CN108498397A - A kind of preparation method of the composite enzyme of skin care whitening - Google Patents
A kind of preparation method of the composite enzyme of skin care whitening Download PDFInfo
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- CN108498397A CN108498397A CN201810377568.3A CN201810377568A CN108498397A CN 108498397 A CN108498397 A CN 108498397A CN 201810377568 A CN201810377568 A CN 201810377568A CN 108498397 A CN108498397 A CN 108498397A
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Classifications
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The present invention provides a kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that includes step in detail below：S1,36 52 parts of Yunnan olive, 22 39 parts of Radix Paeoniae Alba, 11 18 parts of mulberries, 12 17 parts of apple, 38 parts of hawthorn, 16 parts of tomato are cleaned, Yunnan olive stoning, all apparatus use after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；The preparation of S2, probiotics；S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, under the conditions of 34 39 °C, ferment 24 days, with 100 mesh screens, obtain filtrate.The present invention is using pure natural plant ingredients as main function formula, it is compounded by the synergistic between each functional component, synergistic function is shown in terms of whitening, anti-aging, tyrosine can be effectively inhibited and formed, removed free radical, improve dermal photodamage.
Description
Technical field
The invention belongs to technical field of functional food, and in particular to a kind of preparation method of the composite enzyme of skin care whitening.
Background technology
Understanding of the modern medicine to the dark yellow Biological essence of the colour of skin：Mechanism study about skin aging obtain it is very more, successively
There are many theories, but can disclose relatively reasonablely the dark yellow problem of the colour of skin mainly have free radical causes of senescence, non-enzymatic sugar
Base causes of senescence.The colour of skin is dark yellow with free radical causes of senescence：Free radical (Free Radicals) causes of senescence be earliest by
What Denham Harman put forward in nineteen fifty-five.The theory thinks：Free radical has extremely strong oxidability, when in body
When Radical Metabolism is in non-equilibrium state, excessive free radical will cause body injury, and unsaturated fatty acid is oxidized to
Lipid peroxide (lipid peroxidase, LPO), final product malonaldehyde (malonic dialdehyde, MDA) is very
Strong crosslinking agent is crosslinked with large biological molecules such as protein, nucleic acid, forms slightly solubility substance-lipofuscin.People enters person in middle and old age
Later, with the increase at age, the amount of skin lipofuscin substance gradually increases, to keep the dark yellow phenomenon of the colour of skin more tight
Weight.
The colour of skin is dark yellow with non-glycosylation causes of senescence：Non-glycosylation causes of senescence (also known as Maillard reaction senescence
Say), it is the emphasis of Aging mechanism research in recent years as another important causes of senescence of molecular level.It is non-in organism
Enzyme glycosylation refers to the trip in the macromoleculars such as aldehyde radical or ketone group and protein of reducing sugar under conditions of no enzymatic
It is reacted from amino and generates reversible or Irreversible binding object-advanced glycosylation end product (Advanced glycation
Endproducts, AGEs) process.Dyer etc. has found that skin aging is related with non-glycosylation, rich in the skin corium of skin
Containing collagen and elastin laminin, the amino acid in protein molecule is easy aldehyde radical or ketone group with glucose in extracellular fluid
Non-enzymatic glycation occurs, and with advancing age, AGEs progressive increases, and AGEs forms brown color after brown stain occurs
Biologic garbage, be deposited in the skin corium of skin, enable skin lose exquisitely carved sense and yellowing, obscure.In addition, AGEs increases in skin
Add, so that collagen is formed intermolecular cross-linking, not only reduce the elasticity and permeability of skin, make the diffusion of nutriment and waste
Impaired performance, and the solubility for reducing collagen makes it be difficult to, by collagen enzyme hydrolysis, skin elasticity be caused to decline, and wrinkle is not
Easily calm down and constantly deepen, to accelerate the aging course of skin.
