CN108434304A - The preparation method of cancer-resisting Chinese medicine - Google Patents
The preparation method of cancer-resisting Chinese medicine Download PDFInfo
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- CN108434304A CN108434304A CN201810503999.XA CN201810503999A CN108434304A CN 108434304 A CN108434304 A CN 108434304A CN 201810503999 A CN201810503999 A CN 201810503999A CN 108434304 A CN108434304 A CN 108434304A
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation method of cancer-resisting Chinese medicine, the preparation method of the cancer-resisting Chinese medicine includes the following steps：(1) mixed powder A is prepared；(2) mixed powder B is prepared；(3) it digests；(4) water carries；(5) mixing, preparation.The preparation method of cancer-resisting Chinese medicine of the present invention, obtained cancer-resisting Chinese medicine can effectively enhance immunity of organisms and muscle power, inhibit growth of cancer cells, prevent cancer cell from spreading and being mutated, and active constituent content is high, is easy to absorb, mitigates the pain of cancer patient.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicines, and in particular to a kind of preparation method of cancer-resisting Chinese medicine.
Background technology
Cancer is to endanger one of the disease of human health most serious, is referred to as incurable disease.Cancer is a kind of life style disease,
The reason of morbidity is mostly that inside and outside conjunction is evil, on the basis of itself constitution of people, feelings will, diet, life etc. are lacked of proper care, by the external world
Various pessimal stimulations such as society, environment, psychology, collective effect cause gene mutation, lead to the result of abnormal cell division.
Theoretically from Chinese medical discrimination, then it is solidifying, heat toxin of various asthenic symptoms, the stagnation of the circulation of vital energy, blood stasis, phlegm etc. caused by the deficiency of vial QI of people
Heresy cohesion it is cementing form, be energy and blood of human body to seize up caused by stasis, be performance of the systemic disease in part, it is not
Different card types can be shown with stadium different phase.Chinese medicine treating cancer with clearing heat and detoxicating, promoting blood circulation and removing blood stasis, dredging the channel, is dispelled
The rules such as wet broken heavily fortified point, element culturing fixed folder.
Currently, the therapy of cancer, mainly based on the operation of doctor trained in Western medicine, chemotherapy and radiation, these methods are in current cancer
Treatment in played positive effect.But the excessive attack such as chemotherapy and radiation of Super-radical excision, large dosage in operation
Property treatment do not play the role of not only extend patient survival, reduce the quality of life of patient instead, make the symptom of patient
It aggravates, as digestive tract reaction (loss of appetite, Nausea and vomiting), bone marrow suppression (Neuroleptic Leukocytopenia, anaemia), organ dysfunction are impaired
Deng also resulting in most of patient cannot adhere to and complete to treat, final treatment failure.There are many drug of doctor trained in Western medicine cancer, but big
Part can only alleviate symptom, and side effect is larger.
In recent years, in China, it has been found that the effect of Chinese medicine cancer-resisting is better than the therapy of doctor trained in Western medicine, and can subtract
Light patient suffering improves the quality of life of patient.
Invention content
Aiming at the above shortcomings existing in the prior art, technical problem to be solved by the invention is to provide a kind of anti-cancer is anti-
The preparation method of cancer Chinese medicine, obtained cancer-resisting Chinese medicine, can be improved immunity of organisms, inhibit the upper growth and expansion of cancer cell
It dissipates, the formation of pre- anti-cancer, and treats kinds cancer, alleviate and eliminate radiotherapy or the side effect of chemotherapy brought, keep fit and healthy,
Improve the quality of life of patient.
