CN108554655A - Platelet rich plasma separator and separation method - Google Patents
Platelet rich plasma separator and separation method Download PDFInfo
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- CN108554655A CN108554655A CN201810004218.2A CN201810004218A CN108554655A CN 108554655 A CN108554655 A CN 108554655A CN 201810004218 A CN201810004218 A CN 201810004218A CN 108554655 A CN108554655 A CN 108554655A
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- port
- rubber plug
- connector sleeve
- centrifugation
- needle
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
Abstract
The invention discloses a kind of platelet rich plasma separator and separation methods, the platelet rich plasma separator includes the heparin tube for acquiring blood, three-way connection, syringe, long needle assemblies and the third rubber plug with air filtering core and the 4th rubber plug, there is heparin tube for acquiring blood opening up vessel and lid to close the first rubber plug of the opening, three-way connection has the first port for the setting that is interconnected, second port and the thick needle that first rubber plug can be inserted through, syringe includes the injection tube for having third port, long needle assemblies, including needle tubing, connector sleeve and the second rubber plug, the needle tubing has a first end and a second end, the second end is inserted through the connector sleeve and the setting of the second rubber plug successively, the first end stretches out the connector sleeve towards the direction away from the second end.The present invention is intended to provide a kind of simple in structure, the easy to operate and quick platelet rich plasma separator of extraction and separation method.
Description
Technical field
The present invention relates to the field of medical instrument technology, more particularly to a kind of platelet rich plasma separator and separation side
Method.
Background technology
Blood platelet is the platelet concentrate that self whole blood obtains after centrifugation, exists only in having in mammalian
One divided is formed, is seedless vesica in irregular shape, in hemostasis, wound healing, inflammatory reaction, thrombosis and organ transplant
It plays an important role in the physiology such as repulsion and pathologic process.Blood platelet is rich in the growth factor of a large amount of high concentrations.
In clinical treatment or doctor U.S. field, the platelet rich plasma (Platelet- in extraction blood is generally required
Rich plasma, PRP).PRP can play the effects that rapid hemostasis, analgesic, accelerating wound healing or local tachyauxesis, can
High degree reduces the formation probability of scar after the operation.With being constantly progressive for medical technology, PRP is widely used in various surgeries
Operation, openheart surgery and medicine plastic operation, wherein upper application is more prominent in terms of medical cosmetology, and because of operating time
It is urgent so that how from blood rapid extraction PRP becomes the important prerequisites of best PRP operations.
Platelet rich plasma (PRP) is the blood plasma containing high concentration blood platelet obtained after detaching pachyemia, by
Extensively using clinically reparation bone and soft tissue injury.Main mechanism be released after platelet activation in PRP it is a variety of
The growth factor of high concentration acts synergistically, and promotes the synthesis of the Proliferation, Differentiation and extracellular matrix of local route repair cell, to
Enhance regeneration and repair ability.The tissue damage of some refractory conjunctions for human body, as the diabetes surface of a wound, bedsore, under
Cutaneous necrosis, bone nonunion, tendon cartilage damage etc. after limb venous ulcer, radiotherapy, therapeutic effect seems compared with conventional method
It is especially apparent.
And the production method of PRP is repeatedly centrifugation in the prior art, changes centrifuge tube repeatedly, it is several that operating process increases pollution
Rate, while easilying lead to blood platelet and activating in advance, the PRP activity finally obtained reduces.The component of PRP extraction elements forms
More, complicated integral structure is cumbersome, has delayed the extraction rate of PRP, and needs to be shifted in multiple containers, increases
Platelet activation and contaminated chance.
Invention content
The main object of the present invention is a kind of platelet rich plasma separator of proposition and separation method, it is desirable to provide one
Kind is simple in structure, easy to operate and extracts quick platelet rich plasma separator and separation method.
To achieve the above object, a kind of platelet rich plasma separator proposed by the present invention, including：
There is heparin tube for acquiring blood opening up vessel and lid to close the first rubber plug of the opening；
Three-way connection has and is interconnected the first port being arranged, second port and can be inserted through first rubber plug
Thick needle；
Syringe includes the injection tube with third port；
Long needle assemblies, including needle tubing, connector sleeve and the second rubber plug, the needle tubing has a first end and a second end, described
Second end is inserted through the connector sleeve and the setting of the second rubber plug successively, and the first end is stretched out towards the direction away from the second end
The connector sleeve；
Third rubber plug with air filtering core and the 4th rubber plug, the third rubber plug are selectively attached to the second port
Interior, the 4th rubber plug is selectively attached in the connector sleeve；
Wherein, the platelet rich plasma separator have the first installation condition and the second installation condition, described first
Installation condition includes：The third rubber plug is mounted in the second port, will be equipped with the threeway of the third rubber plug
The thick needle of connector is inserted through the first rubber plug, and the second end of needle tubing passes through first port and thick needle and stretches into vessel, the second glue
Plug is mounted in first port, and connector sleeve is mounted in the third port of injection tube；
Second of mounting means include：4th rubber plug is mounted in connector sleeve, and the thick needle of three-way connection is inserted through first
The second end of rubber plug, needle tubing passes through first port and thick needle and stretches into vessel, and the second rubber plug is mounted in first port, note
The third port installation of shooting cylinder is on the second port.
Preferably, the third port and the second port have external screw thread on the lateral wall of one of them, another
Madial wall has the internal thread coordinated with the external screw thread.
Preferably, the lateral wall of the third port has external screw thread, have on the madial wall of the connector sleeve with it is described
The internal thread of external screw thread cooperation.
