CN108535497A - A kind of full-automatic miniature Chemiluminescence Apparatus - Google Patents
A kind of full-automatic miniature Chemiluminescence Apparatus Download PDFInfo
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- CN108535497A CN108535497A CN201810061126.8A CN201810061126A CN108535497A CN 108535497 A CN108535497 A CN 108535497A CN 201810061126 A CN201810061126 A CN 201810061126A CN 108535497 A CN108535497 A CN 108535497A
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- 230000007246 mechanism Effects 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 3
- 230000005622 photoelectricity Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 4
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 11
- 230000001276 controlling effect Effects 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
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- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
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- 210000004369 blood Anatomy 0.000 description 1
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- 238000012631 diagnostic technique Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00306—Housings, cabinets, control panels (details)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kind of full-automatic miniature Chemiluminescence Apparatus, including：It is set to the rotation control module of bottom of the frame, is used to convey kit thereon to operating position, and controls the shaft rotation of kit bottom；The piston drive module being set at the top of rotation control module, for driving the top piston for the kit for being located at operating position to move up and down；Magnetically attractive module positioned at operating position side, for adding magnetic to the magnetic bead in kit；And the photon detection module positioned at piston drive module side, photon intensity for detecting liquid are simultaneously fitted to concentration of specimens curve.The full-automatic miniature Chemiluminescence Apparatus of the present invention, reaction process can be allocated mixing and reaction by project regulated procedure to each component, it is automatically performed reagent detection, and can be realized simultaneously quick, the highly sensitive and quantitative detection to multiple projects, improves the reliability of testing result.
Description
Technical field
The present invention relates to a kind of chemiluminescence detector device more particularly to a kind of full-automatic miniature Chemiluminescence Apparatus.
Background technology
Occur various new biotechnologys with the development of the Human Genome Project, these biotechnologys are wide clinic
General application pushes and diagnoses market in accelerated the ripening emerging clinical diagnostic markets-molecule of developed country.Medical test circle is compeled
It is essential and fast and accurate detection means, Molecular Detection is wanted then to play unique advantage.The molecule diagnosis of broad sense includes clinical raw
Change and detection of nucleic acids are referred mainly at present using the DNA or RNA in various biotechnologys detection Organization individual, for diagnosing the illness,
Monitoring treatment or judging prognosis.
Molecular diagnostic techniques mainly have making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology at present.When
Before, the detection means such as blood routine, cytology, pathology and immunology are towards automation, integration, standardized direction
Development.But its own technical complexity of Molecular Detection, there is also many insoluble for the full-automatic instrument from sample to result
Technical problem.The nucleic acid extraction used in biotechnology at present will generally pass through cracking, combination, rinsing, elution process, then
In addition follow-up making nucleic acid molecular hybridization, polymerase chain reaction and biochip test step so that full-automatic from sample to result
It is very difficult to change instrument.List is for the configuration and addition of institute's extracting solution in each step and reaction solution, since final carries
When taking liquid and reaction solution to usually contain plurality of active ingredients, and being configured to the term of validity or effect after final extracting solution or reaction solution
Between it is very short, so market there is an urgent need to automatic configuration, add reagent and detection intelligent method.
The research and development of reagent, the automatic detection subsequently to be done, in addition to high-throughput mechanical arm flowing water are removed in IVD industries
Line keeps away not open the development of microflow control technique substantially.Microflow control technique is the technological means accurately manipulated to micro fluid, especially
Refer in sub-mm size rank.And micro-fluidic chip is then by the basic operation unit collection involved by biological and chemical field
On Cheng Yi block small chips, this chip is usually to be made of the microchannel network of various liquid storage tanks and interconnection, can be very
Big degree shortens the sample process time, and controls liquid flowing by precision, realizes the maximum utilization ratio of reagent consumptive material.
Invention content
The present invention is to solve the above problem in the prior art, proposes a kind of full-automatic miniature Chemiluminescence Apparatus.
