CN108503425A - Improve the liquid fertilizer of Kiwi berry graft survival rate - Google Patents
Improve the liquid fertilizer of Kiwi berry graft survival rate Download PDFInfo
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- CN108503425A CN108503425A CN201810171532.XA CN201810171532A CN108503425A CN 108503425 A CN108503425 A CN 108503425A CN 201810171532 A CN201810171532 A CN 201810171532A CN 108503425 A CN108503425 A CN 108503425A
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- liquid fertilizer
- scion
- survival rate
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
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Abstract
The invention discloses a kind of liquid fertilizers improving Kiwi berry graft survival rate, and ingredient and its parts by weight include:2 ~ 4 parts of 8.2 ~ 18.6 parts of auximone, 20 ~ 40 parts of zymotic fluid, 0.001 ~ 0.004 part of sodium molybdate, 0.4 ~ 1.5 part of chelated iron, 1 ~ 4 part of citric acid and activated carbon.It has the beneficial effect that:Above-mentioned liquid fertilizer can effectively improve the content and activity of stock and scion binding site L phenylalanine lyases, to improve the compatibility of stock and scion, improve grafting success rate.Using liquid fertilizer of the present invention for a period of time after, in scion cell NR activity improve, to NO3 ‑The absorbability of N enhances, NH4 +N conversion metabolism is fast, reduces NH4 +The accumulation of N, while scion soluble sugar, proline, soluble protein content and expression can be improved, the enhancing of tune ability is oozed in scion, and salt tolerance significantly improves.
Description
Technical field
The present invention relates to plant planting field, specifically a kind of liquid fertilizer for improving Kiwi berry graft survival rate.
Background technology
Graft technology has pushed the development of agriculture and forestry, and the nursery stock especially faced in production of forestry takes root difficult problem,
As one of the great difficult problem of restriction forest development.By graft technology, using the root system of stock, not only solves this and ask
Topic improves survival rate and resists adverse circumstance ability, and makes scion Fast Growth, in some instances it may even be possible to generate desired variation
Type is to achieve many things at one stroke.Currently, graft technology develops to Nurse seed grafting, and has from traditional branch, bud grafting
Tissue cultures and graft technology are organically combined into the micrografting to be formed, application field is constantly widened, and is applied not only to expand and be planted
Species group quantity, and more apply in breed improvement, breeding of new variety, " the multiple children of veteran " etc., in theoretical guidance
Lower graft technology is continuously available innovation and development.
In recent years, still there is certain limitation in practice although having a small amount of wound in the market and repairing liquid,
The needs largely grafted and used are cannot be satisfied, survival rate is low low with utilization ratio after there is grafting, cannot play big
The effect of area application, the grafting of the especially wooden grass class, which cannot be satisfied, uses needs.
Invention content
It is an object of the invention to a kind of contents that stock and scion binding site L-phenylalanine lyase can be improved
And activity, accelerate the liquid fertilizer of calli induction and the raising Kiwi berry graft survival rate of differentiation.
To achieve the above object, the technical solution that the present invention takes is:Improve the liquid fertilizer of Kiwi berry graft survival rate, ingredient
And its parts by weight include:8.2 ~ 18.6 parts of auximone, 20 ~ 40 parts of zymotic fluid, 0.001 ~ 0.004 part of sodium molybdate, chelated iron
2 ~ 4 parts of 0.4 ~ 1.5 part, 1 ~ 4 part of citric acid and activated carbon.Above-mentioned liquid fertilizer can effective stimulus L-Phenylalanine Ammonia-Lyase Gene
PAL(Phenylalanine ammonia-lyase)Efficient transcription is improving stock and scion binding site L-phenylalanine
While the content of aminonialyase, moreover it is possible to the activity for improving L-phenylalanine lyase to a certain extent, to improve stock
With the compatibility of scion, grafting success rate is improved.Using liquid fertilizer of the present invention for a period of time after, in scion cell NR activity improve,
To NO3 -The absorbability of-N enhances, NH4 +- N conversion metabolism is fast, reduces NH4 +The accumulation of-N, while scion can be improved
The enhancing of tune ability is oozed in soluble sugar, proline, soluble protein content and expression, scion, and salt tolerance significantly improves.
