CN108489787B - Blood test intelligence smear ware - Google Patents
Blood test intelligence smear ware Download PDFInfo
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- CN108489787B CN108489787B CN201810617313.XA CN201810617313A CN108489787B CN 108489787 B CN108489787 B CN 108489787B CN 201810617313 A CN201810617313 A CN 201810617313A CN 108489787 B CN108489787 B CN 108489787B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Abstract
The invention discloses an intelligent smear device for blood examination, which comprises a glass slide storage module, a sample adding module, a push piece module, a transmission module and a control module, wherein the sample adding module is arranged on the glass slide storage module; the invention utilizes the X-ray generator to scan the blood flowing channel, receives the blood by the high-resolution receiver, converts the blood by the digital-to-analog conversion unit, analyzes the viscosity of the blood by the analysis and operation unit, revises the angle and the force of the push sheet according to the characteristics of different blood viscosities, can make specific parameters for different blood and ensures the success rate of smear and later detection. In a word, the invention has the advantages of high smear efficiency, high accuracy and high success rate.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to an intelligent smear device for blood examination.
Background
With the development of modern medicine, blood examination becomes a routine means for testing many diseases, and according to data, plasma and internal suspended substances in blood gradually become main factors influencing the characteristics of blood samples. Other environmental factors, such as ambient temperature, humidity, shelf life and storage conditions, affect the structure of the blood film. For example, applying the same blood sample under the same smearing parameters, the blood film becomes shorter and thicker as the ambient temperature becomes lower. Conversely, as the ambient temperature increases, the longer and thinner the blood membrane becomes.
The traditional smear making process needs manual operation, the requirement is to uniformly coat the blood on the glass slide, the manual operation needs the experience of medical staff, and from the practical operation, the blood is difficult to be uniformly coated, the workload is large, the efficiency is low, and the single-time success rate is low.
Thus, in the prior art, a smear machine is presented to replace manual smearing, but the single smear machine can not improve the efficiency much than manual smearing. The batch smear machine is only used for experimental demonstration with low requirements on samples, and if the batch smear machine is actually used for hospital inspection, the uniform smearing parameters are adopted due to different characteristics of blood samples of different patients, so that the final effect of blood films is influenced. And cross contamination of blood is easily caused, which affects the accuracy of subsequent measurement.
Disclosure of Invention
Aiming at the technical problems, the invention provides an accurate intelligent smear device for blood examination.
The technical scheme of the invention is as follows: an intelligent smear device for blood examination, which comprises a glass slide storage module, a sample adding module, a push piece module, a transmission module and a control module;
the glass slide storage module comprises a storage bin and glass slides, glass slide clips made of magnetic materials are respectively clamped at four top corners of the glass slides and are stacked in the storage bin up and down, and an electric control door is arranged at the lower port of the storage bin and used for controlling and releasing the glass slides;
the sample adding module comprises a test tube rotating disc frame, a viscosity measurer and a manipulator, wherein the viscosity measurer is arranged on the right side of the slide glass storage module, a through hole is formed in the center of the longitudinal axis of the viscosity measurer, an X-ray generator is arranged on one side of the through hole, a high-resolution receiver opposite to the X-ray generator is arranged on the other side of the through hole, a first camera is embedded in the bottom of the viscosity measurer and used for monitoring whether blood drips on the slide glass or not, the test tube rotating disc frame is fixed above the viscosity measurer, a funnel containing blood to be measured is installed in the test tube rotating disc frame, and the manipulator is fixed to the top of the viscosity measurer and used for clamping or clamping the funnel;
the push piece module comprises a transverse support, a pressurizing telescopic rod, a push piece, a micro steering engine, a push piece sleeve removing mechanism, a push piece sleeve replacing mechanism, a sliding block and an electric reciprocating push rod, the transverse support is arranged on the right side of the sample adding module, the pressurizing telescopic rod is vertically connected to the front side face of the transverse support through the sliding block, the electric reciprocating push rod is fixed at the right end of the transverse support, the tail end of the electric reciprocating push rod is fixedly connected with the sliding block and used for driving the sliding block to reciprocate leftwards and rightwards along the transverse support so as to drive the push piece to smear blood, a second camera is arranged at the bottom of the sliding block, the rear end of the push piece is movably connected with the lower end of the pressurizing telescopic rod through a rotating shaft, a disposable push piece sleeve is sleeved at the front end of the push piece, cross contamination can be prevented, the measuring accuracy is improved, and the output shaft, the push piece sleeve removing mechanism is detachably connected below the left end of the transverse support, and the push piece sleeve replacing mechanism is detachably connected below the right end of the transverse support;
the conveying module comprises a horizontal electric conveyor and a magnetic block, the horizontal electric conveyor is positioned below the glass slide storage module, the sample adding module and the slide pushing module, and the magnetic block is arranged inside the horizontal electric conveyor and used for providing adsorption force for the glass slide clamp;
the control module comprises a servo drive unit, an image processing unit, a digital-to-analog conversion unit and an analysis operation unit, wherein the servo drive unit is used for controlling the operation of the slide glass storage module, the sample adding module, the slide pushing module and the transmission module; the image processing unit is used for processing the pictures shot by the first camera and the second camera; the digital-to-analog conversion unit is used for converting the X-ray information received by the high-resolution receiver into data; and the analysis operation unit is used for analyzing and operating the pictures and the data and feeding back the pictures and the data to the servo driving unit according to the result to perform instruction adjustment.
