CN108450236A - A kind of culture medium and preparation method thereof for flat mushroom strain culture - Google Patents
A kind of culture medium and preparation method thereof for flat mushroom strain culture Download PDFInfo
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- CN108450236A CN108450236A CN201810090251.1A CN201810090251A CN108450236A CN 108450236 A CN108450236 A CN 108450236A CN 201810090251 A CN201810090251 A CN 201810090251A CN 108450236 A CN108450236 A CN 108450236A
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- culture medium
- sawdust
- culture
- asiaticoside
- peptone
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- Chemical & Material Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention belongs to fungus growing technique fields, and in particular to a kind of culture medium for flat mushroom strain culture is mainly made of the raw material of following weight percent:Sawdust 3~10%, sucrose 1~5%, peptone 2~8%, menthol 0.1~1%, asiaticoside 0.1~0.8%, ginkgo biloba p.e 0.05~0.5%, semen armeniacae amarae 0.02~0.5%, ammonium sulfate 0.01~0.4%, dipotassium hydrogen phosphate 0.5~4%, potassium dihydrogen phosphate 0.5~4%, agar 1~5%, surplus are water.The preparation method of the culture medium includes the following steps:Each raw material is weighed according to the ratio, and soluble in water, sterilize 15~40 minutes under conditions of 0.1MPa, 121 DEG C, obtain culture medium.The culture medium of gained of the invention improves mycelial growth rate and mycelium growth vigor.
Description
Technical field
The invention belongs to fungus growing technique fields, and in particular to a kind of culture medium for flat mushroom strain culture and its
Preparation method.
Background technology
Oyster mushroom (Pleurotus ostreatus) also referred to as picks up the ears, oyster cap fungus, oyster mushroom, black tree peony mushroom, TaiWan, China is also known as
Elegant precious mushroom is one type of Agaricales Pleurotaceae under Basidiomycota, is that kind of a fairly common grey eats mushroom.
With the development of mushroom industry, oyster mushroom is production-scale to be expanded year by year.At the same time, the demand of flat mushroom strain
Also synchronous to increase, the culture medium of Spawn incubation affects the quality of strain, and the quality of strain directly affects the matter for cultivating oyster mushroom
Amount.
Application publication number is CN 107382501A, and data of publication of application is the Chinese invention patent Shen on November 24th, 2017
Please, it discloses a kind of wild mushroom culture medium and preparation method thereof, is grouped as by the group of following mass component:Sawdust 50~70
Part, 5~15 parts of disodium hydrogen phosphate, 20~30 parts of silkworm excrement, 5~10 parts of ionized calcium solvent, 5~15 parts of rape shell stem, tea grounds 10~
30 parts, 5~10 parts of ferric sulfate, 10~30 parts of rice bran, 5~10 parts of gibberellin.The culture substrate can shorten cultivation period number of days.
Application publication number is CN 106478182A, and data of publication of application is the Chinese invention patent application on March 8th, 2017,
It discloses a kind of culture mediums of oyster mushroom, are made of following part raw material:20-30 parts of soybean stalks, 20-40 parts of drying mud powder,
15-25 parts of wheat bran, 5-15 parts of organic fertilizers, 1-2 parts of pulverized limestones, 3-5 parts of composite fertilizers, 2-3 portions of vinegar, the culture base manure of the invention oyster mushroom
Power is good, and nutritional ingredient is comprehensive, can make oyster mushroom finished product time advance.
Above two culture medium can shorten cultivation period, but obviously not imitated in terms of mycelial growth rate and growing way
Fruit.
Invention content
One aspect of the present invention technical problems to be solved are to provide a kind of culture medium for flat mushroom strain culture, to carry
High mycelial growth rate and mycelium growth vigor.
In order to solve the above-mentioned technical problem, the technical scheme is that:
A kind of culture medium for flat mushroom strain culture is mainly made of the raw material of following weight percent:Sawdust 3~
10%, sucrose 1~5%, peptone 2~8%, menthol 0.1~1%, asiaticoside 0.1~0.8%, ginkgo biloba p.e
0.05~0.5%, semen armeniacae amarae 0.02~0.5%, ammonium sulfate 0.01~0.4%, dipotassium hydrogen phosphate 0.5~4%, biphosphate
Potassium 0.5~4%, agar 1~5%, surplus are water.
