CN108633905A - A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol and iprodione - Google Patents
A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol and iprodione Download PDFInfo
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- CN108633905A CN108633905A CN201810938738.0A CN201810938738A CN108633905A CN 108633905 A CN108633905 A CN 108633905A CN 201810938738 A CN201810938738 A CN 201810938738A CN 108633905 A CN108633905 A CN 108633905A
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- Prior art keywords
- iprodione
- azoles bacterium
- fluorine azoles
- acyl azanol
- bacterium acyl
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
Abstract
The invention discloses a kind of bactericidal composition and its application of fluorine-containing azoles bacterium acyl azanol and iprodione, effective active composition is made of the first active constituent fluorine azoles bacterium acyl azanol and the second active constituent iprodione, and the weight ratio of the first active constituent and the second active constituent is 1：60~60：1, preferably 1：30~30：1.This composition can be configured to the wettable powder agriculturally allowed, water dispersible granules, suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent, microcapsule suspension suspending agent, dispersible oil-suspending agent, dry suspending agent dosage form.Instant component is reasonable, and bactericidal effect is good, and its activity and bactericidal effect are not the active simple superposition of each component, compared with existing single formulation, in addition to significant bactericidal effect, and have significant synergistic effect, good to crop safety.The present invention has significant control effect to crop brown spot, gray mold, powdery mildew, anthracnose, brown rot, sclerotiniose and scab.
Description
Technical field
The present invention relates to a kind of bactericidal compositions, by the first active constituent fluorine azoles bacterium acyl azanol and the different bacterium of the second active constituent
Urea and auxiliary agent composition, belong to technical field of compounded pesticide.
Background technology
Fluorine azoles bacterium acyl azanol, English common name pydiflumetofen, chemical name：3-（Difluoromethyl）- N- methoxyl groups-
1- methyl-N- [（RS）- 1- methyl -2-（2,4,6- trichlorophenyls）Ethyl] pyrazole-4-carboxamide.Fluorine azoles bacterium acyl azanol be first just
Up to the pyrazole amide series bactericidal agent of discovery, exploitation and production, list within 2017, this is one of newest listing in SDHI series bactericidal agents
A product.Fluorine azoles bacterium acyl azanol is efficient, wide spectrum is prevented suitable for cereal, corn and soybean, rape, vegetables and special crop etc.
Many diseases including gray mold, brown spot, leaf spot, sclerotiniose, powdery mildew, head blight etc..In all chemical types
Product in, fluorine azoles bacterium acyl azanol is to leaf spot and powdery mildew activity highest；To being difficult to the disease prevented, as botrytis, core
Disease is efficient caused by the pathogens such as cup fungi and stick spore bacterium；The disease caused by sickle-like bacteria on cereal is prevented also breakthroughly, such as
Head blight etc..Moreover, fluorine azoles bacterium acyl azanol can improve crop yield and income.
Iprodione, English name iprodione, chemical name：3- (3,5- dichlorophenyls)-N- isopropyl -2,4- dioxos
Imidazoline -1- carboxylic acid amides.Iprodione can inhibit protein kinase, control the Intracellular signals of many cell functions, including carbon aquation
Close the interference effect that object is combined into fungal cell constituents.Therefore, it can inhibit the sprouting and generation of fungal spore, can also press down
Mycelia growth processed, i.e., have an impact each stage of development in the pathogen history of life.It is a kind of broad-spectrum touch-out type protective fungicide
Agent, while there is certain therapeutic effect, systemic action can be also played by root absorption, effectively prevention is to benzimidazole interior suction
The resistant fungi of fungicide.
Applicant is through retrieval, and there is presently no the reports that fluorine azoles bacterium acyl azanol and iprodione mixture prevent plant fungal disease
Road is further found by experiment that, by the mechanism of action different fluorine azoles bacterium acyl azanol and iprodione, have unexpected
Effect has very the fungal diseases such as crop brown spot, gray mold, powdery mildew, anthracnose, brown rot, sclerotiniose and scab
Good control effect, synergistic effect is apparent, and dosage is reduced, and cost accounting reduces, to crop safety.
Invention content
The purpose of the present invention is to provide a kind of component is reasonable, synergistic effect is notable, and bactericidal effect is good, and drug cost is low,
To the agricultural bactericidal composition of crop safety.
Another object of the present invention is to provide the bactericidal composition preparation formulations of fluorine-containing azoles bacterium acyl azanol and iprodione.
It is still another object of the present invention to provide above-mentioned compositions in prevention crop brown spot, gray mold, powdery mildew, anthrax
Application on the fungal diseases such as disease, brown rot, sclerotiniose and scab.
In order to overcome the defect of existing single formulation, the technical scheme is that solve in this way：
A）First active constituent fluorine azoles bacterium acyl azanol；
B）Second active constituent iprodione；
The weight ratio of first active constituent and the second active constituent is 1：60~60：1, preferably 1：30~30：1.
The sum of content of first active constituent and the second active constituent is the 1%~60% of the composition total weight, preferably
It is 1%~50%.
A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol and iprodione of the present invention, according to those skilled in the art of the present technique
The preparation formulation that known method can be prepared is wettable powder, water dispersible granules, suspending agent, suspension emulsion, aqueous emulsion, micro-
Emulsion, microcapsule suspending agent, microcapsule suspension-suspendinagent agent, dispersible oil-suspending agent, dry suspending agent.
To wettable powder, workable auxiliary agent has：Dispersant such as polycarboxylate, lignosulfonates, polyoxyethylene is poly-
Oxypropylene group ether block copolymers, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol-polyethenoxy base ether phosphate, poly alkyl alcohol
It is one or more in ethylene oxide ether phosphate, alkylphenol polyoxyethylene ether sulfonate etc.；Wetting agent such as alkyl sulfate, alkane
It is one or more in base sulfonate, alkylnaphthalene sulfonate etc.；Filler such as ammonium sulfate, urea, sucrose, glucose, diatomite, height
It is one or more in ridge soil, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, clay etc..
To water dispersible granules, workable auxiliary agent has：Dispersant such as polycarboxylate（TERSPERSE2700、T36、GY-D06
Deng）, it is lignosulfonates, one or more in alkylnaphthalene sulfonate etc.；Wetting agent for example alkyl sulfate, alkylsulfonate,
It is one or more in naphthalene sulfonate etc.；Disintegrant such as ammonium sulfate, sodium sulphate, polyvinylpyrrolidone, starch and its derivative,
It is one or more in bentonite etc.；Binder for example starch, glucose, polyvinyl alcohol, polyethylene glycol, sodium carboxymethylcellulose,
It is one or more in sucrose etc.；In filler such as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, clay
It is one or more.