Purpose of the present invention is to what is be achieved through the following technical solutions：
A kind of preparation method of cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 5-10g, rainbow conk 5-10g are crushed respectively, 40 mesh sieve is crossed, 200- is added after mixing
400g water carries 2-4 hours in 85-95 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate, close when filtrate decompression is concentrated into 60 DEG C
Degree is the clear cream of 1.10-1.15g/mL, and clear cream is dried at 50-70 DEG C to constant weight, is crushed, 300 mesh sieve is crossed, obtains mixed powder A；
(2) mixed powder B is prepared：By hairyvein agrimony 20-30g, Radix Astragali 15-25g, Sculellaria barbata 15-25g, oldenlandia diffusa 10-
20g, Poria cocos 10-20g, salvia chinensis 8-12g, Chinese violet 8-12g, gynostemma pentaphylla 8-12g, dandelion 5-10g, Radix Glycyrrhizae 5-10g, point
It does not crush, crosses 40 mesh sieve, mixing obtains mixed powder B；
(3) it digests：The water of 7-14 times of mixed powder B mass is added in mixed powder B, mixed powder B mass 0.1- is added after mixing
0.3% cellulase is stirred 60-90 minutes with 100-200 revs/min at 40-50 DEG C, obtains mixed liquor C；
(4) water carries：Mixed liquor C is carried 60-90 minutes in 90-98 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter
The water of 6-10 times of mixed powder B mass is added in filter residue, is carried 40-60 minutes in 90-98 DEG C of water for slag, using 300 mesh filter-cloth filterings,
Liquor B, merging filtrate A and liquor B are obtained, liquor C is obtained, density when liquor C is concentrated under reduced pressure into 60 DEG C is 1.10-1.15g/mL
Clear cream, clear cream is dried at 50-70 DEG C to constant weight, is crushed, 300 mesh sieve is crossed, obtains mixed powder C；
(5) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.01-0.1g are mixed, routinely technique preparation, obtains
To cancer-resisting Chinese medicine.
Preferably, the preparation method of the cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 5-10g, rainbow conk 5-10g are crushed respectively, 40 mesh sieve is crossed, 200- is added after mixing
400g water carries 2-4 hours in 85-95 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate, close when filtrate decompression is concentrated into 60 DEG C
Degree is the clear cream of 1.10-1.15g/mL, and clear cream is dried at 50-70 DEG C to constant weight, is crushed, 300 mesh sieve is crossed, obtains mixed powder A；
(2) mixed powder B is prepared：By hairyvein agrimony 20-30g, Radix Astragali 15-25g, Sculellaria barbata 15-25g, oldenlandia diffusa 10-
20g, Poria cocos 10-20g, salvia chinensis 8-12g, Chinese violet 8-12g, gynostemma pentaphylla 8-12g, dandelion 5-10g, Radix Glycyrrhizae 5-10g, point
It does not crush, crosses 40 mesh sieve, mixing obtains mixed powder B；
(3) freeze-thaw is handled：The water of 2-4 times of mixed powder B mass is added in mixed powder B, is stirred with 60-100 revs/min
10-20 minutes, 30-60 minutes are stood at 20-30 DEG C, obtains mixed liquor A, it is small that mixed liquor A is freezed at -60~-20 DEG C to 1-3
When, it is ultrasonically treated 20-40 minutes after taking-up, then 30-50 minutes are stood at 25-35 DEG C, the mixed liquor to be thawed, by defrosting
Mixed liquor carries out freeze-thaw processing again under these conditions, obtains mixed liquid B；
(4) it digests：The water of 5-10 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass is added after mixing
The cellulase of 0.1-0.3% is stirred 60-90 minutes with 100-200 revs/min at 40-50 DEG C, obtains mixed liquor C；
(5) water carries：Mixed liquor C is carried 60-90 minutes in 90-98 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter
The water of 6-10 times of mixed powder B mass is added in filter residue, is carried 40-60 minutes in 90-98 DEG C of water for slag, using 300 mesh filter-cloth filterings,
Liquor B, merging filtrate A and liquor B are obtained, liquor C is obtained, density when liquor C is concentrated under reduced pressure into 60 DEG C is 1.10-1.15g/mL
Clear cream, clear cream is dried at 50-70 DEG C to constant weight, is crushed, 300 mesh sieve is crossed, obtains mixed powder C；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.01-0.1g are mixed, routinely technique preparation, obtains
To cancer-resisting Chinese medicine.
