CN108606998A - It is a kind of to be used to inhibit drug of bacterium infection and preparation method thereof - Google Patents
It is a kind of to be used to inhibit drug of bacterium infection and preparation method thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
A kind of drug for inhibiting bacterium infection is composed of the following raw materials by weight：250 350 parts of honeysuckle, 250 350 parts of radix scutellariae, 550 650 parts of Fructus Forsythiae, 2.5 3.5 parts of Ribavirin.Drug provided by the invention is combined for Chinese and Western medicine, using Ribavirin, can enhance the bacteriostasis of Shuanghuanglian oral liquid, and easy to use, toxic side effect is small.
Description
Technical field
It is the present invention relates to field of medicaments, more particularly to a kind of for inhibiting drug of bacterium infection and preparation method thereof.
Background technology
Shuanghuanglian oral liquid main component has honeysuckle, radix scutellariae, Fructus Forsythiae etc., Content of Chlorogenic Acid, scutelloside, forsythin, the Chinese
Baicalein is its main active, has wider antimicrobial spectrum, there is antibacterial, antipyretic, anti-inflammatory, antiviral, dampness removing, the works such as removing jaundice
With, honeysuckle is common heat-clearing and detoxifying drug in side, for warm disease from the beginning of inducing diaphoresis, heat-clearing, removing toxic substances, stop dysentery；Radix scutellariae is common clear
Hot eliminating dampness, removing toxic substances, cool blood, purging the lung of pathogenic fire gunpowder；Fructus Forsythiae have clear away heart-fire, the heat of lung, stomach, it is evil to dissipate temperature, the effects that solving sore；Three medicines are played clearly altogether
The function of thermal detoxification, diuresis cool blood has relieving the exterior syndrome with drugs pungent in flavor and cool in property, heat-clearing toxin-expelling functions, is suitable for the upper respiratory tract caused by anemopyretic cold
The bacteriums such as infection, tonsillitis, pharyngitis, viral pneumonia and disease of viral infection, however although Shuanghuanglian oral liquid has
Antibacterial action, especially gram-positive bacteria and Gram-negative bacteria, but slowly effect, antibacterial effect are poor.
Invention content
The present invention is directed to overcome the existing technological deficiency of above-mentioned technology, it is therefore an objective to provide a kind of quick, antibacterial effect compared with
Good is used to inhibit drug of bacterium infection and preparation method thereof.
The purpose of the present invention is what is realized in the following manner：
A kind of drug for inhibiting bacterium infection is composed of the following raw materials by weight：250-350 parts of honeysuckle, radix scutellariae 250-
350 parts, 550-650 parts of Fructus Forsythiae, 2.5-3.5 parts of Ribavirin.
The preparation method of drug as described above for inhibiting bacterium infection, includes the following steps：
（1）It weighs honeysuckle, radix scutellariae, Fructus Forsythiae respectively according to quantity, crushes；
（2）Above three taste, radix scutellariae add water to cook, decocting liquid filtering, concentrate and adjusted in right amount with hydrochloric acid solution at 75-85 DEG C pH value to
1.0-2.0, keep the temperature 0.5-1.5h, stand 10-14h, filtration, precipitation plus 6-8 times measure water, with sodium hydroxide solution adjust pH value to
6.8-7.2, then add equivalent ethyl alcohol, it is stirred to dissolve, filters, filtrate hydrochloric acid solution adjusting pH value to 1.8-2.2,55-65 DEG C
20-40min is kept the temperature, 10-14h is stood, filtration, it is 6.8-7.2 that precipitation is washed till pH value with ethyl alcohol, and recycling ethyl alcohol is spare；
（3）Honeysuckle, Fructus Forsythiae are soaked, and are decocted, and decocting liquid filtration, filtrate is concentrated into the clear cream that relative density is 1.20-1.25,
Then ethyl alcohol is added, alcohol content is made to reach 70-80%, alcohol content is volume fraction, is sufficiently stirred, and stands 10-14h, filters to take
Clear liquid, recycling ethyl alcohol to no alcohol taste；
（4）Baical Skullcap root P.E and Ribavirin are added into honeysuckle, forsythia suspense extraction, with sodium hydroxide solution adjust pH value to
6.8-7.2 is stirred evenly, 4-8 DEG C refrigeration 64-80h, filtration, filtrate is filling, sterilizing to get.
