CN108402077A - A kind of bactericidal composition of prevention and control soft rot of Chinese cabbage - Google Patents
A kind of bactericidal composition of prevention and control soft rot of Chinese cabbage Download PDFInfo
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- CN108402077A CN108402077A CN201810511700.5A CN201810511700A CN108402077A CN 108402077 A CN108402077 A CN 108402077A CN 201810511700 A CN201810511700 A CN 201810511700A CN 108402077 A CN108402077 A CN 108402077A
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- bactericidal composition
- jervine
- zhongshengmycin
- graphene oxide
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- 239000000203 mixture Substances 0.000 title claims abstract description 47
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 38
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 title claims abstract description 21
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 title claims abstract description 21
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 title claims abstract description 21
- 230000002265 prevention Effects 0.000 title claims abstract description 9
- CLEXYFLHGFJONT-DNMILWOZSA-N Jervine Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H](C(=O)C2=C3C)[C@@H]1[C@@H]2CC[C@@]13O[C@@H]2C[C@H](C)CN[C@H]2[C@H]1C CLEXYFLHGFJONT-DNMILWOZSA-N 0.000 claims abstract description 46
- CLEXYFLHGFJONT-UHFFFAOYSA-N jervine Natural products C1C=C2CC(O)CCC2(C)C(C(=O)C2=C3C)C1C2CCC13OC2CC(C)CNC2C1C CLEXYFLHGFJONT-UHFFFAOYSA-N 0.000 claims abstract description 46
- QRXOCOSLDOPPKH-UHFFFAOYSA-N jervine sulfate Natural products CC1CNC2C(C1)OC3(CCC4=C(C3C)C(=O)C5C4CC=C6CC(O)CCC56C)C2C QRXOCOSLDOPPKH-UHFFFAOYSA-N 0.000 claims abstract description 46
- VMFXMTJCTSYHCF-HHQUSWFZSA-N [(2r,3r,4s,5r)-5-(hexylamino)-4-hydroxy-2-(hydroxymethyl)-6-[(7-hydroxy-4-oxo-1,3a,5,6,7,7a-hexahydroimidazo[4,5-c]pyridin-2-yl)amino]oxan-3-yl] carbamate Chemical compound CCCCCCN[C@@H]1[C@H](O)[C@@H](OC(N)=O)[C@@H](CO)OC1\N=C\1NC(C(=O)NCC2O)C2N/1 VMFXMTJCTSYHCF-HHQUSWFZSA-N 0.000 claims abstract description 44
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 42
- 229910021389 graphene Inorganic materials 0.000 claims abstract description 41
- 239000000375 suspending agent Substances 0.000 claims abstract description 10
- 239000004562 water dispersible granule Substances 0.000 claims abstract description 9
- 239000004563 wettable powder Substances 0.000 claims abstract description 9
- 239000012752 auxiliary agent Substances 0.000 claims abstract description 4
- 239000002552 dosage form Substances 0.000 claims abstract description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerol Natural products OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 8
- 239000002270 dispersing agent Substances 0.000 claims description 8
- 239000000945 filler Substances 0.000 claims description 8
- -1 polyoxyethylene Polymers 0.000 claims description 8
- 239000000080 wetting agent Substances 0.000 claims description 8
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical group OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 claims description 5
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 5
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 5
- 229960000502 poloxamer Drugs 0.000 claims description 5
- 229920001983 poloxamer Polymers 0.000 claims description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- 239000002518 antifoaming agent Substances 0.000 claims description 4
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 claims description 4
- 239000007884 disintegrant Substances 0.000 claims description 4
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 claims description 4
- 239000001509 sodium citrate Substances 0.000 claims description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical group O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 4
- 239000002562 thickening agent Substances 0.000 claims description 4
- 239000002202 Polyethylene glycol Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 239000000440 bentonite Substances 0.000 claims description 3
- 229910000278 bentonite Inorganic materials 0.000 claims description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 150000007942 carboxylates Chemical class 0.000 claims description 3
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 3
- 239000000194 fatty acid Substances 0.000 claims description 3
- 229930195729 fatty acid Natural products 0.000 claims description 3
- 229920003063 hydroxymethyl cellulose Polymers 0.000 claims description 3
- 229940031574 hydroxymethyl cellulose Drugs 0.000 claims description 3
- 229920001223 polyethylene glycol Polymers 0.000 claims description 3
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 3
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 3
- 229920001285 xanthan gum Polymers 0.000 claims description 3
- 239000004354 Hydroxyethyl cellulose Substances 0.000 claims description 2
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 claims description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 2
- 229940009868 aluminum magnesium silicate Drugs 0.000 claims description 2
- WMGSQTMJHBYJMQ-UHFFFAOYSA-N aluminum;magnesium;silicate Chemical compound [Mg+2].[Al+3].[O-][Si]([O-])([O-])[O-] WMGSQTMJHBYJMQ-UHFFFAOYSA-N 0.000 claims description 2
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical group O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 2
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 claims description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 239000002689 soil Substances 0.000 claims description 2