It is highly preferred that the preparation method of the cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 5-10g, rainbow conk 5-10g are crushed respectively, 40 mesh sieve is crossed, 200- is added after mixing
400g water carries 2-4 hours in 85-95 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate, close when filtrate decompression is concentrated into 60 DEG C
Degree is the clear cream of 1.10-1.15g/mL, and clear cream is dried at 50-70 DEG C to constant weight, is crushed, 300 mesh sieve is crossed, obtains mixed powder A；
(2) mixed powder B is prepared：By hairyvein agrimony 20-30g, Radix Astragali 15-25g, Sculellaria barbata 15-25g, oldenlandia diffusa 10-
20g, Poria cocos 10-20g, salvia chinensis 8-12g, Chinese violet 8-12g, gynostemma pentaphylla 8-12g, dandelion 5-10g, Radix Glycyrrhizae 5-10g, point
It does not crush, crosses 40 mesh sieve, mixing obtains mixed powder B；
(3) freeze-thaw is handled：The water of 2-4 times of mixed powder B mass is added in mixed powder B, is stirred with 60-100 revs/min
10-20 minutes, 30-60 minutes are stood at 20-30 DEG C, obtains mixed liquor A, it is small that mixed liquor A is freezed at -60~-20 DEG C to 1-3
When, it is ultrasonically treated 20-40 minutes after taking-up, then 30-50 minutes are stood at 25-35 DEG C, the mixed liquor to be thawed, by defrosting
Mixed liquor carries out freeze-thaw processing again under these conditions, obtains mixed liquid B；
(4) it digests：The water of 5-10 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass is added after mixing
The cellulase of 0.1-0.3% and the enzymolysis auxiliary agent of 0.1-0.5%, at 40-50 DEG C with 100-200 revs/min of 60-90 points of stirring
Clock obtains mixed liquor C；
(5) water carries：Mixed liquor C is carried 60-90 minutes in 90-98 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter
The water of 6-10 times of mixed powder B mass is added in filter residue, is carried 40-60 minutes in 90-98 DEG C of water for slag, using 300 mesh filter-cloth filterings,
Liquor B, merging filtrate A and liquor B are obtained, liquor C is obtained, density when liquor C is concentrated under reduced pressure into 60 DEG C is 1.10-1.15g/mL
Clear cream, clear cream is dried at 50-70 DEG C to constant weight, is crushed, 300 mesh sieve is crossed, obtains mixed powder C；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.01-0.1g are uniformly mixed, cancer-resisting is obtained
Chinese medicine.
Preferably, the operating condition of step (3) described supersound process is 50-70 DEG C of ultrasonic temperature, ultrasonic power 100-
300W, supersonic frequency 15-25KHz.
The enzymolysis auxiliary agent is one or both of glycine, Serine mixture.
Preferably, the enzymolysis auxiliary agent is the mixture of the glycine of 20-40wt% and the Serine of 60-80wt%.
The antistaling agent is one or both of Folium Isatidis extract and capillary extract mixture.
Preferably, the antistaling agent is the mixing of 20-30wt% Folium Isatidis extracts and 70-80wt% capillary extracts
Object.
The Folium Isatidis extract is prepared by following methods：Folium isatidis is crushed, 40 mesh sieve is crossed, obtains folium isatidis
The ethanol water that folium isatidis powder 40-60g and 500-1000mL volume fraction is 60-70% is mixed, is returned at 60-70 DEG C by powder
Stream extraction 2-4 hours, using 300 mesh filter-cloth filterings, obtains filtrate and filter residue, 300-500mL volume fractions is added in filter residue
For the ethanol water of 60-70%, in 60-70 DEG C of refluxing extraction 1-3 hours, using 300 mesh filter-cloth filterings, merging was filtered twice
Liquid is 60-70 DEG C in vacuum degree 0.05-0.1MPa, temperature and is concentrated under reduced pressure that the density for being concentrated into 65 DEG C is 1.05-
1.10g/mL, then dried to constant weight at 60-70 DEG C, it crushes, crosses 300 mesh sieve, obtain Folium Isatidis extract.
