CN108684669A - A kind of composition pesticide containing trans-ferulaic acid and Physcion and its application - Google Patents
A kind of composition pesticide containing trans-ferulaic acid and Physcion and its application Download PDFInfo
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- CN108684669A CN108684669A CN201810646059.6A CN201810646059A CN108684669A CN 108684669 A CN108684669 A CN 108684669A CN 201810646059 A CN201810646059 A CN 201810646059A CN 108684669 A CN108684669 A CN 108684669A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
Abstract
The present invention relates to a kind of composition pesticide containing trans-ferulaic acid and Physcion and its applications, the composition pesticide, and active constituent includes trans-ferulaic acid and Physcion.The present invention is compounded by the pesticide of different role spectrum and different role mechanism, on the one hand expand action spectrum, on the other hand it can fast and simply carry out and delay disease drug resistance approach, more importantly meets the needs of market rapidly in the field of crucial shortage simultaneously, paces are accelerated to substitute pesticide update, ensure the safety of grain and vegetables.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of pesticide group containing trans-ferulaic acid and Physcion
Close object and its application.
Background technology
The prevention of virosis no matter be all for animal or plant research difficult point, the mechanism of action, variation soon with
And route of transmission the problem of being all researcher headache, and for plant virosis be even more have " plant cancer " it
Claim, plant ecological pesticide is used to prevent the development of viroses of plant etc. and develops gradually by the height of agricultural chemical R&D
Concern, becomes the hot spot of agriculture and forestry sustainable development, is the effective way for improving environment, reduction or delaying drug resistance and improving drug effect
Diameter.Wherein, disease-resistant, immune one of the hot spot for having become biological pesticide research of activation, the final decrement for realizing pesticide is induced to use.
The quality and quantity of the prevention plant virus medicament of existing market exploitation far can not meet the needs of agricultural production.
The plant virus being currently known has 716 kinds (being turned into good etc., plant virus taxology, Chinese agriculture publishing house, 1996,283),
Some documents are thought at 1000 kinds or more.The research topic of the prevention viroses of plant shoulders heavy responsibilities.
Plant virus endangers the various plants such as unifacial leaf, dicotyledonous and microorganism, and distribution is very universal, and common cultivation is planted
Each crop of object has a kind of to a variety of virus harm, harm to be only second to fungal disease.Virus after infecting host not only with post
Nutritional ingredient necessary to main contention plant growth, and the nutrient conduction of plant is destroyed, change certain metabolism of host plant
The activity of balance and some enzymes causes plant growth difficult, generates the symptoms such as deformity, yellow, serious to also cause host plant
Death, lead to crop failure and quality decline.Its harm is extremely serious, and such as yellow stunt of wheat, nineteen fifty-one is for the first time in U.S.
State find, become worldwide disease, start the beginning of the sixties Shaanxi harm, 1960,1964,1966,1970,1973,
1978, it is endangered in northern China area of wheat prevalence within 1980,1999, the average year underproduction 30% reaches individually 60%.
Endangering virosis outstanding in China mainly has:Yellow stunt of wheat, yellow stunt of rice, wheat yellowtop, potato
Degeneration, tomato, rape and Chinese cabbage mosaic disease, wheat rosette stunt, corn striped mosaic disease, millet red-leaf disease, rice secret note
It is dwarf wilt, tobacco mosaic disease, yellow virosis of beets disease, soybean mosaic, apple rough skin disease, apple mosaic, citrus yellow shoot disease, melon
Mosaic disease and peanut withes broom etc..Plant disease seriously hinders further increasing for level of agricultural production, how to control, mitigates
Or the harm of killing off plant virus has become the problem of whole mankind pays close attention to.Chemical prevention plant virus have it is quick,
It is easy to use, agriculture sheet is low, easy to use and to inhibit virus spectrum wide etc. many a little.Largely it turns out that plant virus chemistry is anti-
It controls agent and is occupied an important position in the prevention of plant disease as fungicide.
The prevention spectrum of plant virus chemical prevention agent is quite extensive, however from actual production, chemical prevention agent is to planting
The control effect of object virus is usually between 30~70%, and most of is the biological complex agent type with chemical classes.It is led
It is that inhibitors of viral replication, deactivation matter, defense response exciton and physiologically active of plant Auto-regulator etc. are a kind of mixed to want ingredient
With object.Inhibitors of viral replication mainly uses Moroxydine Hydrochloride, virazole, Ningnanmycin and amino-oligosaccharide etc..
