CN108522859A - Application of the bile acid in preparing the feed addictive for Broiler chicks - Google Patents
Application of the bile acid in preparing the feed addictive for Broiler chicks Download PDFInfo
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Abstract
The present invention provides application of the bile acid in preparing the feed addictive for Broiler chicks.The present invention adds 60 ~ 80 mg/kg bile acids by the results show in 0 ~ 42 day daily ration, can be effectively improved the production performance and Slaughter of Broiler chicks.The serum uric acid level that 80 mg/kg bile acids can significantly reduce Broiler chicks was added in daily ration at 0 ~ 42 day.Daily ration adds 60 ~ 80 mg/kg bile acids, its duodenum road lipoproteinesterase and lipase active can be enhanced, inhibitory hormone sensitive lipase activity, this is beneficial to improve utilization of the Broiler chicks to fat, employing for itself body fat is reduced, so as to improve the growth performance of Broiler chicks.Bile acid of the present invention is added in feed can promote the synthesis of liver very low density lipoprotein, and fat is accelerated to be transported from liver to adipose tissue, reduce deposition of the fat in liver, can effectively prevent the generation of fatty liver and liver and gall syndrome, reduce death and culling rate.
Description
Technical field
The invention belongs to animal feed additive fields, and in particular to bile acid is preparing the feed addition for Broiler chicks
Application in agent.
Background technology
In recent years, energy feed raw material especially grease price was constantly soaring, how to improve the utilization rate of Dietary Energy,
The additive amount for reducing grease reduces the harm that high grease is brought with cost-effective, becomes emphasis of people's attention.Add in feed
Add the substance with emulsification function to promote grease emulsifying, gained universal acceptance to the digestion of grease to improve Broiler chicks,
Include commonly soybean lecithin, lecithin, fatty acid glyceride, monoglyceride, sucrose ester etc..According to the literature, lecithin conduct
Exogenous emulsifier improves production performance etc. and obtains certain effect to the digestibility of butter in raising feed,
But improve the digestibility effect unobvious of soya-bean oil, coconut oil and lard.
Invention content
Application of the goal of the invention of the present invention there is provided bile acid in preparing the feed addictive for Broiler chicks.This
Invention adds for 0~21 day, 22~42 days and 0~42 day using Broiler chicks as target animals, by Comparative experiments Broiler chicks in daily ration
Add influence of the different level bile acid to growth performance, Slaughter, blood parameters and alimentary canal enzyme activity etc., with evaluation
Validity of the bile acid to Broiler chicks.
For achieving the above object, the present invention is achieved using following technical proposals：
The present invention provides application of the bile acid in preparing the feed addictive for Broiler chicks.
Further：The bile acid includes following component：By quality ratio, hyocholic acid 5%-20%, hyodesoxycholic acid
55%-75% and chenodeoxycholic acid 10%-20%.
Further：The preparation method of the bile acid includes the following steps：
(1) saponification
It weighs the bile paste of crushing, is added in reaction kettle, sodium hydroxide solution is added, heat up and keep fluidized state 6-24h,
Saponification liquor is cooled to solid-liquid layering, supernatant is removed, solid is bile paste saponification product；
(2) it decolourizes
Add water into bile paste saponification product and heat, so that bile paste saponification product is completely dissolved, hydrogen peroxide is then added, stirs evenly
After react, after completion of the reaction, reaction solution is filtered to get filtrate；
(3) it is acidified
The filtrate is cooled to room temperature, is slowly added to hydrochloric acid solution, and stir, when solution ph is in 3-4, stops adding
Acid obtains white form of solid object at this time；
(4) it purifies
By the white form of solid object filter after, add water to rinse, it is to be filtered go out water detection be in neutrality for washing terminate；Incited somebody to action
White form of solid object after filter is placed in oven and dried, and obtains bile acid crude；
(5) it recrystallizes
Obtained bile acid crude is put into extractor, ethyl acetate stirring and dissolving is added, waits for that bile acid crude is basic
Dissolving is added anhydrous sodium sulfate dehydration, collects solution, solution is concentrated under reduced pressure, concentrate press filtration is obtained bile acid；
(6) dry
Obtained bile acid is put into baking oven, logical steam is dried in vacuo, and waits for that bile acid moisture is no more than 1%, then
To the bile acid.
Further：Bile acid additive amount in feed is 60mg/kg~80mg/kg, can significantly improve Broiler chicks
Activity of Digestive Enzymes in Intestine improves utilization rate of the Broiler chicks to fat.
Further：60mg/kg~80mg/kg bile acids are added in 0~42 day feed of Broiler chicks, can effectively improve meat
The production performance of chick.
Further：60mg/kg~80mg/kg bile acids are added in 0~42 day feed of Broiler chicks, can effectively improve meat
The Slaughter of chick.