Modern medicine solves the problems, such as that the colour of skin is dark yellow, anti-aging is mainly started in terms of above two：1. inhibiting non-enzymatic glycosyl
Change reaction, eliminates harm of the carbonyls to skin；2. removing free radical, avoiding lipid peroxidation, carbonyls is reduced
Generation and the dark yellow formation of the colour of skin.Have effects that the health products type of whitening and anti-aging is various, quality is irregular currently on the market
It is uneven, it can effectively solve the problem that the colour of skin is dark yellow, anti-aging problem, and effective health products are actually rare.
Invention content
In view of this, the present invention provides a kind of preparation method of the composite enzyme of skin care whitening, the present invention is using pure natural
Plant component is compounded, the table in terms of whitening, anti-aging as main function formula by the synergistic between each functional component
Reveal synergistic function, tyrosine can be effectively inhibited and formed, removed free radical, improve dermal photodamage.
The technical scheme is that：A kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that comprising with
Lower specific steps：
S1, by olive 36-52 parts of Yunnan, 22-39 parts of Radix Paeoniae Alba, 11-18 parts of mulberries, 12-17 parts of apple, 3-8 parts of hawthorn, tomato 1-6
Part is cleaned, and Yunnan olive stoning, all apparatus use after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；
The preparation of S2, probiotics：
Prepare mother culture：With weak oxidized acetic acid bacteria（Acetobacter suboxydans）, saccharomyces pastorianus
（Saccharomyces pastorianushansen）, grape juice have 3 kinds of spore Hansenula yeast (Hanseniaspora uvarum)
Probiotics in mass ratio 3:2:1 is combined into mother culture；The culture medium is based on the slurries that S1 steps obtain；
S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, at 34-39 °C
Under the conditions of, it ferments 2-4 days, with 100 mesh screens, obtains filtrate；
S4, the filtrate is sealed, 25-28 °C, low temperature secondary fermentation 3-5 days；Under the conditions of 15-20 °C, then sealing and standing precipitation
5-10 days；
S5, take 20 mesh of the supernatant filtering after step S4 precipitations, sterilization filling at liquid-type Tiny ecosystem ferment.
Further, step S3 includes being added described mother culture 1-4 parts.Particularly, dense in the mother culture
Degree is 2*105cfu/mL。
The present invention also provides a kind of composite enzymes of the skin care whitening prepared by the above method.
The present invention also provides a kind of purposes of composite enzyme of skin care whitening in preparing health food.
In the present invention, Radix Paeoniae Alba (Chlorella vulgaris) is a kind of unicellular fresh water algae, because containing single cell protein
In vain, the nutritional ingredients such as vitamin, nucleic acid and minerals and as animal fodder and health products important source material, so, will be white
Chinese herbaceous peony and Yunnan olive mixed fermentation are a kind of good Utilizing Resource Patterns.
Yunnan olive also known as emblic（PhyllanthusembicaL）, belong to euphorbiaceae phyllanthus emblica category, for fallen leaves Qiao or shrub, fruit
It is real to be rich in vitamin C, 500-l814mg is reached per 100g pulp vitamin contents according to analysis, occupies the hat of general fruit, protein contains
Amount is 2.18010, is far above general fruit；Contents of monosaccharides is 4.96%, disaccharide 0.17%, cellulose 18.76%, starch 11.7%；
Tannin content is up to 17.75%, wherein having glucogallin, gallic acid, benzoaric acid, tanning material stone essence, former myrobalan
Sour also contains the quasi-SOD active material of unique ultra-oxygen anion free radical, therefore determines its sour-puckery flavor, and Hui Tian promotes the production of body fluid after food
Mouthfeel, and with clear scorching sharp larynx, relieving cough and reducing sputum, blood pressure lowering and cholesterol have high nutritive and medicinal value.
