CN108651579A - The preparation method of Cordyceps militaris chiffon cake - Google Patents
The preparation method of Cordyceps militaris chiffon cake Download PDFInfo
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- CN108651579A CN108651579A CN201810797458.2A CN201810797458A CN108651579A CN 108651579 A CN108651579 A CN 108651579A CN 201810797458 A CN201810797458 A CN 201810797458A CN 108651579 A CN108651579 A CN 108651579A
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- cordyceps militaris
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
- A21D13/06—Products with modified nutritive value, e.g. with modified starch content
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/34—Animal material
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/36—Vegetable material
Abstract
The invention discloses a kind of preparation methods of Cordyceps militaris chiffon cake, wherein includes the following steps：Step 1, the component for weighing following parts by weight：Egg 40 60, flour 15 20, fruiting bodies of cordyceps militaris powder 23, Cordyceps militaris bacterium solution 10 12 and edible oil 7 12, sugared at least one of 10 30 and lemon juice 15；Step 2 mixes after dismissing egg with remaining ingredient, obtains cake paste；Step 3 toasts the sizing of gained cake paste to get the Cordyceps militaris chiffon cake.The present invention replaces part flour by the way that suitable fruiting bodies of cordyceps militaris powder is added, milk is replaced with Cordyceps militaris bacterium solution, and optimizing raw material formula, integration of drinking and medicinal herbs, made characteristics of cake out is higher, and cake can not only be made to have peculiar flavour, also has the healthcare functions such as certain antibacterial, anti-inflammatory and adjusting immunity, establishing criteria evaluation table scores to the color and luster of cake, fragrance, appearance, fluffy degree, mouth feel score, and gross score and indices score are above plain cake.
Description
Technical field
The present invention relates to food processing technology field, more particularly to a kind of preparation method of Cordyceps militaris chiffon cake.
Background technology
Cordyceps militaris also known as Cordceps militaris, be Ascomycota, meat seat mesh, Clavicipitaceae, Cordyceps type sepecies, Cordyceps militaris generation
Criticality is distributed, and natural resources quantity is seldom, and presently mainly artificial culture obtains, and a large amount of cordycepin, worm are contained in Cordceps militaris
Careless element has the function of to adjust whole body, improves the medical health care functions such as immunocompetence, anti-aging, antimicrobial antiphlogistic, has caused the country
The great attention of outer expert has the much health products based on cordycepin, health food, cosmetics, drug and launches.North
The healthcare function ingredient of cordyceps sinensis not only has cordycepin, and also Polysaccharides in Cultured Cordyceps militaris is the generally acknowledged human immunity reinforcing agent of International Medical.
Although cordycepin is applied in various aspects, it has not been found that the application in terms of dessert.
Chiffon cake is a dessert, belongs to spongecake type, and making raw material mainly has rape oil, egg, sugar, face
Powder, hair powder etc., but due to lack butter cake strong fragrance, chiffon cake usually require thick taste juice such as milk or
In addition the dispensings such as chocolate, fruit.Traditional cake is made of butter, since rape oil is easy to get blisters unlike butter,
It needs wearing or featuring stage armour egg white to break into foam-like, make the dosage that flour ferments and addition is oily with hair powder, to provide enough air
The volume of cake is supported, it is done so that cake out or harder, not soft enough, and compare heavy oil, it tastes
It is easy greasy to be also unfavorable for health.
Invention content
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of production methods of Cordyceps militaris chiffon cake, can make the relative wind of making
Cake is soft fresh and tender and with healthcare functions such as antibacterial, anti-inflammatory and adjusting immune functions.
It is a still further object of the present invention to provide a kind of production methods of Cordyceps militaris chiffon cake, can improve chiffon cake
Shelf-life.
It is a still further object of the present invention to provide a kind of Cordyceps militaris chiffon cake production methods, and cake is made to have cordyceps fresh
Fragrance road.
It is a still further object of the present invention to provide a kind of Cordyceps militaris chiffon cake formula containing integration of drinking and medicinal herbs substance, makes egg
Cake has certain healthcare function.
It is a still further object of the present invention to provide a kind of chiffon cakes with Cordyceps militaris composition, are extended compared with plain cake
Shelf-life.