Further：80mg/kg bile acids are added in 0~42 day feed of Broiler chicks, can significantly reduce the blood of Broiler chicks
Clear uric acid level.
Further：Middle addition 60mg/kg~80mg/kg bile acids, can enhance Broiler chicks enteron aisle in Broiler chicks feed
Digestive enzyme activity improves Broiler chicks to the utilization rate of fat, inhibits the secretion of HSL.
Compared with prior art, it advantages of the present invention and has the technical effect that：The present invention is by the results show 0~42
60~80mg/kg bile acids are added in its daily ration, can be effectively improved the production performance and Slaughter of Broiler chicks.At 0~42 day
The serum uric acid level that 80mg/kg bile acids can significantly reduce Broiler chicks is added in daily ration.Daily ration adds 60~80mg/kg bile
Acid can enhance its duodenum road lipoproteinesterase and lipase active, and inhibitory hormone sensitive lipase activity, this is beneficial to
Utilization of the Broiler chicks to fat is improved, employing for itself body fat is reduced, so as to improve the growth performance of Broiler chicks.
Bile acid of the present invention is added in feed can promote the synthesis of liver very low density lipoprotein, accelerate fat by
Liver is transported to adipose tissue, is reduced deposition of the fat in liver, can effectively be prevented the generation of fatty liver and liver and gall syndrome,
Reduce death and culling rate.
Specific implementation mode
Further detailed description is done to technical scheme of the present invention with reference to specific embodiment.
Experiment of the bile acid to Broiler chicks validity is added in 1,0~21 day daily ration of embodiment
1. material and method
1.1 tested material
Experiment bile acid is provided by Shandong Long Chang animal healths Co., Ltd, is specifically included：Hyocholic acid 8.00%, pig goes
Oxycholic acid 70.67%, chenodeoxycholic acid 19.61%.
1.2 experimental animals and design
Experiment selects 432 1 ages in days to be averaged original body mass as the public young bird of AA commercial generations Broiler chicks of 43.18g, is randomly divided into 4
A processing each handles 6 repetitions, each repeats 18 chickens, and control group daily ration is corn bean pulp type daily ration.Experiment is divided into 0~
21 days and 22~42 days two stages.4 processing groups are added respectively at growth early period (0~21d) in basal diet：0、40、
The bile acid of 60 and 80mg/kg.
1.3 experiment daily rations
Experiment uses corn-soybean meal basal diet, basal diet trophic level to be marked with reference to country of the People's Republic of China (PRC)
It is accurate《Fowl raising standard》2004 editions coordinate, composition and the trophic level such as table 1.1 of experimental basis daily ration.When ration formulation,
Bile acid carries out dilution step by step with mountain flour and amplifies, and after mixing, is then mixed with other raw materials.
1.1 basal diet of table forms and trophic level
a：It is provided to female kilogram of mixed feed：Vitamin A, 9000IU；Vitamin D3, 3000IU；Vitamin E, 24mg；Dimension
Raw element
K3, 1.8mg；Vitamin B1, 2.0mg；Riboflavin, 5.0mg；Vitamin B6, 3.0mg；Vitamin B12, 0.1mg；Cigarette
Acid,
40mg；Pantothenic acid, 15mg；Folic acid, 1.0mg；Biotin, 0.05mg；Choline chloride, 500mg；Iron, 80mg；Copper,
20mg；
Zinc, 90mg；Manganese, 80mg；Iodine, 0.35mg；Selenium, 0.35mg.
b：Except metabolic energy is calculated value, other data are measured value.
1.4 feeding management
It tests and was carried out in China Agricultural University's Animal Science And Technology respiratory chamber on 04 07th, 2011 to May 26, examination
It is 42 days to test the phase.Test chicken uses three layers of cage, is fed for dry mash, is freely eaten and drinks water.Daily illumination in 24 hours, experiment the
Temperature control in 1 week declines weekly 2 DEG C at 34~35 DEG C, and final temperature controls between 20~26 DEG C, and relative humidity 45%~
55%.It is daily to observe chicken group health and mental status according to Broiler chicks routine immunization program immunity and feeding management.
1.5 testing indexs and its method
1.5.1 growth performance
When on-test, the original body mass of Broiler chicks is weighed, at the end of 21 days and 42 days each raising stages, morning is on an empty stomach
It weighs and records feed consumption rate.Calculate 0~21 day, 22~42 days and 0~42 day average daily gain (ADG), average daily gain
(ADFI) and feedstuff-meat ratio (F: G).
