CN206173324U - WST 1 cell proliferation and cytotoxicity detect reagent box - Google Patents
WST 1 cell proliferation and cytotoxicity detect reagent box Download PDFInfo
- Publication number
- CN206173324U CN206173324U CN201621218108.9U CN201621218108U CN206173324U CN 206173324 U CN206173324 U CN 206173324U CN 201621218108 U CN201621218108 U CN 201621218108U CN 206173324 U CN206173324 U CN 206173324U
- Authority
- CN
- China
- Prior art keywords
- fixed plate
- box body
- fixedly connected
- wst
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a WST 1 cell proliferation and cytotoxicity detect reagent box, comprises a box bod, first fixed plate of fixedly connected with and second fixed plate on the inner wall of box body, and the second fixed plate is located the top of first fixed plate, there is the lid at the top of box body through hinge swing joint, a side fixedly connected with colorimetric card of lid, the last fixed surface of second fixed plate is connected with the guide bar. The utility model discloses a set up box body, first fixed plate, second fixed plate and 96 orifice plates, if the cell needs long -time the cultivation, can be to adding an amount of water in the box body, reduce by 96 orifice plates around the round evaporate easily for the cell is normally bred, and solved and examined time measuring using 96 orifice plates to carry out cell proliferation and cytotoxicity, if the cell culture time is longer, the round evaporates easily around 96 orifice plates, is unfavorable for cell proliferation, possibly still can lead to cell death, influences the problem of testing result.
Description
Technical field
This utility model is related to biological technical field, and specially a kind of WST-1 cells are bred and cytotoxicity detectable
Box.
Background technology
WST-1 is the upgrading substitute products of MTT, has been compared substantially with MTT or other MTT similar products such as XTT and MTS etc.
Advantage.First, the formazan that MTT is generated by Intramitochondrial some dehydrogenase reduction is not water miscible, it is desirable to have special
Fixed lysate is dissolving；And the formazan that WST-1 and XTT, MTS are produced is water miscible, can save follow-up molten
Solution step.Secondly, the formazan that WST-1 is produced is more more soluble than the formazan that XTT and MTS is produced.Again, WST-1 ratios
XTT and MTS are more stable, make experimental result more stable.In addition, WST-1 and MTT, XTT etc. are compared, the range of linearity it is wider and
The advantages of sensitivity is higher.But when being detected using 96 orifice plates, if the cell culture time is longer, due to 96 orifice plates week
Make a circle and be easiest to evaporation, be unfavorable for that cell is bred, it is possible to also result in cell death, affect testing result.
Utility model content
(1) technical problem for solving
For the deficiencies in the prior art, this utility model provides WST-1 cells and breeds and citotoxicity detection kit,
Solve when cell propagation and cytotoxicity detection is carried out using 96 orifice plates, if the cell culture time is longer, 96 orifice plates week
Make a circle easily evaporation, is unfavorable for that cell is bred, it is possible to also result in cell death, affects the problem of testing result.
(2) technical scheme
For achieving the above object, this utility model provides following technical scheme：A kind of WST-1 cells propagation and cytotoxicity
Detection kit, including box body, are fixedly connected with the first fixed plate and the second fixed plate on the inwall of the box body, and second consolidates
Fixed board is located at the top of the first fixed plate, and the top of the box body is connected with lid, the side of the lid by hinge
Face is fixedly connected with colorimetric card, and the upper surface of second fixed plate is fixedly connected with guide post, and the guide post is flexibly connected
In the through hole that 96 orifice plate upper surfaces are opened up, the one side of the box body is fixedly connected with outlet pipe, and outlet pipe and box body
Inside is connected, and the water side of the outlet pipe is threaded with closure.
The one side of the box body offers groove, and the internal activity of the groove is connected with drawer, and the one of the drawer
Side is fixedly connected with pulling buckle, and the first demarcation strip, the side of first demarcation strip are fixedly connected with the inwall of the drawer
Face is fixedly connected with the second demarcation strip.
Preferably, the quantity of first demarcation strip is three, and the quantity of the second demarcation strip is nine, and three first point
Drawer is divided into nine equal-sized placing troughs by dividing plate and nine the second demarcation strips.
Preferably, first fixed plate is fixedly connected with sealing ring with the junction of box body.
Preferably, the size of 96 orifice plate is adapted with the size of box body.