In order to realize these purposes and other advantages according to the present invention, a kind of preparation of Cordyceps militaris chiffon cake is provided
Method, wherein include the following steps：
Step 1, the component for weighing following parts by weight：Egg 40-60, flour 15-20, fruiting bodies of cordyceps militaris powder 2-3, pupa worm
Careless bacterium solution 10-12, edible oil 7-12, sugar 10-30 and lemon juice 1-5；
Step 2 mixes after dismissing egg with remaining ingredient, obtains cake paste；
Step 3 toasts the sizing of gained cake paste to get the Cordyceps militaris chiffon cake.
Preferably, step 1 includes the component of following parts by weight：Egg 45-55, flour 17.6, fruiting bodies of cordyceps militaris
Powder 2.4, Cordyceps militaris bacterium solution 11, edible oil 9, sugar 15-20 and lemon juice 1-5.
Preferably, the specific preparation method of cake paste is in step 2：Egg is divided into egg white and yolk, by gained egg
It is clear to beat foaming, divide in whipping process 2-4 times and lemon juice and sugar is added, obtains protein paste；Yolk, flour, Cordyceps militaris is real
Body powder, Cordyceps militaris bacterium solution, edible oil mixing turn, and obtain egg yolk paste, gained protein paste is mixed with egg yolk paste to get the egg
Cake is pasted.
Preferably, the Cordyceps militaris daughter bacteria liquid is to ferment to obtain by trollflower pharmacological property culture medium inoculated cordyceps militaris link bacterial strain,
Wherein, the trollflower pharmacological property culture medium is obtained by the trollflower Aqueous extracts that mass percent 3-8% is added in basal medium.
Preferably, the basal medium includes the component of following parts by weight：150-250 parts of potato, wheat bran 15-25
Part, 10-20 parts of niblet, 15-25 parts of glucose, 3-8 parts of peptone and 800-1200 parts of distilled water；The trollflower water carries
Liquid additional proportion is the 7% of basic culture medium quality.
Preferably, the trollflower water body liquid is mixed extraction with distilled water by trollflower and obtained, and extracting method is temperature
Gradient extraction method, specifically includes following steps：
Step 1.1 mixes the trollflower after drying and crushing with the distilled water of 5-7 times of volume, and temperature keeps 42-45 Celsius
Degree, extraction time are 2-3 hours, are separated by filtration and respectively obtain the first extracting solution and first dregs of a decoction；
Step 1.2 mixes first dregs of a decoction with the distilled water of 3-5 times of volume, and temperature is kept for 60-65 degrees Celsius, when extraction
Between be 4-6 hours, be separated by filtration to obtain the second extracting solution and second dregs of a decoction；
Step 1.3 mixes second dregs of a decoction with 2-3 times of volume distilled water, boils 10-15 minutes, is separated by filtration to obtain
Three extracting solutions and the third dregs of a decoction；
Step 1.4 carries the mixing of the first extracting solution, the second extracting solution and third extracting solution to get the trollflower water
Liquid.
Preferably, the fruiting bodies of cordyceps militaris powder is obtained by fruiting bodies of cordyceps militaris drying and crushing.
Preferably, the fruiting bodies of cordyceps militaris is obtained by Cordyceps militaris solid state cultivation medium culture, wherein the pupa worm
Careless solid state cultivation culture medium includes the component of following parts by weight：Cooked rice 40-60, wheat bran 15-25, dried silkworm chrysalis meal 8-12, trollflower
Dregs of a decoction 5-10, sawdust 5-10, magnesium sulfate 0.05-0.15, wheat flour 5-7, glucose 1-3 and water 100-150, pH6-7.
Preferably, the Cordyceps militaris solid state cultivation culture medium includes the component of following parts by weight：Cooked rice 50, wheat bran
20, pupa powder 10, the trollflower dregs of a decoction 6, sawdust 6, magnesium sulfate 0.1, wheat flour 6, glucose 2 and water 120, pH6-7.
Preferably, the cultural method of fruiting bodies of cordyceps militaris is：The Cordyceps militaris solid state cultivation culture medium is bottled to 1/2
Full, sealing, 0.15MPa high pressure sterilization 2h are inoculated with 25 DEG C of dark situation cultures after cordyceps mycelium, and mycelia, which is covered with, moves to light
Place, 15 DEG C -25 DEG C are continued culture 25-40 days to there is orange red stroma to be formed and grown to 30-66mm high sub to get the Cordyceps militaris
Entity.