1.5.2 Slaughter
The 42nd day of experiment, often processing take 6 chickens (each to repeat 1) to be butchered, measure slaughter traits, half respectively at random
Net thorax weight, complete net thorax weight, chest muscle, leg flesh and abdominal fat weight, calculate carcass rate, half net thorax rate, complete net thorax rate and chest muscle, leg flesh and
Abdominal fat.Indices calculation formula is as follows：
Dressing percentage=(slaughter traits/live-weight) × 100%；Half net thorax rate=(half net thorax weight/live-weight) × 100%；
Complete net thorax rate=(complete net thorax weight/live-weight) × 100%；Leg flesh rate=(leg flesh weight/complete net thorax weight) × 100%；Chest
Flesh rate=(chest muscle weight/complete net thorax weight) × 100%；Abdominal fat=(abdominal fat weight/complete net thorax weight) × 100%.
1.5.3 blood parameters
Respectively at experiment the 21st and 42 day, often processing took 6 chickens (each to repeat 1) at random, with vacuum blood collection tube heart
Blood sampling, centrifugation prepare blood serum sample, measure triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), ball
Albumen (GLB) and uric acid (UA).
1.5.4 alimentary canal enzyme activity
Respectively at experiment the 21st and 42 day, often processing took 6 chickens (each to repeat 1) to be butchered at random, acquisition 12
Duodenum 12, barnyard, ileum and liver.Liquid nitrogen flash freezer is used for the measurement of its enzyme activity.Enzyme activity index includes lipoproteinesterase (LPL), fat
Fat enzyme (LPS) and hormone-sensitive lipase (HSL).
1.6 statistical analysis
Test data carries out one-way analysis of variance, Duncan's multiple comparison analysis using SAS8.0 statistical softwares.P values are big
It is significant difference in 0.05.
2 results and analysis
2.1 growth performance
Different level bile acid is added in 0~21 day daily ration of Broiler chicks influences respectively such as table 1.2 growth performance.By table
1.2 only in 0~21 day daily ration as it can be seen that add 40~80mg/kg bile acids, and 42 days weight, full period raise rank between each processing group
Section average daily gain, average daily gain and feedstuff-meat ratio are not significantly different (P ＞ 0.05).It should be the result shows that in Broiler chicks 0
40~80mg/kg bile acids are added in~21 days daily rations does not influence the production performance of Broiler chicks.
Influence of the bile acid to broiler growth performance is added in 1.2 0~21 days daily rations of table1
Note：1Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2ADG：Average daily gain；ADFI：Average daily gain；F∶G：Feedstuff-meat ratio.
2.2 Slaughter
Different level bile acid is added in 0~21 day daily ration of Broiler chicks influences result respectively such as to its 42 days Slaughter
Table 1.3.By table 1.3 as it can be seen that adding 40~80mg/kg bile acids only in 0~21 day daily ration, Broiler chicks is each between each processing group
Item Slaughter index is without significant difference (P ＞ 0.05).Should the result shows that in 0~21 day daily ration of Broiler chicks addition 40~
80mg/kg bile acids do not influence the Slaughter of Broiler chicks.
Influence of the bile acid to 42 days Slaughters of Broiler chicks is added in 1.3 0~21 days daily rations of table
Note：Difference shows person (P ＜ 0.05) or extremely aobvious person (P ＜ 0.01) between bits mark different lowercases in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2.3 blood parameters
Different level bile acid is added in 0~21 day daily ration of Broiler chicks to its Broiler chicks 21 and 42 days blood parameters
Measurement result such as table 1.4.By table 1.4 as it can be seen that adding 40~80mg/kg bile acids, each processing group meat only in 0~21 day daily ration
Chick 21 and 42 days serum triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), globulin (GLB)
It is also all not significantly different (P ＞ 0.05) with uric acid (UA).Should the result shows that, in 0~21 day daily ration add 40~80mg/kg courages
When juice acid, blood parameters are not influenced.
Influence of the bile acid to Broiler chicks blood parameters is added in 1.4 0~21 days daily rations of table1
Note：1Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2TG：Measure triglycerides；HDL：High-density lipoprotein；LDL：Low-density lipoprotein；GLB：Globulin；UA：Urine
Acid.
2.4 alimentary canal enzyme activity
Different level bile acid is added in 0~21 day daily ration of Broiler chicks influenced result to its 21 days and 42 days alimentary canal enzyme activity
Respectively such as table 1.5.By table 1.5 as it can be seen that adding 40~80mg/kg bile acids only in 0~21 day daily ration, between 21 days each processing groups
Liver, each digestive enzyme of jejunum and ileum and duodenal HSL activity are not significantly different (P ＞ 0.05), but add
60 and 80mg/kg bile acid processing group Broiler chicks duodenum lipoproteinesterases (LPL) and lipase (LPS) activity is added significantly to carry
High (P ＜ 0.01)；The HSL and LPS of liver, each digestive enzyme of duodenum and ileum and jejunum live between 42 days each processing groups
Property be not significantly different (P ＞ 0.05), but feed daily ration addition 60 and 80mg/kg bile acids Broiler chicks 42 days jejunum LPL
Activity is significantly higher than control group (P ＜ 0.01).Test result shows that 60~80mg/kg bile acids are added in daily ration can enhance meat
Chick Activity of Digestive Enzymes in Intestine improves utilization rate of the Broiler chicks to fat.
