CN108714214A - A kind of pure natural gynaecology Chinese juniper lotion and preparation method thereof - Google Patents
A kind of pure natural gynaecology Chinese juniper lotion and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Abstract
The present invention relates to a kind of pure natural gynaecology Chinese juniper lotions and preparation method thereof, the pure natural gynaecology Chinese juniper lotion includes Chinese juniper extract 45-76 weight %, protease 18-23 weight %, yolk antibody IgY 0.15-0.8 weight %, rice bran extract 0.15-0.9 weight % and surplus purified water.Pure natural gynaecology Chinese juniper lotion of the present invention can effectively inhibit the growth of Candida albicans, staphylococcus aureus, hemolytic streptococcus, and safeguard that vagina normal flora is grown, Inflammatory effects are significantly improved to vaginitis, cervicitis and suppurative genitals inflammation.
Description
Technical field
The present invention relates to disinfecting articles for use, especially a kind of pure natural gynaecology Chinese juniper lotion and preparation method thereof.
Background technology
Gynecological disease is women common disease, and incidence is higher, and is easy repeatedly.Gynecological disease majority is derived from vagina infection,
Due to lacking the health care to body, various bad life habits, vagina infection exacerbation in addition cannot but be inhibited in time, in turn
Develop into uterine disease, diseases of fallopian tubes, disease of ovary etc..
Vaginal lotion currently on the market use chemical formulation, simulate vagina environment, usually contain phenylate, metronidazole,
The growth of interference vagina normal flora is used for a long time in Chlorhexidine etc., causes a variety of abdominal pain and inflammation repeatedly etc..It adopts in the prior art
Relatively mild lotion is obtained with Chinese medicine preparation, however the extraction of middle the effective elements of the medicine is difficult, it is difficult to mass production, and using effect
Upper slowly effect, market groups are poor to the informativeness of product.Patent application CN105456644A discloses one kind and is split for perineum
The external-use lotion and preparation method thereof for hindering postoperative care, using sand seven, soulie barberry bark, shortleaf peashrub, watter milkwort root leaf or bark, yunnan machilus leaf, powder
Tumor bacterium, fragile blueberry root, tiger ear, the prince's descendants, Sievers wormwood, Bai Gen, Lysimachia christinae, dunn antiotrema root, Gotu Kola P.E, Gentrin Knotweed P.E are matched
Chinese medicine is made, with polyethylene glycol 400, chlorhexidine acetate, glycerine is mixed and made into external-use lotion, is needed in production by numerous Chinese medicine
Ingredient drying crushes, the extraction of ultrasonic ethyl alcohol, and technological process is long, and production cost is high, and contains chlorhexidine acetate, toxicity be it is medium,
It is unfavorable for being used for a long time.
Invention content
Problem to be solved by this invention is to overcome the shortcomings of the prior art, provides a kind of pure natural gynaecology Chinese juniper lotion
And preparation method thereof, toxic chemical is not contained, using pure natural formula
Applicant in order to improve gynecological lotion currently on the market contain different degrees of low toxicity, be poisoned substance threadiness,
It explores under the growth conditions for not influencing the normal strain of intravaginal, improves vagina environment.Generally use traditional Chinese medicine ingredients can reach
To certain effect, however the slowly effect of herbal treatment, the expection of the consumer group is cannot be satisfied, even if improving containing for traditional Chinese medicine ingredients
Amount, still can not solve root problem.By constantly groping, developed finally with Chinese juniper extract pure natural woman as main component
Section's Chinese juniper lotion.
In the present invention, Chinese juniper extract is the water extract of Chinese juniper wood, containing Chinese juniper wood polyphenol powde, not only has aromatic odor, energy
Package odor molecules are quickly neutralized, and have clean effect, and have effects that anti-inflammation and sterilization.
Chinese juniper extract acidity is too strong, directly applies to vaginadouche and needs to dilute, and excess dilution can cause substantive effect
The decline of fruit, for this purpose, it is that the invention solves another major issues to improve the pH of formula using pure natural Hubeiwan.