It is imprecise in current many chemopreventive effects, lack special effective scheme, is cured the disease come anti-using theory of traditional Chinese medical science
Viral disease occupies apparent advantage, this has obtained sufficient confirmation in the preventing and controlling of SARS virus, and many documents are also this
Sample is discussed.Therefore, it in the prevention of the viroses of plant etc., is immunized using the activation of theory of traditional Chinese medical science zygotic induction and is planted to inquire into biological control
Object virosis, and then prevent a variety of fungies, bacteriosis with important research significance.
Invention content
The purpose of the present invention is compounded by the pesticide of different role spectrum and different role mechanism, it is intended to crucial short slab
The prevention and control fields such as the viroses of plant meet the needs of market rapidly, moreover it is possible to expand prevention spectrum, furthermore again can it is quick and simply
Implementation delays disease drug resistance, in the today for advocating biological control, reduces the use of chemical pesticide, improves environment, reduces or prolong
Slow resistance improves drug effect, provides a kind of composition pesticide containing trans-ferulaic acid and Physcion and its application.
Technical solution of the present invention is as follows:
A kind of composition pesticide, active constituent include trans-ferulaic acid and Physcion.
Further, the mass ratio of its active constituent trans-ferulaic acid and Physcion is 1 in the composition pesticide:
900~900:1, preferably 50:1~1:40;Further preferably 55:1~50:10;More preferably 50:1~50:5.
In the specific embodiment of the invention, its active constituent trans-ferulaic acid and rheum emodin first in the composition pesticide
The mass ratio of ether is 50:1 or 50:5.
It is demonstrated experimentally that trans-ferulaic acid can both expand to plant virus controlling with Physcion within the scope of said ratio
Action spectrum, moreover it is possible to synergistic function is played to the effect of plant virus controlling, conducive to disease drug resistance is delayed.
The present invention also provides a kind of pesticides, can be separately made by above-mentioned composition pesticide, also can be by above-mentioned composition pesticide
It is made with the available carrier of pesticide field or auxiliary agent.
Further, the content of the quality sum of trans-ferulaic acid and Physcion is 0.1-90% in the pesticide
(referring to mass fraction, similarly hereinafter), preferably 4-6%, further preferably 5-5.5%.
In the specific embodiment of the invention, in the composition pesticide quality of trans-ferulaic acid and Physcion it
The content of sum is 5.1%;The content of wherein preferred trans-ferulaic acid and Physcion is respectively 5% and 0.1%.
In the specific embodiment of the invention, in the composition pesticide quality of trans-ferulaic acid and Physcion it
The content of sum is 5.5%;The content of wherein preferred trans-ferulaic acid and Physcion is respectively 5% and 0.5%.
The above-mentioned pesticide of the present invention is mainly used as prevention plant virus.
Respective carrier or auxiliary agent, system can be added according to method known in those skilled in the art in the above-mentioned pesticide of the present invention
Agriculturally may be used at suspending agent, water dispersible granules, missible oil, microemulsion, aqueous emulsion, soluble liquid, wettable powder, granule etc.
The dosage form of receiving.Such as available carrier or auxiliary agent include solvent, emulsifier, wetting agent, stabilizer, antifreezing agent, antifoaming agent, increasing
Thick dose or it is other be beneficial to active ingredient in the formulation stablize and drug effect play known substance, be all in field of pesticide preparation often
With or allow the various composition used.
Specifically, the pesticide is suspending agent, and each component grain size is less than or equal to 5 microns.
Composition pesticide of the present invention and the pesticide can be prepared by this field conventional method.
The invention also includes the application of the composition pesticide or the pesticide on plant virus controlling.
Further, controlling object include tobacco, tomato, cucumber, capsicum, apple, rice, corn, wheat, potato,
The vegetable melon and fruits such as clover and crops etc..
Further, the plant virus includes tobacco (floral leaf) virus, tomato virus, cucumber virus, capsicum apple disease
Poison, Rice Virus, corn virus, Wheat Virus, potato (Y) virus, clover virus etc..
