CN108498635A - To having the ganoderma lucidum Chinese herbal and crude drugs preparations of rehabilitative action after cancer patient chemicotherapy - Google Patents
To having the ganoderma lucidum Chinese herbal and crude drugs preparations of rehabilitative action after cancer patient chemicotherapy Download PDFInfo
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Abstract
The invention belongs to health products preparation fields, and in particular to have the ganoderma lucidum Chinese herbal and crude drugs preparations of rehabilitative action after a kind of chemicotherapy to cancer patient.Preparation method includes the following steps:1)The preparation of ganoderma lucidum growth substrate:It by the dry product of solanum lyratum, gynostemma pentaphylla, white fungus, longan, peanut red coat and Radix Glycyrrhizae, crushes, is dissolved in sterile water and boiling, add glucose and agar powder, stir, be sub-packed in triangular flask, silica gel plug sealing sterilizing;Taking-up is sub-packed in culture dish, is inoculated with after matrix set;2)Inoculation, culture:Each culture dish accesses lucidum strain, and inoculation is placed on light culture in 28 30 DEG C of incubators, waits for that Ganoderma lucidum mycelium is covered with stromal surface, the material of each culture dish is all collected, and crushes, obtains Ganoderma lucidum mycelium Traditional Chinese drug mixture powder;3)By step 2)Lozenge is made in Ganoderma lucidum mycelium Traditional Chinese drug mixture powder obtained.Lozenge obtained by the present invention after cancer patient chemicotherapy to having rehabilitative action.
Description
Technical field
The invention belongs to health products preparation fields, and in particular to have the spirit of rehabilitative action after a kind of chemicotherapy to cancer patient
Sesame Chinese herbal and crude drugs preparations.
Background technology
With the development and progress of medical technology, after treatment, survival period gradually extends cancer patient.But cancer patient one
As will receive chemicotherapy, chemicotherapy generally causes entire patient's discomfort, and dizzy, dizzy, lassitude, weak, appetite subtract
It moves back, Nausea and vomiting, Neuroleptic Leukocytopenia and alopecia etc..The degree of reaction and the size of chemotherapeutics dosage and radiotherapy exposure have
It closes.Because of disease injury, body is constantly repaired again, and cancer patient has to high nutrition.Cancer cell is given birth in histoorgan dilatancy
Long and diffusion, destroy normal structure, and the severe jamming metabolism of body causes patient serious subalimentation occur;
Due to large dosageization, the application of radiotherapy, the nutritional support during treatment is also critically important.Also, after chemicotherapy, at patient body
In extremely feeble state, how to make that patient quickly regains one's vigor and muscle power, energy band disease are lived in more preferably state, is that there is an urgent need for solutions
Certainly the problem of.
Invention content
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to which tool rehabilitation is made after providing a kind of chemicotherapy to cancer patient
Ganoderma lucidum Chinese herbal and crude drugs preparations.Ganoderma lucidum Chinese herbal and crude drugs preparations obtained by the present invention are rich in high protein, high nutrition, low fat, higher-dimension
Raw element, and be easy to digest, extremely meet the nutritional need after cancer patient's chemicotherapy.
