CN108554655B - Platelet-rich plasma separation device and separation method - Google Patents
Platelet-rich plasma separation device and separation method Download PDFInfo
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- CN108554655B CN108554655B CN201810004218.2A CN201810004218A CN108554655B CN 108554655 B CN108554655 B CN 108554655B CN 201810004218 A CN201810004218 A CN 201810004218A CN 108554655 B CN108554655 B CN 108554655B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
Abstract
The invention discloses a platelet-rich plasma separation device and a separation method, the platelet-rich plasma separation device comprises a blood collection tube for collecting blood, a three-way joint, an injector, a long needle assembly, a third rubber plug and a fourth rubber plug, wherein the third rubber plug and the fourth rubber plug are provided with air filter elements, the blood collection tube for collecting blood is provided with a cavity with an upward opening and a first rubber plug covering the opening, the three-way joint is provided with a first port, a second port and a thick needle head capable of being inserted through the first rubber plug, the injector comprises a syringe with a third port, the long needle assembly comprises a needle tube, a connecting sleeve and a second rubber plug, the needle tube is provided with a first end and a second end, the second end is sequentially inserted through the connecting sleeve and the second rubber plug, and the first end protrudes out of the connecting sleeve in the direction deviating from the second end. The invention aims to provide a platelet-rich plasma separation device and a separation method which are simple in structure, simple in operation and rapid in extraction.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a platelet-rich plasma separation device and a separation method.
Background
The platelet is a platelet concentrate obtained by centrifuging autologous whole blood, is only one of the visible components in the blood of mammals, is an anucleate vesicle with an irregular shape, and has important functions in the physiological and pathological processes of hemostasis, wound healing, inflammatory reaction, thrombosis, organ transplant rejection and the like. Platelets are rich in large amounts and high concentrations of growth factors.
In the field of clinical medicine or medical science, it is often necessary to extract Platelet-rich plasma (PRP) from blood. PRP can play the role of rapid hemostasis, pain relief, wound healing acceleration or local accelerated growth and the like, and can greatly reduce the formation probability of postoperative scars. With the continuous progress of medical technology, PRP is widely used in various surgical operations, cardiac operations and plastic surgery, wherein the PRP is more prominently used in medical cosmetology, and how to rapidly extract PRP from blood becomes an important prerequisite for the best PRP operation due to the urgency of operation time.
Platelet-rich plasma (PRP) is plasma containing high-concentration platelets obtained by concentrating and separating blood, and has been widely used in clinical repair of bone and soft tissue injuries. The main action mechanism is that after the platelet in PRP is activated, the platelet releases the synergistic action of various high-concentration growth factors to promote the proliferation and differentiation of local repair cells and the synthesis of extracellular matrix, thereby enhancing the tissue regeneration and repair capability. Compared with the traditional method, the traditional Chinese medicine composition has especially obvious treatment effect on certain tissues which are difficult to heal of a human body, such as diabetic foot wound surfaces, bedsores, lower limb venous ulcers, skin necrosis after radiotherapy, bone nonunion, tendon cartilage injuries and the like.
In the prior art, the PRP is prepared by centrifuging for multiple times and repeatedly replacing a centrifugal tube, so that the pollution probability is increased in the operation process, platelets are easily activated in advance, and the activity of the finally obtained PRP is reduced. The PRP extraction device has multiple components, complex integral structure and complex operation, delays the extraction speed of PRP, needs to be transferred in a plurality of containers, and increases the chances of platelet activation and pollution.
Disclosure of Invention
The invention mainly aims to provide a platelet-rich plasma separation device and a separation method, and aims to provide a platelet-rich plasma separation device and a separation method which are simple in structure, simple in operation and rapid in extraction.
In order to achieve the above object, the present invention provides a platelet rich plasma separation device, comprising:
the blood collection tube is used for collecting blood and is provided with a cavity with an upward opening and a first rubber plug covering the opening;
the three-way joint is provided with a first port, a second port and a thick needle head which can be inserted through the first rubber plug, wherein the first port and the second port are communicated with each other;
a syringe comprising a barrel having a third port;
the long needle assembly comprises a needle tube, a connecting sleeve and a second rubber plug, the needle tube is provided with a first end and a second end, the second end is sequentially inserted through the connecting sleeve and the second rubber plug, and the first end protrudes out of the connecting sleeve in the direction departing from the second end;
a third rubber plug and a fourth rubber plug with an air filter element, wherein the third rubber plug is selectively installed in the second port, and the fourth rubber plug is selectively installed in the connecting sleeve;
wherein the platelet rich plasma separation device has a first installed state and a second installed state, the first installed state comprising: the third rubber plug is arranged on the second port, the thick needle head of the three-way joint provided with the third rubber plug is inserted into the first rubber plug, the second end of the needle tube penetrates through the first port and the thick needle head and extends into the containing cavity, the second rubber plug is arranged on the first port, and the connecting sleeve is arranged on the third port of the injection tube;
the second mounting method comprises the following steps: the fourth rubber plug is installed in the connecting sleeve, the thick needle head of the three-way joint is inserted into the first rubber plug, the second end of the needle tube penetrates through the first port and the thick needle head and extends into the containing cavity, the second rubber plug is installed on the first port, and the third port of the injection tube is installed on the second port.