The present invention includes at least following advantageous effect：
The present invention provides a kind of Cordyceps militaris chiffon cake, and suitable pupa worm is added in the basic components for making chiffon cake
Grass seed entity powder replaces part flour, replaces milk, and optimizing raw material formula with Cordyceps militaris bacterium solution, cake can not only be made to have
The healthcare functions such as antibacterial, anti-inflammatory and adjusting immunity, additionally it is possible to so that the chiffon cake made has unique fragrance,
Without fishy smell, and floridity, fluffy degree is good, and cake is soft fresh and tender and non-greasy, and excellent taste, establishing criteria evaluation table is to cake
Color and luster, fragrance, appearance, fluffy degree, mouth feel score score, gross score and indices score are obviously higher than common relative
Wind cake.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific implementation mode
The present invention is described in further detail below, to enable those skilled in the art being capable of evidence with reference to specification word
To implement.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded one or more
The presence or addition of a other elements or combinations thereof.
The present invention provides a kind of preparation method of Cordyceps militaris chiffon cake, wherein includes the following steps：
Step 1, the component for weighing following parts by weight：Egg 40-60, flour 15-20, fruiting bodies of cordyceps militaris powder 2-3, pupa worm
Careless bacterium solution 10-12, edible oil 7-12, sugar 10-30 and lemon juice 1-5.
Step 2 mixes after dismissing egg with remaining ingredient, obtains cake paste.
Step 3 toasts the sizing of gained cake paste to get the Cordyceps militaris chiffon cake.
In the program, portion is replaced by the way that suitable fruiting bodies of cordyceps militaris powder is added in the basic components for making chiffon cake
Facet powder replaces milk, and optimizing raw material formula with Cordyceps militaris bacterium solution, and cake can not only be made to have antibacterial, anti-inflammatory and adjusting
The healthcare functions such as immunity, additionally it is possible to improve so that the chiffon cake made has unique fragrance, no fishy smell, and face
Color floridity, fluffy degree is good, and cake is soft fresh and tender and non-greasy, excellent taste, color and luster, perfume (or spice) of the establishing criteria evaluation table to cake
Taste, appearance, fluffy degree, mouth feel score score, and gross score and indices score are above plain cake.
In one preferred embodiment, step 1 includes the component of following parts by weight：Egg 45-55, flour 17.6, Cordyceps militaris
Fructification powder 2.4, Cordyceps militaris bacterium solution 11, edible oil 9, sugar 15-20 and lemon juice 1-5.The program is the Cordyceps militaris relative wind egg
The optimization formula of cake.Egg is specially the amount of an egg.Sugar is white granulated sugar, soft white sugar etc..
In one preferred embodiment, the specific preparation method of cake paste is in step 2：Egg is divided into egg white and yolk, it will
Gained egg white beats foaming, divides in whipping process 2-4 times and lemon juice and sugar is added, obtain protein paste；By yolk, flour, pupa worm
Grass seed entity powder, Cordyceps militaris bacterium solution, edible oil mixing turn, and obtain egg yolk paste, gained protein paste is mixed with egg yolk paste to get
The cake paste.
In one preferred embodiment, the Cordyceps militaris daughter bacteria liquid is to be fermented by trollflower pharmacological property culture medium inoculated cordyceps militaris link bacterial strain
It obtains, wherein the trollflower pharmacological property culture medium is by being added the trollflower Aqueous extracts of mass percent 3-8% in basal medium
It obtains.
In the program, by the way that a certain amount of trollflower Aqueous extracts are added in Cordyceps militaris basal medium, it can not only carry
The content of cordycepin in high cordyceps mycelium, by measuring, cordycepin content can reach in cordyceps mycelium
14.1470mg/g or more, content are at least 2 times or more of blank control group, while can make the cordyceps mycelium of gained
Fungistatic effect be significantly increased, not only directly enhance the nutritional quality of cake, but also any preservative can not added
Under the premise of significantly improve room temperature shelf-life of cake.Cake is made using Cordyceps militaris zymotic fluid obtained by the culture of trollflower concoction,
Using and the effect of played integration of drinking and medicinal herbs.
