CN108576028A - A kind of bactericidal composition of prevention and control cucurbita pepo soft rot - Google Patents
A kind of bactericidal composition of prevention and control cucurbita pepo soft rot Download PDFInfo
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- CN108576028A CN108576028A CN201810511899.1A CN201810511899A CN108576028A CN 108576028 A CN108576028 A CN 108576028A CN 201810511899 A CN201810511899 A CN 201810511899A CN 108576028 A CN108576028 A CN 108576028A
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- bactericidal composition
- jervine
- zhongshengmycin
- graphene oxide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
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- Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to plant disease Control Technology fields, disclose a kind of bactericidal composition of prevention and control cucurbita pepo soft rot, include each component of following weight percent：Jervine 12~25%, Zhongshengmycin 5~18%, graphene oxide 0.1~0.8%, surplus auxiliary agent；The dosage form of the bactericidal composition is any one of wettable powder, suspending agent or water dispersible granules.For the present invention by the way that graphene oxide and jervine, Zhongshengmycin to be used in compounding, three belongs to the fungicide of different role mechanism, and mutual mixture not will produce conflict；There is good synergistic function, preventive effect to be higher than single dose in a certain range, drug effect is more longlasting, substantially increases germicidal efficiency, has good control effect to cucurbita pepo soft rot；Pathogen will not be made to develop immunity to drugs, expand fungicidal spectrum, reduced dosage and cost, can be used for the prevention of agricultural production crop disease.
Description
Technical field
The present invention relates to plant disease Control Technology fields, and in particular, to a kind of sterilization of prevention and control cucurbita pepo soft rot
Composition.
Background technology
Cucurbita pepo soft rot is mainly by Erwinia carotovora subsp.carotovora (Erwinia carotovora
Subsp.carotovora, E.c.c) caused by bacterial disease.In recent years, the winter warm type on China Shandong, Hebei and other places is big
Occur to be in exacerbation trend in canopy, the basal part of stem of main harm cucurbita pepo, coverage is more and more wider, it has also become endangers winter protection
Ground cucurbita pepo Major Diseases.The prevention of cucurbita pepo soft rot is relied primarily on using chemical agent at present, and chemical agent is of high cost, is changed
Learn medicament it is difficult to degrade, to plant itself have damage or it is toxic, can also bring environmental pollution, cucurbita pepo pollution of agricultural products etc. to ask
Topic.In addition, not finding to be directed to the particularly efficient chemical agent of cucurbita pepo soft rot at present yet.
Black false hellebore is Liliaceae Veratrum, is herbaceos perennial, and jervine is present in Liliaceae Veratrum and spray
It sneezes in careless platymiscium, raw material is the rhizome of the seed and white black false hellebore, RADIX ET RHIZOMA VERATRI NIGRI of sabadilla.Plant material is extracted or carried through ethyl alcohol
It takes obtained.Correlative study shows jervine to plant disease such as brown rot, gray mold, soft rot, scab, bacillary perforation
Disease, downy mildew, phytophthora root rot, powdery mildew, late blight, dry rot have good inhibiting effect.
Zhongshengmycin belongs to N- glycoside antibiotic, and antimicrobial spectrum is very wide, can resist Gram-positive, negative bacteria well,
Multiple diseases caused by mycobacteria, saccharomycete and filamentous fungi.To Bacteria erwinia, angular leaf spot fungus, leaf spot bacteria, rice blast
Bacterium, Target spot pathogen, gibberellic hypha etc. all have apparent antibacterial activity.Its mechanism of action is：By inhibiting microorganism albumen
Matter synthesizes and keeps microorganism dead；Made by making der Pilz deformation of filament, inhibiting spore germination or directly killing the modes such as spore
For filamentous fungi；The generation that the precursor substance of protective plant protecting agent and lignin in plant can be stimulated, to improve the disease-resistant of plant
Ability increases income conducive to crop yield.
Graphene oxide (GO) is a kind of two dimensional surface material of monoatomic layer, has prodigious specific surface area.Due to GO
There are many oxygen-containing groups on surface, so GO also has good hydrophily.The sterilization mechanism of GO materials is substantially divided at present
For following 3 kinds of mainstream opinions：The edge of nano material damaging cells film as blade leads to intracellular DNA, RNA and thin
Born of the same parents' device etc. leaks into extracellularly, leads to cell death；The oxidative pressure that material generates can be with the enzyme and albumen of oxidizing bacteria cell
DNA can be oxidizing to and untwist and be broken, while can also aoxidize mitochondria by matter, and energy supply is made to go wrong；Nano flake packet
Cell membrane is wrapped up in and wound, bacterium is come out with ambient environment, some important channels blocked on cell membrane allow cell cannot
Energy exchange is generated with surrounding.