Preferably, one of the third port and the second port has an external thread on an outer side wall thereof, and the other has an internal thread matching the external thread on an inner side wall thereof.
Preferably, the outer side wall of the third port has an external thread, and the inner side wall of the connecting sleeve has an internal thread matched with the external thread.
In order to achieve the above object, the present invention also provides a method for separating platelet-rich plasma using the above platelet-rich plasma separation apparatus, the platelet-rich plasma separation apparatus including:
the blood collection tube is used for collecting blood and is provided with a cavity with an upward opening and a first rubber plug covering the opening;
the three-way joint is provided with a first port, a second port and a thick needle head which can be inserted through the first rubber plug, wherein the first port and the second port are communicated with each other;
a syringe comprising a barrel having a third port;
the long needle assembly comprises a needle tube, a connecting sleeve and a second rubber plug, the needle tube is provided with a first end and a second end, the second end is sequentially inserted through the connecting sleeve and the second rubber plug, and the first end protrudes out of the connecting sleeve in the direction departing from the second end;
a third rubber plug and a fourth rubber plug with an air filter element, wherein the third rubber plug is selectively installed in the second port, and the fourth rubber plug is selectively installed in the connecting sleeve;
the separation method of the platelet rich plasma comprises the following steps:
the blood collection tube filled with collected blood is placed right side by side and subjected to first centrifugation, and is separated into a first part containing red blood cells and a second part containing platelet plasma;
installing the third rubber plug in the second port, inserting the thick needle head through the first rubber plug, inserting the second end of the needle tube through the first port and the thick needle head and extending into the bottom of the cavity, installing the second rubber plug in the first port, and installing the connecting sleeve on the third port to suck a first part into the injection tube;
detaching the syringe filled with the first part from the connecting sleeve, installing the fourth rubber plug on the connecting sleeve, and centrifuging the blood collection tube for the second time until the second part sinks to the bottom of the blood collection tube;
and detaching the fourth rubber plug from the connecting sleeve, installing the connecting sleeve on the third port of the emptied injector, and lifting the needle tube to the upper position of the second part to suck the upper liquid of the second part into the injection tube, wherein the rest part in the blood collection tube is the platelet-rich plasma.
Preferably, the centrifugal force of the first centrifugation is 250 g-350 g, and the centrifugation time is 10 min-20 min.
Preferably, the centrifugal force of the second centrifugation is 800 g-1000 g, and the centrifugation time is 10 min-20 min.
In order to achieve the above object, the present invention also provides a method for separating platelet-rich plasma using the above platelet-rich plasma separation apparatus, the platelet-rich plasma separation apparatus including:
the blood collection tube is used for collecting blood and is provided with a cavity with an upward opening and a first rubber plug covering the opening;
the three-way joint is provided with a first port, a second port and a thick needle head which can be inserted through the first rubber plug, wherein the first port and the second port are communicated with each other;
a syringe comprising a barrel having a third port;
the long needle assembly comprises a needle tube, a connecting sleeve and a second rubber plug, the needle tube is provided with a first end and a second end, the second end is sequentially inserted through the connecting sleeve and the second rubber plug, and the first end protrudes out of the connecting sleeve in the direction departing from the second end;
a third rubber plug and a fourth rubber plug with an air filter element, wherein the third rubber plug is selectively installed in the second port, and the fourth rubber plug is selectively installed in the connecting sleeve;
the separation method of the platelet rich plasma comprises the following steps:
inverting the blood collection tube filled with collected blood and performing first centrifugation to separate the blood collection tube into a first part containing red blood cells and a second part containing platelet plasma;
installing the fourth rubber plug in the connecting sleeve, inserting the thick needle head through the first rubber plug, penetrating the second end of the needle tube through the first port and the thick needle head and extending into the bottom of the cavity, installing the second rubber plug in the first port, and installing the third port on the second port to suck the first part into the injection tube;
detaching the injector filled with the first part from the second port, installing the third rubber plug on the second port, and rightly arranging the blood collection tube and performing second centrifugation to enable the second part to sink to the bottom of the blood collection tube;
and detaching the fourth rubber plug from the connecting sleeve, installing the connecting sleeve on the third port of the emptied injector, and lifting the needle tube to the upper position of the second part to suck the upper liquid of the second part into the injection tube, wherein the rest part in the blood collection tube is the platelet-rich plasma.
Preferably, the centrifugal force of the first centrifugation is 250 g-350 g, and the centrifugation time is 10 min-20 min.
Preferably, the centrifugal force of the second centrifugation is 800 g-1000 g, and the centrifugation time is 10 min-20 min.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a schematic exploded view of an embodiment of a platelet rich plasma separation device according to the present invention;
FIG. 2 is a schematic flow chart of an embodiment of a separation method for separating platelet rich plasma using a platelet rich plasma separation device;
fig. 3 is a schematic flow chart of another embodiment of the separation method for separating platelet-rich plasma using the platelet-rich plasma separation device.
