JP6973841B1 - Rice seeds and mixed rice - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a rice seed having a large rice grain and improved lodging resistance, and mixed rice using polished rice obtained from the fruit of the rice seed. SOLUTION: The rice seed is a rice seed characterized by being a rice seed specified by accession number FERM BP-22401 or a progeny line. The mixed rice is the first polished rice obtained by milling the first polished brown rice obtained from the rice species specified by the accession number FERM BP-22401 or the rice species of the progeny line, and the first polished rice. A second polished rice obtained by milling a second polished rice obtained from a rice species different from the first polished rice, and a first polished rice mixed in a desired ratio with respect to the number of rice. And a packaging container for storing the second polished rice. [Selection diagram] Fig. 1

Description

The present invention relates to a rice seed having a large rice grain and improved lodging resistance, and mixed rice using polished rice obtained from the fruit of this rice seed.

The world population is estimated to be about 7.7 billion in 2019, and it is increasing year by year, mainly in developing countries, and it is said that about 10% of the world's people are suffering from hunger. Food self-sufficiency has become a global issue, with the prospect of reaching about 10 billion in 2050.

Cereals make up a large proportion of food, many of which are relatively easy to cultivate, are well preserved, and contain nutrients that are essential for nutrition, so they play an important role. Among them, rice (rice seed Oryza sativa) is a cereal that is a staple food in many countries because it is easy to store and cook, has a light taste and does not get tired of it. Increasing rice yields is essential to solving global food problems.

For example, "Akitakomachi", one of the main varieties of rice in Japan, is uruchi rice (japonica rice) developed by Akita prefecture and cultivated nationwide for many years, mainly in the Tohoku region. According to the "Trends in Cultivation of Paddy Rice Produced in 2018 by Variety" published by the Public Interest Incorporated Association Rice Stable Supply Securing Support Organization on April 11, 2019, the cropping ratio of Akitakomachi in 2018 was 6.8%, nationwide. It was the 4th place. The taste of Akitakomachi is as high as that of Sasanishiki and Koshihikari, and it has a strong stickiness. However, according to Appendix 16 of Non-Patent Document 1, the lodging resistance of Akitakomachi is evaluated to be "slightly weak" among the rice species in Japan. It is known that when rice falls down, harvesting work becomes difficult and the yield and quality deteriorate. When the fallen ears are submerged in the water of the paddy field, the paddy may germinate and it may not be possible to harvest.

According to Appended Tables 9 to 11 of Non-Patent Document 1, Akitakomachi brown rice is evaluated to have a "medium" size (fifth on a nine-point scale) similar to other Japanese glutinous rice. In order to increase the yield in Akitakomachi, it is possible to improve the yield per plant by increasing the size of rice grains and the number of grains, but in Akitakomachi with weak lodging resistance, it is not possible to put a further load on the plant. There is a barrier that is not desirable.

Registered Japanese varieties Rice species screening standards (March 2018 version)

The present invention has been made by paying attention to the above-mentioned problems, and provides a rice seed having a large rice grain and improved lodging resistance, and mixed rice using polished rice obtained from the fruit of this rice seed. The purpose is to do.

In order to achieve the above object, the first aspect of the present invention is a rice species identified by accession number FERM BP-22401 (Oryza sativa) or a successor strain.

The second aspect of the present invention was obtained by (a) milling the first polished brown rice obtained from the rice species (Oryza sativa) specified by accession number FERM BP-22401 or the rice species which is a progeny line. The first polished rice and (b) the second polished brown rice obtained from a rice seed of a different variety from the first polished rice, which is mixed at a desired ratio with respect to the number of the first polished rice, is polished. The gist is that it is a mixed rice characterized by comprising (c) a packaging container for storing the first polished rice and the second polished rice.

According to the present invention, it is possible to provide a rice seed having a large rice grain and improved lodging resistance, and mixed rice using polished rice obtained from the fruit of this rice seed.

It is a drawing substitute photograph which shows the appearance of the grain of the refined brown rice of the rice type and the refined brown rice of the comparative variety Akitakomachi which concerns on embodiment of this invention. It is a schematic diagram of the appearance at the time of harvesting of a general rice species (paddy rice). 3 (a) is an enlarged view of a portion A of FIG. 2, and FIG. 3 (b) is a diagram showing a state in which the second leaf 19 is removed from the state of FIG. 3 (a). FIG. 4 (a) is an enlarged view of the tip portion of a general rice seed immediately before heading, and FIG. 4 (b) is a diagram showing a state in which the young ear 23 has headed from the state of FIG. 4 (a). .. It is a table which shows the comparison of the amino acid analysis result of the refined brown rice of the rice type which concerns on Example 1, and the refined brown rice of the comparative variety Akitakomachi. It is a table summarizing the trait comparison of the rice species and the comparative cultivar Akitakomachi according to Example 1. It is a table summarizing the taste comparison of the rice varieties and the comparative varieties Akitakomachi according to Example 1.