To suspending agent, workable auxiliary agent has：Dispersant such as polycarboxylate, lignosulfonates, alkyl naphthalene formaldehyde condensation
Object sulfonate, alkylphenol-polyethenoxy base ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene groups
Ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, pull open powder, dodecyl
It is one or more in polyoxyethylene ether phosphate etc.；Wetting agent such as alkylphenol polyoxyethylene ether formaldehyde condensate sulfate,
Phenethyl phenol polyoxyethylene groups ether phosphate, alkyl sulfate, alkylsulfonate, alkylnaphthalene sulfonate, alkylphenol-formaldehyde resin
It is one or more in polyoxyethylene ether, triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer etc.；Thickener is such as yellow
One kind in virgin rubber, polyvinyl alcohol, bentonite, aluminium-magnesium silicate, carboxymethyl cellulose, carboxyethyl cellulose, methylcellulose etc.
Or it is a variety of；It is one or more in preservative such as formaldehyde, benzoic acid, sodium benzoate, potassium sorbate, isothiazolinone；Defoaming
Agent such as silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 aliphatic alcohols, hexanol, butanol, octanol etc.
In it is one or more；In antifreezing agent such as ethylene glycol, propylene glycol, glycerine, polyethylene glycol, sorbierite, urea, inorganic salts
It is one or more；Water is deionized water.
To suspension emulsion, workable auxiliary agent has：Emulsifier such as calcium dodecyl benzene sulfonate（Agriculture breast 500#）, Nongru-700 #,
Agriculture breast 2201, this disk -60#, emulsifier T-60（Common name：Anhydrous sorbitol single-hard ester acid ester polyoxyethylene ether）、TX-10（It is logical
With name：Octylphenol polyethylene ethylene oxide（10）Ether）, it is agriculture breast 1601#, agriculture breast 600#, one or more in agriculture breast 400#；Dispersant is such as
It is one or more in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate；Solvent such as repefral, adjacent benzene two
It is one or more in formic acid dibutyl ester, ethyl acetate, methyl benzoate；Antifoaming agent is as such as silicone oil, silicone compound, C10-20
It is one or more in saturated fat acids compound, C8-10 aliphatic alcohols, hexanol, butanol, octanol；Thickener such as white carbon,
It is one or more in polyvinyl alcohol, bentonite, aluminium-magnesium silicate；Antifreeze such as ethylene glycol, propylene glycol, glycerine, urea, inorganic salts
As one or more in sodium chloride；Water is deionized water.
To aqueous emulsion, workable auxiliary agent has：Emulsifier such as Nonyl pheno（EO=10）Ether phosphate, triphen second
Base phenol polyoxyethylene phosphonate, Nongru-700 #（Alkyl phenol formaldehyde resin polyoxyethylene ether）, agriculture breast 2201#, this disk -60#
（Span60）, Tween-60 #（Polyoxyethylene sorbitan stearate）、TX-10（Octyl phenol polyoxy second
Alkene（10）Ether）, agriculture breast 1601（Triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer）, agriculture breast 600#, agriculture breast 400#
It is one or more in；Solvent such as dimethylbenzene, toluene, cyclohexanone, solvent naphtha（The trade mark：S-150、S-180、S-200）In
It is one or more；It is one or more in stabilizer such as triphenyl phosphite, epoxychloropropane, epoxidized soybean oil；It is antifreeze
It is one or more in agent such as ethylene glycol, propylene glycol, glycerine, urea, inorganic salts such as sodium chloride；Thickener such as xanthans,
It is one or more in polyvinyl alcohol, bentonite, aluminium-magnesium silicate etc.；One in preservative such as formaldehyde, benzoic acid, sodium benzoate
Kind is a variety of, and water is deionized water.
To microemulsion, workable auxiliary agent has：Emulsifier such as agriculture breast 500#（Calcium dodecyl benzene sulfonate）, Nongru-700 #,
Agriculture breast 2201, this disk-60#, polysorbate60-#, TX-10, agriculture breast 1601（Phenethyl phenol polyoxyethylene poly-oxygen propylene aether）, agriculture breast
It is one or more in 600#, agriculture breast 400# etc.；One kind or more in assistant for emulsifying agent such as methanol, isopropanol, n-butanol, ethyl alcohol
Kind；Solvent such as cyclohexanone, N-Methyl pyrrolidone, dimethylbenzene, toluene, solvent naphtha（The trade mark：S-150、S-180、S-200）In
It is one or more；It is one or more in stabilizer such as triphenyl phosphite, epoxychloropropane；Water is deionized water.
To microcapsule suspending agent, workable auxiliary agent has：Macromolecule cyst material for example polyfunctional group acyl halide polyamines, gelatin,
It is one or more in Arabic gum, deformation lactoprotein, sodium alginate；Dispersant such as fatty alcohol polyoxyethylene ether, alkyl phenol are poly-
It is one or more in the phonetic formaldehyde condensation products sulfate of ethylene oxide, alkylbenzene sulfonate；Solvent such as aliphatic ester, linseed oil, oleic acid
It is one or more in methyl esters；Emulsifier such as benzyl dimethyl phenol polyoxyethyl ether, phenethyl phenol polyoxyethanyl polypropylene-base ether, ring
It is one or more in oxidative ethane-propylene oxide block copolymer；PH conditioning agents such as sodium hydroxide and hydrochloric acid, citric acid, sorbic acid
In it is one or more；It is a kind of or more in antifoaming agent such as C8-10 aliphatic alcohols, amide, organic silicon defoamer, hexanol, butanol, octanol
Kind；Water is deionized water.
To microcapsule suspension-suspendinagent agent, workable auxiliary agent has：Disintegrant such as bentonite, urea, ammonium sulfate, polyethylene pyrrole
It is one or more in pyrrolidone, glucose, sodium bicarbonate；Preservative such as formaldehyde, benzoic acid, sodium benzoate, potassium sorbate, different
It is one or more in thiazolinone etc.；Emulsifier such as fatty alcohol polyoxyethylene ether, aliphatic acid polyethenoxy ether, detergent alkylate
Sulfoacid calcium, alkyl phenol polyoxyethylene ether, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, sorbitol anhydride list are hard
It is one or more in resin acid ester, sorbitan monostearate polyoxyethylene ether, aliphatic acid polyethenoxy ether phosphate etc.；
Dispersant such as polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate（SOPA）, naphthalene and alkane
Base naphthalene formaldehyde condensate sulfonates class, EO-PO block polyethers class, alkylphenol-polyethenoxy base ether phosphate, alkyl phenol polyoxy second
One kind or more in alkene ether, polyoxyethylene carboxylate, aliphatic amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ethe etc.
Kind；Wetting agent such as lauryl sodium sulfate, neopelex, Nekal BX, moistening bleeding agent F, naphthalene sulphonate formaldehyde
It is one or more in condensation product etc.；It is one or more in antifreezing agent such as urea, ethylene glycol, propylene glycol, glycerine；Thickening
One kind in agent such as xanthans, carboxymethyl cellulose, carboxyethyl cellulose, methylcellulose, aluminium-magnesium silicate, polyvinyl alcohol or
It is a variety of；Antifoaming agent such as organic silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 fatty alcohols, ester ether
It is one or more in class etc..