The reference numbers illustrate:
| reference numerals | Name (R) | Reference numerals | Name (R) |
| 100 | Platelet-rich plasma separation device | 4 | Long needle assembly |
| 1 | Blood collection tube | 41 | Needle tube |
| 11 | Containing chamber | 41a | First end |
| 12 | First rubber plug | 41b | Second end |
| 2 | Three-way joint | 42 | Connecting sleeve |
| 21 | First port | 43 | Second rubber plug |
| 22 | Second port | 5 | Third rubber plug |
| 23 | Thick needle head | 6 | Fourth rubber plug |
| 3 | Syringe with a needle | 200 | Blood, blood-enriching agent and method for producing the same |
| 31 | Injection tube | 210 | The first part |
| 311 | Third port | 220 | The second part |
The objects, features and advantages of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship between the components, the movement situation, and the like in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indications are changed accordingly.
In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Platelet rich plasma separation device 100
Fig. 1 shows an exploded view of a platelet rich plasma separation device 100 according to a first embodiment of the present invention. Referring to fig. 1, the present invention provides a platelet-rich plasma separation device 100, the platelet-rich plasma separation device 100 includes a blood collection tube 1, a three-way joint 2, an injector 3, a long needle assembly 4, a third rubber plug 5 and a fourth rubber plug 6, the blood collection tube 1 is used for collecting blood 200, the blood collection tube 1 has a cavity 11 with an upward opening and a first rubber plug 12 covering the opening; the three-way joint 2 is provided with a first port 21, a second port 22 and a thick needle 23 which can be inserted through the first rubber plug 12, wherein the first port 21 and the second port 22 are communicated with each other; the injector 3 comprises a barrel 31 having a third port 311; the long needle assembly 4 comprises a needle tube 41, a connecting sleeve 42 and a second rubber plug 43, the needle tube 41 has a first end 41a and a second end 41b, the second end 41b is sequentially inserted through the connecting sleeve 42 and the second rubber plug 43, and the first end 41a protrudes out of the connecting sleeve 42 in a direction away from the second end 41 b; the third rubber plug 5 and the fourth rubber plug 6 are both provided with air filter elements, the third rubber plug 5 can be selectively installed in the second port 22, and the fourth rubber plug 6 can be selectively installed in the connecting sleeve 42; wherein the platelet rich plasma separation device has a first installed state and a second installed state, the first installed state comprising: the third rubber plug 5 is arranged on the second port 22, the thick needle 23 of the three-way joint 2 provided with the third rubber plug 5 is inserted through the first rubber plug 12, the second end 41b of the needle tube 41 passes through the first port 21 and the thick needle 23 and extends into the cavity 11, the second rubber plug 43 is arranged on the first port 21, and the connecting sleeve 42 is arranged on the third port 311 of the injection tube 31;
the second mounting method comprises the following steps: the fourth rubber plug 6 is installed in the connecting sleeve 42, the thick needle 23 of the three-way joint 2 is inserted through the first rubber plug 12, the second end 41b of the needle tube 41 passes through the first port 21 and the thick needle 23 and extends into the cavity 11, the second rubber plug 43 is installed on the first port 21, and the third port 311 of the injection tube 31 is installed on the second port 22.
The small blood-rich plasma separation device adopted in the prior art has the advantages of multiple component compositions, complex integral structure and complex operation, delays the extraction speed of PRP, needs to be transferred in a plurality of containers, and increases the chances of platelet activation and pollution. The invention can prepare the blood plasma rich in small blood by using simple and easily-obtained tools without replacing containers.
In order to ensure that the syringe 3 does not leak when sucking the liquid, one of the third port 311 and the second port 22 has an external thread on its outer side wall, and the other has an internal thread matching with the external thread. Thus, the self-locking property of the screw connection can ensure that the second port 22 is firmly connected with the third port 311. Since the three-way joint 2 and the syringe 3 are usually made of plastic materials, the connection tightness can be ensured when the second port 22 is connected with the third port 311.
Similarly, the outer side wall of the third port 311 has an external thread, and the inner side wall of the connecting sleeve 42 has an internal thread matched with the external thread. Thus, the self-locking property of the screw connection can ensure that the third port 311 is firmly connected with the connecting sleeve 42. Since the syringe 3 and the connecting sleeve 42 are usually made of plastic materials, the connection tightness of the syringe 3 and the connecting sleeve 42 can be ensured when the connection is carried out.
In order to facilitate visual observation of the blood 200 and the like in the filling syringe 3 and the blood collection tube 1, the syringe 3 and the blood collection tube 1 are preferably transparent or translucent, and the volumes of the syringe 3 and the blood collection tube 1 are not particularly limited. The blood collection tube 1 may be a blood collection tube 1 conventional in the art. The blood collection tube 1 may collect blood 200 directly from a donor with a blood collection needle, and the collected blood 200 is usually whole blood, or platelets obtained from umbilical cord blood or placenta, and is not limited herein.
