CN108635493A - Antitumor and cirsoid pharmaceutical composition for the treatment of and its preparation method and application - Google Patents
Antitumor and cirsoid pharmaceutical composition for the treatment of and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to a kind of antitumor and cirsoid pharmaceutical compositions for the treatment of and its preparation method and application, belong to tcm field.The pharmaceutical composition is made of the raw material of following parts by weight:10 50 parts of nine bursts of longan, 10 100 parts of ox, 10 50 parts of dandelion, 5 50 parts of Rhizoma Chuanxiong, 10 50 parts of bush, 10 50 parts of Radix Astragali, 10 50 parts of paris polyphylla and lopseed.Pharmaceutical composition provided by the invention has righting reinforcing and immune function facilitation, can reduce tumor patient pain, extend life cycle, and through long-term experiment studies have shown that effect of the present invention is better than other antitumor medicine compositions.Brain tumor is tested and clinical test proves that the pharmaceutical composition of the present invention has excellent therapeutic effect to rat pituitary tumor model, curative for effect reliable.The present invention is safe, has no toxic side effect.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition, especially a kind of cirsoid pharmaceutical composition of antineoplaston, also
Relate to and its preparation method and application, belong to tcm field.
Background technology
The cancer of early stage generally selects operative treatment, but postoperative the problem of still suffering from recurrence and transfer.And middle and advanced stage
The primary treatments of cancer are still based on chemotherapy at present.
Breast cancer is one of the most common malignant tumors in women, and according to statistics, incidence accounts for the various malignant tumours of whole body
7-10%, be only second to uterine cancer in women, its morbidity it is often related with heredity and 40-60 Sui between, before and after menopause
Women's incidence is higher.Usually it is happened at the malignant tumour of breast galandular epithelium tissue.It is that one kind seriously affecting women's physical and mental health
Even one of the most common malignant tumour of threat to life.
In recent years, intracranial tumors incidence is in rising trend, and according to statistics, intracranial tumors account for about the 5% of general tumour, account for
The 70% of virgin tumour, and other malignant tumours finally have 20-30% and are transferred to encephalic, due to the infiltrative growth of its expansion,
Encephalic is when according to certain space, no matter its property is benign or pernicious, certainly will all intracranial pressure be made to increase, and oppresses brain tissue,
Cause nervous centralis to damage, jeopardizes patient vitals.
The treatment of Pituitary adenoma includes mainly three kinds of operation, drug and radiotherapy therapies.Just because of no one
Kind method can achieve the purpose that cure each hypophysoma completely, so various therapies cut both ways.About radiotherapy, by
Belong to adenoma in hypophysoma, itself is poor to the sensibility of radiotherapy, and nearly pituitary function occur low by the patient of 70-80% after radiotherapy
Subtract, reduce the quality of life of patient, operation is remaining, is not resistant to operation, is insensitive to drug, having so radiotherapy is only applicable to
Suffering from the disease altogether can not undergo surgery or the patient of drug therapy.
The drug for clinically treating all kinds of tumours at present mainly has chemicals, but since chemotherapeutics toxicity is big, is killing
Also human autoimmunes' cell such as kill leucocyte, lymphocyte of non-selectivity while dead tumour cell, causes patient normal
Often due to being complicated by infection because of autoimmunity inferior capabilities, patient uses erious adverse reaction, and the course for the treatment of is long, costly, makes in clinic
There is larger limitation in.
Traditional Chinese medicine has unique advantage in terms for the treatment of tumour, has poison is secondary to make by the natural class drug of representative of Chinese medicine
With the features such as small, action target spot is various, tolerance is good, more and more it is taken seriously in terms for the treatment of tumour.Chinese medicine thinks, tumour
Formation is that for rule, disorderly, channels and collaterals are obstructed qi and blood fortune, and qi and blood pent-up is formed in a certain position, and corporal parts will appear.
But the anti-breast cancer Chinese medicine compound prescription dosage reported at present is larger, impurity component is more, curative effect unobvious.
Varication refers to due to factors such as hypostasis, vein tube wall weaknesses, and caused vein is tortuous, expands.Body
Varicose can occur for the vein at multiple positions, for example hemorrhoid are exactly a kind of varication in fact, clinical visible also gastric and esophageal
Bottom varication, varicocele and subcutaneous varicos vein of abdominal wall etc..The position that varication occurs most frequently is in lower limb.It is worth strong
It adjusts, varication itself may be the secondary manifestation of other lesions, such as vena cave occlusion, answer positive treatment protopathy.