Auximone ingredient and its parts by weight are 7 ~ 15 parts of heteroauxin, 1 ~ 3 part of zeatin and gibberellin 0.2 ~ 0.6
Part.
The preparation method of zymotic fluid is:By 10 ~ 30 parts of discarded animal bones, 50 ~ 70 parts of feces of livestock and poultry, 20 ~ 30 parts of stalks,
30 ~ 50 parts of useless syrup and 10 ~ 20 parts of durian shells are pulverized and mixed, and 2 ~ 3 parts are added after 8 ~ 19 parts of 5 ~ 10d of EM bacteria agent anaerobic fermentation are added
Papain and 2 ~ 3 parts of bromelains continue 10 ~ 20d of anaerobic fermentation, and 150 ~ 200 parts of water, fully mistake after reaction are added
Filter, the filtrate after concentration is zymotic fluid.Not only contain three kinds of nitrogen, phosphorus, potassium a great number of elements in liquid fertilizer of the present invention, also contains abundant
Weight element, trace element and various amino acid, can meet Kiwi berry grafting during, stock and scion binding site are thin
Nutriment needed for the differentiation of born of the same parents' fast breeding, accelerates calli induction and differentiation.
The preparation method of chelated iron is:By FeSO4It is dissolved in the deionized water of 70 ~ 80 times of quality, is uniformly dissolved;By EDTA
~ 2Na is dissolved in 25 ~ 30 times of deionized water, is uniformly dissolved;Above two solution is uniformly mixed, 30 ~ 45min is stirred, it can
Obtain chelated iron hydrate;By chelated iron hydrate, 60 ~ 75 DEG C of drying are placed in, chelated iron is obtained.
The preparation method of activated carbon is:It is solid that 2 ~ 4% silicate solids, 0.1 ~ 0.6% Strong oxdiative magnesium are added in coconut shell powder
Body and 0.2 ~ 0.8% manganese acetate, roast under limited oxygen condition, and calcination temperature variation is 100 ~ 120 DEG C of 10 ~ 15min of calcining;200~
220 DEG C of 10 ~ 15min of calcining;300 ~ 320 DEG C of 10 ~ 15min of calcining;380 ~ 400 DEG C of 100 ~ 120min of calcining;320 ~ 350 DEG C of calcinings
20~30min;200 ~ 220 DEG C of 20 ~ 30min of calcining;100 ~ 120 DEG C of 30 ~ 40min of calcining;It is cold in dry gas after roasting
But, activated carbon is obtained.Dry gas ingredient and its content are 80 ~ 90%N2With 10 ~ 20%HCl.Activated carbon table prepared by the present invention
Face is coarse, and pore structure is abundant, and hole inner wall adheres to a small amount of nano particle, and the arrangement of these particles disperses, is unordered.Activated carbon hole
It is largely hydroxyl also to have a large amount of active group, these active groups, and fraction is carboxyl, carbonyl etc..Above-mentioned active group
Group plays a key effect in the activity for improving L-phenylalanine lyase, but specific mechanism is still not clear.
Compared with the prior art, the advantages of the present invention are as follows:Liquid fertilizer of the present invention can effective stimulus L-phenylalanine solution ammonia
Enzyme gene PAL(Phenylalanine ammonia-lyase)Efficient transcription is improving stock and scion binding site L-benzene
While the content of alanine aminonialyase, moreover it is possible to the activity for improving L-phenylalanine lyase to a certain extent, to carry
The compatibility of high stock and scion improves grafting success rate.Using liquid fertilizer of the present invention for a period of time after, in scion cell NR live
Property improve, to NO3 -The absorbability of-N enhances, NH4 +- N conversion metabolism is fast, reduces NH4 +The accumulation of-N, while can
Scion soluble sugar, proline, soluble protein content and expression are improved, the enhancing of tune ability is oozed in scion, and salt tolerance obviously carries
It is high.