Ancients describe that beauty has the sentence of " Liru Chinese herbaceous peony sits such as tree peony ".Peony hold fine, natural beauty, have both color,
Fragrant, rhythm U.S..Chinese herbaceous peony is not single pretty and dazzling, is more valued for Chinese herbaceous peony also with a " medicine " word.The rhizome of Chinese herbaceous peony can be used as medicine, after flower dries
It can be used as jasmine tea to drink, paenoiae alba is even more the top grade of nourishing yin and supplementing blood.Therefore Chinese herbaceous peony also name " flower of female section ".Radix Paeoniae Alba：Bitter, sour, sweet,
It is slightly cold；Return liver, the spleen channel；Can blood-nourishing, for the deficiency of blood or the deficiency of Yin have heat irregular menstruation；Can also nourishing hepatic yin, adjust irritability, Pinggan Yang,
For deficiecny of liver-YIN, liver-qi depression, liver-yang partially high caused headache, dizziness, pain over the hypochondriac region；Cloudy and battalion, hidroschesis can be held back, is used for
Night sweat due to yin deficiency and disharmony between ying and wei exterior deficiency spontaneous perspiration.Can also nourishing blood and liver, dissipate strongly fragrant dissolving stasis, strong the five internal organs, for improving chloasma faciei,
Freckle, chloasma, dark sore caused by endocrine disturbance are treated in pachylosis aging.
Mulberries cold nature, acid sweet in flavor enter the heart, liver and kidney channel, be strengthening by means of tonics, the good fruit of nourishing heart intelligence development, have enrich blood enriching yin,
Liver-kidney tonifying, promote the production of body fluid to quench thirst, ease constipation is dispelled dry and other effects, having a dizzy spell caused by being lost suitable for kidney deficiency and liver and deficiency of blood essence, the soreness of waist
Tinnitus poliosis, insomnia and dreamful sleep, injury thirst, is quenched one's thirst, the diseases such as scrofula, dry constipation of intestines, articular instability.《Diet is occupied with breath
Spectrum》It carries, mulberries energy " nourishing liver kidney, congested liquid, wind-damp dispelling are carried out in vigorous strides, cease empty wind, the clear fire of deficiency type ".
Modern pharmacological research confirms that mulberries can accelerate internal metabolism；Immunity of organisms is significantly increased, is slowed down aging；
Aliphatic acid contained by mulberries has the effects that reduce fat, reduces blood fat, prevents vascular sclerosis；It is compacted that edible mulberries can promote stomach and intestine
It is dynamic, it is anti-to treat constipation；Often feeding mulberries can improve eyesight, the dry and astringent symptom of relieving eye strain；Male eats mulberries more can improve reproduction
The situation of inferior health, regains one's strength of body --- there is this taste medicine of mulberries in the Chinese medicinal formulae of many treatment necrozoospermias.
Mulberries are full of nutrition, according to surveying and determination, wherein containing mulberries oil, carbohydrate, rutin sophorin, anthocyanidin, tannin, malic acid and dimension
The ingredients such as raw element B1, vitamin B2, vitamin C and carrotene.Current research data shows, rutin sophorin, flower contained in mulberries
The ingredients such as green element have the effect of preventing tumour cell diffusion, cancer is avoided to occur.Contain multivitamin in mulberries, also contains rich
Rich phosphorus and iron, can kidney-nourishing enrich blood, make one to have rosy cheeks, hair it is pitch-black beautiful.
The present invention is Promethean by compound in probiotics between Yunnan olive, Radix Paeoniae Alba, mulberries, apple, hawthorn, tomato components
At the synergistic compounding of fermentation process in mother culture, synergistic function is shown in terms of whitening, anti-aging, it can be effective
Ground inhibits tyrosine to be formed, removes free radical, improves dermal photodamage, can significantly enhance whitening, slow down aging.The present invention
Using micro-ecology fermentation technology, probiotics are combined with fresh fruit vegetables, algae three, manufacture " composite enzyme " is mended
Fill agent, i.e. Tiny ecosystem edible ferment.
Specific implementation mode
Technical scheme of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
It is only a part of the embodiment of the present invention to apply example, instead of all the embodiments.