Further, the bottom of the circular cone portion of funnel is equipped with miniature one-way pressure valve, and the upper end of the pipeline portion of funnel is equipped with hollow sacculus, hollow sacculus upwards with miniature one-way pressure valve communicates with each other, communicates with each other with outside atmosphere downwards, circular cone portion upper end is equipped with sealed lid, and miniature one-way pressure valve is used for guaranteeing again under the external force condition that the inside blood of funnel can not leak and spill, and hollow sacculus is used for receiving external force extrusion to loosen the back, and inside formation is for the negative pressure cavity of circular cone portion to this opens miniature one-way pressure valve for the blood in the circular cone portion flows into hollow sacculus and caches, makes blood fall into on the slide glass through pipeline portion again.
Furthermore, the top of the viscosity measurer is provided with an extrusion mechanism, the extrusion mechanism comprises a first electric telescopic rod and a second electric telescopic rod, the first electric telescopic rod and the second electric telescopic rod are respectively and horizontally arranged at two ends of the through hole relatively and are electrically connected with the servo driving unit, and the first electric telescopic rod and the second electric telescopic rod are used for providing extrusion external force for the hollow balloon to form a negative pressure cavity.
Further, the mechanism is got rid of to the push away piece cover is including receiving box and micro-machine jack catch, the micro-machine jack catch is fixed on the inside wall at receiving box top, and the opening orientation the push away piece, the micro-machine jack catch with servo drive unit electric connection, the micro-machine jack catch is to wearing the push away piece cover of cover on the push away piece and removing, makes its automation fall into in the receiving box after loosening it again.
Further, the mechanism is changed to push jack cover includes storage box, baffle and compression spring, compression spring longitudinal fixation is in the interior bottom of storage box, baffle fixed connection in compression spring's upper end has placed on the baffle the push jack cover, the top of storage box is equipped with the export, the export orientation the push jack, the height of export equals the thickness of a push jack cover, utilizes compression spring's elasticity for the exit has the push jack cover to wait to be located all the time.
Furthermore, the inside of the storage bin is provided with a temperature control heater, the temperature control heater is electrically connected with the servo driving unit, and the temperature control heater is used for heating the glass slide at constant temperature, so that the glass slide has initial temperature, and the success rate of smear is favorably improved.
Further, the second camera is a 180-degree wide-angle camera, so that the removal of the push sheet sleeve can be assisted leftwards, the replacement of the push sheet sleeve can be assisted rightwards, the positions of the push sheet and blood can be monitored downwards, and the end of the smear is determined.
Furthermore, the electric control door is electrically connected with the servo driving unit and comprises an inner control door and an outer control door, the inner control door is arranged above the outer control door, the distance between the inner control door and the outer control door is equal to the thickness of the glass slide clamp, the inner control door is separated between the bottom glass slide and the glass slide above the bottom glass slide, when the outer control door is opened, only the bottommost glass slide can be ensured to be adsorbed to a belt of the horizontal electric conveyor through the magnetic glass slide clamp, and if the inner control door is not arranged, a plurality of glass slides are possibly adsorbed.