Influence 1 of the bile acid to Broiler chicks alimentary canal enzyme activity is added in 1.5 0~21 days daily rations of table
Note：1Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2LPL：Lipoproteinesterase；LPS：Lipase；HSL：Hormone-sensitive lipase.
Experimental result in 0~21 day daily ration it is found that add 40~80mg/kg bile acids, to meat son through this embodiment
The production performance of chicken, 42 days Slaughters and blood parameters have no significant effect.Daily ration adds 60~80mg/kg bile
Acid can enhance enteron aisle lipoproteinesterase and lipase active, and then be conducive to improve utilization of the Broiler chicks to fat.
Bile acid is added in 2,22~42 days daily rations of embodiment to Broiler chicks efficiency evaluation
1 material and method
1.1 tested material
With embodiment 1.
1.2 experimental animals and design
Experiment selects 432 1 ages in days to be averaged original body mass as the public young bird of AA commercial generations Broiler chicks of 43.05g, is randomly divided into 4
A processing each handles 6 repetitions, each repeats 18 chickens, and control group daily ration is corn bean pulp type daily ration.Experiment is divided into 0~
21 days and 22~42 days two stages.4 processing groups are added respectively at Later growth (22~42d) in basal diet：0、40、
The bile acid of 60 and 80mg/kg.
1.3 experiment daily rations
With embodiment 1.
1.4 feeding management
With embodiment 1.
1.5 testing indexs and its method
1.5.1 growth performance
With embodiment 1.
1.5.2 Slaughter
With embodiment 1.
1.5.3 blood parameters
With embodiment 1.
1.5.4 alimentary canal enzyme activity
With embodiment 1.
1.6 statistical analysis
With embodiment 1.
2 results
2.1 growth performance
Different level bile acid is added in 22~42 days daily rations of Broiler chicks influences respectively such as table 2.1 growth performance.By
Table 2.1 as it can be seen that only added bile acid in daily ration at 22~42 days, average adopt day by average daily gain between reason group everywhere in 0~21 day
Appetite and feedstuff-meat ratio are not significantly different (P ＞ 0.05).Compared with other processing groups, add at 60 and 80mg/kg bile acids
Reason group Broiler chicks 22~42 days and average daily gain in 0~42 day significantly improve, and feedstuff-meat ratio significantly reduces (P ＜ 0.01).The result
Show that 60~80mg/kg bile acids are added in 22~42 days daily rations of Broiler chicks can be effectively improved the production performance of Broiler chicks.
Influence of the bile acid to broiler growth performance is added in 2.1 22~42 days daily rations of table
Note：1Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2ADG：Average daily gain；ADFI：Average daily gain；F∶G：Feedstuff-meat ratio.
2.2 Slaughter
Different level bile acid is added in 22~42 days daily rations of Broiler chicks influences result difference to its 42 days Slaughter
Such as table 2.2.By table 2.2 as it can be seen that compared with other daily ration processing groups, 60 and 80mg/kg bile acids, meat in 22~42 days daily rations
Dressing percentage, complete net thorax rate, half net thorax rate and leg flesh rate when chick 42 days are significantly increased, and abdominal fat significantly reduces (P ＜
0.01).This can effectively improve Broiler chicks the result shows that adding 60~80mg/kg bile acids in 22~42 days daily rations of Broiler chicks
Slaughter.
Influence of the bile acid to 42 days Slaughters of Broiler chicks is added in 2.2 22~42 days daily rations of table
Note：Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate that difference does not show person (P ＞ 0.05).
2.3 blood parameters
Different level bile acid is added in 22~42 days daily rations of Broiler chicks to refer to its Broiler chicks 21 and 42 days blood biochemicals
Mark measurement result such as table 2.3.By table 2.3 as it can be seen that only adding bile acid in daily ration at 22~42 days, each 21 He of processing group Broiler chicks
42 days serum TGs, HDL, LDL and GLB content are not significantly different (P ＞ 0.05)；Serum UA contents are not significantly different within 21 days
(P ＞ 0.05), and at 42 days, the serum UA contents of feeding addition 80mg/kg bile acid processing groups are substantially less than other processing groups
(P=0.01).Should the result shows that, only in 22~42 days daily rations adding 80mg/kg bile acids can significantly reduce the blood of Broiler chicks
Clear uric acid level, but other blood parameters are influenced little.
