CN107148906A - A kind of selection without branch rape new material - Google Patents

Abstract

The invention discloses a method for breeding new materials of non-branched rapeseed. The method comprises the following steps: using Zhongshuang 11 as a woody plant, 506C as a male parent for hybridization; continuous selfing for four generations from the second year to the fifth year, According to the quality analysis in the laboratory, a single plant with an erucic acid content of less than 1% and a glucosinolate content of less than 30 μmol/g was selected for sowing in autumn, and by analogy to the fifth year; a total of 399 plants were found in two pedigrees in the fifth year. 342 plants of normal rapeseed, 43 plants of less-branched rapeseed, 14 plants of no-branched rapeseed, selfing of less-branched and no-branched rapeseed in the same year; selfing of no-branched rapeseed in the fifth to seventh year There is a new material of unbranched rapeseed which is self-homozygous, the field number is 697, the plant height is 84cm, there are 42 siliques per plant, and the thousand-grain weight is 4.9g. The erucic acid content is 0.24%, the glucosinolate content is 18.21 μmol/g, and the oil content is 50.23%. The new material of unbranched rape is hereditary.

Description

A kind of breeding method of new material of non-branched rapeseed

technical field

The invention belongs to the field of agricultural technology, and in particular relates to a method for breeding new materials of unbranched rapeseed.

Background technique

The rape plants currently cultivated in my country are relatively tall, with a general plant height of 170-200cm, an effective branch number of 8-12, a flowering period of 25-30 days, a single plant of 250-400 siliques, and a thousand-grain weight of 3.2-3.8g. Easy to lodging, inconsistent maturity, not conducive to mechanized sowing and harvesting.

Contents of the invention

The purpose of the present invention is to provide a method for breeding new materials of non-branched rapeseed. The non-branched rapeseed selected by the method has no branches when mature, only the main inflorescence blooms and bears siliques, and the plants are short and generally high. 80-100cm, the flowering period is 7-10 days, the number of siliques per plant is about 40, the thousand-grain weight is 3.8-5.9g, the planting density can reach more than 100,000 plants/mu, it is not easy to lodging, and it is conducive to mechanized sowing and harvesting.

Its specific technical plan is:

A method for breeding a new variety of non-branched rapeseed, comprising the following steps: in the first year, Zhongshuang 11 is used as the female parent, and the restorer line 506C is used as the male parent for hybridization; In the fifth year, a total of 399 strains of two pedigrees were found, including 342 strains of normal rapeseed, 43 strains of less-branched rapeseed, and 14 strains of non-branched rapeseed. The non-branched new material is homozygous for selfing. By the seventh year of autumn sowing, there have been more than 200 copies of the new material of the non-branched rapeseed, and 10 sterile plants of the non-branched rapeseed have also been found.

Step 1. Use Zhongshuang 11 as the woody parent and 506C as the male parent;

Step 2. Continuous selfing for four generations from the second year to the fifth year. After laboratory quality analysis, select a single plant with an erucic acid content of less than 1% and a glucosinolate content of less than 30 μmol/g for sowing in autumn, and analogize it to the fifth year;

Step 3, find that there are 399 strains in total of two pedigrees in the fifth year, 342 strains of normal rapeseed, 43 strains of less-branched rapeseed, 14 strains of non-branched rapeseed, selfing is carried out to few-branched and non-branched rapeseed in that year;

Step 4. In the seventh year, there is a self-cross homozygous new material without branches. By the autumn sowing of this year, there have been more than 200 new materials of rape without branches, and 10 sterile plants of rapeseed with no branches.

The unbranched rapeseed was completed in the Xixiang Rapeseed Base of Northwest Agriculture and Forestry University. At that time, the purpose was to use Zhongshuang 11 to improve the 506C restorer line we selected to improve anti-sclerotinia and lodging resistance. The two parents were self-bred to homozygous for many years, and no branched rape was found. After parental crossing, new materials without branches were produced in the selfed offspring, but when the two parents were crossed with other materials, no branched rapeseed was found. Using Zhongshuang 11 and the 506C restorer line to cross again, unbranched rape can also appear repeatedly in the self-bred offspring, which shows that one of the parents contains the recessive gene of unbranched rape. For the specific reason, we have organized relevant personnel Conduct research in related fields.

The plant height of unbranched rapeseed is 80-100cm, the flowering period is 7-10 days, the number of siliques per plant is about 40, the number of siliques per silique is 20-25, the thousand-grain weight is 3.8-5.9g, the content of erucic acid is 0.19-0.58%, sulfur The glycoside content is 19.39-25.67 μmol/g, and the oil content is 45-54%.