In addition, to achieve the above object, the present invention also provides a kind of separation method of platelet rich plasma, the separation sides
Method uses above-mentioned platelet rich plasma separator, and the platelet rich plasma separator includes：
There is heparin tube for acquiring blood opening up vessel and lid to close the first rubber plug of the opening；
Three-way connection has and is interconnected the first port being arranged, second port and can be inserted through first rubber plug
Thick needle；
Syringe includes the injection tube with third port；
Long needle assemblies, including needle tubing, connector sleeve and the second rubber plug, the needle tubing has a first end and a second end, described
Second end is inserted through the connector sleeve and the setting of the second rubber plug successively, and the first end is stretched out towards the direction away from the second end
The connector sleeve；
Third rubber plug with air filtering core and the 4th rubber plug, the third rubber plug are selectively attached to the second port
Interior, the 4th rubber plug is selectively attached in the connector sleeve；
The separation method of the platelet rich plasma includes the following steps：
Filling acquisition there is the heparin tube of blood just setting and carries out first time centrifugation, the containing red blood cell is separated into
A part and the second part containing thrombocyte plasma；
The third rubber plug is mounted in the second port, the thick needle is inserted through first rubber plug, the needle
The second end of pipe passes through the first port and the thick needle and the bottom for stretching into the vessel, second rubber plug are mounted on
The first port, the connector sleeve are mounted in the third port, and first part is sucked the injection tube；
The syringe for filling the first part is separated into the connector sleeve, and on the connector sleeve described in installation
Second of centrifugation of the heparin tube is sunken to the bottom of the heparin tube by the 4th rubber plug to the second part；
4th rubber plug is separated into the connector sleeve, the connector sleeve is installed to the institute of the syringe to emptying
It states in third port, the needle tubing is up-shifted to the topside position of the second part, by the upper of the second part
Layer liquid sucks the injection tube, and remaining part is rich small blood plate blood plasma in the heparin tube.
Preferably, the centrifugal force of the first time centrifugation is 250g~350g, and centrifugation time is 10min~20min.
Preferably, the centrifugal force of second of centrifugation is 800g~1000g, and centrifugation time is 10min~20min.
In addition, to achieve the above object, the present invention also provides a kind of separation method of platelet rich plasma, the separation sides
Method uses above-mentioned platelet rich plasma separator, and the platelet rich plasma separator includes：
There is heparin tube for acquiring blood opening up vessel and lid to close the first rubber plug of the opening；
Three-way connection has and is interconnected the first port being arranged, second port and can be inserted through first rubber plug
Thick needle；
Syringe includes the injection tube with third port；
Long needle assemblies, including needle tubing, connector sleeve and the second rubber plug, the needle tubing has a first end and a second end, described
Second end is inserted through the connector sleeve and the setting of the second rubber plug successively, and the first end is stretched out towards the direction away from the second end
The connector sleeve；
Third rubber plug with air filtering core and the 4th rubber plug, the third rubber plug are selectively attached to the second port
Interior, the 4th rubber plug is selectively attached in the connector sleeve；
The separation method of the platelet rich plasma includes the following steps：
Filling acquisition there is the heparin tube of blood to be inverted and carries out first time centrifugation, the containing red blood cell is separated into
A part and the second part containing thrombocyte plasma；
4th rubber plug is mounted in the connector sleeve, the thick needle is inserted through first rubber plug, the needle tubing
Second end pass through the first port and the thick needle and the bottom for stretching into the vessel, second rubber plug to be mounted on institute
First port is stated, the third port is mounted in the second port, and first part is sucked the injection tube；
The syringe for filling the first part is separated into the second port, and is installed in the second port
The heparin tube is just installed and is carried out second of centrifugation, the second part is made to be sunken to the blood sampling by the third rubber plug
The bottom of pipe；
4th rubber plug is separated into the connector sleeve, the connector sleeve is installed to the institute of the syringe to emptying
It states in third port, the needle tubing is up-shifted to the topside position of the second part, by the upper of the second part
Layer liquid sucks the injection tube, and remaining part is rich small blood plate blood plasma in the heparin tube.
Preferably, the centrifugal force of the first time centrifugation is 250g~350g, and centrifugation time is 10min~20min.
Preferably, the centrifugal force of second of centrifugation is 800g~1000g, and centrifugation time is 10min~20min.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
The structure shown according to these attached drawings obtains other attached drawings.
Fig. 1 is a kind of decomposition texture schematic diagram of an embodiment of platelet rich plasma separator provided by the invention；
Fig. 2 is the stream of one embodiment of separation method when detaching platelet rich plasma using platelet rich plasma separator
Journey schematic diagram；
Fig. 3 is the another embodiment of separation method when detaching platelet rich plasma using platelet rich plasma separator
Flow diagram.
Drawing reference numeral explanation：
| Label | Title | Label | Title |
| 100 | Platelet rich plasma separator | 4 | Long needle assemblies |
| 1 | Heparin tube | 41 | Needle tubing |
| 11 | Vessel | 41a | First end |
| 12 | First rubber plug | 41b | Second end |
| 2 | Three-way connection | 42 | Connector sleeve |
| 21 | First port | 43 | Second rubber plug |
| 22 | Second port | 5 | Third rubber plug |
| 23 | Thick needle | 6 | 4th rubber plug |
| 3 | Syringe | 200 | Blood |
| 31 | Injection tube | 210 | First part |
| 311 | Third port | 220 | Second part |
The object of the invention is realized, the embodiments will be further described with reference to the accompanying drawings for functional characteristics and advantage.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiment is only a part of the embodiment of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art obtained without creative efforts it is all its
His embodiment, shall fall within the protection scope of the present invention.