The capillary extract is prepared by following methods：Oriental wormwood is crushed, 40 mesh sieve is crossed, oriental wormwood powder is obtained, by mattress
Old powder 40-60g and the mixing of 500-1000mL water, are ultrasonically treated 10-20 minutes, supersonic frequency 15-25KHz, ultrasound at 60-70 DEG C
Power 100-200W, then extracted 60-90 minutes in 60-70 DEG C of water-bath, extraction process is stirred with 100-300 revs/min, using 300
Mesh filter-cloth filtering obtains oriental wormwood extracting solution, and density is the clear of 1.05-1.10g/mL when oriental wormwood extracting solution is concentrated under reduced pressure into 65 DEG C
Clear cream is dried at 65 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain capillary extract by cream.
Preferably, the capillary extract is prepared by following methods：Oriental wormwood is crushed, 40 mesh sieve is crossed, obtains oriental wormwood
Powder mixes oriental wormwood powder 40-60g and 500-1000mL water, and 0.8-1.2g sodium bicarbonates are added, and 10- is ultrasonically treated at 60-70 DEG C
20 minutes, supersonic frequency 15-25KHz, ultrasonic power 100-200W, then extracted 60-90 minutes in 60-70 DEG C of water-bath, it extracted
Journey is stirred with 100-300 revs/min, using 300 mesh filter-cloth filterings, obtains oriental wormwood extracting solution, oriental wormwood extracting solution is concentrated under reduced pressure into 65
DEG C when density be 1.05-1.10g/mL clear cream, clear cream is dried at 65 DEG C to constant weight, is crushed, 300 mesh sieve is crossed, obtains oriental wormwood
Extract.
A kind of cancer-resisting Chinese medicine, is prepared using any of the above-described method.
A kind of cancer-resisting Chinese medicine, including following raw materials are prepared：Hairyvein agrimony 20-30g, Radix Astragali 15-25g, Sculellaria barbata
15-25g, oldenlandia diffusa 10-20g, Poria cocos 10-20g, salvia chinensis 8-12g, Chinese violet 8-12g, gynostemma pentaphylla 8-12g, Pu Gong
English 5-10g, Radix Glycyrrhizae 5-10g, Paris polyphylla 5-10g, rainbow conk 5-10g, antistaling agent 0.01-0.1g.
The preparation method of cancer-resisting Chinese medicine of the present invention, obtained cancer-resisting Chinese medicine can effectively enhance immunity of organisms
And muscle power, inhibit growth of cancer cells, prevents cancer cell from spreading and being mutated, and active constituent content is high, is easy to absorb, mitigate cancer
The pain of disease patient.
Specific implementation mode
In the present invention, if not refering in particular to, all devices and raw material is commercially available or the industry is common are following
Method in embodiment is unless otherwise instructed this field conventional method.
Hairyvein agrimony is the dry aerial parts of rosaceous plant hairyvein agrimony Agrimonia pilosa Ledeb..Both it can kill
Hinder cancer cell, and be conducive to normal cell, can also mitigate the injury to body after radiotherapy, chemotherapy.The macro safe prepared slices of Chinese crude drugs in Minyue County
Co., Ltd provides.
Radix Astragali is legume astragalus mongolicus Astragalus membranaceus (Fisch.)
Bge.var.mongholicus (Bge.) Hsiao or Astragalus membranacus Astragalus membranaceus (Fisch.) Bge,
Dry root.Tonifying middle-Jiao and Qi enhances body's immunity.Hong Tai prepared slices of Chinese crude drugs Co., Ltd of Minyue County provides.