Preferably, include the following steps：
（1）It weighs honeysuckle, radix scutellariae, Fructus Forsythiae respectively according to quantity, crushes；
（2）Above three taste, radix scutellariae add water to cook three times, 2 hours for the first time, each 1 hour of second and third time, collecting decoction, filtration,
Filtrate concentrates and 2mol/L hydrochloric acid solutions are added at 80 DEG C adjusts pH value to 1.0-2.0 in right amount, keeps the temperature 1 hour；It is small to stand 12
When, filtration, precipitation adds 6-8 times and measures water, adjusts pH value to 7.0 with 40% sodium hydroxide solution, then add equivalent ethyl alcohol, stirring makes molten
Solution, filtration, filtrate with 2mol/L hydrochloric acid solutions adjust pH value to 2.0,60 DEG C keep the temperature 30 minutes, stand 12 hours, filter, precipitation
It is 7.0 to be washed till pH value with ethyl alcohol, and recycling ethyl alcohol is spare；
（3）After honeysuckle, Fructus Forsythiae add water temperature to soak 30 minutes, decoction is secondary, 1.5 hours every time, collecting decoction, and filtration, filtrate exists
It is concentrated into the clear cream that relative density is 1.20-1.25 at 70-80 DEG C, ethyl alcohol is added when being cooled to 40 DEG C, makes alcohol content up to 75%, contains
Alcohol amount is volume fraction, is sufficiently stirred, and stands 12 hours, and leaching supernatant, residue adds 75% ethyl alcohol again, stirs evenly, and it is small to stand 12
When, filtration merges ethanol, recycling ethyl alcohol to no alcohol taste；
（4）Above-mentioned Baical Skullcap root P.E and Ribavirin are added into honeysuckle, forsythia suspense extraction, and adds appropriate amount of water, with 40% hydrogen
Sodium hydroxide solution adjusts pH value to 7.0, stirs evenly, and 4-8 DEG C refrigerates 72 hours, filtration, and sucrose 300g is added in filtrate, and stirring makes molten
Solution, it is appropriate and adjust pH value 1000ml is made to 7.0, Jia Shui to add essence, stirs evenly, stands 12 hours, filtration is filling, goes out
Bacterium to get.
Drug provided by the invention is combined for Chinese and Western medicine, using Ribavirin, can enhance the antibacterial work of Shuanghuanglian oral liquid
With, and it is easy to use, toxic side effect is small.
Specific implementation mode
Embodiment 1
A kind of drug for inhibiting bacterium infection is composed of the following raw materials by weight：250-350 parts of honeysuckle, radix scutellariae 250-
350 parts, 550-650 parts of Fructus Forsythiae, 2.5-3.5 parts of Ribavirin.
The preparation method of drug as described above for inhibiting bacterium infection, includes the following steps：
（1）It weighs honeysuckle, radix scutellariae, Fructus Forsythiae respectively according to quantity, crushes；
（2）Above three taste, radix scutellariae add water to cook, decocting liquid filtering, concentrate and adjusted in right amount with hydrochloric acid solution at 75-85 DEG C pH value to
1.0-2.0, keep the temperature 0.5-1.5h, stand 10-14h, filtration, precipitation plus 6-8 times measure water, with sodium hydroxide solution adjust pH value to
6.8-7.2, then add equivalent ethyl alcohol, it is stirred to dissolve, filters, filtrate hydrochloric acid solution adjusting pH value to 1.8-2.2,55-65 DEG C
20-40min is kept the temperature, 10-14h is stood, filtration, it is 6.8-7.2 that precipitation is washed till pH value with ethyl alcohol, and recycling ethyl alcohol is spare；
（3）Honeysuckle, Fructus Forsythiae are soaked, and are decocted, and decocting liquid filtration, filtrate is concentrated into the clear cream that relative density is 1.20-1.25,
Then ethyl alcohol is added, alcohol content is made to reach 70-80%, alcohol content is volume fraction, is sufficiently stirred, and stands 10-14h, filters to take
Clear liquid, recycling ethyl alcohol to no alcohol taste；
（4）Baical Skullcap root P.E and Ribavirin are added into honeysuckle, forsythia suspense extraction, with sodium hydroxide solution adjust pH value to
6.8-7.2 is stirred evenly, 4-8 DEG C refrigeration 64-80h, filtration, filtrate is filling, sterilizing to get.