Specific implementation mode
The present invention program is described further below by embodiment:
Embodiment 1:
The liquid fertilizer of Kiwi berry graft survival rate is improved, ingredient and its parts by weight include:10.5 parts of auximone, zymotic fluid 32
Part, 0.003 part of sodium molybdate, 0.7 part of chelated iron, 2.7 parts of citric acid and 3.4 parts of activated carbon.Above-mentioned liquid fertilizer can effective stimulus L-
Phenylalanine Ammonia-Lyase Gene PAL(Phenylalanine ammonia-lyase)Efficient transcription is improving stock and scion knot
While closing the content of position L-phenylalanine lyase, moreover it is possible to improve L-phenylalanine lyase to a certain extent
Activity improves grafting success rate to improve the compatibility of stock and scion.Using liquid fertilizer of the present invention for a period of time after, scion
NR activity improves in cell, to NO3 -The absorbability of-N enhances, NH4 +- N conversion metabolism is fast, reduces NH4 +- N's
Accumulation, while scion soluble sugar, proline, soluble protein content and expression can be improved, the tune ability of oozing of scion enhances, resistance to
Salt significantly improves.
Auximone ingredient and its parts by weight are 0.5 part of 7.8 parts of heteroauxin, 2.2 parts of zeatin and gibberellin.
The preparation method of zymotic fluid is:By 22 parts of discarded animal bones, 58 parts of feces of livestock and poultry, 23 parts of stalks, 41 portions of useless sugar
Water and 15 parts of durian shells are pulverized and mixed, and 2.4 parts of papains and 2.6 parts of spinach are added after 12 parts of EM bacteria agent anaerobic fermentation 8d are added
Trailing plants protease continues anaerobic fermentation 17d, and 180 parts of water are added, are fully filtered after reaction, the filtrate after concentration is zymotic fluid.This
Not only contain three kinds of nitrogen, phosphorus, potassium a great number of elements in invention liquid fertilizer, also contains abundant weight element, trace element and various
Amino acid, during Kiwi berry grafting can be met, stock and the nutrients needed for the differentiation of scion binding site cell fast breeding
Matter accelerates calli induction and differentiation.
The preparation method of chelated iron is:By FeSO4It is dissolved in the deionized water of 76 times of quality, is uniformly dissolved;By EDTA ~
2Na is dissolved in 27 times of deionized water, is uniformly dissolved;Above two solution is uniformly mixed, 38min is stirred, can must chelate
Iron hydrate;By chelated iron hydrate, 68 DEG C of drying are placed in, chelated iron is obtained.
The preparation method of activated carbon is:Be added in coconut shell powder 3% silicate solid, 0.4% Strong oxdiative magnesium solid and
0.6% manganese acetate roasts under limited oxygen condition, and calcination temperature variation is 100 DEG C of calcining 15min;210 DEG C of calcining 13min;300℃
Calcine 13min;400 DEG C of calcining 100min;340 DEG C of calcining 25min;200 DEG C of calcining 27min;120 DEG C of calcining 36min;Roasting knot
It is cooling in dry gas after beam, obtain activated carbon.Dry gas ingredient and its content are 87%N2And 13%HCl.System of the present invention
Standby activated carbon surface is coarse, and pore structure is abundant, and hole inner wall adheres to a small amount of nano particle, these particles arrangement dispersion, nothing
Sequence.It is largely hydroxyl that activated carbon hole, which also has a large amount of active group, these active groups, and fraction is carboxyl, carbonyl
Deng.Above-mentioned active group plays a key effect in the activity for improving L-phenylalanine lyase, but specific mechanism is still unknown
Really.