On the one hand, jervine and Zhongshengmycin all have higher bactericidal activity, if but long-term exclusive use, it is easy to make
Plant germ develops immunity to drugs, and then generates the problems such as dosage increases, preventive effect reduces, the lasting period shortens.Meanwhile pesticide is anti-
Multiple use will also result in the degradation rate decline of pesticide in the soil, be unfavorable for very much environment sustainable development.On the other hand, stone is aoxidized
Although black alkene bactericidal effect is fine, long-term be used alone can cause normal plant cells a degree of damage.It will be different
The active ingredient of the mechanism of action is reasonably compounded, and is that the damage that delays disease to develop immunity to drugs and reduce to host plant is non-
Normal effective common method, and can be according to the effect in production application, to judge that this compounding is synergistic effect or short of money
Anti- effect.But for jervine, Zhongshengmycin and the compounded combination of graphene oxide three, there has been no excessively related reports at present
Road.
Invention content
The purpose of the invention is to overcome the above-mentioned deficiency of the prior art, a kind of killing for prevention and control cucurbita pepo soft rot is provided
Bacteria composition, for the present invention by the way that graphene oxide and jervine, Zhongshengmycin to be used in compounding, three belongs to different role mechanism
Fungicide, mutual mixture not will produce conflict；There is good synergistic function in a certain range, preventive effect is higher than single
Agent, drug effect are more longlasting.
To achieve the goals above, the present invention is achieved by following scheme：
A kind of bactericidal composition of prevention and control cucurbita pepo soft rot, includes each component of following weight percent：Jervine 12
~25%, Zhongshengmycin 5~18%, graphene oxide 0.1~0.8%, surplus auxiliary agent；The dosage form of the bactericidal composition is can
Any one of wet powder, suspending agent or water dispersible granules.
Three kinds of ingredient mechanism of action points are different in the present composition, and the present inventor passes through a large number of experiments recipe determination, carries
Gone out a kind of Compositional type bactericidal composition of the present invention, i.e., using jervine, Zhongshengmycin and graphene oxide as active ingredient, into
Capable Recompounded multielement bactericidal composition.Since the mechanism of action point of these three ingredients is different, by jervine, Zhongshengmycin and oxidation
Graphene compounds in certain proportion, has good synergy synergistic effect, substantially increases germicidal efficiency, and can prolong well
The generation of slow Disease Resistance, expands fungicidal spectrum, reduces drug cost, improves practical preventive effect, can be used for preventing cucurbita pepo soft rot.
Preferably, when bactericidal composition is wettable powder, include each component of following weight percent：Jervine 15
~20%, Zhongshengmycin 11~16%, graphene oxide 0.1~0.8%, dispersant 1~10%, wetting agent 1~10%, filler
1~25%.Jervine, Zhongshengmycin, graphene oxide and dispersant, wetting agent and filler are mixed by above-mentioned weight proportion
It closes, in a stirring kettle uniform stirring, remixes after being smashed by air jet mill uniformly, obtain wettable powder bactericidal composition.
Preferably, when bactericidal composition is suspending agent, include each component of following weight percent：Jervine 12~
16%, Zhongshengmycin 8~15%, graphene oxide 0.1~0.8%, dispersant 5~10%, antifreezing agent 1~5%, thickener
0.1~1%, antifoaming agent 0.1~0.8%, excess water.The specific production stage of the suspending agent is：First auxiliary agent is matched by above-mentioned
Than mixing, be uniformly mixed through high speed shear, add jervine, Zhongshengmycin and graphene oxide, in ball crusher abrading-ball 2~
3 hours, a diameter is made to be below 5mm, obtains the bactericidal composition of aqueous suspension formulations.