The raw materials used in the present invention is commercially available to buy, or is prepared by this field conventional method.
On the basis of common knowledge of the art, above-mentioned each optimum condition can be combined with each other each preferably to get the present invention
Example.
Furthermore, it is to be understood that herein, also included in the meaning of terms such as "include", "comprise", " containing " " by ...
Composition ", " by ... constitute ", " by ... it is made " etc..
Physcion is the active constituent extracted from polygonaceae plant giant knotweed, rheum officinale, special to the preventive effect of powdery mildew
Not significantly, while also there is certain effect to gray mold, anthracnose and virosis.Physcion is by inducing crop to generate
Blade specific thionine albumen with antibacterial activity inhibits the formation of pathogen appresorium and haustorium, so that crop is exempted from
It is encroached on by pathogen, achievees the effect that diseases prevention.
Ferulic acid has the antibacterial action of wide spectrum, can inhibit the growth of Gram-positive, negative bacterium and yeast and numerous
It grows, can inhibit to be caused a disease by bacillus ceylonensis A, pneumobacillus, enterobacteria, Escherichia coli, citric acid bacillus, Pseudomonas aeruginosa etc.
Property bacterium caused by corruption.Ferulic acid has stronger inhibitory activity to fragrant amino N-acetyl-transferase in bacterium, this may be
It inhibits bacterial growth Zhu Yaoyuanyin [Rebecca J.Robbins.Phenolic acids in foods:An
Overview of analytical methodology.J.Agri.Food Chem., 2003,51 (10):2866–2887.].
Trans-ferulaic acid is to extract to obtain from the plant materials such as rice bran, husk and asafoetide, can also be by chemistry side
Method obtains, and structure is completely the same.Trans-ferulaic acid is inhibited to some viruses and bacterium.Suppression of the ferulic acid to virus
Mechanism processed may with inhibit the conversion of AP-1 it is related, AP-1 be inhibit inflammation, cell differentiation, cell Proliferation Huo Xingdanbai [Ou
SY, Kwork K.Review ferulic acid:Pharmaceutical functions, preparation and
Applications in foods.J.Sci.Food Agric., 2004,84:1261–1269.].Ferulic acid can significantly press down
The growth and breeding of influenza virus, Respiratory Syncytial Virus(RSV), AIDS processed.
The present invention is compounded by the pesticide of different role spectrum and different role mechanism, on the one hand expands action spectrum, separately
On the one hand it can fast and simply carry out and delay disease drug resistance approach, while is more importantly rapid in the field of crucial shortage
Meet the needs of market, accelerates paces to substitute pesticide update, ensure the safety of grain and vegetables.
The present invention utilizes theory of traditional Chinese medical science, directly inhibits the duplication and transcription of virus by ferulic acid, is aided with Physcion
Induction, the immune function of activation, the two complements each other from the mechanism of action, can more perfectly realize biological control virosis
Ideal effect can reach the double effects of prevention and treatment, and then a variety of fungies of simultaneous prevention, bacteriosis, be currently on the market
The favourable agents for the controlling disease seldom seen.Meanwhile the present invention can also reduce the use of active ingredient dosage, reduce cost,
The generation for fighting pharmacological property has retarding action, while having ensured the safety and product of vegetables and Tea Production process to a certain extent
Matter.
Compared with prior art, the present invention has following beneficial technique effect:
(1) the two complements each other from the mechanism of action, can more perfectly realize the ideal effect of biological control virosis, energy
Reach the double effects of prevention and treatment.It is the favourable agents for the controlling disease seldom seen currently on the market.
(2) Physcion is different with the mechanism of action of ferulic acid, in prevention and control fields such as the viroses of plant of crucial short slab
Can meet the needs of market rapidly, expand prevention spectrum.
(3) trans-ferulaic acid and Physcion can reduce the amount of application of each single dose after mixture by a certain percentage, improve anti-
Control effect.
Specific implementation mode
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..It is not specified in embodiment specific
Technology or condition person carry out according to technology or condition described in document in the art, or according to product description.It is used
Production firm person is not specified in reagent or instrument, is the conventional products that can be commercially available by regular distributor.
Physcion original powder content range 5~99% used below originates from Jiangsu Huai'an Wei Wei plants research institute;It
So extraction trans-ferulic content:99%, originate from Zhejiang Delekang Food Co., Ltd.;Remaining reagent is commercially available production
Product.