In one preferred embodiment, the basal medium includes the component of following parts by weight：150-250 parts of potato, wheat bran
800-1200 parts of 15-25 parts, 10-20 parts of niblet, 15-25 parts of glucose, 3-8 parts of peptone and distilled water；The lily feet
Flower Aqueous extracts additional proportion is the 7% of basic culture medium quality.
In the program, cordycepin content value is higher when trollflower Aqueous extracts additional proportion is the 7% of basic culture medium quality.
In one preferred embodiment, the trollflower water body liquid is mixed extraction with distilled water by trollflower and obtained, extracting method
For temperature gradient extraction method, following steps are specifically included：
Step 1.1 mixes the trollflower after drying and crushing with the distilled water of 5-7 times of volume, and temperature keeps 42-45 Celsius
Degree, extraction time are 2-3 hours, are separated by filtration and respectively obtain the first extracting solution and first dregs of a decoction.
Step 1.2 mixes first dregs of a decoction with the distilled water of 3-5 times of volume, and temperature is kept for 60-65 degrees Celsius, when extraction
Between be 4-6 hours, be separated by filtration to obtain the second extracting solution and second dregs of a decoction.
Step 1.3 mixes second dregs of a decoction with 2-3 times of volume distilled water, boils 10-15 minutes, is separated by filtration to obtain
Three extracting solutions and the third dregs of a decoction.
Step 1.4 carries the mixing of the first extracting solution, the second extracting solution and third extracting solution to get the trollflower water
Liquid.
It is raw in trollflower Aqueous extracts relative to the method routinely boiled by temperature gradient step by step arithmetic method in the program
Alkaloids content, flavonoids bioactive substance content significantly improve, and bioactive substance recovery rate improves 12%.
In one preferred embodiment, the fruiting bodies of cordyceps militaris powder is obtained by fruiting bodies of cordyceps militaris drying and crushing.
In one preferred embodiment, the fruiting bodies of cordyceps militaris is obtained by Cordyceps militaris solid state cultivation medium culture, wherein institute
State the component that Cordyceps militaris solid state cultivation culture medium includes following parts by weight：Cooked rice 40-60, wheat bran 15-25, dried silkworm chrysalis meal 8-12,
Trollflower dregs of a decoction 5-10, sawdust 5-10, magnesium sulfate 0.05-0.15, wheat flour 5-7, glucose 1-3 and water 100-150, pH6-
7。
In one preferred embodiment, the Cordyceps militaris solid state cultivation culture medium includes the component of following parts by weight：Cooked rice 50,
Wheat bran 20, dried silkworm chrysalis meal 10, the trollflower dregs of a decoction 6, sawdust 6, magnesium sulfate 0.1, wheat flour 6, glucose 2 and water 120, pH6-7.
In one preferred embodiment, the cultural method of fruiting bodies of cordyceps militaris is：The Cordyceps militaris solid state cultivation culture medium is filled
Bottle is inoculated with 25 DEG C of dark situation cultures after cordyceps mycelium to 1/2 full, sealing, 0.15MPa high pressure sterilization 2h, and mycelia covers with shifting
Locate to light, 15 DEG C -25 DEG C are continued to cultivate 25-40 days to there is orange red stroma to be formed and grown to 30-66mm high to get the pupa
Cordyceps militaris sporocarp.
Embodiment 1
Cake recipes are：50 grams of egg, 15 grams of flour, 2 grams of fruiting bodies of cordyceps militaris powder, 10 grams of Cordyceps militaris bacterium solution and salad
7 grams of oil, white granulated sugar sugared 10 grams and 2 milliliters of lemon juice.130 DEG C of preheating 15min of oven, are then put into oven with fire up and down by cake
145 DEG C of baking 50min.The 20-30 degrees Celsius of lower shelf-life of cake is 10-13 days.
Embodiment 2
Cake recipes are：60 grams of egg, 20 grams of flour, 3 grams of fruiting bodies of cordyceps militaris powder, 12 grams of Cordyceps militaris bacterium solution and corn
3 milliliters of 12 grams of oil, 30 grams of soft white sugar and lemon juice.Baking method such as embodiment 1.The 20-30 degrees Celsius of lower shelf-life of cake is 10-
13 days.
