CN108668896B - Method for improving gerbera jamesonii root rot resistance - Google Patents
Method for improving gerbera jamesonii root rot resistance Download PDFInfo
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- CN108668896B CN108668896B CN201810373223.0A CN201810373223A CN108668896B CN 108668896 B CN108668896 B CN 108668896B CN 201810373223 A CN201810373223 A CN 201810373223A CN 108668896 B CN108668896 B CN 108668896B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention provides a method for improving the root rot resistance of an African daisy, which is an African daisy with India piriformis colonization by a root soaking method, improves the disease resistance of the African daisy root rot and has important significance for improving the commodity value of the African daisy. The method has the advantages of simple steps, strong operability and remarkable effect.
Description
Technical Field
The invention relates to the technical field of gerbera jamesonii seedling raising, in particular to a method for improving the resistance of gerbera jamesonii root rot.
Background
African daisy (Gerbera jamesoniiBolus) is one of five fresh cut flowers in the world, which belongs to perennial root herbaceous flowers. The flower bud has various colors, tall and straight flower branches, long flowering phase and difficult decay, is deeply loved by flower planters and consumers, and has extremely high economic value. In recent years, with the continuous expansion of planting areas, gerbera jamesonii diseases, particularly root rot, become important problems restricting the healthy development of the gerbera jamesonii industry in China.
The Piriformospora indica has wide host range and has wide effects of promoting the growth of host plants and enhancing the resistance of the plants to biotic and abiotic stresses. Inoculation of Piriformospora indica into the roots of African daisy, the beneficial effects of Piriformospora indica on plants may be an effective method to increase the resistance to root rot of African daisy.
Disclosure of Invention
The invention aims to provide a method for improving the African daisy root rot resistance, and further solves the problem caused by the African daisy root rot in production.
The technical scheme of the invention is as follows:
a method for improving the disease resistance of African daisy root rot includes preparing fermentation liquor of piriformospora indica, transplanting tissue culture seedling of African daisy, preparing fermentation liquor of cryptophyta, inoculating cryptophyta and observing the disease condition. It is characterized by increasing the resistance to root rot of gerbera jamesonii by inoculation with Piriformospora indica. The method has the advantages of simple steps, strong operability and remarkable effect.
The specific method comprises the following steps:
(1) preparation of Piriformospora indica fermentation broth
Picking 2 bacterial blocks with diameter of 5 mm from the edge of a Piriformospora indica colony stored in a rooting culture medium of a tissue culture seedling of gerbera jamesonii, placing the bacterial blocks in a PDA liquid culture medium, culturing for 3 days in a shaking table at 28 ℃ and 120 r/min, filtering by using single-layer gauze, collecting filtrate, adding water to dilute the filtrate to obtain the spore with concentration of 5 multiplied by 105Periplaneta indica fermentation broth per ml;
(2) piriformospora indica inoculation and transplantation of gerbera jamesonii tissue culture seedlings
After uncovering and hardening the tissue culture seedlings of the African daisy with good rooting for 2 days, cleaning the root system, soaking the African daisy in an Indian pyricularia indica fermentation liquid for 2 hours, and then transplanting the African daisy into a matrix;
(3) preparation of Phytophthora crypticola fermentation broth
Activating the cryptogeous epidermidis strain, and then selecting aerial hyphae on the edge of a colony to inoculate on a PDA solid culture medium; dark culturing at 28 deg.C for 5 days, selecting 2-3 blocks with colony edge diameter of 5 mm, culturing in PDA liquid culture medium at 28 deg.C and 120 r/min for 2 days, diluting with water to obtain spore concentration of 2 × 105Each/ml of cryptophytophthora crypticola fermentation broth;
(4) phytophthora cryptica inoculation and disease condition observation
The method comprises the steps of damaging roots of African chrysanthemum seedlings which are transplanted for 1 month and are inoculated with Piriformospora indica by using blades, soaking the African chrysanthemum seedlings in the African chrysanthemum fermentation liquor for 2 hours, taking out the African chrysanthemum seedlings, transplanting the African chrysanthemum seedlings into a flowerpot filled with humus, placing the flowerpot in a constant-temperature culture room at 25 +/-2 ℃ for culture, wherein the illumination intensity of the culture room is 1500 +/-200 lx, the illumination time is 12 h/d, observing the disease condition of the plants when the African chrysanthemum seedlings are inoculated on the 2 nd day, and using the African chrysanthemum seedlings which are not inoculated with Piriformospora indica as a control.
The invention has the following advantages:
1. the Piriformospora indica is preserved in a rooting culture medium of living plants, and has stronger colonization ability on hosts.
2. The invention can obviously improve the resistance of the gerbera jamesonii root rot and has higher application and economic value.
3. The method is simple to operate and high in feasibility.
Drawings
FIG. 1 Effect of Piriformospora indica on resistance to African daisy root rot A: gerbera jamesonii inoculated with pyricularia indica; b: african daisy, which is not inoculated with Piriformospora indica.
Detailed Description
The technical solution of the present invention is further illustrated below with reference to specific examples, but the present invention is not limited thereto.