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims 2
- 239000006185 dispersion Substances 0.000 claims 2
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 claims 1
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical compound ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 claims 1
- 239000004411 aluminium Substances 0.000 claims 1
- 229910052782 aluminium Inorganic materials 0.000 claims 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims 1
- 150000001412 amines Chemical class 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 claims 1
- 229910052622 kaolinite Inorganic materials 0.000 claims 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 1
- 229910052938 sodium sulfate Inorganic materials 0.000 claims 1
- 235000011152 sodium sulphate Nutrition 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 11
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- 239000003814 drug Substances 0.000 abstract description 8
- 238000013329 compounding Methods 0.000 abstract description 7
- 230000002195 synergetic effect Effects 0.000 abstract description 7
- 230000000855 fungicidal effect Effects 0.000 abstract description 6
- 229940079593 drug Drugs 0.000 abstract description 5
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- 230000000857 drug effect Effects 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
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- 230000002070 germicidal effect Effects 0.000 abstract description 3
- 230000005923 long-lasting effect Effects 0.000 abstract description 3
- 244000052769 pathogen Species 0.000 abstract description 3
- 230000001717 pathogenic effect Effects 0.000 abstract description 3
- 238000012271 agricultural production Methods 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 15
- 241000588698 Erwinia Species 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 230000002401 inhibitory effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 239000005995 Aluminium silicate Substances 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 241000489523 Veratrum Species 0.000 description 4
- 235000012211 aluminium silicate Nutrition 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
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- 230000010534 mechanism of action Effects 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
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- 230000001590 oxidative effect Effects 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- QTENRWWVYAAPBI-YCRXJPFRSA-N streptomycin sulfate Chemical compound OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](N=C(N)N)[C@H](O)[C@@H](N=C(N)N)[C@H](O)[C@H]1O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](N=C(N)N)[C@H](O)[C@@H](N=C(N)N)[C@H](O)[C@H]1O QTENRWWVYAAPBI-YCRXJPFRSA-N 0.000 description 3
- 231100000820 toxicity test Toxicity 0.000 description 3
- RZYKUPXRYIOEME-UHFFFAOYSA-N CCCCCCCCCCCC[S] Chemical compound CCCCCCCCCCCC[S] RZYKUPXRYIOEME-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 241000234280 Liliaceae Species 0.000 description 2
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- 239000004480 active ingredient Substances 0.000 description 2
- 150000001336 alkenes Chemical class 0.000 description 2
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
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- 150000001875 compounds Chemical class 0.000 description 2
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical group OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 2
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- 235000013311 vegetables Nutrition 0.000 description 2
- 229940057613 veratrum Drugs 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
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- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
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- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 1
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- 241000588701 Pectobacterium carotovorum Species 0.000 description 1
- 241000233679 Peronosporaceae Species 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
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- 230000003115 biocidal effect Effects 0.000 description 1
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- 238000004364 calculation method Methods 0.000 description 1
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- 230000006872 improvement Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Inorganic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to plant disease Control Technology fields, disclose a kind of bactericidal composition of prevention and control soft rot of Chinese cabbage, include each component of following weight percent：Jervine 5~20%, Zhongshengmycin 5~10%, graphene oxide 0.1~0.5%, surplus auxiliary agent；The dosage form of the bactericidal composition is any one of wettable powder, suspending agent or water dispersible granules.For the present invention by the way that graphene oxide and jervine, Zhongshengmycin to be used in compounding, three belongs to the fungicide of different role mechanism, and mutual mixture not will produce conflict；There is good synergistic function, preventive effect to be higher than single dose in a certain range, drug effect is more longlasting, substantially increases germicidal efficiency, has good control effect to soft rot of Chinese cabbage；Pathogen will not be made to develop immunity to drugs, expand fungicidal spectrum, reduced dosage and cost, can be used for the prevention of agricultural production crop disease.