Embodiment 2
A kind of drug for inhibiting bacterium infection is made of following raw material：Honeysuckle 300g, 300g parts of radix scutellariae, Fructus Forsythiae 600g,
Ribavirin 3g.
The preparation method of drug as described above for inhibiting bacterium infection, includes the following steps：
（1）It weighs honeysuckle, radix scutellariae, Fructus Forsythiae respectively according to quantity, crushes；
（2）Above three taste, radix scutellariae add water to cook three times, 2 hours for the first time, each 1 hour of second and third time, collecting decoction, filtration,
Filtrate concentrates and 2mol/L hydrochloric acid solutions are added at 80 DEG C adjusts pH value to 1.5 in right amount, keeps the temperature 1 hour；12 hours are stood, filter
It crosses, precipitation plus 7 times of amount water adjust pH value to 7.0 with 40% sodium hydroxide solution, then add equivalent ethyl alcohol, are stirred to dissolve, filter
Cross, filtrate with 2mol/L hydrochloric acid solutions adjust pH value to 2.0,60 DEG C keep the temperature 30 minutes, stand 12 hours, filtration, precipitation second
It is 7.0 that alcohol, which is washed till pH value, and recycling ethyl alcohol is spare；
（3）After honeysuckle, Fructus Forsythiae add water temperature to soak 30 minutes, decoction is secondary, 1.5 hours every time, collecting decoction, and filtration, filtrate exists
It is concentrated into the clear cream that relative density is 1.22 at 75 DEG C, ethyl alcohol is added when being cooled to 40 DEG C, makes alcohol content up to 75%, is sufficiently stirred,
12 hours are stood, leaching supernatant, residue adds appropriate 75% ethyl alcohol again, stirs evenly, and stands 12 hours, and filtration merges ethanol, returns
Ethyl alcohol is received to no alcohol taste；
（4）Above-mentioned Baical Skullcap root P.E and Ribavirin are added into honeysuckle, forsythia suspense extraction, and adds appropriate amount of water, with 40% hydrogen
Sodium hydroxide solution adjusts pH value to 7.0, stirs evenly, and 6 DEG C refrigerate 72 hours, and sucrose 300g is added in filtration, filtrate, is stirred to dissolve,
It adding essence in right amount and adjusts pH value and 1000ml is made to 7.0, Jia Shui, stir evenly, stand 12 hours, filtration is filling, sterilizing,
To obtain the final product.
Embodiment 4
A kind of drug for inhibiting bacterium infection is made of following raw material：Honeysuckle 300g, 300g parts of radix scutellariae, Fructus Forsythiae 580g,
Ribavirin 2.7g.
The preparation method of drug as described above for inhibiting bacterium infection, includes the following steps：
（1）It weighs honeysuckle, radix scutellariae, Fructus Forsythiae respectively according to quantity, crushes；
（2）Above three taste, radix scutellariae add water to cook three times, 2 hours for the first time, each 1 hour of second and third time, collecting decoction, filtration,
Filtrate concentrates and 2mol/L hydrochloric acid solutions are added at 80 DEG C adjusts pH value to 1.5 in right amount, keeps the temperature 0.5 hour；14 hours are stood,
Filtration, precipitation plus 7 times of amount water, adjust pH value to 7.2 with 40% sodium hydroxide solution, then add equivalent ethyl alcohol, are stirred to dissolve, filter
Cross, filtrate with 2mol/L hydrochloric acid solutions adjust pH value to 2.2,55 DEG C keep the temperature 30 minutes, stand 12 hours, filtration, precipitation second
It is 7 that alcohol, which is washed till pH value, and recycling ethyl alcohol is spare；
（3）After honeysuckle, Fructus Forsythiae add water temperature to soak 30 minutes, decoction is secondary, 1.5 hours every time, collecting decoction, and filtration, filtrate exists
It is concentrated into the clear cream that relative density is 1.2 at 75 DEG C, ethyl alcohol is added when being cooled to 40 DEG C, makes alcohol content up to 80%, is sufficiently stirred, it is quiet
It sets 12 hours, leaching supernatant, residue adds appropriate 75% ethyl alcohol again, stirs evenly, and stands 12 hours, filtration, merges ethanol, recycling
Ethyl alcohol is to no alcohol taste；
（4）Above-mentioned Baical Skullcap root P.E and Ribavirin are added into honeysuckle, forsythia suspense extraction, and adds appropriate amount of water, with 40% hydrogen
Sodium hydroxide solution adjusts pH value to 6.8, stirs evenly, and 6 DEG C refrigerate 72 hours, and sucrose 300g is added in filtration, filtrate, is stirred to dissolve,
It adding essence in right amount and adjusts pH value and 1000ml is made to 7.0, Jia Shui, stir evenly, stand 12 hours, filtration is filling, sterilizing,
To obtain the final product.