In the culture medium provided by the present invention for flat mushroom strain culture, it is preferable that mainly by following weight percent
Raw material be made:Sawdust 5~8%, sucrose 1~3%, peptone 2~5%, menthol 0.1~0.5%, asiaticoside 0.2
~0.6%, ginkgo biloba p.e 0.1~0.5%, semen armeniacae amarae 0.02~0.1%, ammonium sulfate 0.01~0.2%, phosphoric acid hydrogen two
Potassium 1~3%, potassium dihydrogen phosphate 1~3%, agar 2~3%, surplus are water.
In the culture medium provided by the present invention for flat mushroom strain culture, it is preferable that mainly by following weight percent
Raw material be made:Sawdust 5%, sucrose 2%, peptone 2.5%, menthol 0.2%, asiaticoside 0.3%, ginkgo leaf extraction
Object 0.1%, semen armeniacae amarae 0.05%, ammonium sulfate 0.06%, dipotassium hydrogen phosphate 2%, potassium dihydrogen phosphate 2%, agar 2%, surplus are
Water.
In the culture medium provided by the present invention for flat mushroom strain culture, it is preferable that the sawdust is willow sawdust.
The technical problem to be solved is that provide a kind of culture medium for flat mushroom strain culture for another aspect of the present invention
Preparation method, include the following steps:
S1, sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, hardship are weighed by weight percentage
Almond, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, agar and water;
S2, by sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, semen armeniacae amarae, ammonium sulfate, phosphorus
Sour hydrogen dipotassium, potassium dihydrogen phosphate and agar are soluble in water, obtain solution A;
S3, solution A is sterilized 15~40 minutes under conditions of 0.1MPa, 121 DEG C, obtains culture medium.
In the preparation method provided by the present invention for the culture medium of flat mushroom strain culture, it is preferable that the step S3
Middle sterilization time is 30 minutes.
Using above-mentioned technical proposal, due to sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e,
The rational proportion of semen armeniacae amarae, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and agar so that with the strain of this medium culture
Mycelium growth vigor is strong, shows as that mycelia is white and dense, and mycelial growth rate is fast.Asiaticoside and semen armeniacae amarae are per day to improving mycelia
The speed of growth and mycelium growth vigor act synergistically with synergy.
Specific implementation mode
The specific implementation mode of the present invention is described further below.It should be noted that for these implementations
The explanation of mode is used to help understand the present invention, but does not constitute limitation of the invention.In addition, invention described below
Involved technical characteristic can be combined with each other as long as they do not conflict with each other in each embodiment.
Embodiment 1
A kind of culture medium for flat mushroom strain culture is present embodiments provided, mainly by the raw material of following weight percent
It is made:Sawdust 10%, sucrose 5%, peptone 2%, menthol 0.1%, asiaticoside 0.8%, ginkgo biloba p.e
0.05%, semen armeniacae amarae 0.02%, ammonium sulfate 0.4%, dipotassium hydrogen phosphate 4%, potassium dihydrogen phosphate 4%, agar 5%, surplus are water.
The preparation method of culture medium for flat mushroom strain culture, includes the following steps:
S1, sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, hardship are weighed by weight percentage
Almond, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, agar and water;
S2, by sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, semen armeniacae amarae, ammonium sulfate, phosphorus
Sour hydrogen dipotassium, potassium dihydrogen phosphate and agar are soluble in water, obtain solution A;
S3, solution A is sterilized 15 minutes under conditions of 0.1MPa, 121 DEG C, obtains culture medium.
Embodiment 2
A kind of culture medium for flat mushroom strain culture is present embodiments provided, mainly by the raw material of following weight percent
It is made:Sawdust 3%, sucrose 1%, peptone 8%, menthol 1%, asiaticoside 0.1%, ginkgo biloba p.e 0.5%, hardship
Almond 0.5%, ammonium sulfate 0.01%, dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.5%, agar 1%, surplus are water.
The preparation method of culture medium for flat mushroom strain culture, includes the following steps:
S1, sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, hardship are weighed by weight percentage
Almond, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, agar and water;
S2, by sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, semen armeniacae amarae, ammonium sulfate, phosphorus
Sour hydrogen dipotassium, potassium dihydrogen phosphate and agar are soluble in water, obtain solution A;
S3, solution A is sterilized 40 minutes under conditions of 0.1MPa, 121 DEG C, obtains culture medium.
Embodiment 3
A kind of culture medium for flat mushroom strain culture is present embodiments provided, mainly by the raw material of following weight percent
It is made:Sawdust 5%, sucrose 2%, peptone 2.5%, menthol 0.2%, asiaticoside 0.3%, ginkgo biloba p.e
0.1%, semen armeniacae amarae 0.05%, ammonium sulfate 0.06%, dipotassium hydrogen phosphate 2%, potassium dihydrogen phosphate 2%, agar 2%, surplus are water.
