CN108497489A - A kind of composition and its preparation method and application improving immunity - Google Patents
A kind of composition and its preparation method and application improving immunity Download PDFInfo
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- CN108497489A CN108497489A CN201710103722.3A CN201710103722A CN108497489A CN 108497489 A CN108497489 A CN 108497489A CN 201710103722 A CN201710103722 A CN 201710103722A CN 108497489 A CN108497489 A CN 108497489A
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- cordyceps militaris
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/734—Alginic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8967—Lilium, e.g. tiger lily or Easter lily
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of food compositions and diet supplementary for improving immunity.The present invention is using integration of drinking and medicinal herbs raw-food material ginseng, Cordyceps militaris, lily, dried orange peel, trehalose, several components compatibility collocation become simultaneous tonifying lung the moon and kidney yang, it reinforces the spleen to benefit the lung, it promotes the production of body fluid to quench thirst, tranquilize the mind and promote the intelligence, keep cell activity has the food compositions for improving manpower immunity, each component has apparent mutual synergistic effect, has the effect for the immunity that increases significantly.The present invention's there are the food compositions for improving immunity to have apparent promotion rehabilitation efficacy to inferior health and the weak constitution crowd of illness (especially chemotherapy of tumors crowd, leukaemia crowd) by improving the immunity of the human body, and good mouthfeel, it is a kind of health product preferably improved the immunity of the human body with unique fresh flavor.
Description
Technical field
The invention belongs to technical field of food healthcare, and in particular to a kind of composition and preparation method thereof improving immunity
And application.
Background technology
The very fast nervous rhythm of life of modern society's height, unprecedented pressure is caused to the mankind.In nervous work
Make under pressure, excessive mental manual labor, the long-term high-pressure of people's spirit, the body of people is often chronically at stress shape
State causes body immunity to reduce, and further from developing into sub-health state.Immunologic hypofunction, immunologic function disorder etc.
Immune function failure is often that the weight such as cardiovascular and cerebrovascular disease, diabetes, hypertension, hyperlipidemia, fatty liver, cancer occurs for the mankind
The refractory basic reason of the omen of disease and all kinds of diseases and ailments greatly, cannot ignore.Immunity is human body itself epidemic preventing mechanism, is human body
Identify and eliminate external intrusion foreign matter (virus, bacterium etc.) and processing aging, death, damage, the own cells of denaturation and knowledge
Other and processing vivo mutations cell and virus infected cell ability.Therefore, it is prevention inferior health to improve manpower autoimmunity
It is basic.There are many products for improving immunity on the market at present, but the existing product for improving immunity is mostly Chinese medicine, is contained
There is certain side effect, much easily cause user's dry, conditioning body should not be taken for a long time.Searching is more preferably suitable for
Immunity is improved, the daily health caring consumable products of sub-health state are improved, is still that immunity promotes class health food development neck
The vital task in domain.The present inventor invents a kind of food compositions by studying for a long period of time with a large number of experiments, can effectively improve people
Power immunity also has apparent facilitation effect to the recovery of human body diseases.
Invention content
It is mostly Chinese medicine to solve the existing product for improving immunity, containing certain side effect, conditioning body should not be taken for a long time
The problem of body, the present invention are guidance with modern medical theory on Research foundation for many years, will be by the material combination system of integration of drinking and medicinal herbs
Standby enhances cell activity, effectively improves immunologic hypofunction, sub-health population people's immunity, and good mouthfeel has only
The food compositions of special fresh flavor can effectively improve and adjust immune function of human body, so that human body is kept fit state, can help
Sub-health population restores normal human's metabolic balance, and patient groups can be helped faster to return rehabilitation, and without any side effects, fits
Close long-term consumption conditioning health.
An object of the present invention is to provide with the food compositions for improving immunity, and of the invention has raising
The food compositions of immunity are made of the active mixture A and cell activity reinforcing agent B of raising immunity.
In the food compositions of the present invention, the active mixture A of immunity is improved by respectively from ginseng, Cordyceps militaris, dried orange peel
The extract composition obtained with the extraction of lily raw material, cell activity reinforcing agent B are to be derived from trehalose raw material.