Rainbow conk, the dry son for being On Polyporaceae Corilus versicolor Quel. Coriolus versicolor (L.exFr.) Quel
Entity.It is clearing heat and detoxicating with invigorating spleen to remove dampness, it is antitumor and other effects.Anguo City Jin Da local and special products sales department provides.
Sculellaria barbata is labiate Sculellaria barbata Scutellaria barbata Don's [S.rivularis Wall]
Dry herb.Have the function of clearing heat and detoxicating, promoting blood circulation, swelling and pain relieving, anticancer, adjust it is immune etc..Bozhou Bai Chuan medicine companies are limited
Company provides.
Oldenlandia diffusa is Rubiaceae plants of Hedyotis oldenlandia diffusa Hedyotis diffusa Willd.
The herb of [Oldenlandia diffusa (Willd.) Roxb.].With clearing heat and detoxicating, dampness removing disappears carbuncle, anticancer, and enhancing is immune
Function and other effects.Bozhou Bai Chuan pharmaceutcal corporation, Ltds provide.
Paris polyphylla is Liliaceae paris plant China Paris polyphylla Paris polyphylla Smith var.chinenisi
(Franch) Hara, Yunnan Rhizoma Paridis Paris polyphylla Smith var.yunnanensis (Franch.) Hand.-
Or the dry rhizome of paris polyphylla Paris polyphylla Smith var, chinensis (Franch.) Hara Mazz..
With clearing heat and detoxicating, swelling and pain relieving, the effect of cool liver arresting convulsion, anticancer, the growth of tumour can inhibit, moreover it is possible to eliminate tumour and its week
Enclose the inflammation and oedema of tissue.Bozhou Bai Chuan pharmaceutcal corporation, Ltds provide.
Salvia chinensis, Lamiaceae Salvia platymiscium salvia chinensis Salvia chinensia Benth., with all herbal medicine.It can live
Blood stagnation resolvation, detoxicating and resolving a mass.Bozhou Bai Chuan pharmaceutcal corporation, Ltds provide.
Chinese violet is the drying herb of violaceae plant Chinese violet Viola yedoensis Makino.With clear
Thermal detoxification, cool blood detumescence, the effect of clearing heat and promoting diuresis.Often rich medicine company sale Co., Ltd of Haozhou city provides.
Poria cocos, the dry sclerotia of On Polyporaceae Poria cocos Poria cocos (Schw.) Wolf, antitoxic heart-soothing and sedative, Toxin-Vanquishing are anti-
Cancer, antibacterial, contained pachymic acid have strengthen immunity, antitumor and calm and other effects.Bozhou Bai Chuan pharmaceutcal corporation, Ltds carry
For.
Gynostemma pentaphylla, Classification system：Gynostemma pentaphyllum (Thunb.) Makino, all herbal medicine.Mainly have
It is gynosaponin, gynostemma pentaphylla glucoside (polysaccharide), water-soluble amino acids, flavonoids, multivitamin, trace element, mine to imitate into g
Substance etc. has and replenishes qi to invigorate the spleen, clearing heat and detoxicating, improves immunity and other effects.Bozhou Bai Chuan pharmaceutcal corporation, Ltds provide.
Dandelion is feverfew dandelion Taraxacum mongolicum Hand-Mazz., alkali ground dandelion
Taraxacum sinicum Kitag. or the drying herb for belonging to several plants.It is clearing heat and detoxicating, diuresis dissipating bind.Bozhou Bai Chuan medicines
Industry Co., Ltd provides.
Radix Glycyrrhizae is glycyrrhizic legume Glycyrrhiza uralensis Fisch., swollen fruit Radix Glycyrrhiza
The dry root of inflata Bat. or glycyrrhiza glabra Glycyrrhiza glabra L..Can coordinating the drug actions of a prescription, contained hypo acid can hinder
The effect of disconnected Induced by Carcinogen tumour growth.Bozhou Bai Chuan pharmaceutcal corporation, Ltds provide.