The working method of the invention is as follows:
the first step, clamping glass sheet clips made of magnetic materials at four top corners of the glass slide respectively, stacking the glass sheet clips in the storage bin up and down, opening the temperature control heater to set the temperature to be 25-30 ℃, and heating and preserving the heat for 20-30 min;
secondly, the servo driving unit controls the inner control door to be inserted between the bottom glass slide and the glass slide above the bottom glass slide, and then controls the outer control door to be opened, so that the glass clamp with the glass slide is adsorbed on a belt of the horizontal electric conveyor, and the outer control door is closed at the same time;
thirdly, the manipulator takes off a funnel from the test tube rotary disc frame and inserts the pipeline part of the funnel from the upper end of the through hole; the servo driving unit drives the horizontal electric conveyor to rotate, when the front part of the glass slide reaches the lower part of the funnel, the front part of the glass slide is fed back to the image processing unit according to the monitoring of the first camera, the distance is calculated through the analysis and calculation unit, the distance is fed back to the servo driving unit, and the movement of the horizontal electric conveyor is suspended;
fourthly, the first electric telescopic rod and the second electric telescopic rod are aligned with the hollow saccule to be extruded for 1-2s and then loosened to form a negative pressure cavity, so that the miniature one-way pressure valve is opened, blood in the conical part flows into the hollow saccule to be cached, and then the blood falls onto the glass slide through the pipeline part; when blood passes through the pipeline part, the X-ray generator scans the blood, the high-resolution receiver receives scanning signals and then transmits the scanning signals to the digital-to-analog conversion unit, the signals are converted into data, the viscosity of the blood is analyzed through the analysis and calculation unit, and the information of the viscosity of the blood is fed back to the servo driving unit and is used for revising the angle and the pressure of the push piece in the push piece module;
fifthly, when the first camera detects that blood drops exist on the glass slide, the blood drops are fed back to the servo driving unit through a feedback mechanism again, and the horizontal electric conveyor is started again to move until the second camera detects that the glass slide moves to the position right below the push piece and stops again;
sixthly, the pressurizing telescopic rod drives the push piece to retract upwards to the highest point, the micro steering engine rotates the push piece through the rotating shaft to be horizontally 90 degrees rightwards, the push piece enters from an outlet of the push piece sleeve replacing mechanism under the driving of the electric reciprocating push rod and under the positioning of the second camera, the push piece is withdrawn after being sleeved with the push piece sleeve, the micro steering engine rotates according to the angle set in the fourth step again under the positioning navigation of the second camera until the blood drop is contacted, the pressurizing telescopic rod applies pressure according to the set pressure, the pressurizing telescopic rod moves leftwards under the pushing of the electric reciprocating push rod, and the smear is finished after the second camera monitors the complete and clear head and tail shape; the pressurizing telescopic rod drives the push piece to retract upwards to the highest point again, the micro steering engine rotates the push piece through the rotating shaft to be horizontally 90 degrees leftwards, the push piece with the used push piece sleeve is aligned to the micro mechanical clamping jaw under the driving of the electric reciprocating push rod and the positioning of the camera II, and the used push piece sleeve is removed through the micro mechanical clamping jaw;
and seventhly, taking down the smeared glass slide, and repeating the steps from one step to six until the smearing is finished.
Compared with the prior art, the invention has the beneficial effects that:
(1) in the process of flowing of blood in a pipeline, the blood is scanned by using an X-ray generator, received by a high-resolution receiver and then converted by a digital-to-analog conversion unit, the viscosity of the blood is analyzed by using an analysis and operation unit, the angle and the force of a push sheet are revised according to the characteristics of different blood viscosities, specific parameters can be made for different blood, and the success rate of smear and later detection is ensured;
(2) the funnel can realize the integration of blood storage and dripping, wherein the micro one-way pressure valve is used for ensuring that the blood in the funnel cannot leak under the condition of external force, the hollow saccule is used for forming a negative pressure cavity relative to the conical part after being extruded and loosened by the external force, so that the micro one-way pressure valve is opened, the blood in the conical part flows into the hollow saccule for caching, and then the blood falls into the glass slide through the pipeline part; the trouble and inaccuracy of manual dripping are avoided, and the independent design can avoid the cross contamination of blood;
(3) the push sheet is provided with the disposable push sheet sleeve, and the removal and replacement operations of the push sheet sleeve can be completed by self under the automatic operation, so that the cross contamination of blood is avoided;
(4) the magnetic block is arranged in the horizontal electric conveyor, and can provide an adsorption force for the glass slide clamp made of a magnetic material through a belt, so that the glass slide is fixed and prevented from displacing in a smearing process;
(5) the temperature control heater in the storage bin is used for heating the glass slide at constant temperature to enable the glass slide to have initial temperature, and is favorable for improving the success rate of smear.
