CN108450320A - Breeding method of sterile line and maintainer line of non-branched rape - Google Patents
Breeding method of sterile line and maintainer line of non-branched rape Download PDFInfo
- Publication number
- CN108450320A CN108450320A CN201810053748.6A CN201810053748A CN108450320A CN 108450320 A CN108450320 A CN 108450320A CN 201810053748 A CN201810053748 A CN 201810053748A CN 108450320 A CN108450320 A CN 108450320A
- Authority
- CN
- China
- Prior art keywords
- generation
- sterile
- rape
- plant
- pedigree
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000009395 breeding Methods 0.000 title claims abstract description 13
- 230000001488 breeding effect Effects 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 11
- DPUOLQHDNGRHBS-UHFFFAOYSA-N Brassidinsaeure Natural products CCCCCCCCC=CCCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-UHFFFAOYSA-N 0.000 claims abstract description 10
- URXZXNYJPAJJOQ-UHFFFAOYSA-N Erucic acid Natural products CCCCCCC=CCCCCCCCCCCCC(O)=O URXZXNYJPAJJOQ-UHFFFAOYSA-N 0.000 claims abstract description 10
- DPUOLQHDNGRHBS-KTKRTIGZSA-N erucic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-KTKRTIGZSA-N 0.000 claims abstract description 10
- 230000036512 infertility Effects 0.000 claims abstract description 8
- 239000005864 Sulphur Substances 0.000 claims description 9
- 238000003306 harvesting Methods 0.000 claims description 9
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 6
- 208000000509 infertility Diseases 0.000 claims description 6
- 229930182470 glycoside Natural products 0.000 claims description 4
- 231100000535 infertility Toxicity 0.000 claims description 4
- -1 sulphur glycosides Chemical class 0.000 claims description 3
- 230000014759 maintenance of location Effects 0.000 abstract 2
- 230000000644 propagated effect Effects 0.000 abstract 2
- 238000001228 spectrum Methods 0.000 abstract 2
- 125000004383 glucosinolate group Chemical group 0.000 abstract 1
- 238000012423 maintenance Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 37
- 238000009331 sowing Methods 0.000 description 6
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 230000001086 cytosolic effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 240000002791 Brassica napus Species 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005070 ripening Effects 0.000 description 2
- 241000707825 Argyrosomus regius Species 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 241001164374 Calyx Species 0.000 description 1
- 241001674939 Caulanthus Species 0.000 description 1
- 241001573881 Corolla Species 0.000 description 1
- 241000286862 Siliqua Species 0.000 description 1
- 240000008866 Ziziphus nummularia Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for breeding sterile line and maintainer line of non-branched rape. The method comprises the following steps: sterile plants are found in the non-branched rape in the first year, and the homologous fertile plants are maintained in pairs to form a first generation sterile and maintenance line; and (3) breeding the harvested seeds in generation addition: selecting 5 sterile plants and 5 fertile plants to maintain backcross in pairs, and performing generation-adding propagation on the harvested seeds to form a backcross second generation pedigree; in the second year, selecting one line spectrum from 5 pairwise maintained sterile lines, and carrying out fertile and sterile backcross to maintain 5 lines to form a third generation line spectrum; the harvested third generation seeds are propagated by the same method, and the harvested seeds are propagated by generations to form a backcross fourth generation pedigree; in the third year, the sterility in the fourth generation pedigree and the retention parent are used for backcross and retention, and the harvested seeds are added with generations for propagation to form a backcross five-generation pedigree; in the fourth year, the sterility in the fifth generation of the genealogy is used for maintaining the backcross of the parent to form a backcross six generation of genealogy; the six-generation genealogical sterile character is basically stable, the sterile plant rate is 100%, and the erucic acid, glucosinolate and oil content can reach the breeding goal.
Description
Technical field
The present invention relates to agricultural plantation technology field more particularly to a kind of selection and breeding of no branch rape sterile line and maintainer
Method.