The material of the first rubber plug 12, the second rubber plug 43, the third rubber plug 5 and the fourth rubber plug 6 may be molded products of polypropylene or rubber (elastomer) in consideration of the reliability of sealing. Thus, the first rubber plug 12, the second rubber plug 43, the third rubber plug 5 and the fourth rubber plug 6 are also conveniently subjected to gamma ray sterilization treatment.
The platelet-rich plasma separation device 100 is installed in at least two ways:
in a first installation manner, please refer to fig. 2 (a), (g), (h) and fig. 3 (f), (g), the third rubber plug 5 is installed on the second port 22, the thick needle 23 of the three-way joint 2 with the third rubber plug 5 installed is inserted through the first rubber plug 12, the second end 41b of the needle tube 41 passes through the first port 21 and the thick needle 23 and extends into the cavity 11, the second rubber plug 43 is installed on the first port 21, and the connection sleeve 42 is installed on the third port 311 of the injection tube 31.
In a second installation manner, please refer to fig. 3 (c), the fourth rubber plug 6 is installed in the connection sleeve 42, the thick needle 23 of the three-way joint 2 is inserted through the first rubber plug 12, the second end 41b of the needle tube 41 passes through the first port 21 and the thick needle 23 and extends into the cavity 11, the second rubber plug 43 is installed on the first port 21, and the third port 311 of the injection tube 31 is installed on the second port 22.
Separation method of platelet-rich plasma-positive method
Fig. 2 shows a schematic flow chart of a separation method of platelet rich plasma according to a second embodiment of the present invention. Referring to fig. 2, the method for separating platelet-rich plasma includes the following steps:
in step S210, the blood collection tube 1 filled with the collected blood 200 is set upright and subjected to a first centrifugation to separate into a first portion 210 containing red blood cells and a second portion 220 containing platelet plasma.
Step S220, the third rubber plug 5 is installed in the second port 22, the thick needle 23 is inserted through the first rubber plug 12, the second end 41b of the needle tube 41 passes through the first port 21 and the thick needle 23 and extends into the bottom of the cavity 11, the second rubber plug 43 is installed in the first port 21, and the connecting sleeve 42 is installed on the third port 311 to suck the first portion 210 into the syringe 31.
Step S230, detaching the syringe 3 filled with the first portion 210 from the connecting sleeve 42, installing the fourth rubber plug 6 on the connecting sleeve 42, and centrifuging the blood collection tube for the second time until the second portion 220 sinks to the bottom of the blood collection tube.
Step S240, detaching the fourth rubber plug 6 from the connection sleeve 42, installing the connection sleeve 42 on the third port 311 of the evacuated injector 3, and lifting the needle tube 41 to the upper position of the second portion 220 to suck the upper liquid of the second portion 220 into the injection tube 31, wherein the remaining portion in the blood collection tube 1 is platelet-rich plasma.
In step S210, referring to fig. 2 (a) and 2 (b), the blood collection tube 1 filled with the collected blood 200 is subjected to a first centrifugation to separate the red blood cell-containing liquid and the platelet-containing plasma. In fig. 2 (b), the upper layer is the second portion 220 containing platelet plasma and the lower layer is the first portion 210 containing red blood cells, mainly because the density of the second portion 220 containing platelet plasma is less than the density of the first portion 210 containing red blood cells.
The blood collection tube 1 filled with the collected blood 200 is subjected to a first centrifugation, wherein the centrifugation force of the first centrifugation is 250 to 350g, and the centrifugation time is 10 to 20 min. Stem cells and the like are easily broken by an excessive centrifugal force due to red blood cells, stem cells and the like contained in the blood 200; too little centrifugal force makes it difficult to separate the blood 200 into a second portion 220 containing platelet plasma and a first portion 210 containing red blood cells. Therefore, the centrifugal force is set to 250g to 350 g. In the prior art, the centrifugal speed is usually set, and the centrifugal force is related to the radius R, wherein R is the distance from the center of the shaft of the centrifuge to the bottom of the test tube of the horizontal centrifuge or the distance from the center of the test tube opening of the vertical centrifuge. If only the centrifugal rotation speed is selected, the centrifugal force changes when the model of the centrifuge changes or the R changes, and the effect is likely to be unstable. The centrifugal force of the first centrifugation of the device is preferably 300g, and the centrifugation time is 15 min.
In step S220, please refer to fig. 2 (c) and fig. 2 (d), the third rubber plug 5 is installed in the second port 22, and since the third rubber plug 5 is provided with the air filter, the three-way joint 2 can be communicated with the outside air through the air filter, so as to prevent the air from directly entering the three-way joint 2 and causing pollution. Insert through thick syringe needle 23 first plug 12, so, be convenient for needle tubing 41 through first opening, thick syringe needle 23 stretch into the appearance intracavity 11 of heparin tube 1, the air after air filter also can get into the appearance intracavity 11 of heparin tube 1 via second port 22, thick syringe needle 23 simultaneously, guarantee the smooth and easy nature when absorbing the liquid in the heparin tube 1.