In a word, the invention has the advantages of high smear efficiency, high accuracy and high success rate.
Drawings
FIG. 1 is a schematic view of the structure of a slide storage module, a sample application module, a slide pushing module and a transfer module according to the present invention;
FIG. 2 is a system block diagram of the present invention;
FIG. 3 is a cross-sectional view of the push plate sleeve removal mechanism of the present invention;
fig. 4 is a sectional view of the push jack cover changing mechanism of the present invention.
Wherein, 1-glass slide storage module, 10-storage bin, 100-temperature control heater, 11-glass slide, 12-glass slide clamp, 13-electric control door, 130-inner control door, 131-outer control door, 2-sample loading module, 20-test tube rotary table rack, 21-viscosity measuring device, 210-extrusion mechanism, 211-electric expansion rod I, 212-electric expansion rod II, 22-mechanical arm, 23-through hole, 24-X ray generator, 25-high resolution receiver, 26-camera head I, 27-funnel, 27 a-cone part, 27 b-pipeline part, 270-minitype one-way pressure valve, 271-hollow balloon, 272-sealing cover, 3-push piece module, 30-transverse support, 31-pressurizing expansion rod, 32-push piece, 33-micro steering engine, 34-push piece sleeve removing mechanism, 340-receiving box, 341-micro mechanical claw, 35-push piece sleeve replacing mechanism, 350-storing box, 351-partition plate, 352-compression spring, 353-outlet, 36-sliding block, 37-electric reciprocating push rod, 38-second camera, 39-rotating shaft, 310-push piece sleeve, 4-transmission module, 40-horizontal electric conveyor, 41-magnetic block, 5-control module, 50-servo drive unit, 51-image processing unit, 52-digital-to-analog conversion unit and 53-analysis operation unit.
Detailed Description
The following description of the preferred embodiments of the present invention is provided as a preferred embodiment of the present invention, but the practice and protection of the present invention is not limited thereto.
As shown in fig. 2, an intelligent smear device for blood test comprises a slide glass storage module 1, a sample adding module 2, a push piece module 3, a transmission module 4 and a control module 5;
as shown in fig. 1, the slide glass storage module 1 comprises a storage bin 10 and slide glass 11, wherein glass clips 12 made of magnetic materials are respectively clamped at four top corners of the slide glass 11 and are stacked up and down in the storage bin 10, and an electric control door 13 is arranged at the lower port of the storage bin 10 and used for controlling and releasing the slide glass 11; as shown in fig. 1, the electric control door 13 is electrically connected to the servo driving unit 50, and includes an inner control door 130 and an outer control door 131, the inner control door 130 is disposed above the outer control door 131, and the distance between the inner control door 130 and the outer control door 131 is equal to the thickness of the slide holder 12, the inner control door 130 is spaced between the bottom slide 11 and the slide 11 above the bottom slide, when the outer control door 131 is opened, it can be ensured that only the bottom slide 11 is attracted to the belt of the horizontal electric conveyor 40 through the magnetic slide holder 12, and if there is no inner control door 130, there is a high possibility that a plurality of slides 11 are attracted. The temperature control heater 100 is arranged in the storage bin 10, the temperature control heater 100 is electrically connected with the servo driving unit 50, and the temperature control heater 100 is used for heating the glass slide 11 at a constant temperature to enable the glass slide to have an initial temperature, so that the success rate of smearing is improved.