To achieve the above object, the present invention uses following technical scheme：
The present invention provides a kind of full-automatic miniature Chemiluminescence Apparatus, includes mainly：
It is set to the rotation control module of bottom of the frame, is used to convey kit thereon to operating position, and control examination
The shaft in agent cassette bottom portion rotates, and drives each warehouse of kit rotatable connection；
The piston drive module being set at the top of the rotation control module, for driving the kit positioned at operating position
Top piston move up and down, extract or discharge in kit the indoor liquid in different storehouses；
Magnetically attractive module positioned at the operating position side draws liquid for adding magnetic to the magnetic bead in kit；And
Photon detection module positioned at piston drive module side, photon intensity for detecting liquid are simultaneously fitted to
Concentration of specimens curve.
Further, on the full-automatic miniature Chemiluminescence Apparatus, the rotation control module includes fixed frame, position
Electric rotating machine in the fixed frame bottom, and it is located at the enterprising export sliding rail of the fixed frame, kit pallet and rotation
Valve；The rotary valve be located in the middle part of the fixed frame and with the output axis connection of the electric rotating machine, the kit pallet
It is set to described on export sliding rail.
Further, on the full-automatic miniature Chemiluminescence Apparatus, the piston drive module includes being set to admittedly
Lead screw motor on fixed board and guiding mechanism, the output axis connection hitch gear of the lead screw motor, the hitch gear can be slided
Dynamic to be sheathed on the guiding mechanism, the guiding mechanism is equipped with locating stop piece.
Further, on the full-automatic miniature Chemiluminescence Apparatus, the magnet module includes guiding axis and removable
The dynamic fixed frame being arranged on the guiding axis, the fixed frame are equipped at least one magnet, and the magnet is solid by magnet
Fixed pole is fixed on the fixed frame.
Further, on the full-automatic miniature Chemiluminescence Apparatus, the photon detection module includes photomultiplier transit
Pipe, horizontal drive motor, photon counter, hidden door switch stepper motor, hidden door shielding plate and cam；Wherein, described laterally to drive
Dynamic motor is fixed on the rack for driving the photon detection block motion to designated position, the hidden door switch stepper motor
Shaft connect with the axle center of the cam, the cam is connect with the hidden door shielding plate, by the hidden door switch stepping electricity
Machine driving cam rotates, and hidden door shielding plate moves in a straight line and exits camera lens and block hole, to control the photoelectricity times
Increase pipe work.
It is further preferred that on the full-automatic miniature Chemiluminescence Apparatus, the hidden door shielding plate is slidably arranged in
On litter, one end of the litter is connected and fixed holder, and the other end connects black camera bellows block, the hidden door shielding plate with it is described black
Litter between color camera bellows block is equipped with spring, and the hidden door shielding plate offsets with the cam to be connect.
Further more preferably, further include slot type sensor and dress on the full-automatic miniature Chemiluminescence Apparatus
Catch on the hidden door shielding plate, the slot type sensor are fixed on the fixing bracket and black by sensor board
On camera bellows block, the motion control of the hidden door switch stepper motor blocks the slot type sensor by the catch.
Further, further include being located at the rotation control module one on the full-automatic miniature Chemiluminescence Apparatus
The warm bath module of side, for being heated to the agent bin in kit.
It is further preferred that on the full-automatic miniature Chemiluminescence Apparatus, the warm bath module include be set in
Heated for controlling temperature block, rack, gear on supporting rack and stepping decelerating motor；Described in the shaft connection of the stepping decelerating motor
Gear, the rack and the ratcheting connection of the rack, the heated for controlling temperature block are fixed on the rack, are slowed down by the stepping
Motor passes through the gear, rack drives heated for controlling temperature block to operating position.
Further, on the full-automatic miniature Chemiluminescence Apparatus, the magnetically attractive module is fixed on the rack,
Work is reached by the fixed frame band moving magnet in magnetically attractive module described in the gear, rack drives by the stepping decelerating motor
Position.