Description
Technical field
The present invention relates to plant disease Control Technology fields, and in particular, to a kind of sterilization of prevention and control soft rot of Chinese cabbage
Composition.
Background technology
Soft rot of Chinese cabbage is the worldwide prevalence caused by Erwinia (Erwinia carotovora)
Disease, at home various regions generally occur, be one of big important disease of China Chinese cabbage three, the quality to China vegetables and vegetable grower's warp
It helps and causes serious influence, common chemical pesticide control disease in production.
Black false hellebore is Liliaceae Veratrum, is herbaceos perennial, and jervine is present in Liliaceae Veratrum and spray
It sneezes in careless platymiscium, raw material is the rhizome of the seed and white black false hellebore, RADIX ET RHIZOMA VERATRI NIGRI of sabadilla.Plant material is extracted or carried through ethyl alcohol
It takes obtained.Correlative study shows jervine to plant disease such as brown rot, gray mold, soft rot, scab, bacillary perforation
Disease, downy mildew, phytophthora root rot, powdery mildew, late blight, dry rot have good inhibiting effect.
Zhongshengmycin belongs to N- glycoside antibiotic, and antimicrobial spectrum is very wide, can resist Gram-positive, negative bacteria well,
Multiple diseases caused by mycobacteria, saccharomycete and filamentous fungi.To Bacteria erwinia, angular leaf spot fungus, leaf spot bacteria, rice blast
Bacterium, Target spot pathogen, gibberellic hypha etc. all have apparent antibacterial activity.Its mechanism of action is：By inhibiting microorganism albumen
Matter synthesizes and keeps microorganism dead；Made by making der Pilz deformation of filament, inhibiting spore germination or directly killing the modes such as spore
For filamentous fungi；The generation that the precursor substance of protective plant protecting agent and lignin in plant can be stimulated, to improve the disease-resistant of plant
Ability increases income conducive to crop yield.
Graphene oxide (GO) is a kind of two dimensional surface material of monoatomic layer, has prodigious specific surface area.Due to GO
There are many oxygen-containing groups on surface, so GO also has good hydrophily.The sterilization mechanism of GO materials is substantially divided at present
For following 3 kinds of mainstream opinions：The edge of nano material damaging cells film as blade leads to intracellular DNA, RNA and thin
Born of the same parents' device etc. leaks into extracellularly, leads to cell death；The oxidative pressure that material generates can be with the enzyme and albumen of oxidizing bacteria cell
DNA can be oxidizing to and untwist and be broken, while can also aoxidize mitochondria by matter, and energy supply is made to go wrong；Nano flake packet
Cell membrane is wrapped up in and wound, bacterium is come out with ambient environment, some important channels blocked on cell membrane allow cell cannot
Energy exchange is generated with surrounding.
On the one hand, jervine and Zhongshengmycin all have higher bactericidal activity, if but long-term exclusive use, it is easy to make
Plant germ develops immunity to drugs, and then generates the problems such as dosage increases, preventive effect reduces, the lasting period shortens.Meanwhile pesticide is anti-
Multiple use will also result in the degradation rate decline of pesticide in the soil, be unfavorable for very much environment sustainable development.On the other hand, stone is aoxidized
Although black alkene bactericidal effect is fine, long-term be used alone can cause normal plant cells a degree of damage.It will be different
The active ingredient of the mechanism of action is reasonably compounded, and is that the damage that delays disease to develop immunity to drugs and reduce to host plant is non-
Normal effective common method, and can be according to the effect in production application, to judge that this compounding is synergistic effect or short of money
Anti- effect.But for jervine, Zhongshengmycin and the compounded combination of graphene oxide three, there has been no excessively related reports at present
Road.
