CN108668896A - A method of improving Africa Black Ostrich resistance - Google Patents
A method of improving Africa Black Ostrich resistance Download PDFInfo
- Publication number
- CN108668896A CN108668896A CN201810373223.0A CN201810373223A CN108668896A CN 108668896 A CN108668896 A CN 108668896A CN 201810373223 A CN201810373223 A CN 201810373223A CN 108668896 A CN108668896 A CN 108668896A
- Authority
- CN
- China
- Prior art keywords
- india
- zymotic fluid
- pyriform spore
- inoculation
- spore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of method improving Africa Black Ostrich resistance, obtains the African Chrysanthemum for having India's pyriform spore to colonize by soaking root method, improves Africa Black Ostrich disease resistance, be of great significance to the promotion of African Chrysanthemum plant commodity value.Step of the present invention is simple, operability is strong, significant effect.
Description
Technical field
The present invention relates to African Chrysanthemum seedling-raising technique field more particularly to a kind of methods improving Africa Black Ostrich resistance.
Background technology
African Chrysanthemum（Gerbera jamesoniiBolus）It is that one of five big Fresh Cutting flower of the world belongs to perennial herb flower
Grass.Its is in a great variety, and spray is tall and straight, and the florescence is long and is not easy to decay, deep to be liked by flower planting person and consumer, has high
Economic value.In recent years, with the continuous amplification of cultivated area, African Chrysanthemum disease especially root rot getting worse, it has also become
Restrict the important problem that China's African Chrysanthemum industry develops in a healthy way.
India's pyriform spore host range is wide, to its host plant have it is extensive promote growth and enhancing plant to biology and
The effect of abiotic stress resistance.India's pyriform spore is seeded to African Chrysanthemum root system, using India's pyriform spore to the beneficial of plant
Effect is likely to be the effective ways for improving Africa Black Ostrich resistance.
Invention content
The purpose of the present invention is to provide a kind of methods improving Africa Black Ostrich resistance, and then solve the upper Africa of production
The problem of chrysanthemum root rot is brought.
Technical scheme of the present invention：
A method of Africa Black Ostrich disease resistance being improved, including the preparation of India's pyriform spore zymotic fluid, African Chrysanthemum group
Train the transplanting of seedling, the preparation of hidden ground phytophthora zymotic fluid, the inoculation of hidden ground phytophthora and incidence observation.It is characterized in that, by connecing
Kind India pyriform spore improves the Resistance To Root Rot Disease of African Chrysanthemum.Step of the present invention is simple, operability is strong, significant effect.
Specific method is：
（1）The preparation of India's pyriform spore zymotic fluid
From the fungus block 2 for India a diameter of 5 mm of pyriform spore colony edge picking being stored in African Chrysanthemum tissue-cultured seedling root media
Block is placed in PDA liquid medium, and filter is collected in culture 3 days in 28 DEG C, the shaking table of 120 r/min after mono layer gauze filters
Liquid, it is 5 × 10 to be diluted with water and obtain spore concentration5India's pyriform spore zymotic fluid of a/ml；
（2）India's pyriform spore inoculation and the transplanting of African Chrysanthemum tissue-cultured seedling
The good African Chrysanthemum tissue-cultured seedling that will take root uncapped hardening after 2 days, cleans root system, impregnates 2 in India's pyriform spore zymotic fluid
H is transplanted into matrix later；
（3）The preparation of hidden ground phytophthora zymotic fluid
Hidden ground Mtr mutant is activated, then the aerial hyphae at picking colony edge is inoculated on PDA solid mediums；In 28 DEG C of trainings
Support 5 d of light culture in case, picking colony edge diameter is the fungus block 2-3 blocks of 5 mm in PDA liquid medium, is 28 in temperature
DEG C, rotating speed is culture 2 days in the shaking table of 120 r/min, and it is 2 × 10 to be diluted with water and obtain spore concentration5The hidden ground phytophthora of a/ml
Zymotic fluid；
（4）The phytophthora inoculation of hidden ground and incidence observation
It is soaked in hidden ground phytophthora zymotic fluid after the African Chrysanthemum seedling of 1 month inoculation India pyriform spore of transplanting is hindered root with blade
It 2 hours, after taking-up in transplanting to the flowerpot equipped with humus, is placed in 25 ± 2 DEG C of constant temperature incubation room and cultivates, culturing room's illumination
Intensity is 1500 ± 200lx, and light application time is 12 h/d, and inoculation starts to observe the incidence of plant on the 2nd day, not to be inoculated with print
Spend the African Chrysanthemum seedling of pyriform spore as a contrast.
The present invention has the following advantages：
1, India's pyriform spore used in the present invention is stored in plants root media, stronger to the colonization ability of host.
2, the present invention can significantly improve Africa Black Ostrich resistance, have higher application and economic value.
3, the present invention is easy to operate, and feasibility is strong.