Ginseng：Ginseng possesses the good reputation of " King of Herbs " since ancient times, is more described as by oriental medicine circle, and " life is mended in enriching yin, is helped
Just consolidating " superfine product.Mild-natured, sweet in flavor, slight bitter, tepor.Returns spleen, lung channel.Effect：It reinforces vital energy, veins takes off admittedly reinforces the spleen to benefit the lung,
It promotes the production of body fluid to quench thirst, tranquilize the mind and promote the intelligence.For the new resource food of state approval.
Cordyceps militaris：It is called northern Chinese caterpillar Fungus, Cordceps militaris, tonifying lung the moon, kidney-replenishing, the effect of Cordyceps militaris is to control kidney deficiency, impotence
Seminal emission, soreness of waist and knee joint, eak after being ill, Chinese medicine thinks that it serves and strengthens the body resistance to consolidate the constitution, to chronic senile bronchitis, the lung originality heart
Popular name for has significant curative effect, can improve liver detoxification ability, plays liver protection effect, improves that body is antiviral and capability of resistance to radiation.Chinese medicine
Think, cordyceps sinensis enters lung kidney two warp, can tonifying lung the moon and kidney-replenishing.For the new resource food of state approval.
Dried orange peel：The pericarp of rutaceae orange drying and ripening, bitter is pungent, warm-natured, rule lung, the spleen channel.With regulating qi-flowing for strengthening spleen,
It the effect of sticky sputum dampness elimination, is clinically widely used.Integration of drinking and medicinal herbs.
Lily has very high medical value.Removing toxic substances, reason spleen stomach invigorating, dampness removing disperse accumulation, antitoxic heart-soothing and sedative, the work(such as promote blood circulation
Effect.The diseases such as overstrain cough, hemoptysis, dysphoria palpitates with fear are cured mainly, have special effect to curing lung network disease and health-care antisenile.According to pharmacological research
Show that dried lily bulb plays the role of increasing leukocyte, therefore kinds cancer is all had a better effect.Integration of drinking and medicinal herbs.
Trehalose：Trehalose is a kind of safe and reliable natural carbohydrate, and scientific circles are known as the good reputation of " the sugar of life ".Sea
Algae sugar has organism magical protective effect, is because trehalose is in evils such as high temperature, high and cold, hyperosmosis and dry dehydrations
Unique protective film can be formed in cell surface under bad environmental condition, is effectively protected protein molecule invariance inactivation, to
Sustain life the life process and biological characteristic of body.This unique functional characteristic so that trehalose is in addition to that can serve not only as
Natural edible sweet taste sugar, also as the excellent activity protective agent of pharmaceutical grade protein, enzyme, vaccine and other biological product other than, be
The important component for keeping cell activity, more can be used as prevents food deterioration, keeps food fresh flavor, promotes the only of food quality
Special food ingredient.
In the food compositions of the present invention, the parts by weight of raw material are 3~15 parts by weight of ginseng, 1~12 parts by weight of Cordyceps militaris,
10~45 parts by weight of lily, 25~75 parts by weight of dried orange peel, 10~40 parts by weight of trehalose；
Preferably, the parts by weight of raw material are 5~13 parts by weight of ginseng, 3~10 parts by weight of Cordyceps militaris, 15~40 weight of lily
Part, 30~60 parts by weight of dried orange peel, 12~35 parts by weight of trehalose；
The parts by weight of further preferred raw material are 7~11 parts by weight of ginseng, 5~7 parts by weight of Cordyceps militaris, lily 20~30
Parts by weight, 35~50 parts by weight of dried orange peel, 15~30 parts by weight of trehalose；
7~9 parts by weight of weight ratio ginseng of best each raw material, 4~7 parts by weight of Cordyceps militaris, 25~30 parts by weight of lily,
35~40 parts by weight of dried orange peel, 20~25 parts by weight of trehalose.
