CN108551797A - A kind of processing method promoting corn hybrid seed seed vitality under drought stress - Google Patents
A kind of processing method promoting corn hybrid seed seed vitality under drought stress Download PDFInfo
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- CN108551797A CN108551797A CN201810127060.8A CN201810127060A CN108551797A CN 108551797 A CN108551797 A CN 108551797A CN 201810127060 A CN201810127060 A CN 201810127060A CN 108551797 A CN108551797 A CN 108551797A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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Abstract
A kind of processing method promoting corn hybrid seed seed vitality under drought stress of the present invention, comprises the following steps：1）Filter out full seed, the clean and tidy net seed in surface；2）Every liter, which is prepared, with distilled water contains 0.8 milligram of 1.6 milligrams of CuSO4·5H2O and 150 milligram of 350 milligrams of ZnSO4·7H2The seed mixture treatment fluid of O；3）Seed soaking：25 DEG C at room temperature, is soaked seed 8 10 hours with seed-soaking liquid；4）Seed dehydration：Seed after seed soaking is dried in 25 DEG C of constant temperature pneumatic floatings, makes the 25% water content sections of seed moisture content Hui Gan to 20.The beneficial effects of the invention are as follows：The requirement of this seed processing technology operating condition is low, and regulation is simple, easy to operate, of low cost, with obvious effects, disclosure satisfy that the requirement for the technology that mechanization is precisely sowed under drought stress.
Description
Technical field
The present invention relates to a kind of crop seed processing methods, are finely broadcast especially under drought stress to adapt to mechanization
Kind, promote the method for treating seeds of corn hybrid seed vigor.
Background technology
Corn is important in the world grain and forage crop, and average per unit area yield and total output occupy first place in the world.2014
Year starts, and China has surmounted the U.S., becomes the most maize sown area country in the whole world.Corn is very wide in distribution in China
It is general, northeast, North China and southwestern three areas are mainly distributed on, which forms a long and narrow corn plantings from northeast to southwest
The 90% of 85% and total output of Chinese maize total cultivated area has been concentrated in band, this plantation region.Arid is to influence corn life
Important Stress Factors are produced, corn growth is hindered, interferes its physiology course, seriously affect the yield of corn kernel.
Drought condition is one of stress that plant is most susceptible to and the very common environment stress factor of nature.
Arid can not only cause the internal structure of plant and the spatial variations of function, also restrict the height of the growth characters and yield of plant
It is low.
Current corn seed processing method is limited to seed pelleting mostly, and function is substantially limited to insect pest preventing and controlling.Current kind
Subprocessing method is difficult to solve two big outstanding problems：First, under drought stress conditions, it is difficult to effectively improve corn hybrid seed work
Power causes emergence difficult, Qi Miaonan；Second is that a small number of effectively broadcast preceding instant method for treating seeds, moisture is excessively high, it is difficult to meet machinery
Change the needs finely sowed, seed can not carry out Mechanization sowing after causing processing, and seed is difficult to store after processing.Maize production
In, there is an urgent need to find it is a kind of under drought stress environment it is effective and rapid, of low cost, be suitble to machine sowing, easily operated corn
Cenospecies processing method.
Invention content
The purpose of the present invention is under a kind of drought stress environment, can improve corn hybrid seed vigor, be suitable for mechanization essence
The method for treating seeds carefully sowed.Meet current arid area or corn seeding meets with the needs of dry season area maize production.For
This technical solution adopted by the present invention is that：
A kind of processing method promoting corn hybrid seed seed vitality under drought stress, comprises the following steps：
1）Seed screens：It is selected to corn seed progress, filter out full seed, the clean and tidy net seed in surface；
2）Prepare seed-soaking liquid：Every liter, which is prepared, with distilled water contains 0.8 milligram of -1.6 milligrams of CuSO4·5H2O and 150 milligram -350
Milligram ZnSO4·7H2The seed mixture treatment fluid of O；
3）Seed soaking：25 DEG C are at room temperature, soaked seed 8-10 hours with seed-soaking liquid, and seed-soaking liquid is advisable with not crossing 2-3 centimetres of the surface of the seed,
Period stirred seed 1 time every 1 hour, and treatment fluid is replenished in time according to seed soaking liquid level, keeps seed water suction uniform, reaches
Expanded state；
4）Seed dehydration：By step 3）Seed after seed soaking starts seed soaking to starting drying in room temperature moisturizing 14-16 hours
Time is 24 hours；Then, it is dried in 25 DEG C of constant temperature pneumatic floatings, makes seed moisture content Hui Gan to 20-25% water content sections.