Preferably, when bactericidal composition is water dispersible granules, include each component of following weight percent：Jervine 15
~25%, Zhongshengmycin 8~15%, graphene oxide 0.1~0.8%, dispersant 5~10%, wetting agent 4~10%, disintegration
Agent 1~5%, filler 1~25%.The specific production stage of the aqueous emulsion is：By jervine, Zhongshengmycin, graphene oxide, divide
Powder, wetting agent, disintegrant and filler are uniformly mixed, and are crushed with micro jet, and through mediating, fluid bed is then added
It is granulated, dried, sieved in granulating and drying machine, obtain the bactericidal composition of water dispersant.
Preferably, the dispersant is aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, fatty acid glyceride gather
At least one of ethylene oxide ether, poloxamer.
Preferably, the wetting agent is in lauryl sodium sulfate, calcium dodecyl benzene sulfonate, alkylnaphthalene sulfonate
It is at least one.
Preferably, the filler is at least one of talcum powder, kaolin, diatomite, bentonite.
Preferably, the antifreezing agent is at least one of ethylene glycol, propylene glycol, glycerine, polyethylene glycol；Described
Thickener be xanthans, hydroxymethyl cellulose, hydroxyethyl cellulose, methylcellulose, aluminum magnesium silicate, in polyvinyl alcohol at least
It is a kind of；The antifoaming agent is at least one of sodium citrate, resorcinol.
Preferably, the disintegrant is at least one of urea, ammonium sulfate, aluminium chloride, citric acid, sodium bicarbonate.
Compared with prior art, the invention has the advantages that：
For the present invention by the way that graphene oxide and jervine, Zhongshengmycin to be used in compounding, three belongs to different role mechanism
Fungicide, mutual mixture not will produce conflict；There is good synergistic function in a certain range, preventive effect is higher than single
Agent, drug effect is more longlasting, substantially increases germicidal efficiency, has good control effect to cucurbita pepo soft rot；It will not make pathogen
It develops immunity to drugs, expands fungicidal spectrum, reduce dosage and cost, can be used for the prevention of agricultural production crop disease.
Specific implementation mode
The present invention is made with reference to specific embodiment and further being elaborated, the embodiment is served only for explaining this
Invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is normal unless otherwise specified
Rule method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
The incidence that whole strain blade is investigated according to experiment grade scale, calculates disease index and control effect.According to grandson
Yun Peifa (《The Simple Calculation of co-toxicity coefficient and its application》, Huang attain clearly, 1993) calculate medicament toxicity index grade altogether poison be
Number (CTC).CTC≤80, composition show as antagonism；80<CTC<120, composition shows as summation action；CTC≥
120, composition shows as synergistic effect.
1 30% jervine Zhongshengmycin graphene oxide wettable powder of embodiment
By 15% jervine, 14.6% Zhongshengmycin, 0.4% graphene oxide, 8% poloxamer, 9% dodecyl sulphur
Sour sodium and the mixing of 20% bentonite, uniform stirring, remixes uniformly, is made 30% after being smashed by air jet mill in a stirring kettle
Jervine Zhongshengmycin graphene oxide wettable powder.
2 30% jervine Zhongshengmycin graphene oxide wettable powder of embodiment
By 18% jervine, 11.2% Zhongshengmycin, 0.8% graphene oxide, 8% aliphatic amine polyoxyethylene ether, 8.5%
Calcium dodecyl benzene sulfonate, 15% diatomite, the mixing of 6% bentonite, uniform stirring, crushes through airslide disintegrating mill in a stirring kettle
After remix uniformly, be made 30% jervine Zhongshengmycin graphene oxide wettable powder.
3 25% jervine Zhongshengmycin graphene oxide suspending agent of embodiment
By 7% polyoxyethylene carboxylate, 3% propylene glycol, 0.3% hydroxyethyl cellulose, 0.4% sodium citrate and
64.3% water mixes, and after being uniformly mixed through high speed shear, adds 13% jervine, 11.4% Zhongshengmycin and 0.6% oxidation
Graphene, abrading-ball 2~3 hours in ball crusher, makes a diameter be below 5mm, and 25% jervine Zhongshengmycin is made
Graphene oxide suspending agent.
4 25% jervine Zhongshengmycin graphene oxide suspending agent of embodiment
10% poloxamer, 2% glycerine, 1.0% xanthans, 0.5% sodium citrate and 61.5% water are mixed, through height
After fast shear-mixed is uniform, 15% jervine, 9.7% Zhongshengmycin and 0.3% graphene oxide are added, is ground in ball crusher
Ball 2~3 hours, makes a diameter be below 5mm, and 25% jervine Zhongshengmycin graphene oxide suspending agent is made.