Treatment varication mostly uses operative treatment at present, that is, makees venous stripping.If operation cannot be by the quiet of varicose
Arteries and veins all strips off, and the intravenous of the varicose left can make wall of vein generate aseptic inflammation with injection of sclerosing agent, mutually viscous to wall
It, no longer varicose.Through clinical practice for many years, can also be recurred after many patient's operations, uncertain therapeutic efficacy is cut, and Repeated Operation also fails to control
More.Modus operandi not only has patient certain pain, but also costly, and cure rate is very low.
Chinese medicine thinks that varication is because of native endowment deficiency, and tendon and vessel is weak, and long row is stood long in addition, overworked, into one
Step damage tendon and vessel, so that passages through which vital energy circulates does not conform to, QI-blood circulation is unsmooth, and blood is stopped up under, and the expansion of congestion blood block train of thought is full, with the passing of time interleaved discs
Qu Ercheng.With the passing of time similar to the shape of knurl.Also have because after long journey, fatigue, paddling and drenching with rain, by cold-dampness, Han Ningxuemai, stasis muscle
Train of thought road and form varication.The advantage that traditional Chinese medicine is played a role with its multipath, too many levels, multiple target point is widely used in each
Kind disease treatment.It was mostly controlled in the past with heat-clearing and damp-drying drug for cirsoid Chinese traditional treatment, and ignored cool blood, promoting blood circulation, detoxifies it
It treats, therefore therapeutic effect is poor.
In addition, existing Chinese medicine also tends to be to have the effect of single, therefore, it is good to research and develop a kind of antitumous effect,
Dosage is few, and has no toxic side effect, while can treat cirsoid pharmaceutical composition and be of great significance.
Invention content
In order to solve the above technical problem, the present invention provides a kind of antitumor and cirsoid pharmaceutical composition for the treatment of and
Preparation method and application, the Chinese medicine can not only anti-cancer and detumescence, also have effects that treat varication.The specific side of the present invention
Case is as follows:
A kind of antitumor and cirsoid pharmaceutical composition for the treatment of, is made of the raw material of following parts by weight:
10-100 parts of ox of nine bursts of longan, 10-50 parts of dandelion, 5-50 parts of Rhizoma Chuanxiong, 10-50 parts of bush, 10-50 parts of Radix Astragali, Yunnan weight
Building 10-50 parts and 10-50 parts of lopseed.
Further, it is made of the raw material of following parts by weight:Nine bursts of longan, 50 parts of ox, 25 parts of dandelion, 20 parts of Rhizoma Chuanxiong,
30 parts of 30 parts of bush, 30 parts of Radix Astragali, 40 parts of paris polyphylla and lopseed.
The preparation method of pharmaceutical composition of the present invention, includes the following steps:Raw material is weighed in parts by weight, is mixed
Uniformly, it water cover is added crosses medicinal material surface and impregnate 5-30 minutes, boiled 5-30 minutes to get to described pharmaceutical composition.
Further, dosage is daily 2-3 times, each 100ml-500ml.
Further, described pharmaceutical composition is combined with pharmaceutically acceptable carrier be prepared into oral solution, granule,
The drug of capsule or Tabules.
Application of the pharmaceutical composition of the present invention in preparing tumor.
Chinese medicine composition provided by the invention screens prescription, Fang Zhong according to traditional Chinese medical theory dialectical treatmert:
Nine bursts of oxen of longan, property is sweet, bitter, warm, synthetism myogenic, stasis-dispelling and pain-killing.Fracture is controlled, traumatic injury, rheumatic arthritis, menstruation is not
It adjusts, carbuncle furuncle poison is swollen, scrofula.
Dandelion, bitter sweet are trembled with fear;Enter liver, stomach;It is clearing heat and detoxicating, diuresis dissipating bind.Control acute mastitis, adenolymphitis, scrofula
Scrofula, boil poisonous soreness, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urine
Road feel contaminates.