The second end 41b of the needle tube 41 passes through the first port 21 and the thick needle 23 and extends into the bottom of the cavity 11, so that the bottom of the second end 41b of the needle tube 41 reaches the bottom of the first portion 210 containing red blood cells. A second plug 43 is mounted at the first port 21 to hermetically seal the first port 21 by the second plug 43. The connection sleeve 42 is installed on the third port 311 by hermetically sealing the connection sleeve 42 and the syringe 3. Since the first end 41a of the needle tube 41 protrudes from the connecting sleeve 42 in a direction away from the second end 41b, the first end 41a of the needle tube 41 is communicated with the barrel 31 of the injector 3 when the connecting sleeve 42 is installed on the third port 311. In this state, when the plunger of the syringe 3 is pulled upward, the first portion 210 containing red blood cells in the blood collection tube 1 is drawn into the syringe barrel 31.
In step S230, referring to fig. 2 (e) and fig. 2 (f), the syringe 3 filling the first portion 210 is detached from the connection sleeve 42, the syringe 3 sucking the first portion 210 containing red blood cells is detached, and the first portion 210 containing red blood cells is moved to another container or discarded, etc., without limitation. If it is desired to obtain a purer second portion 220 of the platelet-containing plasma, the first portion 210 of the syringe 3 containing the red blood cells is cleaned and then the syringe 3 is cleaned, sterilized, or the like, or the syringe 3 is directly replaced with a new one for use in a subsequent step. After the syringe 3 is detached from the connecting sleeve 42, the fourth rubber plug 6 is installed on the connecting sleeve 42, and the connecting sleeve 42 and the fourth rubber plug 6 are sealed in an airtight manner, so that air is prevented from directly entering the blood collection tube 1 to cause pollution. Centrifuging the blood collection tube a second time until the second portion 220 sinks to the bottom of the blood collection tube, such that the second portion 220 containing platelets sinks to the bottom of the blood collection tube for subsequent operations. Preferably, the centrifugal force of the second centrifugation is 800 g-1000 g, and the centrifugation time is 10 min-20 min.
The Platelet Rich Plasma (PRP) is plasma containing high concentration of platelets obtained by concentrating and separating the blood 200, and in the second portion 220 after the second centrifugation, since the platelets in the upper layer of the liquid are less and the density of the platelets in the lower layer of the liquid is higher, the upper layer of the liquid needs to be sucked out, and the Platelet Rich Plasma (PRP) is obtained only in the lower layer.
In step S240, please refer to fig. 2 (g), fig. 2 (H) and fig. 2 (j), the fourth rubber plug 6 is detached from the connecting sleeve 42, the connecting sleeve 42 is mounted on the third port 311 of the evacuated injector 3, the needle tube 41 is lifted up to the upper position of the second portion 220 by hermetically sealing the connecting sleeve 42 and the third port 311 (the height difference between the needle tube 41 and the bottom of the blood collection tube 1 in fig. 2 (g) is H, the H value is influenced by the size of the blood collection tube 1, the platelet content in the blood 200, and other factors, so it is not limited herein), so that the upper liquid of the second portion 220 is sucked into the injection tube 31, and the remaining portion in the blood collection tube 1 is platelet rich plasma.
Example 1
S1, a blood collection tube filled with 10ml to 10ml of whole blood taken from a healthy person is placed upright and subjected to a first centrifugation at a centrifugal force of 290g for 11min to separate into a first part 210 containing red blood cells and a second part 220 containing platelet plasma.
S2, installing the third rubber plug 5 in the second port 22, inserting the thick needle 23 through the first rubber plug 12, inserting the second end 41b of the needle tube 41 through the first port 21 and the thick needle 23 and extending into the bottom of the cavity 11, installing the second rubber plug 43 in the first port 21, installing the connecting sleeve 42 on the third port 311, so as to suck the first part 210 into the syringe 31;
s3, the syringe 3 filled with the first part 210 is detached from the connecting sleeve 42, the fourth rubber plug 6 is installed on the connecting sleeve 42, the blood collection tube is centrifuged for the second time until the second part 220 sinks to the bottom of the blood collection tube, the detached syringe 3 is processed to empty the liquid therein, or a new syringe 3 is replaced for standby, the centrifugal force of the second centrifugation is 810g, and the centrifugation time is 19 min.
S4, detaching the fourth rubber plug 6 from the connection sleeve 42, installing the connection sleeve 42 on the third port 311 of the emptied syringe 3, lifting the needle tube 41 upward by 2.5cm to suck the upper liquid of the second portion 220 into the injection tube 31, retaining about 1ml of the second portion 220 at the bottom, and obtaining the remaining portion in the blood collection tube 1 as the platelet-rich plasma.
Example 2
S1, a blood collection tube filled with 10ml to 10ml of whole blood taken from a healthy person is placed upright and subjected to a first centrifugation at a centrifugal force of 310g for 12min to separate into a first part 210 containing red blood cells and a second part 220 containing platelet plasma.
