CN108719989A - A kind of amino acid pattern containing pueraria lobata relieves the effect of alcohol ferment - Google Patents
A kind of amino acid pattern containing pueraria lobata relieves the effect of alcohol ferment Download PDFInfo
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- 150000001413 amino acids Chemical class 0.000 title abstract description 10
- 241000219781 Pueraria montana var. lobata Species 0.000 title abstract 3
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- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to ferment field, especially a kind of amino acid pattern containing pueraria lobata relieves the effect of alcohol ferment.A kind of amino acid pattern containing the pueraria lobata ferment that relieves the effect of alcohol is formed through probiotics fermention after being compounded by integration of drinking and medicinal herbs raw material, fruits and vegetables, five cereals, functional amino and auxiliary material.Preparation method is that integration of drinking and medicinal herbs raw material, five cereals and fruits and vegetables are crushed or compounded after being beaten, enzymolysis processing is carried out with lemon acid for adjusting pH value addition complex enzyme, then composite probiotics ferment processing is inoculated with after supplement formula, again through after-ripening, clarifying treatment, and then functional auxiliary material is added, filling and bus sterilization processing is made.The present invention according to different type raw material relieve the effect of alcohol, in terms of protect liver the effect of the scientific design that is formulated of mechanism, merge efficient cryogenic complex enzyme hydrolysis technique, the active material being effectively retained comprehensively in raw material, and then fermentation process is carried out using preferred beneficial bacteria of intestinal tract, convert part macromolecular substances to the small molecule conducive to absorption of human body, and by probiotics promote intestinal absorption, adjust enteron aisle balance, discharge enterotoxin etc. complements each other the effect of, it is whole improve product relieve the effect of alcohol, in terms of protect liver the effect of.
Description
Technical field
The present invention relates to ferment field of relieving the effect of alcohol, especially a kind of amino acid pattern containing pueraria lobata relieves the effect of alcohol ferment.
Background technology
Pueraria lobata is that a kind of happiness is born in the thick grass of hillside or the perennial liana in more dark and damp place, contain a variety of flavonoids at
Point, main active is daidzein, daidzin, Puerarin etc., and the power of regeneration of liver cell can be improved, and restores normal liver machine
Can, it boosts metabolism, reinforces liver detoxification function, prevent damage of the alcohol to liver；Simultaneously to improving angiocardiopathy
There are certain treatment and health-care effect.
Fruits and vegetables such as sugarcane, pears, lotus root and ternip moisturize tonifying Qi, and five cereals such as black soya bean, soya bean, mung bean, green soya bean and rde bean can solve
Wine poison, only polydipsia, integration of drinking and medicinal herbs raw material include that pueraria lobata, Radix Salviae Miltiorrhizae, hawthorn, flower of kudzuvine, dried orange peel and ginseng, Radix Glycyrrhizae and peppermint are even more to have
The effect of refresh oneself, born fluid, disappear fatigue, liver protecting, shield taste.
Functional amino is such as：Glutamine, alanine, leucine, lazy propylhomoserin can promote intestinal growth and mucous membrane to repair
It is multiple, human body protein metabolism is participated in, the conversion of vivo protein amino acid is accelerated, to protecting liver, improves myocardial function
Important function again.
Lactic acid that probiotics generates, propionic acid can it is effective in enteron aisle in and ethyl alcohol, reduce injury of the alcohol to human body；Together
When, probiotics can promote absorption of the human body to above-mentioned function raw material in enteron aisle.Through biological enzymolysis and probiotics fermention, after-ripening
After be re-dubbed functional relieving alcoholism and protecting liver ferment so that each ingredient synergistic effect helps to alleviate excessive drinking and be caused to body
Damage.
Invention content
It relieves the effect of alcohol ferment the object of the present invention is to provide a kind of amino acid pattern containing pueraria lobata, technical solution is realized in
's：
A kind of the relieve the effect of alcohol raw material of ferment of the amino acid pattern containing pueraria lobata includes mainly the raw material of integration of drinking and medicinal herbs, fruit and vegetable, five cereals
Group food, functional amino and compounding raw material.
The raw material of the integration of drinking and medicinal herbs includes mainly：Pueraria lobata, Radix Salviae Miltiorrhizae, hawthorn, flower of kudzuvine, dried orange peel and ginseng, Radix Glycyrrhizae and thin
Lotus.
