CN108531416A - A kind of environment-friendly stalk leavening and preparation method thereof - Google Patents
A kind of environment-friendly stalk leavening and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of environment-friendly stalk leavening and preparation method thereof, the environment-friendly stalk leavening includes the raw material of following parts by weight：26 30 parts of bacillus subtilis, 21 25 parts of Trichoderma viride, 10 14 parts of saccharomyces cerevisiae, 10 14 parts of aspergillus niger, 15 parts of pectase, 58 parts of amylase, 13 parts of Li Shi Trichodermas, 5 10 parts of acid protease, 5 10 parts of neutral proteinase, 8 15 parts of arthrobacterium, 5 10 parts of cellulase, 13 parts of anti-aging agent, further include 13 parts of composite bacteria agent, the composite bacteria agent includes the raw material of following parts by weight：Low temperature produces 13 parts of cellulose mycophenolate bacterium solution, 12 parts of room temperature production cellulose mycophenolate bacterium solution, 12 parts of room temperature production amylomyces bacterium solution, 24 parts of room temperature production acetic acid bacterium solution, 13 parts of room temperature yeast liquid, 35 parts of zymoexciter.The present invention microbial inoculum can accelerate stalk Rapid Fermentation under cryogenic, and have good concerted reaction between the microorganism of the microbial inoculum, have efficient degradation property to the stalk for being difficult to degrade.
Description
Technical field
The present invention relates to stalk fermentation technical fields more particularly to a kind of environment-friendly stalk leavening and preparation method thereof.
Background technology
The photosynthetic product of crops has more than half to be present in stalk, and stalk is rich in nitrogen, phosphorus, potassium, calcium, magnesium and has
Machine object etc. is a kind of strategic standby renewable resource, and China's crop material is resourceful, enormous amount, wide variety, according to number
According to statistics, 700,000,000 tons of annual crop material quantum of output or more, wherein 2.3 hundred million tons of straw, 2.2 hundred million tons of maize straw, beans potato
Deng 300,000,000 tons, 30% or so of world's total straw amount is accounted for.Since stalk is not easy decomposition in a short time, stalk resource is caused not have for a long time
Have and is reasonably developed, it is most of all as life combustion in addition to being used for washer, pasture on a small quantity, partly being made compost for heap
Material is burnt up, and in the area based on planting industry, crop material has become the obstacle in production.So crop straw burning is throughout wide
Big rural area, thus causes the serious waste of environmental pollution and organic resource.According to statistics, the whole nation is used as the work of returning to the field and organic fertilizer
Object stalk only accounts for 20% or so of total amount, and burning accounts for 17%, and primary product is carbon dioxide after straw burning, carbon monoxide, and two
The pernicious gases such as sulfur oxide, these gases cause serious environmental pollution after entering air.There is " village in every harvest season rural area
Village lights a fire, and smolders everywhere " scene, crop straw burning do four big harms：First, waste of resource, causes large amount of organic that cannot return
Field；Second is that seriously polluting environment；Three often cause fire and traffic accident；Four destroy farmland ecosystem substance cycle, cause
Soil fertility declines, and for the above phenomenon, people are proposed a kind of environment-friendly stalk leavening to carry out fermentation process to stalk.
The prior art has the following disadvantages：Existing environment-friendly stalk leavening can not effectively improve macromolecular substances
Degradation efficiency, the cellulose and Lignin degradation rate in stalk are relatively low, are unable to reach so as to cause the nutritive value of straw feed
Desired effect or to reach expected nutritive value the time it takes long.
Invention content
The purpose of the present invention is to provide a kind of environment-friendly stalk leavenings and preparation method thereof, have and greatly improve macromolecular
The degradation efficiency of substance increases cellulose in stalk, Lignin degradation rate, accelerates the advantages of fermenting speed, solves existing
The nutritive value of straw feed is unable to reach desired effect or reaches the long problem of expected nutritive value the time it takes.
A kind of environment-friendly stalk leavening according to the ... of the embodiment of the present invention and preparation method thereof, the environment-friendly stalk leavening packet
Include the raw material of following parts by weight：26-30 parts of bacillus subtilis, 21-25 parts of Trichoderma viride, 10-14 parts of saccharomyces cerevisiae, aspergillus niger
10-14 parts, 1-5 parts of pectase, 5-8 parts of amylase, 1-3 parts of Li Shi Trichodermas, 5-10 parts of acid protease, neutral proteinase 5-
10 parts, 8-15 parts of arthrobacterium, 5-10 parts of cellulase.