Background technology
Rape flower plant grows thickly straight, and stem is green to spend Huang, and in stacked growth is revolved, stem leaf, usually alternate do not have basal leaf
Stipule.Flower both sexes, radiation symmetric, 4 pieces of petal is in cross-shaped arrangement, and flower piece matter such as rice paper is light yellow meagre.Usual 6 pieces of stamen, 4
Long 2 is short, and commonly referred to as " tetradynamous stamens ", gynoecium is made of two carpels, and ovary position is top.Stem is cylindrical, and diameter exists mostly
Between 0.1-0.8 centimetres, multi-branched, cane is softer；Leaf alternate, Ye great, strong green, no petiole and support.Three, between April, the stem tip
Blossom, flower is raceme, and calyx piece four, yellow green, corolla pintongs, yellow is in cross.Fruit is siliqua, to the summer
In season, crack the seed that sheds when ripe, and atropurpureus also has yellow.
The rape of whole world cultivation is typically all that plant is tall and big, and plant height is in 170-220cm.Branch is more, primary effective branch
Number 8-12,25-30 days florescence, single plant silique number 250-400, mass of 1000 kernel 3.2-3.8g are easy lodging, Grain Ripening degree
It is inconsistent, it is unfavorable for Mechanization sowing and harvest.
Invention content
In view of this, an embodiment of the present invention provides a kind of selection of no branch rape sterile line and maintainer, it is main
Syllabus is selection and breeding without branch rape sterile line and maintainer for use in the parent without branch cross rape is prepared.
In order to achieve the above objectives, invention broadly provides following technical solutions：
On the one hand, an embodiment of the present invention provides a kind of selections of no branch rape sterile line and maintainer, including
Following steps：First Year is chosen sterile plant in no branch rape, is kept in pairs with fertile plant in homology, forms the first generation not
It educates strain and keeps strain；The seed of harvest is sowed in Qinghai Province's Ledu County adding generation, method is：Choose 5 plants of infertility
Strain and 5 plants of fertile plant pairing backcrossings are kept, and the seed kind harvested forms second backcross generation pedigree in Xixiang rape base；
Second year selects a pedigree and continues to be kept with fertile plant and sterile plant backcrossing in the strain that 5 keep in pairs
5 strains form third generation pedigree；By the third generation seed of harvest, continued in Qinghai Province's Ledu County with above-mentioned identical method
Add generation, the seed kind harvested forms four generation pedigrees of backcrossing in Xixiang rape base；
Third year continue to be returned holding with parent is kept with the sterile plant in forth generation pedigree, the seed kind harvested exists
Xixiang rape base forms five generation pedigrees of backcrossing；
4th year, continues to be returned holding with parent is kept with the sterile plant in the 5th generation pedigree, form six generation pedigrees of backcrossing；
Six generation pedigree infertility characters are basicly stable, and sterile plant rate 100%, erucic acid, sulphur glycosides, oil content reach expected breeding mesh
Mark.
Preferably, the plant height 80-120cm of the sterile line without branch rape, 10 or so day of florescence, single plant silique
Number 30-40, per seed number per pod 20~22, mass of 1000 kernel 3.5-4.9g, content of erucic acid 0.23~0.88%, sulphur resources 21.39
~24.27 μm of ol/g, oil content 46~55%.
Compared with prior art, the beneficial effects of the invention are as follows：
The method of the present invention is by finding and selection and breeding are without branch rape sterile line and maintainer, once it is bred as sterile line, it can be big
Testcross and screening restorer is measured, if be bred as without three system of branch rape cytoplasm, has also just been bred as without branch cross rape.It is this
Also without branch, only main inflorescence knot silique when cross rape maturation, plant is short, and florescence is short, and Grain Ripening degree is consistent, kind
Density in planting is not easy to lodge up to ten thousand plants/acre of 10-15 or more, and the florescence shortens 15-20 days than ordinary cole, harvest time shortening 7-10
It, vacates rear stubble rice 7 days or more growth periods, and solid foundation has been established for rice high yield.Meanwhile no branch rape is miscellaneous
It hands over kind that can broadcast sowing and mechanical planting, per mu yield estimation can reach 250kg or more, completely achievable Mechanization sowing and harvest.