Influence of the bile acid to Broiler chicks blood parameters is added in 2.3 22~42 days daily rations of table1
Note：1Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2TG：Measure triglycerides；HDL：High-density lipoprotein；LDL：Low-density lipoprotein；GLB：Globulin；UA：Urine
Acid.
2.4 alimentary canal enzyme activity
Different level bile acid is added in 22~42 days daily rations of Broiler chicks influences result to its 21 and 42 days alimentary canal enzyme activity
Respectively such as table 2.4.By table 2.4 as it can be seen that adding 40~80mg/kg bile acids, 21 days each processing groups only in 22~42 days daily rations
Between liver, duodenum, jejunum and ileum each digestive enzyme activity be not significantly different (P ＞ 0.05)；42 days each processing groups
Between each digestive enzyme activity of liver, jejunum and ileum be not significantly different (P ＞ 0.05), compared with other processing groups, feeding
Daily ration addition 60mg/kg and 80mg/kg bile acid processing group Broiler chicks duodenum LPL and LPS activity significantly increases, and hormone is quick
Feeling lipase (HSL) activity significantly reduces (P ＜ 0.01).The result shows that adding 60mg/kg~80mg/kg bile acids in daily ration
Broiler chicks Activity of Digestive Enzymes in Intestine can be enhanced, utilization rate of the Broiler chicks to fat is improved, inhibit the secretion of HSL, reduce Broiler chicks
Itself body fat is employed, so as to improve the growth performance of Broiler chicks.
Influence of the bile acid to Broiler chicks alimentary canal enzyme activity is added in 2.4 22~42 days daily rations of table1
Note：1Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2LPL：Lipoproteinesterase；LPS：Lipase；HSL：Hormone-sensitive lipase.
The results show through this embodiment adds 60mg/kg~80mg/kg bile in 22~42 days daily rations
Acid, can be effectively improved 22~42 days of Broiler chicks and 0~42 day production performance and 42 days Slaughters.Only at 22~42 days
The serum uric acid level that 80mg/kg bile acids can significantly reduce Broiler chicks is added in daily ration.To other blood parameters without bright
Development is rung.Daily ration adds 60~80mg/kg bile acids, can enhance its duodenum road lipoproteinesterase and lipase active, presses down
Hormone-sensitive lipase activity processed, this is beneficial to improve utilization of the Broiler chicks to fat, reduces employing for itself body fat, to
Improve the growth performance of Broiler chicks.
Bile acid is added in 3,0~42 days daily rations of embodiment to Broiler chicks efficiency evaluation
1 material and method
1.1 tested material
With embodiment 1.
1.2 experimental animals and design
Experiment selects 432 1 ages in days to be averaged original body mass as the public young bird of AA commercial generations Broiler chicks of 43.34g, is randomly divided into 4
A processing each handles 6 repetitions, each repeats 18 chickens, and control group daily ration is corn bean pulp type daily ration.Experiment is divided into 0~
21 days and 22~42 days two stages.4 processing groups are added respectively at growth period (0~42d) in basal diet：0、40、60
With the bile acid of 80mg/kg.
1.3 experiment daily rations
With embodiment 1.
1.4 feeding management
With embodiment 1.
1.5 testing indexs and its method
1.5.1 growth performance
With embodiment 1.
1.5.2 Slaughter
With embodiment 1.
1.5.3 blood parameters
With embodiment 1.
1.5.4 alimentary canal enzyme activity
With embodiment 1.
1.6 statistical analysis
With embodiment 1.
2 results and analysis
2.1 growth performance
Different level bile acid is added in 0~42 day daily ration of Broiler chicks influences respectively such as table 3.1 growth performance.By table
3.1 as it can be seen that 0~42 day adds bile acid in daily ration, average daily gain between reason group everywhere in 0~21 day, average daily gain and
Feedstuff-meat ratio is not significantly different (P ＞ 0.05).Compared with other processing groups, 60 and 80mg/kg bile acid processing group meat is added
Chick 22~42 days and average daily gain in 0~42 day significantly improve, and feedstuff-meat ratio significantly reduces (P ＜ 0.01).Should the result shows that
60mg/kg~80mg/kg bile acids are added in 0~42 day daily ration of Broiler chicks can be effectively improved the production performance of Broiler chicks.
Influence 1 of 3.1 0~42 days daily ration addition different level bile acids of table to broiler growth performance
Note：1Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2ADG：Average daily gain；ADFI：Average daily gain；F∶G：Feedstuff-meat ratio.
