CN108686264A - 离子液体溶解骨骼的一种方法工艺 - Google Patents

离子液体溶解骨骼的一种方法工艺 Download PDF

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CN108686264A
CN108686264A CN201710230689.0A CN201710230689A CN108686264A CN 108686264 A CN108686264 A CN 108686264A CN 201710230689 A CN201710230689 A CN 201710230689A CN 108686264 A CN108686264 A CN 108686264A
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bone
dissolving
ion liquid
ionic liquid
liquid
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邓宇
阮孝慈
李佳彤
杨洋
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Tianjin University of Science and Technology
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Tianjin University of Science and Technology
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/02—Inorganic materials
    • A61L27/12—Phosphorus-containing materials, e.g. apatite
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3608—Bone, e.g. demineralised bone matrix [DBM], bone powder
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Orthopedic Medicine & Surgery (AREA)
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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Abstract

离子液体溶解骨骼的一种方法工艺，以鸡骨为原料，选择氯化胆碱‑甘油低共融体系离子液体，采用研磨法对骨骼进行溶解，设计单因素、正交实验确定离子液体中骨骼的最佳溶解工艺。其工艺条件为：鸡骨与氯化胆碱‑甘油质量比(固液比)为1∶20，反应溶解时间4h，溶解温度115℃。离子液体回收率为95.17％，所得溶解产物为胶原与羟基磷灰石。

Description

离子液体溶解骨骼的一种方法工艺
技术领域 本发明涉及一种溶解天然骨的新型方法与工艺，属于精细化学中天然物提取制备新型材料的技术领域。
背景技术 据统计我国每年产生的动物骨骼量高达1500万吨，其中有100万吨未在骨骼加工领域得到有效利用，造成了资源浪费。骨骼主要是由有机成分胶原和无机成分羟基磷灰石组成。其中，骨骼中胶原主要用于生物医学、食品加工、化妆品制备等领域；骨骼中羟基磷灰石具有良好的生物相容性和组织活性，被广泛用做骨损伤修复材料。一方面，离子液体具有无污染、较低蒸汽压、不易燃、易与产物分离、可设计性、易回收、可循环使用等优点，选用新型绿色溶剂溶解骨骼符合可持续发展技术要求，具有经济效益；另一方面，动物骨骼中含有丰富的骨胶原和相容性良好的生物材料羟基磷灰石，探究离子液体溶解的工艺条件，提取有机成分骨胶原，并分离出无机成分羟基磷灰石，为新型生物材料的制备提供了研究方法，避免了资源浪费。
发明内容 本发明的目的是提供一种新的溶解骨骼的方法及其溶解工艺条件，其设备简单，原料易得又经济节能。本发明利用氯化胆碱-甘油离子液体溶解鸡骨，确定最佳溶解工艺，提取制备生物材料。离子液体溶解骨骼的原理是根据氢键破坏理论，利用离子液体破坏骨骼中胶原的三股螺旋结构、胶原多肽之间的肽键、胶原与羟基磷灰石分子间氢键，从而溶解骨骼中有机成分胶原，提取分离出羟基磷灰石，达到溶解骨骼的目的。与常规制备羟基磷灰石的方法相比，此工艺设备简单，原料易得，变废为宝，成本较低，采用生物提取法所得产物相容性更好，方法更为新颖。其特征在于所述的研磨法溶解骨骼过程界面更新更为均匀，所述原料鸡骨的粒度为60～80目；所述离子液体的合成原料氯化胆碱与丙三醇摩尔比为1∶2；所述离子液体溶解鸡骨的反应时，鸡骨与离子液体的质量比(固液比)可为1∶10～1∶50；溶解时间可为2h～6h；溶解温度可为80℃～160℃。
具体实施方式 称取1g鸡骨(60目～80目)置于盛有15g离子液体的研钵中，在加热装置中加热研磨搅拌。在115℃下溶解；4h后结束反应，趁热迅速用铜网过滤，去除未反应鸡骨颗粒，将滤渣洗净后干燥称重，得到鸡骨在离子液体中的溶解率为47.3％；待离子液体温度降至室温时加入甲醇进行稀释，充分混合后迅速进行真空抽滤，得到提取产物羟基磷灰石，蒸馏水少量多次洗涤，干燥后称重，提取率为22.3％；滤液用旋转蒸发仪旋蒸回收再利用，回收次数为3次，平均回收率为95.17％。

Claims (4)

1.一种溶解天然骨的新型方法与工艺，其特征在于所述的研磨法溶解骨骼过程界面更新更为均匀，选用离子液体溶解骨骼中胶原，从而提取分离出骨骼中羟基磷灰石，并应用于骨损伤修复材料。选用的原料鸡骨粒度为60～80目。
2.按权利要求1所述的离子液体溶解骨骼的方法工艺，所述离子液体的合成原料氯化胆碱与丙三醇摩尔比为1∶2。
3.按权利要求1所述离子液体溶解骨骼的方法时，鸡骨与离子液体的质量比(固液比)可为1∶10～1∶50；溶解时间可为2h～6h；溶解温度可为80℃～160℃。
4.按权利要求3所述离子液体溶解骨骼的工艺，溶骨率可达47.3％，羟基磷灰石提取率可达22.3％。
CN201710230689.0A 2017-04-07 2017-04-07 离子液体溶解骨骼的一种方法工艺 Pending CN108686264A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115990289A (zh) * 2023-01-09 2023-04-21 西岭(镇江)医疗科技有限公司 一种不完全脱钙骨诱导材料的制备方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115990289A (zh) * 2023-01-09 2023-04-21 西岭(镇江)医疗科技有限公司 一种不完全脱钙骨诱导材料的制备方法
CN115990289B (zh) * 2023-01-09 2024-05-31 西岭(镇江)医疗科技有限公司 一种不完全脱钙骨诱导材料的制备方法

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