Preferably, it prepares every liter with distilled water and contains 1.2 milligrams of CuSO4·5H2O and 250 milligram of ZnSO4·
7H2The seed mixture treatment fluid of O.
The beneficial effects of the invention are as follows：This seed processing technology operating condition requires low, regulation is simple, it is easy to operate, at
This is cheap, with obvious effects, disclosure satisfy that the requirement for the technology that mechanization is precisely sowed under drought stress.
Specific implementation mode
The present embodiment is No. four beautiful using 315, moral, 1302,506,207 5 corn hybrid seeds of agriculture star as experiment material, to go
Ionized water seed soaking is control group, is soaked seed using the Cu+Zn combined processing liquid of 5 kinds of different ratios, with the PEG-6000 of 18% concentration
Drought stress simulation environment.To 7 germinating energy of beautiful cenospecies, germination percentage, height of seedling, root long, radical, seedling stem weight and root dry weight fingers
Mark is analyzed, and reference is provided for trace element Cu, Zn most adaptation ratios influenced for Vigor of Zea mays L. Seeds, meanwhile, in field
The validity that optimum proportioning is verified under stress conditions improves seed and exists to excavate a kind of simple and effective seed processing technology
Emergence rate under drought stress, and then improve corn yield.
1 materials and methods
1.1 materials and reagent
5 different corn hybrid seeds：315, moral jade four, 1302,506, agriculture star 207.
Cu in treatment fluid2+Element derives from CuSO4·5H2O, Zn2+Element derives from ZnSO4·7H2O utilizes 18%
PEG-6000 solution Drought stress simulation environment.
1.2 experimental method
1.2.1 seed treatment
To each cenospecies, every part takes that kind of a skin cleaning, seed be complete, 50, seed of the same size, totally 6 parts, repeats three times.It is right
In 6 parts of seeds of each repetition, at 25 DEG C, (0mgCuSO is used respectively4+0mgZnSO4)/L、(0.4mgCuSO4+
50mgZnSO4)/L、(0.8mgCuSO4+150mgZnSO4)/L、(1.2mgCuSO4 + 250 mg ZnSO4)/L、
(1.6mgCuSO4+350mgZnSO4)/L、(2.0mgCuSO4+450mgZnSO4The CuSO of)/L concentration4And ZnSO4Solution impregnates 8-
10 hours.Seed after treatment fluid seed soaking, room temperature moisturizing 14-16 hours（Dry time that starts to soak seed-start is 24 small
When）.Then, 25 DEG C of constant temperature pneumatic floating dryings, make seed moisture content Hui Gan to 20-25%（Early-stage study result）Water content area
Section..
1.2.2 Seed germination
Seed is placed in bottom and is placed in the culture dish of three layers of filter paper after dry-cure being returned, and is placed in 25 DEG C of illumination boxs and sends out
Bud is tested, and during germination, is inspected periodically, is supplemented a concentration of 18% PEG-6000 solution to filter paper and be saturated (with 18% PEG-
6000 simulating drought environment).
1.2.3 index determining
Record 5% germination sets a number of days.It sets bed the 4th day, records chitting piece number, calculate germinating energy.7th day record hair
Bud seed number calculates germination percentage, while measuring the indexs such as height of seedling, root long, radical, seedling stem weight, root dry weight.