The present invention also provides a kind of preparation methods of above-mentioned food compositions, include the following steps：
(1) the active mixture A for improving immunity is prepared：
Step A：Selected ginseng, Cordyceps militaris, lily, dried orange peel raw material；
Step B：It weighs；Ratio weighs selected ginseng, Cordyceps militaris, lily, dried orange peel raw material by weight
Step C：Rinse raw material：Clear water is rinsed well；
Step D：It crushes；
Step E：Alcoholic extraction and concentration：By the parts by weight ginseng of weighing, Cordyceps militaris, lily, dried orange peel raw material respectively at wine
Concentration respectively obtains ginseng, Cordyceps militaris, lily, dried orange peel alcohol crude extract respectively after being extracted in essence；
Step F：The ginseng of step 5, Cordyceps militaris, lily, dried orange peel alcohol crude extract are added in boiling water after extracting and are concentrated
Obtain ginseng, Cordyceps militaris, lily, dried orange peel alcohol medicinal extract；
Step G：Filtering；
Step H：Sterilizing, mix active mixture A is saved backup；
(2) it takes by weight and prepares active reinforcing agent B；
(3) A is added in B；
(4) auxiliary material is added and finished product is made
(2) it takes by weight and prepares active reinforcing agent B；
(3) active mixture A is added in active reinforcing agent B；
(4) auxiliary material is added, edible composition finished product is made.
Wherein, the crushing of step D is one kind in grinding, ball milling shearing or air-flow crushing.
Concentration described in step E and F is selected from vacuum-concentrcted, freeze concentration, film concentration and absorption resin separation concentration,
Tubular membrane be concentrated by evaporation in one kind, the drying described in step H is selected from low temperature and spontaneously dries and vacuum drying, fluidized drying, spray
One kind of mist drying, microwave drying.
In an embodiment of the present invention, the crushing of step D is grinding.
The concentration of step E and step F be selected from vacuum decompression evaporations and concentration, freeze concentration, membrane filtration concentrate (reverse osmosis) and
Adsorb one kind in resin separation concentration, tubular membrane evaporation and concentration.
In one embodiment of preparation method of food compositions of the present invention, the concentration of step E and F are vacuum-concentrcted.
In one embodiment of preparation method of food compositions of the present invention, the alcoholic extraction of step E is 70-85 degree edible wines
Essence extraction, alcoholic extraction time are 1~4 hour；The extracting in boiling water time of step F is 10-50 minutes；Concentration is that vacuum decompression is dense
Contracting, is filtered into -120 mesh of 50 mesh sieve refined filtration, and drying temperature is 40~90 DEG C.
Preferably, in the preparation method with the composition for improving immunity of the invention, alcoholic extraction 70-85
Edible alcohol extraction is spent, the alcoholic extraction time is 2~3 hours, and the extracting in boiling water time is 20-50 minutes, is concentrated as vacuum decompression
Concentration, is filtered into -100 mesh of 80 mesh sieve refined filtration, and drying temperature is 60~90 DEG C.
The drying of step H is selected from dry natural low temperature, freeze-drying, drying or vacuum drying.
In an embodiment of the present invention, the drying of step H spontaneously dries for low temperature.
In one embodiment of the invention, the drying temperature of step H is 50 DEG C.
In one embodiment of the invention, the drying temperature of step H is 80 DEG C.
In one embodiment of the invention, the drying temperature of step H is 65 DEG C.
Step H's is mixed into manual mixing or is stirred with mixing machine, and incorporation time is 0.5 to 5 hour.
Being mixed into for step H is stirred with mixing machine in one embodiment of the invention.
The third object of the present invention is to provide a kind of application of the food compositions of the raising immunity of the present invention, is to make
Application in the standby health food for improving hypoimmunity and sub-health population immunity.
The fourth object of the present invention is to provide a kind of application of the food compositions of the raising immunity of the present invention, is to make
Standby conditioning disappears
Change the application in the health food of functional disturbance symptom.
The fifth object of the present invention is to provide a kind of application of the food compositions of the raising immunity of the present invention, is to make
Application in the health food of standby conditioning Respiratory ft tive resistance diagonosis of disorder.
The present invention have improve immunity composition application in, health food include oral solution, capsule and
Tablet, the functional food include beverage, biscuit.
In one embodiment of the present invention, the application for improving the food compositions of immunity is oral solution.
In one embodiment of the present invention, the application for improving the food compositions of immunity is capsule.
In one embodiment of the present invention, the application for improving the food compositions of immunity is tablet.
In one embodiment of the present invention, the application for improving the food compositions of immunity is granule.
In one embodiment of the present invention, the application for improving the food compositions of immunity is biscuit.