Surfactant:NNO (naphthalene sulfonate), sodium lignin sulfonate, polycarboxylate, ABSNa (dodecyl benzene sulfonic acid
Sodium), MNS/90 (lignosulfonates) etc..
Other auxiliary materials:Antifoaming agent (silicon ether copolymer), wetting agent (HRLD series), ammonium sulfate, cornstarch, fine particle calcium carbonate etc..
Following materials are intended to reach dehydration constant weight.
Embodiment 1 5.1% trans-ferulaic acid Physcion suspending agent (+0.1% rheum emodin first of 5% trans-ferulaic acid
Ether) preparation
Formula:The original powder 1% (wherein Physcion mass content is 10%) of Physcion, trans-ferulaic acid original powder
5.05% (wherein ferulaic acid content is 99%), sodium lignin sulfonate 3%, glycerine 5%, xanthans 0.2%, aluminium-magnesium silicate
0.4%, potassium sorbate 0.5%, antifoaming agent 0.3%, water complements to 100%.
Preparation method:
1) suitable water is weighed, formula is then pressed and sodium lignin sulfonate is added, it is former that Physcion fully is added after dissolving
Powder, is then added glycerine, potassium sorbate, aluminium-magnesium silicate, antifoaming agent 0.3%, is sufficiently mixed uniformly.
2) xanthans is slowly added to while stirring in suitable quantity of water, is made into xanthans mother liquor, step 1) is then added and is made
Mixed material in, make xanthans reach formula ratio;Water is supplied again to formula ratio.
3) bead and solid-liquid ratio are 0.6:1, then start to grind using vertical grinding machine, abrasive grains are less than up to 95%
5 microns.
2 5.5% ferulic acid Physcion suspending agent of embodiment (+0.5% Physcion of 5% trans-ferulaic acid)
Preparation
Formula:The original powder 5% (wherein Physcion mass content is 10%) of Physcion, trans-ferulaic acid original powder
5.05% (wherein trans-ferulic content is 99%), sodium lignin sulfonate 6%, glycerine 5%, xanthans 0.1%, aluminium-magnesium silicate
0.2%, potassium sorbate 0.5%, antifoaming agent 0.3%, water complements to 100%.
The preparation method is the same as that of Example 1.
3 5.1% trans-ferulaic acid Physcion water dispersible granules (+0.1% rheum officinale of 5% trans-ferulaic acid of embodiment
Plain methyl ether) preparation
Formula:The original powder 1% of Physcion (wherein Physcion mass content is 10%);Ferulic acid original powder
5.05% (wherein trans-ferulic content is 99%);Dispersion moisture agent HRLD series 11%;Ammonium sulfate 30%;Antifoaming agent 5%;
Cornstarch 20%;Fine particle calcium carbonate supplies 100%.
Preparation method:Each raw material is taken according to the ratio, it puts and is mixed in a mixer, pass through air-flow crushing after mixing
Machine crush, mixed material low-grade fever, add suitable water be granulated, dried, sieve to get.
4 5.5% trans-ferulaic acid Physcion water dispersible granules (+0.5% rheum officinale of 5% trans-ferulaic acid of embodiment
Plain methyl ether) preparation
Formula:The original powder 5% of Physcion (wherein Physcion mass content is 10%);Trans-ferulaic acid original powder
5.05% (wherein trans-ferulic content is 99%);Dispersion moisture agent HRLD series 15%;Ammonium sulfate 30%;Antifoaming agent 5%;
Cornstarch 20%;Fine particle calcium carbonate supplies 100%.
Preparation method is the same as embodiment 3.
Experimental example 1
5.1% trans-ferulaic acid Physcion suspending agent (+5% trans-ferulaic acid of 0.1% Physcion) is prevented
Pepper virus disease field control effectiveness test
1 test objective
Understand 5.1% trans-ferulaic acid Physcion to the control effect of pepper virus disease, dosage, using skill
Art and its safety.