If it is to be appreciated that related in the embodiment of the present invention directionality instruction (such as up, down, left, right, before and after ...),
Then directionality instruction be only used for explaining relative position relation under a certain particular pose (as shown in the picture) between each component,
Motion conditions etc., if the particular pose changes, directionality instruction also correspondingly changes correspondingly.
If in addition, relating to the description of " first ", " second " etc. in the embodiment of the present invention, it is somebody's turn to do " first ", " second " etc.
Description be used for description purposes only, be not understood to indicate or imply its relative importance or implicitly indicate indicated skill
The quantity of art feature." first " is defined as a result, the feature of " second " can explicitly or implicitly include at least one spy
Sign.In addition, the technical solution between each embodiment can be combined with each other, but must be with those of ordinary skill in the art's energy
It is enough realize based on, when the knot that conflicting or cannot achieve when will be understood that this technical solution occurs in the combination of technical solution
Conjunction is not present, also not the present invention claims protection domain within.
Platelet rich plasma separator 100
Fig. 1 shows the decomposition diagram of the platelet rich plasma separator 100 of first embodiment of the invention.It please refers to
Fig. 1, the present invention provide a kind of platelet rich plasma separator 100, which includes heparin tube
1, three-way connection 2, syringe 3, long needle assemblies 4 and third rubber plug 5 and the 4th rubber plug 6, the heparin tube 1 is for acquiring blood
200, there is heparin tube 1 opening up vessel 11 and lid to close the first rubber plug 12 of the opening；Three-way connection 2 has mutual
It is connected to first port 21, second port 22 and the thick needle 23 that first rubber plug 12 can be inserted through of setting；Syringe 3 wraps
Include the injection tube 31 with third port 311；Long needle assemblies 4 include needle tubing 41, connector sleeve 42 and the second rubber plug 43, the needle
Pipe 41 has first end 41a and second end 41b, and the second end 41b is inserted through the connector sleeve 42 successively and the second rubber plug 43 is set
It sets, the first end 41a stretches out the connector sleeve 42 towards the direction away from the second end 41b；Third rubber plug 5 and the 4th glue
Plug 6 band air filtering core, the third rubber plug 5 are selectively attached in the second port 22, and the 4th rubber plug 6 is optional
It is mounted in the connector sleeve 42 with selecting；Wherein, the platelet rich plasma separator has the first installation condition and second
Installation condition, first installation condition include：Third rubber plug 5 is mounted in second port 22, will be equipped with third rubber plug 5
The thick needle 23 of three-way connection 2 is inserted through the first rubber plug 12, and the second end 41b of needle tubing 41 passes through first port 21 and thick needle 23 simultaneously
It stretches into vessel 11, the second rubber plug 43 is mounted in first port 21, and connector sleeve 42 is mounted on the third port 311 of injection tube 31
On；
Second of mounting means include：4th rubber plug 6 is mounted in connector sleeve 42, and the thick needle 23 of three-way connection 2 is inserted
The first rubber plug 12 is worn, the second end 41b of needle tubing 41 passes through first port 21 and thick needle 23 and stretches into vessel 11, the second rubber plug
43 are mounted in first port 21, and the third port 311 of injection tube 31 is mounted in second port 22.
The small blood plasma separating unit of richness used in the prior art, component forms more, complicated integral structure, cumbersome,
Delay the extraction rate of PRP, and needed to be shifted in multiple containers, increases platelet activation and contaminated machine
Meeting.The present invention need not replace container, and rich small blood blood plasma can be prepared merely with tool simple and easy to get.
To ensure syringe 3 no leakage, the third port 311 and second port 22 when drawing liquid, wherein
One of lateral wall on there is external screw thread, another madial wall to have the internal thread that coordinates with the external screw thread.In this way, utilizing spiral shell
The self-locking performance of line connection can ensure that second port 22 is bonded with third port 311.Due to three-way connection 2 and syringe 3
Usually plastic material also can guarantee the leakproofness of connection when second port 22 is connect with third port 311.
Similarly, the lateral wall of the third port 311 has external screw thread, has and institute on the madial wall of the connector sleeve 42
State the internal thread of external screw thread cooperation.In this way, can ensure that third port 311 is steady with connector sleeve 42 using the self-locking performance of threaded connection
It is solidly connected.Since syringe 3 and connector sleeve 42 are usually plastic material, the also company of can guarantee when syringe 3 and connector sleeve 42 connect
The leakproofness connect.
Visually blood 200 etc. in syringe 3, heparin tube 1 is filled in observation for convenience, and syringe 3 and heparin tube 1 are preferably
Transparent or semitransparent, the volume of syringe 3 and heparin tube 1 does not have specific limitation.Heparin tube 1 can be art technology routine
Heparin tube 1.Heparin tube 1 can be that blood 200, the blood 200 of acquisition are acquired directly from donor by blood taking needle when taking a blood sample
Usually whole blood can also be the blood platelet obtained from umbilical cord with blood or placenta, not be limited herein.
In view of the reliability of sealing, the first rubber plug 12, the second rubber plug 43, third rubber plug 5 and the 4th rubber plug 6 material
It can be the mechanograph of polypropylene or rubber (elastomer).In this way, the first rubber plug 12, the second rubber plug 43, third rubber plug 5 with
And the 4th rubber plug 6 be also convenient for disinfecting by gamma-rays.