Description of the drawings
Fig. 1 is the flow chart of no branch rape new material selection provided in an embodiment of the present invention.
Specific implementation mode
For further illustrate the present invention to reach the technological means and effect that predetermined goal of the invention is taken, below with compared with
Specific implementation mode, technical solution, feature and its effect applied according to the present invention is described in detail as after in good embodiment.Under
State it is bright in multiple embodiments in special characteristic, structure or feature can be combined by any suitable form.
Embodiment 1
Rape florescence in 2014 is numbering in Shaanxi Province Xixiang County jujube garden village Xibei Univ. of Agricultural ＆ Forest Science ＆ Technology's rape experimental plot
The Wild cabbage type for being 790 without in branch rape, finding there are 10 plants of sterile plants, for the year use together this 10 plants of sterile plants by 40 plants of fertile plants
Fertile plant is kept in system, as shown in Figure 1, to 10 pairs of materials of holding, harvested seed carries out adding generation, oil in Qinghai Province's Ledu County
Dish florescence only has two teams to show complete sterility, keeps strain to keep in pairs two complete sterility material selections, 5 sterile plants and 5,
Add the seed kind withheld and obtained in Xixiang rape base；
Final election in 2015 goes out a complete sterile line and 5 strains, harvested seed is kept to continue in Qinghai Province's Ledu County in pairs
It carries out adding generation, the seed withheld and obtained is added to continue autumn sowing kind in Xixiang rape base；
2016, in 5 strains, final election went out a complete sterile line and 5 strains, autumn sowing is kept to continue to be planted in west in pairs
Township rape base；
Continue within 2017 to keep complete sterile line in pairs 5 strains, final final election goes out two strains, and sterile character is substantially steady
Fixed, sterile plant rate 100%, erucic acid, sulphur glycosides, oil content reach expected breeding objective.It is bred as 796A and 796B, 889A and 889B two
A no branch rape cytoplasmic male sterile line and its corresponding maintainer；To six generations, sterile character has been completed at present
It is basicly stable；Wherein 796A and 796B plant heights 94cm, single plant silique 38 or so, mass of 1000 kernel 5.8g；Content of erucic acid 0.36%, sulphur
21.26 μm of ol/g of glycosides content, oil content reach 54.23%；Maturity period ratio has branch rape 5 days precocious；889A and 889B plant heights
79cm, single plant silique 31 or so, mass of 1000 kernel 4.8g；Content of erucic acid 0.46%, 25.26 μm of ol/g of sulphur resources, oil content reach
To 49.96%；Maturity period ratio has branch rape 7 days precocious.
Two novel no branch rape sterile lines have heredity in selection of the present invention, from backcrossing 2 generations to the 6th generation,
No branch and sterile character are stablized, and illustrate, with hereditability, to can be used for tissue culture without branch cross rape.
Selection of the present invention be bred as without branch rape cytoplasmic male sterile line and its corresponding maintainer, wherein
796A and 796B plant height 94cm, single plant silique 38 or so, mass of 1000 kernel 5.8g；Content of erucic acid 0.36%, 21.26 μ of sulphur resources
Mol/g, oil content reach 54.23%.Maturity period ratio has branch rape 5 days precocious；889A and 889B plant height 79cm, single plant silique
31 or so, mass of 1000 kernel 4.8g.Content of erucic acid 0.46%, 25.26 μm of ol/g of sulphur resources, oil content reach 49.96%.It is ripe
Phase ratio has branch rape 7 days precocious；It is the new germ plasm that current cabbage type rape cytoplasmic male sterile line has innovative value.