2.2 Slaughter
Different level bile acid is added in 0~42 day daily ration of Broiler chicks influences result respectively such as to its 42 days Slaughter
Table 3.2.By table 3.2 as it can be seen that compared with other daily ration processing groups, the 60 and 80mg/kg bile acids in 0~42 day daily ration, meat son
Dressing percentage, complete net thorax rate, half net thorax rate and leg flesh rate when chicken 42 days are significantly increased, and abdominal fat significantly reduces (P ＜
0.01).This can effectively improve Broiler chicks the result shows that adding 60~80mg/kg bile acids in 0~42 day daily ration of Broiler chicks
Slaughter.
Influence of the bile acid to 42 days Slaughters of Broiler chicks is added in 3.2 0~42 days daily rations of table
Note：Difference shows person (P ＜ 0.05) or extremely aobvious person (P ＜ 0.01) between bits mark different lowercases in data of going together；
The bright letter of indicating or same letter indicate difference not significantly (P ＞ 0.05).
2.3 blood parameters
Different level bile acid is added in 0~42 day daily ration of Broiler chicks to its Broiler chicks 21 and 42 days blood parameters
Measurement result such as table 3.3.By table 3.3 as it can be seen that adding within 0~42 day bile acid in daily ration, each processing group Broiler chicks 21 and 42 days blood
Clear TG, HDL, LDL and GLB content is not significantly different (P ＞ 0.05)；Serum UA contents are not significantly different (P ＞ within 21 days
0.05), and at 42 days, the serum UA contents of feeding addition 80mg/kg bile acid processing groups are substantially less than other processing groups (P ＜
0.05).Should the result shows that, 80mg/kg bile acids are added in 0~42 day daily ration and can significantly reduce the serum uric acid water of Broiler chicks
It is flat, but other blood parameters are influenced little.
Influence of the bile acid to Broiler chicks blood parameters is added in 3.3 0~42 days daily rations of table1
Note：1Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2TG：Measure triglycerides；HDL：High-density lipoprotein；LDL：Low-density lipoprotein；GLB：Globulin；UA：Urine
Acid.
2.4 alimentary canal enzyme activity
Different level bile acid is added in 0~42 day daily ration of Broiler chicks influences result point to its 21 and 42 days alimentary canal enzyme activity
Not such as table 3.4.By table 3.4 as it can be seen that adding 40~80mg/kg bile acids in 0~42 day daily ration, liver between 21 days each processing groups
Each digestive ferment and duodenal HSL activity of dirty, jejunum and ileum are not significantly different (P ＞ 0.05), but add 60
It is significantly improved (P ＜ 0.01) with 80mg/kg bile acid processing group Broiler chicks duodenum LPL and LPS activity；42 days each processing groups
Between each digestive enzyme activity of liver, jejunum and ileum be not significantly different (P ＞ 0.05).Add 60 and 80mg/kg bile acids
Processing group Broiler chicks duodenum LPL and LPS activity significantly increases, and HSL activity significantly reduces (P ＜ 0.01).The result shows that day
60~80mg/kg bile acids are added in grain can enhance Broiler chicks duodenum road digestive enzyme activity, improve Broiler chicks to fat
Utilization rate inhibits the secretion of HSL, employing for Broiler chicks itself body fat is reduced, so as to improve the growth performance of Broiler chicks.
Influence of the bile acid to Broiler chicks alimentary canal enzyme activity is added in 3.4 0~42 days daily rations of table1
Note：1Significant difference (P ＜ 0.05) or extremely notable (P ＜ 0.01) between shoulder mark difference lowercase in data of going together；
Do not indicate that letter or same letter indicate difference not significantly (P ＞ 0.05).
2LPL：Lipoproteinesterase；LPS：Lipase；HSL：Hormone-sensitive lipase.
The present embodiment the results show：60~80mg/kg bile acids are added in 0~42 day daily ration, but can effectively be changed
22~42 days of kind Broiler chicks and 0~42 day production performance and 42 days Slaughters.80mg/ is added in 0~42d daily rations
Kg bile acids can significantly reduce the serum uric acid level of Broiler chicks.Other blood parameters are had no significant effect.Daily ration adds
60~80mg/kg bile acids can enhance its duodenum road lipoproteinesterase and lipase active, inhibitory hormone sensitive lipase
Activity, this is beneficial to improve utilization of the Broiler chicks to fat, employing for itself body fat is reduced, so as to improve the growth of Broiler chicks
Performance.
The repeated authentication experiment of embodiment 4, bile acid on 817 broiler productions
Experimental subjects：Gaotang Tian Zhaicun, 817 broiler chicken, 5450 plumages are randomly divided into two processing groups, blank control group (1450
Plumage) and test group (4000 plumage).