The mounting means of the platelet rich plasma separator 100 at least there are two types of：
When the first mounting means, (f), (g) in Fig. 2 (a), (g), (h) and Fig. 3 are please referred to, third rubber plug 5 is mounted on
In second port 22, the thick needle 23 for the three-way connection 2 for being equipped with third rubber plug 5 is inserted through the first rubber plug 12, the of needle tubing 41
Two end 41b pass through first port 21 and thick needle 23 and stretch into vessel 11, and the second rubber plug 43 is mounted in first port 21, even
Female connector 42 is mounted in the third port 311 of injection tube 31.
Second of mounting means please refers in Fig. 3 (c), and the 4th rubber plug 6 is mounted in connector sleeve 42, by three-way connection 2
Thick needle 23 is inserted through the first rubber plug 12, and the second end 41b of needle tubing 41 passes through first port 21 and thick needle 23 and stretches into vessel 11
Interior, the second rubber plug 43 is mounted in first port 21, and the third port 311 of injection tube 31 is mounted in second port 22.
The separation method-of platelet rich plasma just sets method
Fig. 2 shows the flow diagrams of the separation method of the platelet rich plasma of second embodiment of the invention.It please refers to
The separation method of Fig. 2, the platelet rich plasma include the following steps：
Filling acquisition is had the heparin tube 1 of blood 200 just setting and carries out first time centrifugation, is separated by step S210
First part 210 containing red blood cell and the second part containing thrombocyte plasma 220.
The third rubber plug 5 is mounted in the second port 22 by step S220, and the thick needle 23 is inserted through described the
The second end 41b of one rubber plug 12, the needle tubing 41 passes through the first port 21 and the thick needle 23 and stretches into the vessel
11 bottom, second rubber plug 43 are mounted on the first port 21, and the connector sleeve 42 is mounted on the third port 311
On, first part 210 is sucked into the injection tube 31.
The syringe 3 for filling the first part 210 is separated the connector sleeve 42 by step S230, and described
4th rubber plug 6 is installed on connector sleeve 42, second of centrifugation to second part 220 of the heparin tube is sunken to described adopt
The bottom of blood vessel.
4th rubber plug 6 is separated the connector sleeve 42 by step S240, by the installation of the connector sleeve 42 to after emptying
In the third port 311 of the syringe 3, the needle tubing 41 is up-shifted to the upper layer position of the second part 220
It sets, the supernatant liquid of the second part 220 is sucked into the injection tube 31, remaining part is in the heparin tube 1
Rich small blood plate blood plasma.
In step S210, Fig. 2 (a) and Fig. 2 (b) are please referred to, the heparin tube of the blood 200 by the way that acquisition will be filled
1 carries out first time centrifugation, will contain the liquid of red blood cell and will be layered containing thrombocyte plasma.Illustration 2 (b) is small containing blood at the middle and upper levels
The second part 220 of plate blood plasma, lower layer are the first parts 210 containing red blood cell, and this is mainly due to contain thrombocyte plasma
Second part 220 density be less than the first part 210 containing red blood cell density.
First time centrifugation is carried out to the heparin tube 1 of the blood 200 of filling acquisition, the centrifugal force of centrifugation is for the first time
250g~350g, centrifugation time are 10min~20min.Due to red blood cell, the stem cell etc. contained in blood 200, if centrifugal force
It is excessive that be easy to cause stem cell etc. broken；And it is difficult to make blood 200 at second containing thrombocyte plasma that centrifugal force is too small
Divide 220 and the first part 210 containing red blood cell.Therefore the present invention sets centrifugal force as 250g~350g.In the prior art usually
It is setting centrifugal rotational speed, and the size of centrifugal force is related with radius R, wherein R is to centrifuge arbor center to horizontal centrifuge test tube
The distance of bottom or the distance in Vertical Centrifugal machine test tube mouth center.If being only selected centrifugal rotational speed, in centrifuge type
When variation or R change, centrifugal force then also changes therewith, and it is unstable to be easy to cause effect.The first time centrifugation that the present invention is arranged
Centrifugal force is preferably 300g, centrifugation time 15min.
In step S220, Fig. 2 (c) and Fig. 2 (d) are please referred to, third rubber plug 5 is mounted in the second port 22,
Due to band air filtering core on third rubber plug 5, three-way connection 2 is allow to be connected to outside air by air filtering core, and avoids sky
Gas is directly entered in three-way connection 2 and pollutes.It is inserted through first rubber plug 12 by thick needle 23, in this way, being convenient for needle tubing
41 extend into through the first port, thick needle 23 in the vessel 11 of heparin tube 1, while also may be used by the filtered air of air filtering core
It is suitable when ensureing to draw the liquid in heparin tube 1 to enter in the vessel 11 of heparin tube 1 via second port 22, thick needle 23
Smooth property.
The second end 41b of needle tubing 41 passes through the first port 21 and the thick needle 23 and the bottom for stretching into the vessel 11
Portion, in this way, the bottom of the second end 41b of needle tubing 41 reaches the bottom of the first part 210 containing red blood cell.Second rubber plug 43 is pacified
Mounted in the first port 21, to pass through 43 gas-tight seal first port 21 of the second rubber plug.The connector sleeve 42 is mounted on described
In third port 311, pass through gas-tight seal connector sleeve 42 and syringe 3.Since the first end 41a of needle tubing 41 is towards away from described the
The direction of two end 41b stretches out the connector sleeve 42, therefore, after the connector sleeve 42 is mounted in the third port 311, needle
The first end 41a of pipe 41 is connected to the injection tube 31 of syringe 3.It in this state, will when the piston of syringe 3 being pulled up
The first part 210 containing red blood cell in heparin tube 1 sucks in injection tube 31.