To dispersible oil-suspending agent, workable auxiliary agent has：Dispersant such as polycarboxylate, lignosulfonates, alkylnaphthalene
Sulfonate（Dispersing agent NNO）、TERSPERSE2020（Huntsman Corporation of U.S. HUNTSMAN produces, alkyl naphthalene sulfonic acid salt）In
It is one or more；Emulsifier such as BY（Castor oil polyoxyethylene ether）Serial emulsifier（BY-110、BY-125、BY-140）, agriculture breast
700#（Common name：Alkyl phenol formaldehyde resin polyoxyethylene ether）, agriculture breast 2201, this disk -60#（Common name：Sorbitol anhydride list is stearic
Acid esters）, Tween-60 #（Common name：Sorbitan monostearate polyoxyethylene ether）, agriculture breast 1601#（Common name：Phenethyl
Phenol polyoxyethylene poly-oxygen propylene aether）、TERSPERSE4894（Huntsman Corporation of the U.S. produces）In it is one or more；Wetting
Agent such as alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkyl phenol polyoxyethylene ether phosphate, phenethyl phenol polyoxy second
Alkenyl ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE2500（Huntsman Corporation of the U.S. produces）
In it is one or more；It is one or more in thickener such as white carbon, polyvinyl alcohol, bentonite, aluminium-magnesium silicate；Antifreezing agent such as second two
It is one or more in alcohol, propylene glycol, glycerine, urea, inorganic salts such as sodium chloride；Decentralized medium such as soybean oil, rapeseed oil, wheat
It is one or more in oil, methyl oleate, diesel oil, machine oil, mineral oil.
To dry suspending agent, workable auxiliary agent has：Dispersant such as lauryl sodium sulfate, polycarboxylate, acrylic acid homopolymerization
Oxygen sodium salt, dioctyl sodium sulphosuccinate, Teepol, neopelex, fatty acid polyethylene glycol ester, alkane acyl
Amido taurate, hydroxypropyl cellulose sodium, fatty alcohol polyoxyethylene ether, sodium naphthalene sulfonate, Negel, aliphatic acid sulphur alkane
It is one or more in base ester, lignosulfonates etc.；Wetting agent such as lauryl sodium sulfate, Negel, dodecane
One kind in base benzene sulfonic acid sodium salt, sodium lignin sulfonate, Negel formaldehyde condensation products, alkylphenol-polyethenoxy base ether etc. or
It is a variety of；One kind in disintegrant such as phosphate, sulfate, carbonate, urea, sodium chloride, bentonite, polyvinylpyrrolidone
Or it is a variety of；One in binder such as Arabic gum, carboxymethyl cellulose, polyvinyl alcohol, polyethylene glycol, polyvinylpyrrolidone
Kind is a variety of；Antifoaming agent for example silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 aliphatic alcohols, oneself
It is one or more in alcohol, butanol, octanol etc.；Carrier such as diatomite, precipitated calcium carbonate, tripoli, kaolin, white carbon, bumps
It is one or more in stick soil etc..
Instant component is reasonable, and treatment plus protective effect, bactericidal effect is good, and drug cost is low, and its activity and sterilization effect
The active simple superposition of fruit not instead of each component, there is significant synergistic effect, good to crop safety, meets pesticidal preparations
Security requirement.The present invention is true to crop brown spot, gray mold, powdery mildew, anthracnose, brown rot, sclerotiniose and scab etc.
Fungus diseases have excellent control effect.
Specific implementation mode
1, the mixture synergy of brown spot is measured.
In order to prevent the brown spot in agricultural production, we have carried out mutual compounding with fluorine azoles bacterium acyl azanol and iprodione
Synergy is studied, and the specific method is as follows.
Subjects are marssonina coronaria（Marssonina coronaria）.The investigational agent that active compound is configured to need
Agent, test method is with reference to the concave slide method progress for inhibiting disease fungus spore germination test.
It is spare after spore to be generated by apple marssonina leaf spot diseased tissues moisturizing culture.By apple marssonina leaf spot spore deionization
Water is eluted from diseased tissues, is filtered, centrifugation（1000r/min）5min removes supernatant, deionized water is added, then centrifuge.Finally
Spore is resuspended floating to 1 × 10 with deionized water5A~1 × 107A spore, and 0.5% glucose sugar juice is added.
5 various concentration gradients are arranged in single dose and each Mixed Pharmacy first（On the basis of pilot study, bacteriostasis rate exists
It is set by geometric progression in the range of 5%~90%）.With pipette or pipettor from low concentration to high concentration, liquid is drawn successively
0.5mL is separately added into small test tube, is then drawn the spore suspension 0.5mL prepared, is made liquid and spore suspension equivalent
It is uniformly mixed.Above-mentioned mixed liquor is drawn with micro sample adding appliance to drip on concave slide, then frame is put in the culture dish with shoaling layer
In, capping moisturizing culture is in the incubator of preference temperature.If blank control is done in the processing without medicament, often processing is repeated 4 times.
When blank control spore germination rate reaches 90% or more, each processing spore germination situation is checked.Often handle each repetition with
Machine observes 3 or more the visuals field, and investigation spore sum is no less than 200, records spore germination number and spore sum respectively.Spore bud
The short radius that length of tube is more than spore is considered as sprouting.According to survey data, the spore germination relative inhibition of each processing is calculated, it is single
Position is percentage（%）.
Spore germination rate（%）Spore sum × 100 of=spore germination number/investigation
Processing correction spore germination rate（%）=processing spore germination rate/blank control spore germination rate × 100
Spore germination relative inhibition（%）=（The spore germination rate of blank control spore germination rate-processing correction）/ blank control
Spore germination rate × 100
Make regression analysis according to each drug concentration logarithm and corresponding spore germination relative inhibition niqueMin, finds out each medicament
EC50 values.The co-toxicity coefficient of mixture is calculated with Sun Yunpei's Method（CTC）, activity of the reagent agent to germ is evaluated with this.It is multiple
The co-toxicity coefficient of preparaton（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜ CTC ＜, 120 tables
It is now summation action.
1 fluorine azoles bacterium acyl azanol of table measures the mixture synergy of apple marssonina leaf spot with iprodione
Synergy measurement result shows：Fluorine azoles bacterium acyl azanol presses 1 with iprodione:80~80:1 is mixed, to marssonina coronaria
Show different activity：When ratio is 1:60~60:When 1, synergistic effect is shown as；When ratio is 1:30~30:When 1, increase
Effect effect becomes apparent from.
2, the mixture synergy of gray mold is measured.
In order to prevent the gray mold in agricultural production, we have carried out mutual compounding with fluorine azoles bacterium acyl azanol and iprodione
Synergy is studied, and the specific method is as follows.
Experiment uses graw mold of tomato（Botrytis cinerea）For test object.The experiment that active compound is configured to need
5 various concentration gradients are arranged in single dose and each Mixed Pharmacy by medicament（On the basis of pilot study result, bacteriostasis rate is 5%
It is set by geometric progression in the range of~90%）.If clear water compares, it is repeated 4 times.Experiment uses mycelial growth rate method, will cultivate
Good pathogen cuts bacteria cake aseptically with a diameter of 5mm card punch from colony edge, is connect bacteria cake with transfer needle
In on the culture medium flat plate of different agents concentration, setting 22 DEG C, cultivate in saturated humidity incubator；When control group bacterium colony covers with, survey
Colony diameter is measured, the percentage that each chemicals treatment inhibits mycelia growth is calculated, passes through the probit value of inhibiting rate and series concentration
Linear regression analysis between logarithm finds out the EC of each medicament50Value calculates the co-toxicity coefficient of mixture with Sun Yunpei's Method
（CTC）, activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient of complex preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜
CTC ＜ 120 show as summation action.