Invention content
The purpose of the invention is to overcome the above-mentioned deficiency of the prior art, a kind of killing for prevention and control soft rot of Chinese cabbage is provided
Bacteria composition, for the present invention by the way that graphene oxide and jervine, Zhongshengmycin to be used in compounding, three belongs to different role mechanism
Fungicide, mutual mixture not will produce conflict；There is good synergistic function in a certain range, preventive effect is higher than single
Agent, drug effect are more longlasting.
To achieve the goals above, the present invention is achieved by following scheme：
A kind of bactericidal composition of prevention and control soft rot of Chinese cabbage, includes each component of following weight percent：Jervine 5~
20%, Zhongshengmycin 5~10%, graphene oxide 0.1~0.5%, surplus auxiliary agent；The dosage form of the bactericidal composition is wettable
Any one of property pulvis, suspending agent or water dispersible granules.
Three kinds of ingredient mechanism of action points are different in the present composition, and the present inventor passes through a large number of experiments recipe determination, carries
Gone out a kind of Compositional type bactericidal composition of the present invention, i.e., using jervine, Zhongshengmycin and graphene oxide as active ingredient, into
Capable Recompounded multielement bactericidal composition.Since the mechanism of action point of these three ingredients is different, by jervine, Zhongshengmycin and oxidation
Graphene compounds in certain proportion, has good synergy synergistic effect, substantially increases germicidal efficiency, and can prolong well
The generation of slow Disease Resistance, expands fungicidal spectrum, reduces drug cost, improves practical preventive effect, can be used for preventing soft rot of Chinese cabbage.
Preferably, when bactericidal composition is wettable powder, include each component of following weight percent：Jervine 5
~20%, Zhongshengmycin 6~10%, graphene oxide 0.1~0.5%, dispersant 1~10%, wetting agent 1~10%, filler 1
~25%.Jervine, Zhongshengmycin, graphene oxide and dispersant, wetting agent and filler are mixed by above-mentioned weight proportion,
Uniform stirring in a stirring kettle remixes uniformly, obtains wettable powder bactericidal composition after being smashed by air jet mill.
Preferably, when bactericidal composition is suspending agent, include each component of following weight percent：Jervine 5~
10%, Zhongshengmycin 5~7%, graphene oxide 0.1~0.5%, dispersant 5~10%, antifreezing agent 1~5%, thickener 0.1
~1%, antifoaming agent 0.1~0.8%, excess water.The specific production stage of the suspending agent is：First auxiliary agent is mixed by above-mentioned proportioning
It closes, is uniformly mixed through high speed shear, adds jervine, Zhongshengmycin and graphene oxide, abrading-ball 2~3 is small in ball crusher
When, so that a diameter is below 5mm, obtains the bactericidal composition of aqueous suspension formulations.
Preferably, when bactericidal composition is water dispersible granules, include each component of following weight percent：Jervine 5
~15%, Zhongshengmycin 5~10%, graphene oxide 0.1~0.5%, dispersant 5~10%, wetting agent 4~10%, disintegration
Agent 1~5%, filler 1~25%.The specific production stage of the aqueous emulsion is：By jervine, Zhongshengmycin, graphene oxide, divide
Powder, wetting agent, disintegrant and filler are uniformly mixed, and are crushed with micro jet, and through mediating, fluid bed is then added
It is granulated, dried, sieved in granulating and drying machine, obtain the bactericidal composition of water dispersant.
Preferably, the dispersant is aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, fatty acid glyceride gather
At least one of ethylene oxide ether, poloxamer.