Further include 1-3 parts of composite bacteria agent on the basis of said program.
On the basis of said program, the composite bacteria agent includes the raw material of following parts by weight：Low temperature produces cellulose mycophenolate bacterium solution
1-3 parts, room temperature production 1-2 part of cellulose mycophenolate bacterium solution, room temperature production 1-2 parts of amylomyces bacterium solution, room temperature produce 2-4 parts of acetic acid bacterium solution, room temperature
1-3 parts of yeast liquid, 3-5 parts of zymoexciter.
On the basis of said program, the zymoexciter includes cellulase activators, the cellulase activators by
Potassium nitrate, zinc chloride, calcium nitrate are with 2-3：1-2：The mixing ratio mixing composition of 1-2.
On the basis of said program, the anti-aging agent is p-phenylenediamine.
On the basis of said program, the preparation method of the composite bacteria agent includes the following steps：
S1：Low temperature production cellulose mycophenolate bacterium solution, room temperature cellulase-producing bacterium solution, room temperature are produced into amylase bacterium by above-mentioned parts by weight
Liquid, room temperature production acetic acid bacterium solution, room temperature yeast liquid are put into mixing agitator；
S2：The temperature in mixing agitator is controlled at 10 DEG C -15 DEG C with temperature controller, makes mixing agitator with 300-
The rotating speed of 400r/min stirs the mixed bacteria liquid 3-7min being put into；
S3：After the completion of stirring, zymoexciter is added according to above-mentioned parts by weight, then controls temperature at 11-14 DEG C, control is mixed
It closes blender and 1-3min is stirred with the rotating speed of 200-300r/min, obtain composite bacteria agent.
On the basis of said program, include the following steps：
1) it stocks up：Raw material for standby is taken by weight；
2) it is inoculated with：26-30 parts of bacillus subtilis, 8-15 parts of arthrobacterium are seeded on beef-protein medium,
21-25 parts of Trichoderma viride, 10-14 parts of aspergillus niger, 1-3 parts of Li Shi Trichodermas are seeded in potato culture, saccharomyces cerevisiae 10-14
Part is seeded in potato dextrose agar, and controls temperature at 10-20 DEG C, cultivates 20h-24h；
3) it mixes：The viable bacteria that culture obtains is mixed according to mass ratio, 1-5 parts of pectase, amylase 5-8 is then added
Part, 5-10 parts of acid protease, 5-10 parts of neutral proteinase, 5-10 parts of cellulase stir 10-20min, are then added compound
1-3 parts of microbial inoculum stirs 1-3min, you can obtain leavening.
The present invention has an advantageous effect in that compared with prior art：
1, it is added with composite bacteria agent in the environmentally friendly leavening of this kind, which can accelerate stalk quickly to send out under cryogenic
Ferment, and there is good concerted reaction between the microorganism of the microbial inoculum, there is efficient degradation property to the stalk for being difficult to degrade,
Microbial inoculum compared with the existing technology reduces the 56% stalk fermentation decomposed time, solves present in crops straw returning field
Decomposed slow, returning to the field hardly possible problem, and the composite bacteria agent can also improve the content of organic matter in soil, play land used and increase soil fertility
Effect；
2, this kind of environment-friendly stalk leavening is also added with anti-aging agent-p-phenylenediamine, and p-phenylenediamine can delay or inhibit old
Change process so that the shelf-life of this kind of leavening improves 60%, effectively avoids and is susceptible to because the leavening shelf-life is too short
The problem of leavening fails.
Specific implementation mode
For that can further appreciate that feature, technological means and the concrete function reached of the present invention, below with specific implementation
Mode is described in further detail the present invention.