Embodiment 2:
Improve the liquid fertilizer of Kiwi berry graft survival rate, it is characterised in that ingredient and its parts by weight include:10.1 parts of auximone,
3.7 parts of 29 parts of zymotic fluid, 0.003 part of sodium molybdate, 1.1 parts of chelated iron, 2.4 parts of citric acid and activated carbon.Above-mentioned liquid fertilizer can be effective
Stimulate L-Phenylalanine Ammonia-Lyase Gene PAL(Phenylalanine ammonia-lyase)Efficient transcription is improving stock
While with the content of scion binding site L-phenylalanine lyase, moreover it is possible to improve L-phenylalanine to a certain extent
The activity of aminonialyase improves grafting success rate to improve the compatibility of stock and scion.For a period of time using liquid fertilizer of the present invention
Afterwards, NR activity improves in scion cell, to NO3 -The absorbability of-N enhances, NH4 +- N conversion metabolism is fast, reduces NH4 +
The accumulation of-N, while scion soluble sugar, proline, soluble protein content and expression can be improved, the increasing of tune ability is oozed in scion
By force, salt tolerance significantly improves.
Auximone ingredient and its parts by weight are 0.6 part of 7.4 parts of heteroauxin, 2.1 parts of zeatin and gibberellin.
The preparation method of zymotic fluid is:By 25 parts of discarded animal bones, 61 parts of feces of livestock and poultry, 26 parts of stalks, 44 portions of useless sugar
Water and 16 parts of durian shells are pulverized and mixed, and 2.6 parts of papains and 2.4 parts of spinach are added after 15 parts of EM bacteria agent anaerobic fermentation 8d are added
Trailing plants protease continues anaerobic fermentation 17d, and 180 parts of water are added, are fully filtered after reaction, the filtrate after concentration is zymotic fluid.This
Not only contain three kinds of nitrogen, phosphorus, potassium a great number of elements in invention liquid fertilizer, also contains abundant weight element, trace element and various
Amino acid, during Kiwi berry grafting can be met, stock and the nutrients needed for the differentiation of scion binding site cell fast breeding
Matter accelerates calli induction and differentiation.
The preparation method of chelated iron is:By FeSO4It is dissolved in the deionized water of 76 times of quality, is uniformly dissolved;By EDTA ~
2Na is dissolved in 27 times of deionized water, is uniformly dissolved;Above two solution is uniformly mixed, 38min is stirred, can must chelate
Iron hydrate;By chelated iron hydrate, 67 DEG C of drying are placed in, chelated iron is obtained.
The preparation method of activated carbon is:Be added in coconut shell powder 3% silicate solid, 0.4% Strong oxdiative magnesium solid and
0.6% manganese acetate roasts under limited oxygen condition, and calcination temperature variation is 110 DEG C of calcining 14min;200 DEG C of calcining 15min;300℃
Calcine 13min;400 DEG C of calcining 100min;340 DEG C of calcining 25min;200 DEG C of calcining 27min;120 DEG C of calcining 36min;Roasting knot
It is cooling in dry gas after beam, obtain activated carbon.Dry gas ingredient and its content are 87%N2And 13%HCl.System of the present invention
Standby activated carbon surface is coarse, and pore structure is abundant, and hole inner wall adheres to a small amount of nano particle, these particles arrangement dispersion, nothing
Sequence.It is largely hydroxyl that activated carbon hole, which also has a large amount of active group, these active groups, and fraction is carboxyl, carbonyl
Deng.Above-mentioned active group plays a key effect in the activity for improving L-phenylalanine lyase, but specific mechanism is still unknown
Really.