As shown in fig. 1, the sample adding module 2 includes a test tube carousel rack 20, a viscosity measuring device 21, and a manipulator 22, wherein the viscosity measuring device 21 is disposed at the right side of the slide glass storage module 1, a through hole 23 is disposed at the center of the longitudinal axis of the viscosity measuring device 21, an X-ray generator 24 is disposed at one side of the through hole 23, a high-resolution receiver 25 opposite to the X-ray generator 24 is disposed at the other side of the through hole 23, a first camera 26 is embedded in the bottom of the viscosity measuring device 21 for monitoring whether blood is dripped onto the slide glass 11, the test tube carousel rack 20 is fixed above the viscosity measuring device 21, a funnel 27 containing blood to be measured is installed in the test tube carousel rack 20, and the manipulator 22 is fixed at the top of the viscosity measuring device 21; as shown in fig. 1, a micro one-way pressure valve 270 is disposed at the bottom of the conical portion 27a of the funnel 27, a hollow balloon 271 is disposed at the upper end of the tube portion 27b of the funnel 27, the hollow balloon 271 is upwardly communicated with the micro one-way pressure valve 270 and downwardly communicated with the outside atmosphere, a sealing cover 272 is disposed at the upper end of the conical portion 27a, the micro one-way pressure valve 270 is used for ensuring that the blood in the funnel 27 does not leak under the condition of external force, the hollow balloon 271 is used for forming a negative pressure cavity relative to the conical portion 27a after being squeezed and released by the external force, so as to open the micro one-way pressure valve 270, so that the blood in the conical portion 27a flows into the hollow balloon 271 for buffering, and then the blood falls onto the slide 11 through the. As shown in fig. 1, an extruding mechanism 210 is disposed at the top of the viscosity measuring device 21, the extruding mechanism 210 includes a first electric telescopic rod 211 and a second electric telescopic rod 212, the first electric telescopic rod 211 and the second electric telescopic rod 212 are respectively disposed at two ends of the through hole 23 in a relatively horizontal manner and electrically connected to the servo driving unit 50, and the first electric telescopic rod 211 and the second electric telescopic rod 212 are configured to provide an extruding external force to the hollow balloon 271 so as to form a negative pressure cavity.
As shown in fig. 1, the push sheet module 3 comprises a transverse support 30, a pressurizing telescopic rod 31, a push sheet 32, a micro steering engine 33, a push sheet sleeve removing mechanism 34, a push sheet sleeve replacing mechanism 35, a slide block 36 and an electric reciprocating push rod 37, the transverse support 30 is arranged at the right side of the sample adding module 2, the pressurizing telescopic rod 31 is vertically connected to the front side surface of the transverse support 30 through the slide block 36, the electric reciprocating push rod 37 is fixed at the right end of the transverse support 30, and the tail end is fixedly connected with the sliding block 36 for driving the sliding block 36 to reciprocate left and right along the transverse bracket 30, and then drive push jack 32 to carry out the smear processing to blood, the bottom of slider 36 is equipped with second camera 38, and second camera 38 is 180 degrees wide angle cameras, both can assist the removal of push jack cover 310 left, can assist the change of push jack cover 310 to the right again, can also monitor the position of push jack 32 and blood downwards to and confirm that the smear is ended. The rear end of the push sheet 32 is movably connected with the lower end of the pressurizing telescopic rod 31 through a rotating shaft 39, a disposable push sheet sleeve 310 is sleeved at the front end of the push sheet 32, cross contamination can be prevented, and measurement accuracy is improved, an output shaft of the micro steering engine 33 is connected with one end of the rotating shaft 39 and used for adjusting the rotating angle of the push sheet 32, a push sheet sleeve removing mechanism 34 is detachably connected below the left end 30 of the transverse support, as shown in fig. 3, the push sheet sleeve removing mechanism 34 comprises a receiving box 340 and a micro mechanical clamping jaw 341, the micro mechanical clamping jaw 341 is fixed on the inner side wall of the top of the receiving box 340, the opening faces the push sheet 32, the micro mechanical clamping jaw 341 is electrically connected with the servo driving unit 50, and the micro mechanical clamping jaw 341 removes the push sheet sleeve 310 which is sleeved on the push sheet 32 in a penetrating mode and then automatically falls into the receiving box 340 after being. The push sheet sleeve replacing mechanism 35 is detachably connected below the right end of the transverse bracket 30; as shown in fig. 4, the push sheet sleeve replacing mechanism 35 includes a storage box 350, a partition 351 and a compression spring 352, the compression spring 352 is longitudinally fixed at the inner bottom of the storage box 350, the partition 351 is fixedly connected to the upper end of the compression spring 352, the push sheet sleeve 310 is placed on the partition 351, an outlet 353 is arranged at the top of the storage box 350, the outlet 353 faces the push sheet 32, the height of the outlet 353 is equal to the thickness of one push sheet sleeve 310, and the push sheet sleeve 310 is always kept at the outlet 353 by the elasticity of the compression spring 352.
