CN108485943A - A kind of cancer related gene mutation high-sensitivity detecting method - Google Patents
A kind of cancer related gene mutation high-sensitivity detecting method Download PDFInfo
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- CN108485943A CN108485943A CN201810223271.1A CN201810223271A CN108485943A CN 108485943 A CN108485943 A CN 108485943A CN 201810223271 A CN201810223271 A CN 201810223271A CN 108485943 A CN108485943 A CN 108485943A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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Abstract
The invention discloses a kind of cancer related genes to be mutated high-sensitivity detecting method, including detecting box body, it is equipped with test tube holder on detection box body, liquid getting device, temperature control knob, reactive tank, heat-preservation cotton, detect box framework, detect box drawer slot, temperature control plate, rubber spacer, Reagent Tube, separate slot, temperature-sensitive module, power supply, temperature controller, microcontroller, circuit control module, it is internally provided with multiple detection box drawer slots in detection kit, the temperature control of each detection box drawer slot is different, pass through the color change in reactive tank after reaction, to detect the catastrophe of different temperatures section cancer gene, and it can detect the most suitable abrupt temp range of cancer gene, kind is simple, quickly, the method of highly sensitive detection cancer cell.
Description
Technical field
The present invention relates to gene mutation high-sensitivity detecting method technical field, specially a kind of cancer related gene mutation
High-sensitivity detecting method.
Background technology
Tumour seriously threatens the life security of people, it has also become and it is a kind of common fatal disease, hair
Sick rate has raised trend year by year, the detection of cancer to have become one of the hot spot of whole world research, realizes that tumour is accurate, sensitive,
Simplicity detection has very important significance for the treatment of disease.
Currently, the detection means taken is mostly enzyme-linked immunosorbent assay, this method passes through between detection tumour cell marker
Detection tumour cell is connect, therefore sensitivity is relatively low, it is difficult to which early detection simultaneously prevents.
Invention content
The purpose of the present invention is to provide a kind of cancer related genes to be mutated high-sensitivity detecting method, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve the above object, the present invention provides the following technical solutions：A kind of highly sensitive inspection of cancer related gene mutation
Survey method, including detection box body, the detection box body are equipped with detection box framework, and the detection box framework is internally provided with
Box drawer slot is detected, detection box drawer slot is internally provided with reactive tank, and the bottom right side of the detection box framework is revolved equipped with temperature control
Button, temperature control system, temperature control knob are connect with temperature control system, and the top of the temperature control system is equipped with liquid getting device, Reagent Tube, the inspection
The upper side for surveying box body is equipped with box cover, and box cover is internally provided with rubber spacer, and the inside of the box cover is equipped with close to right side
Separate slot.
Preferably, the bottom of the detection box drawer slot is equipped with temperature control plate, and temperature control plate is connect with temperature control system.
Preferably, the reactive tank be internally provided with 10% cow's serum, culture medium, amino acid, sodium bicarbonate, reaction
The temperature controlling range of slot is 35 DEG C -45 DEG C, and the outside of the reactive tank is equipped with heat-preservation cotton, and the top of reactive tank is equipped with transparent close
Capping, two reactive tanks detected above box body are the formula that opens up, and detect four reactive tanks below box body and are
Drawing and pulling type.
Preferably, the temperature control system is equipped with microcontroller, and the left side connecting pin of the microcontroller is equipped with power supply, temperature control
The other end of device, the temperature controller is equipped with temperature-sensitive module, and the other end connecting pin of the microcontroller is equipped with circuit control module, electricity
Road control module is connect with temperature controller.
Preferably, it is TMB solution, diphenylamines, methyl green, pyrrole Luo Hong equipped with color developing agent in the Reagent Tube.
Preferably, the outside of the Reagent Tube is equipped with test tube holder, and test tube holder uses polymeric foam layer, Reagent Tube
Bottom is equipped with sponge bed course.
Preferably, a kind of cancer related gene mutation high-sensitivity detecting method includes the following steps：
A, by the inside of reactive tank be placed with 10% cow's serum, culture medium, amino acid, sodium bicarbonate,；
B, the reactive tank equipped with culture solution is installed in detection box framework, is adjusted in each reactive tank using temperature control knob
Temperature controlling range, make culture solution preheat 10min；
C, cancer cell is respectively put into the reactive tank of different temperatures and is cultivated, incubation time 80min-90min；
D, diphenylamines, methyl green, pyrrole Luo Hong these detection reagents are respectively dropped into the inside of reactive tank, detection reagent will be instilled
Reactive tank reacts 10min-15min after closing, and then takes out the color change observed in each reactive tank.
