CN108469367A - A kind of centrifugal plasma separation CD - Google Patents
A kind of centrifugal plasma separation CD Download PDFInfo
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- CN108469367A CN108469367A CN201810450960.6A CN201810450960A CN108469367A CN 108469367 A CN108469367 A CN 108469367A CN 201810450960 A CN201810450960 A CN 201810450960A CN 108469367 A CN108469367 A CN 108469367A
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- pond
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- detaches
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- 238000000926 separation method Methods 0.000 title abstract description 11
- 210000002381 plasma Anatomy 0.000 claims abstract description 75
- 210000004369 blood Anatomy 0.000 claims abstract description 40
- 239000008280 blood Substances 0.000 claims abstract description 40
- 238000001514 detection method Methods 0.000 abstract description 17
- 238000012360 testing method Methods 0.000 abstract description 8
- 238000005119 centrifugation Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 22
- 210000002798 bone marrow cell Anatomy 0.000 description 2
- 208000014951 hematologic disease Diseases 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 208000019838 Blood disease Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000093 cytochemical effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007723 die pressing method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 208000018706 hematopoietic system disease Diseases 0.000 description 1
- 208000007475 hemolytic anemia Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Urology & Nephrology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention discloses a kind of centrifugal plasmas to detach CD, including CD, the separator being located on CD and the quantitative collection device being located on CD.Separator includes being located at whole blood pond, blood plasma pond and conduit, and one end of conduit is connect with whole blood pond, and the other end is connect with blood plasma pond.Quantitative collection device includes quantitative pond, the run-off being connect with quantitative pond and connecting tube, and one end of connecting tube is connect with quantitative pond, and the other end is connect with blood plasma pond.Originally make invention in such a way that centrifugation is combined with quantitative separating, the blood plasma of more parts of equivalent can be isolated from a whole blood, effectively shorten disengaging time, avoid the various errors being likely to occur in artificial separation, detection speed and accuracy of detection can also be improved simultaneously, further increases the level of blood testing.
Description
Technical field
The present invention relates to blood testing fields, and in particular to a kind of centrifugal plasma separation CD.
Background technology
Clinical blood detection can be divided into blood generally detect, the test in laboratory of hemolytic anemia, bone marrow cell detect,
Bracket for blood grouping and cross match blood test.It can detect that the hematology of common hematological diseases holds sign.
One of the most common is that blood generally detects(That is blood routine).I.e. to red blood cell, leucocyte and blood platelet these three
The amount and matter of system are detected and analyze.It is also wanted with the relevant detection of various diseases if blood disease is in addition to blood routine to be detected
Carry out bone marrow cell detection, blood cyto-chemical stain analysis etc.;It is main for the blood testing project of hepatopathy and kidney patient
Detection etc. including liver function, renal function;And for some communicable diseases, the detection of antibodies in blood is the weight of diagnosis
It will foundation.
Traditional detection means is detected for some index or certain specific substance after sample collection, is examined
Ranging sequence is more, and the detection of a routine needs more more equipment operations of people, and detection time is long, and testing cost is high, is its outstanding feature.
Especially it is certain need to be detected using reagent in the case of, a variety of interference such as sample error, instrumental error, human error
Factor can all influence testing result.Although the spreading speed of automatic detection system is obviously accelerated in recent years, easily tens
Ten thousand purchase costs even up to a million, general hospital and testing agency are difficult to bear.Especially blood testing parameter is various, inspection
Measurement equipment inevitably has specific aim again, also exacerbates this trend from another point of view.
Invention content
The present invention is intended to provide it is a kind of can simultaneous quantitative isolate the centrifugal plasma separation CD of more points of blood plasma.
The present invention adopts the following technical scheme that:
A kind of centrifugal plasma detaches CD, including CD, the separator that is located on CD and is located at quantifying on CD and adopts
Acquisition means;
The separator includes being located at whole blood pond, blood plasma pond and conduit;
One end of the conduit is connect with whole blood pond, and the other end is connect with blood plasma pond;
The quantitative collection device includes quantitative pond, the run-off being connect with quantitative pond and connecting tube;
One end of the connecting tube is connect with quantitative pond, and the other end is connect with blood plasma pond.