Rice bran extract mainly contains ceramide in the present invention, has protection vagina moisture holding capacity, maintains skin elasticity,
And there is antiallergic effect.Particularly, rice bran extract alkalinity is stronger, can reach satisfied pH ranges with the cooperation of Chinese juniper extract, full
The requirement of sufficient gynecological lotion.And corresponding antibacterial experiment effect protrudes.
In the present invention, fast decoupled human secretion is reached by protease.The effect of yolk antibody IgY be antibacterial and
Do not influence normal flora.Commercial product may be used in yolk antibody IgY, the chicken produced such as the Hangzhou bio tech ltd Ya Sheng
Yolk immunoglobulin can also be made by oneself, the method that shown below antibody purification.Antibody (IgY) is extracted from yolk as follows：
First, TBS (20mM Tris-HCl, the 0.15M NaCl and 0.5%NaN of 5 times of yolk amounts are added into yolk3) and equivalent
10% dextran sulfate/TBS, and stir the mixture for 20 minutes.In addition, the 1M CaCl with yolk equivalent are added2/
TBS is stirred the mixture for and is incubated 12 hours.Hereafter, mixture is centrifuged 20 minutes at 15000rpm, collects supernatant.It connects
Get off, ammonium sulfate is added and is incubated 12 hours at 4 DEG C to final concentration of 40%, and by mixture.Later, mixture is existed
It is centrifuged 20 minutes under 15000rpm, collects precipitation.Finally, precipitation is resuspended in the TBS of yolk equivalent, TBS is used in combination to dialyse.
The IgY that purity is 90% or more can be collected by this technique.The IgY of 2-4g can be purified in one yolk.
Concrete scheme is as follows：
A kind of pure natural gynaecology Chinese juniper lotion, including following components：
Wherein, the Chinese juniper extract is the water extract of Chinese juniper wood.
Further, the pure natural gynaecology Chinese juniper lotion is composed of the following components：
Further, the pure natural gynaecology Chinese juniper lotion is composed of the following components：
Further, the Chinese juniper extract is water extract of the Chinese juniper wood at 80-95 DEG C.
Further, the protease is acid protease.
Further, the yolk antibody IgY is the yolk antibody IgY extracted in egg, and purity is more than or equal to 90%.
The present invention also protects the preparation method of the pure natural gynaecology Chinese juniper lotion, includes the following steps：
1) fresh Chinese juniper wood is taken, is added in purified water after broken and carries out distillation extraction, collects fraction, it is cooling to obtain Chinese juniper extraction
Object；
2) rice bran extract is added into Chinese juniper extract, makes pH=4.2-4.6, obtain solution A；
3) protease, yolk antibody IgY are added to purified water under conditions of no more than -20 DEG C, are uniformly mixed, obtain
Solution B；
4) solution A and solution B are mixed, sterilizing is sealed up for safekeeping to get the pure natural gynaecology Chinese juniper lotion.
Further, the temperature that extraction is distilled described in step 1) is 80-95 DEG C.
Further, the mode being uniformly mixed in step 3) is sonic oscillation.
The present invention also protects the pure natural gynaecology that the preparation method with the pure natural gynaecology Chinese juniper lotion is prepared
Chinese juniper lotion.
Advantageous effect：Pure natural gynaecology Chinese juniper lotion of the present invention can effectively inhibit Candida albicans, golden yellow grape
The growth of coccus, hemolytic streptococcus, and safeguard that vagina normal flora is grown, to vaginitis, cervicitis and suppurative genitals
Inflammation is significantly improved Inflammatory effects.
Specific implementation mode
Technical solution of the present invention is further elaborated with reference to embodiment.Particular technique or item are not specified in embodiment
Part person carries out according to technology or condition described in document in the art or according to product description.Agents useful for same or instrument
Production firm person is not specified in device, and being can be with conventional products that are commercially available.
Embodiment 1
Pure natural gynaecology Chinese juniper lotion is prepared, method is as follows：
1) fresh Chinese juniper wood is taken, is added in purified water after broken and carries out distillation extraction, be heated to 90 DEG C, collects fraction, it is cold
But Chinese juniper extract is obtained；
2) rice bran extract is added into Chinese juniper extract, makes pH=4.2, obtain solution A；
3) acid protease, yolk antibody IgY are added to purified water under conditions of no more than -20 DEG C, are uniformly mixed,
Obtain solution B；
4) solution A and solution B are mixed, sterilizing is sealed up for safekeeping to get the pure natural gynaecology Chinese juniper lotion.