2 fluorine azoles bacterium acyl azanol of table measures the mixture synergy of graw mold of tomato with iprodione
Synergy measurement result shows：Fluorine azoles bacterium acyl azanol presses 1 with iprodione:80~80:1 is mixed, to botrytis cinerea
Show different activity：When ratio is 1:60~60:When 1, synergistic effect is shown as；When ratio is 1:30~30:When 1, increase
Effect effect becomes apparent from.
3, the mixture synergy of powdery mildew is measured.
In order to prevent the powdery mildew in agricultural production, we have carried out mutual compounding with fluorine azoles bacterium acyl azanol and iprodione
Synergy is studied, and the specific method is as follows.
Subjects are cucumber powdery mildew's pathogen（Sphaerotheca fuliginea）.The experiment that active compound is configured to need
Medicament, test method reference《The 6th part of farm-chemical indoor determination test rule fungicide：The synergy of mixture measures》With
《The 11st part of farm-chemical indoor determination test rule fungicide：It prevents cucurbits powdery mildew and tests pot-culture method》.
5 various concentration gradients are arranged in single dose and each Mixed Pharmacy first（On the basis of pilot study, bacteriostasis rate exists
It is set by geometric progression in the range of 5%~90%）.If blank control is repeated 4 times.Using pot-culture method, liquid is uniformly sprayed on
Blade face, it is spare after liquid natural air drying.With spore suspension spray inoculation, then natural air drying moves to thermostatic chamber, in temperature
Under conditions of 20~24 DEG C, 7d~10d is cultivated.When blank control disease leaf rate reaches 80% or more, classification investigation is got a haircut everywhere
State of an illness condition often handles at least 30 leaves of investigation.Grade scale is：
0 grade：It is disease-free；
1 grade：Lesion area accounts for 5% or less entire leaf area；
3 grades：Lesion area accounts for the 5%~15% of entire leaf area；
5 grades：Lesion area accounts for the 15%~25% of entire leaf area；
7 grades：Lesion area accounts for the 25%~50% of entire leaf area；
9 grades：Lesion area accounts for the 50%~75% of entire leaf area；
11 grades：Lesion area accounts for 75% or more of entire leaf area；
According to survey data, the disease index and control effect of each processing are calculated.Pass through pair of preventive effect niqueMin and series concentration
Linear regression analysis between numerical value finds out the EC50 values of each medicament.
The co-toxicity coefficient of mixture is calculated with Sun Yunpei's Method（CTC）, activity of the reagent agent to germ is evaluated with this.Compounding
The co-toxicity coefficient of preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜ CTC ＜ 120 are showed
For summation action.
3 fluorine azoles bacterium acyl azanol of table measures the mixture synergy of powdery mildew of cucumber with iprodione
Synergy measurement result shows：Fluorine azoles bacterium acyl azanol presses 1 with iprodione:80~80:1 is mixed, to cucumber powdery mildew's pathogen
Show different activity：When ratio is 1:60~60:When 1, synergistic effect is shown as；When ratio is 1:30~30:When 1, increase
Effect effect becomes apparent from.
4, the mixture synergy of anthracnose is measured.
In order to prevent the anthracnose in agricultural production, we have carried out mutual compounding with fluorine azoles bacterium acyl azanol and iprodione
Synergy is studied, and the specific method is as follows.
Experiment uses citrus anthracnose（Colletotrichum gloeosporioides）For test object.Active compound is matched
The test medicine of needs is made, 5 various concentration gradients are arranged in single dose and each Mixed Pharmacy（On the basis of pilot study result
On, bacteriostasis rate is set in the range of 5%~90% by geometric progression）.If clear water compares, it is repeated 4 times.Experiment is grown using mycelia
Cultured pathogen aseptically with a diameter of 5mm card punch, bacteria cake is cut from colony edge by performance rate method, with connecing
Bacteria cake is connected on the culture medium flat plate of different agents concentration by kind of needle, is set 26 DEG C, is cultivated in saturated humidity incubator；Wait for control group bacterium
Fall when covering with, measure colony diameter, calculate the percentage that each chemicals treatment inhibits mycelia growth, by the probit value of inhibiting rate and
Linear regression analysis between the logarithm of series concentration finds out the EC of each medicament50Value calculates being total to for mixture with Sun Yunpei's Method
Malicious coefficient（CTC）, activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient of complex preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜
CTC ＜ 120 show as summation action.
4 fluorine azoles bacterium acyl azanol of table measures the mixture synergy of citrus anthracnose with iprodione
Synergy measurement result shows：Fluorine azoles bacterium acyl azanol presses 1 with iprodione:80~80:1 is mixed, to citrus anthracnose bacterium
Show different activity：When ratio is 1:60~60:When 1, synergistic effect is shown as；When ratio is 1:30~30:When 1, increase
Effect effect becomes apparent from.
5, the mixture synergy of brown rot is measured.
In order to prevent the brown rot in agricultural production, we have carried out mutual compounding with fluorine azoles bacterium acyl azanol and iprodione
Synergy is studied, and the specific method is as follows.
Experiment uses peach brown rot（Monilinia laxa）For test object.The investigational agent that active compound is configured to need
5 various concentration gradients are arranged in single dose and each Mixed Pharmacy by agent（On the basis of pilot study result, bacteriostasis rate 5%~
It is set by geometric progression in the range of 90%）.If clear water compares, it is repeated 4 times.Experiment uses mycelial growth rate method, will cultivate
Pathogen cut bacteria cake from colony edge aseptically with a diameter of 5mm card punch, bacteria cake is connected to transfer needle
On the culture medium flat plate of different agents concentration, 25 DEG C are set, cultivate in saturated humidity incubator；When control group bacterium colony covers with, measure
Colony diameter calculates the percentage that each chemicals treatment inhibits mycelia growth, passes through the probit value of inhibiting rate and pair of series concentration
Linear regression analysis between numerical value finds out the EC of each medicament50Value calculates the co-toxicity coefficient of mixture with Sun Yunpei's Method（CTC）,
Activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient of complex preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜
CTC ＜ 120 show as summation action.
5 fluorine azoles bacterium acyl azanol of table measures the mixture synergy of peach brown rot with iprodione
Synergy measurement result shows：Fluorine azoles bacterium acyl azanol presses 1 with iprodione:80~80:1 is mixed, to Monilinia fructicola table
Reveal different activity：When ratio is 1:60~60:When 1, synergistic effect is shown as；When ratio is 1:30~30:When 1, synergy
Effect becomes apparent from.
6, the mixture synergy of sclerotiniose is measured.
In order to prevent the sclerotiniose in agricultural production, we have carried out mutual compounding with fluorine azoles bacterium acyl azanol and iprodione
Synergy is studied, and the specific method is as follows.