Height of seedling：Measure and record the height of seedling of each grain germination seed（Distance of the seed to blade tip）, calculate average
Number.
Root long：Measure and record the root long of the longest root of each grain germination seed（Seed to the end of longest root away from
From）, calculate average.
Radical：The radical of each grain germination seed is counted, average is calculated.
Seedling stem weight：The seedling of the chitting piece of same processing is cut along seed, is packed into paper bag together, 80 DEG C of drying 24 are small
When, until constant weight, after scales/electronic balance weighing dries sample, calculates the dry weight of every seed seedling.
Root dry weight：The root of the chitting piece of same processing is cut along seed, is packed into paper bag together, 80 DEG C of drying 24 are small
When, until constant weight, after scales/electronic balance weighing dries sample, calculates the dry weight of every seminal root.
2 results and analysis
Influence of the Cu+Zn combined processings of 2.1 different ratios to the germinating energy and germination percentage of seed under drought stress
Influence of the Cu+Zn combined processings of 1 different ratio of table to corn seed germination gesture (%) under drought stress
Influence of the Cu+Zn combined processings of 2 different ratio of table to corn seed germination rate (%) under drought stress
Under drought stress it can be seen from table 1,2, germinating energy, the germination percentage of 5 corn hybrid seeds have not compared with the control group
With the promotion of degree, and with the raising of Cu+Zn combined processing concentration proportionings in downward trend after first rising.
When the concentration proportioning of Cu+Zn combined processings is identical, the germinating energy of moral No. four this cenospecies of jade hybridizes than other
Kind is all low, but germination percentage is not much different in addition to control group.No matter with which kind of match Cu+Zn combined processings, 315,1302,506
The germinating energy of this 3 cenospecies can maintain 60% or more, and the germination percentage of 5 cenospecies can maintain 60% or more.5 miscellaneous
After handing over the Cu+Zn combined processings seed soaking in kind by different ratio, the germinating energy of cenospecies 315 is best, when Cu+Zn combined processings
Proportioning is（1.2+250）When mg/L, germinating energy is up to 93%, i.e., the seed number sprouted in each cenospecies in 4 days is most
Mostly is 315；Germinating energy is minimum for beautiful No. four of moral, when the proportioning of Cu+Zn combined processings is（1.2+250）Mg/L and（1.6+
350）It is up to side by side 77% when mg/L, illustrates beautiful No. four this cenospecies pair of moral（1.2+250）Mg/L~（1.6+350）
Mg/L Cu, the proportioning processing of Zn elements are insensitive.It is cenospecies 506 that germination percentage is highest after treatment, at the compound places Cu+Zn
The proportioning of reason is（1.2+250）When mg/L, germination percentage is up to 100%.It is more right after the processing of 315 and 506 the two cenospecies
It is 0%~20% according to the germinating energy of group, the amplification of germination percentage；1302 and 506 two cenospecies are no matter at the solution with which kind of concentration
Reason, they relative to control group are 0%~10% compared with the germinating energy amplification of control group, wherein 1302 average amplification are minimum, in Cu+Zn
The proportioning of combined processing is（1.2+250）It is 7% to have reached the cenospecies maximum amplification when mg/L；And 315 this hybridization
Amplification of the kind amplification compared to its control group than being higher than other two cenospecies, has been up to 18%；But these three hybridize
The germination percentage amplification of kind remains basically stable, and is 0%~17%.Germinating energy, the germination percentage of moral jade four and 207 two cenospecies of agriculture star
Although low compared to other three cenospecies, especially moral education four, germinating energy, germination percentage be all it is minimum in 5 cenospecies,
But the two cenospecies using after the Cu+Zn combined processings processing of different ratio germinating energy, germination percentage is compared with control group
More other three cenospecies of amplification all become apparent from, and have reached 10%~37%, and wherein moral education four is highest for amplification, in Cu+Zn
The proportioning of combined processing（1.2+250）Reach 37% when mg/L.
