan speeds	6.48	1	6.48	24.35436	0.0017
C- pumps medicine speed	8.6112	1	8.6112	32.36443	0.0007
						AB	1	1	1	3.758389	0.0937
AC	2.7225	1	2.7225	10.23221	0.0151
						BC	0.04	1	0.04	0.150336	0.7097
A²	0.4796	1	0.4796	1.802543	0.2213
						B²	1.0006	1	1.0006	3.760862	0.0936
C²	0.3480	1	0.3480	1.308018	0.2904
						Residual error	1.8625	7	0.2661
It loses quasi-	1.8625	3	0.6208
						Error	0	4	0
It is comprehensive	28.7588	16
						Correction coefficient	R²=	0.9352	R²Adj=	0.8520

The model P=0.0022 that experiment is selected has conspicuousness, loses and intends item not significantly (P=0.6208 > 0.05), explanation The model has statistical significance；Coefficient of multiple correlation R²Adj=0.8520 illustrates that the model can explain the change of 85.2% response Change, thus the fitting degree of the model is relatively good, can analyze and predict herbal cuisine ginseng Siberian cocklebur paddy perfume (or spice) brewed powder extraction with this model Object low temperature spray drying technological parameter.

(3) ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes Surface Analysis of each factor of powder extracts low temperature spray drying technique to reinforcement exponential effect As a result

Powder extracts low temperature spray drying technique number is reconstituted to herbal cuisine ginseng Siberian cocklebur paddy perfume (or spice) using 8.0 softwares of Design Expert According to being handled, from the further inspection of regression equation items variance, it can also be seen that, it is pump medicine that notable factor is influenced in first order Speed, fan speed, inlet air temperature sort within the scope of selected each factor level according to the influence to result:C > B > A, Medicine speed > fan speed > inlet air temperature is pumped, in 3 factors, inlet air temperature and fan speed, fan speed and pump medicine speed Reciprocation is not notable between spending factor two-by-two, significant interaction between inlet air temperature and pump medicine speed factor.

2.3 experimental result

2.3.1 ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts low temperature spray drying optimization of process conditions result

Equation Y value is solved using 8.0 softwares of Design-Expert, obtains integration of drinking and medicinal herbs herbal cuisine ginseng Siberian cocklebur paddy perfume (or spice) brewed powder Extract low temperature spray drying optimal procedure parameters are：Inlet air temperature is 100-150 DEG C, and fan speed is 10-30m³/ min, Pump medicine speed is 5-20 (ml/min), and atomizing pressure is 0.2-0.4Mpa, and theoretically Y value is 11.01%.

3. joining research and the process optimization of Siberian cocklebur paddy perfume (or spice) brewed powder

3.1. technological process

Raw material identification (should meet corresponding food standard and pertinent regulations) --- weigh --- water immersion --- water carry, mistake Filter --- merges, concentration --- spray drying --- obtains dry extract

--- removing sand, --- --- microwave ripening is --- cooling --- to crush --- sieving with 100 mesh sieve --- for cleaning for cereal materials Obtain grain dust

Amount (8 parts)+each grain dust of fixed dry extract in proportion+additive kind ingredient --- more people's subjective appreciations --- Select --- detection --- sterilizing --- packing of mouthfeel color and luster optimum formula

Using single factor experiment and orthogonal test, the allotment formula of research ginseng Siberian cocklebur paddy perfume (or spice) brewed powder.By lotus seeds, coix seed, Bitter buckwheat, black soya bean, black rice, polished rice are by microwave ripening, and after cooling, crushing sieves with 100 mesh sieve.First by preliminary experiment determine bitter buckwheat, The addition range of black soya bean, black rice, polished rice does grade by adding proportion, determines basis cereal formula, then utilizes experiment of single factor Study kudzu-vine root powder, konjaku flour, whole milk powder, the additive amounts such as xylitol to join Siberian cocklebur paddy perfume (or spice) brewed powder organoleptic quality affecting laws and After substantially adding value range, positive quadraturing design test is recycled to find out the best allotment formula of ginseng Siberian cocklebur paddy perfume (or spice) brewed powder organoleptic quality.

3.2 ginseng Siberian cocklebur paddy perfume (or spice) brewed powder basis cereal formula composition designs

Ginseng Siberian cocklebur paddy perfume (or spice) is reconstituted into powder extracts and is fixed as 8 parts, since lotus seeds, coix seed content of starch are high, nutritive value is high, It is good in taste, therefore it is extractd from ginseng Siberian cocklebur invigorating the spleen formula powder, the basic cereal materials as cereal brewed powder are directly used in, And the two is fixed as 6.6 parts (3.3 parts of lotus seeds, 3.3 parts of coix seeds), then remaining cereal bitter buckwheat, black soya bean, black rice, polished rice totally 20 Part.By pressing each material 1：2：3 ratio is allocated and influence to appearance, mouthfeel, brew carries out ranking consistence inspection, According to table 1, by good extremely poor hierarchical arrangement of pressing at 9~1, best person is set to 9, and worst person is set to 1, the results are shown in Table 8.