Experiment uses Sclerotinia sclerotiorum（Sclerotinia sclerotiorum）For test object.Active compound is configured to
5 various concentration gradients are arranged in single dose and each Mixed Pharmacy by the test medicine needed（On the basis of pilot study result,
Bacteriostasis rate is set in the range of 5%~90% by geometric progression）.If clear water compares, it is repeated 4 times.Experiment is using mycelia growth speed
Cultured pathogen aseptically with a diameter of 5mm card punch, is cut bacteria cake, with inoculation by rate method from colony edge
Bacteria cake is connected on the culture medium flat plate of different agents concentration by needle, is set 20 DEG C, is cultivated in saturated humidity incubator；Wait for control group bacterium colony
When covering with, colony diameter is measured, calculates the percentage that each chemicals treatment inhibits mycelia growth, by the probit value of inhibiting rate and is
Linear regression analysis between the logarithm of row concentration finds out the EC of each medicament50Value calculates the total poison of mixture with Sun Yunpei's Method
Coefficient（CTC）, activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient of complex preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜
CTC ＜ 120 show as summation action.
6 fluorine azoles bacterium acyl azanol of table measures the mixture synergy of sclerotinia sclerotiorum with iprodione
Synergy measurement result shows：Fluorine azoles bacterium acyl azanol presses 1 with iprodione:80~80:1 is mixed, to Sclerotinia sclerotiorum
Show different activity：When ratio is 1:60~60:When 1, synergistic effect is shown as；When ratio is 1:30~30:When 1, increase
Effect effect becomes apparent from.
7, the mixture synergy of scab is measured.
In order to prevent the scab in agricultural production, we have carried out mutual compounding with fluorine azoles bacterium acyl azanol and iprodione
Synergy is studied, and the specific method is as follows.
Experiment uses pear cucumerinum（Venturia pirina）For test object.The experiment that active compound is configured to need
5 various concentration gradients are arranged in single dose and each Mixed Pharmacy by medicament（On the basis of pilot study result, bacteriostasis rate is 5%
It is set by geometric progression in the range of~90%）.If clear water compares, it is repeated 4 times.Experiment uses mycelial growth rate method, will cultivate
Good pathogen cuts bacteria cake aseptically with a diameter of 5mm card punch from colony edge, is connect bacteria cake with transfer needle
In on the culture medium flat plate of different agents concentration, setting 21 DEG C, cultivate in saturated humidity incubator；When control group bacterium colony covers with, survey
Colony diameter is measured, the percentage that each chemicals treatment inhibits mycelia growth is calculated, passes through the probit value of inhibiting rate and series concentration
Linear regression analysis between logarithm finds out the EC of each medicament50Value calculates the co-toxicity coefficient of mixture with Sun Yunpei's Method
（CTC）, activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient of complex preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜
CTC ＜ 120 show as summation action.
7 fluorine azoles bacterium acyl azanol of table measures the mixture synergy of pear scab with iprodione
Synergy measurement result shows：Fluorine azoles bacterium acyl azanol presses 1 with iprodione:80~80:1 is mixed, to pear cucumerinum table
Reveal different activity：When ratio is 1:60~60:When 1, synergistic effect is shown as；When ratio is 1:30~30:When 1, synergy
Effect becomes apparent from.
In order to better illustrate the present invention, the content of present invention is described further with reference to embodiment, in formula
Percentage is weight percentage.
Example of formulations 1
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 5% alkylphenol-polyethenoxy base ether sulfonic acid sodium, 5% sodium lignin sulfonate, 4%
Sodium alkyl sulfate, fine particle calcium carbonate are filled up to 100% parts by weight.Above-mentioned raw materials are through mixing, ultra micro air-flow crushing, hybrid technique step system
It is standby to obtain 22% fluorine azoles bacterium acyl azanol iprodione wettable powder.
Example of formulations 2
Weigh 5% fluorine azoles bacterium acyl azanol, 25% iprodione, 6% sodium polycarboxylate, 4% alkyl naphthalene formaldehyde condensation product sodium sulfonate, 4% dodecane
Base sodium sulphate, sucrose are filled up to 100% parts by weight.Above-mentioned raw materials are prepared through mixing, ultra micro air-flow crushing, hybrid technique step
Obtain 30% fluorine azoles bacterium acyl azanol iprodione wettable powder.
Example of formulations 3
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 5% polycarboxylate T36,4% Negel, 3% Nekal BX（Two fourths
Base sodium naphthalene sulfonate）, 2% lauryl sodium sulfate, 3% bentonite, clay adds to 100% parts by weight.The frequent regulation water intaking of above-mentioned raw materials
The method of dispersible granule i.e. mixing, ultra micro air-flow crushing, mixing, granulation step produces 22% fluorine azoles bacterium acyl azanol iprodione water
Dispersible granule.
Example of formulations 4
Weigh 5% fluorine azoles bacterium acyl azanol, 20% iprodione, 6% Negel, 3% polycarboxylate TERSPERSE2700,2% drawing
Open powder BX（Nekal）, 2% sodium naphthalene sulfonate, 3% polyethylene glycol, talcum powder adds to 100% parts by weight.Above-mentioned raw materials pass through
The method that routine produces water dispersible granules mixes, ultra micro air-flow crushing, mixing, granulation step produce 25% fluorine azoles bacterium acyl hydroxyl
Amine iprodione water dispersible granules.
Example of formulations 5
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 5% phenethyl phenol polyoxyethylene groups ether phosphate, 4% sodium lignin sulfonate,
5% alkyl phenol formaldehyde resin polyoxyethylene ether sodium, 1% polyvinyl alcohol, 3% isothiazolinone, 1% potassium sorbate, 2% silicone oil, deionization
Water adds to 100% parts by weight.Above-mentioned raw materials disperse 30min, are sanded to grain size D with sand mill through mixing, high speed shear90Less than 10 μ
22% fluorine azoles bacterium acyl azanol iprodione suspending agent is made after m.
Example of formulations 6
It is poly- to weigh 5% fluorine azoles bacterium acyl azanol, 15% iprodione, 5% alkyl naphthalene formaldehyde condensation product sodium sulfonate, 7% alkylphenol-formaldehyde resin
Ethylene oxide ether, 5% Negel, 2% bentonite, 3% polyethylene glycol, 3% isothiazolinone, 1% octanol, deionized water add to
100% parts by weight.Above-mentioned raw materials disperse 30min, are sanded to grain size D with sand mill through mixing, high speed shear90It is made after less than 10 μm
Obtain 20% fluorine azoles bacterium acyl azanol iprodione suspending agent.
Example of formulations 7
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 6% agriculture breast 2201,5% dispersing agent NNO, 4% Negel, 3% castor-oil plant
Oil, 4% methyl oleate, 4% agriculture breast 600#, 2% aluminium-magnesium silicate, 4% glycerine, 1% epoxychloropropane, 0.6% benzoic acid, 0.7% organosilicon
Antifoaming agent S-29, deionized water add to 100% parts by weight.Above-mentioned raw materials are uniformly mixed, and high speed shear disperses 30min, uses sand mill
22% fluorine azoles bacterium acyl azanol iprodione suspension emulsion is made after sand milling.