Compared with the corn hybrid seed of control group deionized water processing, 5 corn hybrid seeds in five kinds of different ratios
Under the processing of Cu+Zn combined processings, germinating energy, germination percentage have different degrees of raising, and not with Cu+Zn combined processings
With the raising of proportioning, downward trend after first rising is presented in germinating energy, germination percentage.And when Cu+Zn combined processings proportioning is
（1.2+250）When mg/L, germinating energy, the germination percentage of each corn hybrid seed reach highest.But it is compound when continuing growing Cu+Zn
When processing proportioning, the germinating energy of each cenospecies, germination percentage illustrates to use Cu+Zn combined processings not at downward trend
Matching processed corn seed germination gesture and germination percentage together, there are one be most adapted to ratio, and the compound places Cu+Zn of mistake high mixture ratio
Reason can also have adverse effect on corn.So when handling corn seed with Cu+Zn combined processings, use is most suitable
It is vital that proportioning, which goes processing,.
Influence of the Cu+Zn combined processings of 2.2 different ratios to seed height of seedling under drought stress
Influence of the Cu+Zn combined processings of 3 different ratio of table to corn seed average height of seedling (cm) under drought stress
As can be seen from Table 3, after drought stress 7 days, the height of seedling of control group is minimum；It is multiple with the Cu+Zn of different ratio
The height of seedling for closing treated corn hybrid seed has different degrees of raising.In a certain range, as Cu+Zn combined processings are matched
Growth trend is also presented in the raising of ratio, height of seedling, is in Cu+Zn combined processings proportioning（1.2+250）Mg/L treated maize seeds
The height of seedling longest of son, then when improving the proportioning of Cu+Zn combined processings, height of seedling just will present downward trend.
Under conditions of with identical proportioning Cu+Zn combined processings, other cenospecies of the growth fraction of 315 this cenospecies height of seedling
Will be poor, no matter with the Cu+Zn combined processing seeds of which kind of proportioning, height of seedling is all not above 1.0cm, with a concentration of
（1.2+250）Reach maximum when mg/L Cu+Zn combined processings, also only 0.89cm.And in other 4 cenospecies, height of seedling life
Maximum is 1302, is in Cu+Zn combined processings proportioning（1.2+250）Height of seedling when mg/L is 2.6cm；Moral jade No. four and agriculture
The height of seedling of 207 the two cenospecies of star is not as good as 1302 and 506；Its middle peasant's star 207 is compound in Cu+Zn to be minimum in 4 cenospecies
Processing matches（1.2+250）Reach up to 1.39cm when mg/L.It is handled using the Cu+Zn combined processings of different ratio
Afterwards, 315 and 207 the two cenospecies of agriculture star 3 groups other compared with the amplification ratio of control group it is low, wherein 315 with（1.2+
250）Reach maximum amplification after the Cu+Zn combined processings of mg/L proportionings and there was only 0.31cm, agriculture star 207 is slightly good, but maximum amplification
Only 0.91cm；And this 3 cenospecies amplification of moral jade four, 1302 and 506 will be apparently higher than 315 and agriculture star 207, wherein 1302
This cenospecies highest, Cu+Zn combined processings proportioning be（1.2+250）It is 1.96cm to reach maximum amplification when mg/L.
It is and right although the growth differences of height of seedling of 5 cenospecies after the Cu+Zn combined processings with same proportioning are different
Being compared according to group has different degrees of raising, and is in the proportioning of Cu+Zn combined processings（1.2+250）Height of seedling when mg/L is most
It is long, and all present and first increase downward trend again.To sum up show：Cu+Zn combined processing corn hybridizations are used under drought stress
Kind, can alleviate influence of the drought stress to corn hybridization seedling portion character to a certain extent, Cu+Zn combined processings it is suitable
Proportioning is（1.2+250）mg/L.