Preferably, the wetting agent is in lauryl sodium sulfate, calcium dodecyl benzene sulfonate, alkylnaphthalene sulfonate
It is at least one.
Preferably, the filler is at least one of talcum powder, kaolin, diatomite, bentonite.
Preferably, the antifreezing agent is at least one of ethylene glycol, propylene glycol, glycerine, polyethylene glycol；Described
Thickener be xanthans, hydroxymethyl cellulose, hydroxyethyl cellulose, methylcellulose, aluminum magnesium silicate, in polyvinyl alcohol at least
It is a kind of；The antifoaming agent is at least one of sodium citrate, resorcinol.
Preferably, the disintegrant is at least one of urea, ammonium sulfate, aluminium chloride, citric acid, sodium bicarbonate.
Compared with prior art, the invention has the advantages that：
For the present invention by the way that graphene oxide and jervine, Zhongshengmycin to be used in compounding, three belongs to different role mechanism
Fungicide, mutual mixture not will produce conflict；There is good synergistic function in a certain range, preventive effect is higher than single
Agent, drug effect is more longlasting, substantially increases germicidal efficiency, has good control effect to soft rot of Chinese cabbage；It will not make pathogen
It develops immunity to drugs, expands fungicidal spectrum, reduce dosage and cost, can be used for the prevention of agricultural production crop disease.
Specific implementation mode
The present invention is made with reference to specific embodiment and further being elaborated, the embodiment is served only for explaining this
Invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is normal unless otherwise specified
Rule method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
The incidence that whole strain blade is investigated according to experiment grade scale, calculates disease index and control effect.According to grandson
Yun Peifa (《The Simple Calculation of co-toxicity coefficient and its application》, Huang attain clearly, 1993) calculate medicament toxicity index grade altogether poison be
Number (CTC).CTC≤80, composition show as antagonism；80<CTC<120, composition shows as summation action；CTC≥
120, composition shows as synergistic effect.
1 20% jervine Zhongshengmycin graphene oxide wettable powder of embodiment
By 12% jervine, 7.5% Zhongshengmycin, 0.5% graphene oxide, 10% poloxamer, 9% dodecyl sulphur
Sour sodium and the mixing of 25% kaolin, uniform stirring, remixes uniformly, is made 20% after being smashed by air jet mill in a stirring kettle
Jervine Zhongshengmycin graphene oxide wettable powder.
2 20% jervine Zhongshengmycin graphene oxide wettable powder of embodiment
By 13.7% jervine, 6% Zhongshengmycin, 0.3% graphene oxide, 8% polyoxyethylene carboxylate, 9%0
Dialkyl benzene sulfonic acids calcium, 20% diatomite, 5% bentonite mix, in a stirring kettle uniform stirring, after being smashed by air jet mill
It remixes uniformly, 20% jervine Zhongshengmycin graphene oxide wettable powder is made.
3 15% jervine Zhongshengmycin graphene oxide suspending agent of embodiment
By 7% aliphatic amine polyoxyethylene ether, 3% polyethylene glycol, 0.5% hydroxymethyl cellulose, 0.4% sodium citrate and
74.1% water mixes, and after being uniformly mixed through high speed shear, adds 9% jervine, 5.5% Zhongshengmycin and 0.5% oxidation stone
Black alkene, abrading-ball 2~3 hours in ball crusher, makes a diameter be below 5mm, and 15% jervine Zhongshengmycin oxygen is made
Graphite alkene suspending agent.
4 15% jervine Zhongshengmycin graphene oxide suspending agent of embodiment
10% poloxamer, 4% glycerine, 1.0% xanthans, 0.6% sodium citrate and 69.4% water are mixed, through height
After fast shear-mixed is uniform, 8.7% jervine, 6% Zhongshengmycin and 0.3% graphene oxide are added, is ground in ball crusher
Ball 2~3 hours, makes a diameter be below 5mm, and 15% jervine Zhongshengmycin graphene oxide suspending agent is made.