Example of formulations 8
Weigh 3% fluorine azoles bacterium acyl azanol, 15% iprodione, 4% sodium lignin sulfonate, 6% methyl oleate, 5% soybean oil, 4% agriculture breast
1601#, 3% Tween-60 #, 4% alkylphenol-polyethenoxy base ether phosphate, 2 polyvinyl alcohol, 1% xanthans, 5% propylene glycol, 3% Asia
Triphenyl phosphate, 1% sodium benzoate, 0.8% hexanol, deionized water add to 100% parts by weight.Above-mentioned raw materials are uniformly mixed, and are cut at a high speed
Cutting dissipates 30min, and 18% fluorine azoles bacterium acyl azanol iprodione suspension emulsion is made after being sanded with sand mill.
Example of formulations 9
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 5% agriculture breast 600#, 4% triphenoethyl benzene phenol polyethenoxy ether phosphate, 4%
Agriculture breast 2201#, 3% this disk -60#, 1.6% epoxidized soybean oil, 2% sodium chloride, 3% toluene, 0.5% polyvinyl alcohol, 0.5% benzoic acid
Sodium, deionized water add to 100% parts by weight.For above-mentioned raw materials through mixing, it is different that 22% fluorine azoles bacterium acyl azanol is made in high speed shearing emulsification
Bacterium urea aqueous emulsion.
Example of formulations 10
Weigh 5% fluorine azoles bacterium acyl azanol, 25% iprodione, 4% agriculture breast 1601,5% agriculture breast 600#, 3% triphenoethyl benzene phenol polyethenoxy
Ether phosphate, 3% propylene glycol, 5% solvent naphtha S-200,5% sodium benzoate, 1% bentonite, 1.2% formaldehyde, deionized water add to 100%
Parts by weight.30% fluorine azoles bacterium acyl azanol iprodione aqueous emulsion is made through mixing, high speed shearing emulsification in above-mentioned raw materials.
Example of formulations 11
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 4% Nongru-700 #, 6% agriculture breast 2201,3% polysorbate60-#, 8% cyclohexanone, 3%
Dimethylbenzene, 4% ethyl alcohol, 4% triphenyl phosphite, through dissolving completely and being uniformly mixed, deionized water adds to 100% parts by weight, stirring
22% fluorine azoles bacterium acyl azanol iprodione microemulsion is made afterwards.
Example of formulations 12
Weigh 2% fluorine azoles bacterium acyl azanol, 24% iprodione, 6% Nongru-700 #, 5% agriculture breast 600#, 5% toluene, 3%N- crassitudes
Ketone, 4% ethyl alcohol, 2% epoxychloropropane, through dissolving completely and being uniformly mixed, deionized water adds to 100% parts by weight, is made after stirring
26% fluorine azoles bacterium acyl azanol iprodione microemulsion.
Example of formulations 13
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 4% sodium alginate, 6% fatty alcohol polyoxyethylene ether, 4% linseed oil, 5%
Phenethyl phenol polyoxyethanyl polypropylene-base ether, 3% sodium hydroxide and citric acid, 2% octanol, deionized water add to 100% parts by weight.
Active constituent, macromolecule cyst material, solvent are mixed into homogeneous oil phase, oil phase is added in aqueous phase solution, shearing dispersion
22% fluorine azoles bacterium acyl azanol iprodione microcapsule suspending agent is made after curing afterwards.
Example of formulations 14
Weigh 5% fluorine azoles bacterium acyl azanol, 20% iprodione, 6% gelatin, the phonetic formaldehyde condensation products sulfate of 5% alkylphenol-polyethenoxy, 5%
Hexanol, 4% benzyl dimethyl phenol polyoxyethyl ether, 3% sodium hydroxide and hydrochloric acid, 1% hexanol, deionized water add to 100% parts by weight.
Active constituent, macromolecule cyst material, solvent are mixed into homogeneous oil phase, oil phase is added in aqueous phase solution, shearing dispersion
25% fluorine azoles bacterium acyl azanol iprodione microcapsule suspending agent is made after curing afterwards.
Example of formulations 15
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 6% sodium bicarbonate, 4% benzoic acid, 8% sorbitan monostearate, 6%
Sorbitan monostearate, 5% aliphatic amine polyoxyethylene ether, 5% propylene glycol, 1% xanthans, 1.2% butanol, deionized water are supplied
Surplus.Sodium bicarbonate and benzoic acid are added in the reaction kettle with frequency control, the pH of solution is adjusted with sodium bicarbonate solution
It is worth 7.5~8.5 or so, then heats to 55~70 DEG C, stable urea resin prepolymer is obtained by the reaction.It takes a certain amount of different
Bacterium urea is dissolved in solvent, is added to the water after addition emulsifier for mixing is uniform, under high shear homogeneous, is made into O/ containing emulsifying dispersant
W type stable emulsions.Above-mentioned urea resin prepolymer is added in lotion, pH value is adjusted, polymerize under acid catalysed conditions
Reaction, makes oil phase substance be wrapped, and forms microcapsule granule.Slowly heating, solidification, temperature are controlled at 45~55 DEG C, Gu
Change 1~1.5h of time, add dispersant, microcapsule suspending agent containing iprodione is first made；By fluorine azoles bacterium acyl azanol and water and other
Auxiliary agent is sanded in sand mill after reaction kettle shearing uniformly, makes suspending agent grain size D90 at 5 μm hereinafter, can be made into fluorine-containing
The suspending agent of azoles bacterium acyl azanol.The suspending agent of fluorine-containing azoles bacterium acyl azanol and the micro-capsule suspension containing iprodione are mixed in proportion
It stirs evenly, you can 22% fluorine azoles bacterium acyl azanol iprodione microcapsule suspension-suspendinagent agent is made.
Example of formulations 16
Weigh 5% fluorine azoles bacterium acyl azanol, 10% iprodione, 4% ammonium sulfate, 6% potassium sorbate, 10% sorbitan monostearate, 7%
Calcium dodecyl benzene sulfonate, 6% alkyl phenol polyoxyethylene ether, 4% glycerine, 3% aluminium-magnesium silicate, 1% octanol, deionized water are supplied remaining
Amount.According to the processing method of example of formulations 15, you can 15% fluorine azoles bacterium acyl azanol iprodione microcapsule suspension-suspendinagent agent is made.
Example of formulations 17
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 6% sodium polycarboxylate, 5%BY-125,5%TERSPERSE4894,6% agriculture breast
2201#, 5% phenethyl phenol polyoxyethylene groups ether phosphate, 3% polyvinyl alcohol, 3% bentonite, machine oil add to 100% parts by weight.On
Raw material is stated through mixing, high speed shear disperses 30min, and 22% fluorine azoles bacterium acyl azanol iprodione is made after being sanded with sand mill to be divided
Dissipate oil-suspending agent.