Rhizoma Chuanxiong, it is acrid in flavour and warm in nature;Promote breath and open depression, method wind-dryness is wet, promoting blood circulation and stopping pain.It is dizzy to control air-cooled headache rotation, hypochondriac pain abdomen pain, arthritis muscle
Contraction, Amenorrhea, difficult labour, postpartum stasis block pain, ulcer sores.For irregular menstruation, closed dysmenorrhea, lump in the abdomen abdominal pain, chest side of body shouting pain falls and flutters
Swelling and pain, headache, arthralgia pain due to rheumatism.
Bush, it is sweet;It is salty;Xin Liang;The thoughts of returning home, liver, stomach, the spleen channel;Promoting blood circulation, detumescence ding-tong, such as married woman's amenorrhea due to stagnation of blood, pain
Through, postpartum stasis pain in the chest and abdomen, postpartum anemic fainting, carbuncle swells, traumatic injury, lockjaw.
Radix Astragali, sweet, tepor;Return lung, spleen, liver and kidney channel;
Paris polyphylla has the effect of clearing heat and detoxicating, swelling and pain relieving, cool liver arresting convulsion, for carbuncle swells, abscess of throat, venomous snake bite, bruise
The diseases such as the pain of injury, convulsion.
Lopseed, sweet, acrid, warm;Enter lung, liver two passes through;Cure mainly dispelling wind and eliminating dampness, the product pungent-warm, it is pungent can manage it is scattered, temperature overcome the coldness it is wet,
Relaxing tendons and activating collaterals, promoting blood circulation and stopping pain, solution poison rash.
Wherein, the cooperation of nine bursts of oxen, dandelion, Radix Astragali, Rhizoma Chuanxiong, bushes of longan according to the above ratio is antitumor, especially right
Brain tumor, breast cancer have notable curative effect, have certain curative effect to kidney failure.
The cooperation of longan nine bursts of oxen, Radix Astragali, paris polyphylla, lopseeds according to the above ratio has certain curative effect to varication.
Compared with prior art, beneficial effects of the present invention are as follows:
(1)Pharmaceutical composition provided by the invention has righting reinforcing and immune function facilitation, can promote tumor necrosis factor
The generation of sub (INF) plays multiple target point, and multiple organ treats the effect of tumour, can reduce tumor patient pain, extend life cycle,
And through long-term experiment studies have shown that effect of the present invention is better than other antitumor medicine compositions.
(2)Brain tumor is tested and clinical test proves pharmaceutical composition of the invention to rat pituitary tumor model with excellent
Therapeutic effect, it is curative for effect reliable.
(3)The present invention is safe, has no toxic side effect.
Specific implementation mode
It is clearly and completely described below in conjunction with the technical solution in embodiment, it is clear that described embodiment is only
Only it is rather than whole example to a part of example of the present invention.Based on the embodiments of the present invention, ordinary skill people
The every other embodiment that member is obtained under the premise of not making the creative labor, shall fall within the protection scope of the present invention.
Embodiment 1
The treatment tumour of the present embodiment treats mastadenoma, cirsoid pharmaceutical composition is treated, by the original of following parts by weight
Material is made:
Nine bursts of longan, 50 parts of ox, 25 parts of dandelion, 20 parts of Rhizoma Chuanxiong, 30 parts of bush, 30 parts of Radix Astragali, 40 parts of paris polyphylla and lopseed 30
Part.
Weigh raw material in parts by weight, be uniformly mixed, be added water cover cross medicinal material surface impregnate 10 minutes, boiled 8 minutes, i.e.,
Obtain described pharmaceutical composition.
Embodiment 2
The antitumor and cirsoid pharmaceutical composition for the treatment of of the present embodiment, is made of the raw material of following parts by weight:
Nine bursts of longan, 20 parts of ox, 10 parts of dandelion, 42 parts of Rhizoma Chuanxiong, 40 parts of bush, 35 parts of Radix Astragali, 15 parts of paris polyphylla and lopseed 20
Part.
Weigh raw material in parts by weight, be uniformly mixed, be added water cover cross medicinal material surface impregnate 20 minutes, boiled 15 minutes,
Obtain described pharmaceutical composition.Diluent lactose is added in aforementioned pharmaceutical compositions or cornstarch is uniformly mixed, whole grain,
It is dry, granule is made.