Preferably, pueraria lobata is 10 ~ 15wt% of the raw material gross weight, Radix Salviae Miltiorrhizae is 1 ~ 5wt% of the raw material gross weight, mountain
Short, bristly hair or beard is 1 ~ 5wt% of the raw material gross weight, flower of kudzuvine is 3 ~ 8wt% of the raw material gross weight, dried orange peel is the raw material gross weight
1 ~ 5wt%.Ginseng, Radix Glycyrrhizae and peppermint in other Chinese herbal medicines that can relieve the effect of alcohol respectively account for 1 ~ 3wt% of the raw material gross weight.
The fruit and vegetable includes mainly：Sugarcane, pears, lotus root and ternip.
Preferably, sugarcane is 1 ~ 5wt% of the raw material gross weight, pears are 3 ~ 8wt% of the raw material gross weight, lotus root is the original
Expect 1 ~ 5wt% of total weight, 1 ~ 5wt% that ternip is the raw material gross weight.
The five cereals group food includes mainly：Black soya bean, soya bean, mung bean, green soya bean and rde bean.
Preferably, above-mentioned five cereals group food equal proportion addition, accounts for 5 ~ 10wt% of the raw material gross weight altogether.
The functional amino includes mainly：Glutamine, alanine, leucine, lazy propylhomoserin.
Preferably, glutamine be functional amino total weight 50wt%, alanine be functional amino gross weight
The 20wt% of amount, the 15wt% that leucine is functional amino total weight, lazy propylhomoserin be functional amino total weight
15wt%.Preferably, functional amino is 0.5 ~ 1wt% of the raw material gross weight.
The compounding raw material includes mainly：Honey, white granulated sugar, hydroxyl isomaltulose and yeast powder.
It is preferred 1 ~ 5wt% that honey is 2 ~ 10wt% of the raw material gross weight, white granulated sugar is the raw material gross weight, different
1 ~ 3wt% that maltitol is 0.5 ~ 1wt% of the raw material gross weight and yeast powder is the raw material gross weight
Second object of the present invention is to provide a kind of amino acid pattern containing pueraria lobata and relieves the effect of alcohol ferment, and technical solution is realized in
's：
Step 1. pre-processes.The integration of drinking and medicinal herbs raw material, five cereals raw material are crushed to 40 ~ 100 mesh, after fruit and vegetable materials mashing,
It is uniformly mixed, moisturizing is used in combination citric acid adjusting to make pH=4.0 ~ 6.0 to 100 parts.
Step 2. digests.Ratio according to 1 ~ 3 ‰ in solution obtained by step 1 is accessed into a variety of enzymes, carries out complex enzyme
Solution, 30 ~ 60 DEG C of enzymolysis process digest 10 ~ 60Min.
As described in step 2, it is characterised in that complex enzyme hydrolysis process choice two or more enzyme preparation below：It is acid
Amylase, acid pectase, acidic cellulase, acid protease.
Step 3. compounds.White granulated sugar, 2 ~ 5% ratio is added according to 5 ~ 10% ratio into the enzymolysis liquid obtained by step 2
Yeast powder is added, is uniformly mixed, 90 ~ 100 DEG C, sterilize 15 ~ 30min.
Step 4. after-ripening.Into the combination solution obtained by step 3 compound probiotic is accessed according to 3 ~ 5% ratios, 15 ~ 25
DEG C, after-ripening 3 ~ 6 months.
As described in step 4, it is characterised in that compound probiotic includes following three kinds or more：Streptococcus thermophilus is protected
Add Leah lactobacillus, lactobacillus plantarum, Lactobacillus casei, bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus rhamnosus, acetic acid
Bacillus, brewer's wort fermented acetic acid bacterium, speed make acetic acid bacteria, acetobacter xylinum, Propionibacterium, enterococcus faecalis, natto withered grass.
Step 5. adds into clear liquid according to 5 ~ 10% ratio through centrifuging or being collected by filtration step 5 acquired solution clear liquid
Enter honey, functional amino is added in 0.5 ~ 1% ratio, and the functional raw materials such as hydroxyl isomaltulose are added in 2 ~ 3% ratio.
Step 6. is filling and sterilizes.Step 6 acquired solution is filling, 75 ~ 80 DEG C of bus sterilization processings 15 ~ 30 are used afterwards
Minute.