Hereinafter, embodiments of the present invention will be described with reference to the drawings. In the description of the drawings below, the same or similar parts are designated by the same or similar reference numerals. However, it should be noted that the drawings are schematic. Therefore, the specific breeding method, etc. should be judged more diversely in consideration of the purpose of the technical idea that can be understood from the following explanation.

Further, the embodiments of the present invention shown below exemplify an apparatus, a substance, and a method for embodying the technical idea of the present invention, and the technical idea of the present invention is a material of the apparatus and the substance. , Shape, structure, arrangement, etc. are not specified to the following. The technical idea of the present invention is not limited to the contents described in the embodiments of the present invention, and various changes are made within the technical scope specified by the organic binding of the matters specifying the invention described in the claims. Can be added.

The rice species according to the embodiment of the present invention is a rice species of paddy rice discovered in a paddy field of Akitakomachi, which is a comparative variety, and obtained through isolation breeding of mutants, self-breeding, and the like. That is, among the strains after the heading stage, at least one mutant strain that is relatively tall, has white awns (nogi, noge, or bob) and has a large number of grains is isolated from the mutant strain. Obtain the obtained next-generation seeds. In the present specification, seeds obtained from mutant strains and strains grown from the seeds are defined as "F1 generation", and seeds obtained from F1 generation strains and strains grown from the seeds are defined as "F2". Defined as "generation". The F2 generation and subsequent generations are similarly defined as "F3 generation", "F4 generation", and so on. Further, in the present specification, the "seed" of a rice seed means "seed paddy", and unless otherwise specified, it means a state with paddy. In general, "seed paddy" is also simply referred to as "paddy", and therefore, "paddy" and "seed paddy" are used interchangeably in the present specification. Further, the term "fruit" of a rice species in the present specification means "seed", that is, "seed", that is, "fruit" in accordance with the meaning of "a part where a pollinated pistil ovary has developed and its appendages", which is a biological "fruit". It shall be used in the same meaning as "seed paddy" or "paddy".

In the F1 generation, it is bred by self-breeding in the same way as the breeding of the comparative variety Akitakomachi. Most of the awns of F1 generation stocks are red. In the next generation of F2 generation, they are bred by either self-breeding or mating the F2 strain as a mother strain and the Akitakomachi strain as a father strain. Many of the F2 generations have red awns, but only the strains with white awns are selected to obtain seeds for the next generation. The F3 generation is cultivated from the obtained seeds, and only the strains with white awns are selected as in the F2 generation to obtain seeds to be used for the next generation. The F4 generation is bred from the obtained seeds, and only the strains with white awns are selected as in the case of the F2 generation and the F3 generation. A group of strains with uniform traits is selected to obtain seeds to be used for the next generation. After the F5 generation, strains with white or no awns are selected, and among them, the culm thickness and hardness are the same as those of the previous generation, and the height is the same as that of the previous generation. A group of strains having the above is selected to obtain seeds to be used for the next generation. It is cultivated for fixation up to at least the F6 generation, and the rice seeds according to the embodiment are obtained.

"Awn" refers to the needle-shaped hair at the tip of the paddy, but in the Akitakomachi strain of the comparative variety, awns can hardly be confirmed on the paddy. Awns tend to be found in paddy in wild grain strains. Strains with awns that are rarely found in cultivars are usually removed as soon as they are found, as they can adversely affect the maintenance of the desired traits of the cultivar. The length of the paddy awns of the mutant strain from which the seeds of the F1 generation can be obtained is about 1 to 3 cm, gradually shortens in the strains after the F2 generation, and becomes about 0.1 to 0.5 cm after the F4 generation. In addition, in the mutant strains from which seeds of the F1 generation can be obtained, awns are confirmed in most of the paddy, but after the F5 generation, the majority of the strains have no awns.

As used herein, the term "strain" of a rice species refers to an individual grown from a single seed. Although it depends on the cultivation method, several rice seeds are often planted together as one "seedling" in one place. Therefore, in that case, the smallest unit of rice planting is "seedling", and "seedling" is a collection of "strains" grown from several seeds. Furthermore, in the growing process, "tillering" occurs near the root of the "strain" and the number of stems increases. Whether or not each stem produces spikes depends on nutritional conditions, etc. Generally, stems with spikes are called effective stems, and stems without spikes are called invalid stems.