Embodiment 1
A kind of environment-friendly stalk leavening is present embodiments provided, this kind of environment-friendly stalk leavening includes the original of following parts by weight
Material：26 parts of bacillus subtilis, 21 parts of Trichoderma viride, 10 parts of saccharomyces cerevisiae, 10 parts of aspergillus niger, 1 part of pectase, 5 parts of amylase,
1 part of Li Shi Trichodermas, 5 parts of acid protease, 5 parts of neutral proteinase, 8 parts of arthrobacterium, 5 parts of cellulase, 1 part of composite bacteria agent.
Wherein, composite bacteria agent includes the raw material of following parts by weight：Low temperature produces 1 part of cellulose mycophenolate bacterium solution, room temperature produces cellulose
1 part of mould liquid, 1 part of room temperature production amylomyces bacterium solution, 2 parts of room temperature production acetic acid bacterium solution, 1 part of room temperature yeast liquid, 3 parts of zymoexciter；
Zymoexciter includes cellulase activators, and cellulase activators are by potassium nitrate, zinc chloride, calcium nitrate with 2：1：1 mixing
It is formed than mixing, anti-aging agent is p-phenylenediamine.
A kind of environment-friendly stalk leavening and preparation method thereof, includes the following steps：
1) it stocks up：Raw material for standby is taken by weight；
2) it is inoculated with：26 parts of bacillus subtilis, 8 parts of arthrobacterium are seeded on beef-protein medium, green wood
Mould 21 parts, 10 parts of aspergillus niger, 1 part of Li Shi Trichodermas be seeded in potato culture, 10 parts of saccharomyces cerevisiae is seeded in potato Portugal
Grape sugar agar medium, and temperature is controlled at 10 DEG C, cultivate 20h；
3) it mixes：The viable bacteria that culture obtains is mixed according to mass ratio, then 1 part of pectase of addition, 5 parts of amylase,
5 parts of acid protease, 5 parts of neutral proteinase, 5 parts of cellulase stir 10min, and 1 part of composite bacteria agent, stirring is then added
1min, you can obtain leavening.
Embodiment 2
A kind of environment-friendly stalk leavening is present embodiments provided, this kind of environment-friendly stalk leavening includes the original of following parts by weight
Material：27 parts of bacillus subtilis, 22 parts of Trichoderma viride, 11 parts of saccharomyces cerevisiae, 11 parts of aspergillus niger, 2 parts of pectase, 6 parts of amylase,
2 parts of Li Shi Trichodermas, 6 parts of acid protease, 6 parts of neutral proteinase, 9 parts of arthrobacterium, 6 parts of cellulase, 2 parts of composite bacteria agent.
Wherein, composite bacteria agent includes the raw material of following parts by weight：Low temperature produces 2 parts of cellulose mycophenolate bacterium solution, room temperature produces cellulose
2 parts of mould liquid, 2 parts of room temperature production amylomyces bacterium solution, 3 parts of room temperature production acetic acid bacterium solution, 2 parts of room temperature yeast liquid, 4 parts of zymoexciter；
Zymoexciter includes cellulase activators, and cellulase activators are by potassium nitrate, zinc chloride, calcium nitrate with 3：2：2 mixing
It is formed than mixing, anti-aging agent is p-phenylenediamine.
A kind of environment-friendly stalk leavening and preparation method thereof, includes the following steps：
1) it stocks up：Raw material for standby is taken by weight；
2) it is inoculated with：27 parts of bacillus subtilis, 9 parts of arthrobacterium are seeded on beef-protein medium, green wood
Mould 22 parts, 11 parts of aspergillus niger, 2 parts of Li Shi Trichodermas be seeded in potato culture, 11 parts of saccharomyces cerevisiae is seeded in potato Portugal
Grape sugar agar medium, and temperature is controlled at 11 DEG C, cultivate 21h；
3) it mixes：The viable bacteria that culture obtains is mixed according to mass ratio, then 2 parts of pectase of addition, 6 parts of amylase,
6 parts of acid protease, 6 parts of neutral proteinase, 6 parts of cellulase stir 11min, and 2 parts of composite bacteria agent, stirring is then added
2min, you can obtain leavening.
Embodiment 3
A kind of environment-friendly stalk leavening is present embodiments provided, this kind of environment-friendly stalk leavening includes the original of following parts by weight
Material：28 parts of bacillus subtilis, 23 parts of Trichoderma viride, 12 parts of saccharomyces cerevisiae, 12 parts of aspergillus niger, 3 parts of pectase, 7 parts of amylase,
3 parts of Li Shi Trichodermas, 7 parts of acid protease, 7 parts of neutral proteinase, 10 parts of arthrobacterium, 7 parts of cellulase, 3 parts of composite bacteria agent.