2 experimental conditions
The selection of 2.1 subjects, crop and kind
Subjects:Pepper virus disease ([Cucumber mosaic virus (CMV), tobacco mosaic virus (TMV) (TMV), marmor upsilon
(PVY), alfalfa mosaic virus (AMV), capsicum mottle virus (PeMV) etc.;
Trial crops:Capsicum (rising star F1)
2.2 arable farmings and environmental condition
It tests and determines the cities and towns villages Tan Li Longzhou in Hainan Province Dingan County on January 11,4 days~2017 November in 2016
Carry out to foreign capsicum.
Capsicum variety is rising star F1, and capsicum transplants each cell on 4th in seedlings nursing plate nursery on October 3, November.
Experiment plot soil is laterite, and in the content of organic matter, fertilizer management is medium.
3 experimental designs and arrangement
3.1 medicament
3.1.1 test medicine
5.1% trans-ferulaic acid Physcion suspending agent prepared by embodiment 1.
3.1.2 comparison medicament
2% Ningnanmycin aqua, Deqiang Biology Co., Ltd. are commercially available.
0.1% Physcion aqua, with differing only in without trans-ferulaic acid for embodiment 1.
5% trans-ferulaic acid microemulsion, with differing only in without Physcion for embodiment 1.
Each medicament extension rate see the table below 1.
Table 1
3.2 plot areas and repetition
Plot area (or plant number):10m2Left and right (protecting field).
Number of repetition:Often handle 4 repetitions.
3.3 application method
3.3.1 application method:Start dispenser in capsicum early stage.After above-mentioned medicament is prepared by design concentration, use is small
Type power driven sprayer is sprayed, and blade back blade face is uniformly squirted.Continuous spray 3 times, during which interval 7d, when spray, pay attention to wind direction, prevent
Only medicament interferes with each other.
3.3.2 spraying equipment:Prosperous standing grain 3WBD-16XX reflow types electric intelligent sprayer, metered flow 2.6L/min, work
Pressure 0.2-0.4MPa.
3.3.3 spraying time and number
It tests and by each processing design quantity apply for the first time in vegetative growth phase, viral early stage (December 21)
Medicine, second of dispenser of (December 28) progress after 7 days, (January 4) progress third time dispenser after 7 days.
3.3.4 capacity is used
By the water consumption preparating liquid of 900 liters/hectare.
3.3.5 the medicament data of other pest and disease damages is prevented
It is managed everywhere during experiment and does not apply other any sterilizations, insecticide, other management measures are consistent.
4 investigation, record and measurement method
4.1 meteorological and soil informations
4.1.1 meteorological data
First time dispenser (December 21) minimum temperature is 16.0 DEG C, and maximum temperature is 23.0 DEG C, rainfall 0mm,
Sunshine time is 12 hours.
Second of dispenser (December 28) minimum temperature is 16.0 DEG C, and maximum temperature is 23.0 DEG C, rainfall 0mm,
Sunshine time is 12 hours.
Third time dispenser is that (January 4) minimum temperature of next year is 16.0 DEG C, and maximum temperature is 23.0 DEG C, and rainfall is
0mm, sunshine time are 12 hours.
Mean temperature is 28.89 DEG C, rainfall 94.00mm during experiment, sunshine time 300 hours.
4.1.2 soil information
Experimental field soil is laterite, and middle fertility, irrigation and drainage are good, do not apply fertilizer during experiment.
4.2 investigation methods, time and number
4.2.1 control time and number
(January 11) Investigate incidence, disease index and preventive effect 7 days after third time medicine.
4.2.2 investigation method
According to pepper plant damage symptom grading, as unit of strain, per cell random searching 10 plants (all investigation),
Record the total strain number of investigation, diseased plant number at different levels.
0 grade:Without any symptom;
1 grade:The bright arteries and veins of lobus cardiacus or light floral leaf;
3 grades:Lobus cardiacus and middle leaf piece floral leaf, sometimes blade there is necrotic plaque;
5 grades:Most blade floral leaves, a small number of leaf malformations, shrinkage, there is necrotic plaque or stem all in blade or stem sometimes
There is billet spot;
7 grades:Most leaf malformations, elongated or cane, vein generation system necrosis, plant are downgraded;
9 grades:Plant serious systemic floral leaf, deformity, or serious systemic necrosis, plant are obviously downgraded sometimes, in addition it is dead.