As used herein, the "back" of a rice seed strain means from the root to the tip of the top of the strain. Depending on the growing season, the root of the plant to the tip of the uppermost part may naturally stand upright in the vertical direction, but as shown in Fig. 2, the leaves or ears that become the tip part are under their own weight after the heading stage. Often hangs down. After the heading stage, the leaf or ear, which is the tip, is lifted and the height of the rice seed strain is measured so that it is in a straight line from the root to the tip. For example, in FIG. 2, the spike 2, the flag leaf 11 or the fifth leaf 13 of the strain 1 is lifted so as to be in a straight line in the vertical direction from the root of the strain, and the height of the strain is measured with the tallest one. ..

As for the breeding method in the F1 generation, normal open-field cultivation can be adopted, but house cultivation may also be used. As the pollination method, a normal self-pollination method such as an air medium can be adopted. Rice seeds are plants that are easily self-pollinated due to their structure, but in the natural world, they are partially cross-pollinated.

As for the breeding method in the F2 generation, a self-breeding method by self-pollination may be used, or a strain of Akitakomachi, which is a comparative variety, may be bred as a father strain. For that purpose, in open-field cultivation, which is a natural mating method, it is preferable to plant the F2 generation strain and the comparative variety Akitakomachi strain in close proximity. For example, it is a method of planting a row of F2 generation seedlings and a row of comparative varieties of Akitakomachi seedlings at a distance of about 25 to 35 cm. It is preferable to plant the F2 generation about one week earlier in order to align the flowering period of the F2 generation with that of Akitakomachi. As for the mating method in the F2 generation, an artificial mating method in which pollen of the father strain is sprinkled on the pistil of the mother strain may be adopted. In both self-pollination and cross-pollination, only the strains with white paddy awns are selected from the strains grown in the F2 generation to obtain seeds of the F3 generation. The method for raising the F3 generation is the same as that for the F1 generation, and only the strains with white awns are selected to obtain the seeds of the F4 generation.

The method of raising the F4 generation is the same as that of the F2 generation and the F3 generation, and only the strains with white awns are selected, and among them, the traits of relatively thick and hard culms and short height are all present. A group of strains is selected to obtain seeds to be used for the next generation. The "culm" of a rice seed strain refers to a hollow structure inside the stem, which can be seen by removing the leaf sheath on the outside from the stem. As shown in FIG. 3A, the second leaf 19 has a leaf blade 31a separated from the stem and a leaf sheath 33a wrapping the stem. When the second leaf 19 is removed from the stem, as shown in FIG. 3 (b), the vicinity of the root of the third leaf 17, the node 35, and the culm 37 are exposed. The culm 37 is also present inside the leaf sheath 33b of the third leaf 17, and is a central structure that supports the inside of the stem from the root to the vicinity of the tip. Therefore, in rice seeds, the thickness of the culm is directly related to the thickness of the stem, and the fact that the culm is thick and hard is almost synonymous with the fact that the stem is thick and hard.

The training method for the F5 generation and beyond is the same as for the F4 generation. After the F5 generation, strains with white or no awns are selected, and among them, the culm thickness and hardness are the same as those of the previous generation, and the height is the same as that of the previous generation. A group of strains having the above is selected to obtain seeds to be used for the next generation. The seeds and strains of rice seeds according to the embodiment of the present invention are obtained by growing for fixing until the F6 generation or later.