Embodiment 3
Cake recipes are：50 grams of egg, 17.6 grams of flour, 2.4 grams of fruiting bodies of cordyceps militaris powder, 11 grams of Cordyceps militaris bacterium solution, color
Draw 2 milliliters of 9 grams of oil, 17 grams of soft white sugar and lemon juice.Wherein, Cordyceps militaris daughter bacteria liquid and fruiting bodies of cordyceps militaris powder are by adding
The pharmacological property culture medium of trollflower Aqueous extracts ferments or culture obtains.Baking method such as embodiment 1.It is protected under 20-30 degrees Celsius of cake
The matter phase is 15-18 days.
Embodiment 4
Not plus the blank control group of fruiting bodies of cordyceps militaris powder and Cordyceps militaris bacterium solution.Baking method such as embodiment 1.20-30 takes the photograph
Family name's degree lower shelf-life is 3-7 days.
Cake is given 100 people to taste, establishing criteria evaluation table scores, successively to the color and luster of cake, fragrance, outer
Sight, fluffy degree, mouth feel score, every judging panel does not interfere with each other in scoring process.
The sensory evaluation standard of 1 Cordyceps militaris chiffon cake of table
2 are shown in Table to the scoring situation of the chiffon cake according to table 1
2 chiffon cake of table scoring statistics
It is scored and is counted according to table 2, fruiting bodies of cordyceps militaris powder is added in chiffon cake formula and substitute milk with Cordyceps militaris bacterium solution
1/2/3 embodiment obtained by the comprehensive scores such as chiffon cake mouthfeel, color and luster, fragrance and individual scores be superior to common relative wind egg
Cake, the shelf-life, also more common chiffon cake formula extended at least 3 days or more, wherein the sensory evaluation scores of scheme described in embodiment 3 are most
Height, close to full marks, the shelf-life, also longest, longest reached 18 days, were preferred plan.
Applicant carried out single factor experiment designs, determine each component optimal selection and optimum amount according to experiment and match
Than.
1) influence of the cordyceps mycelium freeze-dried powder to characteristics of cake
Because cordyceps mycelium freeze-dried powder weight is extremely light, and fishy smell is more apparent, therefore cordyceps mycelium freeze-dried powder additive amount
It is 100% addition 0%, 4%, 8%, 12%, 17%, 24% with flour additive amount.It is evaluated according to table 1, as a result such as table 3.
Influence of the 3 cordyceps mycelium freeze-dried powder of table to chiffon cake quality
2) influence of the fruiting bodies of cordyceps militaris ovendry power to chiffon cake quality
Because of fruiting bodies of cordyceps militaris ovendry power floridity, there is unique perfume, weight will not be very light, therefore fruiting bodies of cordyceps militaris dries
Dry powder additive amount is 100% addition 0%, 8%, 17%, 24%, 32% with flour additive amount.
It is evaluated according to table 1, as a result such as table 4.
Influence of the 4 fruiting bodies of cordyceps militaris ovendry power of table to chiffon cake quality
As known from Table 4, fruiting bodies of cordyceps militaris ovendry power compared with mycelium freeze-dried powder for, weight wants heavier, without fishy smell,
There is a kind of unique fragrance instead.Because its color is crocus, with the increase of its adding proportion, cake can integrally become gold therewith
Yellow, color very floridity.
From table 4, it can be seen that when fruiting bodies of cordyceps militaris ovendry power additive amount is 17%, score highest.Fruiting bodies of cordyceps militaris
When ovendry power additive amount ﹤ 8%, fresh Cordyceps militaris flavor is not enough to embody, the inadequate floridity of cake color, and elasticity is insufficient；Work as fructification
When ovendry power additive amount ﹥ 17%, Cordyceps militaris flavor gradually aggravates, but is also unlikely to be difficult to swallow；Mouthfeel is gradually dried, particle and
Harsh feeling is more shown in apparent.
2) influence of the Cordyceps militaris bacterium solution to chiffon cake quality is as a result, be shown in Table 5.
Influence of the 5 Cordyceps militaris bacterium solution of table to chiffon cake quality
The quality and milk of Cordyceps militaris bacterium solution (zymotic fluid and mycelial mixture) are similar, do not have cordyceps on smell
The fishy smell of powder there is no taste, and the result after addition has essentially no difference with blank control.
As can be seen from Table 5, it although the addition of Cordyceps militaris bacterium solution can increase the humidity of cake slightly, influences little.