4.2.3 drug effect computational methods
Drug effect computational methods:
Disease index=[Σ (plant number × rating value of each disease grade)/(plant sum × highest level number) ]×
100 (1)
Control effect (%)=1 — [(CK0×PT1)/(CK1×PT0)]×100 (2)
In formula (2):CK0 is disease index before blank district dispenser;
CK1 is disease index after blank district dispenser;
PT0 is disease index before chemicals treatment district dispenser;
PT1 is disease index after chemicals treatment district dispenser.
The computational methods of synergistic function:
It include the expection preventive effect of the composition of two kinds of ingredients X and Y using Colby equation calculations:
X=is identical with composition to use the preventive effect that ingredient Physcion is observed under concentration
Y=is identical with composition to use the preventive effect that ingredient trans-ferulaic acid is observed under concentration
If the ratio of practical preventive effect and expected preventive effect is more than 1, mixture shows synergistic effect.
4.3 directly affect crop
To the influence situation of trial crops growth, leaf color after visual observations dispenser, examination medicament is to trial crops plant
Safety.During the test, each dosage processing of 5.1% trans-ferulaic acid Physcion suspending agent, gives birth to All ice strain
Length has no adverse effects, and plant strain growth is normal after dispenser.
4.4 influence other biological:Nothing.
4.4.1 to the influence of other pest and disease damages
Discovery is not apparent from during experiment.
4.4.2 to the influence of other non-target organisms
During experiment, it is not apparent from and finds that 5.1% trans-ferulaic acid Physcion suspending agent is non-to fields such as natural enemies
Target organisms have an impact.
5 results and analysis
Experimental result is shown in Table 2.
2 5.1% trans-ferulaic acid Physcion suspending agent of table prevents pepper virus disease field control effectiveness test result
5.1% trans-ferulaic acid Physcion suspending agent prevents pepper virus disease, preparation diluent 600,400,200
Times, 7 days after 3 medicines, preventive effect is followed successively by 52.63%, 71.89%, 75.42%;2% Ningnanmycin aqua of comparison medicament dilutes
200 times of preventive effects are 47.89%;It is 38.95% that 0.1% Physcion aqua, which dilutes 400 times of preventive effects, and statistical result shows:
5.1% trans-ferulaic acid Physcion suspending agent respectively handles preventive effect and is improved with the increase of dosage;In same 200 times of dilution
When 5.1% trans-ferulaic acid Physcion suspending agent its control effect be significantly higher than the prevention effect of 2% Ningnanmycin aqua
Fruit;In 400 times of same dilution, 5.1% trans-ferulaic acid Physcion suspending agent its control effect is also above 0.1% rheum officinale
The control effect of plain methyl ether aqua and 5% trans-ferulaic acid microemulsion.When diluting 400 times, practical preventive effect is 71.89%, with
It is expected that the ratio of 63.72 preventive effects is 1.13, show that ferulic acid is mixed with Physcion with synergistic function.
Above-mentioned test result shows the 5.1% trans-ferulaic acid Physcion suspending agent that embodiment 1 provides, at 3
Dosage is managed, can effectively prevent pepper virus disease, and generate without poisoning.It is recommended that in capsicum vegetative growth phase, 20 or so, blade, disease
Before the onset of viral disease or early stage is watered even spraying, and emphasis is sprayed on leaf surface, and entire capsicum breeding time uses 3 times, every time extremely
Be spaced 7 days less or more.It is 200~600 times of uses of preparation diluent to recommend dosage.
Experimental example 2
5.5% trans-ferulaic acid Physcion water dispersible granules (+0.5% Physcion of 5% trans-ferulaic acid)
Prevent powdery mildew of cucumber experiment
One, material to be tested
Reagent agent:5.5% trans-ferulaic acid Physcion water dispersible granules and blank pair prepared by embodiment 4
According to.
0.5% Physcion aqua, and differing only in without trans-ferulaic acid for embodiment 1, wherein activity at
It is 0.5% to divide the content of Physcion.
5% trans-ferulaic acid microemulsion, with differing only in without Physcion for embodiment 1.
For trying cucumber variety:Chang Chun Mi Ci
For trying strain:The fresh sick leaf of scientia Agricultura Sinica research institute vegetables and the plastic greenhouse picking of flowers research institute
Two, the preparation of sowing media:Vermiculite and turf Nutrition Soil are pressed 3:1 ratio is uniformly mixed, then high at 121 DEG C
Pressure sterilizing 2h.