Wherein, composite bacteria agent includes the raw material of following parts by weight：Low temperature produces 3 parts of cellulose mycophenolate bacterium solution, room temperature produces cellulose
2 parts of mould liquid, 2 parts of room temperature production amylomyces bacterium solution, 4 parts of room temperature production acetic acid bacterium solution, 3 parts of room temperature yeast liquid, 5 parts of zymoexciter；
Zymoexciter includes cellulase activators, and cellulase activators are by potassium nitrate, zinc chloride, calcium nitrate with 3：2：1 mixing
It is formed than mixing, anti-aging agent is p-phenylenediamine.
A kind of environment-friendly stalk leavening and preparation method thereof, includes the following steps：
1) it stocks up：Raw material for standby is taken by weight；
2) it is inoculated with：28 parts of bacillus subtilis, 10 parts of arthrobacterium are seeded on beef-protein medium, green wood
Mould 23 parts, 12 parts of aspergillus niger, 3 parts of Li Shi Trichodermas be seeded in potato culture, 12 parts of saccharomyces cerevisiae is seeded in potato Portugal
Grape sugar agar medium, and temperature is controlled at 12 DEG C, cultivate 22h；
3) it mixes：The viable bacteria that culture obtains is mixed according to mass ratio, then 3 parts of pectase of addition, 7 parts of amylase,
7 parts of acid protease, 7 parts of neutral proteinase, 7 parts of cellulase stir 12min, and 3 parts of composite bacteria agent, stirring is then added
3min, you can obtain leavening.
Embodiment 4
A kind of environment-friendly stalk leavening is present embodiments provided, this kind of environment-friendly stalk leavening includes the original of following parts by weight
Material：29 parts of bacillus subtilis, 24 parts of Trichoderma viride, 13 parts of saccharomyces cerevisiae, 13 parts of aspergillus niger, 4 parts of pectase, amylase 8 part,
3 parts of Li Shi Trichodermas, 8 parts of acid protease, 8 parts of neutral proteinase, 11 parts of arthrobacterium, 8 parts of cellulase, 3 parts of composite bacteria agent.
Wherein, composite bacteria agent includes the raw material of following parts by weight：Low temperature produces 3 parts of cellulose mycophenolate bacterium solution, room temperature produces cellulose
2 parts of mould liquid, 2 parts of room temperature production amylomyces bacterium solution, 4 parts of room temperature production acetic acid bacterium solution, 3 parts of room temperature yeast liquid, 5 parts of zymoexciter；
Zymoexciter includes cellulase activators, and cellulase activators are by potassium nitrate, zinc chloride, calcium nitrate with 2：2：1 mixing
It is formed than mixing, anti-aging agent is p-phenylenediamine.
A kind of environment-friendly stalk leavening and preparation method thereof, includes the following steps：
1) it stocks up：Raw material for standby is taken by weight；
2) it is inoculated with：29 parts of bacillus subtilis, 11 parts of arthrobacterium are seeded on beef-protein medium, green wood
Mould 24 parts, 13 parts of aspergillus niger, 3 parts of Li Shi Trichodermas be seeded in potato culture, 13 parts of saccharomyces cerevisiae is seeded in potato Portugal
Grape sugar agar medium, and temperature is controlled at 13 DEG C, cultivate 23h；
3) it mixes：The viable bacteria that culture obtains is mixed according to mass ratio, then 4 parts of pectase of addition, amylase 8 part,
8 parts of acid protease, 8 parts of neutral proteinase, 8 parts of cellulase stir 13min, and 3 parts of composite bacteria agent, stirring is then added
3min, you can obtain leavening.
Embodiment 5
A kind of environment-friendly stalk leavening is present embodiments provided, this kind of environment-friendly stalk leavening includes the original of following parts by weight
Material：30 parts of bacillus subtilis, 25 parts of Trichoderma viride, 10 parts of saccharomyces cerevisiae, 10 parts of aspergillus niger, 5 parts of pectase, 5 parts of amylase,
3 parts of Li Shi Trichodermas, 10 parts of acid protease, 5 parts of neutral proteinase, 15 parts of arthrobacterium, 10 parts of cellulase, composite bacteria agent 3
Part.