Influence of the Cu+Zn combined processings of 2.3 different ratios to the Analysing Root Characters of seed under drought stress
Influence of the Cu+Zn combined processings of 4 different ratio of table to average every corn seed root long (cm) under drought stress
Influence of the Cu+Zn combined processings of 5 different ratio of table to average every corn seed radical (item) under drought stress
As can be seen from Table 4, under drought stress, with the root long of 5 corn hybrid seeds after the Cu+Zn combined processings of different ratio
There is different degrees of promotion compared with the control group, and with the promotion of the proportioning of Cu+Zn combined processings under after first rising
The trend of drop.As can be seen from Table 5, in 5 corn hybrid seeds after the Cu+Zn combined processings of different ratio, 315 and agriculture star
The radical of 207 two cenospecies is compared with the control group without apparent gap, and the other three cenospecies gap is nor especially bright
It is aobvious, show to have no significant effect the radical of seedling with different ratio Cu+Zn combined processings.But there are radicals for different hybridization inter-species
Difference, such as：The radical of 1302 cenospecies only has 2, and the radical of 506 cenospecies has reached 4, the root of remaining cenospecies
Number is all at 3 or so.
When the proportioning of Cu+Zn combined processings is identical, the root long of cenospecies 315 is all lower than other cenospecies.No matter which is used
The Cu+Zn combined processings of kind of proportioning, moral education four, 1302,506 and the root longs of agriculture star 207 this 4 cenospecies can maintain
4.00cm or more, and 315 this cenospecies only exist in the proportioning of Cu+Zn combined processings（1.2+250）It is more than when mg/L
4.00cm（For 4.25cm）.In 5 cenospecies most by the root long of 506 cenospecies after the seed soaking of the Cu+Zn combined processings of different ratio
It is long, when the proportioning of Cu+Zn combined processings is（1.2+250）When mg/L, root long up to 8.16cm；Cenospecies 315 is located
The amplification of root long is minimum in 5 cenospecies after reason, no matter is handled with the Cu+Zn combined processings of which kind of proportioning, amplification only has
0.04~0.48cm；Remaining 4 cenospecies no matter with which kind of proportioning solution treatment, their amplification is compared with control group
It all increases by a fairly big margin, wherein 1302 average amplification highests, are in the proportioning of Cu+Zn combined processings（1.2+250）When mg/L
The maximum amplification 3.57cm of the cenospecies is reached；And 506 and the two the cenospecies amplification of agriculture star 207 compared to its control group ratio
To be less than the amplification of other two cenospecies, 207 minimum amplification of middle peasant's star only has 1.69cm；Although and 506 this cenospecies
No. four beautiful compared with control group amplification Bhide and 1302 it is low only have 2.47cm, but 506 are in the proportionings of Cu+Zn combined processings（1.2+
250）Reach longest root long in all cenospecies processing when mg/L.
Compared with the corn hybrid seed of control group deionized water processing, 5 corn hybrid seeds in 5 kinds of different ratio Cu+
Under Zn combined processings, root long has different degrees of raising, and with the raising that Cu+Zn combined processings match, and root long is in first
Situation is now increased, is in Cu+Zn combined processings proportioning（1.2+250）When mg/L, the root long of each corn hybrid seed reaches longest；
But when continuing growing Cu+Zn combined processings proportioning, the root long of each cenospecies does not continue to increase, but present
Downward trend.In conclusion under drought stress, Cu+Zn combined processings have growth of the corn hybrid seed in terms of root long aobvious
Effect is write, and is in Cu+Zn combined processings proportioning（1.2+250）Mg/L, which reaches, is most adapted to ratio.Regardless of the Cu+ of which kind of proportioning
Zn combined processings have little influence on the radical of corn hybrid seed, and the difference of radical is only related with cenospecies, this may be because of root
Number is controlled by genotype inherent cause, affected by environment smaller.