The refined brown rice obtained from the fruit of the rice seed according to the embodiment of the present invention has a larger trait than the refined brown rice obtained from the fruit of the comparative variety Akitakomachi. That is, the refined brown rice obtained from the fruits of the rice seeds according to the embodiment (hereinafter referred to as "large grain brown rice") is higher than the refined brown rice obtained from the fruits of the comparative variety Akitakomachi (hereinafter referred to as "comparative brown rice"). It is long, wide, and heavy. In the present specification, "length of brown rice" indicates the length of refined brown rice, which is a trait evaluation item corresponding to trait number 58 in the characteristic table in the UPOV Convention, and is "short", "medium", or "long" depending on the degree of length. It is ranked as. Further, "width of brown rice" in the present specification indicates the width of refined brown rice, and is a trait evaluation item corresponding to trait number 59 in the characteristic table in the UPOV treaty, and is "narrow", "medium", and "wide" depending on the degree of width. It is ranked as. The grain of large brown rice according to the embodiment of the present invention can be evaluated as "long" when the length is about 6.2 to 6.8 mm, and can be evaluated as "wide" when the width is about 2.9 to 3.3 mm. On the other hand, the grains of comparative brown rice can be evaluated as "medium" when the length is about 5.2 to 5.8 mm, and can be evaluated as "medium" when the width is about 2.4 to 2.8 mm. In the present specification, "1000-grain weight of brown rice" indicates 1000-grain weight of refined brown rice, and is a trait evaluation item corresponding to trait number 58 in the characteristic table of rice species related to the application for registration of Japanese varieties, and is "small" or "medium" depending on the degree of weight. It is ranked as "Large". The 1000-grain weight of large brown rice indicating the yield of rice seeds according to the embodiment is about 31 to 34 g and can be evaluated as "large", and the 1000-grain weight of comparative brown rice indicating the yield of the comparative variety Akitakomachi is about 22 to 25 g and is "medium". Can be evaluated as. By milling the large-grain brown rice according to the embodiment into polished rice and storing the polished rice in a packaging container such as a rice bag, "single raw material rice" is labeled in accordance with the "brown rice and milled rice quality labeling standards". Can be sold. Since the "single raw material rice" according to the embodiment is a large grain of polished rice, the number of polished rice contained in the same capacity packaging container is smaller than the number of polished rice obtained from the comparative brown rice.

In the rice seeds according to the embodiment, about 2800 to 3200 grains of paddy can be obtained as fruits from one strain. When comparing the number of grains of Akitakomachi, which is a comparative variety in which about 1700 to 1800 grains of paddy are obtained as fruits from one strain, the yield is about 1.5 to 1.9 times.

The large-grain brown rice according to the embodiment of the present invention contains more amino acids related to umami, that is, aspartic acid and glutamic acid, than the comparative brown rice. The large-grain brown rice according to the embodiment has 1.3 times or more aspartic acid and 1.1 times or more glutamic acid as compared with the comparative brown rice. Therefore, from the rice seeds according to the embodiment, it is possible to obtain rice containing a larger amount of umami components than the comparative variety Akitakomachi.

The rice seeds according to the embodiment are clearly different from the comparative varieties Akitakomachi in terms of traits other than the appearance of brown rice. When the rice seeds according to the embodiment are planted at the same time as the comparative cultivar Akitakomachi, the heading period is several days earlier and the maturity period is several days later. The "heading period" in the present specification is the day when 50% of the effective number of stems of the strain is headed and is determined by observation, and is a trait evaluation item corresponding to trait number 19 in the characteristic table in the UPOV Convention. Is. As shown in FIGS. 4 (a) and 4 (b), the emergence of the young ear 23 from the flag leaf 11 at the most advanced portion of the strain is “heading”. The heading period of the rice seeds according to the embodiment can be evaluated as "quite early", and the heading period of the comparative variety Akitakomachi can be evaluated as "early". Further, the "maturity period" in the present specification is the day when most of the normal paddy is yellowed and is determined by observation, and the trait evaluation corresponding to the trait number 44 in the characteristic table in the UPOV Convention. It is an item. The maturity period of the rice seeds according to the embodiment can be evaluated as "slightly early", and the maturity period of the comparative variety Akitakomachi can be evaluated as "early". The early heading period means that the speed at which the spikes emerge, bloom, and reach maturity is fast, and the probability of avoiding various troubles in the immature period increases. Late maturity means that it takes longer to mature, which is a trait related to the large size of brown rice.

In addition, the rice seeds according to the embodiment have thicker culms than the comparative varieties Akitakomachi. The "culm thickness" in the present specification refers to the thickness of the culm between the lowest site nodes in the milk ripening stage, and is classified into "thin", "medium", and "thick" by observation. This "culm thickness" is also a trait evaluation item corresponding to trait number 25 in the trait table in the UPOV Convention. The rice seeds according to the embodiment can be evaluated as "thick", and the comparative variety Akitakomachi can be evaluated as "medium". The thick culm has the advantage that even if the size of brown rice is large and the load is applied to the ears as in the rice seeds according to the embodiment, it becomes stronger due to lodging. In fact, in the rice seeds according to the embodiment, the spikes are bent, but the lodging resistance is greatly improved as compared with the comparative variety Akitakomachi. The lodging resistance is a trait evaluation item corresponding to the trait number 71 in the characteristic table of rice species related to the application for registration of Japanese varieties, and is ranked into "weak", "medium", "strong" and the like by observation. The rice seeds according to the embodiment can be evaluated as "strong", and the comparative variety Akitakomachi can be evaluated as "slightly weak".