Embodiment 3:
The liquid fertilizer of Kiwi berry graft survival rate is improved, ingredient and its parts by weight include:10.5 parts of auximone, zymotic fluid 32
Part, 0.003 part of sodium molybdate, 0.7 part of chelated iron, 2.7 parts of citric acid and 3.4 parts of activated carbon.Above-mentioned liquid fertilizer can effective stimulus L-
Phenylalanine Ammonia-Lyase Gene PAL(Phenylalanine ammonia-lyase)Efficient transcription is improving stock and scion knot
While closing the content of position L-phenylalanine lyase, moreover it is possible to improve L-phenylalanine lyase to a certain extent
Activity improves grafting success rate to improve the compatibility of stock and scion.Using liquid fertilizer of the present invention for a period of time after, scion
NR activity improves in cell, to NO3 -The absorbability of-N enhances, NH4 +- N conversion metabolism is fast, reduces NH4 +- N's
Accumulation, while scion soluble sugar, proline, soluble protein content and expression can be improved, the tune ability of oozing of scion enhances, resistance to
Salt significantly improves.
Auximone ingredient and its parts by weight are 0.5 part of 7.8 parts of heteroauxin, 2.2 parts of zeatin and gibberellin.
The preparation method of zymotic fluid is:By 22 parts of discarded animal bones, 56 parts of feces of livestock and poultry, 23 parts of stalks, 41 portions of useless sugar
Water and 15 parts of durian shells are pulverized and mixed, and 2.4 parts of papains and 2.6 parts of spinach are added after 13 parts of EM bacteria agent anaerobic fermentation 8d are added
Trailing plants protease continues anaerobic fermentation 17d, and 180 parts of water are added, are fully filtered after reaction, the filtrate after concentration is zymotic fluid.This
Not only contain three kinds of nitrogen, phosphorus, potassium a great number of elements in invention liquid fertilizer, also contains abundant weight element, trace element and various
Amino acid, during Kiwi berry grafting can be met, stock and the nutrients needed for the differentiation of scion binding site cell fast breeding
Matter accelerates calli induction and differentiation.
The preparation method of chelated iron is:By FeSO4It is dissolved in the deionized water of 76 times of quality, is uniformly dissolved;By EDTA ~
2Na is dissolved in 27 times of deionized water, is uniformly dissolved;Above two solution is uniformly mixed, 38min is stirred, can must chelate
Iron hydrate;By chelated iron hydrate, 67 DEG C of drying are placed in, chelated iron is obtained.
The preparation method of activated carbon is:Be added in coconut shell powder 4% silicate solid, 0.5% Strong oxdiative magnesium solid and
0.6% manganese acetate roasts under limited oxygen condition, and calcination temperature variation is 110 DEG C of calcining 14min;200 DEG C of calcining 15min;300℃
Calcine 13min;400 DEG C of calcining 100min;340 DEG C of calcining 25min;200 DEG C of calcining 27min;120 DEG C of calcining 36min;Roasting knot
It is cooling in dry gas after beam, obtain activated carbon.Dry gas ingredient and its content are 87%N2And 13%HCl.System of the present invention
Standby activated carbon surface is coarse, and pore structure is abundant, and hole inner wall adheres to a small amount of nano particle, these particles arrangement dispersion, nothing
Sequence.It is largely hydroxyl that activated carbon hole, which also has a large amount of active group, these active groups, and fraction is carboxyl, carbonyl
Deng.Above-mentioned active group plays a key effect in the activity for improving L-phenylalanine lyase, but specific mechanism is still unknown
Really.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (6)
1. improving the liquid fertilizer of Kiwi berry graft survival rate, it is characterised in that ingredient and its parts by weight include:Auximone 8.2 ~
18.6 parts, 20 ~ 40 parts of zymotic fluid, 0.001 ~ 0.004 part of sodium molybdate, 0.4 ~ 1.5 part of chelated iron, 1 ~ 4 part of citric acid and activated carbon 2
~ 4 parts.
2. the liquid fertilizer according to claim 1 for improving Kiwi berry graft survival rate, it is characterised in that:The plant growth
Plain ingredient and its parts by weight are 0.2 ~ 0.6 part of 7 ~ 15 parts of heteroauxin, 1 ~ 3 part of zeatin and gibberellin.