S2, installing the third rubber plug 5 in the second port 22, inserting the thick needle 23 through the first rubber plug 12, inserting the second end 41b of the needle tube 41 through the first port 21 and the thick needle 23 and extending into the bottom of the cavity 11, installing the second rubber plug 43 in the first port 21, installing the connecting sleeve 42 on the third port 311, so as to suck the first part 210 into the syringe 31;
s3, the syringe 3 filled with the first part 210 is detached from the connecting sleeve 42, the fourth rubber plug 6 is installed on the connecting sleeve 42, the blood collection tube is centrifuged for the second time until the second part 220 sinks to the bottom of the blood collection tube, the detached syringe 3 is processed to empty the liquid therein, or a new syringe 3 is replaced for standby, the centrifugal force of the second centrifugation is 870g, and the centrifugation time is 18 min.
S4, detaching the fourth rubber plug 6 from the connection sleeve 42, installing the connection sleeve 42 on the third port 311 of the emptied syringe 3, lifting the needle tube 41 upwards by 2.7cm to suck the upper liquid of the second portion 220 into the injection tube 31, retaining about 1.1ml of the second portion 220 at the bottom, and obtaining the remaining portion of the blood collection tube 1 as platelet-rich plasma.
Inversion method for separating platelet-rich plasma
Fig. 3 is a schematic flow chart showing a method for separating platelet-rich plasma according to a third embodiment of the present invention. Referring to fig. 3, the method for separating platelet-rich plasma includes the following steps:
a step S310 of inverting the blood collection tube 1 filled with the collected blood 200 and performing a first centrifugation to separate the blood collection tube into a first portion 210 containing red blood cells and a second portion 220 containing platelet plasma;
step S320, installing the fourth rubber plug 6 in the connecting sleeve 42, inserting the thick needle 23 through the first rubber plug 12, inserting the second end 41b of the needle tube 41 through the first port 21 and the thick needle 23 and extending into the bottom of the cavity 11, installing the second rubber plug 43 at the first port 21, and installing the third port 311 on the second port 22 to suck the first part 210 into the syringe 31;
step S330 of detaching the syringe 3 filled with the first portion 210 from the second port 22, attaching the third rubber stopper 5 to the second port 22, setting the blood collection tube upright, and performing a second centrifugation to sink the second portion 220 to the bottom of the blood collection tube;
step S340, detaching the fourth rubber plug 6 from the connection sleeve 42, installing the connection sleeve 42 on the third port 311 of the evacuated injector 3, and lifting the needle tube 41 to the upper position of the second portion 220 to suck the upper liquid of the second portion 220 into the injection tube 31, wherein the remaining portion in the blood collection tube 1 is platelet-rich plasma.
In step S310, referring to fig. 3 (a) and 3 (b), the blood collection tube 1 filled with the collected blood 200 is inverted and then subjected to a first centrifugation to separate the red blood cell-containing liquid and the platelet-containing plasma. In fig. 3 (b), the upper layer is the second portion 220 containing platelet plasma and the lower layer is the first portion 210 containing red blood cells, mainly because the density of the second portion 220 containing platelet plasma is less than the density of the first portion 210 containing red blood cells.
The blood collection tube 1 filled with the collected blood 200 is subjected to a first centrifugation, wherein the centrifugation force of the first centrifugation is 250 to 350g, and the centrifugation time is 10 to 20 min. Stem cells and the like are easily broken by an excessive centrifugal force due to red blood cells, stem cells and the like contained in the blood 200; too little centrifugal force makes it difficult to separate the blood 200 into a second portion 220 containing platelet plasma and a first portion 210 containing red blood cells. Therefore, the centrifugal force is set to 250g to 350 g. In the prior art, the centrifugal speed is usually set, and the centrifugal force is related to the radius R, wherein R is the distance from the center of the shaft of the centrifuge to the bottom of the test tube of the horizontal centrifuge or the distance from the center of the test tube opening of the vertical centrifuge. If only the centrifugal rotation speed is selected, the centrifugal force changes when the model of the centrifuge changes or the R changes, and the effect is likely to be unstable. The centrifugal force of the first centrifugation of the device is preferably 300g, and the centrifugation time is 15 min.
In step S320, please refer to fig. 3 (c), the fourth rubber plug 6 is installed in the connection sleeve 42, the connection sleeve 42 and the fourth rubber plug 6 are hermetically sealed, and the thick needle 23 is inserted through the first rubber plug 12, because the liquid near the opening of the inverted blood collection tube 1 is the first portion 210 containing red blood cells, the thick needle 23 can contact the first portion 210 containing red blood cells after being inserted into the first rubber plug 12.
Meanwhile, the thick needle 23 is inserted through the first rubber plug 12, so that the second end 41b of the needle tube 41 penetrates through the first port 21 and the thick needle 23 and extends into the bottom of the cavity 11, the second rubber plug 43 is installed at the first port 21, the first port 21 and the second rubber plug 43 are sealed through air tightness, and at the moment, the atmosphere filtered by the filter element enters the blood collection tube 1 through the needle tube 41. The third port 311 is mounted on the second port 22 to mount the syringe 3 on the second port 22, so that the syringe 3 can suck the liquid into the syringe barrel 31 through the thick needle 23 and the second port 22 to suck the first part 210 into the syringe barrel 31. Because the fourth rubber plug 6 is provided with the air filter element, the air filtered by the air filter element can enter the bottom of the cavity 11 of the blood collection tube 1 through the connecting sleeve 42, the first port 21 and the thick needle 23.