Beneficial effects of the present invention are：The present invention is using integration of drinking and medicinal herbs raw-food material ginseng, Cordyceps militaris, lily, dried orange peel, sea
Algae sugar, several components compatibility collocation become simultaneous tonifying lung the moon and kidney yang maintains human body yin-yang balancing, reinforce the spleen to benefit the lung, promote the production of body fluid to quench thirst, pacify
Refreshing intelligence development is conducive to the food compositions with raising manpower immunity for keeping various enzymes and cytokine activity, through numerous
Crowd on probation proves that these types of active material not restriction or checking relation in five elements has apparent synergistic function, has the immunity that increases significantly
Effect.The present invention's has the food compositions for improving immunity weak to inferior health and illness by improving the immunity of the human body
Constitution crowd (especially chemotherapy of tumors crowd, leukaemia crowd) has apparent promotion rehabilitation efficacy, and good mouthfeel, has
Unique fresh flavor, be a kind of health product preferably improved the immunity of the human body.
Specific implementation mode
Following instance illustrates each aspect of the present invention content.They are only to illustrate, and not delimit the scope of the invention.
Embodiment 1. is a kind of to have the granule and preparation method thereof for improving immunity
1. weighing raw material (gram) by following weight ratios：
Ginseng 9, Cordyceps militaris 6, lily 28, dried orange peel 42；
2. after all raw materials are cleaned and crush, being placed in soak extraction 2.5 hours in 85 degree of edible alcohols；Vacuum-concentrcted
Recycle ethyl alcohol, respectively obtain ginseng, Cordyceps militaris, lily, dried orange peel alcoholic extract；
3. the ginseng that step 2 is extracted, Cordyceps militaris, lily, dried orange peel alcoholic extract be added boiling water in extract 30 minutes,
Vacuum-concentrcted obtains the concentration alcohol medicinal extract of ginseng, Cordyceps militaris, lily, dried orange peel, and 100 mesh refined filtration of concentrated extract is removed slag；
4. alcohol medicinal extract low temperature drying will be concentrated obtained by step 3, drying temperature is 70 degree, and trehalose is added in dry postcooling
15, obtain the concentration alcohol medicinal extract and trehalose mixture of ginseng, Cordyceps militaris, lily, dried orange peel.
5. lactose auxiliary material, which is added, is modulated into particle 100, sterilizing is distributed into bag, every bag of 9g.
Embodiment 2. is a kind of to have the oral solution and preparation method thereof for improving immunity
1. weighing raw material (gram) by following weight ratios：
Ginseng 11, Cordyceps militaris 7, lily 26, dried orange peel 45；
2. after all raw materials are cleaned and crush, being placed in 75 degree of edible alcohols and extracting 3 hours；Vacuum-concentrcted recycles second
Alcohol obtains the alcoholic extract of ginseng, Cordyceps militaris, lily, dried orange peel respectively.
3. the ginseng that step 2 is extracted, Cordyceps militaris, lily, dried orange peel alcoholic extract be added boiling water in extract 25 minutes,
Vacuum-concentrcted obtains the concentration alcohol medicinal extract of ginseng, Cordyceps militaris, lily, dried orange peel, and 80 mesh refined filtration of concentrated extract is removed slag.
4. by step 3 gained ginseng, the concentration alcohol medicinal extract low temperature drying of Cordyceps militaris, lily, dried orange peel, drying temperature 80
Trehalose 18 is added in degree, dry postcooling, obtains ginseng, Cordyceps militaris, lily, the concentration alcohol medicinal extract of dried orange peel and trehalose mixing
Object.
5. gained ginseng, Cordyceps militaris, lily, the concentration alcohol medicinal extract of dried orange peel and trehalose mixture are dissolved in medicinal extract weight 5-
It in a concentration of ethanol water according to weight meter 95% of 10 times of weight, is stirred, sterilizes, every bottle of 10ml quantitative separating enters
Bottle, obtains oral liquid formulations.