The preparation method of culture medium for flat mushroom strain culture, includes the following steps:
S1, sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, hardship are weighed by weight percentage
Almond, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, agar and water;
S2, by sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, semen armeniacae amarae, ammonium sulfate, phosphorus
Sour hydrogen dipotassium, potassium dihydrogen phosphate and agar are soluble in water, obtain solution A;
S3, solution A is sterilized 30 minutes under conditions of 0.1MPa, 121 DEG C, obtains culture medium.
Embodiment 4
A kind of culture medium for flat mushroom strain culture is present embodiments provided, mainly by the raw material of following weight percent
It is made:Sawdust 6%, sucrose 3%, peptone 3%, menthol 0.3%, asiaticoside 0.5%, ginkgo biloba p.e
0.08%, semen armeniacae amarae 0.04%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 1%, potassium dihydrogen phosphate 1%, agar 3%, surplus are water.
The preparation method of culture medium for flat mushroom strain culture, includes the following steps:
S1, sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, hardship are weighed by weight percentage
Almond, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, agar and water;
S2, by sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, semen armeniacae amarae, ammonium sulfate, phosphorus
Sour hydrogen dipotassium, potassium dihydrogen phosphate and agar are soluble in water, obtain solution A;
S3, solution A is sterilized 20 minutes under conditions of 0.1MPa, 121 DEG C, obtains culture medium.
Embodiment 5
A kind of culture medium for flat mushroom strain culture is present embodiments provided, mainly by the raw material of following weight percent
It is made:Sawdust 5%, sucrose 3%, peptone 2.5%, menthol 0.2%, asiaticoside 0.3%, ginkgo biloba p.e
0.1%, semen armeniacae amarae 0.05%, ammonium sulfate 0.06%, dipotassium hydrogen phosphate 2%, potassium dihydrogen phosphate 2%, agar 2%, motherwort stream
Medicinal extract 0.03%, surplus are water.
The preparation method of culture medium for flat mushroom strain culture, includes the following steps:
S1, sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, hardship are weighed by weight percentage
Almond, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, agar, ext leonuri sibirici liq and water;
S2, by sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, semen armeniacae amarae, ammonium sulfate, phosphorus
Sour hydrogen dipotassium, potassium dihydrogen phosphate, agar and ext leonuri sibirici liq are soluble in water, obtain solution A;
S3, solution A is sterilized 30 minutes under conditions of 0.1MPa, 121 DEG C, obtains culture medium.
Embodiment 6
A kind of culture medium for flat mushroom strain culture is present embodiments provided, mainly by the raw material of following weight percent
It is made:Sawdust 4%, sucrose 2%, peptone 3%, menthol 0.2%, ginkgo biloba p.e 0.1%, semen armeniacae amarae 0.05%, sulphur
Sour ammonium 0.06%, dipotassium hydrogen phosphate 2%, potassium dihydrogen phosphate 2%, agar 2%, surplus are water.
The preparation method of culture medium for flat mushroom strain culture, includes the following steps:
S1, sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, hardship are weighed by weight percentage
Almond, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, agar and water;
S2, by sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, semen armeniacae amarae, ammonium sulfate, phosphorus
Sour hydrogen dipotassium, potassium dihydrogen phosphate and agar are soluble in water, obtain solution A;
S3, solution A is sterilized 30 minutes under conditions of 0.1MPa, 121 DEG C, obtains culture medium.
Embodiment 7
A kind of culture medium for flat mushroom strain culture is present embodiments provided, mainly by the raw material of following weight percent
It is made:Sawdust 5%, sucrose 2%, peptone 4%, menthol 0.2%, asiaticoside 0.5%, ginkgo biloba p.e 0.1%,
Ammonium sulfate 0.06%, dipotassium hydrogen phosphate 2%, potassium dihydrogen phosphate 2%, agar 2%, surplus are water.
The preparation method of culture medium for flat mushroom strain culture, includes the following steps:
S1, sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, hardship are weighed by weight percentage
Almond, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, agar and water;
S2, by sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, semen armeniacae amarae, ammonium sulfate, phosphorus
Sour hydrogen dipotassium, potassium dihydrogen phosphate and agar are soluble in water, obtain solution A;
S3, solution A is sterilized 30 minutes under conditions of 0.1MPa, 121 DEG C, obtains culture medium.
Test example
In order to verify the advantageous effects of the present invention, flat mushroom strain is carried out using the culture medium for implementing 1 to 7 gained respectively
Culture, investigates the per day speed of growth of its mycelia and mycelium growth vigor situation, the results are shown in Table 1.