Wherein, composite bacteria agent includes the raw material of following parts by weight：Low temperature produces 1 part of cellulose mycophenolate bacterium solution, room temperature produces cellulose
1 part of mould liquid, 1 part of room temperature production amylomyces bacterium solution, 2 parts of room temperature production acetic acid bacterium solution, 1 part of room temperature yeast liquid, 3 parts of zymoexciter；
Zymoexciter includes cellulase activators, and cellulase activators are by potassium nitrate, zinc chloride, calcium nitrate with 3：1：2 mixing
It is formed than mixing, anti-aging agent is p-phenylenediamine.
A kind of environment-friendly stalk leavening and preparation method thereof, includes the following steps：
1) it stocks up：Raw material for standby is taken by weight；
2) it is inoculated with：30 parts of bacillus subtilis, 8-15 parts of arthrobacterium are seeded on beef-protein medium, green
25 parts of trichoderma, 10 parts of aspergillus niger, 3 parts of Li Shi Trichodermas are seeded in potato culture, and 10 parts of saccharomyces cerevisiae is seeded in potato
Glucose agar medium, and temperature is controlled at 20 DEG C, culture is for 24 hours；
3) it mixes：The viable bacteria that culture obtains is mixed according to mass ratio, then 5 parts of pectase of addition, 5 parts of amylase,
10 parts of acid protease, 5 parts of neutral proteinase, 10 parts of cellulase stir 20min, and 3 parts of composite bacteria agent, stirring is then added
3min, you can obtain leavening.
Reference examples
| Index | Shelf-life/year | The straw decomposing time/day | Content of organics |
| Embodiment 1 | 2.6 | 11 | It is high |
| Embodiment 2 | 2.7 | 12 | It is high |
| Embodiment 3 | 2.5 | 11 | It is high |
| Embodiment 4 | 2.5 | 11 | It is high |
| Embodiment 5 | 2.7 | 10 | It is high |
| Reference examples | 1.5 | 17 | Generally |
In conclusion this kind of environment-friendly stalk leavening can reduce the decomposed time of stalk 35%, can also greatly improve
The content of organic matter in soil, and relative to common fermentation agent, the shelf-life of this kind of leavening improves 73%.
Place is not described in detail by the present invention, is the known technology of those skilled in the art.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of environment-friendly stalk leavening, it is characterised in that：The environment-friendly stalk leavening includes the raw material of following parts by weight：It is withered
Careless bacillus 26-30 parts, 21-25 parts of Trichoderma viride, 10-14 parts of saccharomyces cerevisiae, 10-14 parts of aspergillus niger, 1-5 parts of pectase,
5-8 parts of amylase, 1-3 parts of Li Shi Trichodermas, 5-10 parts of acid protease, 5-10 parts of neutral proteinase, 8-15 parts of arthrobacterium, fibre
Plain 5-10 parts of the enzyme of dimension, 1-3 parts of anti-aging agent.
2. a kind of environment-friendly stalk leavening according to claim 1, it is characterised in that：It further include 1-3 parts of composite bacteria agent.
3. a kind of environment-friendly stalk leavening according to claim 2, it is characterised in that：The composite bacteria agent includes following heavy
Measure the raw material of part：Low temperature produces 1-3 parts of cellulose mycophenolate bacterium solution, 1-2 parts of cellulose mycophenolate bacterium solution of room temperature production, room temperature production amylomyces bacterium solution 1-
2 parts, room temperature production 2-4 parts of acetic acid bacterium solution, 1-3 parts of room temperature yeast liquid, 3-5 parts of zymoexciter.
4. a kind of environment-friendly stalk leavening according to claim 3, it is characterised in that：The zymoexciter includes cellulose
Zymoexciter, the cellulase activators are by potassium nitrate, zinc chloride, calcium nitrate with 2-3：1-2：The mixing ratio mixing group of 1-2
At.
5. a kind of environment-friendly stalk leavening according to claim 1, it is characterised in that：The anti-aging agent is p-phenylenediamine.