5 25% jervine Zhongshengmycin graphene oxide water dispersible granules of embodiment
By 16% jervine, 8.3% Zhongshengmycin, 0.7% graphene oxide, 7.8% aliphatic amine polyoxyethylene ether, 4%
Lauryl sodium sulfate, 5% calcium dodecyl benzene sulfonate, 2.5% sodium bicarbonate, 20% kaolin are uniformly mixed, with ultra micro gas
Flow pulverizer crush, through mediate, then be added fluidized-bed granulation dryer in be granulated, dry, sieve after sampling analysis, make
Obtain 25% jervine Zhongshengmycin graphene oxide water dispersible granules.
6 25% jervine Zhongshengmycin graphene oxide water dispersible granules of embodiment
By 15.2% jervine, 9% Zhongshengmycin, 0.8% graphene oxide, 5% glycerol fatty acid ester polyoxyethylene ethe,
6% calcium dodecyl benzene sulfonate, 4.5% ammonium sulfate, 22% diatomite are uniformly mixed, and are crushed with micro jet, through pinching
Close, be then added in fluidized-bed granulation dryer be granulated, dried, sieve after sampling analysis, in obtained 25% jervine
Raw rhzomorph graphene oxide water dispersible granules.
Bacteriostatic activity of the 7 Examples 1 to 6 bactericidal composition of embodiment to cucurbita pepo Bacteria erwinia
Bacteriostatic activity of the Examples 1 to 6 bactericidal composition to cucurbita pepo Bacteria erwinia is measured using micro-dilution method.Step
It is as follows：
It will be in examination bacterium access Mueller-Hinton broth bouillons, 48h being cultivated in 35 DEG C, until turning after slight haziness
Enter in 0.9% physiological saline, adjusts that (assay method is to make it under 650nm wavelength using spectrophotometer to Maxwell turbidity 0.5
Absorbance be 0.020), then with Mueller-Hinton broth bouillons dilute 200 times it is spare.The bacterium solution diluted is connected to 96
In orifice plate, per 100 μ L of hole liquid volume added, then it is separately added into Examples 1 to 6 bactericidal composition, using streptomycin sulphate as positive control,
Not connect the processing of bacterium as negative control with bacteria culture fluid and dosing (Examples 1 to 6 bactericidal composition).96 holes that will be handled well
Plate is placed in 35 DEG C of incubators and cultivates 20h, and light transmittance is measured under 650nm wavelength, records Examples 1 to 6 bactericidal composition pair
For trying the inhibition growth minimum concentration (MIC) of bacterium, it is the standard for inhibiting growth to be more than 85% with light transmittance, the results are shown in Table 1.
Minimum inhibitory concentration (MIC) measurement result of 1 Examples 1 to 6 bactericidal composition of table to cucurbita pepo Bacteria erwinia
| Processing | Minimum inhibitory concentration MIC (μ g/mL) |
| Embodiment 1 | 23 |
| Embodiment 2 | 30 |
| Embodiment 3 | 45 |
| Embodiment 4 | 40 |
| Embodiment 5 | 33 |
| Embodiment 6 | 39 |
| Streptomycin sulphate | 117 |
The result shows that 1~6 bactericidal composition of the embodiment of the present invention is apparently higher than the bacteriostatic activity of cucurbita pepo Bacteria erwinia
Streptomycin sulphate.
The toxicity test of 8 jervine of embodiment, Zhongshengmycin and graphene oxide to cucurbita pepo Bacteria erwinia
Jervine, Zhongshengmycin and graphene oxide compounding test cucurbita pepo Bacteria erwinia toxicity test, specific to match
With the results are shown in Table 2~3.
2 jervine of table, Zhongshengmycin, graphene oxide single dose are to cucurbita pepo Bacteria erwinia survey toxicity index
| Processing | LD50(μg/mL) | TI |
| Jervine | 0.93 | 100 |
| Zhongshengmycin | 2.55 | 36.47 |
| Graphene oxide | 21.24 | 4.38 |
3 jervine of table, Zhongshengmycin and graphene oxide different proportion compounding are to cucurbita pepo Bacteria erwinia toxicity test knot
Fruit is analyzed
The result shows that jervine, Zhongshengmycin and graphene oxide significantly improve the control effect of cucurbita pepo soft rot,
Three's compound proportion has a significant synergistic effect to cucurbita pepo Bacteria erwinia within the scope of the present invention, co-toxicity coefficient 150 with
On, synergistic effect is apparent；And three's compound proportion is not within the scope of the present invention, to cucurbita pepo Bacteria erwinia can only achieve addition
Effect, co-toxicity coefficient are less than 120.
