CN108633910A - The sterilized pharmaceutics of graphene oxide and Antagonistic Fungi streptomycete compounding - Google Patents
The sterilized pharmaceutics of graphene oxide and Antagonistic Fungi streptomycete compounding Download PDFInfo
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- CN108633910A CN108633910A CN201810511641.1A CN201810511641A CN108633910A CN 108633910 A CN108633910 A CN 108633910A CN 201810511641 A CN201810511641 A CN 201810511641A CN 108633910 A CN108633910 A CN 108633910A
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- China
- Prior art keywords
- phytophthora
- graphene oxide
- plant
- streptomyces griseoflavus
- germ
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
The invention discloses the sterilized pharmaceutics that graphene oxide and Antagonistic Fungi streptomycete compound.The present invention researches show that, graphene oxide has significant inhibiting effect to a variety of phytophthora germs, especially apparent synergistic function can be played with the combination of Antagonistic Fungi Streptomyces Griseoflavus, it is notable to plant phytophthora root rot preventive effect, the biological disease-preventing preparation developed controlling plant diseases, improve crop yield is can be applied to, there is good popularizing application prospect in relevant agricultural production.
Description
Technical field
The invention belongs to agricultural biological technical fields.It is compounded more particularly, to graphene oxide and Antagonistic Fungi streptomycete
Sterilized pharmaceutics.
Background technology
Phytophthora (Phytophthora) is mostly parasitic germ, endangers the crushing pathogen of economic plants, and induction is permitted
More important plant disease, caused disease are commonly referred to as epidemic disease.Prevent currently, the prevention of plant phytophthora root rot mostly uses physics
It controls, Agro-chemicals control and Antagonistic Fungi biological control means.But there are various defects for existing method, such as physical control mode
Preventive effect is poor, and chemical agent pollution is big, is also easy to produce drug resistance, and Antagonistic Fungi preventive effect is unstable and with selectivity.
Graphene oxide (GO) is the oxide of graphene, and researches show that with potential antibacterial applications foreground.Hu etc. exists
The antibacterial action for reporting GO for the first time in 2010, this experimental result cause the extensive of numerous researchers immediately once delivering
Concern.Then, the experimental results show GO to a variety of common bacterias, such as escherichia coli, staphylococcus aureus and typhoid fever
Detection of Salmonella etc. all shows excellent antibacterial activity.However, GO as a kind of antiseptic with potential using value also by
Query.Ruiz etc. has found that GO is not only unable to antibacterial, but also can be used as the skeleton of bacterial adhesion, promotes growth, proliferation and shape
At bacterial biof iotalm.In addition, not yet illustrating completely the research of the antibacterial mechanisms of GO so far.
In short, according to many studies have shown thats although the anti-microbial property of GO has dispute, still have potential anti-
Bacterium foreground, only its antibacterial activity is with apparent selectivity.
Invention content
The purpose of the present invention is overcoming the defect and deficiency of existing plant phytophthora root rot Prevention Technique, provide graphene oxide and
Streptomyces Griseoflavus is combined the application in terms of preventing plant phytophthora germ.
Another object of the present invention is to provide a kind of novel plant phytophthora germ antiseptic.
Above-mentioned purpose of the present invention is achieved through the following technical solutions：
The present invention is the study found that graphene oxide has significant suppression to phytophthora root rots such as Phytophthora nicotianae Breda, phytophthora blight of pepper
It makes and uses, especially apparent synergistic function can be played with the combination of Antagonistic Fungi Streptomyces Griseoflavus, to plant phytophthora root rot preventive effect
Significantly, the biological disease-preventing preparation developed controlling plant diseases, improve crop yield is can be applied to, is had in relevant agricultural production
There is good popularizing application prospect.
Therefore, applying below should all be within protection scope of the present invention：
Application of the graphene oxide in terms of improving plant phytophthora germ Antagonistic Fungi Streptomyces Griseoflavus preventive effect.
Graphene oxide and Streptomyces Griseoflavus are combined the application in terms of preventing plant phytophthora germ.
Graphene oxide and Streptomyces Griseoflavus are combined the application in terms of preparing plant phytophthora germ antiseptic.
Wherein preferably, the plant phytophthora germ is peronophythora litchi (Peronophthora litchii
Chen), Phytophthora nicotianae Breda (Phytophthora nicotianae), soyabean phytophthora (phytophthora sojae), cucumber
Phytophthora (Phytophthora melonis) or phytophthora blight of pepper (Phytophthora capsici Leonian).
In addition, the antiseptic of the crop phytophthora germ containing graphene oxide and Streptomyces Griseoflavus, and contain oxidation stone
The medicament of the prevention crop phytophthora root rot of black alkene and Streptomyces Griseoflavus, also all should be also within the scope of the present invention.
The bacteriostasis of studies have shown that graphene oxide has dose dependent.
Preferably, in the antiseptic, the concentration of graphene oxide is not less than 3g/L.
It is highly preferred that in the antiseptic, a concentration of 3~20g/L of graphene oxide.
It is highly preferred that in the antiseptic, a concentration of 5~15g/L of graphene oxide.