Compared with prior art, the beneficial effects of the invention are as follows：
In the present invention, the temperature control of each detection box drawer slot is different, passes through the color in reactive tank after reaction
Variation, to detect the catastrophe of different temperatures section cancer gene, and can detect the most suitable mutation of cancer gene
Temperature range, the method for simple, quick, the highly sensitive detection cancer cell of kind.
Description of the drawings
Fig. 1 is present invention detection box body structural schematic diagram；
Fig. 2 is built-in circuit schematic diagram of the present invention.
In figure：1, box body is detected；2, test tube holder；3, liquid getting device；4, temperature control knob；5, reactive tank；6, heat-preservation cotton；7、
Detect box framework；8, box drawer slot is detected；9, temperature control plate；10, rubber spacer；11, Reagent Tube；12, separate slot；13, temperature-sensitive module；
14, power supply；15, temperature controller；16, microcontroller；17, circuit control module；18, temperature control system.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
- Fig. 2 is please referred to Fig.1, the present invention provides a kind of technical solution：A kind of highly sensitive detection of cancer related gene mutation
Method, including detection box body 1, detection box body 1 are equipped with detection box framework 7, and detection box framework 7 is internally provided with detection box
Drawer slot 8, detection box drawer slot 8 are internally provided with reactive tank 5, reactive tank 5 be internally provided with 10% cow's serum, culture medium,
Amino acid, sodium bicarbonate, the temperature controlling range of reactive tank 5 is 35 DEG C -45 DEG C, and the outside of reactive tank 5 is equipped with heat-preservation cotton 6, instead
The top of slot 5 is answered to be equipped with transparent sealing lid, two reactive tanks 5 of 1 top of detection box body are the formula that opens up, and detect box body
Four reactive tanks of 1 lower section are drawing and pulling type.
In the present invention, the bottom right side of detection box framework 7 is equipped with temperature control knob 4, temperature control system 18, temperature control knob 4 and temperature
Control system 18 connects, and the top of temperature control system 18 is equipped with liquid getting device 3, Reagent Tube 11, and the upper side of detection box body 1 is equipped with box
Lid, box cover are internally provided with rubber spacer 10, and the inside of box cover is equipped with separate slot 12 close to right side, avoids in detection box framework 7
Temperature controls.
In the present invention, the bottom of detection box drawer slot 8 is equipped with temperature control plate 9, and temperature control plate 9 is connect with temperature control system 18, temperature control
System 18 is equipped with microcontroller 16, and the left side connecting pin of microcontroller 16 is equipped with power supply 14, temperature controller 15, the other end of temperature controller 15
Equipped with temperature-sensitive module 13, the other end connecting pin of microcontroller 16 is equipped with circuit control module 17, circuit control module 17 and temperature control
Device 15 connects, and adjusts temperature control knob 4 to control the electric current that power supply 14 accesses circuit, to control the temperature range of temperature control plate 9.
The outside of Reagent Tube 11 is equipped with test tube holder 2, and test tube holder 2 uses polymeric foam layer, the bottom of Reagent Tube 11
Equipped with sponge bed course, it is TMB solution, diphenylamines, methyl green, pyrrole Luo Hong that color developing agent, which is housed, in Reagent Tube 11, by diphenylamines, first
Base is green, pyrrole Luo Hong these detection reagents are respectively dropped into the inside of reactive tank 5, will instill detection reagent reactive tank 5 close after it is anti-
10min-15min is answered, the color change observed in each reactive tank is then taken out.
In the present invention, a kind of cancer related gene mutation high-sensitivity detecting method includes the following steps：
A, by the inside of reactive tank 5 be placed with 10% cow's serum, culture medium, amino acid, sodium bicarbonate,；
B, the reactive tank 5 equipped with culture solution is installed in detection box framework 7, each reaction is adjusted using temperature control knob 4
Temperature controlling range in slot 5 makes culture solution preheat 10min；
C, cancer cell is respectively put into the reactive tank 5 of different temperatures and is cultivated, incubation time 80min-90min；
D, diphenylamines, methyl green, pyrrole Luo Hong these detection reagents are respectively dropped into the inside of reactive tank 5, detection reagent will be instilled
Reactive tank 5 close after react 10min-15min, then take out observe each reactive tank in color change.