Kit of the present invention, including box main body and the shaft that is installed in the box main body, on the box main body
Several warehouses equipped with shaft storehouse and around shaft storehouse distribution, the warehouse bottom offers through-hole；The shaft by
Cavity, push rod and rubber plug form and are formed chamber, and the cavity is equipped with trepanning, the chamber by trepanning, through-hole with it is described
Warehouse is connected to, and the push rod upper end is equipped with the hook for being connect with the hitch gear in the rotation control module, the glue
Plug be fixed on the push rod bottom and the cavity bottom be equipped with for it is described rotation control module on rotary valve
The card slot of connection.
The present invention is had the following technical effect that compared with prior art using above-mentioned technical proposal：
Full-automatic miniature Chemiluminescence Apparatus provided by the invention detaches skill using Beads enrichment technology or oligonucleotide marker
The required component of detection process is placed in specific storehouse by art, antibody particle freeze drying technology and dedicated kit；Reaction process
Mixing and reaction are allocated to each component by project regulated procedure, entire test process is completed inside kit, no dirt
Dye, can be realized simultaneously quick, the highly sensitive and quantitative detection to multiple projects；Moreover, the light-emitting appearance can be automatically performed
Reagent detects, and operation is extremely intelligent, does not need manual intervention, improves the reliability of testing result, be effectively saved people
Power cost has higher application value.
Description of the drawings
Fig. 1 is the positive structure schematic of full-automatic miniature Chemiluminescence Apparatus of the present invention；
Fig. 2 is the back structures schematic diagram of full-automatic miniature Chemiluminescence Apparatus of the present invention；
Fig. 3 is the assembling schematic diagram of full-automatic miniature Chemiluminescence Apparatus of the present invention；
Fig. 4 is the structural schematic diagram that control module is rotated in full-automatic miniature Chemiluminescence Apparatus of the present invention；
Fig. 5 is the structural schematic diagram of piston drive module in full-automatic miniature Chemiluminescence Apparatus of the present invention；
Fig. 6 is the structural schematic diagram of warm bath module in full-automatic miniature Chemiluminescence Apparatus of the present invention；
Fig. 7 is the structural schematic diagram of magnetically attractive module in full-automatic miniature Chemiluminescence Apparatus of the present invention；
Fig. 8 is the positive structure schematic of photon detection module in full-automatic miniature Chemiluminescence Apparatus of the present invention；
Fig. 9 is the back structures schematic diagram of photon detection module in full-automatic miniature Chemiluminescence Apparatus of the present invention；
Figure 10 is the detonation configuration signal of photon detection module darkroom structure in full-automatic miniature Chemiluminescence Apparatus of the present invention
Figure；
Figure 11 is the present invention looks up structural representation of photon detection module darkroom structure shown in Figure 10；
Figure 12 is that Figure 11 hides the structural schematic diagram after black camera bellows block；
Figure 13 is the kit main structural components box main body and shaft for full-automatic miniature Chemiluminescence Apparatus of the present invention
Assembling schematic diagram；
Figure 14 is the structural schematic diagram for box main body in the kit of full-automatic miniature Chemiluminescence Apparatus of the present invention；
Figure 15 is the structural schematic diagram for shaft in the kit of full-automatic miniature Chemiluminescence Apparatus of the present invention；
Wherein, each reference numeral is：
10- rotates control module, and 11- electric rotating machines, 12- is into export sliding rail, 13- kit pallets, 14- rotary valves,
15- fixed frames, 20- piston drive modules, 21- lead screw motors, 22- guiding mechanisms, 23- hitch gears, 24- locating stop pieces, 25-
Fixed plate, 30- warm bath modules, 31- heated for controlling temperature blocks, 32- racks, 33- gears, 34- stepping decelerating motors, 35- supporting racks,
40- magnetically attractive modules, 41- guiding axis, 42- magnet, 43- magnet fixed links, 44- fixed frames, 50- photon detection modules, 51- photoelectricity
Multiplier tube, 52- horizontal drive motors, 53- photon counters, 54- hidden doors switch stepper motor, 55- hidden door shielding plates, and 56- drives
Moving cam, 57- fixing brackets, 58- catch, 59- litter, 60- springs, 61- black camera bellows blocks, 62- sensor boards, 63- slot types
Sensor, 64- camera lenses block hole, 70- box main bodies, 71- shafts storehouse, 72- warehouses, 73- through-holes, 80- shafts, 81- cavitys, 82-
Push rod, 83- rubber plugs, 84- chambers, 85- hooks, 86- trepannings, 87- card slots.