Therefore on the whole, the additive amount of Cordyceps militaris bacterium solution has little effect cake, can substitute milk use.
4) influence of the Cordyceps militaris zymotic fluid to chiffon cake quality, is shown in Table 6.
Influence of the 6 Cordyceps militaris zymotic fluid of table to chiffon cake quality
Cordyceps militaris zymotic fluid is the remaining liq after isolating mycelium, diluter compared with bacterium solution, there is the fishy smell of Cordyceps militaris, but
It is less serious, therefore the addition of zymotic fluid can have certain influence to the humidity of chiffon cake.
As can be seen from Table 6, when Cordyceps militaris zymotic fluid additive amount is 24%, score highest.The sub- zymotic fluid addition of Cordyceps militaris
When measuring ﹤ 24%, Cordyceps militaris flavor is not enough to embody；As zymotic fluid additive amount ﹥ 24%, Cordyceps militaris flavor can embody, egg
The humidity of cake increases therewith；The color of cake surface also becomes more shallow therewith, but on the whole, the addition pair of Cordyceps militaris zymotic fluid
Cake mouthfeel and tissue effect are less apparent.
5) influence of Cordyceps militaris bacterium solution and fruiting bodies of cordyceps militaris ovendry power combination additive amount to characteristics of cake, is shown in Table 7.
7 Cordyceps militaris bacterium solution of table and fruiting bodies of cordyceps militaris ovendry power combine additive amount to characteristics of cake
Cordyceps militaris bacterium solution has little effect the mouthfeel and tissue of chiffon cake；Fruiting bodies of cordyceps militaris ovendry power has fresh pupa
The unique fragrance of cordyceps sinensis, and it has an impact the taste flavor and tissue of chiffon cake, therefore the two is combined.From table 7
As can be seen that Cordyceps militaris bacterium solution 100% replaces milk；When fruiting bodies of cordyceps militaris powder additive amount is 12%, score highest.This group
Relatively 4 groups of scores are generally higher before for cake, caused by the difference of each judging panel's taste, all in all the difference of score is
Achievement is good.
By experiment, determine that the best addition manner of Cordyceps militaris is bacterium solution and the combination of fructification powder, adding proportion
It is determined as：Cordyceps militaris bacterium solution additive amount is the 100% of milk；Fruiting bodies of cordyceps militaris powder additive amount is the 12% of flour.Therefore pupa worm
The optimum formula of careless chiffon cake is：Egg 1 (50g), fruiting bodies of cordyceps militaris powder 2.4g, flour 17.6g, bacterium solution 11g, oil
9g, sugar 17g, 3 milliliters of lemon juice.It obtains in the production process：Fire is 145 DEG C up and down, and the time is 50min (4 cun of chiffon cakes).
By the finished product that this formula toasts out, cake is in integrally golden yellow, color and luster floridity；Internal cellular tissue is good, without collapsing and larger
Stomata；Mouthfeel is careful fine and smooth and with the unique flavor of Cordyceps militaris.Both consumer's fine and smooth mouthfeel careful to chiffon cake had been met
Pursuit, and the effect of have both health care.
Embodiment 5
1) extraction will be mixed with 15 times of volume distilled water after 50 parts of trollflower drying and crushings, obtains trollflower Aqueous extracts；
2) basal medium is prepared according to following parts by weight of component：250 parts of potato, 25 parts of wheat bran, 20 parts of niblet, grape
1200 parts of 25 parts of sugar, 8 parts of peptone and distilled water, institute is added into basal medium according to the ratio of mass percent 7%
Trollflower Aqueous extracts are obtained, pharmacological property culture medium is obtained；
3) 100 milliliters of dosing culture medium in each triangular flask, sealed membrane sealing, labelled high pressure steam sterilization 121
DEG C, 20 minutes.Every bottle of inoculation cordyceps militaris link bacterial strain 8% of superclean bench is moved into after cooling, moves into 28 DEG C of shaking table, 150rpm cultures 9
It.Cordyceps militaris zymotic fluid and cordyceps mycelium are respectively obtained after fermentation.By calculating, mycelium content is
0.62g/ml。
4) it is 14.14mg/g to measure cordycepin content in mycelium.