Each component content is shown in Table 1.In preparation process, the temperature of Chinese juniper wood distillation extraction should be no more than 95 DEG C, to avoid excessive
Oil components steam, and cause separation difficult；Acid protease, yolk antibody IgY must mix under the conditions of no more than -20 DEG C,
To reduce enzymatic activity.Rice bran extract alkalinity is higher, it is necessary to Chinese juniper extract neutralize after again with acid protease, Yolk antibody
IgY is mixed, and otherwise can influence the using effect of protease and antibody.
Table 1 prepares pure natural gynaecology Chinese juniper lotion scale (parts by weight)
| Component | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| Chinese juniper extract | 68 | 62 | 70 | 55 | 90 |
| Protease | 7 | 12 | 14 | 15 | 5 |
| Yolk antibody IgY | 0.6 | 0.3 | 0.15 | 0.7 | 0.8 |
| Rice bran extract | 0.4 | 0.7 | 0.15 | 0.9 | 0.2 |
| Purified water | 24 | 25 | 15.7 | 28.4 | 4 |
Pure natural gynaecology Chinese juniper lotion prepared by the present invention belongs to natural antibacterial washing lotion, can effective protection beneficial flora.It goes
Dirty ability is strong, and can eliminate the unusual smell moment, improves pruritis, is verified by external germ experiment, to Candida albicans, golden yellow
Color staphylococcus, hemolytic streptococcus bacteriostasis rate respectively reach 100%, 99.97% and 99.99%.
Embodiment 2
Pure natural gynaecology Chinese juniper lotion is prepared, method is as follows：
1) fresh Chinese juniper wood is taken, is added in purified water after broken and carries out distillation extraction, is heated to 80 DEG C of collection fractions, it is cooling
Obtain Chinese juniper extract；
2) rice bran extract is added into Chinese juniper extract, makes pH=4.6, obtain solution A；
3) acid protease, yolk antibody IgY are added to purified water under conditions of no more than -20 DEG C, sonic oscillation
It is uniformly mixed, obtains solution B；
4) solution A and solution B are mixed, sterilizing is sealed up for safekeeping to get the pure natural gynaecology Chinese juniper lotion.
Each component content is shown in Table 1, external germ experiment verification, to Candida albicans, staphylococcus aureus, hemolytic
Streptococcic bacteriostasis rate respectively reaches 100%, 99.96% and 99.99%.
Embodiment 3
Pure natural gynaecology Chinese juniper lotion is prepared, method is as follows：
1) fresh Chinese juniper wood is taken, is added in purified water after broken and carries out distillation extraction, be heated to 95 DEG C, collects fraction, it is cold
But Chinese juniper extract is obtained；
2) rice bran extract is added into Chinese juniper extract, makes pH=4.4, obtain solution A；
3) acid protease, yolk antibody IgY are added to purified water under conditions of no more than -20 DEG C, sonic oscillation
It is uniformly mixed, obtains solution B；
4) solution A and solution B are mixed, sterilizing is sealed up for safekeeping to get the pure natural gynaecology Chinese juniper lotion.
Each component content is shown in Table 1, external germ experiment verification, to Candida albicans, staphylococcus aureus, hemolytic
Streptococcic bacteriostasis rate respectively reaches 100%, 99.97% and 99.98%.
Embodiment 4
Pure natural gynaecology Chinese juniper lotion is prepared, method is as follows：
1) fresh Chinese juniper wood is taken, is added in purified water after broken and carries out distillation extraction, is heated to 85 DEG C of collection fractions, it is cooling
Obtain Chinese juniper extract；
2) rice bran extract is added into Chinese juniper extract, makes pH=4.5, obtain solution A；
3) acid protease, yolk antibody IgY are added to purified water under conditions of no more than -20 DEG C, are uniformly mixed,
Obtain solution B；
4) solution A and solution B are mixed, sterilizing is sealed up for safekeeping to get the pure natural gynaecology Chinese juniper lotion.