Influence (m=3, n=9) of the 8. material rate score of table to pulvis quality

A represents bitter buckwheat；B represents black soya bean；C represents black rice；D represents polished rice.1,2,3 represent each raw material in this Proportion.

By in table 8, appearance, mouthfeel, the grading system of brew and square (R_i ²) following formula is substituted into, calculate S=∑s R_i ²- (∑R_i)²/ n and x²=12S/mn (n+1).Wherein, m=3, n=9.The result shows that S=468, x²=20.8.According to v= 8, table look-up to obtain x² _0.05(8)=15.507, it is known that x²>x² _0.05(8), show P<0.05, prompt inspection result to have statistical significance, i.e., each original Material ratio is to appearance, mouthfeel, the significant difference of brew.It is evaluated according to aggregation of data in table 1, A₂:B₂:C₃:D₁Preferably, i.e., More excellent composition of raw materials group becomes 5 parts of bitter buckwheat, 5 parts of black soya bean, 7.5 parts of black rice, 2.5 parts of polished rice, 3.3 parts of lotus seeds, 3.3 parts of coix seed.

3.2 auxiliary material optimal proportion single factor experiments design

In single factor experiment, addition different quality konjaku flour, kudzu-vine root powder, whole milk powder, xylitol are investigated respectively to joining Siberian cocklebur The influence of paddy perfume (or spice) brewed powder quality.The additive amount of four kinds of ingredients is respectively set to suitable gradient, if variation investigate because Element, other substance additive amounts select the optimal additive amount of each component as definite value.Konjaku flour, kudzu-vine root powder, full-cream is investigated successively Influence of the additive amount to the product sensory quality of milk powder, xylitol.

3.2.1 join the sensory evaluation scores standard of Siberian cocklebur paddy perfume (or spice) brewed powder

The sensory evaluation scores standard is shown in Table 9.

3.2.2 join Siberian cocklebur paddy perfume (or spice) brewed powder sensory evaluation standard

20 syndics are selected, analytic type subjective appreciation is carried out to ginseng Siberian cocklebur paddy perfume (or spice) brewed powder.Evaluation project has appearance (A), wind Taste (B), mouthfeel (C) and brew (D), projects total score are respectively 100 points, and standards of grading are shown in Table 3, and sensory evaluation scores (S)= 0.1A+0.3B+0.4C+0.2D。

3.3 results and analysis

3.3.1 join the determination of Siberian cocklebur paddy perfume (or spice) brewed powder optimization formula

On the basis of single factor experiment result, join kudzu-vine root powder in Siberian cocklebur paddy perfume (or spice) brewed powder, konjaku flour, complete for further research The optimum addition of fat milk powder, xylitol, finds out the more excellent formula of ginseng Siberian cocklebur paddy perfume (or spice) brewed powder, and positive quadraturing design test is selected in experiment Method, using konjaku flour, kudzu-vine root powder, whole milk powder, xylitol additive amount as four optimizing factors, to join Siberian cocklebur paddy perfume (or spice) brewed powder Sensory evaluation scores be that index carries out four factors three horizontal L9 (3⁴) orthogonal test research.

Positive quadraturing design test factor is shown in Table 10 with level design, and range analysis of orthogonal experiment the results are shown in Table 11, orthogonal test The results of analysis of variance is shown in Table 12.

Table 10 L9 (34) positive quadraturing design test factor and level design

11 positive quadraturing design test arrangement of table and experimental result table

The results of analysis of variance between 12 orthogonal test factor of table

Soruces of variation	Quadratic sum	Degree of freedom	It is square	F values	P values
						A	2.8889	2	1.4444
B	90.8889	2	45.4444	31.4615	0.0308
						C	176.2222	2	88.1111	61.0000	0.0161
D	944.8889	2	472.4444	327.0769	0.0030
						Error	2.8889	2	1.4444
Summation	1214.8889

By range analysis result table 11 it is found that when joining Siberian cocklebur paddy perfume (or spice) brewed powder sensory evaluation scores as inspection target, each factor is made It is D with primary and secondary>C>B>A, it can be seen that, xylitol is to influence the most important factor of the brewed powder, followed by whole milk powder, pueraria lobata Powder, and factor one of of the konjaku flour as orthonormal design of experiments, it is not statistically significant, it is contemplated that product cost, therefore select minimum. Further according to K values analyzed, obtains A₁>A₂>A₃, B₃>B₁>B₂, C₂>C₁>C₃, D₃>D₂>D₁。

12 are shown in Table it is found that the A (konjaku flour) in ginseng Siberian cocklebur paddy perfume (or spice) brewed powder is whole to product without statistics by the results of analysis of variance Meaning is learned, B (kudzu-vine root powder), C (whole milk powder) have conspicuousness to whole sensory evaluation scores, and D (xylitol) is to whole sense organ Scoring has pole conspicuousness.This shows that kudzu-vine root powder, whole milk powder, xylitol have important work to the organoleptic quality of the brewed powder With.