Selection of the present invention be bred as without branch rape cytoplasmic male sterile line, mass of 1000 kernel is big, and oil content is high, is group
Train the essential parent of cenospecies, and most most important parent material.If restorer can be selected, and then improved crossing
Kind, with the revolutionary meaning of rapeseed breeding, direction and the method for kind of rapeseed breeding can be led.Rape variety is had by original
Branch becomes no branch, and planting density increases to 150,000 plants or more by more than 10,000 plants per acre, and is not easy to lodge, and the florescence compares conventional oil
Dish shortens 20 days or more, and harvest time shortens 7-10 days, can broadcast sowing and planting with machinery, can also postpone to sow, for lower stubble rice high yield
Breed breeding vacates the growth period of 7-10.Finally make rape to the same mechanical planting of wheat, to the same mechanical harvest of wheat.
Place, those skilled in the art can not select from the prior art to the greatest extent in the embodiment of the present invention.
Disclosed above is only the specific implementation mode of the present invention, but scope of protection of the present invention is not limited thereto, is appointed
What those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, answer
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be with above-mentioned scope of the claims
It is accurate.
Claims (2)
1. a kind of selection of no branch rape sterile line and maintainer, which is characterized in that the described method comprises the following steps：
First Year is chosen sterile plant in no branch rape, is kept in pairs with fertile plant in homology, and first generation infertility strain is formed
With holding strain；The seed of harvest is sowed in Qinghai Province's Ledu County adding generation, method is：Choose 5 plants of sterile plants and 5 plants
Fertile plant pairing backcrossing is kept, and the seed kind harvested forms second backcross generation pedigree in Xixiang rape base；
Second year selects a pedigree and continues to be kept for 5 with fertile plant and sterile plant backcrossing in the strain that 5 keep in pairs
Strain forms third generation pedigree；By the third generation seed of harvest, continue to add in Qinghai Province's Ledu County with above-mentioned identical method
Generation, the seed kind harvested form four generation pedigrees of backcrossing in Xixiang rape base；
Third year continue to be returned holding with parent is kept with the sterile plant in forth generation pedigree, the seed kind harvested is in Xixiang
Rape base forms five generation pedigrees of backcrossing；
4th year, continues to be returned holding with parent is kept with the sterile plant in the 5th generation pedigree, form six generation pedigrees of backcrossing；
Six generation pedigree infertility characters are basicly stable, and sterile plant rate 100%, erucic acid, sulphur glycosides, oil content reach expected breeding objective.
2. a kind of selection of no branch rape sterile line and maintainer as described in claim 1, which is characterized in that described
The plant height 80-120cm of the sterile line without branch rape, 10 or so day of florescence, single plant silique number 30-40, the grain per angle
Number 20~22, mass of 1000 kernel 3.5-4.9g, content of erucic acid 0.23~0.88%, 21.39~24.27 μm of ol/g of sulphur resources contain
Oil mass 46~55%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810053748.6A CN108450320A (en) | 2018-01-19 | 2018-01-19 | Breeding method of sterile line and maintainer line of non-branched rape |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810053748.6A CN108450320A (en) | 2018-01-19 | 2018-01-19 | Breeding method of sterile line and maintainer line of non-branched rape |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108450320A true CN108450320A (en) | 2018-08-28 |
Family
ID=63221012
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810053748.6A Pending CN108450320A (en) | 2018-01-19 | 2018-01-19 | Breeding method of sterile line and maintainer line of non-branched rape |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108450320A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1220601A1 (en) * | 1999-09-28 | 2002-07-10 | University of Delhi, South Campus | Stable cytoplasmic male sterile brassica campestris plant which contain "polima" cytoplasm and method for obtaining such plants |