Preferably, the mass ratio of Streptomyces Griseoflavus and graphene oxide is 100：4~9.
The present invention is explored by numerous studies, it is found that graphene oxide has selectivity to the inhibiting effect of germ, to certain
The growth of a little specific biological cells is inhibited, and has to the growth ability of other biological cells and promote to improve
Effect.It is tested based on a large amount of screening study, the present invention proposes that graphene oxide has excellent inhibition to a variety of phytophthora germs
Activity, and can be combined with Streptomyces Griseoflavus and show excellent synergistic function, it is obviously improved and plant phytophthora root rot is prevented
Effect, mechanism therein still need to further study, thus it is speculated that and may be the growth that graphene oxide can promote these bacteriums, therefore,
While playing itself inhibition phytophthora germ effect, additionally it is possible to which significant collaboration is played in the effect for promoting cause of disease Antagonistic Fungi
Synergistic effect.
The invention has the advantages that：
Studies have shown that graphene oxide of the present invention to a variety of phytophthora germs have significant inhibiting effect, especially with it is short of money
The combination of antibacterial Streptomyces Griseoflavus can play apparent synergistic function, notable to plant phytophthora root rot preventive effect, can be applied to develop
Controlling plant diseases, the biological disease-preventing preparation for improving crop yield have in relevant agricultural production and promote and apply well
Foreground.
Specific implementation mode
Further illustrated the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagent, methods and apparatus.
Unless stated otherwise, agents useful for same and material of the present invention are purchased in market.
The anti-phytophthora activity of 1 graphene oxide of embodiment
1, experiment material
(1) for trying pathogen：Soyabean phytophthora (phytophthora sojae), Phytophthora nicotianae Breda (Phytophthora
Nicotianae), peronophythora litchi (Peronophthora litchii Chen), cucumber phytophthora (Phytophthora
Melonis), phytophthora blight of pepper (Phytophthora capsici Leonian).
(2) medicament：Gained graphene oxide (GO) is prepared using conventional hummers methods.
2, live body pathogen mycelial growth rate method
(1) it adds graphene oxide into sterilized and cooling Pathogen culture base, mixing is diluted to containing for series concentration
Medicine culture medium (0.1,1,3,5,10,15,20,25g/L), per culture dish be inoculated with a bacterium dish (diameter d=0.6cm), each concentration
If 3 repetitions；Culture medium to be not added with graphene oxide is control (CK), using metalaxyl as positive control medicament, measures oxidation
Graphene is to the inhibitory activity for trying pathogen.It is handed over cross when at 25 DEG C, culture covers with 2/3 or more culture dish to control bacterium colony
Fork method measures the colony diameter (cm) of each concentration processing, its average value is taken to represent, and calculates medicament to mycelial growth inhibition ratio.
(2) the results are shown in Table 1.
The inhibiting effect of 1 five kinds of various pathogenic bacterias of graphene oxide pair of table
As seen from the results in Table 1, graphene oxide processing shows the mycelia growth of five kinds of various pathogenic bacterias excellent
Inhibitory activity, and there is dose dependent, preferred concentration is 3~20g/L, most preferably 5~15g/L.
3, dilution plate counting method carries out the measurement of micro organism quantity
(1) mixing in sterilized and cooling Pathogen culture base is added graphene oxide into, containing for series concentration is diluted to
Medicine culture medium (0.1,1,3,5,10,15,20,25g/L), per culture bottle inoculation pathogen cultivated, each concentration sets 3 weights
It is multiple；It is control to be not added with graphene oxide (CK), using metalaxyl as positive control medicament.Processing is carried out in aseptic operating platform, examination
Container used prevents living contaminants through high-temperature sterilization in operating process in testing.Constant temperature incubation starts counting up after 3 days, continuous to count
Number three days, it is once final result to take bacterium number at most, as a result takes the average value of 3 repetitions.
(2) the results are shown in Table 2.
The inhibiting effect of 2 five kinds of various pathogenic bacterias of graphene oxide pair of table
As seen from the results in Table 2, compared with the control group, graphene oxide processing shows five kinds of various pathogenic bacterias excellent
Growth inhibitory activity, and have dose dependent.
The potted plant experiment of the anti-phytophthora of 2 graphene oxide of embodiment
1, experiment material is with embodiment 1, while by graphene oxide and water mixing, be made into a series of concentration (1,3,5,7,
9,11,13,15,17,19,21g/L) antimicrobial fluid.Then soybean, tobacco, cucumber, Hot Pepper Seedling are utilized respectively and carries out potting reality
It tests.
2, experimental method
Graphene oxide antimicrobial fluid is uniformly poured in the plantling root that cotyledon is sufficiently spread out, is inoculated with each cause of disease afterwards for 24 hours
Bacterium pours primary, continuous pouring 3 times for every 7 days, is control with clear water and blank cultures, and each processing is repeated 3 times, after processing
It is measured by sampling within 21 days, calculates preventive effect.
3, the results are shown in Table 3 for diseases prevention.