The large-grain brown rice according to the embodiment has a higher taste value than the comparative brown rice by the taste analysis device. Further, the protein content, which is considered to have an adverse effect on the taste when the amount is too large, is also lower in the large-grain brown rice according to the embodiment than in the comparative brown rice. Further, the fatty acid content, which is said to easily generate the odor of old rice when the amount is too large, is also lower in the large-grain brown rice according to the embodiment than in the comparative brown rice. The odor of old rice has an adverse effect on the taste, and the rice that produces the odor of old rice tends to be shunned by consumers.

The rice seeds according to the embodiment have cold resistance and heat resistance as compared with the comparative variety Akitakomachi. Akitakomachi is cultivated especially in the cold regions of the Tohoku region, and cold resistance is a problem, but the rice seeds according to the embodiment are very easy to cultivate. Regarding the cultivation of the rice seeds according to the embodiment, the same cultivation method as that of Akitakomachi, which is a comparative variety, may be used, or other methods may be used. In the same cultivation method as Akitakomachi, first, seeds are selected and disinfected, soaked and germinated. Next, the germinated seeds are sown and raised in a greenhouse. Rice is planted when the leaves are 3.0 to 3.5 years old, and fertilizers and herbicides are sprayed at the same time. During the subsequent growing, weeding and pesticide spraying are applied each time, and when it matures, rice is harvested and harvested. For the next year's breeding, the paddy obtained in the current year is dried, treated with awns, etc., and stored as seed paddy. Regarding the same cultivation method as this Akitakomachi, it is necessary to select a fertilizer, pesticide, etc. that are suitable for the variety and growing environment, but the outline is no different from the normal cultivation method of paddy rice.

The strains grown from the seeds (seed paddy) of the rice seeds according to the embodiment and the seeds obtained from the strains are also the strains and seeds having the same traits, characteristics, effects, etc. as those of the "rice seeds according to the embodiment". .. That is, it is naturally possible to paraphrase the progeny line by self-breeding of the rice seed according to the embodiment as the rice seed according to the embodiment. Since the rice seeds according to the embodiment are rice seeds that have been sufficiently cultivated for fixation at least up to the F6 generation, the progeny lines obtained by self-pollination after the F6 generation are the rice according to the embodiment. It has the same traits, characteristics, and effects as the species.

As an example of the rice seeds according to the embodiment, for example, the rice seeds deposited under the accession number FERM BP-22401 or the progeny line can be mentioned. The rice varieties deposited under the accession number FERM BP-22401 are the same varieties of rice varieties as the rice varieties according to Example 1 described later. The deposit information of the rice seed is as follows.
Accession number: FERM BP-22401
Identification display: ZS-001
Type of deposit: International deposit Institution name: National Institute of Technology and Evaluation Patent Biology Deposit Center Address of deposit institution: Room 2-5-8 Kazusakamatari, Kisarazu City, Chiba Prefecture, Japan 292-0818 Deposit date (Original deposit date): November 11, 2020

As for the large-grain brown rice according to the embodiment, the large-grain brown rice "first polished brown rice" is milled into "first polished rice", and the comparative brown rice "second polished brown rice" is milled into "second milled rice". When rice is cooked as "mixed rice" mixed in a certain ratio as "polished rice", it becomes more favorable in the sensory evaluation of the taste of white rice. Therefore, by mixing the first polished rice and the second polished rice in a desired ratio within the range of 1: 9 to 9: 1 in terms of grain number ratio and storing the rice in a packaging container such as a rice bag, "brown rice" is obtained. And can be sold with the label of "multiple raw rice" or "blended rice" in accordance with the "Polished Rice Quality Labeling Standards". The grain ratio in the range of 1: 9 to 9: 1 can be arbitrarily selected by the consumer's wishes. Bag materials such as cotton packs, craft packs, laminated packs, and poly packs can be used for the packaging container. The plurality of raw material rice or the blended rice composed of the mixed rice of the first polished rice and the second polished rice can provide the consumer with the mixed rice having a good taste. In particular, when "Akitakomachi" is adopted as the second polished rice, the grain ratio of the first polished rice to the second polished rice is 8: 2 from the comprehensive viewpoint of taste, texture, appearance, etc. ~ 6: 4 is more preferable. More preferably, it is about 7: 3. Since the mixed rice of the first polished rice and the second polished rice contains a large grain of the first polished rice, the total number of polished rice contained in the same capacity packaging container is 100% of the second polished rice. Less than the case. In addition, the polished rice of other varieties different from Akitakomachi is adopted as "the other second polished rice", and the first polished rice and the other second polished rice are within the range of 1: 9 to 9: 1. It may be mixed at a desired grain number ratio and sold with a label of a plurality of raw rice (blended rice). Even a mixed rice of the first polished rice and the other second polished rice can enjoy a good taste according to the consumer's wishes.