As shown in fig. 1, the transfer module 4 includes a horizontal electric conveyor 40 and a magnetic block 41, the horizontal electric conveyor 40 is located below the slide storage module 1, the sample adding module 2 and the slide pushing module 3, and the magnetic block 41 is arranged inside the horizontal electric conveyor 40 and is used for providing an adsorption force to the slide clamp 12;
as shown in fig. 2, the control module 5 includes a servo driving unit 50, an image processing unit 51, a digital-to-analog conversion unit 52, and an analysis and operation unit 53, wherein the servo driving unit 50 is used for controlling the operation of the slide glass storage module 1, the sample adding module 2, the slide pushing module 3, and the transfer module 4; the image processing unit 51 is used for processing the pictures shot by the first camera 26 and the second camera 38; the digital-to-analog conversion unit 52 is used for converting the X-ray information received by the high-resolution receiver 25 into data; the analysis and operation unit 53 is used for performing analysis and operation on the pictures and the data, and feeding back the results to the servo driving unit 50 for instruction adjustment.
The working method of the embodiment comprises the following steps:
firstly, clamping glass clips 12 made of magnetic materials at four top corners of a glass slide 11 respectively, stacking the glass clips in a storage bin 10 up and down, opening a temperature control heater 100, setting the temperature to be 28 ℃, and heating and preserving the temperature for 25 min;
secondly, the servo driving unit 50 controls the inner control door 130 to be inserted between the bottom slide 11 and the slide 11 above the bottom slide, and then controls the outer control door 131 to be opened, so that the glass clip 12 with the slide 11 is adsorbed on the belt of the horizontal electric conveyor 40, and simultaneously closes the outer control door 131;
thirdly, the manipulator 22 takes off a funnel 27 from the test tube rotating tray frame 20 and inserts the tube part 27b thereof from the upper end of the through hole 23; the horizontal electric conveyor 40 is driven to rotate by the servo driving unit 50, when the front part of the slide glass 11 reaches the lower part of the funnel 27, the front part is fed back to the image processing unit 51 according to the monitoring of the first camera 26, the distance is calculated by the analysis and calculation unit 53, the distance is fed back to the servo driving unit 50, and the movement of the horizontal electric conveyor 40 is suspended;
fourthly, the first electric telescopic rod 211 and the second electric telescopic rod 212 are aligned with the hollow balloon 271 to be extruded for 1 to 2 seconds and then loosened to form a negative pressure cavity, so that the micro one-way pressure valve 270 is opened, blood in the conical part 27a flows into the hollow balloon 271 to be buffered, and then the blood falls onto the glass slide 11 through the pipeline part 27 b; when blood passes through the pipeline part 27b, the X-ray generator 24 scans the blood, the high-resolution receiver 25 receives the scanning signal and transmits the scanning signal to the digital-to-analog conversion unit 52, the signal is converted into data, the viscosity of the blood is analyzed through the analysis and calculation unit 53, and the information of the viscosity of the blood is fed back to the servo driving unit 50 and is used for revising the angle and the pressure of the push sheet 32 in the push sheet module 3;
fifthly, when the first camera 26 detects that blood drops exist on the slide 11, the blood drops are fed back to the servo driving unit 50 through a feedback mechanism again, so that the horizontal electric conveyor 40 is started again to move until the second camera 38 detects that the slide 11 moves to the position right below the push piece 32 and stops again;
sixthly, the pressurizing telescopic rod 31 drives the push piece 32 to retract upwards to the highest point, the micro steering engine 33 rotates the push piece 32 through the rotating shaft 39 to be horizontally 90 degrees rightwards, the push piece 32 enters from the outlet 353 of the push piece sleeve replacing mechanism 35 under the driving of the electric reciprocating push rod 37 and the positioning of the second camera 38, the push piece is pulled out after the push piece sleeve 310 is sleeved, the micro steering engine 33 rotates according to the angle set in the step four again under the positioning navigation of the second camera 38 until the blood drops are contacted, the pressurizing telescopic rod 31 applies pressure according to the set pressure, the pressurizing telescopic rod 31 moves leftwards under the pushing of the electric reciprocating push rod 37, and the smear is finished after the second camera 38 monitors the complete and clear head tail shape; the pressurizing telescopic rod 31 drives the push sheet 32 to retract upwards to the highest point again, the micro steering engine 33 rotates the push sheet 32 through the rotating shaft 39 to be horizontal for 90 degrees leftwards, the push sheet 32 with the used push sheet sleeve 310 is aligned to the micro mechanical claw 341 under the driving of the electric reciprocating push rod 37 and the positioning of the camera II 38, and the used push sheet sleeve 310 is removed by using the micro mechanical claw 341;
and seventhly, taking down the slide glass 11 after the smearing is finished, and repeating the steps from one step to six until the smearing is finished.