6. a kind of environment-friendly stalk leavening according to claim 2 or 4, it is characterised in that：The preparation of the composite bacteria agent
Method includes the following steps：
S1：By above-mentioned parts by weight by low temperature production cellulose mycophenolate bacterium solution, room temperature cellulase-producing bacterium solution, room temperature production amylase bacterium solution,
Room temperature production acetic acid bacterium solution, room temperature yeast liquid are put into mixing agitator；
S2：The temperature in mixing agitator is controlled at 10 DEG C -15 DEG C with temperature controller, makes mixing agitator with 300-
The rotating speed of 400r/min stirs the mixed bacteria liquid 3-7min being put into；
S3：After the completion of stirring, zymoexciter is added according to above-mentioned parts by weight, then controls temperature at 11-14 DEG C, control mixing is stirred
It mixes device and 1-3min is stirred with the rotating speed of 200-300r/min, obtain composite bacteria agent.
7. according to a kind of preparation method of environment-friendly stalk leavening of claim 1-5 any one of them, it is characterised in that：Including
Following steps：
1) it stocks up：Raw material for standby is taken by weight；
2) it is inoculated with：26-30 parts of bacillus subtilis, 8-15 parts of arthrobacterium are seeded on beef-protein medium, green
21-25 parts of trichoderma, 10-14 parts of aspergillus niger, 1-3 parts of Li Shi Trichodermas are seeded in potato culture, and 10-14 parts of saccharomyces cerevisiae connects
Kind controls temperature at 10-20 DEG C in potato dextrose agar, cultivates 20h-24h；
3) it mixes：The viable bacteria that culture obtains is mixed according to mass ratio, then 1-5 parts of pectase of addition, 5-8 parts of amylase,
5-10 parts of acid protease, 5-10 parts of neutral proteinase, 5-10 parts of cellulase stir 10-20min, compound bacteria are then added
1-3 parts of agent stirs 1-3min, you can obtain leavening.
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| CN109943559A (en) * | 2019-02-25 | 2019-06-28 | 车团结 | A kind of immobilization fermentation agent system and its preparation and application |
| CN110313540A (en) * | 2019-07-11 | 2019-10-11 | 陕西省生物农业研究所 | A kind of method of composite ferment and maize straw biological Huang storage preparation forage grass |
| CN111820104A (en) * | 2020-07-31 | 2020-10-27 | 哈尔滨尼亚农业有限公司 | Rice seedling raising substrate and preparation method thereof, organic acid soil and preparation method thereof |
| CN112457070A (en) * | 2020-11-18 | 2021-03-09 | 上海玖沁环保科技有限公司 | Environment-friendly biological agent for treating straws |
| CN115491915A (en) * | 2022-10-28 | 2022-12-20 | 齐鲁工业大学 | Method for pulping wheat straw by using composite microbial inoculum in cooperation with enzyme preparation and product |
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2018
- 2018-02-26 CN CN201810159797.8A patent/CN108531416A/en not_active Withdrawn
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943559A (en) * | 2019-02-25 | 2019-06-28 | 车团结 | A kind of immobilization fermentation agent system and its preparation and application |
| CN109943559B (en) * | 2019-02-25 | 2021-04-27 | 车团结 | Immobilized leavening agent system and preparation and application thereof |
| CN110313540A (en) * | 2019-07-11 | 2019-10-11 | 陕西省生物农业研究所 | A kind of method of composite ferment and maize straw biological Huang storage preparation forage grass |
| CN111820104A (en) * | 2020-07-31 | 2020-10-27 | 哈尔滨尼亚农业有限公司 | Rice seedling raising substrate and preparation method thereof, organic acid soil and preparation method thereof |
| CN112457070A (en) * | 2020-11-18 | 2021-03-09 | 上海玖沁环保科技有限公司 | Environment-friendly biological agent for treating straws |
| CN115491915A (en) * | 2022-10-28 | 2022-12-20 | 齐鲁工业大学 | Method for pulping wheat straw by using composite microbial inoculum in cooperation with enzyme preparation and product |
| CN115491915B (en) * | 2022-10-28 | 2023-09-26 | 齐鲁工业大学 | Method for pulping wheat straw by utilizing composite microbial inoculum and enzyme preparation and product |
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