Embodiment 1
A kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that include step in detail below：
S1,45 parts of Yunnan olive, 32 parts of Radix Paeoniae Alba, 15 parts of mulberries, 14 parts of apple, 5 parts of hawthorn, 4 parts of tomato are cleaned, Yunnan olive is gone
Core, all apparatus use after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；
The preparation of S2, probiotics：
Prepare mother culture：With weak oxidized acetic acid bacteria（Acetobacter suboxydans）, saccharomyces pastorianus
（Saccharomyces pastorianushansen）, grape juice have 3 kinds of spore Hansenula yeast (Hanseniaspora uvarum)
Probiotics in mass ratio 3:2:1 is combined into mother culture；The culture medium is based on the slurries that S1 steps obtain；
S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, in 36 °C of items
Under part, ferments 3 days, with 100 mesh screens, obtain filtrate；
S4, the filtrate is sealed, 26 °C, low temperature secondary fermentation 3-5 days；Under the conditions of 18 °C, then sealing and standing precipitates 7 days；
S5, take 20 mesh of the supernatant filtering after step S4 precipitations, sterilization filling at liquid-type Tiny ecosystem ferment.
Further, step S3 includes that 2.2 parts of the mother culture is added.Particularly, dense in the mother culture
Degree is 2*105cfu/mL。
The present invention also provides a kind of composite enzymes of the skin care whitening prepared by the above method.
The present invention also provides a kind of purposes of composite enzyme of skin care whitening in preparing health food.
In the present invention, Radix Paeoniae Alba (Chlorella vulgaris) is a kind of unicellular fresh water algae, because containing single cell protein
In vain, the nutritional ingredients such as vitamin, nucleic acid and minerals and as animal fodder and health products important source material, so, will be white
Chinese herbaceous peony and Yunnan olive mixed fermentation are a kind of good Utilizing Resource Patterns.
The present invention is Promethean by compound in probiotics between Yunnan olive, Radix Paeoniae Alba, mulberries, apple, hawthorn, tomato components
At the synergistic compounding of fermentation process in mother culture, synergistic function is shown in terms of whitening, anti-aging, it can be effective
Ground inhibits tyrosine to be formed, removes free radical, improves dermal photodamage, can significantly enhance whitening, slow down aging.The present invention
Using micro-ecology fermentation technology, probiotics are combined with fresh fruit vegetables, algae three, manufacture " composite enzyme " is mended
Fill agent, i.e. Tiny ecosystem edible ferment.
Embodiment 2
A kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that include step in detail below：
S1,36 parts of Yunnan olive, 22 parts of Radix Paeoniae Alba, 11 parts of mulberries, 12 parts of apple, 3 parts of hawthorn, 1 part of tomato are cleaned, Yunnan olive is gone
Core, all apparatus use after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；
The preparation of S2, probiotics：
Prepare mother culture：With weak oxidized acetic acid bacteria（Acetobacter suboxydans）, saccharomyces pastorianus
（Saccharomyces pastorianushansen）, grape juice have 3 kinds of spore Hansenula yeast (Hanseniaspora uvarum)
Probiotics in mass ratio 3:2:1 is combined into mother culture；The culture medium is based on the slurries that S1 steps obtain；
S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, in 34 °C of items
Under part, ferments 2 days, with 100 mesh screens, obtain filtrate；
S4, the filtrate is sealed, 25 °C, low temperature secondary fermentation 3 days；Under the conditions of 15 °C, then sealing and standing precipitates 5 days；
S5, take 20 mesh of the supernatant filtering after step S4 precipitations, sterilization filling at liquid-type Tiny ecosystem ferment.
Further, step S3 includes that 1 part of the mother culture is added.Particularly, the concentration in the mother culture
For 2*105cfu/mL。
The present invention also provides a kind of composite enzymes of the skin care whitening prepared by the above method.
The present invention also provides a kind of purposes of composite enzyme of skin care whitening in preparing health food.
In the present invention, Radix Paeoniae Alba (Chlorella vulgaris) is a kind of unicellular fresh water algae, because containing single cell protein
In vain, the nutritional ingredients such as vitamin, nucleic acid and minerals and as animal fodder and health products important source material, so, will be white
Chinese herbaceous peony and Yunnan olive mixed fermentation are a kind of good Utilizing Resource Patterns.