Embodiment 5
A kind of drug for inhibiting bacterium infection is made of following raw material：Honeysuckle 260g, 350g parts of radix scutellariae, Fructus Forsythiae 630g,
Ribavirin 3.2g.
The preparation method of drug as described above for inhibiting bacterium infection, includes the following steps：
（1）It weighs honeysuckle, radix scutellariae, Fructus Forsythiae respectively according to quantity, crushes；
（2）Above three taste, radix scutellariae add water to cook three times, 2 hours for the first time, each 1 hour of second and third time, collecting decoction, filtration,
Filtrate concentrates and 2mol/L hydrochloric acid solutions are added at 85 DEG C adjusts pH value to 1 in right amount, keeps the temperature 1.5 hours；10 hours are stood, filter
It crosses, precipitation plus 8 times of amount water adjust pH value to 6.8 with 40% sodium hydroxide solution, then add equivalent ethyl alcohol, are stirred to dissolve, filter
Cross, filtrate with 2mol/L hydrochloric acid solutions adjust pH value to 1.8,65 DEG C keep the temperature 20 minutes, stand 10 hours, filtration, precipitation second
It is 7.2 that alcohol, which is washed till pH value, and recycling ethyl alcohol is spare；
（3）After honeysuckle, Fructus Forsythiae add water temperature to soak 30 minutes, decoction is secondary, 1.5 hours every time, collecting decoction, and filtration, filtrate exists
It is concentrated into the clear cream that relative density is 1.25 at 75 DEG C, ethyl alcohol is added when being cooled to 40 DEG C, makes alcohol content up to 80%, is sufficiently stirred,
12 hours are stood, leaching supernatant, residue adds appropriate 70% ethyl alcohol again, stirs evenly, and stands 10 hours, and filtration merges ethanol, returns
Ethyl alcohol is received to no alcohol taste；
（4）Above-mentioned Baical Skullcap root P.E and Ribavirin are added into honeysuckle, forsythia suspense extraction, and adds appropriate amount of water, with 40% hydrogen
Sodium hydroxide solution adjusts pH value to 7.2, stirs evenly, and 8 DEG C refrigerate 64 hours, and sucrose 300g is added in filtration, filtrate, is stirred to dissolve,
It adding essence in right amount and adjusts pH value and 1000ml is made to 7.0, Jia Shui, stir evenly, stand 12 hours, filtration is filling, sterilizing,
To obtain the final product.
6-18 of the embodiment of the present invention is for inhibiting the pharmaceutical formulation table of bacterium infection to be shown in Table 1-2, remaining is the same as embodiment 2.
1 embodiment 6-12 formula tables of table
2 embodiment 13-18 formula tables of table
Experimental example
The recovery of 1 strain
It takes a small amount of bacterium powder to be dissolved in 1 mL sterile salines, gently blows and beats mixing.Take 50 μ l in LB meat soups with liquid-transfering gun,
37 DEG C of 120 turns/min constant-temperature tables shake 4-6h after jiggling mixing.
Count of bacteria and the preparation of dilution of bacteria
It takes 1 mL of bacterium solution of 1 preparation in 9 mL sterile purified waters with liquid-transfering gun, takes 1ml to sterilize in another 9mL after blowing and beating mixing
In distilled water, mixing is gently blown and beaten, successively doubling dilution to 10-9.10 are taken respectively-5、10-6、10-7、10-8、 10-9Extension rate
100 μ bacterium solutions of test tube on MH solid mediums, smoothened with painting bacteria stick, each sample does 3 parallel laboratory tests, 37 DEG C of cultures
18h.Bacterium number is chosen to be counted in 30~300 tablet.It chooses suitable extension rate to be diluted, bacterium solution is diluted
To 10-5Cfu/ml, 4 DEG C of refrigerations are spare.