Since the rice seeds according to the embodiment have large seeds and high yield, refined brown rice and polished rice can also be used as feed rice or processed rice. The paddy yield of rice seeds according to the embodiment is 780 to 850 kg / 10a, and the calculated brown rice yield (80% of the paddy yield) is 624 to 680 kg / 10a. The brown rice yield of the rice seeds according to the embodiment is very high as compared with the brown rice yield of the comparative variety Akitakomachi of about 600 kg / 10a. Further, the large-grain brown rice according to the embodiment is suitable as sake rice because it has a size that makes it easy to polish rice in order to bring out the whiteness of the central part and has a protein content lower than that of the comparative variety Akitakomachi.

When the large-grain brown rice according to the embodiment is polished to be polished rice, it gives off a mellow and sweet odor more than the polished rice of the comparative variety Akitakomachi.

Hereinafter, the present invention will be specifically described with reference to examples, but the present invention is merely described for the purpose of illustration, and the present invention is not limited to these examples.
[Example 1]

The rice seeds according to Example 1 were obtained in the rice fields in the northern part of Akita Prefecture by the same method as the rice seeds according to the embodiment. In the paddy field of Akitakomachi, which is a comparative variety, among the strains after the heading stage, mutant strains that are relatively tall, have white awns, and have a large number of grains are selected and isolated, and the obtained seeds are referred to as F1 generation. bottom. In the F1 generation, they were bred by self-breeding in the same way as the comparative varieties of Akitakomachi. Most of the awns of F1 generation stocks were red. In the next generation F2 generation, the row of F2 generation seedlings and the row of comparative varieties of Akitakomachi seedlings are planted at a distance of about 25 to 35 cm, and the rice planting time is adjusted so that both flowering periods are aligned. Raised. In the F2 generation, many of them had red awns, but only the strains with white awns were selected to obtain seeds of the F3 generation. The F3 generation was cultivated from the obtained seeds, and only the strains having white awns were selected in the same manner as the F2 generation to obtain F4 generation seeds. The F4 generation is bred from the obtained seeds, and only the strains with white awns are selected as in the case of the F2 generation and the F3 generation. A group of strains having the same traits was selected to obtain seeds of the F5 generation. In the F5 generation, strains with white or no awns are selected, and among them, the culm thickness and hardness are the same as those of the F4 generation, and the height is the same as that of the F4 generation. The strains having were selected to obtain F6 generation seeds. In the F6 generation strains, the number of awned paddy itself decreased, and the proportion of white awns in the awned strains was 90% or more. In addition, among the F6 generation strains, the majority of the strains had the trait of thick and hard culm and short height. F7 generation seeds were obtained from F6 generation strains and grown by self-pollination in the same manner as before F6 to obtain F8 generation seeds. The obtained seeds of the F8 generation were cultivated to obtain the rice seeds according to Example 1. The method of growing from seeds of each generation was the same as the method of cultivating normal paddy rice (cultivation method of Akitakomachi) unless otherwise specified in the above. Various observations and analyzes were carried out on the rice seed strain (F8 generation) and the seeds (F9 generation) obtained from the strain according to Example 1 as follows.

The amino acid content of the large-grain brown rice and the comparative brown rice (Akitakomachi) according to Example 1 was analyzed. In the large-grain brown rice according to Example 1, the content of amino acids related to umami was higher than that in the comparative brown rice. That is, as shown in FIG. 5, aspartic acid, which is an umami component, was 770 mg in 100 g of the large-grain brown rice according to Example 1, and 590 mg in 100 g of the comparative brown rice. The amount of glutamic acid, which is also regarded as an umami component, was 1210 mg in 100 g of the large-grain brown rice according to Example 1, and 1030 mg in 100 g of the comparative brown rice. The comparison results of other amino acid contents are as shown in the table of FIG.

As shown in the photograph of FIG. 1 and the table of FIG. 6, the rice seeds according to Example 1 had a large grain trait of brown rice as compared with the comparative cultivar Akitakomachi. In FIG. 1, the large-grain brown rice according to Example 1 is the lower two grains, and the comparative brown rice is the upper two grains. The grains of large brown rice according to Example 1 were "long" with a length of about 6.5 mm and "wide" with a width of about 3.1 mm. On the other hand, the grains of comparative brown rice were "medium" with a length of about 5.5 mm and "medium" with a width of about 2.6 mm. The 1000-grain weight of the large-grain brown rice according to Example 1 was about 32.2 g, which was "large", and the 1000-grain weight of the comparative brown rice was about 23.3 g, which was "medium". The large-grain brown rice according to Example 1 was a seed of the F9 generation, but the seeds of the F7 generation and later had a common large-grain trait (thousand-kernel weight of brown rice: 31-34 g).