To achieve the purpose of the present invention, it adopts the following technical scheme that:
The preparation method of the ganoderma lucidum Chinese herbal and crude drugs preparations of tool rehabilitative action, includes the following steps after a kind of chemicotherapy to cancer patient:
1)The preparation of ganoderma lucidum growth substrate:Count in parts by weight, 10-15 parts of solanum lyratum, 8-12 parts of gynostemma pentaphylla, 10-15 parts of white fungus,
4-6 parts of longan, 2-3 parts of peanut red coat, 5-6 parts of Radix Glycyrrhizae, 20-25 parts of glucose, 5-10 parts of agar powder, 800-850 parts of sterile water;
The dry product of solanum lyratum, gynostemma pentaphylla, white fungus, longan, peanut red coat and Radix Glycyrrhizae is weighed by formula, is crushed, is dissolved in sterile water and boiling,
Glucose and agar powder are added, is stirred, pH value is adjusted to 5.5-6.5, is sub-packed in 500mL triangular flasks, silica gel plug sealing;It adopts
With high-pressure steam sterilizing pan sterilization, 130 DEG C, sterilize 30-40 minutes;Taking-up is sub-packed in the culture dish of diameter 6cm, loading amount
For 20mL, it is inoculated with after matrix set;
2)Inoculation, culture:Each culture dish accesses lucidum strain, inoculum concentration 1-5wt%, and inoculation is placed on 28-30 DEG C of incubator
Middle light culture waits for that Ganoderma lucidum mycelium is covered with stromal surface, and the material of each culture dish is all collected, and crushes, obtains ganoderma lucidum
Mycelia-Traditional Chinese drug mixture powder;
3)By step 2)It after Ganoderma lucidum mycelium obtained-Traditional Chinese drug mixture powder is sterilized, is added in two-dimensional motion mixer, is added
Magnesium stearate, D-sorbite mix 10min;
4)Tabletting:By step 3)Particle after mixing, which is placed in the hopper of rotary tablet machine, carries out tabletting, every in tableting processes
30min survey piece weights are primary, and control every is in 2.45 ~ 2.50g/ pieces.
Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and magnesium stearate is:10:0.01~0.5.
Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and D-sorbite is:10:2~3.
A kind of preparation method as described above is obtained to having the ganoderma lucidum medium-height grass of rehabilitative action after cancer patient chemicotherapy
Medicine preparation.
Solanum lyratum has effects that heat-clearing, dampness removing, wind-dispelling, removing toxic substances, the main effective ingredient of gynostemma pentaphylla are gynosaponins, twist
The blue glucoside of stock(Polysaccharide), water-soluble amino acids, flavonoids, multivitamin, trace element, minerals etc..
The effect of white fungus is sweet in flavor, light, mild-natured, nontoxic, existing tonifying spleen appetizing, and play the role of QI invigorating gut purge, nourishing Yin and moistening lung.
The tolerance that can be enhanced human immunity and enhance tumor patient to Radiotherapy chemotherapy.White fungus is rich in tremella polysaccharides, and white fungus is more
Sugar is Basidiomycetes polysaccharide para-immunity reinforcing agent, plays the role of improving body's immunity and promotes leucocyte.
Peanut red coat can inhibit fibrinous dissolving, increase the content of blood platelet, improve the quality of blood platelet, improve solidifying
The defect of blood factor reinforces the contraction function of capillary, promotes marrow hemopoiesis function.
Hypo acid contained by Radix Glycyrrhizae can block the effect of Induced by Carcinogen tumour growth.
For longan containing abundant glucose, sucrose and protein etc., iron-holder is also relatively high, can improve thermal energy, Replacement Battalion
Promote hemoglobin regeneration while foster, to achieve the effect that enrich blood.
For ganoderma lucidum mycelium to mouse S180 inhibiting rates up to 87.6%, ingredient contains polysaccharide and protein, and antitumor action is aobvious
It writes.
Ganoderma lucidum can be soaked or brews up, but taste is bitter, and common people cannot receive;Although resistance can be enhanced
Power, but effect is but less apparent.Ganoderma lucidum and related Chinese medicine are used in compounding, because Chinese medicine compounding is more complicated engineering,
It is related to monarch combination principle, therefore it is also a problem to prepare a kind of significant Chinese medicine composition of drug effect.