In step S330, please refer to fig. 3 (d) and fig. 3 (e), the syringe 3 filled with the first portion 210 is detached from the second port 22, the third rubber plug 5 is mounted on the second port 22, and the syringe 3 sucked into the first portion 210 containing the red blood cells is detached, so as to move the first portion 210 containing the red blood cells to another container, or discard the same, without limitation. If it is desired to obtain a purer second portion 220 of the platelet-containing plasma, the first portion 210 of the syringe 3 containing the red blood cells is cleaned and then the syringe 3 is cleaned, sterilized, or the like, or the syringe 3 is directly replaced with a new one for use in a subsequent step. The tube is then positioned and centrifuged a second time to allow the second portion 220 to sink to the bottom of the tube.
The second platelet-containing portion 220 is deposited at the bottom of the blood collection tube for subsequent handling. Preferably, the centrifugal force of the second centrifugation is 800 g-1000 g, and the centrifugation time is 10 min-20 min.
The Platelet Rich Plasma (PRP) is plasma containing high concentration of platelets obtained by concentrating and separating the blood 200, and in the second portion 220 after the second centrifugation, since the platelets in the upper layer of the liquid are less and the density of the platelets in the lower layer of the liquid is higher, the upper layer of the liquid needs to be sucked out, and the Platelet Rich Plasma (PRP) is obtained only in the lower layer.
In step S340, please refer to fig. 3 (f), fig. 2 (g) and fig. 2 (H), the fourth rubber plug 6 is detached from the connection sleeve 42, the connection sleeve 42 is mounted on the third port 311 of the evacuated injector 3, the needle tube 41 is lifted up to the upper position of the second portion 220 by hermetically sealing the connection sleeve 42 and the third port 311 (the height difference between the connection sleeve 42 and the bottom of the blood collection tube 1 in fig. 2 (g) is H, the H value is influenced by the size of the blood collection tube 1, the platelet content in the blood 200, and other factors, so that the limitation is not made herein), so as to suck the upper liquid of the second portion 220 into the injection tube 31, and the remaining portion in the blood collection tube 1 is platelet rich plasma.
Example 3
S1, a blood collection tube filled with 10ml of whole blood taken from a healthy person to 10ml is inverted and subjected to a first centrifugation at a centrifugal force of 290g for 11min to separate into a first part 210 containing red blood cells and a second part 220 containing platelet plasma.
S2, installing the fourth rubber plug 6 in the connecting sleeve 42, inserting the thick needle 23 through the first rubber plug 12, inserting the second end 41b of the needle tube 41 through the first port 21 and the thick needle 23 and into the bottom of the cavity 11, installing the second rubber plug 43 at the first port 21, installing the third rubber plug 311 on the second port 22, so as to suck the first part 210 into the injection tube 31;
s3, the syringe 3 filled with the first part 210 is detached from the second port 22, the third rubber plug 5 is installed on the second port 22, the blood collection tube is arranged upright and centrifuged for the second time, the second part 220 is settled at the bottom of the blood collection tube, the detached syringe 3 is processed to empty the liquid therein, or a new syringe 3 is replaced for standby, the centrifugal force of the second centrifugation is 900g, and the centrifugation time is 12 min.
S4, detaching the fourth rubber plug 6 from the connection sleeve 42, installing the connection sleeve 42 on the third port 311 of the emptied syringe 3, lifting the needle tube 41 upward by 2.5cm to suck the upper liquid of the second portion 220 into the injection tube 31, retaining about 1ml of the second portion 220 at the bottom, and obtaining the remaining portion in the blood collection tube 1 as the platelet-rich plasma.
Example 4
S1, a blood collection tube filled with 10ml of whole blood taken from a healthy person to 10ml is inverted and subjected to a first centrifugation at a centrifugal force of 320g for 13min to separate into a first part 210 containing red blood cells and a second part 220 containing platelet plasma.
S2, installing the fourth rubber plug 6 in the connecting sleeve 42, inserting the thick needle 23 through the first rubber plug 12, inserting the second end 41b of the needle tube 41 through the first port 21 and the thick needle 23 and into the bottom of the cavity 11, installing the second rubber plug 43 at the first port 21, installing the third rubber plug 311 on the second port 22, so as to suck the first part 210 into the injection tube 31;
s3, the syringe 3 filled with the first part 210 is detached from the second port 22, the third rubber plug 5 is installed on the second port 22, the blood collection tube is arranged upright and centrifuged for the second time, the second part 220 is settled at the bottom of the blood collection tube, the detached syringe 3 is processed to empty the liquid therein, or a new syringe 3 is replaced for standby, the centrifugal force of the second centrifugation is 850g, and the centrifugation time is 15 min.