Effect verification:
subject: 100 blood samples to be smeared are taken, each sample is divided into 3 equal parts, and the 100 samples are smeared by adopting an embodiment group, a control group and a reference group respectively, wherein the embodiment group adopts the blood smear device disclosed by the invention, the control group adopts the blood smear device disclosed by the publication number CN207488042U, and the reference group selects an artificial smear optimal template as a comparison reference of the experiment group and the control group.
The experimental method comprises the following steps: 1) the smear operation time is completely finished by comparing the example group and the control group in a timing mode; 2) the appearance of the smear samples of the example group and the control group is evaluated by a human observation method, and the smear samples are qualified if the head and the tail are obvious, or are unqualified if the head and the tail are obvious; 3) and comparing the smear detection results of the example group and the control group with the detection results of the reference group, and evaluating the accuracy.
The experimental results are as follows: as shown in table 1:
table 1: experimental results of example group, control group and reference group
As can be seen from Table 1, the blood smear device of the present invention has higher efficiency, the qualification rate is 100%, and the accuracy rate is as high as 99.5%.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (6)
1. An intelligent smear device for blood test is characterized by comprising a glass slide storage module (1), a sample adding module (2), a push piece module (3), a transmission module (4) and a control module (5);
the glass slide storage module (1) comprises a storage bin (10) and glass slides (11), glass slide clamps (12) made of magnetic materials are clamped at four top corners of each glass slide (11) respectively and are stacked in the storage bin (10) up and down, and an electric control door (13) is arranged at the lower port of the storage bin (10);
the sample adding module (2) comprises a test tube rotary tray frame (20), a viscosity measurer (21) and a manipulator (22), the viscosity measurer (21) is arranged on the right side of the slide glass storage module (1), a through hole (23) is formed in the center of the longitudinal axis of the viscosity measurer (21), an X-ray generator (24) is arranged on one side of the through hole (23), a high-resolution receiver (25) opposite to the X-ray generator (24) is arranged on the other side of the through hole (23), a first camera (26) is embedded in the bottom of the viscosity measurer (21), the test tube rotary table rack (20) is fixed above the viscosity measurer (21), a funnel (27) containing blood to be measured is arranged in the test tube rotary table rack (20), the manipulator (22) is fixed at the top of the viscosity measurer (21), for clamping the funnel (27) in or out of the through-going hole (23);
the push sheet module (3) comprises a transverse support (30), a pressurizing telescopic rod (31), a push sheet (32), a micro steering engine (33), a push sheet sleeve removing mechanism (34), a push sheet sleeve replacing mechanism (35), a sliding block (36) and an electric reciprocating push rod (37), the transverse support (30) is arranged on the right side of the sample adding module (2), the pressurizing telescopic rod (31) is vertically connected to the front side surface of the transverse support (30) through the sliding block (36), the electric reciprocating push rod (37) is fixed at the right end of the transverse support (30), the tail end of the electric reciprocating push rod is fixedly connected with the sliding block (36) and used for driving the sliding block (36) to reciprocate left and right along the transverse support (30), a second camera (38) is arranged at the bottom of the sliding block (36), and the rear end of the push sheet (32) is movably connected with the lower end of the pressurizing telescopic rod (31) through a rotating shaft (39, the front end of the push piece (32) is sleeved with a disposable push piece sleeve (310), an output shaft of the micro steering engine (33) is connected with one end of the rotating shaft (39) and used for adjusting the rotating angle of the push piece (32), the push piece sleeve removing mechanism (34) is detachably connected below the left end of the transverse support (30), and the push piece sleeve replacing mechanism (35) is detachably connected below the right end of the transverse support (30);
the conveying module (4) comprises a horizontal electric conveyor (40) and a magnetic block (41), the horizontal electric conveyor (40) is positioned below the glass slide storage module (1), the sample adding module (2) and the slide pushing module (3), and the magnetic block (41) is arranged inside the horizontal electric conveyor (40) and used for providing adsorption force for the glass slide clamp (12);