The present invention has the following advantages compared with the prior art:
1)The present invention using solanum lyratum, gynostemma pentaphylla, white fungus, longan, peanut red coat, Radix Glycyrrhizae as the main component of Medium for Ganoderma lucidum,
For cultivating ganoderma lucidum mycelium.On the one hand nutritional ingredient in Chinese medicine can provide necessary nutrient for ganoderma lucidum grown cultures
Deng, on the other hand these nutritional ingredients can be absorbed by ganoderma lucidum, in ganoderma lucidum mycelium growth course, the active constituent hair with ganoderma lucidum
Raw bioconversion, culture obtain the ganoderma lucidum mycelium that a variety of drug effects are integrated in one;
2)Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder obtained by the present invention is rich in high protein, high nutrition, low fat, homovitamin,
And be easy to digest, extremely meet the nutritional need after cancer patient's chemicotherapy, can make patient quickly regain one's vigor and muscle power,
Energy band disease is lived in more preferably state.
Specific implementation mode
Further to disclose rather than the present invention is limited, the present invention is described in further detail below in conjunction with example.
Embodiment 1
The preparation method of the ganoderma lucidum Chinese herbal and crude drugs preparations of tool rehabilitative action, includes the following steps after a kind of chemicotherapy to cancer patient:
1)The preparation of ganoderma lucidum growth substrate:Count in parts by weight, 10 parts of solanum lyratum, 12 parts of gynostemma pentaphylla, 10 parts of white fungus, 6 parts of longan,
2 parts of peanut red coat, 6 parts of Radix Glycyrrhizae, 20 parts of glucose, 10 parts of agar powder, 800 parts of sterile water;Solanum lyratum, strand are weighed by formula
Indigo plant, white fungus, longan, peanut red coat and the dry product of Radix Glycyrrhizae crush, are dissolved in sterile water and boiling, add glucose and agar powder,
Stirring adjusts pH value to 6.0, is sub-packed in 500mL triangular flasks, silica gel plug sealing;Using high-pressure steam sterilizing pan sterilization,
It 130 DEG C, sterilizes 35 minutes;Taking-up is sub-packed in the culture dish of diameter 6cm, and loading amount 20mL is inoculated with after matrix set;
2)Inoculation, culture:Each culture dish accesses lucidum strain, inoculum concentration 2wt%, and inoculation is placed in 29 DEG C of incubators dark
Culture, waits for that Ganoderma lucidum mycelium is covered with stromal surface, the material of each culture dish is all collected, and crushes, obtains Ganoderma lucidum mycelium-
Traditional Chinese drug mixture powder;
3)By step 2)It after Ganoderma lucidum mycelium obtained-Traditional Chinese drug mixture powder is sterilized, is added in two-dimensional motion mixer, is added
Magnesium stearate, D-sorbite mix 10min;
4)Tabletting:By step 3)Particle after mixing, which is placed in the hopper of rotary tablet machine, carries out tabletting, every in tableting processes
30min survey piece weights are primary, and control every is in 2.45 ~ 2.50g/ pieces.
Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and magnesium stearate is:10:0.01.
Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and D-sorbite is:10:2.
Embodiment 2
The preparation method of the ganoderma lucidum Chinese herbal and crude drugs preparations of tool rehabilitative action, includes the following steps after a kind of chemicotherapy to cancer patient:
1)The preparation of ganoderma lucidum growth substrate:Count in parts by weight, 12 parts of solanum lyratum, 10 parts of gynostemma pentaphylla, 12 parts of white fungus, 5 parts of longan,
3 parts of peanut red coat, 5 parts of Radix Glycyrrhizae, 22 parts of glucose, 8 parts of agar powder, 820 parts of sterile water;By formula weigh solanum lyratum, gynostemma pentaphylla,
White fungus, longan, peanut red coat and the dry product of Radix Glycyrrhizae crush, are dissolved in sterile water and boiling, add glucose and agar powder, stir
It mixes, adjusts pH value to 5.5, be sub-packed in 500mL triangular flasks, silica gel plug sealing;Using high-pressure steam sterilizing pan sterilization,
It 130 DEG C, sterilizes 40 minutes;Taking-up is sub-packed in the culture dish of diameter 6cm, and loading amount 20mL is inoculated with after matrix set;
2)Inoculation, culture:Each culture dish accesses lucidum strain, inoculum concentration 3wt%, and inoculation is placed in 30 DEG C of incubators dark
Culture, waits for that Ganoderma lucidum mycelium is covered with stromal surface, the material of each culture dish is all collected, and crushes, obtains Ganoderma lucidum mycelium-
Traditional Chinese drug mixture powder;
3)By step 2)It after Ganoderma lucidum mycelium obtained-Traditional Chinese drug mixture powder is sterilized, is added in two-dimensional motion mixer, is added
Magnesium stearate, D-sorbite mix 10min;
4)Tabletting:By step 3)Particle after mixing, which is placed in the hopper of rotary tablet machine, carries out tabletting, every in tableting processes
30min survey piece weights are primary, and control every is in 2.45 ~ 2.50g/ pieces.
Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and magnesium stearate is:10:0.2.
Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and D-sorbite is:10:2.5.
Embodiment 3
The preparation method of the ganoderma lucidum Chinese herbal and crude drugs preparations of tool rehabilitative action, includes the following steps after a kind of chemicotherapy to cancer patient:
1)The preparation of ganoderma lucidum growth substrate:Count in parts by weight, 15 parts of solanum lyratum, 8 parts of gynostemma pentaphylla, 15 parts of white fungus, 4 parts of longan,
3 parts of peanut red coat, 5 parts of Radix Glycyrrhizae, 25 parts of glucose, 5 parts of agar powder, 850 parts of sterile water;By formula weigh solanum lyratum, gynostemma pentaphylla,
White fungus, longan, peanut red coat and the dry product of Radix Glycyrrhizae crush, are dissolved in sterile water and boiling, add glucose and agar powder, stir
It mixes, adjusts pH value to 6.5, be sub-packed in 500mL triangular flasks, silica gel plug sealing;Using high-pressure steam sterilizing pan sterilization,
It 130 DEG C, sterilizes 40 minutes;Taking-up is sub-packed in the culture dish of diameter 6cm, and loading amount 20mL is inoculated with after matrix set;
2)Inoculation, culture:Each culture dish accesses lucidum strain, inoculum concentration 5wt%, and inoculation is placed in 30 DEG C of incubators dark
Culture, waits for that Ganoderma lucidum mycelium is covered with stromal surface, the material of each culture dish is all collected, and crushes, obtains Ganoderma lucidum mycelium-
Traditional Chinese drug mixture powder;
3)By step 2)It after Ganoderma lucidum mycelium obtained-Traditional Chinese drug mixture powder is sterilized, is added in two-dimensional motion mixer, is added
Magnesium stearate, D-sorbite mix 10min;
4)Tabletting:By step 3)Particle after mixing, which is placed in the hopper of rotary tablet machine, carries out tabletting, every in tableting processes
30min survey piece weights are primary, and control every is in 2.45 ~ 2.50g/ pieces.
Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and magnesium stearate is:10:0.5.
Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and D-sorbite is:10:3.
Polysaccharide and triterpene are the main pharmacodynamics ingredients of ganoderma lucidum, thus polysaccharide and triterpene content number also become and weigh ganoderma lucidum
The important indicator of quality height.
Comparative example 1
The mycelial culture of Common ganoderma lucidum:
Inoculation, culture:Lucidum strain, inoculum concentration 5wt% are accessed in PDA culture medium, inoculation is placed in 30 DEG C of incubators dark
Culture, waits for that Ganoderma lucidum mycelium is covered with stromal surface, takes ganoderma lucidum mycelium, detect its polysaccharide and triterpene content.Meanwhile to embodiment 1-
3 ganoderma lucidum myceliums cultivated also are detected.