As shown in the table of FIG. 6, the rice seeds according to Example 1 had a heading period of 2 days earlier and a maturity period of 2 days later when planted at the same time as the comparative cultivar Akitakomachi. The heading period of the rice seeds according to Example 1 was "quite early" and the maturity period was "slightly early", and the heading period and maturity period of the comparative cultivar Akitakomachi were both "early". The rice seed strain according to Example 1 was of the F8 generation, but in common with the strains of the F6 generation and later, the heading period was "quite early" and the maturity period was "slightly early".

In addition, the rice seeds according to Example 1 have thicker culms than the comparative varieties Akitakomachi. The culm of the rice seed according to Example 1 was "thick", and the culm of the comparative variety Akitakomachi was "medium". Actually, as shown in FIG. 6, the ear of the rice seed according to Example 1 was bent, but the lodging resistance could be evaluated as "strong", which was greatly improved from the "slightly weak" of the comparative variety Akitakomachi. The rice seed strain according to Example 1 was of the F8 generation, but in the strains of the F6 generation and later, the culm was thick and the spikes were bent, but the lodging resistance was "strong".

The component content and taste value of the large-grain brown rice and the comparative brown rice according to Example 1 were analyzed. As shown in FIG. 7, as a taste analysis device, a rice grain taste meter manufactured by Satake Corporation (“Taste Meter” is a registered trademark of Satake Corporation), a taste analyzer manufactured by Shizuoka Machinery Co., Ltd., and Kett Science Institute Headquarters As a result of measurement using a component analyzer manufactured by Kett Science Institute Headquarters, the taste value of the large-grain brown rice according to Example 1 was higher than that of the comparative brown rice in all three types of taste analysis instruments. In addition, the protein content, which is considered to have an adverse effect on the taste if too much, was lower in the large-grain brown rice according to Example 1 than in the comparative brown rice. Further, regarding the fatty acid content, which is said to easily generate the odor of old rice when the amount is too large, the large-grain brown rice according to Example 1 is compared with the comparative brown rice in both of the two types of taste analysis instruments capable of measuring the fatty acid content. The result was lower.
[Example 2]

The rice seeds according to Example 2 were grown in a test field in the northwestern part of Akita Prefecture (Ogata Village) using seeds obtained from the rice seed strain (F8 generation) according to Example 1 as "F9 generation". Obtained. The cultivation method for the rice seeds according to Example 2 from the seeds of the F9 generation was the same as the normal cultivation method for paddy rice (cultivation method for Akitakomachi). Various observations were carried out on the rice seed strain (F9 generation) and the seeds (F10 generation) obtained from the strain according to Example 2 as follows.

In the seeds (F10 generation) according to Example 2, brown rice had a large grain trait as compared with the comparative cultivar Akitakomachi, as in the case of the rice seeds according to Example 1. The grains of large brown rice according to Example 2 were "long" with a length of about 6.3 mm and "wide" with a width of about 3.0 mm. The 1000-grain weight of the large-grain brown rice according to Example 2 was about 33.0 g, which was "large".

Similar to the rice seeds according to Example 1, the rice seeds according to Example 2 had an early heading period and a late maturity period of 2 days when planted at the same time as the comparative varieties of Akitakomachi. .. The heading period of the rice seeds according to Example 2 was "quite early" and the maturity period was "slightly early", as in the case of the rice seeds according to Example 1.

Further, the rice varieties according to Example 2 had a thick culm as compared with the comparative cultivar Akitakomachi, and the culms could be evaluated as "thick", as in the case of the rice varieties according to Example 1. Similar to the rice seeds according to Example 1, the rice seeds according to Example 2 had bent ears, but the lodging resistance was "strong".