Compared with prior art, the beneficial effect of the present invention:

The non-branched rapeseed bred by the method of the present invention has no branches when mature, only the main inflorescence bears siliques, the plant is short, the general plant height is 80-100cm, the flowering period is 7-10 days, and the number of siliques per plant is about 40 , 1,000-grain weight 3.8-5.9g, planting density can reach more than 100,000 plants/mu, not easy to lodging, planting density can reach 100,000-150,000 plants/mu, flowering period is more than 20 days shorter than ordinary rapeseed, harvesting period is shortened by 7-10 days, It can be sown and mechanically sown, and the yield per mu can be estimated to be more than 250kg, which is conducive to mechanized sowing and harvesting.

Description of drawings

Fig. 1 is the flowchart of the breeding method of the new material of unbranched rapeseed of the present invention.

detailed description

The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

On May 15, 2014, when recording the characters of siliques and investigating diseases in the rape test field of Northwest Agriculture and Forestry University in Zaoyuan Village, Xixiang County, Shaanxi Province, a Brassica oleracea species was found in the inbred line material numbered 969-974. A total of 14 branches of rapeseed were found. The source of the material is the self-crossing of Zhongshuang 11/506C hybrid offspring, in which the female parent Zhongshuang 11 is a new Brassica double-low conventional rape variety bred by the Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, which is a homozygous line of our selfing. Its erucic acid content is 0.0%, the cake glucosinolate content is 23.49μmol/g, and the oil content is 47.52%. The male parent 506C is a new Brassica napus double-low conventional line selected by us, and it is also a cold-resistant and drought-tolerant restorer line. Its erucic acid content is 0.29%, the cake glucosinolate content is 21.37μmol/g, and the oil content is 48.12%. But in many years of selfing, these two parents have never found new materials of unbranched rapeseed.

Referring to Figure 1, in 2010, Zhongshuang 11 was used as the female parent, and the restorer line 506C was used as the male parent. Four consecutive generations of self-crossing were carried out from 2011 to 2014. In 2014, two pedigrees were found with a total of 399 plants, 342 normal rapeseed plants, and a few 43 plants of branched rapeseed and 14 plants of non-branched rapeseed. Selfing was carried out on few-branched and non-branched rapeseed in that year. In 2016, a new material without branches was self-crossed homozygously. By 2016, there were no-branched rapeseed in the autumn sowing More than 200 copies of new materials and 10 sterile rapeseed plants without branches.

(1) Using Zhongshuang 11 as the female parent and the restorer line 506C as the male parent, the new material of rapeseed without branches can appear repeatedly in the selfed offspring. It shows that among the parents of non-branched rapeseed, one parent contains the recessive gene of non-branched rape; The generation of non-branched rapeseed is homozygous, indicating that it is hereditary and can be used for breeding new varieties of non-branched rapeseed. (3) The grown unbranched rape has a plant height of 84cm, about 40 siliques per plant, and a thousand-grain weight of 4.9g. The erucic acid content is 0.24%, the glucosinolate content is 18.21 μmol/g, and the oil content reaches 50.23%. It is a new germplasm of Brassica napus with innovative value at present; (4) Rapeseed without branches has short plants, large grain size, high oil content, planting density of more than 100,000 plants/mu, and is not easy to lodging. Ordinary rape is shortened by more than 20 days, and the harvest period is shortened by 7-10 days. It can be broadcast and mechanically sown, and can also be delayed. The yield per mu can reach more than 250kg. The maturity period is consistent, which is conducive to mechanized sowing and harvesting.

The above is only a preferred specific embodiment of the present invention, and the scope of protection of the present invention is not limited thereto. Any person familiar with the technical field within the technical scope disclosed in the present invention can obviously obtain the simplicity of the technical solution. Changes or equivalent replacements all fall within the protection scope of the present invention.

Claims (1)

1. a method for selecting and breeding new materials of non-branched rape, is characterized in that, comprises the following steps: Step 1. Use Zhongshuang 11 as the woody parent and 506C as the male parent; Step 2. Continuous selfing for four generations from the second year to the fifth year. After laboratory quality analysis, select a single plant with an erucic acid content of less than 1% and a glucosinolate content of less than 30 μmol/g for sowing in autumn, and analogize it to the fifth year; Step 3, find that there are 399 strains in total of two pedigrees in the fifth year, 342 strains of normal rapeseed, 43 strains of less-branched rapeseed, 14 strains of non-branched rapeseed, selfing is carried out to few-branched and non-branched rapeseed in that year; Step 4. In the seventh year, there is a self-cross homozygous new material without branches. By the autumn sowing of this year, there have been more than 200 new materials of rape without branches, and 10 sterile plants of rapeseed with no branches.