Embodiment 3.It is a kind of that there is the nutrient troche and preparation method thereof for improving immunity
1. weighing raw material (gram) by following weight ratios：
Ginseng 10, Cordyceps militaris 7, lily 24, dried orange peel 48；
2. after all raw materials are cleaned and crush, being placed in 75 degree of edible alcohols and extracting 2.5 hours；Vacuum-concentrcted recycles
Ethyl alcohol, respectively obtain ginseng, Cordyceps militaris, lily, dried orange peel alcoholic extract；
3. the ginseng that step 2 is extracted, Cordyceps militaris, lily, dried orange peel alcoholic extract be added boiling water in extract 25 minutes,
Vacuum-concentrcted obtains the concentration alcohol medicinal extract of ginseng, Cordyceps militaris, lily, dried orange peel, and 80 mesh refined filtration of concentrated extract is removed slag；
4. by step 3 gained ginseng, the concentration alcohol medicinal extract low temperature drying of Cordyceps militaris, lily, dried orange peel, drying temperature 80
Degree, dry postcooling, respectively obtains the extract of ginseng, Cordyceps militaris, lily, dried orange peel；
5. the ginseng that mixing step three obtains, Cordyceps militaris, lily, dried orange peel extract, be added 20 parts of trehalose；
6. after being added and prepare the common auxiliary agent crushing of tablet, being sufficiently mixed uniformly, 40-80 mesh screens are crossed, tabletting is passed through
The direct machine of dry powder is tabletted.
Embodiment 4. is a kind of to have the capsule and preparation method thereof for improving immunity
1. weighing raw material (gram) by following weight ratios：
Ginseng 10, Cordyceps militaris 7, lily 24, dried orange peel 48；
2. after all raw materials are cleaned and crush, being placed in 75 degree of edible alcohols and extracting 2.5 hours；Vacuum-concentrcted recycles
Ethyl alcohol respectively obtains the alcoholic extract of ginseng, Cordyceps militaris, lily, dried orange peel；
3. by step 2 obtain ginseng, Cordyceps militaris, lily, dried orange peel alcoholic extract be added in boiling water and extract 25 minutes, very
The concentration alcohol medicinal extract for obtaining ginseng, Cordyceps militaris, lily, dried orange peel respectively is concentrated under reduced pressure in sky, and 80 mesh refined filtration of concentrated extract is removed slag.
4. by step 3 gained ginseng, the concentration alcohol medicinal extract low temperature drying of Cordyceps militaris, lily, dried orange peel, drying temperature 80
Degree, dry postcooling are added trehalose 20, are sufficiently mixed to obtain extract mixtures.
5. by the extract obtained mixture of step 4 and preparing after the common auxiliary agent of tablet crushes, is sufficiently mixed uniformly, mistake
40-80 mesh screens, it is dry by granulation, capsule is filled after the processes such as whole grain, is polished, is packed to get finished product.
Embodiment 5. is a kind of to have the granule and preparation method thereof for improving immunity
In addition to weighing raw material (gram) by following weight ratios：
Ginseng 5, Cordyceps militaris 10, lily 15, dried orange peel 60, remaining is same as Example 1.
Embodiment 6. is a kind of to have the granule and preparation method thereof for improving immunity
Raw material (gram) is weighed by following weight ratios：
Ginseng 13, Cordyceps militaris 3, lily 45, dried orange peel 30, remaining is same as Example 1.
Embodiment 7. is a kind of to have the granule and preparation method thereof for improving immunity
In addition to weighing raw material (gram) by following weight ratios：
Ginseng 15, Cordyceps militaris 3, lily 45, dried orange peel 25, remaining is same as Example 1.
Embodiment 8. is a kind of to have the oral solution and preparation method thereof for improving immunity
In addition to weighing raw material (gram) by following weight ratios：
Ginseng 3, Cordyceps militaris 12, lily 10, dried orange peel 75；Remaining is same as Example 1.
Improve immunity effect test embodiment.
Carry out the advantageous effect of the present invention is further explained pharmaceutical composition below by way of test example, these test examples include this
The edible effect observation for improving immunity experiment of the human body of oral liquid product prepared by inventive embodiments and Combined food of the present invention
Object strengthen immunity functional experiment development test example.
1. the edible effect observation for improving immunity experiment of the human body of oral liquid product prepared by the embodiment of the present invention
Time：On October 10,10 days~2016 October in 2015 eats oral solution prepared by the present invention in Shenzhen
Experiment, and tracking statistics has been carried out to body improvement.
Experiment purpose：Observation food compositions of the present invention improve the effect that immunocompromised tries out the immune function of crowd
1), subject
Children：Immunologic hypofunction, easy catching a cold, it is average monthly more than twice or in 1 year with pneumonia more than twice
Patient.