Specific implementation mode
The present invention is described in more detail below by specific embodiment, for a better understanding of the present invention,
But following embodiments are not intended to limit the scope of the invention.
As shown in Figs. 1-3, the present embodiment provides a kind of full-automatic miniature Chemiluminescence Apparatus, include mainly：It is set to rack
The rotation control module 10 of bottom is used to convey kit thereon to operating position, and controls the shaft 80 of kit bottom
Rotation drives kit to be rotatably connected each warehouse 72；It is set to the piston drive module 20 at 10 top of rotation control module,
For driving the top push rod 72 for the kit for being located at operating position to carry out piston motion up and down, extracting or discharging in kit not
With the liquid in warehouse 72；Magnetically attractive module 40 positioned at the operating position side is inhaled for adding magnetic to the magnetic bead in kit
Take liquid；And the photon detection module 50 positioned at 20 side of piston drive module, the photon intensity for detecting liquid
And it is fitted to concentration of specimens curve.
As illustrated in figs. 13-15, the present embodiment is used for the kit of full-automatic miniature Chemiluminescence Apparatus, including 70 He of box main body
The shaft 80 being installed in the box main body 70, the box main body 70 are equipped with shaft storehouse 71 and are distributed around the shaft storehouse 71
Several warehouses 72,72 bottom of the warehouse offers through-hole 73；The shaft 80 is by 83 groups of cavity 81, push rod 82 and rubber plug
At and form chamber 84, the cavity 81 is equipped with trepanning 86, and the chamber 84 passes through trepanning 86, through-hole 73 and the warehouse 72
Connection, 82 upper end of the push rod are equipped with the hook 85 for being connect with the hitch gear 23 in the rotation control module 10, institute
State rubber plug 83 be fixed on the push rod 82 bottom and the cavity 81 bottom be equipped with for the rotation control module
The card slot 87 that rotary valve 14 on 10 connects.
As shown in figure 4, the rotation control module 10 includes fixed frame 15, it is located at the electric rotating of 15 bottom of the fixed frame
Machine 11, and it is located at 15 enterprising export sliding rail 12 of the fixed frame, kit pallet 13 and rotary valve 14；The rotary valve 14
Positioned at the middle part of the fixed frame 15 and with the output axis connection of the electric rotating machine 11, the kit pallet 13 is set to institute
It states on export sliding rail 12.The rotation control module 10 into export sliding rail 12 by transporting kit into export, when kit arrives
Up to assigned work position, the shaft 80 that the electric rotating machine 11 of bottom drives rotary valve 14 to control kit bottom rotates, will not
It is connected to the chamber 84 by trepanning 86, through-hole 73 with warehouse 72, the control accuracy of rotary valve 14 relies primarily on electric rotating machine
The circuit board of retarder is carried on 11, which is reduced stepper motor and lost step phenomenon using 8 subdivision technologies.
As shown in figure 5, the piston drive module 20 includes lead screw motor 21 and the Guiding machine being set in fixed plate 25
Structure 22, the output axis connection hitch gear 23 of the lead screw motor 21, the hitch gear 23 is slidably sheathed on the guiding
In mechanism 22, the hook 85 in 23 kit of hitch gear snaps connection, and the guiding mechanism 22 is equipped with locating stop piece 24.When
When kit moves to operating position, hitch gear 23 catches on the top piston of kit, and driving lead screw motor 21 rotates, passes through
Guiding mechanism 22 makes hitch gear 23 move up and down, and motion control is blocked slot type sensor by locating stop piece 24 and (do not shown in figure
Go out) control.