3.4.1 join the verification of the more excellent formula of Siberian cocklebur paddy perfume (or spice) brewed powder

On the basis of above-mentioned orthogonal test, the more excellent formula to joining Siberian cocklebur paddy perfume (or spice) brewed powder makees further verification, verification examination The average value for testing sensory evaluation scores is above orthogonal test maximum value, therefore illustrates the sense organ that the formula that orthogonal test determines is embodied Stay in grade is feasible.

Optimum formula verification test the results are shown in Table 13.

13. optimum formula verification test result of table

3.4.2 the suboptimization again of more excellent formula

To carrying out adjusting and optimizing in the brewed powder color and luster, mouthfeel on the basis of above-mentioned more excellent formula, purple sweet potato powder, red is utilized Date powder additive amount carries out single factor experiment adjustment to product, the results are shown in Table 14.

14. final optimization pass of table adjusts result table

According to upper table as a result, on the basis of considering further that products material cost, 2 parts of purple sweet potato powder additive amount is selected, red date powder adds It is best that 1.3 parts of dosage can reach mouthfeel.

4. ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts toxicologic study

This research gives given the test agent using ICR mouse oral gavages, is observed continuously 14 days, is tried from the acute toxicity of mouse It tests result and whether understands given the test agent with toxicity and toxic reaction degree and the organ being related to, be further toxicity test Dose design, toxic reaction index selection and clinical test development provide experimental basis.

4.1 experiment material

(1) given the test agent

Ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts, lot number：20180123, brown solid powder (contains particle), and every gram of sample is suitable In crude drug 10.70g.Adult recommends amount 6.7g/ times, and 2 times/day, boiling water is taken after mixing it with water, and is provided by Hunan University of Traditional Chinese Medicine, protects It is stored in low temperature drying environment.

(2) experimental animal

SPF grades of ICR mouse 20, half male and half female, average weight：19.9g, 18.7~21.0g of weight range are purchased from Hunan This Lake scape reaches experimental animal Co., Ltd, experimental animal production licence number：SCXK (Hunan) 2016-0002, experimental animal matter Measure quality certification number：No.43004700043125 raises (temperature in the areas barrier environment C of Drug Safety Evaluation Center of Hunan Province Range：20.0~26.0 DEG C；RH range：40.0%~70.0%), experimental animal uses credit number：SYXK (Hunan) 2015-0016.Mouse feed by Beijing Australia of section pull together feed corporation,Ltd provide, credit number：SCXK (capital) 2014-0010 is raised Expect quality certification number：No.11002900035009.

(3) key instrument

PL2002 type electronic balances, Mettler-Toledo Instrument (Shanghai) Co., Ltd.；MS105DU type analysis balances, plum Co., Ltd in Teller-support benefit.

4.2 experimental method

4.2.1 animal welfare and protection

This experiment examines through center management of laboratory animal (ethics) committee, implements after approval, and it is related real to press country completely The regulation for testing animal welfare regulation and this center relevant criterion operating instruction executes.

4.2.2 the foundation of experimental design

Preliminary result：Ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes that powder extracts are soluble easily in water, and configurable maximum is to sample concentration >=0.50g/ ML, therefore use in for 24 hours and repeatedly tested to sample method, ICR mouse give ginseng Siberian cocklebur paddy perfume (or spice) brewed powder by the oral gavages of 20mL/kg and carry Object is taken, altogether twice to sample, 20g/kg (90 times that are equivalent to human body recommendation amount) is amounted to sample dosage, is not observed to after sample There are the abnormal conditions such as death to animal.

4.2.3 experiment grouping and dose design

According to preliminary result, this experiment carries out formal test using maximum to sample amount method, chooses quarantine qualification ICR mouse 20, after animal is deprived of food but not water 6h, given the test agent is given by the oral gavages of 20mL/kg for half male and half female, interior for 24 hours to give sample two It is secondary, give sample interval 6h, ginseng Siberian cocklebur paddy perfume (or spice) to reconstitute powder extracts and amount to 20g/kg to sample dosage every time.