Measurement of the swap buffers+ribavirin oral solution to Escherichia coli MIC
The dilution of 3.1 liquids
10 10 ml test tubes are taken, 2 ml sterile salines are separately added into, the swap buffers+Li Ba of 2ml is added in the first pipe
Wei Lin oral solutions take after mixing 2ml to be added in the second pipe, are discarded after being diluted to the 9th pipe successively, the control of the tenth pipe.
The preparation of MH agar mediums containing liquid
The liquid of each concentration and MH agar mediums are pressed 1：9 proportional arrangement, i.e., former each concentration liquid dilute 10 times, mix
121 °C of high pressure sterilization 30min after even, are prepared into the MH agar mediums containing each concentration liquid, 37 °C of incubators save backup.
Measurement
Take 105 The 100 μ l of bacterium solution of cfu/ml are inoculated on the MH solid mediums containing liquid, 37 °C of culture 20h,
The minimum concentration of no Escherichia coli Growth is the MIC of the liquid, tests 3 times, is averaged repeatedly.
Swap buffers+ribavirin oral solution is to the measurement of escherichia coli inhibition zone and compares
The preparation of 4.1 MH agar medium reacting holes
The bottom of culture dish is added in plain agar sterilization of liquids, places on equality testing stand after cooling, Oxford cup is placed
Face, each tablet can put 3-4, the MH agar containing Escherichia coli bacteria liquid then be added, height is substantially under the oral area of Oxford cup
Face is a little.After cooling, Oxford cup and extra solid agar are taken out with tweezers, it is spare.
The dilution of liquid
Swap buffers+ribavirin oral solution, Shuanghuanglian oral liquid is taken to be diluted ten times, 100 times with physiological saline respectively, mark
Note, 4 °C save backup.
The measurement of inhibition zone
It takes the 100 μ l of liquid of each concentration to add in reacting hole, and is marked, 37 °C of culture 20h are observed and measured and recorded it
The diameter of inhibition zone.
Experimental result
1 E. coli bacteria counts and dilution experiment result
By Escherichia coli 10 times of doubling dilutions of original bacterium solution, 100 μ l inoculations select 30~300 tablets to be counted.10-7
The bacterium number of dilution gradient is in this range：
Choose 10-7Diluted gradient is counted, and takes its average 86 to get original bacterial concentration 8.6 × 109 cfu /
Ml is diluted to 10-5Cfu/ml, it is spare.
Swap buffers+ribavirin oral solution is to Escherichia coli MIC determination experiment results
Compound shuanghuanglian oral solution shows Escherichia coli MIC experimental results, when dilution gradient is 2-6When, no Escherichia coli life
It is long, when dilution gradient is 2-7When, tablet has had a small amount of Escherichia coli Growth, and original liquor strength is 1 g/m L, i.e., such
Swap buffers+ribavirin oral solution is to the MIC of Escherichia coli between the mg of 0.78mg~1.56.
Measurement and comparative experiments result of the swap buffers+ribavirin oral solution to escherichia coli inhibition zone
By swap buffers+ribavirin oral solution, Shuanghuanglian oral liquid, 10 times, 100 times of dilution takes respectively does bacteriostatic experiment, measures
Respective inhibition zone see the table below：
Test brief summary
This experiment is mainly swap buffers+ribavirin oral solution to standard strain Escherichia coli（25922）The measurement of MIC value,
MIC value is between 1.95mg~3.90mg and its measurement of inhibition zone size is compared with Shuanghuanglian oral liquid, inhibition zone
It is even to be better than the latter.
What has been described above is only a preferred embodiment of the present invention, it is noted that for those skilled in the art,
Under the premise of not departing from general idea of the present invention, several changes and improvements can also be made, these should also be considered as the present invention's
Protection domain.
Claims (3)
1. a kind of drug for inhibiting bacterium infection, it is characterised in that be composed of the following raw materials by weight：Honeysuckle 250-350
Part, 250-350 parts of radix scutellariae, 550-650 parts of Fructus Forsythiae, 2.5-3.5 parts of Ribavirin.