Embodiment 3
A kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that include step in detail below：
S1,52 parts of Yunnan olive, 39 parts of Radix Paeoniae Alba, 18 parts of mulberries, 17 parts of apple, 8 parts of hawthorn, 6 parts of tomato are cleaned, Yunnan olive is gone
Core, all apparatus use after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；
The preparation of S2, probiotics：
Prepare mother culture：With weak oxidized acetic acid bacteria（Acetobacter suboxydans）, saccharomyces pastorianus
（Saccharomyces pastorianushansen）, grape juice have 3 kinds of spore Hansenula yeast (Hanseniaspora uvarum)
Probiotics in mass ratio 3:2:1 is combined into mother culture；The culture medium is based on the slurries that S1 steps obtain；
S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, in 39 °C of items
Under part, ferments 4 days, with 100 mesh screens, obtain filtrate；
S4, the filtrate is sealed, 28 °C, low temperature secondary fermentation 5 days；Under the conditions of 20 °C, then sealing and standing precipitates 10 days；
S5, take 20 mesh of the supernatant filtering after step S4 precipitations, sterilization filling at liquid-type Tiny ecosystem ferment.
Further, step S3 includes that 4 parts of the mother culture is added.Particularly, the concentration in the mother culture
For 2*105cfu/mL。
The present invention also provides a kind of composite enzymes of the skin care whitening prepared by the above method.
The present invention also provides a kind of purposes of composite enzyme of skin care whitening in preparing health food.
Embodiment 4
A kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that include step in detail below：
S1,48 parts of Yunnan olive, 27 parts of Radix Paeoniae Alba, 12 parts of mulberries, 14 parts of apple, 7 parts of hawthorn, 3 parts of tomato are cleaned, Yunnan olive is gone
Core, all apparatus use after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；
The preparation of S2, probiotics：
Prepare mother culture：With weak oxidized acetic acid bacteria（Acetobacter suboxydans）, saccharomyces pastorianus
（Saccharomyces pastorianushansen）, grape juice have 3 kinds of spore Hansenula yeast (Hanseniaspora uvarum)
Probiotics in mass ratio 3:2:1 is combined into mother culture；The culture medium is based on the slurries that S1 steps obtain；
S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, in 35 °C of items
Under part, ferments 3 days, with 100 mesh screens, obtain filtrate；
S4, the filtrate is sealed, 27 °C, low temperature secondary fermentation 3-5 days；Under the conditions of 18 °C, then sealing and standing precipitates 8 days；
S5, take 20 mesh of the supernatant filtering after step S4 precipitations, sterilization filling at liquid-type Tiny ecosystem ferment.
Further, step S3 includes that 3.4 parts of the mother culture is added.Particularly, dense in the mother culture
Degree is 2*105cfu/mL。
The present invention also provides a kind of composite enzymes of the skin care whitening prepared by the above method.
The present invention also provides a kind of purposes of composite enzyme of skin care whitening in preparing health food.
Embodiment 5
A kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that include step in detail below：
S1,41 parts of Yunnan olive, 24 parts of Radix Paeoniae Alba, 13 parts of mulberries, 16 parts of apple, 4 parts of hawthorn, 2 parts of tomato are cleaned, all apparatus
It is used after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；
The preparation of S2, probiotics：
Prepare mother culture：With weak oxidized acetic acid bacteria（Acetobacter suboxydans）, saccharomyces pastorianus
（Saccharomyces pastorianushansen）, grape juice have 3 kinds of spore Hansenula yeast (Hanseniaspora uvarum)
Probiotics in mass ratio 3:2:1 is combined into mother culture；The culture medium is based on the slurries that S1 steps obtain；
S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, in 35 °C of items
Under part, ferments 3 days, with 100 mesh screens, obtain filtrate；
S4, the filtrate is sealed, 27 °C, low temperature secondary fermentation 3-5 days；Under the conditions of 18 °C, then sealing and standing precipitates 8 days；
S5, take 20 mesh of the supernatant filtering after step S4 precipitations, sterilization filling at liquid-type Tiny ecosystem ferment.