Cordycepin content measures
4.1 sample pretreatment
Take out the zymotic fluid in shaking table, be transferred to centrifuge tube, abandon supernatant after 4000r/min centrifugations 15min, precipitation spend from
Sub- water washing 3 times is put into ultra low temperature freezer and freezes 48 hours, moves into freeze drier and dried to constant weight later, weighed.
4.2 extraction
The extraction of cordycepin uses ultrasonic extraction, carries out preliminary experiment first to determine the power of ultrasonic wave.After taking drying
Cordyceps mycelium, by material-water ratio 1：30 are added distilled water, extract 20min at ultrasonic power 400w, 4000r/min from
The heart 15 minutes, abandons precipitation, takes supernatant, obtain sample solution.
The measurement of 4.3 cordycepin contents
4.3.1 the determination of cordycepin standard curve
0.5mg cordycepin standard items are weighed, distilled water is added and is settled to 100ml, is configured to the mother liquor of 0.5mg/L, then
The cordycepin solution for preparing 0,1.0,2.0,3.0,4.0,5.0mg/L respectively again, measures its absorbance under the wavelength of 259nm
(A), with cordycepin concentration (C) for abscissa, it is that ordinate draws standard curve with absorbance (A), obtains normal equation：Y=
0.0878x+2.2837 (R2=0.9998).
4.3.2 in sample cordycepin content determination
It takes sample solution 1ml to dilute 100 times, measures absorbance when 259nm, bring normal equation into and find out concentration C.1ml
The amount S of cordycepin in sample0=C × V0×10(V0=1ml), the amount S=S of cordycepin in every part of sample0× V, cordycepin content
The amount (S) of s=cordycepins/mycelia weight (M).(note：Unit conversion should be carried out by bringing into when data calculate)
Embodiment 6
1) extraction will be mixed with 15 times of volume distilled water after 50 parts of trollflower drying and crushings, obtains trollflower Aqueous extracts；
2) basal medium is prepared according to following parts by weight of component：200 parts of potato, 20 parts of wheat bran, 20 parts of niblet, grape
1000 parts of 25 parts of sugar, 8 parts of peptone, 3 parts of microelement composition and distilled water；Wherein, the microelement composition by
The component of following parts by weight is constituted：Potassium dihydrogen phosphate 0.6, ferrous sulfate 0.2, magnesium sulfate 0.5, zinc sulfate 0.4 and calcium carbonate
0.9 and vitamin B10.0015.Gained trollflower water is added into basal medium according to the ratio of mass percent 7% to carry
Liquid obtains pharmacological property culture medium.
3) 100 milliliters of dosing culture medium in each triangular flask, sealed membrane sealing, labelled high pressure steam sterilization 121
DEG C, 20 minutes.Every bottle of inoculation cordyceps militaris link bacterial strain 7% of superclean bench is moved into after cooling, temperature gradient cultivation is cultivated, always
Fermentation time 9 days, specially：First stage cultivates 3 days, and it is Celsius that cultivation temperature is incremented to 20 from first day from 18 degrees Celsius
Degree is incremented by 1 degree Celsius in every 24 hours；Second stage culture 2 days, cultivation temperature are 22 degrees Celsius；Phase III cultivates 4 days, training
It is 28 degrees Celsius to support temperature.Cordyceps militaris zymotic fluid and cordyceps mycelium are respectively obtained after fermentation.Pass through meter
It calculates, mycelium content is 2.02g/ml.
4) cordycepin content assay method such as embodiment 1, it is 14.34mg/g to measure cordycepin content in mycelium.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (10)
1. a kind of preparation method of Cordyceps militaris chiffon cake, wherein include the following steps：
Step 1, the component for weighing following parts by weight：Egg 40-60, flour 15-20, fruiting bodies of cordyceps militaris powder 2-3, cordyceps
Liquid 10-12, edible oil 7-12, sugar 10-30 and lemon juice 1-5；
Step 2 mixes after dismissing egg with remaining ingredient, obtains cake paste；
Step 3 toasts the sizing of gained cake paste to get the Cordyceps militaris chiffon cake.
2. the preparation method of Cordyceps militaris chiffon cake as described in claim 1, wherein step 1 includes following parts by weight
Component：Egg 45-55, flour 17.6, fruiting bodies of cordyceps militaris powder 2.4, Cordyceps militaris bacterium solution 11, edible oil 9, sugar 15-20 and lemon
Juice 1-5.