In step S230, Fig. 2 (e) and Fig. 2 (f) are please referred to, makes the syringe 3 of the filling first part 210
The connector sleeve 42 is separated, the syringe 3 for sucking the first part 210 containing red blood cell is unloaded, the of red blood cell will be contained
A part 210 moves to other containers, or discarded etc., is not limited herein.If desired it obtains purer containing thrombocyte plasma
Second part 220 then needs to carry out clearly syringe 3 after clearing up the first part 210 containing red blood cell in syringe 3
The processing, or the syringe 3 directly more renewed such as wash, sterilize in case being used in subsequent step.Syringe 3 separates connector sleeve 42
Afterwards, the 4th rubber plug 6 is installed on the connector sleeve 42, by gas-tight seal connector sleeve 42 and the 4th rubber plug 6, to avoid sky
Gas is directly entered heparin tube 1 and pollutes.Second of centrifugation to second part 220 of the heparin tube is sunken to the blood sampling
The bottom of pipe, in this way, subsequent operations are convenient in the bottom that the second part 220 containing blood platelet is sunken to heparin tube.Preferably, second
The centrifugal force of secondary centrifugation is 800g~1000g, centrifugation time 10min~20min.
Platelet rich plasma (PRP) is the blood plasma containing high concentration blood platelet obtained after being concentrated and separated blood 200, and
In second part 220 after second of centrifugal treating, the blood platelet being located in the liquid on upper layer is less, is located at lower liquid
The density of middle blood platelet is larger, therefore, it needs to come out the liquid assimilating on upper layer, makes only to be left the blood plasma of lower layer's enrichment blood platelet i.e.
For the platelet rich plasma (PRP) of needs.
In step S240, Fig. 2 (g), Fig. 2 (h) and Fig. 2 (j) are please referred to, the 4th rubber plug 6 is made to separate the connection
Set 42 is installed the connector sleeve 42 to the third port 311 of the syringe 3 after emptying, by gas-tight seal
The needle tubing 41 is up-shifted to the topside position of the second part 220 (in Fig. 2 by connector sleeve 42 and third port 311
(g) it is H, H values platelet content etc. in by the size of heparin tube 1, blood 200 to be promoted in the difference in height of 1 bottom of heparin tube
The influence of factor, therefore be not limited herein), the supernatant liquid of the second part 220 is sucked into the injection tube 31, blood sampling
Remaining part is rich small blood plate blood plasma in pipe 1.
Example 1
S1, will be filled with just setting and carrying out from the 10ml whole bloods to the heparin tube of 10ml taken with Healthy People for the first time from
The heart, centrifugal force 290g, centrifugation time 11min are separated into the first part 210 containing red blood cell and contain thrombocyte plasma
Second part 220.
Third rubber plug 5 is mounted in second port 22 by S2, and thick needle 23 is inserted through the first rubber plug 12, and the second of needle tubing 41
Hold 41b that first port 21 and thick needle 23 and the bottom for stretching into vessel 11, the second rubber plug 43 is passed through to be mounted on the first port
21, connector sleeve 42 is mounted in the third port 311, and first part 210 is sucked the injection tube 31；
The syringe 3 for filling first part 210 is separated connector sleeve 42, and installs the 4th rubber plug on connector sleeve 42 by S3
6, second of centrifugation of heparin tube is sunken to the bottom of heparin tube to the second part 220, the syringe 3 separated is handled
Liquid therein is emptied, or the syringe 3 more renewed is spare, the centrifugal force of second of centrifugation is 810g, centrifugation time 19min.
4th rubber plug 6 is separated connector sleeve 42 by S4, by the third port of syringe 3 of the installation of connector sleeve 42 to after emptying
On 311, needle tubing 41 is lifted up 2.5cm, the supernatant liquid of second part 220 is sucked into injection tube 31, retains bottom the
The two about 1ml of part 220, remaining part is rich small blood plate blood plasma in heparin tube 1.
Example 2
S1, will be filled with just setting and carrying out from the 10ml whole bloods to the heparin tube of 10ml taken with Healthy People for the first time from
The heart, centrifugal force 310g, centrifugation time 12min are separated into the first part 210 containing red blood cell and contain thrombocyte plasma
Second part 220.
Third rubber plug 5 is mounted in second port 22 by S2, and thick needle 23 is inserted through the first rubber plug 12, and the second of needle tubing 41
Hold 41b that first port 21 and thick needle 23 and the bottom for stretching into vessel 11, the second rubber plug 43 is passed through to be mounted on the first port
21, connector sleeve 42 is mounted in the third port 311, and first part 210 is sucked the injection tube 31；
The syringe 3 for filling first part 210 is separated connector sleeve 42, and installs the 4th rubber plug on connector sleeve 42 by S3
6, second of centrifugation of heparin tube is sunken to the bottom of heparin tube to the second part 220, the syringe 3 separated is handled
Liquid therein is emptied, or the syringe 3 more renewed is spare, the centrifugal force of second of centrifugation is 870g, centrifugation time 18min.