2. the preparation method of the drug as described in claim 1 for inhibiting bacterium infection, it is characterised in that including following step
Suddenly：
（1）It weighs honeysuckle, radix scutellariae, Fructus Forsythiae respectively according to quantity, crushes；
（2）Above three taste, radix scutellariae add water to cook, decocting liquid filtering, concentrate and adjusted in right amount with hydrochloric acid solution at 75-85 DEG C pH value to
1.0-2.0, keep the temperature 0.5-1.5h, stand 10-14h, filtration, precipitation plus 6-8 times measure water, with sodium hydroxide solution adjust pH value to
6.8-7.2, then add equivalent ethyl alcohol, it is stirred to dissolve, filters, filtrate hydrochloric acid solution adjusting pH value to 1.8-2.2,55-65 DEG C
20-40min is kept the temperature, 10-14h is stood, filtration, it is 6.8-7.2 that precipitation is washed till pH value with ethyl alcohol, and recycling ethyl alcohol is spare；
（3）Honeysuckle, Fructus Forsythiae are soaked, and are decocted, and decocting liquid filtration, filtrate is concentrated into the clear cream that relative density is 1.20-1.25,
Then ethyl alcohol is added, alcohol content is made to reach 70-80%, alcohol content is volume fraction, is sufficiently stirred, and stands 10-14h, filters to take
Clear liquid, recycling ethyl alcohol to no alcohol taste；
（4）Baical Skullcap root P.E and Ribavirin are added into honeysuckle, forsythia suspense extraction, with sodium hydroxide solution adjust pH value to
6.8-7.2 is stirred evenly, 4-8 DEG C refrigeration 64-80h, filtration, filtrate is filling, sterilizing to get.
3. the preparation method of the drug as claimed in claim 2 for inhibiting bacterium infection, it is characterised in that including following step
Suddenly：
（1）It weighs honeysuckle, radix scutellariae, Fructus Forsythiae respectively according to quantity, crushes；
（2）Above three taste, radix scutellariae add water to cook three times, 2 hours for the first time, each 1 hour of second and third time, collecting decoction, filtration,
Filtrate concentrates and 2mol/L hydrochloric acid solutions are added at 80 DEG C adjusts pH value to 1.0-2.0 in right amount, keeps the temperature 1 hour；It is small to stand 12
When, filtration, precipitation adds 6-8 times and measures water, adjusts pH value to 7.0 with 40% sodium hydroxide solution, then add equivalent ethyl alcohol, stirring makes molten
Solution, filtration, filtrate with 2mol/L hydrochloric acid solutions adjust pH value to 2.0,60 DEG C keep the temperature 30 minutes, stand 12 hours, filter, precipitation
It is 7.0 to be washed till pH value with ethyl alcohol, and recycling ethyl alcohol is spare；
（3）After honeysuckle, Fructus Forsythiae add water temperature to soak 30 minutes, decoction is secondary, 1.5 hours every time, collecting decoction, and filtration, filtrate exists
It is concentrated into the clear cream that relative density is 1.20-1.25 at 70-80 DEG C, ethyl alcohol is added when being cooled to 40 DEG C, makes alcohol content up to 75%, contains
Alcohol amount is volume fraction, is sufficiently stirred, and stands 12 hours, and leaching supernatant, residue adds 75% ethyl alcohol again, stirs evenly, and it is small to stand 12
When, filtration merges ethanol, recycling ethyl alcohol to no alcohol taste；
（4）Above-mentioned Baical Skullcap root P.E and Ribavirin are added into honeysuckle, forsythia suspense extraction, and adds appropriate amount of water, with 40% hydrogen
Sodium hydroxide solution adjusts pH value to 7.0, stirs evenly, and 4-8 DEG C refrigerates 72 hours, filtration, and sucrose 300g is added in filtrate, and stirring makes molten
Solution, it is appropriate and adjust pH value 1000ml is made to 7.0, Jia Shui to add essence, stirs evenly, stands 12 hours, filtration is filling, goes out
Bacterium to get.
Priority Applications (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112618561A (en) * | 2021-02-25 | 2021-04-09 | 成都大学 | Application of ribavirin in preparation of medicine for inhibiting bacterial quorum sensing |
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| CN1899381A (en) * | 2006-07-13 | 2007-01-24 | 天津中天制药有限公司 | Shuanghuangli dripping pill and its preparing method |
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| CN112618561A (en) * | 2021-02-25 | 2021-04-09 | 成都大学 | Application of ribavirin in preparation of medicine for inhibiting bacterial quorum sensing |
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