In the present invention, each detection box drawer slot temperature control it is different, by reactive tank after reaction
Color change to detect the catastrophe of different temperatures section cancer gene, and can detect that cancer gene is most suitable
Abrupt temp range, the method for simple, quick, the highly sensitive detection cancer cell of kind.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of cancer related gene is mutated high-sensitivity detecting method, including detection box body（1）, it is characterised in that：It is described
Detect box body（1）It is equipped with detection box framework（7）, the detection box framework（7）Be internally provided with detection box drawer slot（8）,
Detect box drawer slot（8）Be internally provided with reactive tank（5）, the detection box framework（7）Bottom right side be equipped with temperature control knob
（4）, temperature control system（18）, temperature control knob（4）With temperature control system（18）Connection, the temperature control system（18）Top be equipped with take liquid
Device（3）, Reagent Tube（11）, the detection box body（1）Upper side be equipped with box cover, box cover is internally provided with rubber spacer
（10）, the inside of the box cover is equipped with separate slot close to right side（12）.
2. a kind of cancer related gene according to claim 1 is mutated high-sensitivity detecting method, it is characterised in that：It is described
Detect box drawer slot（8）Bottom be equipped with temperature control plate（9）, temperature control plate（9）With temperature control system（18）Connection.
3. a kind of cancer related gene according to claim 1 is mutated high-sensitivity detecting method, it is characterised in that：It is described
Reactive tank（5）Be internally provided with 10% cow's serum, culture medium, amino acid, sodium bicarbonate, reactive tank（5）Temperature control model
It is 35 DEG C -45 DEG C to enclose, the reactive tank（5）Outside be equipped with heat-preservation cotton（6）, reactive tank（5）Top be equipped with transparent sealing lid,
The detection box body（1）Two reactive tanks of top（5）To open up formula, box body is detected（1）Four reactions of lower section
Slot is drawing and pulling type.
4. a kind of cancer related gene according to claim 1 is mutated high-sensitivity detecting method, it is characterised in that：It is described
Temperature control system（18）It is equipped with microcontroller（16）, the microcontroller（16）Left side connecting pin be equipped with power supply（14）, temperature controller
（15）, the temperature controller（15）The other end be equipped with temperature-sensitive module（13）, the microcontroller（16）Other end connecting pin be equipped with
Circuit control module（17）, circuit control module（17）With temperature controller（15）Connection.
5. a kind of cancer related gene according to claim 1 is mutated high-sensitivity detecting method, it is characterised in that：It is described
Reagent Tube（11）In equipped with color developing agent be TMB solution, diphenylamines, methyl green, pyrrole Luo Hong.
6. a kind of cancer related gene according to claim 1 is mutated high-sensitivity detecting method, it is characterised in that：It is described
Reagent Tube（11）Outside be equipped with test tube holder（2）, test tube holder（2）Using polymeric foam layer, Reagent Tube（11）Bottom
Equipped with sponge bed course.
7. a kind of cancer related gene according to claim 1 is mutated high-sensitivity detecting method, it is characterised in that：Detection
Method includes the following steps：
A, by reactive tank（5）Inside be placed with 10% cow's serum, culture medium, amino acid, sodium bicarbonate,；
B, by the reactive tank equipped with culture solution（5）It is installed to detection box framework（7）It is interior, using temperature control knob（4）It adjusts each
A reactive tank（5）Interior temperature controlling range makes culture solution preheat 10min；
C, cancer cell is respectively put into the reactive tank of different temperatures（5）It is inside cultivated, incubation time 80min-90min；
D, diphenylamines, methyl green, pyrrole Luo Hong these detection reagents are respectively dropped into reactive tank（5）Inside, by instill detection examination
The reactive tank of agent（5）10min-15min is reacted after closing, then takes out the color change observed in each reactive tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810223271.1A CN108485943A (en) | 2018-03-19 | 2018-03-19 | A kind of cancer related gene mutation high-sensitivity detecting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810223271.1A CN108485943A (en) | 2018-03-19 | 2018-03-19 | A kind of cancer related gene mutation high-sensitivity detecting method |
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| Publication Number | Publication Date |
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| CN108485943A true CN108485943A (en) | 2018-09-04 |
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| CN201810223271.1A Withdrawn CN108485943A (en) | 2018-03-19 | 2018-03-19 | A kind of cancer related gene mutation high-sensitivity detecting method |
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Application publication date: 20180904 |
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