Elderly patients：Suffer from a variety of chronic diseases, is diagnosed as the patient of syndrome of deficiency of lung qi.
2), method of administration and the course for the treatment of：
The composition of the oral embodiment of the present invention 1, embodiment 5, embodiment 6, embodiment 7
Eating method is 1 bag every time 3 times a day, every bag of 9g.Open date is 1 year.
Control group：Cordyceps militaris capsule, 2. lilies+dried orange peel 1. (1: 1) capsule；Ginseng+lily 3. (1: 1) capsule.
3), observation method：
Into promoting circulation of blood, urine, feces routine test and the heart, pulmonary function test, and observe：Fever, aversion to cold, sniffle, pharyngeal disease
Shape and systemic conditions and physical examination temperature, pharyngeal tonsils, tongue picture pulse condition, the variations such as fingerprint carry out therapeutic evaluation respectively.
4), therapeutic evaluation
Table 1. is easy to catch cold, repeated cold, and rehabilitation is not easy after flu
Table 2.Senile lymph nodes of multiple sites enlargement, tubercle, the weak patient of night sweat
3. superficial gastritis of table, regular stomach account, the dim gas of stomach, dyspeptic patient
2, food compositions strengthen immunity function mouse experiment development test example of the present invention：
Test objective：Observation food compositions of the present invention influence the improvement result of hypoimmunity mice immune function
1), test material
1.1 reagents and drug and instrument
1.1.1 subject material and blank control：
Subject material：Food compositions prepared by embodiment 1, embodiment 5, embodiment 6, embodiment 7 are tested material.
Blank control：Distilled water
1.1.2 other reagents
ConA is purchased from upper Asteriidae bio tech ltd；
MTT：Sino American Biotec productions；
Sheep flood cell (SRBC)：Purchased from Beijing Bo Erxi Science and Technology Ltd.s)；
Microhemagglutination plate：Purchased from the Shanghai pools Scad Industrial Co., Ltd.；
CO2 incubators：Purchased from Shanghai Zhi Chu Instrument Ltd.；
India ink：Purchased from the Shanghai bio tech ltd Shi Feng；
Chicken red blood cell：Purchased from the prosperous bio tech ltd Changzhou Bei Yuan；
1.2 experimental animal：The cleaning grade Kunming germline provided by Changsha Kaifu District Dong Chuan Animal Sciences service department
Mouse, gender half male and half female, 18~22g of weight, each group 10.Feed, bedding and padding source are same as above.
1.3 animal room environmentals：20 DEG C~22 DEG C of temperature, humidity 45%~50%.
2), test basis：The Ministry of Public Health《Health food is examined and assessment technique specification》(2003 editions)
3), dosage selection and experiment packet：
3.1 dosage select：Mouse is administered by 10 times of human body recommended amounts.Give mouse 2.25g/Kg.bw、
4.50g/Kg、9.00g/Kg.Bw dosage (correspond to respectively human oral's dosage be 10,20,40mg/Kg weight) the present invention implement
The composition feeding of example 30 days.
3.2 experiment packet：Blank control group, 1 (embodiment 1) group of experiment, 2 (embodiment 5) groups of experiment, 3 (embodiments of experiment
6) group, 4 (embodiment 7) groups of experiment.
4), experimental method：Mouse is taken to be grouped into blank control group at random, trial drug group is according to dosage every with usage each group
Its gavage is primary, and carrying out every immune indexes to animal respectively after continuous 30d measures.
5), immune indexes measure project：
5.1, delayed allergy (DTH) (vola pedis thickens method)
Test method：Mouse is taken to be grouped at random：Blank control group, trial drug group, the daily gastric infusion of mouse 1 time, often
Secondary dosage is 0.3g/kg, and blank control group gives the distilled water for waiting capacity.Items are carried out after continuous 30d to animal respectively to exempt from
Epidemic disease index determining.In gavage the 26th day, giving mouse peritoneal to inject 2% (v/v) sheep red blood cell (SRBC), (SRBC was purchased from Beijing Bo Erxi
Science and Technology Ltd.) 0.2ml/ only carries out sensitization, and mouse peritoneal injects 2% (v/v) SRBC (0.2ml/ is per mouse) 4 days after sensitization,
Left back vola pedis thickness is measured, 20% (v/v) SRBC (20ul/ is per mouse) then, which is subcutaneously injected, in measuring point is attacked.And in
Left back foot plantar thickness is measured before attack and after attack for 24 hours, same position measures three times, is averaged.With vola pedis before and after attack
Thickness difference indicates the thickness of DTH, carries out variance analysis.Test result is shown in Table 4.