Experimental method：Same booth, two waterlines are respectively used to control group and test group.10 ages in days start to test (2014-
7-13) (see that suggestion makes 1 age in days into if it is to client), 48 ages in days terminate (2014-8-19), amount to 38 days.Test group Long Chang
Bile acid is drunk water by 150g/T water to off-test, control group blank control.
Experimental result：In detail record 817 broiler chicken of each group into young number, total amount of livestock for sale, deliver weight, feed intake, death and culling rate for sale.(chicken
Group's influenza virus infection, medication three days in 30 age in days.)
4 each group broiler chicken of table into young number, total amount of livestock for sale, deliver weight, feed intake, death and culling rate result for sale
Test group is can be seen that from result statistical form 4 compared with the control group, and feedstuff-meat ratio reduces by 0.04, and the rate of animals delivered to the slaughter-house improves
2.89%, increase economic efficiency at least 0.25 yuan/only (817 broiler chicken selling prices are 5.72 yuan/jin, and feed average price is about
1.7 yuan/jin, breast energy cost is 0.15 yuan /).
Bile acid is the main active of bile, and while chyle fat during intestines liver recycles, fat is formed with aliphatic acid
Dissolubility compound enables aliphatic acid permeable membrane to absorb, to complete to digest and absorb.Since intestines liver cycle is not one completely enclosed
Cycle, endogenous emulsifier, lipase hyposecretion, which will become, influences the biggest factor that digest and assimilate of feed fat.Feed
Middle addition bile acid can promote the synthesis of liver very low density lipoprotein, and fat is accelerated to be transported from liver to adipose tissue, reduce
Deposition of the fat in liver can effectively prevent the generation of fatty liver and liver and gall syndrome, reduce death and culling rate.
Bile acid is remarkably improved Broiler chicks by the comprehensive function in grease emulsifying, digestion and the three phases of absorption
To the utilization rate of grease.
1, it emulsifies
Bile acid intramolecular not only contains hydrophilic hydroxyl and carboxyl, but also contains lipophilic alkyl, therefore bile acid has
There is stronger surface-active, the surface tension between water-oil phase can be reduced, promote the emulsification of grease, expands grease and lipase
Contact area, in favor of the abundant digestion of grease.
2, lipase is activated, lipase active is enhanced
The prolipase of pancreatic secretion is changed into active lipase competence exertion effect in small enteral, its activation must
Must there are the participation of bile acid, bile acid that lipase can be made to be attached on the fat drips surface of triglycerides, to play catalytic action.
Pancreatic lipase plays a role optimal pH ranging from 8~9, and small intestine leading portion by hydrochloric acid in gastric juice influenced pH value be 6~
7, in such a case pancreatic lipase do not work substantially, only when pancreatic lipase and bile acid form compound, pancreas fat
The cap structure of enzyme is opened, and catalytic group is exposed.Meanwhile the space structure of pancreatic lipase changes, and is formed and is dredged
Aqueous channels enter convenient for hydrophobic substance inside pancreatic lipase, close to the catalytic group of lipase.
3, aliphatic acid is transported
In the absorption process of aliphatic acid, there are special identifying systems, only aliphatic acid and bile for intestinal epithelial cell
Hydrochlorate combination, which forms compound, to be identified by intestinal epithelial cell, complete to make aliphatic acid can smoothly enter into intestinal villi film
At the absorption process of aliphatic acid.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited；Although with reference to aforementioned reality
Applying example, invention is explained in detail, for those of ordinary skill in the art, still can be to aforementioned implementation
Technical solution recorded in example is modified or equivalent replacement of some of the technical features；And these are changed or replace
It changes, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (8)
1. application of the bile acid in preparing the feed addictive for Broiler chicks.
2. application of the bile acid according to claim 1 in preparing the feed addictive for Broiler chicks, feature exist
In：The bile acid includes following component：By quality ratio, hyocholic acid 5%-20%, hyodesoxycholic acid 55%-75% and chenodeoxycholic
Sour 10%-20%.
3. application of the bile acid according to claim 1 in preparing the feed addictive for Broiler chicks, feature exist
In：The preparation method of the bile acid includes the following steps：
（1）Saponification
The bile paste of crushing is weighed, is added in reaction kettle, sodium hydroxide solution is added, heats up and keeps fluidized state 6-24h, by soap
Change liquid and be cooled to solid-liquid layering, supernatant is removed, solid is bile paste saponification product；
（2）Decoloration
Add water into bile paste saponification product and heat, so that bile paste saponification product is completely dissolved, hydrogen peroxide is then added, it is anti-after stirring evenly
It answers, after completion of the reaction, reaction solution is filtered to get filtrate；
（3）Acidification
The filtrate is cooled to room temperature, is slowly added to hydrochloric acid solution, and stir, when solution ph is in 3-4, stops acid adding,
White form of solid object is obtained at this time；
（4）Purifying
By the white form of solid object filter after, add water to rinse, it is to be filtered go out water detection be in neutrality for washing terminate；After filtering
White form of solid object be placed in oven and dried, obtain bile acid crude；
（5）Recrystallization
Obtained bile acid crude is put into extractor, ethyl acetate stirring and dissolving is added, waits for that bile acid crude dissolves substantially,
Anhydrous sodium sulfate dehydration is added, collects solution, solution is concentrated under reduced pressure, concentrate press filtration is obtained into bile acid；
（6）It is dry
Obtained bile acid is put into baking oven, logical steam is dried in vacuo, and waits for that bile acid moisture is no more than 1%, then is obtained described
Bile acid.