Further, step S3 includes that 3.4 parts of the mother culture is added.Particularly, dense in the mother culture
Degree is 2*105cfu/mL。
The present invention also provides a kind of composite enzymes of the skin care whitening prepared by the above method.
The present invention also provides a kind of purposes of composite enzyme of skin care whitening in preparing health food.
Comparative example 1
A kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that include step in detail below：
S1,32 parts of Radix Paeoniae Alba, 15 parts of mulberries, 14 parts of apple, 5 parts of hawthorn, 4 parts of tomato are cleaned, Yunnan olive stoning, all apparatus are wanted
It is used after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；
The preparation of S2, probiotics：
Prepare mother culture：With weak oxidized acetic acid bacteria（Acetobacter suboxydans）, saccharomyces pastorianus
（Saccharomyces pastorianushansen）, grape juice have 3 kinds of spore Hansenula yeast (Hanseniaspora uvarum)
Probiotics in mass ratio 3:2:1 is combined into mother culture；The culture medium is based on the slurries that S1 steps obtain；
S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, in 36 °C of items
Under part, ferments 3 days, with 100 mesh screens, obtain filtrate；
S4, the filtrate is sealed, 26 °C, low temperature secondary fermentation 3-5 days；Under the conditions of 18 °C, then sealing and standing precipitates 7 days；
S5, take 20 mesh of the supernatant filtering after step S4 precipitations, sterilization filling at liquid-type Tiny ecosystem ferment.
Further, step S3 includes that 2.2 parts of the mother culture is added.Particularly, dense in the mother culture
Degree is 2*105cfu/mL。
The present invention also provides a kind of composite enzymes of the skin care whitening prepared by the above method.
The present invention also provides a kind of purposes of composite enzyme of skin care whitening in preparing health food.
Comparative example 2
A kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that include step in detail below：
S1,45 parts of Yunnan olive, 15 parts of mulberries, 14 parts of apple, 5 parts of hawthorn, 4 parts of tomato are cleaned, the stoning of Yunnan olive, all apparatus
It is used after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；
The preparation of S2, probiotics：
Prepare mother culture：With weak oxidized acetic acid bacteria（Acetobacter suboxydans）, saccharomyces pastorianus
（Saccharomyces pastorianushansen）, grape juice have 3 kinds of spore Hansenula yeast (Hanseniaspora uvarum)
Probiotics in mass ratio 3:2:1 is combined into mother culture；The culture medium is based on the slurries that S1 steps obtain；
S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, in 36 °C of items
Under part, ferments 3 days, with 100 mesh screens, obtain filtrate；
S4, the filtrate is sealed, 26 °C, low temperature secondary fermentation 3-5 days；Under the conditions of 18 °C, then sealing and standing precipitates 7 days；
S5, take 20 mesh of the supernatant filtering after step S4 precipitations, sterilization filling at liquid-type Tiny ecosystem ferment.
Further, step S3 includes that 2.2 parts of the mother culture is added.Particularly, dense in the mother culture
Degree is 2*105cfu/mL。
The present invention also provides a kind of composite enzymes of the skin care whitening prepared by the above method.
The present invention also provides a kind of purposes of composite enzyme of skin care whitening in preparing health food.
Whitening effect test experiments
1. the anti-oxidant experiment of mouse lipid
Laboratory sample and method
Experimental animal：Healthy 12 monthly age Kunming mouse 60, female, weight 48-60g.It will be made by embodiment 1
Composite enzyme each component mixing, be prepared into after concentrate drying it is powdered, as test sample.