Description of the drawings
Influence .A of Fig. 1 India pyriform spore to Africa Black Ostrich resistance：It is vaccinated with the African Chrysanthemum of India's pyriform spore；B：
It is not inoculated with the African Chrysanthemum of India's pyriform spore.
Specific implementation mode
The following further describes the technical solution of the present invention with reference to specific embodiments, but the present invention is not limited in
This.
Embodiment 1
1, the preparation of India's pyriform spore zymotic fluid
The bacterium for being about 5 mm from the India's pyriform spore colony edge picking diameter being stored in African Chrysanthemum tissue-cultured seedling root media
2 pieces of block is placed in the 250 ml triangular flasks equipped with the 100 ml PDA liquid mediums to sterilize, and at 28 DEG C, 120 r/min's shakes
Culture 3 days in bed, through mono layer gauze filtering and collecting filter liquid, it is 5 × 10 to be diluted with water and obtain spore concentration5The India of a/ml
Pyriform spore zymotic fluid；
2, the inoculation of India's pyriform spore and the transplanting of African Chrysanthemum tissue-cultured seedling
Good ' exquisite ' the African Chrysanthemum tissue-cultured seedling that will take root uncapped hardening after 2 days, root system was cleaned, in India's pyriform spore zymotic fluid
2 h are impregnated, are transplanted later into matrix；
3, the preparation of hidden ground phytophthora zymotic fluid
Hidden ground Mtr mutant is activated, then picking colony edge mycelium inoculation is on PDA solid mediums；In in 28 DEG C of incubators
5 d of light culture, picking colony edge diameter are 3 pieces of the fungus block of 5 mm in the 250 of the PDA liquid medium to sterilize equipped with 100 ml
It it is 28 DEG C in temperature, rotating speed is culture 2 days in the shaking table of 120 r/min, is diluted with water acquisition spore concentration in ml triangular flasks
It is 2 × 105The hidden ground phytophthora zymotic fluid of a/ml；
4, the inoculation of hidden ground phytophthora and incidence observation
Hidden ground phytophthora hair is soaked in after ' exquisite ' African Chrysanthemum seedling of 1 month inoculation India pyriform spore of transplanting is hindered root with blade
2 hours in zymotic fluid, after taking-up in transplanting to the flowerpot equipped with humus, it is placed in 25 ± 2 DEG C of constant temperature incubation room and cultivates, culture
Room intensity of illumination is 1500 ± 200lx, and light application time is 12 h/d, and inoculation starts to observe the incidence of plant on the 2nd day.With not
It is inoculated with ' exquisite ' the African Chrysanthemum seedling of India's pyriform spore as a contrast.
After hidden ground phytophthora infects 6 days, ' exquisite ' African Chrysanthemum well-grown of India's pyriform spore fermentation liquor treatment is used（Figure
1A）, and control group ' exquisite ' African Chrysanthemum starts slight dehydration occur on the 2nd day, when the 6th day, leaves water loss is serious, butt rot
Fall off, plant wilt and it is dead（Figure 1B）.
It can be seen that：India's pyriform spore can improve the disease resistance of Africa Black Ostrich.
The present invention obtains the African Chrysanthemum for having India's pyriform spore to colonize using leaching root method, and it is disease-resistant to significantly improve Africa Black Ostrich
Property, it is significant for African Chrysanthemum production, there is higher application and economic value.
Claims (2)
1. a kind of method improving Africa Black Ostrich disease resistance, it is characterised in that：Method includes India's pyriform spore zymotic fluid
It prepares, India's pyriform spore inoculation and the transplanting of African Chrysanthemum tissue-cultured seedling, the preparation of hidden ground phytophthora zymotic fluid, the inoculation of hidden ground phytophthora and hair
State of an illness condition is observed.