Example 1
1. Preparation of Piriformospora indica fermentation broth
Picking 2 bacterial blocks with diameter of about 5 mm from the edge of a Piriformospora indica colony stored in a rooting culture medium of tissue culture seedlings of African daisy, placing the bacterial blocks in a 250 ml triangular flask filled with 100 ml of sterilized PDA liquid culture medium, culturing for 3 days in a shaking table at 28 ℃ and 120 r/min, filtering by using single-layer gauze, collecting filtrate, adding water to dilute to obtain the spore with concentration of 5 multiplied by 105Periplaneta indica fermentation broth per ml;
2. piriformospora indica inoculation and transplantation of gerbera jamesonii tissue culture seedlings
Uncovering and hardening the tissue culture seedlings of the 'exquisite' African daisy with good rooting for 2 days, cleaning the root systems, soaking the seedlings in Indian pyricularia indica fermentation liquor for 2 hours, and then transplanting the seedlings into a substrate;
3. preparation of Phytophthora crypticola fermentation broth
Activating the cryptogeous epidermidis strains, and then selecting the hyphae at the edges of the colonies to inoculate the hyphae on a PDA solid culture medium; dark culturing at 28 deg.C for 5 days, selecting 3 blocks with colony edge diameter of 5 mm, culturing in 250 ml triangular flask containing 100 ml sterilized PDA liquid culture medium at 28 deg.C and rotation speed of 120 r/min for 2 days, diluting with water to obtain spore concentration of 2 × 105Each/ml of cryptophytophthora crypticola fermentation broth;
4. phytophthora cryptica inoculation and disease condition observation
The 'exquisite' African chrysanthemum seedlings inoculated with Piriformospora indica and transplanted for 1 month are wounded by roots of blades and then soaked in the Phytophthora cryptica fermentation liquor for 2 hours, the seedlings are taken out and transplanted into a flowerpot filled with humus, the flowerpot is placed in a constant-temperature culture room with the temperature of 25 +/-2 ℃ for culture, the illumination intensity of the culture room is 1500 +/-200 lx, the illumination time is 12 h/d, and the disease condition of the plants starts to be observed on the 2 nd day of inoculation. Control was 'exquisite' african chrysanthemum seedlings not inoculated with pyricularia indica.
After 6 days of phytophthora crypthecogenica infection, the 'exquisite' african chrysanthemum treated with the piriformospora indica fermentation broth grew well (fig. 1A), while the control group of 'exquisite' african chrysanthemum began to lose water slightly at day 2, and at day 6, leaves lost water seriously, roots rotted and fallen off, and plants withered and died (fig. 1B).
It can thus be seen that: piriformospora indica can improve the disease resistance of gerbera root rot.
The African daisy with India pyricularia piriformis colonization is obtained by using the root soaking method, the disease resistance of the African daisy root rot is obviously improved, the significance is great for the production of the African daisy, and the African daisy has higher application and economic values.
Claims (1)
1. A method for improving the disease resistance of African daisy root rot is characterized in that: the method comprises the steps of preparing Piriformospora indica fermentation liquor, inoculating Piriformospora indica and transplanting African chrysanthemum tissue culture seedlings, preparing Phytophthora cryptica fermentation liquor, inoculating Phytophthora cryptica and observing the disease condition;
the method specifically comprises the following steps:
(1) preparation of Piriformospora indica fermentation broth
Picking 2 blocks with diameter of 5 mm from the edge of Piriformospora indica colony, placing in PDA liquid culture medium, culturing in shaking table at 28 deg.C and 120 r/min for 3 days, filtering with single-layer gauze, collecting filtrate, diluting with water to obtain spore with concentration of 5 × 105Periplaneta indica fermentation broth per ml;
(2) piriformospora indica inoculation and transplantation of gerbera jamesonii tissue culture seedlings
After uncovering and hardening the tissue culture seedlings of the African daisy with good rooting for 2 days, cleaning the root system, soaking the African daisy in an Indian pyricularia indica fermentation liquid for 2 hours, and then transplanting the African daisy into a matrix;
preparation of Phytophthora crypticola fermentation broth
Activating the cryptogeous epidermidis strain, and then selecting aerial hyphae on the edge of a colony to inoculate on a PDA solid culture medium; dark culturing at 28 deg.C for 5 days, selecting 2-3 blocks with colony edge diameter of 5 mm, culturing in PDA liquid culture medium at 28 deg.C and 120 r/min for 2 days, diluting with water to obtain spore with concentration of 2 × 105Each/ml of cryptophytophthora crypticola fermentation broth;
(4) phytophthora cryptica inoculation and disease condition observation
The method comprises the steps of damaging roots of African chrysanthemum seedlings which are transplanted for 1 month and are inoculated with Piriformospora indica by using blades, soaking the African chrysanthemum seedlings in the African chrysanthemum fermentation liquor for 2 hours, taking out the African chrysanthemum seedlings, transplanting the African chrysanthemum seedlings into a flowerpot filled with humus, placing the flowerpot in a constant-temperature culture room at 25 +/-2 ℃ for culture, wherein the illumination intensity of the culture room is 1500 +/-200 lx, the illumination time is 12 h/d, observing the disease condition of the plants when the African chrysanthemum seedlings are inoculated on the 2 nd day, and using the African chrysanthemum seedlings which are not inoculated with Piriformospora indica as a control.
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| 非洲菊根腐病致病机理及防治的研究进展;黄宇翔等;《福建农业学报》;20141231;第29卷(第6期);第609页第3节 * |
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