Three, it sows:Experimental cultivar selects susceptible variety Chang Chun Mi Ci, seed to sterilize 10min through 5% liquor natrii hypochloritis
Afterwards, it is rinsed, is put into the culture dish for being lined with two layers of filter paper with clear water, then 28 DEG C of vernalization in constant incubator.Wait for that radicle is long
When to 0.5cm, it is sowed in the plastic seeding culturing alms bowl equipped with sowing media, per 1, alms bowl, 20~25 DEG C of warm indoor raising of seedling.
Four, the preparation of inoculation liquid:In the Powdery Mildew Epidemic phase, the cucumber disease leaf with typical powdery mildew scab is acquired, is brushed off
Old spore on blade face is placed in bottom and is covered in the ceramic whiteware disk of wet filter paper, and moisturizing is for 24 hours in 24 DEG C or so of room temperature.With clean hair
Brush takes the Fresh spores grown on blade in filling in the beaker of sterile water, then 1000 μ g/mL lauryl sodium sulfate are added dropwise
(SDS), up to spore suspension after high-speed stirred (1200r/min) 3~5min, it is dilute to count conidium number with blood counting chamber
It releases to inoculum density:105 spore/mL.
Five, test method:Experiment sets 1 concentration, and reagent agent is diluted to 1200 times of liquid respectively, is compared with clear water,
Each processing is set to be repeated three times, repeats 10 young plants every time.Spraying treatment is proceeded by the 1-2 leaf phases, is first sprayed with medicament before inoculation
Mist handles plant, and powdery mildew is inoculated with spray-on process after 1 hour.In 12~16h of dark moisturizing in 22~25 DEG C of greenhouse after inoculation.
After be transferred to 25~28 DEG C of daytime, 20 DEG C~22 DEG C of night, relative air humidity normal management in 85% or more greenhouse.
Six, Disease investigation and grade scale:Each morbidity for handling each single plant of investigation in 7-10 days after second of spray
Situation records diseased plant number and sick grade, calculates control effect, compare fungistatic effect of the rheum emodin to powdery mildew of various concentration.
The sick grade scale such as the following table 3 for surveying grade in greenhouse.
Table 3
Drug effect computational methods:
Disease index=[Σ (plant number × rating value of each disease grade)/(plant sum × highest level number) ]×
100 (3)
Control effect (%)=1 — [(CK0×PT1)/(CK1×PT0)]×100 (4)
In formula (4):CK0 is disease index before blank district dispenser;
CK1 is disease index after blank district dispenser;
PT0 is disease index before chemicals treatment district dispenser;
PT1 is disease index after chemicals treatment district dispenser.
The computational methods of synergistic function:
It include the expection preventive effect of the composition of two kinds of ingredients X and Y using Colby equation calculations:
X=is identical with composition to use the preventive effect that ingredient Physcion is observed under concentration
Y=is identical with composition to use the preventive effect that ingredient trans-ferulaic acid is observed under concentration
If the ratio of practical preventive effect and expected preventive effect is more than 1, mixture shows synergistic effect.
Seven, experimental result is shown in Table 4.
4 5.5% trans-ferulaic acid Physcion of table prevents powdery mildew of cucumber test result
Experimental example 2 the result shows that, 5.5% trans-ferulaic acid Physcion dilutes 1200 times of prevention powderys mildew of cucumber
Effect is up to 90.7%, is better than all processing;0.5% Physcion aqua dilutes 1200 times of control effects and takes second place, and is also more than
65.9%, the effect that 5% 1200 times of trans-ferulaic acid micro emulsion dilution agent prevents powdery mildew of cucumber is 57.1%.Trans-ferulaic acid and
Physcion is re-dubbed, and the effect of prevention powdery mildew of cucumber is 90.7%, and the theoretical preventive effect of mixture is 85.37%, association
It is 1.06 with coefficient, there is synergistic function.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (9)
1. a kind of composition pesticide, active constituent includes trans-ferulaic acid and Physcion.
2. composition pesticide according to claim 1, which is characterized in that its active constituent trans-ferulaic acid and rheum emodin first
The mass ratio of ether is 1:900~900:1, preferably 50:1~1:40;Further preferably 55:1~50:10;More preferably 50:
1~50:5.