Influence of the Cu+Zn combined processings of 2.4 different ratios to the dry-matter accumulation amount of seed under drought stress
Under drought stress it can be seen from table 6, table 7, compared with the control group, after the Cu+Zn combined processings processing of different ratio
The dry-matter accumulation amounts of 5 corn hybrid seed seedling have a different degrees of increase, and present and first increase becoming of declining afterwards
Gesture, and increase and decrease rule in the same direction is presented with root dry matter in the seedling portion dry matter of corn hybrid seed.Corn hybrid seed is done after sprouting
Accumulation of the substance in root is higher than seedling portion.
Shadow of the Cu+Zn combined processings of 6 different ratio of table to average every corn seed seedling stem weight (g) under drought stress
It rings
Influence of the Cu+Zn combined processings of 7 different ratio of table to average every corn seed root dry weight (g) under drought stress
As can be seen from Table 6, when the proportioning of Cu+Zn combined processings is identical, the seedling portion dry matter of moral No. four this cenospecies of jade
Accumulation is most, reaches as high as 0.0230g；The seedling portion dry-matter accumulation amount of 315 this cenospecies is minimum, and highest only has
0.0078g.No matter the Cu+Zn combined processings matched with which kind of, the seedling portion dry-matter accumulation amount of 315 this cenospecies can not have
More than 0.0100g, exist in the proportioning of Cu+Zn combined processings（1.2+250）Its seedling portion dry-matter accumulation amount is maximum when mg/L
Only 0.0078g.315 and agriculture star 207 the two cenospecies handled after the amplification of seedling portion dry-matter accumulation amount be more other 3
Cenospecies is low, no matter is handled with the Cu+Zn combined processings of which kind of proportioning, seedling portion dry-matter accumulation amount amplification is all not above
0.0100g, only 0.0006~0.0097g exist wherein 315 is minimum in the proportioning of Cu+Zn combined processings（1.2+250）mg/L
When to reach maximum amplification be only 0.0029g.It is molten which kind of no matter this 3 cenospecies of moral jade four, 1302 and 506 matched with
Liquid processing, for their seedling portion dry-matter accumulation amount amplification compared with control group compared to all increasing by a fairly big margin, Sino-German beautiful No. four miscellaneous for 3
Amplification highest in kind is handed over, is in the proportioning of Cu+Zn combined processings（1.2+250）It is maximum the cenospecies has been reached when mg/L
Amplification is 0.0172g；And 1302 and 506 the two cenospecies, the amplification when the proportioning of Cu+Zn combined processings is identical are right compared with its
To be less than beautiful No. four amplification of moral according to group ratio is in the proportioning of Cu+Zn combined processings wherein 506 is minimum（1.2+250）Mg/L's
When reach maximum amplification and there was only 0.0103g.
As can be seen from Table 7, when the proportioning of Cu+Zn combined processings is identical, the root of moral No. four this cenospecies of jade is dry
Substance accumulation is most, reaches as high as 0.0310g；The root dry-matter accumulation amount of 207 this cenospecies of agriculture star is minimum, and highest is only
There is 0.0206g.No matter handled with the Cu+Zn combined processings of which kind of proportioning, 1302 and 207 the two cenospecies of agriculture star root it is dry
Substance accumulation can be not above 0.0250g, and 1302 root dry-matter accumulation amounts are big compared with agriculture star 207, in Cu+Zn combined processings
Proportioning exist（1.2+250）Its root dry-matter accumulation amount has been up to 0.0233g when mg/L.506 and agriculture star 207 this
Two cenospecies are that more other 3 cenospecies are low by the amplification of processing back root part dry-matter accumulation amount, and no matter middle peasant's star 207 is used
The Cu+Zn combined processings processing of which kind of proportioning, root dry-matter accumulation amount amplification are all not above 0.0100g, and highest only has
0.0086g, 506 this cenospecies exist in the proportioning of Cu+Zn combined processings（1.2+250）Amplification then reaches when mg/L
0.0124g.315, no matter moral beautiful No. four and 1302 this 3 cenospecies are with the solution treatment of which kind of concentration, their root dry
Matter accumulation amplification is compared compared with control group and is all increased by a fairly big margin, wherein 315 be amplification highest in 3 cenospecies, it is compound in Cu+Zn