As shown in Fig. 1-3,6, full-automatic miniature Chemiluminescence Apparatus further includes being located at 10 side of the rotation control module
Warm bath module 30, for being heated to the agent bin in kit.The warm bath module 30 includes being set in supporting rack 35
On heated for controlling temperature block 31, rack 32, gear 33 and stepping decelerating motor 34；The shaft of the stepping decelerating motor 34 connects
The gear 33, the gear 33 and 32 ratcheting connection of the rack, the heated for controlling temperature block 31 are fixed on the rack 32,
Drive heated for controlling temperature block 31 to operating position by the gear 33, rack 32 by the stepping decelerating motor 34.Heated for controlling temperature
Block 31 is heated to assigned temperature, and thermostatic in agent bin is made to drive gear 33, rack by stepping decelerating motor 34 at 37 °
32 drive warm bath moulds 30 are heated to operating position, and after warm bath, driving stepping decelerating motor 34 drives warm bath mould 30 to exit
Operating position.
In addition, the magnetically attractive module 40 is fixed on the rack 32, the tooth is passed through by the stepping decelerating motor 34
Wheel 33, rack 32 drive the fixed frame 44 in the magnetically attractive module 40 to reach operating position with moving magnet 42.
As shown in fig. 7, the magnet module 40 includes guiding axis 41 and is removably set on consolidating on the guiding axis 41
Determine frame 44, the fixed frame 44 is equipped at least one magnet 42, and the magnet 42 is fixed on described by magnet fixed link 43
On fixed frame 44.When needing magnet module 40 to work, the stepping decelerating motor 34 of warm bath module 30 drives gear 33, rack 32
Driving fixed frame 44, then band moving magnet 42 reaches operating position.
As Figure 8-9, the photon detection module 50 includes photomultiplier 51, horizontal drive motor 52, photon meter
Number device 53, hidden door switch stepper motor 54, hidden door shielding plate 55 and driving cam 56；Wherein, the horizontal drive motor 52 is solid
It is scheduled in rack for driving the photon detection module 50 to move to designated position, the hidden door switch stepper motor 54 turns
Axis is connect with the axle center of the cam 56, and the cam 56 is connect with the hidden door shielding plate 55, and stepping is switched by the hidden door
54 driving cam 56 of motor rotates, and hidden door shielding plate 55 moves in a straight line and exits camera lens and block hole 64, to control
The photomultiplier 51 works.
As shown in Figure 10, it is the exploded perspective view of the darkrooms PMT structure in photon detection module, the hidden door shielding plate 55 is slided
Dynamic to be arranged on litter 59, one end of the litter 59 is connected and fixed holder 57, and the other end connects black camera bellows block 61, described dark
Door shielding plate 55 and the black camera bellows block 61 between litter 59 be equipped with spring 60, the hidden door shielding plate 55 with it is described convex
Wheel 56 offsets connection.Darkroom protection is needed when photomultiplier 51 is not at work, cam 56 withstands hidden door shielding plate 55
The camera lens for blocking black camera bellows block 61 blocks hole 64, ensures that light is unable to direct irradiation photomultiplier 51.Work as photomultiplier
51 it is in running order when, hidden door switch stepper motor 54 rotate 180 °, so that 56 proximal end of cam is withstood hidden door shielding plate 55, hidden door
Shielding plate 55 can move backward under the effect of spring 60, and the camera lens of black camera bellows block 61 is made to block hole 64 and photomultiplier 51
Recipient is connected to.
Please continue to refer to Figure 10, which further includes slot type sensor 63 and is installed in described dark
Catch 58 on door shielding plate 55, the slot type sensor 63 are fixed on the fixing bracket 57 and black by sensor board 62
On camera bellows block 61, the motion control of the hidden door switch stepper motor 54 blocks the slot type sensor by the catch 58
63。
Further include the slot for being installed in the designated position in addition, in the full-automatic miniature Chemiluminescence Apparatus of the present embodiment
Formula sensor, the slot type sensor are connect with the horizontal drive motor 52, are transported for controlling the photon detection module 50
Row arrives the designated position.