3. composition pesticide according to claim 1, which is characterized in that its active constituent trans-ferulaic acid and rheum emodin first
The mass ratio of ether is 50:1 or 50:5.
4. a kind of pesticide, separately made by any one of the claim 1-3 composition pesticides, or any by claim 1-3
The item composition pesticide is made with the available carrier of pesticide field or auxiliary agent;
Preferably, the pesticide is suspending agent, water dispersible granules, emulsifiable concentrate, microemulsion, aqueous emulsion, soluble concentrate, wettable powder
Agent or granule.
5. pesticide according to claim 4, which is characterized in that the matter of trans-ferulaic acid and Physcion in the pesticide
The content of the sum of amount is 0.1-90%, preferably 4-6%, further preferably 5-5.5%.
6. pesticide according to claim 4, which is characterized in that trans-ferulaic acid and rheum emodin first in the composition pesticide
The content of the quality sum of ether is 5.1%;The content of wherein preferred trans-ferulaic acid and Physcion be respectively 5% and
0.1%;
Alternatively, the content of the quality sum of trans-ferulaic acid and Physcion is 5.5% in the composition pesticide;It is wherein excellent
It is respectively 5% and 0.5% to select the content of trans-ferulaic acid and Physcion.
7. a kind of pesticide, which is characterized in that the pesticide is suspending agent, including trans-ferulaic acid 5%, Physcion 0.1%,
Sodium lignin sulfonate 3%, glycerine 5%, xanthans 0.2%, aluminium-magnesium silicate 0.4%, potassium sorbate 0.5%, antifoaming agent 0.3%,
Water complements to 100%;
Or the pesticide includes:Trans-ferulaic acid 5%, Physcion 0.5%, sodium lignin sulfonate 6%, glycerine 5% are yellow
Virgin rubber 0.1%, aluminium-magnesium silicate 0.2%, potassium sorbate 0.5%, antifoaming agent 0.3%, water complements to 100%;
Preferably, the pesticide each component grain size is less than or equal to 5 microns.
8. a kind of pesticide, which is characterized in that the pesticide is water dispersible granules, including:Trans-ferulaic acid 5%, Physcion
0.1%, dispersion moisture agent HRLD series 11%;Ammonium sulfate 30%;Antifoaming agent 5%;Cornstarch 20%;Fine particle calcium carbonate supplies 100%;
Or the pesticide includes:Trans-ferulaic acid 5%, Physcion 0.5%, dispersion moisture agent HRLD series 15%;Ammonium sulfate
30%;Antifoaming agent 5%;Cornstarch 20%;Fine particle calcium carbonate supplies 100%.
9. any one of any one of the claim 1-3 composition pesticides or claim 4-8 pesticides are anti-in plant virus
The application controlled;
Preferably, controlling object includes tobacco, tomato, cucumber, capsicum, apple, rice, corn, wheat, potato, clover;
And/or
Preferably, the plant virus includes tobacco (floral leaf) virus, tomato virus, cucumber virus, capsicum virus, apple disease
Poison, Rice Virus, corn virus, Wheat Virus, potato (Y) virus, clover virus.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810646059.6A CN108684669A (en) | 2018-06-21 | 2018-06-21 | A kind of composition pesticide containing trans-ferulaic acid and Physcion and its application |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090412A (en) * | 2009-12-11 | 2011-06-15 | 南开大学 | Ferulic acid and ferulic acid derivative anti-phytoviral agents |
| CN102548418A (en) * | 2009-07-30 | 2012-07-04 | 玛珑生物创新有限公司 | Plant pathogen inhibitor combinations and methods of use |
-
2018
- 2018-06-21 CN CN201810646059.6A patent/CN108684669A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102548418A (en) * | 2009-07-30 | 2012-07-04 | 玛珑生物创新有限公司 | Plant pathogen inhibitor combinations and methods of use |
| CN102090412A (en) * | 2009-12-11 | 2011-06-15 | 南开大学 | Ferulic acid and ferulic acid derivative anti-phytoviral agents |
Non-Patent Citations (1)
| Title |
|---|
| 刘可越: "紫菀的化学成分研究", 《第六届全国药用植物与植物药学术研讨会》 * |
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