5 20% jervine Zhongshengmycin graphene oxide water dispersible granules of embodiment
By 14% jervine, 5.5% Zhongshengmycin, 0.5% graphene oxide, 8% poloxamer, 4% dodecyl sulphur
Sour sodium, 5% calcium dodecyl benzene sulfonate, 3% urea, 20% kaolin are uniformly mixed, and are crushed with micro jet, through pinching
Close, be then added in fluidized-bed granulation dryer be granulated, dried, sieve after sampling analysis, in obtained 20% jervine
Raw rhzomorph graphene oxide water dispersible granules.
6 25% jervine Zhongshengmycin graphene oxide water dispersible granules of embodiment
By 15% jervine, 9.7% Zhongshengmycin, 0.3% graphene oxide, 5% glycerol fatty acid ester polyoxyethylene ethe,
6% calcium dodecyl benzene sulfonate, 4.5% ammonium sulfate, 24.5% kaolin are uniformly mixed, and are crushed with micro jet, are passed through
Mediate, be then added in fluidized-bed granulation dryer be granulated, dried, sieve after sampling analysis, obtained 25% jervine
Zhongshengmycin graphene oxide water dispersible granules.
Bacteriostatic activity of the 7 Examples 1 to 6 bactericidal composition of embodiment to Chinese cabbage soft rot bacteria
Bacteriostatic activity of the Examples 1 to 6 bactericidal composition to Chinese cabbage soft rot bacteria is measured using micro-dilution method.Step
It is as follows：
It will be in examination bacterium access Mueller-Hinton broth bouillons, 48h being cultivated in 35 DEG C, until turning after slight haziness
Enter in 0.9% physiological saline, adjusts that (assay method is to make it under 650nm wavelength using spectrophotometer to Maxwell turbidity 0.5
Absorbance be 0.020), then with Mueller-Hinton broth bouillons dilute 200 times it is spare.The bacterium solution diluted is connected to 96
In orifice plate, per 100 μ L of hole liquid volume added, then it is separately added into Examples 1 to 6 bactericidal composition, using streptomycin sulphate as positive control,
Not connect the processing of bacterium as negative control with bacteria culture fluid and dosing (Examples 1 to 6 bactericidal composition).96 holes that will be handled well
Plate is placed in 35 DEG C of incubators and cultivates 20h, and light transmittance is measured under 650nm wavelength, records Examples 1 to 6 bactericidal composition pair
For trying the inhibition growth minimum concentration (MIC) of bacterium, it is the standard for inhibiting growth to be more than 85% with light transmittance, the results are shown in Table 1.
Minimum inhibitory concentration (MIC) measurement result of 1 Examples 1 to 6 bactericidal composition of table to Chinese cabbage soft rot bacteria
| Processing | Minimum inhibitory concentration MIC (μ g/mL) |
| Embodiment 1 | 20 |
| Embodiment 2 | 18 |
| Embodiment 3 | 21 |
| Embodiment 4 | 26 |
| Embodiment 5 | 16 |
| Embodiment 6 | 19 |
| Streptomycin sulphate | 120 |
The result shows that 1~6 bactericidal composition of the embodiment of the present invention is apparently higher than the bacteriostatic activity of Chinese cabbage soft rot bacteria
Streptomycin sulphate.
The toxicity test of 8 jervine of embodiment, Zhongshengmycin and graphene oxide to Erwinia
Jervine, Zhongshengmycin and graphene oxide compounding try Chinese cabbage soft rot bacteria Erwinia toxicity test
It tests, specifically matches and the results are shown in Table 2~3.
2 jervine of table, Zhongshengmycin, graphene oxide single dose are to Erwinia survey toxicity index
| Processing | EC50(μg/mL) | TI |
| Jervine | 0.62 | 100 |
| Zhongshengmycin | 2.19 | 28.31 |
| Graphene oxide | 25.59 | 2.42 |
3 jervine of table, Zhongshengmycin and graphene oxide different proportion compounding are to Erwinia toxicity test result point
Analysis
The result shows that jervine, Zhongshengmycin and graphene oxide significantly improve the control effect of soft rot of Chinese cabbage,
Three's compound proportion has significant synergistic effect, co-toxicity coefficient 150 or more, to increase Erwinia within the scope of the present invention
Effect effect is apparent；And three's compound proportion is not within the scope of the present invention, to cucurbita pepo Bacteria erwinia can only achieve summation action,
Co-toxicity coefficient is less than 120.