Specifically, when photon detection module 50 needs work, horizontal drive motor 52 drives photon detection module 50 to transport
It moves to designated position, position positioning is by the detection of slot type sensor, and as depicted in figs. 11-12, hidden door switch stepper motor 54 drives convex
56 movement of wheel, makes hidden door shielding plate 55 remove the blocking position camera lens on black camera bellows block 61 and blocks hole 64, photomultiplier 51
It starts to work；When not working, hidden door switchs the movement of 54 driving cam 56 of stepper motor, and hidden door shielding plate 55 is made to return to black camera bellows
Blocking position camera lens on block 61 blocks hole 64, and photomultiplier 51 is stopped.
The present invention provide full-automatic miniature Chemiluminescence Apparatus operation principle be：
Kit has been added after sample and has been put on kit pallet 13, has driven kit to work into export sliding rail 12
Position, rotary valve 14 drives the card slot 87 of 80 bottom of kit shaft, while hitch gear 23 catches on kit top piston and pushes away
Bar 82；Drive the shaft 80 in kit to rotate by piston drive module 20, make the holes of the different warehouses 72 in kit with
The connection connection of chamber 84 in shaft 80, recycles lead screw motor 21 that push rod 82 is driven to move up and down, by the liquid in chamber 84
It extracts or is injected into each warehouse 72；After mixed liquor is transferred to reaction warehouse by completion, heated for controlling temperature block 31 is begun to warm up, and makes reagent
It is heated to 37 ° of warm bath, the stepping decelerating motor 34 of warm bath module 30 drives gear 33, rack 32 with moving magnet mould after the completion of warm bath
Then band moving magnet 42 reaches operating position magnetically attractive to fixed frame 16 on block 40.Last horizontal drive motor 52 drives photon detection
Module 50 moves to designated position, and position positioning is detected by slot type sensor；Hidden door switchs 54 driving cam 56 of stepper motor fortune
It is dynamic, so that hidden door shielding plate 55 is removed camera lens and block hole 64, photomultiplier 51 is started to work；When not working, hidden door switchs stepping
54 driving cam 56 of motor moves, and so that hidden door shielding plate 55 is returned to camera lens and is blocked hole 64 and shuts out the light, and photomultiplier 51 stops
Work.
Specific embodiments of the present invention are described in detail above, but it is intended only as example, the present invention is simultaneously unlimited
It is formed on particular embodiments described above.To those skilled in the art, it is any to the equivalent modifications that carry out of the present invention and
It substitutes also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by impartial conversion and
Modification, all should be contained within the scope of the invention.
Claims (10)
1. a kind of full-automatic miniature Chemiluminescence Apparatus, which is characterized in that including：
It is set to the rotation control module (10) of bottom of the frame, is used to convey kit thereon to operating position, and control examination
The shaft in agent cassette bottom portion rotates, and drives each warehouse of kit rotatable connection；
The piston drive module (20) being set at the top of the rotation control module (10), for driving the examination positioned at operating position
The top piston of agent box moves up and down, and extracts or discharge the indoor liquid in different storehouses in kit；
Magnetically attractive module (40) positioned at the operating position side draws liquid for adding magnetic to the magnetic bead in kit；And
Photon detection module (50) positioned at piston drive module (20) side, photon intensity for detecting liquid are simultaneously intended
Synthesize concentration of specimens curve.
2. full-automatic miniature Chemiluminescence Apparatus according to claim 1, which is characterized in that the rotation control module (10)
Including fixed frame (15), it is located at the electric rotating machine (11) of the fixed frame (15) bottom, and on the fixed frame (15)
Into export sliding rail (12), kit pallet (13) and rotary valve (14)；The rotary valve (14) is located at the fixed frame (15)
Middle part and with the output axis connection of the electric rotating machine (11), the kit pallet (13) is set to described into export sliding rail
(12) on.