Embodiment 3
The antitumor and cirsoid pharmaceutical composition for the treatment of of the present embodiment, is made of the raw material of following parts by weight:
Nine bursts of longan, 90 parts of ox, 20 parts of dandelion, 20 parts of Rhizoma Chuanxiong, 20 parts of bush, 15 parts of Radix Astragali, 20 parts of paris polyphylla and lopseed 40
Part.
Weigh raw material in parts by weight, be uniformly mixed, be added water cover cross medicinal material surface impregnate 10 minutes, boiled 20 minutes,
Obtain described pharmaceutical composition.Aforementioned pharmaceutical compositions are processed into pulvis by existing method.
Embodiment 4
The antitumor and cirsoid pharmaceutical composition for the treatment of of the present embodiment, is made of the raw material of following parts by weight:
Nine bursts of longan, 70 parts of ox, 35 parts of dandelion, 30 parts of Rhizoma Chuanxiong, 20 parts of bush, 30 parts of Radix Astragali, 30 parts of paris polyphylla and lopseed 25
Part.
Weigh raw material in parts by weight, be uniformly mixed, be added water cover cross medicinal material surface impregnate 30 minutes, boiled 15 minutes,
Obtain described pharmaceutical composition.Aforementioned pharmaceutical compositions are processed into capsule by existing method.
5 Clinic Case of embodiment
45 years old male's brain tumor patients with terminal, take the drug of embodiment 1,2-3 times daily, each 500ml is good after taking 3 months
Turn, does not recur.
50 years old Female breast cancer patients, take the drug of embodiment 1,2-3 times daily, each 200ml, after taking two weeks
It improves, does not recur.
70 years old male's varicose vein patients, take the drug of embodiment 3,2-3 times daily, each 250g baths, 3 months
Left and right recovery from illness, does not recur.
55 years old male's varicose vein patients, take the drug of embodiment 4,2-3 times daily, 3 tablets each time, fully recover from an illness within 3 months or so
More, it does not recur.
6 anti-breast cancer pharmacological evaluation of embodiment
(1), to the inhibiting effect of human breast carcinoma in nude mouse (MCF-7) cell
Experimental method:MCF-7 Human Breast Cancer Cells suspension is prepared, cell concentration is adjusted, armpit inoculates human milk gland before every mouse right side
Cancer MC F-7 cells 5 × 106A/0 .2ml is inoculated with 20 altogether, is randomly divided into treatment group's (having of providing of the embodiment of the present invention 1
The Traditional Chinese medicine compound composition of anti-breast cancer activity, 001. gastric infusion of lot number, 2g/kg) and blank group (waiting capacity physiological saline),
It after every group 10,24 hours, starts medication, once a day, continuous 24 days, after drug withdrawal, puts to death animal, observe tumour growth situation
And take tumor tissue and weigh, specific experiment the results are shown in Table 1.
Inhibiting effect of the table 1 to human breast carcinoma in nude mouse (MCF-7) cell
(2), the test of external anti-breast cancer activity
Cell strain:Human breast carcinoma (MCF-7) cell;96 orifice plates are purchased from Costar companies;RMPI1640 culture mediums are public purchased from Gibco
Department;DMEM culture mediums are purchased from Gibco companies;Newborn bovine serum is purchased from Hangzhou Chinese holly company;Tetrazolium bromide (MTT) and DMSO are purchased from
Amresco companies.
Used laboratory apparatus is as follows:
Laboratory apparatus:Superclean bench (Suzhou purifies model SW-CJ-IFD), CO2 incubators (SANYO models:XD-101),
Microplate reader BIO-RAD (Model NO .550Serial NO.16971).
Used experimental method is as follows:
Experimental method:External anti-human mammary gland is carried out to the Chinese medicine composition that the embodiment of the present invention 1 is prepared using improvement mtt assay
Cancer cell is tested:Human breast carcinoma (MCF-7) is digested with pancreatin, counts, be made a concentration of 5 × 104The cell of a/ml is outstanding
Liquid.100 μ l cell suspensions will be added in 96 orifice plates per hole (per hole 5 × 103A cell), 37 DEG C are subsequently placed in, 5%CO2Incubator
Middle culture 24 hours;Drug is diluted to required different gradient concentrations with non-fully culture medium, and 100 μ L are added per hole and contain accordingly
Medicine culture medium, while negative control group is set up, vehicle control group and positive controls, then 96 orifice plates are placed in 37 DEG C, 5%CO2
It is cultivated 72 hours in incubator.Then 20 μ L MTT (5mg/ml) are added per hole, continue culture 4 hours, terminates culture, discards training
Base is supported, is added 150 μ L DMSO dissolvings per hole, the gently mixing of shaking table 10 minutes.Enzyme is used under λ=4570nm, two wavelength of 620nm
Absorbance, that is, OD value of the mark instrument detection per hole calculates the suppression of each drug using the average value of each multiple holes as the OD values of this group of cell
Rate processed.