Each component content is shown in Table 1, is used for clinical trial using pure natural gynaecology Chinese juniper lotion manufactured in the present embodiment, acquisition is cloudy
Road inflammation, cervicitis and suppurative genitals inflammation patient case 20, randomly select 15 uses pure day manufactured in the present embodiment
Right gynaecology Chinese juniper lotion, daily 1-2 progress private parts flushing, in addition 5 can pay a return visit for one month as a contrast, case 14 of fully recovering
(ill symptoms disappearance clinically occur), (clinically there is ill symptoms mitigation) in effective case 1, and control group changes without any
It is kind.Record is paid a return visit to show that vagina environment can be significantly improved within one week or so using pure natural gynaecology Chinese juniper lotion prepared by embodiment,
There is no adverse reaction during follow-up use, leukorrhea inspection is carried out to 14 people of experimental group recovery from illness after one month, cleanliness factor is all
For I degree, vagina microorganism is all negative, illustrates that vagina environment is restored to normal level.
Embodiment 5
Pure natural gynaecology Chinese juniper lotion is prepared, method is as follows：
1) fresh Chinese juniper wood is taken, is added in purified water after broken and carries out distillation extraction, is heated to 95 DEG C of collection fractions, it is cooling
Obtain Chinese juniper extract；
2) rice bran extract is added into Chinese juniper extract, makes pH=4.6, obtain solution A；
3) protease, yolk antibody IgY are added to purified water under conditions of no more than -20 DEG C, are uniformly mixed, obtain
Solution B；
4) solution A and solution B are mixed, sterilizing is sealed up for safekeeping to get the pure natural gynaecology Chinese juniper lotion.
Each component content is shown in Table 1, external germ experiment verification, to Candida albicans, staphylococcus aureus, hemolytic
Streptococcic bacteriostasis rate respectively reaches 100%, 99.97% and 99.98%.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art are not departing from the principle of the present invention and objective
In the case of can make changes, modifications, alterations, and variations to the above described embodiments within the scope of the invention.
Claims (10)
1. a kind of pure natural gynaecology Chinese juniper lotion, it is characterised in that：Including following components：
Wherein, the Chinese juniper extract is the water extract of Chinese juniper wood.
2. pure natural gynaecology Chinese juniper lotion according to claim 1, it is characterised in that：It is composed of the following components：
3. pure natural gynaecology Chinese juniper lotion according to claim 1, it is characterised in that：It is composed of the following components：
4. pure natural gynaecology Chinese juniper lotion according to claim 1, it is characterised in that：The Chinese juniper extract is Chinese juniper wood in 80-
95 DEG C of water extract.
5. pure natural gynaecology Chinese juniper lotion according to claim 1, it is characterised in that：The protease is acid protease.
6. pure natural gynaecology Chinese juniper lotion according to claim 1, it is characterised in that：The yolk antibody IgY is in egg
The yolk antibody IgY of extraction, purity are more than or equal to 90%.
7. the preparation method of pure natural gynaecology Chinese juniper lotion described in claim 1, it is characterised in that：Include the following steps：
1) fresh Chinese juniper wood is taken, is added in purified water after broken and carries out distillation extraction, collects fraction, it is cooling to obtain Chinese juniper extract；
2) rice bran extract is added into Chinese juniper extract, makes pH=4.2-4.6, obtain solution A；
3) protease, yolk antibody IgY are added to purified water under conditions of no more than -20 DEG C, are uniformly mixed, obtain solution
B；
4) solution A and solution B are mixed, sterilizing is sealed up for safekeeping to get the pure natural gynaecology Chinese juniper lotion.
8. the preparation method of pure natural gynaecology Chinese juniper lotion according to claim 7, it is characterised in that：It is steamed described in step 1)
The temperature for evaporating extraction is 80-95 DEG C.
9. the preparation method of pure natural gynaecology Chinese juniper lotion according to claim 7, it is characterised in that：Mixing is equal in step 3)
Even mode is sonic oscillation.
10. being prepared with the preparation method of the pure natural gynaecology Chinese juniper lotion of claim 7-9 any one of them pure natural
Gynaecology's Chinese juniper lotion.
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| 郭雪飞: "与医药保健品有关的几种林产化学品研究现状", 《生物质化学工程》 * |
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