the control module (5) comprises a servo drive unit (50), an image processing unit (51), a digital-to-analog conversion unit (52) and an analysis operation unit (53), wherein the servo drive unit (50) is used for controlling the operation of the slide glass storage module (1), the sample adding module (2), the slide pushing module (3) and the transmission module (4); the image processing unit (51) is used for processing pictures shot by the first camera (26) and the second camera (38); the digital-to-analog conversion unit (52) is used for converting the X-ray information received by the high-resolution receiver (25) into data; the analysis operation unit (53) is used for analyzing and operating the pictures and the data and feeding back the pictures and the data to the servo driving unit (50) according to results to perform instruction adjustment;
the electric control door (13) is electrically connected with the servo drive unit (50) and comprises an inner control door (130) and an outer control door (131), the inner control door (130) is arranged above the outer control door (131), the distance between the inner control door (130) and the outer control door (131) is equal to the thickness of the glass slide clamp (12), the inner control door (130) is separated between the bottom glass slide (11) and the glass slide (11) above the bottom glass slide, and when the outer control door (131) is opened, only the bottom glass slide (11) can be adsorbed onto a belt of the horizontal electric conveyor (40) through the magnetic glass slide clamp (12);
the temperature control device is characterized in that a temperature control heater (100) is arranged in the storage bin (10), and the temperature control heater (100) is electrically connected with the servo driving unit (50).
2. The intelligent smear machine for blood test as claimed in claim 1, wherein the bottom of the conical part (27 a) of the funnel (27) is provided with a miniature one-way pressure valve (270), the upper end of the pipeline part (27 b) of the funnel (27) is provided with a hollow balloon (271), the hollow balloon (271) is communicated with the miniature one-way pressure valve (270) upwards and is communicated with the external atmosphere downwards, and the upper end of the conical part (27 a) is provided with a sealing cover (272).
3. The intelligent blood examination smear machine according to claim 2, wherein the viscosity measuring device (21) is provided with an extrusion mechanism (210) at the top, the extrusion mechanism (210) comprises a first electric telescopic rod (211) and a second electric telescopic rod (212), the first electric telescopic rod (211) and the second electric telescopic rod (212) are respectively horizontally arranged at two ends of the through hole (23) relatively and are electrically connected with the servo driving unit (50).
4. The intelligent smear device for blood test as claimed in claim 1, wherein the smear removing mechanism (34) comprises a receiving box (340) and a micro mechanical claw (341), the micro mechanical claw (341) is fixed on the inner side wall of the top of the receiving box (340) and opens towards the smear (32), the micro mechanical claw (341) is electrically connected with the servo driving unit (50).
5. The intelligent smear device for blood test as claimed in claim 1, wherein the push sheet sleeve replacing mechanism (35) comprises a storage box (350), a partition (351) and a compression spring (352), the compression spring (352) is longitudinally fixed at the inner bottom of the storage box (350), the partition (351) is fixedly connected at the upper end of the compression spring (352), the push sheet sleeve (310) is placed on the partition (351), the top of the storage box (350) is provided with an outlet (353), the outlet (353) faces to the push sheet (32), and the height of the outlet (353) is equal to the thickness of one push sheet sleeve (310).
6. The intelligent blood test smear machine as claimed in claim 1 wherein the second camera (38) is a 180 degree wide angle camera.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810617313.XA CN108489787B (en) | 2018-06-15 | 2018-06-15 | Blood test intelligence smear ware |
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| CN201810617313.XA CN108489787B (en) | 2018-06-15 | 2018-06-15 | Blood test intelligence smear ware |
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| CN108489787B true CN108489787B (en) | 2020-08-04 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109668763A (en) * | 2019-01-03 | 2019-04-23 | 桂林优利特医疗电子有限公司 | A kind of blood pushing piece auto-feeder |
| CN114729898A (en) * | 2019-11-29 | 2022-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | Smear preparation device, sample analysis system and method |
| CN111551418B (en) * | 2020-04-01 | 2023-11-07 | 郑州中普医疗器械有限公司 | Slide pushing device |
| CN114076699A (en) * | 2020-08-14 | 2022-02-22 | 爱威科技股份有限公司 | Blood push sheet manufacturing device |
| CN115144247B (en) * | 2022-09-02 | 2022-11-15 | 深圳明灏生物科技有限公司 | Smear device for liquid-based thin-layer cell detection |
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