S4, detaching the fourth rubber plug 6 from the connection sleeve 42, installing the connection sleeve 42 on the third port 311 of the emptied syringe 3, lifting the needle tube 41 upward by 2.5cm to suck the upper liquid of the second portion 220 into the injection tube 31, retaining about 1ml of the second portion 220 at the bottom, and obtaining the remaining portion in the blood collection tube 1 as the platelet-rich plasma.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (9)
1. A platelet rich plasma separation device comprising:
the blood collection tube is used for collecting blood and is provided with a cavity with an upward opening and a first rubber plug covering the opening;
the three-way joint is provided with a first port, a second port and a thick needle head which can be inserted through the first rubber plug, wherein the first port and the second port are communicated with each other;
a syringe comprising a barrel having a third port;
the long needle assembly comprises a needle tube, a connecting sleeve and a second rubber plug, the needle tube is provided with a first end and a second end, the second end is sequentially inserted through the connecting sleeve and the second rubber plug, and the first end protrudes out of the connecting sleeve in the direction departing from the second end; and the number of the first and second groups,
a third rubber plug and a fourth rubber plug with an air filter element, wherein the third rubber plug is selectively installed in the second port, and the fourth rubber plug is selectively installed in the connecting sleeve;
wherein the platelet rich plasma separation device has a first installed state and a second installed state, the first installed state comprising: the third rubber plug is arranged on the second port, the thick needle head of the three-way joint provided with the third rubber plug is inserted into the first rubber plug, the second end of the needle tube penetrates through the first port and the thick needle head and extends into the containing cavity, the second rubber plug is arranged on the first port, and the connecting sleeve is arranged on the third port of the injection tube;
the second mounting method comprises the following steps: the fourth rubber plug is installed in the connecting sleeve, the thick needle head of the three-way joint is inserted into the first rubber plug, the second end of the needle tube penetrates through the first port and the thick needle head and extends into the containing cavity, the second rubber plug is installed on the first port, and the third port of the injection tube is installed on the second port.
2. The platelet-rich plasma separation device of claim 1 wherein the third port and the second port have external threads on an exterior sidewall and an internal thread on an interior sidewall that mates with the external threads.
3. The platelet-rich plasma separation device of claim 1 wherein the outer sidewall of the third port has external threads and the inner sidewall of the connection sleeve has internal threads matching the external threads.
4. A separation method of platelet-rich plasma using the platelet-rich plasma separation device according to any one of claims 1 to 3, comprising the steps of:
the blood collection tube filled with collected blood is placed right side by side and subjected to first centrifugation, and is separated into a first part containing red blood cells and a second part containing platelet plasma;
installing the third rubber plug in the second port, inserting the thick needle head through the first rubber plug, inserting the second end of the needle tube through the first port and the thick needle head and extending into the bottom of the cavity, installing the second rubber plug in the first port, and installing the connecting sleeve on the third port to suck a first part into the injection tube;
detaching the syringe filled with the first part from the connecting sleeve, installing the fourth rubber plug on the connecting sleeve, and centrifuging the blood collection tube for the second time until the second part sinks to the bottom of the blood collection tube;
and detaching the fourth rubber plug from the connecting sleeve, installing the connecting sleeve on the third port of the emptied injector, and lifting the needle tube to the upper position of the second part to suck the upper liquid of the second part into the injection tube, wherein the rest part in the blood collection tube is the platelet-rich plasma.
5. The method for separating platelet-rich plasma according to claim 4, wherein the first centrifugation is performed at a centrifugal force of 250 to 350g for 10 to 20 min.
6. The method for separating platelet-rich plasma according to claim 4, wherein the second centrifugation is performed at a centrifugal force of 800g to 1000g for 10min to 20 min.
7. A separation method of platelet-rich plasma using the platelet-rich plasma separation device according to any one of claims 1 to 3, comprising the steps of:
inverting the blood collection tube filled with collected blood and performing a first centrifugation to separate into a first portion containing red blood cells and a second portion containing platelet plasma;
installing the fourth rubber plug in the connecting sleeve, inserting the thick needle head through the first rubber plug, penetrating the second end of the needle tube through the first port and the thick needle head and extending into the bottom of the cavity, installing the second rubber plug in the first port, and installing the third port on the second port to suck the first part into the injection tube;
detaching the injector filled with the first part from the second port, installing the third rubber plug on the second port, and rightly arranging the blood collection tube and performing second centrifugation to enable the second part to sink to the bottom of the blood collection tube;
and detaching the fourth rubber plug from the connecting sleeve, installing the connecting sleeve on the third port of the emptied injector, and lifting the needle tube to the upper position of the second part to suck the upper liquid of the second part into the injection tube, wherein the rest part in the blood collection tube is the platelet-rich plasma.
8. The method for separating platelet-rich plasma according to claim 7, wherein the first centrifugation is performed at a centrifugal force of 250 to 350g for 10 to 20 min.
9. The method for separating platelet-rich plasma according to claim 7, wherein the second centrifugation is performed at a centrifugal force of 800g to 1000g for 10min to 20 min.
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| CN105107233A (en) * | 2015-07-24 | 2015-12-02 | 上海市第六人民医院 | Preparation method and device for leukocyte-depleted platelet rich plasma |
| CN106139290A (en) * | 2016-08-01 | 2016-11-23 | 娄域峰 | Integration autologous platelet rich plasma preparation suit |
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