4. application of the bile acid according to claim 1 in preparing the feed addictive for Broiler chicks, feature exist
In：Bile acid additive amount in feed is the mg/kg of 60 mg/kg ~ 80, can significantly improve Broiler chicks enteron aisle digestive enzyme
Property, improve utilization rate of the Broiler chicks to fat.
5. application of the bile acid according to claim 1 in preparing the feed addictive for Broiler chicks, feature exist
In：The mg/kg bile acids of 60 mg/kg ~ 80 are added in feed within 0 ~ 42 day, can effectively improve the productivity of Broiler chicks in Broiler chicks
Energy.
6. application of the bile acid according to claim 1 in preparing the feed addictive for Broiler chicks, feature exist
In：The mg/kg bile acids of 60 mg/kg ~ 80 are added in feed within 0 ~ 42 day, can effectively improve the butchering property of Broiler chicks in Broiler chicks
Energy.
7. application of the bile acid according to claim 1 in preparing the feed addictive for Broiler chicks, feature exist
In：80 mg/kg bile acids are added in feed within 0 ~ 42 day, can significantly reduce the serum uric acid level of Broiler chicks in Broiler chicks.
8. application of the bile acid according to claim 1 in preparing the feed addictive for Broiler chicks, feature exist
In：The middle addition mg/kg bile acids of 60 mg/kg ~ 80, can enhance Broiler chicks Activity of Digestive Enzymes in Intestine, carry in Broiler chicks feed
High Broiler chicks inhibit the secretion of HSL to the utilization rate of fat.
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| CN112280721A (en) * | 2020-11-19 | 2021-01-29 | 山东龙昌动物保健品有限公司 | Paenibacillus polymyxa LCP-01, compound microbial inoculum compounded with bile acid and application thereof |
| CN112401071A (en) * | 2020-11-19 | 2021-02-26 | 山东龙昌动物保健品有限公司 | Application of bile acid composite microbial inoculum in preparation of mutton sheep feed additive |
| CN112907386A (en) * | 2021-01-27 | 2021-06-04 | 江门市英海饲料有限公司 | Feed grease adding system |
| CN113383870A (en) * | 2021-04-20 | 2021-09-14 | 青岛尚德生物技术有限公司 | Micro-ecological preparation for treating necrotic enteritis of broiler chickens and application thereof |
| CN117044839A (en) * | 2023-10-11 | 2023-11-14 | 成都铁骑力士饲料有限公司 | Application of nutrition enhancer in improving color and redness of Sichuan black pork |
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| CN104041678A (en) * | 2014-05-28 | 2014-09-17 | 山东龙昌动物保健品有限公司 | Bile acid composition and preparation method thereof |
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Cited By (7)
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| CN112280721A (en) * | 2020-11-19 | 2021-01-29 | 山东龙昌动物保健品有限公司 | Paenibacillus polymyxa LCP-01, compound microbial inoculum compounded with bile acid and application thereof |
| CN112401071A (en) * | 2020-11-19 | 2021-02-26 | 山东龙昌动物保健品有限公司 | Application of bile acid composite microbial inoculum in preparation of mutton sheep feed additive |
| CN112401071B (en) * | 2020-11-19 | 2021-08-27 | 山东龙昌动物保健品有限公司 | Application of bile acid composite microbial inoculum in preparation of mutton sheep feed additive |
| CN112907386A (en) * | 2021-01-27 | 2021-06-04 | 江门市英海饲料有限公司 | Feed grease adding system |
| CN113383870A (en) * | 2021-04-20 | 2021-09-14 | 青岛尚德生物技术有限公司 | Micro-ecological preparation for treating necrotic enteritis of broiler chickens and application thereof |
| CN117044839A (en) * | 2023-10-11 | 2023-11-14 | 成都铁骑力士饲料有限公司 | Application of nutrition enhancer in improving color and redness of Sichuan black pork |
| CN117044839B (en) * | 2023-10-11 | 2024-01-23 | 成都铁骑力士饲料有限公司 | Application of nutrition enhancer in improving color and redness of Sichuan black pork |
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