5.2 antibody-producting cells (PFc) detect
Test method：Mouse is taken to be grouped at random：Blank control group, trial drug group, the daily gastric infusion of mouse 1 time, often
Secondary dosage is 0.5g/kg, and blank control group gives the distilled water for waiting capacity.Items are carried out after continuous 30d to animal respectively to exempt from
Epidemic disease index determining.
Antibody-producting cell (PFc) detects：2%SRBC0 is injected in gavage to mouse peritoneal within the 25th day.2ml/ only, in exempting from
It puts to death within the 5th day after epidemic disease, solution takes spleen and prepares a concentration of 5x10 of splenocyte suspension RPMIl640 complete culture solutions adjustment splenocyte9
A/L.Agarose slide is prepared by program technic, in CO2Incubator (37 DEG C, 5%CO2) in incubate 1.5h after plus complement (be purchased from
Nanjing Dean bio tech ltd), then 1.5h is incubated, count the hemolysis plaque number formed on every thin agar layer slide.
Carry out variance analysis.Test result is shown in Table 5.
6), immune indexes measure project measurement result
6.1, the mouse delayed allergy (DTH) of dinitrofluorobenzene induction measures
The mouse delayed allergy (DTH) that table 4. carries out dinitrofluorobenzene induction using the composition of embodiment 1 is surveyed
Determine result (for 24 hours) (X ± S)
As a result it shows under this experiment condition, middle and high dosage group mouse swelling degree of the paw is all remarkably higher than control group (p ＜
0.05), difference has statistical significance.The ability of mouse delayed allergy can be improved in f high dose product in prompt.
6.2, antibody-producting cell (PFC) detection (X ± S)
Table 5. carries out antibody-producting cell (PFC) using the composition of embodiment 1 and detects (X ± S) result
As a result it is shown under this experimental condition, middle and high dosage group mouse hemolysis plaque number is significantly higher than control group (p respectively
＜ 0.05, p ＜ 0.01), middle and high dosage group and the result difference compareed are just statistically significant, prompt the implementation of middle and high dosage
The composition product of example 1 can significantly increase the antibody-producing capacity of mouse.
Conclusion：Relative to control group, orally administration mouse 2.25g/Kg.bw, 4.50g/Kg, 9.00g/Kg.bw dosage (divide
Do not correspond to human oral's dosage be 10,20,40mg/Kg weight) 1 product feeding of embodiment 30 days, 4.50g/Kg, 9.00g/
Kg.bw dosage can significantly improve the delayed allergy of mouse, antibody-producting cell number.It is detected and is implemented using same method
The composition that example 2, embodiment 3 and embodiment 4 obtain, the composition as a result obtained with method one is without significant difference.Prompt this hair
Bright composition has the function of strengthen immunity.
Claims (10)
1. a kind of food compositions, which is characterized in that the composition by raising immunity active mixture A and cell activity
Reinforcing agent B composition, the active mixture A for improving immunity by respectively from ginseng raw material, Cordyceps militaris raw material, dried orange peel raw material and
The extract composition that the extraction of lily raw material obtains.
2. according to claim 1 have the composition for improving immunity, which is characterized in that the cell activity increases
The preferred trehalose raw materials of strong agent B.
3. food compositions according to claim 1 or 2, which is characterized in that the ginseng raw material, Cordyceps militaris raw material, old
The weight ratio of skin raw material and lily raw material is 3~15 parts by weight of ginseng, 1~12 parts by weight of Cordyceps militaris, 10~45 weight of lily
Measure part, 25~75 parts by weight of dried orange peel, 10~40 parts by weight of active reinforcing agent B；Or the parts by weight preferably ginseng 5 of the raw material~
13 parts by weight, 3~10 parts by weight of Cordyceps militaris, 15~40 parts by weight of lily, 30~60 parts by weight of dried orange peel, 12~35 weight of trehalose
Part；Or 7~11 parts by weight of parts by weight preferably ginseng of the raw material, 5~7 parts by weight of Cordyceps militaris, 20~30 parts by weight of lily are old
35~50 parts by weight of skin, 15~30 parts by weight of trehalose；Or 7~9 parts by weight of parts by weight preferably ginseng of the raw material, Cordyceps militaris
4~7 parts by weight, 25~30 parts by weight of lily, 35~40 parts by weight of dried orange peel, 20~25 parts by weight of trehalose.