Preferably, first fixed plate and the vertical dimension of the second fixed plate are eight centimetres.
(3) beneficial effect
This utility model provides WST-1 cells and breeds and citotoxicity detection kit.Possesses following beneficial effect：
This utility model is by arranging box body, the first fixed plate, the second fixed plate and 96 orifice plates, when being detected, root
Determine time length according to the type of cell and the experimental conditions such as density of cell, if cell needs long-time to cultivate, can be to box
Add a certain amount of water, reduction to make a circle in 96 orifice plate weeks and easily evaporate in body so that cell normal proliferative, solve and using 96
When orifice plate carries out cell propagation and cytotoxicity detection, if the cell culture time is longer, made a circle in 96 orifice plate weeks easily evaporation,
It is unfavorable for that cell is bred, it is possible to also result in cell death, affects the problem of testing result.
Description of the drawings
Fig. 1 is this utility model structural representation；
Fig. 2 is the first fixed plate structure schematic diagram of the present utility model.
In figure：1- box bodys, the fixed plates of 2- first, the fixed plates of 3- second, 4- hinges, 5- lids, 6- colorimetric cards, 7- are oriented to
Bar, 8-96 orifice plates, 9- through holes, 10- outlet pipes, 11- closures, 12- grooves, 13- drawers, 14- pulling buckles, the demarcation strips of 15- first,
The demarcation strips of 16- second, 17- sealing rings.
Specific embodiment
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of this utility model, rather than whole
Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment for being obtained, belongs to the scope of this utility model protection.
As shown in Figure 1-2, this utility model provides technical scheme：A kind of WST-1 cells propagation and cytotoxicity detection examination
Agent box, including box body 1, are fixedly connected with the first fixed plate 2 and the second fixed plate 3, and the second fixed plate 3 on the inwall of box body 1
It is eight centimetres positioned at the vertical dimension of the top of the first fixed plate 2, the first fixed plate 2 and the second fixed plate 3, the first fixed plate 2
Sealing ring 17 is fixedly connected with the junction of box body 1, prevents water from flowing into the lower floor of box body 1, pollute reagent, increased structure
Practicality, the top of box body 1 is connected with lid 5 by hinge 4, and the one side of lid 5 is fixedly connected with colorimetric card 6,
WST-1 is analogous to the compound of MTT, in the presence of electronics coupled reagent, can be by Intramitochondrial some dehydrogenations
Enzyme reduction generates orange-yellow formazan, and cell propagation is more much faster, then color is deeper；Cytotoxicity is bigger, then color is got over
It is shallow, and colorimetric card 6 is compared, and judges the value-added speed and cytotoxicity of cell, the upper surface of the second fixed plate 3 is fixed and connected
Guide post 7 is connected to, guide post 7 is movably connected in the through hole 9 that the upper surface of 96 orifice plate 8 is opened up, size and the box body 1 of 96 orifice plates 8
Size be adapted, the quantity of guide post 7 is four, is fixed 96 orifice plates 8 by guide post 7, prevents 96 orifice plates 8 from rocking,
96 orifice plates 8 can also be removed, be changed or cleaned, it is simple and convenient, and the second fixed plate 3 is used for 96 orifice plates 8 of support, leads to
Often cell proliferation experiment adds 100 microlitres of 2000 cells per hole, and addition 100 microlitres 5000 is thin per hole for cytotoxicity experiment
Born of the same parents, the number of concrete cell used by per hole, need to determine according to factors such as the size of cell, the speeds of cell proliferation rate, press
Needs are according to the facts tested, 0-10 microlitre of specific medicine irritation is cultivated and given, 10 microlitres of WST-1 solution are added per hole, if
The volume of culture of starting is 200 microlitres, then need to add 20 microlitres of WST-1 solution, and other situations by that analogy, can be with having added phase
Cell culture fluid and WST-1 solution should be measured but do not add the hole of cell as blank, continue to incubate in cell culture incubator
0.5-4 hours are educated, for majority of case, just, time length is close according to the type of cell and cell for incubation 1-2 hours
Depending on the experimental conditions such as degree, if cell needs long-time to cultivate, a certain amount of water can be added in box body 1, reduce by 96 orifice plates 8
Make a circle in week easily evaporation so that cell normal proliferative, the one side of box body 1 is fixedly connected with outlet pipe 10, and outlet pipe 10
It is connected with the inside of box body 1, and outlet pipe 10 is located at the top of the first fixed plate 2, opening closure 11 can be by box body 1
Water release, the water side of outlet pipe 10 is threaded with closure 11, and this test kit can be used for the inductions such as cytokine
Cell propagation detection, it is also possible to detect for the cytotoxicity that cancer therapy drug etc. is induced cell toxic reagent, or some medicines
The cell growth inhibition detection of induction.
The one side of box body 1 offers groove 12, and the internal activity of groove 12 is connected with drawer 13, the one side of drawer 13
Pulling buckle 14 is fixedly connected with, the first demarcation strip 15 is fixedly connected with the inwall of drawer 13, the one side of the first demarcation strip 15 is consolidated
Surely the second demarcation strip 16 is connected with, the quantity of the first demarcation strip 15 is three, the quantity of the second demarcation strip 16 is nine, and three
First demarcation strip 15 and nine the second demarcation strips 16 by drawer 13 points be nine equal-sized placing troughs, drawer 13 is used to deposit
WST-1 reagents and electronics coupled reagent, when needing configuration solution to be tested, 5 milliliters of electronics coupled reagents are added to
In WST-1 powder, be completely dissolved WST-1 solution.
When using, open drawer 13 and take out WST-1 reagents and electronics coupled reagent, 5 milliliters of electronics coupled reagents are added
To in WST-1 powder, be completely dissolved WST-1 solution, then by cell add 96 orifice plates 8 in, cultivated, time length root
Depending on according to the type of cell and the experimental conditions such as density of cell, if cell needs long-time to cultivate, can add in box body 1
A certain amount of water, 96 orifice plate of reduction makes a circle and easily evaporate for 8 weeks so that cell normal proliferative, and this test kit is detected very just
Victory, only completes, it is not necessary to washed cell, it is not necessary to collect without isotope, all of detecting step is used in the orifice plate 8 of same 96
Cell, also need not go to dissolve formazan using additional step, can be used for the detection of batch samples.
To sum up can obtain, this utility model is by arranging box body 1, the first fixed plate 2, the second fixed plate 3 and 96 orifice plates 8, solution
Determine when cell propagation and cytotoxicity detection is carried out using 96 orifice plates 8, if the cell culture time is longer, 96 orifice plates 8 weeks
Make a circle easily evaporation, is unfavorable for that cell is bred, it is possible to also result in cell death, affects the problem of testing result.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or deposit between operating
In any this actual relation or order.And, term " including ", "comprising" or its any other variant are intended to
Nonexcludability is included, so that a series of process, method, article or equipment including key elements not only will including those
Element, but also including other key elements being not expressly set out, or also include for this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions, the key element for being limited by sentence "including a ...", it is not excluded that
Also there is other identical element in process, method, article or equipment including the key element.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out various changes in the case of without departing from principle of the present utility model and spirit, repaiies
Change, replace and modification, scope of the present utility model is defined by the appended claims and the equivalents thereof.
Claims (5)
1. a kind of WST-1 cells are bred and citotoxicity detection kit, including box body (1), it is characterised in that：The box body
(1) the first fixed plate (2) and the second fixed plate (3) are fixedly connected with inwall, and the second fixed plate (3) is fixed positioned at first
The top of plate (2), the top of the box body (1) is connected with lid (5), the one side of the lid (5) by hinge (4)
Colorimetric card (6) is fixedly connected with, the upper surface of second fixed plate (3) is fixedly connected with guide post (7), the guide post
(7) it is movably connected in the through hole (9) that 96 orifice plates (8) upper surface is opened up, the one side of the box body (1) is fixedly connected with water outlet
Manage (10), and outlet pipe (10) is connected with the inside of box body (1), the water side of the outlet pipe (10) is threaded with sealing
Lid (11)；
The one side of the box body (1) offers groove (12), and the internal activity of the groove (12) is connected with drawer (13), institute
The one side for stating drawer (13) is fixedly connected with pulling buckle (14), and on the inwall of the drawer (13) the first demarcation strip is fixedly connected with
(15), the one side of first demarcation strip (15) is fixedly connected with the second demarcation strip (16).
2. a kind of WST-1 cells according to claim 1 are bred and citotoxicity detection kit, it is characterised in that：Institute
The quantity for stating the first demarcation strip (15) is three, and the quantity of the second demarcation strip (16) is nine, and three the first demarcation strips (15)
Drawer (13) is divided into into nine equal-sized placing troughs with nine the second demarcation strips (16).
3. a kind of WST-1 cells according to claim 1 are bred and citotoxicity detection kit, it is characterised in that：Institute
State the first fixed plate (2) and be fixedly connected with sealing ring (17) with the junction of box body (1).
4. a kind of WST-1 cells according to claim 1 are bred and citotoxicity detection kit, it is characterised in that：Institute
The size for stating 96 orifice plates (8) is adapted with the size of box body (1).
5. a kind of WST-1 cells according to claim 1 are bred and citotoxicity detection kit, it is characterised in that：Institute
It is eight centimetres that the first fixed plate (2) is stated with the vertical dimension of the second fixed plate (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621218108.9U CN206173324U (en) | 2016-11-11 | 2016-11-11 | WST 1 cell proliferation and cytotoxicity detect reagent box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621218108.9U CN206173324U (en) | 2016-11-11 | 2016-11-11 | WST 1 cell proliferation and cytotoxicity detect reagent box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206173324U true CN206173324U (en) | 2017-05-17 |
Family
ID=58684953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621218108.9U Expired - Fee Related CN206173324U (en) | 2016-11-11 | 2016-11-11 | WST 1 cell proliferation and cytotoxicity detect reagent box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206173324U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108485943A (en) * | 2018-03-19 | 2018-09-04 | 吴娜 | A kind of cancer related gene mutation high-sensitivity detecting method |
| CN113278672A (en) * | 2021-04-20 | 2021-08-20 | 广东纽唯质量技术服务有限公司 | Medical instrument and method for detecting cytotoxicity of medical material |
-
2016
- 2016-11-11 CN CN201621218108.9U patent/CN206173324U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108485943A (en) * | 2018-03-19 | 2018-09-04 | 吴娜 | A kind of cancer related gene mutation high-sensitivity detecting method |
| CN113278672A (en) * | 2021-04-20 | 2021-08-20 | 广东纽唯质量技术服务有限公司 | Medical instrument and method for detecting cytotoxicity of medical material |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206173324U (en) | WST 1 cell proliferation and cytotoxicity detect reagent box | |
| CN102279264A (en) | Preparation process for chip with 48 malignant tumor marker antibodies | |
| CN103193746A (en) | Specific probe substrate of catechol-O-methyltransgerase and application thereof | |
| CN102220284A (en) | Fluorescent cell model for screening of antitumor drugs, labeling method and application thereof | |
| CN205808905U (en) | A kind of counting chamber with degassing function | |
| CN103018401A (en) | Aerial seeding forest sandy soil organic carbon reserve measurement method | |
| CN206208716U (en) | A kind of flowing counting pool for being integrated with reagent | |
| CN204757886U (en) | Antibacterial ring of diameter dipperstick | |
| CN205484363U (en) | Small -size concentration gradient drug sensitive test test paper | |
| CN214703624U (en) | Nitrogen leaching loss measuring device | |
| CN204679509U (en) | A kind of aristolochic acid A rapid detection card | |
| CN209669196U (en) | A kind of CCK-8 cell proliferation detecting kit | |
| CN106526029A (en) | Detection method and detection card of thiamphenicol | |
| CN212688021U (en) | Novel cell counter | |
| CN207423973U (en) | A kind of anti-pollution test card | |
| JPS6240284A (en) | Cell cultivation vessel | |
| Raysyan | Lateral Flow ImmunoAssays for the detection of bisphenol A emissions from plastic materials | |
| CN206450589U (en) | A kind of disposable two point sample position counting chamber | |
| CN215162830U (en) | Cancer cell medicine detection device | |
| CN105738614B (en) | A kind of method that Mouse bone marrow erythrocyte systematic function is evaluated using flow cytometry analysis | |
| CN204509331U (en) | Integral type count enable culture dish | |
| Blumenthal et al. | Isotope assay of histamine release | |
| CN203534651U (en) | Flow-controllable multi-mode flow detection device with digital display function | |
| CN219830995U (en) | Stainless steel gas capacity detection tube for aerosolization powder process | |
| CN104849460A (en) | Stable hepatitis C virus core antigen enzyme conjugate diluent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170517 Termination date: 20181111 |