4th rubber plug 6 is separated connector sleeve 42 by S4, by the third port of syringe 3 of the installation of connector sleeve 42 to after emptying
On 311, needle tubing 41 is lifted up 2.7cm, the supernatant liquid of second part 220 is sucked into injection tube 31, retains bottom the
The two about 1.1ml of part 220, remaining part is rich small blood plate blood plasma in heparin tube 1.
Separation method-anastrophe of platelet rich plasma
Fig. 3 shows the flow diagram of the separation method of the platelet rich plasma of third embodiment of the invention.It please refers to
The separation method of Fig. 3, the platelet rich plasma include the following steps：
Filling acquisition is had the heparin tube 1 of blood 200 to be inverted and carries out first time centrifugation, is separated by step S310
First part 210 containing red blood cell and the second part containing thrombocyte plasma 220；
4th rubber plug 6 is mounted in the connector sleeve 42 by step S320, and the thick needle 23 is inserted through described first
The second end 41b of rubber plug 12, the needle tubing 41 passes through the first port 21 and the thick needle 23 and stretches into the vessel 11
Bottom, second rubber plug 43 be mounted on the first port 21, the third port 311 be mounted on the second port 22
On, first part 210 is sucked into the injection tube 31；
The syringe 3 for filling the first part 210 is separated the second port 22 by step S330, and in institute
It states and the third rubber plug 5 is installed in second port 22, the heparin tube is just installed to and carried out second of centrifugation, make described
Two parts 220 are sunken to the bottom of the heparin tube；
4th rubber plug 6 is separated the connector sleeve 42 by step S340, by the installation of the connector sleeve 42 to after emptying
In the third port 311 of the syringe 3, the needle tubing 41 is up-shifted to the upper layer position of the second part 220
It sets, the supernatant liquid of the second part 220 is sucked into the injection tube 31, remaining part is in the heparin tube 1
Rich small blood plate blood plasma.
In step S310, Fig. 3 (a) and Fig. 3 (b) are please referred to, the heparin tube of the blood 200 by the way that acquisition will be filled
First time centrifugation is carried out after 1 inversion, the liquid of red blood cell will be contained and is layered containing thrombocyte plasma.Illustration 3 (b) contains at the middle and upper levels
It is the first part 210 containing red blood cell to have the second part 220 of thrombocyte plasma, lower layer, and this is mainly due to small containing blood
The density of the second part 220 of plate blood plasma is less than the density of the first part 210 containing red blood cell.
First time centrifugation is carried out to the heparin tube 1 of the blood 200 of filling acquisition, the centrifugal force of centrifugation is for the first time
250g~350g, centrifugation time are 10min~20min.Due to red blood cell, the stem cell etc. contained in blood 200, if centrifugal force
It is excessive that be easy to cause stem cell etc. broken；And it is difficult to make blood 200 at second containing thrombocyte plasma that centrifugal force is too small
Divide 220 and the first part 210 containing red blood cell.Therefore the present invention sets centrifugal force as 250g~350g.In the prior art usually
It is setting centrifugal rotational speed, and the size of centrifugal force is related with radius R, wherein R is to centrifuge arbor center to horizontal centrifuge test tube
The distance of bottom or the distance in Vertical Centrifugal machine test tube mouth center.If being only selected centrifugal rotational speed, in centrifuge type
When variation or R change, centrifugal force then also changes therewith, and it is unstable to be easy to cause effect.The first time centrifugation that the present invention is arranged
Centrifugal force is preferably 300g, centrifugation time 15min.
In step s 320, Fig. 3 (c) is please referred to, the 4th rubber plug 6 is mounted in the connector sleeve 42, gas is passed through
Close sealed adapter sleeve 42 and the 4th rubber plug 6, thick needle 23 are inserted through first rubber plug 12, since inverted heparin tube 1 leans at this time
The liquid of nearly opening is the first part 210 containing red blood cell, therefore, thick needle 23 be inserted into after the first rubber plug 12 can with containing
There is the first part 210 of red blood cell to contact.
Meanwhile thick needle 23 is inserted through first rubber plug 12 and also allows for the second end 41b of the needle tubing 41 and passes through described the
Single port 21 and the thick needle 23 and the bottom for stretching into the vessel 11, second rubber plug 43 are mounted on the first port
21, with first port 21 and the second rubber plug 43 by gas-tight seal, at this point, air after filtering filter element filtering through needle tubing 41 into
Enter into heparin tube 1.The third port 311 is mounted in the second port 22, and syringe 3 is mounted on second port
On 22, in this way, liquid is sucked via thick needle 23, second port 22 in injection tube 31 convenient for syringe 3, by first part
210 suck the injection tube 31.Since the 4th rubber plug 6 carries air filtering core, can make can through the filtered air of air filtering core
To enter 11 bottom of vessel of heparin tube 1 via connector sleeve 42, first port 21, thick needle 23.
In step S330, Fig. 3 (d) and Fig. 3 (e) are please referred to, the syringe 3 of the first part 210 will be filled
The second port 22 is separated, and the third rubber plug 5 is installed in the second port 22, by the of sucking containing red blood cell
The syringe 3 of a part 210 unloads, and the first part 210 for containing red blood cell is moved to other containers, or discarded etc., herein not
It is limited.If desired obtain the purer second part 220 containing thrombocyte plasma, then need by syringe 3 containing red
Syringe 3 such as cleans, is sterilized at the processing in the first part 210 of cell after clearing up, or the syringe 3 directly more renewed with
It is used in standby subsequent step.The heparin tube is then just installed to and carried out second of centrifugation, keeps the second part 220 heavy
In the bottom of the heparin tube.
