CN108925577A - A kind of plant phytophthora root rot antibacterial agent containing graphene oxide - Google Patents

A kind of plant phytophthora root rot antibacterial agent containing graphene oxide Download PDF

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Publication number
CN108925577A
CN108925577A CN201810511644.5A CN201810511644A CN108925577A CN 108925577 A CN108925577 A CN 108925577A CN 201810511644 A CN201810511644 A CN 201810511644A CN 108925577 A CN108925577 A CN 108925577A
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Prior art keywords
phytophthora
graphene oxide
plant
root rot
antibacterial agent
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CN201810511644.5A
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Chinese (zh)
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林荣铨
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林荣铨
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Priority to CN201810511644.5A priority Critical patent/CN108925577A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates

Abstract

The invention discloses a kind of the plant phytophthora root rot antibacterial agent containing graphene oxide, the specifically application of graphene oxide and Antagonistic Fungi atrophy bacillus compounding in terms of preventing and treating plant phytophthora root rot.The present invention researches show that, graphene oxide has significant inhibiting effect to a variety of phytophthora germs, especially apparent synergistic function can be played with the combination of Antagonistic Fungi atrophy bacillus, it is significant to plant phytophthora root rot preventive effect, it can be applied to the biological disease-preventing preparation developed controlling plant diseases, improve crop yield, there is good popularization and application foreground in relevant agricultural production.

Description

A kind of plant phytophthora root rot antibacterial agent containing graphene oxide
Technical field
The invention belongs to agricultural biological technical fields.More particularly, to a kind of plant phytophthora containing graphene oxide Sick antibacterial agent, specifically graphene oxide and Antagonistic Fungi atrophy bacillus compound the application in terms of preventing and treating plant phytophthora root rot.
Background technique
Phytophthora (Phytophthora) is mostly parasitic germ, endangers the crushing pathogen of economic plants, and induction is permitted More important plant disease, caused disease are commonly referred to as epidemic disease.Currently, the prevention and treatment of plant phytophthora root rot mostly uses physics anti- It controls, Agro-chemicals control and Antagonistic Fungi biological control means.But there are various defects for existing method, such as physical control mode Preventive effect is poor, and chemical agent pollution is big, is also easy to produce drug resistance, and Antagonistic Fungi preventive effect is unstable and has selectivity.
Graphene oxide (GO) is the oxide of graphene, and researches show that with potential antibacterial applications prospect.Hu etc. exists The antibacterial action of GO is reported within 2010 for the first time, this experimental result causes the extensive of numerous researchers once delivering immediately Concern.Then, the experimental results show GO to a variety of common bacterias, such as escherichia coli, staphylococcus aureus and typhoid fever Detection of Salmonella etc. all shows excellent antibacterial activity.However, GO as a kind of antibacterial agent with potential using value also by Query.The discovery such as Ruiz GO is not only unable to antibacterial, but also can be used as the skeleton of bacterial adhesion, promotes its growth, proliferation and shape At bacterial biof iotalm.In addition, not yet illustrating completely the research of the antibacterial mechanisms of GO so far.
In short, still having potential anti-according to many studies have shown thats although the anti-microbial property of GO has dispute Bacterium prospect, only its antibacterial activity has apparent selectivity.
Summary of the invention
The purpose of the present invention is overcoming the defect and deficiency of existing agriculture plant phytophthora root rot Prevention Technique, graphene oxide and wither Contracting bacillus is combined the application in terms of preventing and treating plant phytophthora germ.
Another object of the present invention is to provide a kind of novel plant phytophthora germ antibacterial agent.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
The research of the invention finds that graphene oxide has significant suppression to phytophthora root rots such as Phytophthora nicotianae Breda, phytophthora blight of pepper Production is used, and especially can play apparent synergistic function with the combination of Antagonistic Fungi atrophy bacillus, anti-to plant phytophthora root rot Effect is significant, can be applied to the biological disease-preventing preparation developed controlling plant diseases, improve crop yield, in relevant agricultural production With good popularization and application foreground.
Therefore, applying below should all be within protection scope of the present invention:
Graphene oxide and atrophy bacillus are combined the application in terms of preventing and treating plant phytophthora germ.
Graphene oxide and atrophy bacillus are combined the application in terms of preparing plant phytophthora germ antibacterial agent.
Wherein preferably, the plant phytophthora germ is peronophythora litchi (Peronophthora litchii Chen), Phytophthora nicotianae Breda (Phytophthora nicotianae), soyabean phytophthora (phytophthora sojae), cucumber Phytophthora (Phytophthora melonis) or phytophthora blight of pepper (Phytophthora capsici Leonian).
In addition, the antibacterial agent of the plant phytophthora germ containing graphene oxide and atrophy bacillus, and contain oxidation The medicament of graphene and the prevention and treatment crop phytophthora root rot of atrophy bacillus, also all should be within that scope of the present invention.
The bacteriostasis of studies have shown that graphene oxide has dose dependent.
Preferably, in the antibacterial agent, the concentration of graphene oxide is not less than 3g/L.
It is highly preferred that the concentration of graphene oxide is 3~20g/L in the antibacterial agent.
It is highly preferred that the concentration of graphene oxide is 5~15g/L in the antibacterial agent.
Preferably, the mass ratio of atrophy bacillus and graphene oxide is 100:1~15.
The present invention is explored by numerous studies, and discovery graphene oxide has selectivity to the inhibiting effect of germ, to certain The growth of a little specific biological cells is inhibited, and there is promotion to improve the growth ability of other biological cells Effect.It is tested based on a large amount of screening study, the present invention proposes that graphene oxide has excellent inhibition to a variety of phytophthora germs Activity, and can be combined with atrophy bacillus and show excellent synergistic function, it is obviously improved to plant phytophthora root rot Preventive effect.Mechanism therein still needs to further study, thus it is speculated that and it may be the growth that graphene oxide can promote Antagonistic Fungi, therefore, While playing itself inhibition phytophthora germ effect, additionally it is possible to which significant collaboration is played in the effect for promoting cause of disease Antagonistic Fungi Synergistic effect.
The invention has the advantages that:
Studies have shown that graphene oxide of the present invention to a variety of phytophthora germs have significant inhibiting effect, especially with it is short of money The combination of antibacterial atrophy bacillus can play apparent synergistic function, significant to plant phytophthora root rot preventive effect, can be applied to grind There is popularization well to answer in relevant agricultural production for controlling plant diseases processed, the biological disease-preventing preparation for improving crop yield Use prospect.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Unless stated otherwise, agents useful for same and material of the present invention are commercially available.
The anti-phytophthora activity of 1 graphene oxide of embodiment
1, experimental material
(1) for trying pathogen:Soyabean phytophthora (phytophthora sojae), Phytophthora nicotianae Breda (Phytophthora Nicotianae), peronophythora litchi (Peronophthora litchii Chen), cucumber phytophthora (Phytophthora Melonis), phytophthora blight of pepper (Phytophthora capsici Leonian).
(2) medicament:Utilize conventional hummers method preparation gained graphene oxide (GO).
2, living body pathogen mycelial growth rate method
(1) it adds graphene oxide into sterilized and cooling Pathogen culture base, mixes, be diluted to containing for series of concentrations Medicine culture medium (0.1,1,3,5,10,15,20,25g/L), every culture dish are inoculated with a bacterium dish (diameter d=0.6cm), each concentration If 3 repetitions;The culture medium of graphene oxide is not added as control (CK), using metalaxyl as positive control medicament, measurement is aoxidized Graphene is to the inhibitory activity for trying pathogen.It is handed over when 25 DEG C of cultures cover with 2/3 or more culture dish to control bacterium colony with cross Fork method measures the colony diameter (cm) of each concentration processing, its average value is taken to represent, and calculates medicament to mycelial growth inhibition ratio.
(2) the results are shown in Table 1.
Inhibiting effect of 1 graphene oxide of table to five kinds of various pathogenic bacterias
As seen from the results in Table 1, graphene oxide processing shows the mycelia growth of five kinds of various pathogenic bacterias excellent Inhibitory activity, and there is dose dependent, preferred concentration is 3~20g/L, most preferably 5~15g/L.
3, dilution plate counting method carries out the measurement of micro organism quantity
(1) it adds graphene oxide into sterilized and cooling Pathogen culture base and mixes, be diluted to containing for series of concentrations Medicine culture medium (0.1,1,3,5,10,15,20,25g/L), every culture bottle inoculation pathogen are cultivated, and each concentration sets 3 weights It is multiple;Graphene oxide (CK) is not added for control, using metalaxyl as positive control medicament.Processing is carried out in aseptic operating platform, examination Container used prevents living contaminants in operating process through high-temperature sterilization in testing.Constant temperature incubation starts counting after 3 days, continuous to count Number three days, taking bacterium number at most was once final result, as a result took 3 duplicate average values.
(2) the results are shown in Table 2.
Inhibiting effect of 2 graphene oxide of table to five kinds of various pathogenic bacterias
As seen from the results in Table 2, compared with the control group, graphene oxide processing shows five kinds of various pathogenic bacterias excellent Growth inhibitory activity, and have dose dependent.
The potted plant experiment of the anti-phytophthora of 2 graphene oxide of embodiment
1, experimental material is with embodiment 1, while graphene oxide and water being mixed, be made into a series of concentration (1,3,5,7, 9,11,13,15,17,19,21g/L) antimicrobial fluid.Then soybean, tobacco, cucumber, Hot Pepper Seedling are utilized respectively and carries out potting reality It tests.
2, experimental method
Graphene oxide antimicrobial fluid is uniformly poured in the plantling root that cotyledon is sufficiently spread out, is inoculated with each cause of disease afterwards for 24 hours Bacterium is poured once, continuous pouring 3 times, is control with clear water and blank cultures, each processing is repeated 3 times, after processing for every 7 days It is measured by sampling within 21 days, calculates preventive effect.
3, the results are shown in Table 3 for diseases prevention.
The measurement result of 3 graphene oxide of table prevention and treatment plant phytophthora root rot
The results show that graphene oxide shows good preventive effect to five kinds of different phytophthora root rots, to peronophythora litchi, Phytophthora nicotianae disease, the preventive effect of phytophthora sojae kaufmann&gerdemann are best, and have dose dependent.
Anti- phytophthora activity associated with 3 graphene oxide of embodiment and atrophy bacillus
1, experimental material
(1) pathogen:With embodiment 1.
(2) antibacterial components:Graphene oxide (GO) and Antagonistic Fungi:Atrophy bacillus (Bacillus Atrophaeus) (commercially available, viable count about 2.00 × 1010/ g), trichoderma asperellum (Trichoderma asperellum) (it is commercially available, Viable count about 1.00 × 109/g)。
2, experimental method
Respectively by Antagonistic Fungi and graphene oxide according to 100:8 mass ratio mixes, and is diluted with water to antimicrobial fluid, wherein oxygen The final concentration of 10g/L of graphite alkene.
It in addition with 10g/L graphene oxide antimicrobial fluid and metalaxyl is respectively control.
Referring next to the method for embodiment 1, living body pathogen mycelial growth rate method test bacteriostasis rate and dilute is respectively adopted Release the measurement that colony counting method carries out micro organism quantity.
3, result is as shown in table 4 and 5
Inhibiting effect of 4 each group of table to five kinds of various pathogenic bacterias
Inhibiting effect of 5 each group of table to five kinds of various pathogenic bacterias
By above-mentioned experimental result it is found that graphene oxide and Antagonistic Fungi atrophy bacillus are combined, apparent association can produce Same synergistic effect is significantly improved to the inhibitory activity of five kinds of pathogens.
Anti- phytophthora potted plant experiment associated with 4 graphene oxide of embodiment and atrophy bacillus
1, experimental material is with embodiment 3, and experimental method is with embodiment 2, by antibacterial made of Antagonistic Fungi and graphene oxide Liquid is utilized respectively soybean, tobacco, cucumber, Hot Pepper Seedling and carries out potted plant experiment.
2, the results are shown in Table 6 for diseases prevention.
The measurement result of 6 each group of table prevention and treatment plant phytophthora root rot
The results show that graphene oxide and Antagonistic Fungi atrophy bacillus are combined, apparent synergistic function can produce, It is significantly improved to the preventive effect of five kinds of plant phytophthora root rots.
The ratio optimization of embodiment 5 graphene oxide and atrophy bacillus
Using graphene oxide and the amount ratio of atrophy bacillus as variable factors, screening test is optimized.Experiment Material and method are with embodiment 3, by taking phytophthora blight of pepper as an example, test bacteriostasis rate using living body pathogen mycelial growth rate method. The results are shown in Table 7, when the mass ratio of atrophy bacillus and graphene oxide is 100:When 1~15, the preventive effect of composite drug It is best.
Inhibiting effect of 7 each group of table to five kinds of various pathogenic bacterias

Claims (7)

1. graphene oxide and atrophy bacillus are combined the application in terms of preventing and treating plant phytophthora germ.
2. graphene oxide and atrophy bacillus are combined the application in terms of preparing plant phytophthora germ antibacterial agent.
3. application according to claim 1 or 2, which is characterized in that the plant phytophthora germ is peronophythora litchi, cigarette Careless phytophthora, soyabean phytophthora, cucumber phytophthora or phytophthora blight of pepper.
4. a kind of antibacterial agent of resisting phytophthora disease bacterium, which is characterized in that contain graphene oxide and atrophy bacillus.
5. a kind of medicament for preventing and treating plant phytophthora root rot, which is characterized in that contain graphene oxide and atrophy bacillus.
6. medicament described in antibacterial agent or claim 5 according to claim 4, which is characterized in that atrophy bacillus and oxygen The mass ratio of graphite alkene is 100:1~15.
7. medicament described in antibacterial agent or claim 5 according to claim 4, which is characterized in that the phytophthora root rot is lichee White phytophthora, Phytophthora nicotianae, soybean phytophthora, cucumber phytophthora or Phytophthora capsici.
CN201810511644.5A 2018-05-24 2018-05-24 A kind of plant phytophthora root rot antibacterial agent containing graphene oxide Withdrawn CN108925577A (en)

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Application Number Priority Date Filing Date Title
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Application publication date: 20181204