9 field efficacy confirmatory experiment of embodiment
Test method：It is once sprayed at the initial stage of a disease, each handles 3 cells, each cell 20m2.Before medicine and
10 days investigation statistics incidences after medicine, 5 grab sample of each cell investigate the lesion area in whole strain per blade and account for blade
The percentage of area calculates disease index and control effect.Every group of pharmaceutical quantities sprayed are with active constituent content in composition
It is all mutually foundation.
Field experiment is carried out to cucurbita pepo soft rot using Examples 1 to 6 bactericidal composition, the results are shown in Table 4：
4 cucurbita pepo soft rot disease index of table and control effect
The result shows that jervine, Zhongshengmycin and graphene oxide compounding are notable to the control effect of cucurbita pepo soft rot.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of shield range can also be made on the basis of above description and thinking for those of ordinary skill in the art
Other various forms of variations or variation, there is no necessity and possibility to exhaust all the enbodiments.It is all the present invention
All any modification, equivalent and improvement etc., should be included in the protection of the claims in the present invention made by within spirit and principle
Within the scope of.
Claims (9)
1. a kind of bactericidal composition of prevention and control cucurbita pepo soft rot, which is characterized in that include each component of following weight percent：
Jervine 12~25%, Zhongshengmycin 5~18%, graphene oxide 0.1~0.8%, surplus auxiliary agent；The agent of the bactericidal composition
Type is any one of wettable powder, suspending agent or water dispersible granules.
2. bactericidal composition according to claim 1, which is characterized in that when bactericidal composition is wettable powder, packet
Include each component of following weight percent：Jervine 15~20%, Zhongshengmycin 11~16%, graphene oxide 0.1~0.8%, point
Powder 1~10%, wetting agent 1~10%, filler 1~25%.
3. bactericidal composition according to claim 1, which is characterized in that when bactericidal composition is suspending agent, including with
The each component of lower weight percent：Jervine 12~16%, Zhongshengmycin 8~15%, graphene oxide 0.1~0.8%, dispersant 5
~10%, antifreezing agent 1~5%, thickener 0.1~1%, antifoaming agent 0.1~0.8%, excess water.
4. bactericidal composition according to claim 1, which is characterized in that when bactericidal composition is water dispersible granules, packet
Include each component of following weight percent：Jervine 15~25%, Zhongshengmycin 8~15%, graphene oxide 0.1~0.8%, point
Powder 5~10%, wetting agent 4~10%, disintegrant 1~5%, filler 1~25%.
5. according to claim 2~4 any one of them bactericidal composition, which is characterized in that the dispersant is fatty amine
At least one of polyoxyethylene ether, polyoxyethylene carboxylate, glycerol fatty acid ester polyoxyethylene ethe, poloxamer.
6. according to any bactericidal composition of claim 2 or 4, which is characterized in that the wetting agent is dodecyl
At least one of sodium sulphate, calcium dodecyl benzene sulfonate, alkylnaphthalene sulfonate.
7. according to any bactericidal composition of claim 2 or 4, which is characterized in that the filler is talcum powder, kaolinite
At least one of soil, diatomite, bentonite.
8. bactericidal composition according to claim 3, which is characterized in that the antifreezing agent is ethylene glycol, propylene glycol, third
At least one of triol, polyethylene glycol；The thickener is xanthans, hydroxymethyl cellulose, hydroxyethyl cellulose, methyl
At least one of cellulose, aluminum magnesium silicate, polyvinyl alcohol；The antifoaming agent is sodium citrate, in resorcinol at least
It is a kind of.
9. bactericidal composition according to claim 4, which is characterized in that the disintegrant is urea, ammonium sulfate, chlorination
At least one of aluminium, citric acid, sodium bicarbonate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810511899.1A CN108576028A (en) | 2018-05-24 | 2018-05-24 | A kind of bactericidal composition of prevention and control cucurbita pepo soft rot |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810511899.1A CN108576028A (en) | 2018-05-24 | 2018-05-24 | A kind of bactericidal composition of prevention and control cucurbita pepo soft rot |
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ID=63629533
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