Compared with prior art, the new technology that the present invention incorporates, advantage are：
The present invention according to different type raw material relieve the effect of alcohol, in terms of protect liver the effect of the scientific design that is formulated of mechanism, fusion is low
Warm high efficiency composition enzymolysis technique, the active material being effectively retained comprehensively in raw material, and then sent out using preferred beneficial bacteria of intestinal tract
Ferment processing converts part macromolecular substances to the small molecule conducive to absorption of human body, and by probiotics promote intestinal absorption,
Adjust enteron aisle balance, the effect of discharge enterotoxin etc. complements each other, it is whole improve product relieve the effect of alcohol, the work(in terms of protect liver
Effect.
Description of the drawings
Fig. 1 is drunk experiment and the liver section figure of mouselet.
Embodiment 1/2 is to take the mouselet of the ferment of the invention that relieves the effect of alcohol；Comparative example 1/2/3 is not take the mouselet of the present invention.
Field experiment uses healthy mice, gavage to be found to observation after drinking：1. comparative example 1/2/3 is creeps, unstable, hair is loose, closes one's eyes not
Dynamic state of intoxication；Embodiment 1/2 take the present invention relieve the effect of alcohol after ferment activity substantially freely, hair it is more smooth.2. when revival
Between there is notable difference, 1/2 recovery time of embodiment to be shorter than the recovery time of comparative example 1/2/3.Wake states be activity freely, hair
It is smooth and spiritual.Liver ultra-thin section is carried out to comparative example 1/2/3, observation result is；Mitochondria, rough surfaced endoplasmic reticulum (RER), lysosome,
Space of Disse etc. is abnormal change, and has mile abnormality change using 1/2 liver cell of embodiment of the ferment of the invention that relieves the effect of alcohol,
It is significantly improved compared with comparative example group.And long term alcohol gavage handling result is shown, continuously takes the mouse liver slice for the ferment that relieves the effect of alcohol
Display damage, which is significantly less than, does not take.Therefore, a kind of amino acid pattern containing pueraria lobata of the present invention ferment that relieves the effect of alcohol can significantly reduce second
Alcohol has liver certain protective effect to the detrimental effect of liver.
Specific implementation mode
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
Embodiment 1
Step 1. pre-processes.Take pueraria lobata 5wt%, Radix Salviae Miltiorrhizae 4wt%, hawthorn 3wt%, flower of kudzuvine 5wt%, dried orange peel 4wt% and other can relieve the effect of alcohol
Chinese herbal medicine, such as：The integration of drinking and medicinal herbs such as ginseng, Radix Glycyrrhizae and peppermint raw material respectively accounts for the 2wt% and black soya bean, Huang of the raw material gross weight
The five cereals raw material equal proportion such as beans, mung bean, green soya bean and rde bean is added, and accounts for the 7wt% of the raw material gross weight altogether, and integration of drinking and medicinal herbs is former
Material and five cereals raw material are crushed to 50 mesh, after the mashing of the fruit and vegetable materials such as sugarcane 4wt%, pears 5wt%, lotus root 4wt% and ternip 4wt%, mixing
Uniformly, moisturizing is used in combination citric acid adjusting to make pH=4.0 to 100 parts.
Step 2. digests.To be added acid starch enzyme in solution obtained by step 1, acid pectase, acid protease into
Row complex enzyme hydrolysis, digests 15Min by 35 DEG C.
Step 3. compounds.White granulated sugar is added according to 7% ratio into the enzymolysis liquid obtained by step 2,3% ratio is added
Yeast powder is uniformly mixed, 95 DEG C, and sterilize 20min.
Step 4. after-ripening.Into the combination solution obtained by step 3 lactobacillus plantarum, cheese breast bar are accessed according to 5% ratio
Bacterium, bifidobacterium lactis, Lactobacillus paracasei, acetobacter xylinum, natto withered grass, 25 DEG C, after-ripening 6 months.
Step 5. is added into clear liquid according to 10% ratio through centrifuging or being collected by filtration step 5 acquired solution clear liquid
Functional amino is added in honey, 1% ratio（Its Glutamic Acid 0.5%, alanine 0.2%, leucine 0.15%, lysine
0.15%）, 2% ratio addition hydroxyl isomaltulose etc..
Step 6. is filling and sterilizes.Step 6 acquired solution is filling, 75 DEG C of 15 ~ 30 points of bus sterilization processings are used afterwards
Clock.