3 graphene oxide of table prevents the measurement result of plant phytophthora root rot
The results show that the different phytophthora root rots of five kinds of graphene oxide pair show good preventive effect, to peronophythora litchi,
Phytophthora nicotianae disease, the preventive effect of phytophthora sojae kaufmann＆gerdemann are best, and have dose dependent.
3 graphene oxide of embodiment and anti-phytophthora activity associated with Streptomyces Griseoflavus
1, experiment material
(1) pathogen：With embodiment 1.
(2) antibacterial components：
Graphene oxide (GO) and Antagonistic Fungi：Streptomyces Griseoflavus (Streptomycesgriseoflavus) (it is purchased in market,
Viable count about 1.00 × 109/ g), trichoderma asperellum (Trichoderma asperellum) (purchased in market, viable count about 1.00 × 109/
g)。
2, experimental method
Respectively by Antagonistic Fungi and graphene oxide according to 100：5 quality is diluted with water to antimicrobial fluid, wherein oxygen than mixing
The final concentration of 10g/L of graphite alkene.
In addition with 10g/L graphene oxides antimicrobial fluid and metalaxyl it is respectively control.
Referring next to the method for embodiment 1, live body pathogen mycelial growth rate method test bacteriostasis rate and dilute is respectively adopted
Release the measurement that colony counting method carries out micro organism quantity.
3, result is as shown in table 4 and 5
The inhibiting effect of 4 five kinds of various pathogenic bacterias of each group pair of table
The inhibiting effect of 5 five kinds of various pathogenic bacterias of each group pair of table
By above-mentioned experimental result it is found that graphene oxide is combined with Antagonistic Fungi Streptomyces Griseoflavus, apparent collaboration can be generated
Synergistic effect is significantly improved to the inhibitory activity of five kinds of pathogens.
4 graphene oxide of embodiment and anti-phytophthora potted plant experiment associated with Streptomyces Griseoflavus
1, experiment material is with embodiment 3, and experimental method is with embodiment 2, by antibacterial made of Antagonistic Fungi and graphene oxide
Liquid is utilized respectively soybean, tobacco, cucumber, Hot Pepper Seedling and carries out potted plant experiment.
2, the results are shown in Table 6 for diseases prevention.
6 each group of table prevents the measurement result of plant phytophthora root rot
The results show that graphene oxide is combined with Antagonistic Fungi Streptomyces Griseoflavus, apparent synergistic function can be generated, it is right
The preventive effect of five kinds of plant phytophthora root rots is significantly improved.
The ratio optimization of embodiment 5 graphene oxide and Streptomyces Griseoflavus
Using the amount ratio of graphene oxide and Streptomyces Griseoflavus as variable factors, screening test is optimized.Test material
Material and method are with embodiment 3, and by taking phytophthora blight of pepper as an example, bacteriostasis rate is tested using live body pathogen mycelial growth rate method.Knot
Fruit is as shown in table 7, when the mass ratio of Streptomyces Griseoflavus and graphene oxide is 100：When 4~9, the preventive effect of composite drug is best.
The inhibiting effect of 7 five kinds of various pathogenic bacterias of each group pair of table
Claims (8)
1. application of the graphene oxide in terms of improving plant phytophthora germ Antagonistic Fungi Streptomyces Griseoflavus preventive effect.
2. graphene oxide and Streptomyces Griseoflavus are combined the application in terms of preventing plant phytophthora germ.
3. graphene oxide and Streptomyces Griseoflavus are combined the application in terms of preparing plant phytophthora germ antiseptic.
4. according to any application of claims 1 to 3, which is characterized in that the plant phytophthora germ is Peronophythora Litchii
Bacterium, Phytophthora nicotianae Breda, soyabean phytophthora, cucumber phytophthora or phytophthora blight of pepper.
5. a kind of plant phytophthora germ antiseptic, which is characterized in that contain graphene oxide and Streptomyces Griseoflavus.
6. a kind of medicament of prevention crop phytophthora root rot, which is characterized in that contain graphene oxide and Streptomyces Griseoflavus.
7. medicament described in antiseptic or claim 6 according to claim 5, which is characterized in that Streptomyces Griseoflavus and oxidation
The mass ratio of graphene is 100：4~9.
8. medicament described in antiseptic or claim 6 according to claim 5, which is characterized in that the phytophthora root rot is lichee
White phytophthora, Phytophthora nicotianae, soybean phytophthora, cucumber phytophthora or Phytophthora capsici.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810511641.1A CN108633910A (en) | 2018-05-24 | 2018-05-24 | The sterilized pharmaceutics of graphene oxide and Antagonistic Fungi streptomycete compounding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810511641.1A CN108633910A (en) | 2018-05-24 | 2018-05-24 | The sterilized pharmaceutics of graphene oxide and Antagonistic Fungi streptomycete compounding |
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| Publication Number | Publication Date |
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| CN108633910A true CN108633910A (en) | 2018-10-12 |
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| CN201810511641.1A Withdrawn CN108633910A (en) | 2018-05-24 | 2018-05-24 | The sterilized pharmaceutics of graphene oxide and Antagonistic Fungi streptomycete compounding |
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Application publication date: 20181012 |