4.2.4 Testing index

(1) general clinical observation：To examined in 0~4h after sample and record each group animal whether occur toxic reaction with And toxic reaction symptom, time of occurrence and death condition etc. then carry out gross anatomy observation, surviving animals such as animal dead Hereafter it is observed continuously 14 days；

(2) weight：To on the day of sample and to the 7th day after sample, carry out animal within the 14th day and weigh；

(3) gross anatomy is observed：At the end of observation, all surviving animals are put to death, carry out gross anatomy observation, as internal organs are sent out Now apparent lesion then carries out histopathological examination.

4.2.5 experimental result

(1) general clinical observation

It is observed continuously 14 days in 0~4h on the day of sample and after administration：Reaction, experimental period are shown no obvious abnormalities to sample group mouse Do not occur animal dead situation inside.

(2) weight

As shown in Table 15：To before sample and to the 7th day after sample, weigh within the 14th day, mouse weight increases and weight increases in normal It focuses in normal range (NR).

15 mouse weight result of table

(3) gross anatomy is observed

Last time puts to death all survival mices after observing and carries out gross anatomy inspection, visually observes the position of internal organs Set, size, color and luster, adhesion, surface and section quality etc., be showed no the abnormal changes such as hydrops and tumour.

4.3 experiment conclusion

Under this experiment condition, mouse oral gavage gives ginseng Siberian cocklebur paddy perfume (or spice) and reconstitutes powder extracts, has no overt toxicity reaction Dosage (NOAEL) >=20g/kg (is equivalent to 90 times that human body recommends amount).

Ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes inhibiting effect of the powder extracts to Human Gastric Cancer Cells In Vitro MGC803

Observe that ginseng Siberian cocklebur paddy perfume (or spice) reconstitutes powder extracts and has to human gastric cancer epithelial cell MGC803 growing multiplications by CCK-8 methods Certain inhibiting effect as a result may be more acurrate, more stable with increasing for experiment number.

Claims

1. a kind of nutrition brewed powder that Cancer related fatigue is mitigated or eliminated, which is characterized in that press mass parts, the nutrition brewed powder It is made of following raw material：

Chinese medicine constituents：1-5 parts of ginseng, 5-10 parts of Poria cocos, 4-8 parts of Semen Lablab Album, 5-10 parts of Chinese yam, 4-9 parts of lotus seeds, dried orange peel 1-5 Part, 4-9 parts of coix seed, 5-10 parts of the fruit of Chinese wolfberry, 5-10 parts of sealwort；

Additive kind ingredient：1-6 parts of kudzu-vine root powder, 1.5-3 parts of konjaku flour, 0.5-3 parts of whole milk powder, 1-8 parts of xylitol, purple sweet potato powder 1-5 parts, 1-4 parts of red date powder.

2. nutrition brewed powder as described in claim 1, which is characterized in that the nutrition brewed powder is made of following raw material：

Chinese medicine constituents：4.7 parts of ginseng, 8.7 parts of Poria cocos, 6.7 parts of Semen Lablab Album, 8.7 parts of Chinese yam, 6.7 parts of lotus seeds, 3.3 parts of dried orange peel, 6.7 parts of coix seed, 8.7 parts of the fruit of Chinese wolfberry, 8.7 parts of sealwort；

Additive kind ingredient：4.7 parts of kudzu-vine root powder, 0.3 part of konjaku flour, 1.7 parts of whole milk powder, 5.3 parts of xylitol, 2 parts of purple sweet potato powder, 1.3 parts of red date powder.

3. the preparation method of nutrition brewed powder as claimed in claim 1 or 2, which is characterized in that include the following steps：

It decocts, filters after Chinese medicine constituents are impregnated, after filtrate is condensed into medicinal extract, extract powder is made in spray drying；By five cereals Ingredient cures, and crushes, five flours are made；Extract powder, five flours and additive kind components in certain proportion are mixed, nutrition is obtained and reconstitutes Powder.

4. the preparation method of nutrition brewed powder as claimed in claim 1 or 2, which is characterized in that include the following steps：By ginseng, Semen Lablab Album, dried orange peel, Chinese yam, sealwort crushed 10 mesh sieve, and Poria cocos, matrimony vine do not crush, and above seven taste decocts after immersion, filter, filter After liquid is condensed into medicinal extract, extract powder is made in spray drying；It by lotus seeds, coix seed, the curing of five cereals constituents, crushes, five cereals is made Powder；Extract powder, five flours and additive kind components in certain proportion are mixed, nutrition brewed powder is obtained.

5. preparation method as described in claim 3 or 4, which is characterized in that after adding maltodextrin in medicinal extract, then sprayed Mist is dried.

6. preparation method as described in claim 3 or 4, which is characterized in that it is 2 times to decoct number, and first time amount of water is medicine 16 times of material amount, water decoct time 2 h；2nd amount of water is 10 times of quality of medicinal material, water decocting time 1 hour.