Experimental result shows the Chinese medicine composition that is prepared of the embodiment of the present invention 1, to human breast carcinoma (MCF-7) have compared with
Strong inhibiting effect, each dosage group present preferable dose-effect relationship.Experimental result is as shown in table 2.
Inhibiting effect result of the table 2 to human breast carcinoma (MCF-7)
By above the experimental results showed that:Relative to conventional pharmaceutical composition(Inhibiting rate is in 50%-60%), tool provided by the invention
There is the Chinese medicine composition of anti-human mammary gland cancer activity to be significantly inhibited to being transplanted in human breast carcinoma nude mouse, also has in vitro
Play the role of that human breast cancer cell is obviously inhibited to grow.Show the Chinese traditional medicine composition provided by the invention with anti-human mammary gland cancer activity
Object has the function of significant anti-human breast cancer, can be used for the treatment of breast cancer disease.
Embodiment 7 inhibits brain tumor experiment
Prepare Wistar rats 120, be randomly divided into blank group, control group and embodiment 1-10 groups, often gives 10.Except sky
White group is outer, is all made of estrogen method and prepares Pituitary adenoma model.Meanwhile blank group and control group give physiological saline, and implement
Example 1-4 groups give the pharmaceutical preparation prepared in 1-4 of the embodiment of the present invention respectively.Rat is put to death after two weeks, takes out hypophysis, weighs weight
Amount.
The result shows that the pituitary average weight of control rats is 3.6 times of blank group, show modeling success;Implement
Average weight is respectively less than 2.8 times of blank group in the group of example 1-4 group rat pituitaries.There is significant difference between group.Illustrate this
Invention preparation has excellent therapeutic effect to rat pituitary tumor model.
8 toxicity of embodiment is verified
50 healthy mices, weight 80-120g are selected to be randomly divided into 5 groups, every group of half male and half female, respectively with the medicine of embodiment 1-4
Preparation made of compositions is administered, and ejection preparation per injection amount is 0.2mL/10g, oral preparation gastric infusion
0.4ml/10g, once in the morning and once at night.After administration 7 days, the mouse of embodiment 1-4 there are no any Novel presentation, and diet is movable good
Good, none is dead.Show within the scope of the prescription of the present invention, drug safety.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (6)
1. a kind of antitumor and cirsoid pharmaceutical composition for the treatment of, it is characterised in that:By the raw material system of following parts by weight
At:
10-100 parts of ox of nine bursts of longan, 10-50 parts of dandelion, 5-50 parts of Rhizoma Chuanxiong, 10-50 parts of bush, 10-50 parts of Radix Astragali, Yunnan weight
Building 10-50 parts and 10-50 parts of lopseed.
2. pharmaceutical composition according to claim 1, it is characterised in that:It is made of the raw material of following parts by weight:Longan
30 parts of nine bursts of 50 parts of oxen, 25 parts of dandelion, 20 parts of Rhizoma Chuanxiong, 30 parts of bush, 30 parts of Radix Astragali, 40 parts of paris polyphylla and lopseed.
3. the preparation method of pharmaceutical composition as claimed in claim 1 or 2, it is characterised in that:Include the following steps:By weight
Number weighs raw material, is uniformly mixed, and water cover is added crosses medicinal material surface and impregnate 5-30 minutes, boiled 5-30 minutes to get to described
Pharmaceutical composition.
4. the preparation method of pharmaceutical composition according to claim 3, it is characterised in that:Dosage is to take 2-3 daily
It is secondary, each 100ml-500ml.
5. the preparation method of the pharmaceutical composition described in claim 3, it is characterised in that:By described pharmaceutical composition and pharmaceutically
Acceptable carrier combination is prepared into the drug of oral solution, granule, capsule or Tabules.
6. application of the pharmaceutical composition in preparing antitumor or treatment varication drug described in one of claim 1-3.
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