4. a kind of method preparing claims 1 to 3 any one of them food compositions, which is characterized in that including following step
Suddenly：
(1) the active mixture A for improving immunity is prepared；
Step A. sortings：Selected ginseng, Cordyceps militaris, lily, dried orange peel raw material；
Step B. is weighed：Weigh selected ginseng, Cordyceps militaris, lily, dried orange peel raw material
Step C. rinses raw material：Clear water is rinsed well；
Step D. is smashed；
Step E. alcoholic extractions and concentration：By the parts by weight ginseng of weighing, Cordyceps militaris, lily, dried orange peel raw material respectively in alcohol
It is concentrated after extraction, respectively obtains ginseng, Cordyceps militaris, lily, dried orange peel alcohol crude extract；
Step F：The ginseng of step 5, Cordyceps militaris, lily, dried orange peel alcohol crude extract are separately added into after being extracted in boiling water, point
It is not concentrated to give ginseng, Cordyceps militaris, lily, dried orange peel concentration alcohol medicinal extract；
Step G：Filtering；
Step H：Sterilizing, it is dry, mix active mixture A is saved backup；
(2) active reinforcing agent B is weighed；
(3) active reinforcing agent B is added to the active mixture A of immunity；
(4) auxiliary material is added and finished product is made；
Ginseng, Cordyceps militaris, lily, dried orange peel parts by weight of raw materials ratio described in step B are 3~15 parts by weight of ginseng, Cordyceps militaris 1~
12 parts by weight, 10~45 parts by weight of lily, 25~75 parts by weight of dried orange peel, 10~40 parts by weight of active reinforcing agent B；Or the original
5~13 parts by weight of parts by weight preferably ginseng of material, 3~10 parts by weight of Cordyceps militaris, 15~40 parts by weight of lily, 30~60 weight of dried orange peel
Measure part, 12~35 parts by weight of trehalose；Or 7~11 parts by weight of parts by weight preferably ginseng of the raw material, 5~7 weight of Cordyceps militaris
Part, 20~30 parts by weight of lily, 35~50 parts by weight of dried orange peel, 15~30 parts by weight of trehalose；Or the parts by weight of the raw material are excellent
7~9 parts by weight of ginseng of choosing, 4~7 parts by weight of Cordyceps militaris, 25~30 parts by weight of lily, 35~40 parts by weight of dried orange peel, trehalose 20
~25 parts by weight.
5. the preparation method of food compositions according to claim 4, which is characterized in that the alcoholic extraction is 70-85
Edible alcohol extraction is spent, the alcoholic extraction time is 1~4 hour, and the extracting in boiling water time is 10-50 minutes, described dense
It is condensed to vacuum-concentrcted, described to be filtered into -120 mesh of 50 mesh sieve refined filtration, the drying temperature is 40~90 DEG C.
6. the preparation method of food compositions according to claim 4, which is characterized in that the alcoholic extraction is 70-85
Edible alcohol extraction is spent, the alcoholic extraction time is 2~3 hours, and the extracting in boiling water time is 20-50 minutes, described dense
It is condensed to vacuum-concentrcted, described to be filtered into -100 mesh of 80 mesh sieve refined filtration, the drying temperature is 60~80 DEG C.
7. the application of food compositions described in claim 1, it is characterized in that improving hypoimmunity and sub-healty adults preparing
Application in the health food of group's immunity.
8. the application of food compositions described in claim 1, it is characterized in that in the guarantor for preparing conditioning digestive function ill symptoms
Application in health food.
9. the application of food compositions described in claim 1, it is characterized in that improving lymphatic system underactivity symptom preparing
Health food in application.
10. having the application for the composition for improving immunity, feature according to claim 7,8 or 9 any one of them
It is, the health food includes oral solution, capsule and tablet, beverage, biscuit.
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