3. the liquid fertilizer according to claim 1 for improving Kiwi berry graft survival rate, it is characterised in that:The zymotic fluid
Preparation method is:By 10 ~ 30 parts of discarded animal bones, 50 ~ 70 parts of feces of livestock and poultry, 20 ~ 30 parts of stalks, 30 ~ 50 parts of useless syrup and
10 ~ 20 parts of durian shells are pulverized and mixed, and 2 ~ 3 parts of papains and 2 ~ 3 are added after 8 ~ 19 parts of 5 ~ 10d of EM bacteria agent anaerobic fermentation are added
Part bromelain, continues 10 ~ 20d of anaerobic fermentation, and 150 ~ 200 parts of water are added, are fully filtered after reaction, the filtrate after concentration is
For zymotic fluid.
4. the liquid fertilizer according to claim 1 for improving Kiwi berry graft survival rate, it is characterised in that:The chelated iron
Preparation method is:By FeSO4It is dissolved in the deionized water of 70 ~ 80 times of quality, is uniformly dissolved;EDTA ~ 2Na is dissolved in 25 ~ 30
In deionized water again, it is uniformly dissolved;Above two solution is uniformly mixed, 30 ~ 45min is stirred, chelated iron hydrate can be obtained;
By chelated iron hydrate, 60 ~ 75 DEG C of drying are placed in, chelated iron is obtained.
5. the liquid fertilizer according to claim 1 for improving Kiwi berry graft survival rate, it is characterised in that:The activated carbon
Preparation method is:2 ~ 4% silicate solids, 0.1 ~ 0.6% Strong oxdiative magnesium solid and 0.2 ~ 0.8% acetic acid are added in coconut shell powder
Manganese roasts under limited oxygen condition, and calcination temperature variation is 100 ~ 120 DEG C of 10 ~ 15min of calcining;200 ~ 220 DEG C of calcinings 10 ~
15min;300 ~ 320 DEG C of 10 ~ 15min of calcining;380 ~ 400 DEG C of 100 ~ 120min of calcining;320 ~ 350 DEG C of 20 ~ 30min of calcining;200
~ 220 DEG C of 20 ~ 30min of calcining;100 ~ 120 DEG C of 30 ~ 40min of calcining;It is cooling in dry gas after roasting, obtain activity
Charcoal.
6. according to the liquid fertilizer of the raising Kiwi berry graft survival rate described in claim 5, it is characterised in that:The dry gas at
Divide and its content is 80 ~ 90%N2With 10 ~ 20%HCl.
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6277419B1 (en) * | 2000-01-31 | 2001-08-21 | Naohiko Sato | Methods of enhancing vitality of plants trees and crops with stevia |
CN102132653A (en) * | 2010-12-21 | 2011-07-27 | 上海博琛生物科技有限公司 | Method for cultivating strong root systems of grafted cucumbers |
CN107098764A (en) * | 2017-06-04 | 2017-08-29 | 贵州村华秋实现代生态农业有限公司 | A kind of red kiwifruit grafting agent |
CN107473820A (en) * | 2017-08-24 | 2017-12-15 | 兰溪市奥而特农业科技有限公司 | Kiwi berry grafting nutrient solution |
-
2018
- 2018-03-01 CN CN201810171532.XA patent/CN108503425A/en not_active Withdrawn
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6277419B1 (en) * | 2000-01-31 | 2001-08-21 | Naohiko Sato | Methods of enhancing vitality of plants trees and crops with stevia |
CN102132653A (en) * | 2010-12-21 | 2011-07-27 | 上海博琛生物科技有限公司 | Method for cultivating strong root systems of grafted cucumbers |
CN107098764A (en) * | 2017-06-04 | 2017-08-29 | 贵州村华秋实现代生态农业有限公司 | A kind of red kiwifruit grafting agent |
CN107473820A (en) * | 2017-08-24 | 2017-12-15 | 兰溪市奥而特农业科技有限公司 | Kiwi berry grafting nutrient solution |
Non-Patent Citations (1)
Title |
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薛智勇等: "《农业固体废物处理与处置》", 30 November 2016, 河南科学技术出版社 * |
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Application publication date: 20180907 |