3. the preparation method of Cordyceps militaris chiffon cake as described in claim 1, wherein the specific preparation of cake paste in step 2
Method is：Egg is divided into egg white and yolk, gained egg white is beaten and is foamed, divide in whipping process 2-4 addition lemon juice and
Sugar obtains protein paste；Yolk, flour, fruiting bodies of cordyceps militaris powder, Cordyceps militaris bacterium solution, edible oil are mixed and turned, yolk is obtained
Paste, gained protein paste is mixed with egg yolk paste to get the cake paste.
4. the preparation method of Cordyceps militaris chiffon cake as described in claim 1, wherein the Cordyceps militaris daughter bacteria liquid is by lily feet
Anther culture medium inoculated cordyceps militaris link bacterial strain ferments to obtain, wherein the trollflower pharmacological property culture medium in basal medium by adding
The trollflower Aqueous extracts for entering mass percent 3-8% obtain.
5. the preparation method of Cordyceps militaris chiffon cake as described in claim 1, wherein the basal medium includes following heavy
Measure the component of part：150-250 parts of potato, 15-25 parts of wheat bran, 10-20 parts of niblet, 15-25 parts of glucose, 3-8 parts of peptone
And 800-1200 parts of distilled water；The trollflower Aqueous extracts additional proportion is the 7% of basic culture medium quality.
6. the preparation method of Cordyceps militaris chiffon cake as claimed in claim 4, wherein the trollflower Aqueous extracts are by trollflower
It mixes extraction with distilled water to obtain, extracting method is temperature gradient extraction method, specifically includes following steps：
Step 1.1 mixes the trollflower after drying and crushing with the distilled water of 5-7 times of volume, and temperature is kept for 42-45 degrees Celsius,
Extraction time is 2-3 hours, is separated by filtration and respectively obtains the first extracting solution and first dregs of a decoction；
Step 1.2 mixes first dregs of a decoction with the distilled water of 3-5 times of volume, and temperature is kept for 60-65 degrees Celsius, and extraction time is
It 4-6 hours, is separated by filtration to obtain the second extracting solution and second dregs of a decoction；
Step 1.3 mixes second dregs of a decoction with 2-3 times of volume distilled water, boils 10-15 minutes, is separated by filtration to obtain third and carries
Take liquid and the third dregs of a decoction；
Step 1.4 mixes the first extracting solution, the second extracting solution and third extracting solution to get the trollflower Aqueous extracts.
7. the preparation method of Cordyceps militaris chiffon cake as described in claim 1, wherein the fruiting bodies of cordyceps militaris powder is by pupa worm
Grass seed entity drying and crushing obtains.
8. the preparation method of the Cordyceps militaris chiffon cake as described in claim 7 or 6, wherein the fruiting bodies of cordyceps militaris is by pupa
Cordyceps sinensis solid state cultivation medium culture obtains, wherein the Cordyceps militaris solid state cultivation culture medium includes the component of following parts by weight：
Cooked rice 40-60, wheat bran 15-25, dried silkworm chrysalis meal 8-12, trollflower dregs of a decoction 5-10, sawdust 5-10, magnesium sulfate 0.05-0.15, wheat
Powder 5-7, glucose 1-3 and water 100-150, pH6-7.
9. the preparation method of Cordyceps militaris chiffon cake as claimed in claim 8, wherein the Cordyceps militaris solid state cultivation culture medium
Include the component of following parts by weight：It is cooked rice 50, wheat bran 20, dried silkworm chrysalis meal 10, the trollflower dregs of a decoction 6, sawdust 6, magnesium sulfate 0.1, small
Flour 6, glucose 2 and water 120, pH6-7.
10. the preparation method of Cordyceps militaris chiffon cake as claimed in claim 8 or 9, wherein the culture side of fruiting bodies of cordyceps militaris
Method is：The Cordyceps militaris solid state cultivation culture medium, which is bottled to 1/2, expires, sealing, and 0.15MPa high pressure sterilization 2h are inoculated with Cordyceps militaris
25 DEG C of dark situation cultures after mycelium, mycelia, which is covered with, moves to bright place, 15 DEG C -25 DEG C continue culture 25-40 days it is orange red to having
Stroma is formed and is grown to 30-66mm high to get the fruiting bodies of cordyceps militaris.
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