The paddy yield of rice seeds (F10 generation) according to Example 2 was 828.75 kg / 10a. When brown rice is made from normal paddy, the weight is about 80%, so that the 1000-grain weight of brown rice according to Example 2 can be calculated as 663 kg / 10a. According to the fixed point yield of 2013 (Agricultural Technology Information No.7, supervision: Agricultural Promotion Division, Agriculture and Forestry Department, Senboku Regional Development Bureau, issued in December 2013), the yield of the comparative variety Akitakomachi is 591 kg / weight of brown rice. It can be said that it is usually a little less than 600 kg / 10a, such as 10a. It was determined that the rice seeds according to Example 2 had a clearly higher yield than the comparative varieties Akitakomachi. The rice seed strain according to Example 2 was of the F9 generation, but it was common to the strains of the F6 generation and later, and the yield was higher than that of the comparative variety Akitakomachi.
[Example 3]

The rice seeds according to Example 3 are obtained by growing seeds obtained from the rice seed strain (F8 generation) according to Example 1 as "F9 generation" in a test field in the southern part of Akita Prefecture (Yuzawa City). Was done. The growing method for the rice seeds according to Example 3 from the seeds of the F9 generation was the same as the normal paddy rice cultivation method (Akitakomachi cultivation method). For the seeds (F10 generation) obtained from the rice seed strain according to Example 3, the paddy yield was 783.9 kg / 10a. When calculated in the same manner as the rice species according to Example 2, the 1000-grain weight of brown rice according to Example 3 can be calculated to be 627.12 kg / 10a. The rice seeds according to Example 3 had a clearly higher yield than the comparative varieties Akitakomachi, similar to the rice seeds according to Example 2.
[Example 4]

The rice seeds according to Example 4 were obtained by growing seeds obtained from the rice seed strain (F8 generation) according to Example 1 as "F9 generation" in a test field in Ninohe City, Iwate Prefecture. The growing method for the rice seeds according to Example 4 from the seeds of the F9 generation was the same as the normal paddy rice cultivation method (Akitakomachi cultivation method). The yield of seeds (F10 generation) obtained from the rice seed strain according to Example 4 was 848.25 kg / 10a. When calculated in the same manner as the rice species according to Example 2, the 1000-grain weight of brown rice according to Example 4 can be calculated to be 678.6 kg / 10a. The rice seeds according to Example 4 had a clearly higher yield than the comparative varieties Akitakomachi, similar to the rice seeds according to Example 2.

(Other embodiments)
As mentioned above, the present invention has been described in accordance with the above embodiments and examples, but the statements and drawings that form part of this disclosure should not be understood as limiting the invention. This disclosure will reveal to those skilled in the art various alternative embodiments, examples and operational techniques.

As described above, it goes without saying that the present invention includes various embodiments not described here. Therefore, the technical scope of the present invention is defined only by the matters specifying the invention relating to the scope of claims, which can be interpreted as appropriate from the above description.

FERM BP-22401

1 ... (rice seed) strain 2 ... (rice seed) ear 11 ... stop leaf (sixth leaf)
13 ... 5th leaf 15 ... 4th leaf 17 ... 3rd leaf 19 ... 2nd leaf 21 ... 1st leaf 23 ... (rice seed) young ear 31a ... (second leaf) petiole 33a ... (second leaf) ) Leaf sheath 33b ... (third leaf) leaf sheath 35 ... (rice species) node 37 ... (rice species) 稈

Claims (7)

A rice species identified by accession number FERM BP-22401 or a successor strain. The glutamic acid content in refined brown rice is 1.1 times or more that of refined brown rice obtained from other varieties, Akitakomachi.
The aspartic acid content in the refined brown rice is 1.3 times or more that of the refined brown rice obtained from the above-mentioned Akitakomachi.
The rice seed according to claim 1, wherein the milled brown rice has a 1000-grain weight of 31 to 34 g. The rice species according to claim 1 or 2, wherein the rice species is obtained through isolation breeding and self-breeding of a mutant of the original species using Akitakomachi as the original species. The first polished rice obtained by milling the first polished brown rice obtained from the rice seed specified by the accession number FERM BP-22401 or the rice seed which is a progeny line, and
A second polished rice obtained by milling a second milled brown rice obtained from a rice seed of a variety different from that of the first polished rice, which was mixed at a desired ratio with respect to the number of the first polished rice. When,
A mixed rice comprising the first polished rice and a packaging container for storing the second polished rice. The glutamic acid content in the first refined brown rice is 1.1 times or more that of the refined brown rice obtained from other varieties Akitakomachi.
The aspartic acid content in the first refined brown rice is 1.3 times or more that of the refined brown rice obtained from the Akitakomachi.
The mixed rice according to claim 4, wherein the first milled brown rice has a 1000-grain weight of 31 to 34 g. The mixed rice according to claim 4 or 5, wherein the second refined brown rice is obtained from Akitakomachi. Any one of claims 4 to 6, wherein the rice species specified by the accession number FERM BP-22401 is obtained through Akitakomachi as the original species, and the mutants of the original species are isolated and bred and self-breeding. The mixed rice described in.

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