Example of formulations 18
Weigh 4% fluorine azoles bacterium acyl azanol, 24% iprodione, 8% sodium lignin sulfonate, 7% agriculture breast 1601#, 4%BY-125,4% naphthalene sulfonic acids
Sodium, 3% aluminium-magnesium silicate, 3% propylene glycol, methyl oleate add to 100% parts by weight.Above-mentioned raw materials are through mixing, high speed shear dispersion
30min makes the 28% dispersible oil-suspending agent of fluorine azoles bacterium acyl azanol iprodione after being sanded with sand mill.
Example of formulations 19
Weigh 2% fluorine azoles bacterium acyl azanol, 20% iprodione, 6% fatty alcohol polyoxyethylene ether, 3% Negel formaldehyde condensation products,
4% polyvinyl alcohol, 3% Arabic gum, 3% silicone oil, diatomite add to 100% parts by weight.Above-mentioned slurry carries out wet method powder with sand mill
It is broken that suspension slurry is made, spray drying granulation is then carried out, 22% fluorine azoles bacterium acyl hydroxyl is made by sieving in the particle after granulation
Amine iprodione dry suspending agent.
Example of formulations 20
Weigh 3% fluorine azoles bacterium acyl azanol, 27% iprodione, 3% fatty acid polyethylene glycol ester, 4% Negel, 4% sodium sulphate,
5% polyvinyl alcohol, 4% hexanol, precipitated calcium carbonate add to 100% parts by weight.Above-mentioned slurry is made outstanding of sand mill progress wet pulverizing
Then laitance liquid carries out spray drying granulation, 30% fluorine azoles bacterium acyl azanol iprodione is made by sieving in the particle after granulation
Dry suspending agent.
Biological Examples 1：Prevent apple marssonina leaf spot field control effectiveness test.
Example of formulations 1 has been carried out in Xianyang, Shanxi province city Jingyang County Yunyang town East Street village within 2018（22% fluorine azoles bacterium acyl hydroxyl
Amine iprodione wettable powder), example of formulations 3（22% fluorine azoles bacterium acyl azanol iprodione water dispersible granules), preparation implement
Example 5（22% fluorine azoles bacterium acyl azanol iprodione suspending agent) prevention apple marssonina leaf spot field trial, demonstrate the above example of formulations
Control effect to apple marssonina leaf spot and the safety to apple tree.
Trial crops are apple tree, and controlling object is apple marssonina leaf spot（Marssonina coronaria）.Experiment is located at
Jingyang County Yunyang town, experimental plot topography is flat, and soil is medium loam, middle fertility, pH value 7.9, during experiment in fertilizer and water management
Deng.If blank control, 4 repetitions are often handled, per 2 trees of cell, random district's groups arrangement.Using conventional spray-on process.
Investigation and statistical method：State of an illness radix is investigated before dispenser, 10d is reinvestigated once after second.Often processing cell is equal
According to tree crown top, Inner thoraxes, periphery and lower part and east, south, west, north principle, take 16 branches, fixed point is listed, by leaf point
Its whole blade of grade periodic investigation record total number of sheets, the disease numbers of sheets at different levels.Stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for 5% or less entire leaf area；
3 grades：Lesion area accounts for the 6%~15% of entire leaf area；
5 grades：Lesion area accounts for the 16%~25% of entire leaf area；
7 grades：Lesion area accounts for the 26%~50% of entire leaf area；
9 grades：Lesion area accounts for 50% or more of entire leaf area.
Disease index=[∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total leaf number × 9)] × 100
Control effect (%)=[1- (disease index after disease index × processing dispenser before control dispenser)/（The state of an illness refers to after control dispenser
Disease index before number × processing dispenser] × 100
Table 8 prevents apple marssonina leaf spot field test results
Table of field test results are bright, and the above example of formulations has good control effect to apple marssonina leaf spot, by 20g a.i./
hm2It sprays 2 times, 10 days preventive effects are above expected preventive effect after the 2nd medicine, suspend with 41.7% fluorine azoles bacterium acyl azanol of comparison medicament
Agent 9.1mg/kg, 50% iprodione wettable powder 90.9mg/kg preventive effects compare, and synergistic effect is notable.By to the 2nd medicine
10 days preventive effect significance difference analysis afterwards, embodiment chemicals treatment and comparison medicament processing preventive effect difference reach the pole level of signifiance.
Security survey to apple is observed after spray for several days after the 1st day and medicine, and each test process is to apple tree without medicine
Do harm to phenomenon.
Biological Examples 2：Prevent graw mold of tomato field control effectiveness test.
Example of formulations 7 has been carried out in Shaanxi Province Jingyang County within 2018（22% fluorine azoles bacterium acyl azanol iprodione suspension emulsion）、
Example of formulations 9（22% fluorine azoles bacterium acyl azanol iprodione aqueous emulsion）, example of formulations 11（The 22% different bacterium of fluorine azoles bacterium acyl azanol
Urea microemulsion）Graw mold of tomato field trial is prevented, demonstrates the medicament to the control effect of graw mold of tomato and to tomato
Safety.
Trial crops are tomato, and controlling object is graw mold of tomato（Botrytis cinerea）.Experiment is located at Jingyang County
Yunyang town, experimental plot topography is flat, and soil is loam, and middle fertility, pH value 6.9, fertilizer and water management is medium during experiment.If blank
Control often handles 4 repetitions, per 30 ㎡ of cell, random district's groups arrangement.Using conventional spray-on process, mu dispenser liquid 45kg uniformly sprays
It is sprinkled upon leave dual sides.
Investigation and statistical method：State of an illness radix is investigated before the 1st dispenser, 10~14d is reinvestigated after last time dispenser
Once, it investigates 2 times altogether, dispenser 2 times.Five point sampling is used per cell, investigate 3 plants, and whole blades of every plant of investigation are counted at every
Calculate disease index.Graw mold of tomato grade scale（As unit of blade）：
0 grade：Disease-free spot；
1 grade：Single blade has scab 3；
3 grades：Single blade has scab 4~6；
5 grades：Single blade has scab 7~10；
7 grades：Single blade has scab 11~20, part intensive in flakes；
9 grades：Single blade has scab is intensive to account for leaf area a quarter or more.
Disease index=[∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total leaf number × 9)] × 100
Control effect (%)=[1- (disease index after disease index × processing dispenser before control dispenser)/（The state of an illness refers to after control dispenser
Disease index before number × processing dispenser] × 100
Table 9 prevents graw mold of tomato field test results
Table of field test results are bright, and the above example of formulations has good control effect to graw mold of tomato, by 100g a.i./
hm2It sprays 2 times, 10 days preventive effects are above expected preventive effect after the 2nd medicine, suspend with 41.7% fluorine azoles bacterium acyl azanol of comparison medicament
Agent 9.1g a.i./hm2, 50% iprodione wettable powder 90.9g a.i./hm2Preventive effect compares, and synergistic effect is notable.Pass through
To 10 days preventive effect significance difference analysis after the 2nd medicine, embodiment chemicals treatment and comparison medicament processing preventive effect difference are reached and are extremely shown
It writes horizontal.
Security survey to tomato is observed after spray for several days after the 1st day and medicine, and each test process is to tomato without poisoning
Phenomenon occurs.
Biological Examples 3：Prevent powdery mildew of cucumber field trial.