Embodiment 2
Step 1. pre-processes.Take pueraria lobata 4wt%, Radix Salviae Miltiorrhizae 4wt%, hawthorn 2wt%, flower of kudzuvine 6wt%, dried orange peel 5wt% and other can relieve the effect of alcohol
Chinese herbal medicine, such as：The integration of drinking and medicinal herbs such as ginseng, Radix Glycyrrhizae and peppermint raw material respectively accounts for the 1wt% and black soya bean, Huang of the raw material gross weight
The five cereals raw material equal proportion such as beans, mung bean, green soya bean and rde bean is added, and accounts for the 7wt% of the raw material gross weight altogether, and integration of drinking and medicinal herbs is former
Material and five cereals raw material are crushed to 60 mesh, after the mashing of the fruit and vegetable materials such as sugarcane 1wt%, pears 4wt%, lotus root 5wt% and ternip 5wt%, mixing
Uniformly, moisturizing is used in combination citric acid adjusting to make pH=5.0 to 100 parts.
Step 2. digests.Acidic cellulase will be added in solution obtained by step 1, acid protease carries out complex enzyme
Solution, 45 DEG C of enzymolysis process digest 30Min.
Step 3. compounds.White granulated sugar is added according to 5% ratio into the enzymolysis liquid obtained by step 2,2% ratio is added
Yeast powder is uniformly mixed, 90 DEG C, and sterilize 15min.
Step 4. after-ripening.Into the combination solution obtained by step 3 streptococcus thermophilus, Bao Jiali are accessed according to 3 ~ 5% ratios
Sub- lactobacillus, lactobacillus plantarum, brewer's wort fermented acetic acid bacterium, speed wine acetic acid bacteria, acetobacter xylinum, Propionibacterium, 20 DEG C, after-ripening 4
A month.
Bee is added according to 5% ratio into clear liquid through centrifuging or being collected by filtration step 5 acquired solution clear liquid in step 5.
Functional amino is added in honey, 1% ratio（Its Glutamic Acid 0.5%, alanine 0.2%, leucine 0.15%, lysine
0.15%）, the functional raw materials such as 3% ratio addition hydroxyl isomaltulose.
Step 6. is filling and sterilizes.Step 6 acquired solution is filling, 80 DEG C of 15 ~ 30 points of bus sterilization processings are used afterwards
Clock.
Embodiment 3
Step 1. pre-processes.Take pueraria lobata 5wt%, Radix Salviae Miltiorrhizae 4wt%, hawthorn 2wt%, flower of kudzuvine 3wt%, dried orange peel 2wt% and other can relieve the effect of alcohol
Chinese herbal medicine, such as：The integration of drinking and medicinal herbs such as ginseng, Radix Glycyrrhizae and peppermint raw material respectively accounts for the 2wt% and black soya bean, Huang of the raw material gross weight
The five cereals raw material equal proportion such as beans, mung bean, green soya bean and rde bean is added, and accounts for the 8wt% of the raw material gross weight altogether, and integration of drinking and medicinal herbs is former
Material and five cereals raw material are crushed to 80 mesh, after the mashing of the fruit and vegetable materials such as sugarcane 5wt%, pears 3wt%, lotus root 5wt% and ternip 4wt%, mixing
Uniformly, moisturizing is used in combination citric acid adjusting to make pH=6.0 to 100 parts.
Step 2. digests.Acid starch enzyme, acid cellulose will be added in solution obtained by step 1 in the solution obtained by step 1
Enzyme, acid protease carry out complex enzyme hydrolysis, and 60 DEG C of enzymolysis process digests 50Min.
Step 3. compounds.White granulated sugar is added according to 10% ratio into the enzymolysis liquid obtained by step 2, ferment is added in 5% ratio
Female powder is uniformly mixed, 100 DEG C, and sterilize 30min.
Step 4. after-ripening.Into the combination solution obtained by step 3 lactobacillus bulgaricus, newborn bifid are accessed according to 3 ~ 5% ratios
Bacillus, Lactobacillus paracasei, Lactobacillus rhamnosus, acetobacter, acetobacter xylinum, Propionibacterium, enterococcus faecalis, natto withered grass,
15 DEG C, after-ripening 4 months.
Honey is added according to 8% ratio into clear liquid through centrifuging or being collected by filtration step 5 acquired solution clear liquid in step 5.,
Functional amino is added in 1% ratio（Its Glutamic Acid 0.5%, alanine 0.2%, leucine 0.15%, lysine 0.15%）,
The functional raw materials such as hydroxyl isomaltulose are added in 2% ratio.
Step 6. is filling and sterilizes.Step 6 acquired solution is filling, it is rear using 80 DEG C of bus sterilization processings 15 ~ 30 minutes.