The per day speed of growth of mycelia and mycelium growth vigor of medium culture flat mushroom strain obtained by each embodiment of table 1.
As can be seen from the above table, with the per day growth speed of the mycelia of the flat mushroom strain of 1 to 5 gained medium culture of embodiment
Degree is significantly higher than the per day speed of growth of hypha of Pleurotus ostreatus with embodiment 6 and 7 gained medium cultures;With 1 to 5 gained of embodiment
The oyster mushroom of medium culture, mycelium growth vigor are better than the oyster mushroom with embodiment 6 and 7 gained medium cultures.In the training of embodiment 6
It supports in base constitutive material and lacks asiaticoside, lack semen armeniacae amarae in the culture medium constitutive material of embodiment 7, it is seen then that accumulated snow
Careless total glycosides and semen armeniacae amarae act synergistically to improving the per day speed of growth of hypha of Pleurotus ostreatus and mycelium growth vigor with synergy.
Have mycelia growth normal with the flat mushroom strain of 1 to 5 gained medium culture of embodiment and growing way is strong, shows
It is white and dense for mycelia, the fast advantage of mycelial growth rate.Lack asiaticoside in the culture medium constitutive material of embodiment 6
Embodiments of the present invention are explained in detail above, but the present invention is not limited to described embodiments.It is right
For those skilled in the art, in the case where not departing from the principle of the invention and spirit, these embodiments are carried out more
Kind change, modification, replacement and modification, still fall in protection scope of the present invention.
Claims (6)
1. a kind of culture medium for flat mushroom strain culture, which is characterized in that be mainly made of the raw material of following weight percent:
Sawdust 3~10%, sucrose 1~5%, peptone 2~8%, menthol 0.1~1%, asiaticoside 0.1~0.8%, ginkgo
Leaf extract 0.05~0.5%, semen armeniacae amarae 0.02~0.5%, ammonium sulfate 0.01~0.4%, dipotassium hydrogen phosphate 0.5~4%, phosphorus
Acid dihydride potassium 0.5~4%, agar 1~5%, surplus are water.
2. the culture medium according to claim 1 for flat mushroom strain culture, which is characterized in that mainly by following weight hundred
The raw material of ratio is divided to be made:Sawdust 5~8%, sucrose 1~3%, peptone 2~5%, menthol 0.1~0.5%, asiaticoside
0.2~0.6%, ginkgo biloba p.e 0.1~0.5%, semen armeniacae amarae 0.02~0.1%, ammonium sulfate 0.01~0.2%, phosphoric acid hydrogen
Dipotassium 1~3%, potassium dihydrogen phosphate 1~3%, agar 2~3%, surplus are water.
3. the culture medium according to claim 2 for flat mushroom strain culture, which is characterized in that mainly by following weight hundred
The raw material of ratio is divided to be made:Sawdust 5%, sucrose 2%, peptone 2.5%, menthol 0.2%, asiaticoside 0.3%, ginkgo leaf
Extract 0.1%, semen armeniacae amarae 0.05%, ammonium sulfate 0.06%, dipotassium hydrogen phosphate 2%, potassium dihydrogen phosphate 2%, agar 2%, it is remaining
Amount is water.
4. being used for the culture medium of flat mushroom strain culture according to claims 1 to 3 any one of them, which is characterized in that the saw
End is willow sawdust.
5. a kind of preparation method of culture medium such as claims 1 to 3 any one of them for flat mushroom strain culture, feature
It is, includes the following steps:
S1, sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, bitter apricot are weighed by weight percentage
Benevolence, ammonium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, agar and water;
S2, by sawdust, sucrose, peptone, menthol, asiaticoside, ginkgo biloba p.e, semen armeniacae amarae, ammonium sulfate, phosphoric acid hydrogen
Dipotassium, potassium dihydrogen phosphate and agar are soluble in water, obtain solution A;
S3, solution A is sterilized 15~40 minutes under conditions of 0.1MPa, 121 DEG C, obtains culture medium.
6. the preparation method of the culture medium according to claim 5 for flat mushroom strain culture, which is characterized in that the step
Sterilization time is 30 minutes in rapid S3.
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Cited By (2)
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CN109362487A (en) * | 2018-12-12 | 2019-02-22 | 吕梁学院 | A kind of oyster cap fungus culture medium and preparation method thereof |
CN111165273A (en) * | 2020-02-26 | 2020-05-19 | 河南城建学院 | Culture method and culture medium for high-yield selenium-rich cordyceps militaris |
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