Experiment packet：
Experiment mice is randomly divided into 6 groups by MDA levels in blood, every group of 10 animals, respectively tested material low dose group
（Embodiment 1 prepare product 50mg/kg), tested material middle dose group（1 sample 100mg/kg of embodiment), the high agent of tested material
Amount group（1 sample 150mg/kg of embodiment), positive controls（Embodiment 2 in Chinese patent application 201110415844.9
The product of offer, the i.e. composition of female whitening skin-nourishing, dosage 100mg/kg) and blank control group.
Experimental method：
Three experimental groups, 2 gained pulvis of positive controls difference Example, the dilution of 2 sample warm water of document embodiment
150 times, blank control group gives equivalent warm water, carries out gastric infusion according to grouping, is carried out continuously 30 days.
Last is given after each group handles 30min accordingly, takes animal subject intraocular corner of the eyes venous blood, measures its whole blood trough respectively
The sweet peptide peroxidase of Guang（GSH-px) activity, viable red blood cells superoxide dismutase（SOD) in activity and mouse blood
Lipid peroxide malonaldehyde（MDA) content.
Data statistics：Data carry out variance analysis using 16.0 for windows softwares of SPSS.
Test result
Serum SOD-px determinations of activity：Using RNA isolation kit（Bioengineering Research Institute's purchase is built up in Nanjing）, it is shown in Table 1：
1 given the test agent of table is on the active influences of mouse GSH-px
Note：P1 is significance analysis of each group compared with blank control group as a result, P2 is tested material dosage group and the positive in table
The significance analysis result of control group.
Viable red blood cells SOD determinations of activity：
Using RNA isolation kit（Bioengineering Research Institute's purchase is built up in Nanjing）It is shown in Table 2：
2 given the test agent of table is on the active influences of mouse red blood cell SOD
Note：P1 is significance analysis of each group compared with blank control group as a result, P2 is tested material dosage group and the positive in table
The significance analysis result of control group.
MDA assays in mouse blood：Using RNA isolation kit（Bioengineering Research Institute's purchase is built up in Nanjing）, it is shown in Table
3：
Influence of 3 given the test agent of table to MDA contents in mouse blood
Note：P1 is significance analysis of each group compared with blank control group as a result, P2 is tested material dosage group and the positive in table
The significance analysis result of control group.
Equally, to remaining embodiment and the embodiment of the present invention, remaining proportioning carries out identical experiment, as a result with embodiment 1
Effect is similar.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiment being appreciated that.It is noted that the technical characteristic not being described in detail in the present invention, can pass through this
Field any prior art is realized.
Claims (4)
1. a kind of preparation method of the composite enzyme of skin care whitening, which is characterized in that include step in detail below：
S1, by olive 36-52 parts of Yunnan, 22-39 parts of Radix Paeoniae Alba, 11-18 parts of mulberries, 12-17 parts of apple, 3-8 parts of hawthorn, tomato 1-6
Part is cleaned, and Yunnan olive stoning, all apparatus use after ensuring disinfection；Drying drains surface moisture content, is broken into slurries；
The preparation of S2, probiotics：
Prepare mother culture：There are 3 kinds of probiotics of spore Hansenula yeast by quality with weak oxidized acetic acid bacteria, saccharomyces pastorianus, grape juice
Than 3:2:1 is combined into mother culture；The culture medium is based on the slurries that S1 steps obtain；
S3, by mother culture obtained by the step S1 culture mediums obtained and S2, be put into the fermentation tank, seal, at 34-39 °C
Under the conditions of, it ferments 2-4 days, with 100 mesh screens, obtains filtrate；
S4, the filtrate is sealed, 25-28 °C, low temperature secondary fermentation 3-5 days；Under the conditions of 15-20 °C, then sealing and standing precipitation
5-10 days；
S5, take 20 mesh of the supernatant filtering after step S4 precipitations, sterilization filling at liquid-type Tiny ecosystem ferment.
2. the preparation method of the composite enzyme of skin care whitening according to claim 1, which is characterized in that step S3 includes
It is added described mother culture 1-4 parts.
3. the ferment that method is prepared according to claim 1.
4. purposes of the ferment described in claim 3 in preparing health food.
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