Certainly, above description is also not limited to the example above, technical characteristic of the present invention without description can by or use
The prior art realizes that details are not described herein；It is not to the present invention that above example, which is only used to illustrate the technical scheme of the present invention,
Limitation, described the invention in detail with reference to preferred embodiment, it will be understood by those of ordinary skill in the art that,
The variations, modifications, additions or substitutions that those skilled in the art are made in the essential scope of the present invention are not
It is detached from spirit of the invention, should also belong to the claims of the present invention.
Claims (9)
1. the present invention relates to a kind of functional relieving alcoholism and protecting liver ferment products, it is characterised in that the ferment is with integration of drinking and medicinal herbs material
Based on material, five cereals, fruits and vegetables, by enzymolysis, composite probiotics ferment and Aging storage, it is multiple to be aided with the auxiliary materials such as functional amino
With into.
2. method as described in claim 1, it is characterised in that the integration of drinking and medicinal herbs raw material mainly include pueraria lobata, Radix Salviae Miltiorrhizae, hawthorn,
One or more of flower of kudzuvine, dried orange peel, ginseng, Radix Glycyrrhizae and peppermint.The five cereals include black soya bean, soya bean, mung bean, green soya bean and red
One or more of red bean.The fruits and vegetables include one or more of sugarcane, pears, lotus root and ternip.
3. method as claimed in claim 2, it is characterised in that above-mentioned integration of drinking and medicinal herbs raw material, five cereals are crushed to 40~100 mesh, fruits and vegetables
After raw material pulping, be mixed uniformly, moisturizing to 100 wt%, be used in combination lemon acid for adjusting pH=4.0~
6.0, all kinds of raw materials number by weight is as follows：
10 ~ 15wt% of pueraria lobata
7 ~ 26wt% of other integration of drinking and medicinal herbs raw materials
5 ~ 10wt% of five cereals
6 ~ 23wt% of fruits and vegetables.
4. method as described in claim 1, it is characterised in that complex enzyme hydrolysis technique selects two kinds below according to 1 ~ 3 ‰ ratio
Or two or more enzyme preparations, 30~60 DEG C, digest 10~60Min.The enzyme includes mainly：Acid starch enzyme, acid pectin
Enzyme, acidic cellulase, acid protease.
5. method as described in claim 1, it is characterised in that the composite probiotics ferment stage first into enzymolysis liquid according to 5~
White granulated sugar is added in 10% ratio, and yeast powder is added in 2~5% ratio, after mixing 90~100 DEG C of 15~30min of sterilizing, so
Afterwards compound probiotic is accessed according to 3~5% ratios.
6. method as claimed in claim 5, it is characterised in that compound probiotic includes following three kinds or more：Thermophilus
Bacterium, lactobacillus bulgaricus, lactobacillus plantarum, Lactobacillus casei, bifidobacterium lactis, Lactobacillus paracasei, Lactobacillus rhamnosus,
Acetobacter, brewer's wort fermented acetic acid bacterium, speed make acetic acid bacteria, acetobacter xylinum, Propionibacterium, enterococcus faecalis, natto withered grass.
7. method as described in claim 1, it is characterised in that after-ripening condition is：15~25 DEG C, after-ripening 3~6 months.
8. method as described in claim 1, it is characterised in that after-ripening liquid through centrifuging or being collected by filtration clear liquid, and into clear liquid according to
Honey is added in 5~10% ratio, and functional amino is added in 0.5~1% ratio, and hydroxyl isomaltulose is added in 2~3% ratio
Equal functional raw materials, after product is filling after compounding using 75~80 DEG C of bus sterilizations at
Reason 15~30 minutes.
9. method as claimed in claim 8, it is characterised in that functional amino includes：Glutamine, alanine, leucine,
Lysine, adding proportion 1:2:1:1.
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| CN110679934A (en) * | 2019-10-28 | 2020-01-14 | 青岛佳一生物科技股份有限公司 | Lutein-containing enzyme capable of clearing liver and improving eyesight and preparation method thereof |
| CN110946278A (en) * | 2019-12-03 | 2020-04-03 | 陕西慧创农业科技有限公司 | Preparation method of hovenia dulcis compound enzyme |
| CN111109482A (en) * | 2019-12-24 | 2020-05-08 | 武汉轻工大学 | Composite enzymatic silybum marianum enzyme and preparation method and application thereof |
| CN115191532A (en) * | 2022-06-16 | 2022-10-18 | 武汉新华扬生物股份有限公司 | Hangover-alleviating enzyme and preparation method thereof |
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