3. full-automatic miniature Chemiluminescence Apparatus according to claim 1, which is characterized in that the piston drive module (20)
Including the lead screw motor (21) being set in fixed plate (25) and guiding mechanism (22), the output shaft of the lead screw motor (21) connects
Hitch gear (23) is connect, the hitch gear (23) is slidably sheathed on the guiding mechanism (22), the guiding mechanism
(22) it is equipped with locating stop piece (24).
4. full-automatic miniature Chemiluminescence Apparatus according to claim 1, which is characterized in that the magnet module (40) includes
Guiding axis (41) and the fixed frame (44) being removably set on the guiding axis (41), the fixed frame (44) are equipped at least
One magnet (42), the magnet (42) are fixed on by magnet fixed link (43) on the fixed frame (44).
5. full-automatic miniature Chemiluminescence Apparatus according to claim 1, which is characterized in that the photon detection module (50)
Including photomultiplier (51), horizontal drive motor (52), photon counter (53), hidden door switch stepper motor (54), hidden door
Shielding plate (55) and cam (56)；Wherein, the horizontal drive motor (52) is fixed on the rack for driving the photon to examine
It surveys module (50) and moves to designated position, the shaft of the hidden door switch stepper motor (54) connects with the axle center of the cam (56)
It connects, the cam (56) connect with the hidden door shielding plate (55), and stepper motor (54) driving cam is switched by the hidden door
(56) it rotates, and hidden door shielding plate (55) moves in a straight line and exits camera lens and block hole (64), to control the photoelectricity
Multiplier tube (51) works.
6. full-automatic miniature Chemiluminescence Apparatus according to claim 5, which is characterized in that the hidden door shielding plate (55) is sliding
Dynamic to be arranged on litter (59), one end of the litter (59) is connected and fixed holder (57), and the other end connects black camera bellows block
(61), the litter (59) between the hidden door shielding plate (55) and the black camera bellows block (61) is equipped with spring (60), described
Hidden door shielding plate (55) offsets with the cam (56) and connect.
7. full-automatic miniature Chemiluminescence Apparatus according to claim 6, which is characterized in that further include slot type sensor (63)
With the catch (58) being installed on the hidden door shielding plate (55), the slot type sensor (63) is fixed by sensor board (62)
On the fixing bracket (57) and black camera bellows block (61), the motion control of the hidden door switch stepper motor (54) relies on institute
It states catch (58) and blocks the slot type sensor (63).
8. full-automatic miniature Chemiluminescence Apparatus according to claim 1, which is characterized in that further include being located at the rotation to control
The warm bath module (30) of molding block (10) side, for being heated to the agent bin in kit.
9. full-automatic miniature Chemiluminescence Apparatus according to claim 8, which is characterized in that the warm bath module (30) includes
It is set in heated for controlling temperature block (31), rack (32), gear (33) and stepping decelerating motor (34) on supporting rack (35)；Institute
The shaft for stating stepping decelerating motor (34) connects the gear (33), the gear (33) and the rack (32) ratcheting connection,
The heated for controlling temperature block (31) is fixed on the rack (32), passes through the gear by the stepping decelerating motor (34)
(33), rack (32) drives heated for controlling temperature block (31) to operating position.
10. full-automatic miniature Chemiluminescence Apparatus according to claim 9, which is characterized in that the magnetically attractive module (40) is solid
It is scheduled on the rack (32), the suction is driven by the gear (33), rack (32) by the stepping decelerating motor (34)
Fixed frame (44) band moving magnet (42) in magnetic module (40) reaches operating position.
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| CN109847820A (en) * | 2019-04-18 | 2019-06-07 | 天津诺迈科技有限公司 | The pre-packaged device of micro-fluidic chip and application method |
| CN110538680A (en) * | 2019-08-19 | 2019-12-06 | 昆山汇先医药技术有限公司 | Micro-fluidic sample processing equipment |
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| CN108535497B (en) | 2024-08-16 |
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