It is convenient for subsequent operations in the bottom that second part 220 containing blood platelet is sunken to heparin tube.Preferably, it centrifuges for second
Centrifugal force be 800g~1000g, centrifugation time 10min~20min.
Platelet rich plasma (PRP) is the blood plasma containing high concentration blood platelet obtained after being concentrated and separated blood 200, and
In second part 220 after second of centrifugal treating, the blood platelet being located in the liquid on upper layer is less, is located at lower liquid
The density of middle blood platelet is larger, therefore, it needs to come out the liquid assimilating on upper layer, makes only to be left the blood plasma of lower layer's enrichment blood platelet i.e.
For the platelet rich plasma (PRP) of needs.
In step S340, Fig. 3 (f), Fig. 2 (g) and Fig. 2 (h) are please referred to, the 4th rubber plug 6 is separated into the connection
Set 42 is installed the connector sleeve 42 to the third port 311 of the syringe 3 after emptying, by gas-tight seal
The needle tubing 41 is up-shifted to the topside position of the second part 220 (in Fig. 2 by connector sleeve 42 and third port 311
(g) it is H, H values platelet content etc. in by the size of heparin tube 1, blood 200 to be promoted in the difference in height of 1 bottom of heparin tube
The influence of factor, therefore be not limited herein), the supernatant liquid of the second part 220 is sucked into the injection tube 31, blood sampling
Remaining part is rich small blood plate blood plasma in pipe 1.
Example 3
S1, will be filled with from the 10ml whole bloods to the heparin tube of 10ml taken with Healthy People and be inverted progress for the first time from
The heart, centrifugal force 290g, centrifugation time 11min are separated into the first part 210 containing red blood cell and contain thrombocyte plasma
Second part 220.
4th rubber plug 6 is mounted in connector sleeve 42 by S2, and thick needle 23 is inserted through the first rubber plug 12, the second end of needle tubing 41
41b passes through first port 21 and thick needle 23 and the bottom for stretching into vessel 11, the second rubber plug 43 to be mounted on first port 21, third
Port 311 is mounted in second port 22, and first part 210 is sucked injection tube 31；
The syringe 3 for filling first part 210 is separated second port 22, and installs third in second port 22 by S3
Heparin tube is just installed and is carried out second of centrifugation, so that second part 220 is sunken to the bottom of heparin tube, to what is separated by rubber plug 5
Syringe 3 carries out processing and empties liquid therein, or the syringe 3 more renewed is spare, and the centrifugal force of second of centrifugation is 900g,
Centrifugation time 12min.
4th rubber plug 6 is separated connector sleeve 42 by S4, by the third port of syringe 3 of the installation of connector sleeve 42 to after emptying
On 311, needle tubing 41 is lifted up 2.5cm, the supernatant liquid of second part 220 is sucked into injection tube 31, retains bottom the
The two about 1ml of part 220, remaining part is rich small blood plate blood plasma in heparin tube 1.
Example 4
S1, will be filled with from the 10ml whole bloods to the heparin tube of 10ml taken with Healthy People and be inverted progress for the first time from
The heart, centrifugal force 320g, centrifugation time 13min are separated into the first part 210 containing red blood cell and contain thrombocyte plasma
Second part 220.
4th rubber plug 6 is mounted in connector sleeve 42 by S2, and thick needle 23 is inserted through the first rubber plug 12, the second end of needle tubing 41
41b passes through first port 21 and thick needle 23 and the bottom for stretching into vessel 11, the second rubber plug 43 to be mounted on first port 21, third
Port 311 is mounted in second port 22, and first part 210 is sucked injection tube 31；
The syringe 3 for filling first part 210 is separated second port 22, and installs third in second port 22 by S3
Heparin tube is just installed and is carried out second of centrifugation, so that second part 220 is sunken to the bottom of heparin tube, to what is separated by rubber plug 5
Syringe 3 carries out processing and empties liquid therein, or the syringe 3 more renewed is spare, and the centrifugal force of second of centrifugation is 850g,
Centrifugation time 15min.
4th rubber plug 6 is separated connector sleeve 42 by S4, by the third port of syringe 3 of the installation of connector sleeve 42 to after emptying
On 311, needle tubing 41 is lifted up 2.5cm, the supernatant liquid of second part 220 is sucked into injection tube 31, retains bottom the
The two about 1ml of part 220, remaining part is rich small blood plate blood plasma in heparin tube 1.
It these are only the preferred embodiment of the present invention, be not intended to limit the scope of the invention, it is every in the present invention
Inventive concept under, using equivalent structure transformation made by description of the invention and accompanying drawing content, or directly/be used in it indirectly
His relevant technical field is included in the scope of patent protection of the present invention.