The proportioning of processing is（1.2+250）It is 0.0176g to have reached the cenospecies maximum amplification when mg/L；And moral it is beautiful No. four and
1302 the two cenospecies, Cu+Zn combined processings proportioning it is identical when amplification compared with its control group than to be less than 315 increasing
Width is in the proportioning of Cu+Zn combined processings wherein 1302 is minimum（1.2+250）Reach maximum amplification when mg/L to only have
0.0150g。
With the increase that Cu+Zn combined processings match, seedling, the root dry-matter accumulation of corn hybrid seed are in first to increase to drop afterwards
The dry-matter accumulation of low trend, i.e., the increase matched with Cu+Zn combined processings, root and seedling is in rising trend, and in Cu+Zn
Combined processing proportioning reaches（1.2+250）When mg/L, effect is most apparent, is in Cu+Zn combined processings proportioning（1.2+250）
Mg/L~（2.0+450）Within the scope of mg/L times, with Cu+Zn combined processings match increase, each section dry-matter accumulation under
Drop.Show to can effectively improve the accumulation of seedling and root dry matter with different ratio Cu+Zn combined processing corn hybrid seeds,
But after being more than a certain amount of, promotion effect is not notable, this is issued with the corn hybrid seed after Cu+Zn combined processings in drought stress
The Resistence research result showed in terms of bud and growth is consistent.
Influence of the Cu+Zn combined processings of 2.5 different ratios to Seedling emergence rate under control water condition
The influence of Seedling emergence rate (%) under the 15% water content edaphic condition of Cu+Zn combined processings pair of 8 different ratio of table
The influence of Seedling emergence rate (%) under the 10% water content edaphic condition of Cu+Zn combined processings pair of 9 different ratio of table
The influence of Seedling emergence rate (%) under the 5% water content edaphic condition of Cu+Zn combined processings pair of 10 different ratio of table
To verify validity of this seed processing technology in terms of the drought-resistant side of body, experiment is by 5 cenospecies 15%, 10%, 5% 3
Optimum period, is planted under the conditions of kind soil moisture content, identifies its emergence rate.It can be seen from table 8,9,10 for each cenospecies,
As soil moisture stress degree is aggravated, the emergence rate respectively handled declines, but still with（1.2+250）The Cu+Zn of mg/L is compound
Treatment effect is best, emergence rate highest；For different cultivars, under 3 kinds of water stress, with（1.2+250）Mg/L's
The emergence rate highest of Cu+Zn combined processings, and as stress level is deepened, the emergence rate under the combined processing declines minimum.It says
It is bright（1.2+250）The Cu+Zn combined processings of mg/L can effectively improve the corn hybrid seed under extensive field drought stress conditions
Drought tolerance, improve Seedling emergence rate.
3 conclusions and discussion
One of an important factor for arid is as influence gene expression in plants, growth and development, yield and quality and distribution, serious limitation
Plantation and extension of the plant in more wide area.Plant is to the adaptability of arid not only with plant by the degree of arid
It is related, and more by the heredity of plant itself and regulating and controlling for gene.Therefore from plant, the drought resisting of plant is furtherd investigate
Mechanism, its known Characteristics of Drought improve the drought resisting drought-resistance ability of plant hybrid, and maximum journey by sowing pre-treatment to it
Degree improves the yield and quality of crop, and the drought-resistant crop hybrid kind of science selection and breeding has become domestic and international experts and scholars
The hot issue for paying special attention to and studying.
Under drought stress, corn seed absorbs water, and ratio is difficult under normal circumstances, this directly influences the hair of corn seed
Bud gesture, germination percentage, height of seedling, root long, seedling stem weight, root dry weight etc..This experimental study shows under drought stress environment, is matched with difference
The Cu+Zn combined processing corn hybrid seeds of ratio, in the different degrees of germinating energy for improving corn hybrid seed of germination period, germination
Rate promotes the growth indexes such as height of seedling under drought stress, root long and radical, and improves seedling portion and root dry-matter accumulation amount.