1)Measurement of the polysaccharide content
1. the processing of sample:0.3 gram of mycelia powder for taking different embodiment cultures is respectively placed in the volumetric flask of 50ml dryings,
Add water about 40ml, 2h is heated in boiling water bath, and shaked frequently, scale is settled to after being cooled to room temperature, by said extracted
Liquid centrifuges 10min in the centrifuge of 4000r/min, takes supernatant spare.2. formulation polysaccharide=total reducing sugar-reduced sugar of sample
Phenol-sulphate method and DNS methods is respectively adopted in the measurement of total reducing sugar and reduced sugar.
2)The preparation for measuring 1. sample of triterpene content:The hypha powder 0.3g for taking drying is placed in 50ml volumetric flasks and adds
Water is to scale, in ultrasonoscope after ultrasonication 2.5h, centrifuges 10min in the centrifuge of 4000r/min, takes supernatant
It is spare.2. the measurement of sample:The measurement of triterpene uses spectrophotometry.
1 polysaccharide of table and triterpene comparision contents wt/%
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with repair
Decorations should all belong to the covering scope of the present invention.
Claims (4)
1. having the preparation method of the ganoderma lucidum Chinese herbal and crude drugs preparations of rehabilitative action after a kind of chemicotherapy to cancer patient, it is characterised in that:
Include the following steps:
1)The preparation of ganoderma lucidum growth substrate:Count in parts by weight, 10-15 parts of solanum lyratum, 8-12 parts of gynostemma pentaphylla, 10-15 parts of white fungus,
4-6 parts of longan, 2-3 parts of peanut red coat, 5-6 parts of Radix Glycyrrhizae, 20-25 parts of glucose, 5-10 parts of agar powder, 800-850 parts of sterile water;
The dry product of solanum lyratum, gynostemma pentaphylla, white fungus, longan, peanut red coat and Radix Glycyrrhizae is weighed by formula, is crushed, is dissolved in sterile water and boiling,
Glucose and agar powder are added, is stirred, pH value is adjusted to 5.5-6.5, is sub-packed in 500mL triangular flasks, silica gel plug sealing;It adopts
With high-pressure steam sterilizing pan sterilization, 130 DEG C, sterilize 30-40 minutes;Taking-up is sub-packed in the culture dish of diameter 6cm, loading amount
For 20mL, it is inoculated with after matrix set;
2)Inoculation, culture:Each culture dish accesses lucidum strain, inoculum concentration 1-5wt%, and inoculation is placed on 28-30 DEG C of incubator
Middle light culture waits for that Ganoderma lucidum mycelium is covered with stromal surface, and the material of each culture dish is all collected, and crushes, obtains ganoderma lucidum
Mycelia-Traditional Chinese drug mixture powder;
3)By step 2)It after Ganoderma lucidum mycelium obtained-Traditional Chinese drug mixture powder is sterilized, is added in two-dimensional motion mixer, is added
Magnesium stearate, D-sorbite mix 10min;
4)Tabletting:By step 3)Particle after mixing, which is placed in the hopper of rotary tablet machine, carries out tabletting, every in tableting processes
30min survey piece weights are primary, and control every is in 2.45 ~ 2.50g/ pieces.
2. having the preparation of the ganoderma lucidum Chinese herbal and crude drugs preparations of rehabilitative action after the chemicotherapy according to claim 1 to cancer patient
Method, it is characterised in that:Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and magnesium stearate is:10:0.01~
0.5。
3. having the preparation of the ganoderma lucidum Chinese herbal and crude drugs preparations of rehabilitative action after the chemicotherapy according to claim 1 to cancer patient
Method, it is characterised in that:Step 3)The mass ratio of middle Ganoderma lucidum mycelium-Traditional Chinese drug mixture powder and D-sorbite is:10:2~3.
4. a kind of preparation method as described in any one of claims 1-3 is obtained to having rehabilitative action after cancer patient chemicotherapy
Ganoderma lucidum Chinese herbal and crude drugs preparations.
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