Example of formulations 13 has been carried out in Shouguang City of Shandong Province within 2018（22% fluorine azoles bacterium acyl azanol iprodione micro-capsule is outstanding
Floating agent), example of formulations 15（22% fluorine azoles bacterium acyl azanol iprodione microcapsule suspension-suspendinagent agent）, example of formulations 17（22% fluorine
The dispersible oil-suspending agent of azoles bacterium acyl azanol iprodione）, example of formulations 19（22% fluorine azoles bacterium acyl azanol iprodione is dry to suspend
Agent) prevention powdery mildew of cucumber field trial, demonstrate control effect of the medicament to powdery mildew of cucumber and the safety to cucumber.
Trial crops are cucumber, and controlling object is powdery mildew of cucumber（Sphaerotheca fuliginea）.Experiment is located at Shouguang City rice
Tian Town Dong Daotiancun, experimental plot topography is flat, and soil is loam, and middle fertility, pH value 6.9, fertilizer and water management is medium during experiment.
If blank control, 4 repetitions are often handled, per 30 ㎡ of cell, random district's groups arrangement.Using conventional spray-on process, mu dispenser liquid 45kg,
Uniformly it is sprayed at leave dual sides.
Investigation and statistical method：State of an illness radix is investigated before dispenser, 10~14d is reinvestigated once after last time dispenser, altogether
Investigation 2 times.Take at random per cell at 4 points, whole blades of every 2 plants of investigation, every leaf accounts for the percentage of leaf area according to scab
Classification record.Stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for 5% or less entire leaf area；
3 grades：Lesion area accounts for the 6%~10% of entire leaf area；
5 grades：Lesion area accounts for the 11%~20% of entire leaf area；
7 grades：Lesion area accounts for the 21%~40% of entire leaf area；
9 grades：Lesion area accounts for 40% or more of entire leaf area.
Disease index=[∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total leaf number × 9)] × 100
Control effect (%)=[1- (disease index after disease index × processing dispenser before control dispenser)/（The state of an illness refers to after control dispenser
Disease index before number × processing dispenser] × 100
Table 10 prevents powdery mildew of cucumber field test results
Table of field test results are bright, and the above example of formulations has good control effect to powdery mildew of cucumber, by 100g a.i./
hm2It sprays 2 times, 10 days preventive effects are above expected preventive effect after the 2nd medicine, suspend with 41.7% fluorine azoles bacterium acyl azanol of comparison medicament
Agent 9.1g a.i./hm2, 50% iprodione wettable powder 90.9g a.i./hm2Preventive effect compares, and synergistic effect is notable.Pass through
To 10 days preventive effect significance difference analysis after the 2nd medicine, embodiment chemicals treatment and comparison medicament processing preventive effect difference are reached and are extremely shown
It writes horizontal.
Security survey to cucumber is observed after spray for several days after the 1st day and medicine, and each test process is to cucumber without poisoning
Phenomenon occurs.
In addition to apple marssonina leaf spot, graw mold of tomato and powdery mildew of cucumber, above-mentioned example of formulations is brown to citrus anthracnose, peach
Maize ear rot, sclerotinia sclerotiorum and pear scab also have synergistic effect.
Above example illustrates that the first active constituent fluorine azoles bacterium acyl azanol and the second active constituent iprodione are by weight 1：
60~60：1 compounding, can be made into wettable powder, water dispersible granules, suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspension
Agent, microcapsule suspension-suspendinagent agent, dispersible oil-suspending agent, dry suspending agent dosage form, to crop brown spot, gray mold, powdery mildew, charcoal
The fungal diseases such as subcutaneous ulcer disease, brown rot, sclerotiniose and scab have significant control effect, and synergistic effect is apparent, and dosage is few, anti-
Effect height is controlled, and good to crop safety, meets the security requirement of pesticidal preparations.
Claims (6)
1. a kind of bactericidal composition containing fluorine azoles bacterium acyl azanol and iprodione, which is characterized in that by the first active constituent fluorine azoles
The weight ratio of bacterium acyl azanol, the second active constituent iprodione and auxiliary agent composition, fluorine azoles bacterium acyl azanol and iprodione is 1：60~60：
1。
2. the bactericidal composition according to claim 1 containing fluorine azoles bacterium acyl azanol and iprodione, which is characterized in that fluorine azoles bacterium
The weight ratio of acyl azanol and iprodione is 1：30~30：1.
3. the bactericidal composition according to claim 1 containing fluorine azoles bacterium acyl azanol and iprodione, which is characterized in that fluorine azoles bacterium
The sum of content of acyl azanol and iprodione is the 1%~60% of the composition total weight.
4. the bactericidal composition according to claim 3 containing fluorine azoles bacterium acyl azanol and iprodione, which is characterized in that fluorine azoles bacterium
The sum of content of acyl azanol and iprodione is the 1%~50% of the composition total weight.
5. the bactericidal composition according to claim 1 containing fluorine azoles bacterium acyl azanol and iprodione, it is characterised in that described group
Conjunction agent type is wettable powder, water dispersible granules, suspending agent, suspension emulsion, aqueous emulsion, microemulsion, microcapsule suspending agent, micro-capsule are outstanding
Floating-suspending agent, dispersible oil-suspending agent, dry suspending agent.
6. bactericidal composition according to claim 1 containing fluorine azoles bacterium acyl azanol and iprodione prevention crop brown spot,
Purposes on gray mold, powdery mildew, anthracnose, brown rot, sclerotiniose and scab.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012041874A1 (en) * | 2010-10-01 | 2012-04-05 | Syngenta Participations Ag | Fungicidal compositions |
| CN106857540A (en) * | 2017-04-17 | 2017-06-20 | 陕西上格之路生物科学有限公司 | A kind of bactericidal composition of fluorine-containing azoles bacterium acyl azanol and triazole bactericidal agent |
| CN107006480A (en) * | 2017-04-20 | 2017-08-04 | 陕西上格之路生物科学有限公司 | A kind of bactericidal composition of fluorine-containing azoles bacterium acyl azanol |
| CN108135173A (en) * | 2015-10-09 | 2018-06-08 | 拜耳农作物科学股份公司 | Fluorine azoles bacterium acyl azanol for reducing the mycotoxin contamination of plant purposes |
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2018
- 2018-08-17 CN CN201810938738.0A patent/CN108633905A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012041874A1 (en) * | 2010-10-01 | 2012-04-05 | Syngenta Participations Ag | Fungicidal compositions |
| CN108135173A (en) * | 2015-10-09 | 2018-06-08 | 拜耳农作物科学股份公司 | Fluorine azoles bacterium acyl azanol for reducing the mycotoxin contamination of plant purposes |
| CN106857540A (en) * | 2017-04-17 | 2017-06-20 | 陕西上格之路生物科学有限公司 | A kind of bactericidal composition of fluorine-containing azoles bacterium acyl azanol and triazole bactericidal agent |
| CN107006480A (en) * | 2017-04-20 | 2017-08-04 | 陕西上格之路生物科学有限公司 | A kind of bactericidal composition of fluorine-containing azoles bacterium acyl azanol |
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Application publication date: 20181012 |