And with the increase that Cu+Zn combined processings match, the growth indexes such as germinating energy, germination percentage and dry-matter accumulation amount all present and first increase
The trend for adding reduction again, when Cu+Zn combined processings proportioning exists（1.2+250）When mg/L, treatment effect at this time is best.
This experiment shows that using Cu+Zn combined processings germinating energy, hair of the corn hybrid seed under drought stress can be improved
Bud rate.There is research, it was also found that under drought stress, Cu+Zn combined processings can improve the membrane permeability of seed, reduce Premeabilisation of cells
Stress improves germinating energy, the germination percentage of seed under drought stress to promote seed to sprout, and then improves the items of seed
Physical signs.This experimental study is, it was also found that treated that corn seed can improve it with the Cu+Zn combined processings suitably matched
The growth indexes such as height of seedling, root long and radical under drought stress, and increase seedling portion and root dry-matter accumulation amount.
Claims (2)
1. a kind of processing method promoting corn hybrid seed seed vitality under drought stress, which is characterized in that include following step
Suddenly：
1）Seed screens：It is selected to corn seed progress, filter out full seed, the clean and tidy net seed in surface；
2）Prepare seed-soaking liquid：Every liter, which is prepared, with distilled water contains 0.8 milligram of -1.6 milligrams of CuSO4·5H2O and 150 milligram of -350 milli
Gram ZnSO4·7H2The seed mixture treatment fluid of O；
3）Seed soaking：25 DEG C are at room temperature, soaked seed 8-10 hours with seed-soaking liquid, and seed-soaking liquid is advisable with not crossing 2-3 centimetres of the surface of the seed,
Period stirred seed 1 time every 1 hour, and treatment fluid is replenished in time according to seed soaking liquid level, keeps seed water suction uniform, reaches
Expanded state；
4）Seed dehydration：By step 3）Seed after seed soaking starts seed soaking to starting drying in room temperature moisturizing 14-16 hours
Time is 24 hours；Then, it is dried in 25 DEG C of constant temperature pneumatic floatings, makes seed moisture content Hui Gan to 20-25% water content sections.
2. a kind of processing method promoting corn hybrid seed seed vitality under drought stress according to claim 1,
It is characterized in that, preparing every liter with distilled water contains 1.2 milligrams of CuSO4·5H2O and 250 milligram of ZnSO4·7H2The seed mixture of O
Treatment fluid.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102612890A (en) * | 2012-04-09 | 2012-08-01 | 上海大学 | Method for improving vitality and cold resistance of corn seeds |
| CN103288549A (en) * | 2013-06-26 | 2013-09-11 | 周永珍 | Tobacco straw compound fertilizer for slowly releasing drought stress |
| WO2015109061A2 (en) * | 2014-01-15 | 2015-07-23 | New Biology, Inc. | Methods for improving germination and stress tolerance characteristics with jasmonates |
| CN104839160A (en) * | 2015-03-24 | 2015-08-19 | 安徽省农业科学院烟草研究所 | Drought-resistant maize soaking liquid and preparation method therefor |
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2018
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102612890A (en) * | 2012-04-09 | 2012-08-01 | 上海大学 | Method for improving vitality and cold resistance of corn seeds |
| CN103288549A (en) * | 2013-06-26 | 2013-09-11 | 周永珍 | Tobacco straw compound fertilizer for slowly releasing drought stress |
| WO2015109061A2 (en) * | 2014-01-15 | 2015-07-23 | New Biology, Inc. | Methods for improving germination and stress tolerance characteristics with jasmonates |
| CN104839160A (en) * | 2015-03-24 | 2015-08-19 | 安徽省农业科学院烟草研究所 | Drought-resistant maize soaking liquid and preparation method therefor |
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