| EP2220930A2 (en) * | 2007-06-13 | 2010-08-25 | Syngenta Participations AG | New hybrid system for brassica napus |
| CN102150613A (en) * | 2011-04-20 | 2011-08-17 | 西南大学 | Method for breeding cytoplasm male sterile line of novel cabbage type rape |
| CN106234207A (en) * | 2016-07-30 | 2016-12-21 | 南京市蔬菜科学研究所 | A kind of method of Chinese cabbage cytoplasmic male sterile line selection-breeding |
| CN107148906A (en) * | 2017-04-19 | 2017-09-12 | 西北农林科技大学 | A kind of selection without branch rape new material |
-
2018
- 2018-01-19 CN CN201810053748.6A patent/CN108450320A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1220601A1 (en) * | 1999-09-28 | 2002-07-10 | University of Delhi, South Campus | Stable cytoplasmic male sterile brassica campestris plant which contain "polima" cytoplasm and method for obtaining such plants |
| EP2220930A2 (en) * | 2007-06-13 | 2010-08-25 | Syngenta Participations AG | New hybrid system for brassica napus |
| CN102150613A (en) * | 2011-04-20 | 2011-08-17 | 西南大学 | Method for breeding cytoplasm male sterile line of novel cabbage type rape |
| CN106234207A (en) * | 2016-07-30 | 2016-12-21 | 南京市蔬菜科学研究所 | A kind of method of Chinese cabbage cytoplasmic male sterile line selection-breeding |
| CN107148906A (en) * | 2017-04-19 | 2017-09-12 | 西北农林科技大学 | A kind of selection without branch rape new material |
Non-Patent Citations (2)
| Title |
|---|
| JIA GUO等: "Transcriptome sequencing and de novo analysis", 《MOLECULAR BREEDING》 * |
| 袁卫红等: "甘蓝型油菜细胞质雄性不育系3A、保持系3B的选育", 《湖北农业科学》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Donald et al. | The convergent evolution of annual seed crops in agriculture | |
| CN110100723B (en) | Hybridization breeding method of fast-period cabbage type rape and application thereof | |
| CN108925423B (en) | A kind of heat-resisting germplasm selection of Rise's boot period | |
| KR20040108792A (en) | Tetraploid watermelons producing small fruits | |
| CN101743902B (en) | Construction of Brassica napus recessive nuclear sterile near-isogenic line and single cross hybrid production technology | |
| CN114223533A (en) | Breeding method of high-yield and easy-to-peel tartary buckwheat variety | |
| JP7350767B2 (en) | Chia variety called Lemborg | |
| Draper | Tetraploid hybrids from crosses of diploid, tetraploid, and hexaploid Vaccinium species | |
| CN105918109B (en) | A kind of fast dish selection of purple | |
| CN108450320A (en) | Breeding method of sterile line and maintainer line of non-branched rape | |
| CN106962186A (en) | A kind of method of sweet tea buckwheat variety rejuvenation resistant to lodging | |
| CN112841019A (en) | Method for breeding new species of flowering Chinese cabbage | |
| Mosenda et al. | Assessment of agronomic traits of selected spider plant (Cleome gynandra L.) accessions | |
| CN105850718A (en) | Method for prolonging rapeseed flowering period and preparation method of sterile rapeseed | |
| US20200329656A1 (en) | Scabiosa atropurpurea plants and methods of producing same | |
| Freyre et al. | Artificial hybridizations in five species of Chilean Nolana (Solanaceae) | |
| KR102342057B1 (en) | Cold Stress and Clubroot Disease Resistant Novel Chinese Cabbage Variety | |
| Tamilselvi et al. | DEVELOPMENT OF INTERSPECIFIC HYBRID ROOTSTOCKS USING CUCURBITAMOSCHATA DUCH EX. POIR AND CUCURBITA MAXIMA LINES. | |
| CN115191252B (en) | Multi-male parent single female parent hybridization method for soybean | |
| JP6973841B1 (en) | Rice seeds and mixed rice | |
| CN112438199B (en) | Breeding method of rape variety suitable for balcony cultivation | |
| CN111528025B (en) | Method for shortening breeding years of potatoes | |
| Dutta et al. | High yielding mulberry production through controlled pollination for enhanced vegetative growth and early sprouting suitable for tropical agroclimatic regions | |
| Kisku et al. | Advances in Litchi biotechnology: achievements and opportunities | |
| Chakraborty | Assessing the cropping and weediness potential of two potentially new oilseed species in southern Ontario: Euphorbia lagascae and Centrapalus pauciflorus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180828 |
|
| WD01 | Invention patent application deemed withdrawn after publication |