As further solution:The leading flank in the whole blood pond and trailing flank are made of a plurality of curve;Arbitrarily
The center of curvature of one curve is located on the axis of CD.
As further solution:The leading flank in the blood plasma pond and trailing flank are made of a plurality of curve;Arbitrarily
The center of curvature of one curve is located on the axis of CD.
As further solution:The center of curvature of the axis of the conduit is located on the axis of CD.
As further solution:On the direction vertically with CD, there are the curved surfaces that one passes through catheter shaft line;Institute
It states curved surface and whole blood pond is divided into top half and lower half portion;The top half is close to the part of CD axis;On described
Half part and the volume ratio of lower half portion are 3:2.
As further solution:The run-off is located at quantitative pond close to the side of cd centre.
As further solution:The quantitative pond surrounds the axis circular array of CD;The quantitative pond is successively
Series connection;The only corresponding quantitative pond connection of the run-off.
As further solution:The separator further includes diversion pipe;The both ends of the diversion pipe respectively with
Adjacent quantitative pond connection;The center of curvature of the diversion pipe is located on the axis of CD.
As further solution:The quantitative collection device further includes aerial drainage pond;
The aerial drainage pond is connect with the last one run-off;
One end of the connecting tube is connect with first blood plasma pond.
As further solution:The minimum range of the aerial drainage pond and cd centre is more than in run-off and CD
The minimum range of the heart.
The good effect that the present invention generates is as follows:
The present invention sub-argument can go out more points of blood plasma, separative efficiency higher in a blood plasma mask work.In use, blood is noted
Enter whole blood pond, under the influence of centrifugal force, blood is separated into blood plasma and haemocyte, is then retransferred in plasma flow to blood plasma pond
Into each quantitative pond.In this way, first separation operation can isolate more points of blood plasma, disengaging time can be greatly shortened, is carried
High separating efficiency.
The more points of Plasma volumes equalizations that the present invention isolates avoid tradition separation after the completion again by manually-operated separation
Work, improves the precision of Quantitative Separation.The volume in each quantitative pond is equal on CD, and blood plasma is entered by blood plasma pond
During each quantitative pond, extra part is automatically transferred in run-off and aerial drainage pond, to ensure that each is quantitative
Chi Jun is filled by blood plasma.More points of blood plasma of the equivalent isolated just can be directly used for detection operation, effectively shorten detection
Time particular avoids the secondary pollution being likely to occur in artificial lock out operation and quantitative error, can give subsequent detection
Work provides solid guarantee.
Description of the drawings
Fig. 1 is the structural diagram of the present invention;
Wherein:11 whole blood ponds, 12 blood plasma ponds, 13 conduits, 21 quantitative ponds, 22 run-offs, 23 connecting tubes, 3 diversion pipes, 4 aerial drainage ponds.
Specific implementation mode
With reference to Fig. 1, invention is further explained.
The present invention adopts the following technical scheme that:
A kind of centrifugal plasma detaches CD, including CD, the separator that is located on CD and is located at quantifying on CD and adopts
Acquisition means;
The separator includes being located at whole blood pond 11, blood plasma pond 12 and conduit 13;
One end of the conduit 13 is connect with whole blood pond 11, and the other end is connect with blood plasma pond 12;
The quantitative collection device includes quantitative pond 21, the run-off 22 being connect with quantitative pond 21 and connecting tube 23;
One end of the connecting tube 23 is connect with quantitative pond 21, and the other end is connect with blood plasma pond 12.
As further solution:The leading flank in the whole blood pond 11 and trailing flank are made of a plurality of curve;Appoint
The center of curvature of one curve of meaning is located on the axis of CD.
As further solution:The leading flank in the blood plasma pond 12 and trailing flank are made of a plurality of curve;Appoint
The center of curvature of one curve of meaning is located on the axis of CD.
As further solution:The center of curvature of the axis of the conduit 13 is located on the axis of CD.
As further solution:On the direction vertically with CD, there are the curved surfaces that one passes through 13 axis of conduit;
The curved surface divides in whole blood pond 11 for top half and lower half portion;The top half is close to the part of CD axis;Institute
The volume ratio for stating top half and lower half portion is 3:2.
As further solution:The run-off 22 is located at quantitative pond 21 close to the side of cd centre.
As further solution:The quantitative pond 21 surrounds the axis circular array of CD;The quantitative pond 21
It is sequentially connected in series;The only corresponding quantitative pond 21 of the run-off 22 connects.
As further solution:The separator further includes diversion pipe 3;Distinguish at the both ends of the diversion pipe 3
It is connect with adjacent quantitative pond 21;The center of curvature of the diversion pipe 3 is located on the axis of CD.
As further solution:The quantitative collection device further includes aerial drainage pond 4;
The aerial drainage pond 4 is connect with the last one run-off 22;
One end of the connecting tube 23 is connect with first blood plasma pond 12.
As further solution:The aerial drainage pond 4 and the minimum range of cd centre are more than run-off 22 and light
The minimum range of disk center.
With reference to the actual course of work, invention is further explained.
The present invention needs to coordinate dedicated centrifugally operated equipment, each operation that CD is store in the equipment required
Rotation time and velocity of rotation.The centrifugally operated equipment repeats no more not within the protection domain of the application.
CD mentioned in the present invention is made of high molecule plastic, and whole blood pond 11 and blood plasma pond 12 etc. are made using molding
Make, connecting tube 23 and conduit 13 etc. are made of accurate die pressing or etching, are integrated on a CD.
In use, whole blood is injected into whole blood pond 11, then start centrifugally operated equipment, CD starts to rotate.From
Under the action of mental and physical efforts, whole blood is separated into blood plasma and haemocyte, and the volume of blood plasma is about the 60% of volume of whole blood, the body of haemocyte
Product is about the 40% of whole blood.
The blood plasma isolated is flow to by conduit 13 in blood plasma pond 12, is then flow to respectively along connecting tube 23 each
In a quantitative pond 21.With the injection of blood plasma, quantitative pond 21 is gradually filled.An air part in quantitative pond 21 is transferred to down
In one quantitative pond, another part is extruded in run-off 22, while extra part blood plasma is also flow in run-off 22.
As the air in run-off 22 is compressed, pressure also gradually increases.After pressure increase to a certain extent in run-off 22,
Blood plasma does not enter back into.Blood plasma starts to flow and repeat the process to second quantitative pond 21 at this time, until whole quantitative ponds 21
Filled by blood plasma.
The air sent in quantitative pond 21 is finally transferred completely into aerial drainage pond 4, it is extra go out blood plasma be equally transferred to and let out
It flows in pond 4.
So far, blood plasma mask work is completed, and separation number is determined by the quantity in quantitative pond 21, because of each quantitative pond 21
Volume be all equal, therefore what is isolated is all equivalent per a blood plasma.It is taken out from quantitative pond 21 can
Carry out subsequent detection process.
From the point of view of analysis, a sample can make multiple detection samples, substantially reduce sample time, simultaneously
The sample of more parts of equivalent in turn avoids the error that subsequent artefacts divide sample to generate, and can significantly improve subsequent accuracy of detection.
It makes and being explained further below for concrete structure of the invention:
The leading flank in whole blood pond 11 is just proximate to the curved surface of cd centre, forms the bent curvature of a curve parallel with CD of the curved surface
Center is respectively positioned on the axis of CD, and such purpose is for haemocyte, the distribution of centrifugal force be it is uniform, will not
There is the phenomenon that centrifugal force is bigger than normal or less than normal in a certain region, causes blood separation part unbalance, in turn result in and isolate
Haemocyte situation on the high side in blood plasma.The structure in blood plasma pond 12 is similar with the structure in whole blood pond 11, and details are not described herein again.
The center of curvature of 13 axis of conduit is located on the axis of CD, and the blood plasma that it can be made internal at the uniform velocity flows, and avoids
Blood plasma is layered.
The junction in quantitative pond 21 and blood plasma pond 12 is located at the trailing flank in blood plasma pond 12, and reason is that blood plasma enters
It moves to trailing flank behind blood plasma pond 12, the residual quantity in blood plasma pond 12 can be preferably minimized so under the influence of centrifugal force,
Improve the utilization rate of blood plasma.
It is connected by diversion pipe 3 between quantitative pond 21, the radius of curvature of each section of diversion pipe 3 is equal, and each section
The center of curvature of diversion pipe 3 is respectively positioned on the axis of CD, can ensure the plasma flow rate phase in each section of diversion pipe 3 in this way
Deng avoiding the occurrence of the too fast situation for leading to blood plasma deficiency in adjacent quantitative pond 21 of local velocity.
It is compared with run-off 22, aerial drainage pond 4 is farther at a distance from cd centre, is to make the last one run-off in this way
Extra blood plasma can be smoothly transferred in aerial drainage pond 4 in 22, avoid the occurrence of that back pressure is excessive to cause blood plasma that can not shift.Simultaneously
In order to further decrease the air pressure in aerial drainage pond 4, a stomata or gas-guide tube can be increased on aerial drainage pond 4, make its with it is outer
Boundary's air communicates, and ensures that the air pressure in aerial drainage pond 4 is equal with external atmospheric pressure always.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations, although
Present invention has been described in detail with reference to the aforementioned embodiments, it will be understood by those of ordinary skill in the art that, still may be used
To modify to the technical solution recorded in previous embodiment or equivalent replacement of some of the technical features, and
These modifications or replacements, the spirit and model of technical solution of the embodiment of the present invention that it does not separate the essence of the corresponding technical solution
It encloses.
Claims (10)
1. a kind of centrifugal plasma detaches CD, it is characterised in that:Including CD, the separator being located on CD and it is located at light
Quantitative collection device on disk;
The separator includes being located at whole blood pond(11), blood plasma pond(12)And conduit(13);
The conduit(13)One end and whole blood pond(11)Connection, the other end and blood plasma pond(12)Connection;
The quantitative collection device includes quantitative pond(21)And quantitative pond(21)The run-off of connection(22)And connecting tube(23);
The connecting tube(23)One end and quantitative pond(21)Connection, the other end and blood plasma pond(12)Connection.
2. a kind of centrifugal plasma according to claim 1 detaches CD, it is characterised in that:The whole blood pond(11)Before
Side and trailing flank are made of a plurality of curve;The center of curvature of any one curve is located on the axis of CD.
3. a kind of centrifugal plasma according to claim 2 detaches CD, it is characterised in that:The blood plasma pond(12)Before
Side and trailing flank are made of a plurality of curve;The center of curvature of any one curve is located on the axis of CD.
4. a kind of centrifugal plasma according to claim 1 detaches CD, it is characterised in that:The conduit(13)Axis
The center of curvature be located on the axis of CD.
5. a kind of centrifugal plasma according to claim 4 detaches CD, it is characterised in that:Vertically with the direction of CD
On, there are one to pass through conduit(13)The curved surface of axis;The curved surface is by whole blood pond(11)It is divided into top half and lower half portion;Institute
It is close to the part of CD axis to state top half;The top half and the volume ratio of lower half portion are 3:2.
6. a kind of centrifugal plasma according to claim 1 detaches CD, it is characterised in that:The run-off(22)It is located at
Quantitative pond(21)Close to the side of cd centre.
7. a kind of centrifugal plasma according to claim 1 detaches CD, it is characterised in that:The quantitative pond(21)It surrounds
The axis circular array of CD;The quantitative pond(21)It is sequentially connected in series;The run-off(22)Only corresponding quantitative pond
(21)Connection.
8. a kind of centrifugal plasma according to claim 7 detaches CD, it is characterised in that:The separator further includes
Diversion pipe(3);The diversion pipe(3)Both ends respectively with adjacent quantitative pond(21)Connection;The diversion pipe(3)Curvature in
The heart is located on the axis of CD.
9. a kind of centrifugal plasma according to claim 7 detaches CD, it is characterised in that:The quantitative collection device is also
Including aerial drainage pond(4);
The aerial drainage pond(4)With the last one run-off(22)Connection;
The connecting tube(23)One end and first blood plasma pond(12)Connection.
10. a kind of centrifugal plasma according to claim 9 detaches CD, it is characterised in that:The aerial drainage pond(4)With light
The minimum range of disk center is more than run-off(22)With the minimum range of cd centre.
Priority Applications (1)
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CN201810450960.6A CN108469367A (en) | 2018-05-11 | 2018-05-11 | A kind of centrifugal plasma separation CD |
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CN201810450960.6A CN108469367A (en) | 2018-05-11 | 2018-05-11 | A kind of centrifugal plasma separation CD |
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Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5061381A (en) * | 1990-06-04 | 1991-10-29 | Abaxis, Inc. | Apparatus and method for separating cells from biological fluids |
WO1991018656A1 (en) * | 1990-06-04 | 1991-12-12 | Abaxis, Inc. | Analytical rotors and methods for analysis of biological fluids |
US5591643A (en) * | 1993-09-01 | 1997-01-07 | Abaxis, Inc. | Simplified inlet channels |
WO2006038682A1 (en) * | 2004-10-01 | 2006-04-13 | Kabushiki Kaisya Advance | Solid-liquid separation/measuring structure and method of solid-liquid separation/measuring |
US20060263247A1 (en) * | 2005-01-28 | 2006-11-23 | Matsushita Electric Industrial Co., Ltd. | Blood processing apparatus and blood introducing method |
CN102239005A (en) * | 2009-11-03 | 2011-11-09 | 天津微纳芯科技有限公司 | Integrated detection chip and application method thereof |
US20120295781A1 (en) * | 2011-04-18 | 2012-11-22 | The Regents Of The University Of California | Microfluidic device for whole blood sample preparation |
CN105353159A (en) * | 2014-08-20 | 2016-02-24 | 余波 | Microfluidic test device and method for operating the same |
CN208270302U (en) * | 2018-05-11 | 2018-12-21 | 石家庄禾柏生物技术股份有限公司 | A kind of centrifugal plasma separation CD |
-
2018
- 2018-05-11 CN CN201810450960.6A patent/CN108469367A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5061381A (en) * | 1990-06-04 | 1991-10-29 | Abaxis, Inc. | Apparatus and method for separating cells from biological fluids |
WO1991018656A1 (en) * | 1990-06-04 | 1991-12-12 | Abaxis, Inc. | Analytical rotors and methods for analysis of biological fluids |
US5591643A (en) * | 1993-09-01 | 1997-01-07 | Abaxis, Inc. | Simplified inlet channels |
WO2006038682A1 (en) * | 2004-10-01 | 2006-04-13 | Kabushiki Kaisya Advance | Solid-liquid separation/measuring structure and method of solid-liquid separation/measuring |
US20060263247A1 (en) * | 2005-01-28 | 2006-11-23 | Matsushita Electric Industrial Co., Ltd. | Blood processing apparatus and blood introducing method |
CN102239005A (en) * | 2009-11-03 | 2011-11-09 | 天津微纳芯科技有限公司 | Integrated detection chip and application method thereof |
US20120295781A1 (en) * | 2011-04-18 | 2012-11-22 | The Regents Of The University Of California | Microfluidic device for whole blood sample preparation |
CN105353159A (en) * | 2014-08-20 | 2016-02-24 | 余波 | Microfluidic test device and method for operating the same |
CN208270302U (en) * | 2018-05-11 | 2018-12-21 | 石家庄禾柏生物技术股份有限公司 | A kind of centrifugal plasma separation CD |
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