Claims (9)
1. a kind of platelet rich plasma separator, which is characterized in that including：
There is heparin tube for acquiring blood opening up vessel and lid to close the first rubber plug of the opening；
Three-way connection has and is interconnected the first port being arranged, second port and can be inserted through the thick of first rubber plug
Syringe needle；
Syringe includes the injection tube with third port；
Long needle assemblies, including needle tubing, connector sleeve and the second rubber plug, the needle tubing have a first end and a second end, and described second
End is inserted through the connector sleeve and the setting of the second rubber plug successively, and the first end is described towards being stretched out away from the direction of the second end
Connector sleeve；And
Third rubber plug with air filtering core and the 4th rubber plug, the third rubber plug are selectively attached in the second port,
4th rubber plug is selectively attached in the connector sleeve；
Wherein, the platelet rich plasma separator has the first installation condition and the second installation condition, first installation
State includes：The third rubber plug is mounted in the second port, will be equipped with the three-way connection of the third rubber plug
Thick needle be inserted through the first rubber plug, the second end of needle tubing passes through first port and thick needle and stretches into vessel, the second rubber plug peace
In first port, connector sleeve is mounted in the third port of injection tube；
Second of mounting means include：4th rubber plug is mounted in connector sleeve, and the thick needle of three-way connection is inserted through the first rubber plug,
The second end of needle tubing passes through first port and thick needle and stretches into vessel, and the second rubber plug is mounted in first port, injection tube
Third port installation on the second port.
2. platelet rich plasma separator as described in claim 1, which is characterized in that the third port and described second
There is on the lateral wall of one of them external screw thread, another madial wall to have the internal thread coordinated with the external screw thread for port.
3. platelet rich plasma separator as described in claim 1, which is characterized in that the lateral wall of the third port has
There is external screw thread, there is the internal thread coordinated with the external screw thread on the madial wall of the connector sleeve.
4. a kind of separation method of platelet rich plasma, the separation method is used as described in claims 1 to 3 any one
Platelet rich plasma separator, which is characterized in that include the following steps：
There is the heparin tube of blood just setting filling acquisition and carry out first time centrifugation, is separated into first containing red blood cell
Point and the second part containing thrombocyte plasma；
The third rubber plug is mounted in the second port, the thick needle is inserted through first rubber plug, the needle tubing
Second end passes through the first port and the thick needle and the bottom for stretching into the vessel, second rubber plug to be mounted on described
First port, the connector sleeve are mounted in the third port, and first part is sucked the injection tube；
The syringe for filling the first part is separated into the connector sleeve, and installs the described 4th on the connector sleeve
Second of centrifugation of the heparin tube is sunken to the bottom of the heparin tube by rubber plug to the second part；
4th rubber plug is separated into the connector sleeve, the connector sleeve is installed to described of the syringe to emptying
On three ports, the needle tubing is up-shifted to the topside position of the second part, by the upper liquid of the second part
Body sucks the injection tube, and remaining part is rich small blood plate blood plasma in the heparin tube.
5. the separation method of platelet rich plasma as claimed in claim 4, which is characterized in that the centrifugation of the first time centrifugation
Power is 250g~350g, and centrifugation time is 10min~20min.
6. the separation method of platelet rich plasma as claimed in claim 4, which is characterized in that the centrifugal force of second centrifugation is
800g~1000g, centrifugation time are 10min~20min.
7. a kind of separation method of platelet rich plasma, the separation method is used as described in claims 1 to 3 any one
Platelet rich plasma separator, which is characterized in that include the following steps：
First time centrifugation is inverted and is carried out to the heparin tube of the blood of filling acquisition, is separated into first containing red blood cell
Point and the second part containing thrombocyte plasma；
4th rubber plug is mounted in the connector sleeve, the thick needle is inserted through first rubber plug, and the of the needle tubing
Two ends pass through the first port and the thick needle and the bottom for stretching into the vessel, and second rubber plug is mounted on described the
Single port, the third port are mounted in the second port, and first part is sucked the injection tube；
The syringe for filling the first part is separated into the second port, and in the second port described in installation
The heparin tube is just installed and is carried out second of centrifugation, the second part is made to be sunken to the heparin tube by third rubber plug
Bottom；
4th rubber plug is separated into the connector sleeve, the connector sleeve is installed to described of the syringe to emptying
On three ports, the needle tubing is up-shifted to the topside position of the second part, by the upper liquid of the second part
Body sucks the injection tube, and remaining part is rich small blood plate blood plasma in the heparin tube.
8. the separation method of platelet rich plasma as claimed in claim 7, which is characterized in that the centrifugation of the first time centrifugation
Power is 250g~350g, and centrifugation time is 10min~20min.
9. the separation method of platelet rich plasma as claimed in claim 7, which is characterized in that the centrifugal force of second centrifugation is
800g~1000g, centrifugation time are 10min~20min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI775179B (en) * | 2020-10-15 | 2022-08-21 | 華元生醫股份有限公司 | Kit for isolation of platelet-rich plasma and the method using the same |
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| CN106582908A (en) * | 2016-11-30 | 2017-04-26 | 深圳市达科为生物工程有限公司 | Platelet-rich plasma rapid separating device and method |
| CN106701548A (en) * | 2017-02-10 | 2017-05-24 | 柳州市妇幼保健院 | Lymphocyte extraction device and extraction method |
| CN107377238A (en) * | 2017-08-15 | 2017-11-24 | 维堪医美（厦门）科技有限公司 | A kind of platelet rich plasma separating extraction device |
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| WO2015108273A1 (en) * | 2014-01-15 | 2015-07-23 | 주식회사 퍼비스코리아 | Apparatus and method for separating blood for extraction of platelet rich plasma |
| CN105107233A (en) * | 2015-07-24 | 2015-12-02 | 上海市第六人民医院 | Preparation method and device for leukocyte-depleted platelet rich plasma |
| CN106139290A (en) * | 2016-08-01 | 2016-11-23 | 娄域峰 | Integration autologous platelet rich plasma preparation suit |
| CN106582908A (en) * | 2016-11-30 | 2017-04-26 | 深圳市达科为生物工程有限公司 | Platelet-rich plasma rapid separating device and method |
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