2. a kind of method improving Africa Black Ostrich disease resistance according to claim 1, it is characterised in that：It specifically includes
Following steps：
（1）The preparation of India's pyriform spore zymotic fluid
From 2 pieces of the fungus block of India a diameter of 5 mm of pyriform spore colony edge picking, be placed in PDA liquid medium, 28 DEG C,
Culture 3 days in the shaking table of 120 r/min, through mono layer gauze filtering and collecting filter liquid, be diluted with water obtain spore concentration be 5 ×
105India's pyriform spore zymotic fluid of a/ml；
（2）India's pyriform spore inoculation and the transplanting of African Chrysanthemum tissue-cultured seedling
The good African Chrysanthemum tissue-cultured seedling that will take root uncapped hardening after 2 days, cleans root system, impregnates 2 in India's pyriform spore zymotic fluid
H is transplanted into matrix later；
The preparation of hidden ground phytophthora zymotic fluid
Hidden ground Mtr mutant is activated, then the aerial hyphae at picking colony edge is inoculated on PDA solid mediums；In 28 DEG C of trainings
Support case in 5 d of light culture, picking colony edge diameter be 5 mm fungus block 2-3 blocks in PDA liquid medium, temperature be 28
DEG C, rotating speed is culture 2 days in the shaking table of 120 r/min, and it is 2 × 10 to be diluted with water and obtain spore concentration5The hidden ground phytophthora of a/ml
Zymotic fluid；
（4）The phytophthora inoculation of hidden ground and incidence observation
It is soaked in hidden ground phytophthora zymotic fluid after the African Chrysanthemum seedling of 1 month inoculation India pyriform spore of transplanting is hindered root with blade
It 2 hours, after taking-up in transplanting to the flowerpot equipped with humus, is placed in 25 ± 2 DEG C of constant temperature incubation room and cultivates, culturing room's illumination
Intensity is 1500 ± 200lx, and light application time is 12 h/d, and inoculation starts to observe the incidence of plant on the 2nd day, not to be inoculated with print
Spend the African Chrysanthemum seedling of pyriform spore as a contrast.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810373223.0A CN108668896B (en) | 2018-04-24 | 2018-04-24 | Method for improving gerbera jamesonii root rot resistance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810373223.0A CN108668896B (en) | 2018-04-24 | 2018-04-24 | Method for improving gerbera jamesonii root rot resistance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108668896A true CN108668896A (en) | 2018-10-19 |
| CN108668896B CN108668896B (en) | 2021-08-31 |
Family
ID=63802115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810373223.0A Active CN108668896B (en) | 2018-04-24 | 2018-04-24 | Method for improving gerbera jamesonii root rot resistance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108668896B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235665A (en) * | 2019-07-12 | 2019-09-17 | 三明市农业科学研究院 | A kind of method of biological control Africa Black Ostrich |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107155896A (en) * | 2017-07-12 | 2017-09-15 | 福建农林大学 | A kind of method for promoting African Chrysanthemum tissue culture to transplant seedling rooting |
-
2018
- 2018-04-24 CN CN201810373223.0A patent/CN108668896B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107155896A (en) * | 2017-07-12 | 2017-09-15 | 福建农林大学 | A kind of method for promoting African Chrysanthemum tissue culture to transplant seedling rooting |
Non-Patent Citations (1)
| Title |
|---|
| 黄宇翔等: "非洲菊根腐病致病机理及防治的研究进展", 《福建农业学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235665A (en) * | 2019-07-12 | 2019-09-17 | 三明市农业科学研究院 | A kind of method of biological control Africa Black Ostrich |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108668896B (en) | 2021-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI377905B (en) | Method for incubating fruiting bodies of antrodia cinnamomea | |
| CN107155896B (en) | A method of promote African Chrysanthemum tissue culture to transplant seedling rooting | |
| CN106576910B (en) | Culture method of in-vitro sterile host plant-mycorrhizal edible fungus symbiotic seedling | |
| CN109749943A (en) | A kind of Trichoderma asperellum and its application | |
| CN103103152B (en) | Corallococcus coralloides and application thereof | |
| CN107988087B (en) | Blueberry endophytic fungus with growth promoting effect and application thereof | |
| CN106434349B (en) | A method of mycorrhizal fungi is acquired using sterile blueberry tissue culture seedling | |
| CN104845892A (en) | R.vinctus and application thereof in promoting aquilaria plants to produce agilawood | |
| CN103146609B (en) | Pseudomonas fluorescens and method for preventing phytophthora capsici thereby | |
| CN102845218A (en) | Original ecological separation method of cordyceps sinensis hirsutella sinensis | |
| CN109355200A (en) | A kind of isolation and purification method of the small bolete mycelia of sky handle | |
| CN108668896A (en) | A method of improving Africa Black Ostrich resistance | |
| CN117305130A (en) | Tobacco endophytic fungus 001I-21 with remarkable drought resistance effect and application thereof | |
| CN103182726B (en) | A kind of decorative wood material and preparation method thereof | |
| CN104126508B (en) | The method of the quick Mycorrhizal of a kind of orchid aseptic seedling | |
| Chellappan et al. | Multiplication of arbuscular mycorrhizal fungi on roots | |
| CN107810679B (en) | Method for improving germination rate of longan seeds stored at room temperature for short term | |
| CN109197379A (en) | A kind of efficient monospore expanding propagation method of arbuscular mycorrhizal fungi | |
| CN113930373A (en) | Method for preserving pathogenic bacteria of tobacco bacterial wilt, namely ralstonia solanacearum | |
| CN110235781A (en) | Clematis Blekitny Aniol tissue cultures and Regeneration System | |
| CN104920066B (en) | The method for improving host of Cordyceps sinensis infection rate | |
| CN108203695B (en) | Rhododendron mycorrhizal fungi functional strain and application thereof | |
| Janardhanan et al. | Axenic culture of a vesicular-arbuscular mycorrhizal fungus | |
| CN105238697A (en) | Peony endophytic fungi and process for producing paeonol with peony endophytic fungi | |
| CN116640670B (en) | Purple flower and sycamore endophytic fungus with growth promoting effect and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |