CN108925577A - A kind of plant phytophthora root rot antibacterial agent containing graphene oxide - Google Patents
A kind of plant phytophthora root rot antibacterial agent containing graphene oxide Download PDFInfo
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- CN108925577A CN108925577A CN201810511644.5A CN201810511644A CN108925577A CN 108925577 A CN108925577 A CN 108925577A CN 201810511644 A CN201810511644 A CN 201810511644A CN 108925577 A CN108925577 A CN 108925577A
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- phytophthora
- graphene oxide
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- root rot
- antibacterial agent
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 55
- 229910021389 graphene Inorganic materials 0.000 title claims abstract description 55
- 241000233614 Phytophthora Species 0.000 title claims abstract description 52
- 239000003242 anti bacterial agent Substances 0.000 title claims abstract description 15
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 25
- 206010003694 Atrophy Diseases 0.000 claims abstract description 24
- 230000037444 atrophy Effects 0.000 claims abstract description 24
- 201000010099 disease Diseases 0.000 claims abstract description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 15
- 241000196324 Embryophyta Species 0.000 claims description 26
- 239000003814 drug Substances 0.000 claims description 11
- 241000233616 Phytophthora capsici Species 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 241000233645 Phytophthora nicotianae Species 0.000 claims description 7
- 240000008067 Cucumis sativus Species 0.000 claims description 6
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 244000183278 Nephelium litchi Species 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 229910002804 graphite Inorganic materials 0.000 claims description 2
- 239000010439 graphite Substances 0.000 claims description 2
- -1 graphite alkene Chemical class 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 235000019504 cigarettes Nutrition 0.000 claims 1
- 241000233866 Fungi Species 0.000 abstract description 13
- 230000003042 antagnostic effect Effects 0.000 abstract description 13
- 230000002401 inhibitory effect Effects 0.000 abstract description 12
- 244000052616 bacterial pathogen Species 0.000 abstract description 10
- 230000003449 preventive effect Effects 0.000 abstract description 10
- 230000002195 synergetic effect Effects 0.000 abstract description 7
- 238000002360 preparation method Methods 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 4
- 238000012271 agricultural production Methods 0.000 abstract description 3
- 238000013329 compounding Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 15
- 244000052769 pathogen Species 0.000 description 11
- 230000001717 pathogenic effect Effects 0.000 description 10
- 230000000844 anti-bacterial effect Effects 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 230000002265 prevention Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 230000000845 anti-microbial effect Effects 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 231100000673 dose–response relationship Toxicity 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 239000005807 Metalaxyl Substances 0.000 description 3
- 241000948155 Phytophthora sojae Species 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- ZQEIXNIJLIKNTD-UHFFFAOYSA-N methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)alaninate Chemical compound COCC(=O)N(C(C)C(=O)OC)C1=C(C)C=CC=C1C ZQEIXNIJLIKNTD-UHFFFAOYSA-N 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 241000509558 Phytophthora melonis Species 0.000 description 2
- 241001460073 Trichoderma asperellum Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 241000193410 Bacillus atrophaeus Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 208000037386 Typhoid Diseases 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000010065 bacterial adhesion Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of the plant phytophthora root rot antibacterial agent containing graphene oxide, the specifically application of graphene oxide and Antagonistic Fungi atrophy bacillus compounding in terms of preventing and treating plant phytophthora root rot.The present invention researches show that, graphene oxide has significant inhibiting effect to a variety of phytophthora germs, especially apparent synergistic function can be played with the combination of Antagonistic Fungi atrophy bacillus, it is significant to plant phytophthora root rot preventive effect, it can be applied to the biological disease-preventing preparation developed controlling plant diseases, improve crop yield, there is good popularization and application foreground in relevant agricultural production.
Description
Technical field
The invention belongs to agricultural biological technical fields.More particularly, to a kind of plant phytophthora containing graphene oxide
Sick antibacterial agent, specifically graphene oxide and Antagonistic Fungi atrophy bacillus compound the application in terms of preventing and treating plant phytophthora root rot.
Background technique
Phytophthora (Phytophthora) is mostly parasitic germ, endangers the crushing pathogen of economic plants, and induction is permitted
More important plant disease, caused disease are commonly referred to as epidemic disease.Currently, the prevention and treatment of plant phytophthora root rot mostly uses physics anti-
It controls, Agro-chemicals control and Antagonistic Fungi biological control means.But there are various defects for existing method, such as physical control mode
Preventive effect is poor, and chemical agent pollution is big, is also easy to produce drug resistance, and Antagonistic Fungi preventive effect is unstable and has selectivity.
Graphene oxide (GO) is the oxide of graphene, and researches show that with potential antibacterial applications prospect.Hu etc. exists
The antibacterial action of GO is reported within 2010 for the first time, this experimental result causes the extensive of numerous researchers once delivering immediately
Concern.Then, the experimental results show GO to a variety of common bacterias, such as escherichia coli, staphylococcus aureus and typhoid fever
Detection of Salmonella etc. all shows excellent antibacterial activity.However, GO as a kind of antibacterial agent with potential using value also by
Query.The discovery such as Ruiz GO is not only unable to antibacterial, but also can be used as the skeleton of bacterial adhesion, promotes its growth, proliferation and shape
At bacterial biof iotalm.In addition, not yet illustrating completely the research of the antibacterial mechanisms of GO so far.
In short, still having potential anti-according to many studies have shown thats although the anti-microbial property of GO has dispute
Bacterium prospect, only its antibacterial activity has apparent selectivity.
Summary of the invention
The purpose of the present invention is overcoming the defect and deficiency of existing agriculture plant phytophthora root rot Prevention Technique, graphene oxide and wither
Contracting bacillus is combined the application in terms of preventing and treating plant phytophthora germ.
Another object of the present invention is to provide a kind of novel plant phytophthora germ antibacterial agent.
Above-mentioned purpose of the present invention is achieved through the following technical solutions：
The research of the invention finds that graphene oxide has significant suppression to phytophthora root rots such as Phytophthora nicotianae Breda, phytophthora blight of pepper
Production is used, and especially can play apparent synergistic function with the combination of Antagonistic Fungi atrophy bacillus, anti-to plant phytophthora root rot
Effect is significant, can be applied to the biological disease-preventing preparation developed controlling plant diseases, improve crop yield, in relevant agricultural production
With good popularization and application foreground.
Therefore, applying below should all be within protection scope of the present invention：
Graphene oxide and atrophy bacillus are combined the application in terms of preventing and treating plant phytophthora germ.
Graphene oxide and atrophy bacillus are combined the application in terms of preparing plant phytophthora germ antibacterial agent.
Wherein preferably, the plant phytophthora germ is peronophythora litchi (Peronophthora litchii
Chen), Phytophthora nicotianae Breda (Phytophthora nicotianae), soyabean phytophthora (phytophthora sojae), cucumber
Phytophthora (Phytophthora melonis) or phytophthora blight of pepper (Phytophthora capsici Leonian).
In addition, the antibacterial agent of the plant phytophthora germ containing graphene oxide and atrophy bacillus, and contain oxidation
The medicament of graphene and the prevention and treatment crop phytophthora root rot of atrophy bacillus, also all should be within that scope of the present invention.
The bacteriostasis of studies have shown that graphene oxide has dose dependent.
Preferably, in the antibacterial agent, the concentration of graphene oxide is not less than 3g/L.
It is highly preferred that the concentration of graphene oxide is 3~20g/L in the antibacterial agent.
It is highly preferred that the concentration of graphene oxide is 5~15g/L in the antibacterial agent.
Preferably, the mass ratio of atrophy bacillus and graphene oxide is 100：1~15.
The present invention is explored by numerous studies, and discovery graphene oxide has selectivity to the inhibiting effect of germ, to certain
The growth of a little specific biological cells is inhibited, and there is promotion to improve the growth ability of other biological cells
Effect.It is tested based on a large amount of screening study, the present invention proposes that graphene oxide has excellent inhibition to a variety of phytophthora germs
Activity, and can be combined with atrophy bacillus and show excellent synergistic function, it is obviously improved to plant phytophthora root rot
Preventive effect.Mechanism therein still needs to further study, thus it is speculated that and it may be the growth that graphene oxide can promote Antagonistic Fungi, therefore,
While playing itself inhibition phytophthora germ effect, additionally it is possible to which significant collaboration is played in the effect for promoting cause of disease Antagonistic Fungi
Synergistic effect.
The invention has the advantages that：
Studies have shown that graphene oxide of the present invention to a variety of phytophthora germs have significant inhibiting effect, especially with it is short of money
The combination of antibacterial atrophy bacillus can play apparent synergistic function, significant to plant phytophthora root rot preventive effect, can be applied to grind
There is popularization well to answer in relevant agricultural production for controlling plant diseases processed, the biological disease-preventing preparation for improving crop yield
Use prospect.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Unless stated otherwise, agents useful for same and material of the present invention are commercially available.
The anti-phytophthora activity of 1 graphene oxide of embodiment
1, experimental material
(1) for trying pathogen：Soyabean phytophthora (phytophthora sojae), Phytophthora nicotianae Breda (Phytophthora
Nicotianae), peronophythora litchi (Peronophthora litchii Chen), cucumber phytophthora (Phytophthora
Melonis), phytophthora blight of pepper (Phytophthora capsici Leonian).
(2) medicament：Utilize conventional hummers method preparation gained graphene oxide (GO).
2, living body pathogen mycelial growth rate method
(1) it adds graphene oxide into sterilized and cooling Pathogen culture base, mixes, be diluted to containing for series of concentrations
Medicine culture medium (0.1,1,3,5,10,15,20,25g/L), every culture dish are inoculated with a bacterium dish (diameter d=0.6cm), each concentration
If 3 repetitions；The culture medium of graphene oxide is not added as control (CK), using metalaxyl as positive control medicament, measurement is aoxidized
Graphene is to the inhibitory activity for trying pathogen.It is handed over when 25 DEG C of cultures cover with 2/3 or more culture dish to control bacterium colony with cross
Fork method measures the colony diameter (cm) of each concentration processing, its average value is taken to represent, and calculates medicament to mycelial growth inhibition ratio.
(2) the results are shown in Table 1.
Inhibiting effect of 1 graphene oxide of table to five kinds of various pathogenic bacterias
As seen from the results in Table 1, graphene oxide processing shows the mycelia growth of five kinds of various pathogenic bacterias excellent
Inhibitory activity, and there is dose dependent, preferred concentration is 3~20g/L, most preferably 5~15g/L.
3, dilution plate counting method carries out the measurement of micro organism quantity
(1) it adds graphene oxide into sterilized and cooling Pathogen culture base and mixes, be diluted to containing for series of concentrations
Medicine culture medium (0.1,1,3,5,10,15,20,25g/L), every culture bottle inoculation pathogen are cultivated, and each concentration sets 3 weights
It is multiple；Graphene oxide (CK) is not added for control, using metalaxyl as positive control medicament.Processing is carried out in aseptic operating platform, examination
Container used prevents living contaminants in operating process through high-temperature sterilization in testing.Constant temperature incubation starts counting after 3 days, continuous to count
Number three days, taking bacterium number at most was once final result, as a result took 3 duplicate average values.
(2) the results are shown in Table 2.
Inhibiting effect of 2 graphene oxide of table to five kinds of various pathogenic bacterias
As seen from the results in Table 2, compared with the control group, graphene oxide processing shows five kinds of various pathogenic bacterias excellent
Growth inhibitory activity, and have dose dependent.
The potted plant experiment of the anti-phytophthora of 2 graphene oxide of embodiment
1, experimental material is with embodiment 1, while graphene oxide and water being mixed, be made into a series of concentration (1,3,5,7,
9,11,13,15,17,19,21g/L) antimicrobial fluid.Then soybean, tobacco, cucumber, Hot Pepper Seedling are utilized respectively and carries out potting reality
It tests.
2, experimental method
Graphene oxide antimicrobial fluid is uniformly poured in the plantling root that cotyledon is sufficiently spread out, is inoculated with each cause of disease afterwards for 24 hours
Bacterium is poured once, continuous pouring 3 times, is control with clear water and blank cultures, each processing is repeated 3 times, after processing for every 7 days
It is measured by sampling within 21 days, calculates preventive effect.
3, the results are shown in Table 3 for diseases prevention.
The measurement result of 3 graphene oxide of table prevention and treatment plant phytophthora root rot
The results show that graphene oxide shows good preventive effect to five kinds of different phytophthora root rots, to peronophythora litchi,
Phytophthora nicotianae disease, the preventive effect of phytophthora sojae kaufmann&gerdemann are best, and have dose dependent.
Anti- phytophthora activity associated with 3 graphene oxide of embodiment and atrophy bacillus
1, experimental material
(1) pathogen：With embodiment 1.
(2) antibacterial components：Graphene oxide (GO) and Antagonistic Fungi：Atrophy bacillus (Bacillus
Atrophaeus) (commercially available, viable count about 2.00 × 1010/ g), trichoderma asperellum (Trichoderma asperellum) (it is commercially available,
Viable count about 1.00 × 109/g)。
2, experimental method
Respectively by Antagonistic Fungi and graphene oxide according to 100：8 mass ratio mixes, and is diluted with water to antimicrobial fluid, wherein oxygen
The final concentration of 10g/L of graphite alkene.
It in addition with 10g/L graphene oxide antimicrobial fluid and metalaxyl is respectively control.
Referring next to the method for embodiment 1, living body pathogen mycelial growth rate method test bacteriostasis rate and dilute is respectively adopted
Release the measurement that colony counting method carries out micro organism quantity.
3, result is as shown in table 4 and 5
Inhibiting effect of 4 each group of table to five kinds of various pathogenic bacterias
Inhibiting effect of 5 each group of table to five kinds of various pathogenic bacterias
By above-mentioned experimental result it is found that graphene oxide and Antagonistic Fungi atrophy bacillus are combined, apparent association can produce
Same synergistic effect is significantly improved to the inhibitory activity of five kinds of pathogens.
Anti- phytophthora potted plant experiment associated with 4 graphene oxide of embodiment and atrophy bacillus
1, experimental material is with embodiment 3, and experimental method is with embodiment 2, by antibacterial made of Antagonistic Fungi and graphene oxide
Liquid is utilized respectively soybean, tobacco, cucumber, Hot Pepper Seedling and carries out potted plant experiment.
2, the results are shown in Table 6 for diseases prevention.
The measurement result of 6 each group of table prevention and treatment plant phytophthora root rot
The results show that graphene oxide and Antagonistic Fungi atrophy bacillus are combined, apparent synergistic function can produce,
It is significantly improved to the preventive effect of five kinds of plant phytophthora root rots.
The ratio optimization of embodiment 5 graphene oxide and atrophy bacillus
Using graphene oxide and the amount ratio of atrophy bacillus as variable factors, screening test is optimized.Experiment
Material and method are with embodiment 3, by taking phytophthora blight of pepper as an example, test bacteriostasis rate using living body pathogen mycelial growth rate method.
The results are shown in Table 7, when the mass ratio of atrophy bacillus and graphene oxide is 100：When 1~15, the preventive effect of composite drug
It is best.
Inhibiting effect of 7 each group of table to five kinds of various pathogenic bacterias
Claims (7)
1. graphene oxide and atrophy bacillus are combined the application in terms of preventing and treating plant phytophthora germ.
2. graphene oxide and atrophy bacillus are combined the application in terms of preparing plant phytophthora germ antibacterial agent.
3. application according to claim 1 or 2, which is characterized in that the plant phytophthora germ is peronophythora litchi, cigarette
Careless phytophthora, soyabean phytophthora, cucumber phytophthora or phytophthora blight of pepper.
4. a kind of antibacterial agent of resisting phytophthora disease bacterium, which is characterized in that contain graphene oxide and atrophy bacillus.
5. a kind of medicament for preventing and treating plant phytophthora root rot, which is characterized in that contain graphene oxide and atrophy bacillus.
6. medicament described in antibacterial agent or claim 5 according to claim 4, which is characterized in that atrophy bacillus and oxygen
The mass ratio of graphite alkene is 100：1~15.
7. medicament described in antibacterial agent or claim 5 according to claim 4, which is characterized in that the phytophthora root rot is lichee
White phytophthora, Phytophthora nicotianae, soybean phytophthora, cucumber phytophthora or Phytophthora capsici.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810511644.5A CN108925577A (en) | 2018-05-24 | 2018-05-24 | A kind of plant phytophthora root rot antibacterial agent containing graphene oxide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810511644.5A CN108925577A (en) | 2018-05-24 | 2018-05-24 | A kind of plant phytophthora root rot antibacterial agent containing graphene oxide |
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| Publication Number | Publication Date |
|---|---|
| CN108925577A true CN108925577A (en) | 2018-12-04 |
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|---|---|---|---|
| CN201810511644.5A Withdrawn CN108925577A (en) | 2018-05-24 | 2018-05-24 | A kind of plant phytophthora root rot antibacterial agent containing graphene oxide |
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| Country | Link |
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| CN (1) | CN108925577A (en) |
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2018
- 2018-05-24 CN CN201810511644.5A patent/CN108925577A/en not_active Withdrawn
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Application publication date: 20181204 |