9 field efficacy confirmatory experiment of embodiment
Test method：It is once sprayed at the initial stage of a disease, each handles 3 cells, each cell 20m2.Before medicine and
10 days investigation statistics incidences after medicine, 5 grab sample of each cell investigate the lesion area in whole strain per blade and account for blade
The percentage of area calculates disease index and control effect.Every group of pharmaceutical quantities sprayed are with active constituent content in composition
It is all mutually foundation.
Field experiment is carried out to soft rot of Chinese cabbage using Examples 1 to 6 bactericidal composition, the results are shown in Table 4：
4 soft rot of Chinese cabbage disease index of table and control effect
The result shows that jervine, Zhongshengmycin and graphene oxide compounding are notable to the control effect of soft rot of Chinese cabbage.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of shield range can also be made on the basis of above description and thinking for those of ordinary skill in the art
Other various forms of variations or variation, there is no necessity and possibility to exhaust all the enbodiments.It is all the present invention
All any modification, equivalent and improvement etc., should be included in the protection of the claims in the present invention made by within spirit and principle
Within the scope of.
Claims (9)
1. a kind of bactericidal composition of prevention and control soft rot of Chinese cabbage, which is characterized in that include each component of following weight percent：
Jervine 5~20%, Zhongshengmycin 5~10%, graphene oxide 0.1~0.5%, surplus auxiliary agent；The dosage form of the bactericidal composition
For any one of wettable powder, suspending agent or water dispersible granules.
2. bactericidal composition according to claim 1, which is characterized in that when bactericidal composition is wettable powder, packet
Include each component of following weight percent：Jervine 5~20%, Zhongshengmycin 6~10%, graphene oxide 0.1~0.5%, dispersion
Agent 1~10%, wetting agent 1~10%, filler 1~25%.
3. bactericidal composition according to claim 1, which is characterized in that when bactericidal composition is suspending agent, including with
The each component of lower weight percent：Jervine 5~10%, Zhongshengmycin 5~7%, graphene oxide 0.1~0.5%, dispersant 5~
10%, antifreezing agent 1~5%, thickener 0.1~1%, antifoaming agent 0.1~0.8%, excess water.
4. bactericidal composition according to claim 1, which is characterized in that when bactericidal composition is water dispersible granules, packet
Include each component of following weight percent：Jervine 5~15%, Zhongshengmycin 5~10%, graphene oxide 0.1~0.5%, dispersion
Agent 5~10%, wetting agent 4~10%, disintegrant 1~5%, filler 1~25%.
5. according to claim 2~4 any one of them bactericidal composition, which is characterized in that the dispersant is fatty amine
At least one of polyoxyethylene ether, polyoxyethylene carboxylate, glycerol fatty acid ester polyoxyethylene ethe, poloxamer.
6. according to any bactericidal composition of claim 2 or 4, which is characterized in that the wetting agent is dodecyl
At least one of sodium sulphate, calcium dodecyl benzene sulfonate, alkylnaphthalene sulfonate.
7. according to any bactericidal composition of claim 2 or 4, which is characterized in that the filler is talcum powder, kaolinite
At least one of soil, diatomite, bentonite.
8. bactericidal composition according to claim 3, which is characterized in that the antifreezing agent is ethylene glycol, propylene glycol, third
At least one of triol, polyethylene glycol；The thickener is xanthans, hydroxymethyl cellulose, hydroxyethyl cellulose, methyl
At least one of cellulose, aluminum magnesium silicate, polyvinyl alcohol；The antifoaming agent is sodium citrate, in resorcinol at least
It is a kind of.
9. bactericidal composition according to claim 4, which is characterized in that the disintegrant is urea, ammonium sulfate, chlorination
At least one of aluminium, citric acid, sodium bicarbonate.
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|---|---|---|---|
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Application publication date: 20180817 |