Folium isatidis is the dried leaf of cruciferae isatis Isatis indigotica Fort..Bozhou Bai Chuan medicine companies
Co., Ltd provides.
Oriental wormwood, for for feverfew artemisia scoparia Artemisia scoparia Waldst.et Kit. or Artemisia capillaris
The dry aerial parts of Artemisia capillaris Thunb..Prosperous plantation farmer professional is contained using Longnan City Wudu District to close
2 months oriental wormwoods of society's offer are provided.
Glycine, Hunan century, Hua Xing bioengineering Co., Ltd provided.
Serine, Hunan century, Hua Xing bioengineering Co., Ltd provided.
Cellulase, using the cellulase of the model PB33 of Pangbo Bioengineering Co Ltd, Nanning's production, enzyme activity 1
Ten thousand U/g.
Fungistatic effect is tested：The cancer-resisting Chinese medicine opening that embodiment 1-8 is obtained is positioned in Brown Glass Brown glass bottles and jars only, in ring
Border temperature is 30 DEG C, relative humidity is 85% placement 150 days, the total plate count of test staphylococcus aureus (ATCC 6538),
With reference to GB/T 4789.10-2010《Microbiological test of food hygiene Staphylococcus aureus count》Method carry out.
Embodiment 1
The preparation method of cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 8g, rainbow conk 8g are crushed respectively, 40 mesh sieve is crossed, 320g water is added after mixing, 90
DEG C water carries 3 hours, using 300 mesh filter-cloth filterings, obtains filtrate, and filtrate is pressure is 0.02MPa, temperature is 60 DEG C and is concentrated under reduced pressure into
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder by the clear cream that density at 60 DEG C is 1.12g/mL
A；
(2) mixed powder B is prepared：By hairyvein agrimony 25g, Radix Astragali 20g, Sculellaria barbata 20g, oldenlandia diffusa 15g, Poria cocos 15g, stone
See and wear 10g, Chinese violet 10g, gynostemma pentaphylla 10g, dandelion 8g, Radix Glycyrrhizae 8g, crush respectively, cross 40 mesh sieve, mixing is mixed
Powder B；
(3) it digests：The water of 11 times of mixed powder B mass is added in mixed powder B, mixed powder B mass 0.2% is added after mixing
Cellulase is stirred 80 minutes with 150 revs/min at 45 DEG C, obtains mixed liquor C；
(4) water carries：Mixed liquor C is carried 80 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue, filter
The water of 8 times of mixed powder B mass is added in slag, is carried 50 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains liquor B, merges filter
Liquid A and liquor B, obtain liquor C, liquor C pressure be 0.02MPa, temperature be 60 DEG C be concentrated under reduced pressure into 60 DEG C when density be
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by the clear cream of 1.12g/mL；
(5) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.05g are uniformly mixed, cancer-resisting Chinese medicine is obtained.
The antistaling agent is Folium Isatidis extract.
The Folium Isatidis extract is prepared by following methods：Folium isatidis is crushed, 40 mesh sieve is crossed, obtains folium isatidis
Powder mixes the ethanol water that folium isatidis powder 50g and 600mL volume fraction is 65%, in 65 DEG C of refluxing extractions 3 hours, adopts
With 300 mesh filter-cloth filterings, filtrate and filter residue are obtained, the ethanol water that 400mL volume fractions are 65% is added in filter residue,
65 DEG C of refluxing extractions 2 hours merge filtrate twice using 300 mesh filter-cloth filterings, pressure is 0.02MPa, temperature is 65 DEG C and subtracts
Density is 1.08g/mL when pressure is concentrated into 65 DEG C, then is dried to constant weight at 65 DEG C, is crushed, and 300 mesh sieve is crossed, and obtains folium isatidis extraction
Object.
Embodiment 2
The preparation method of cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 8g, rainbow conk 8g are crushed respectively, 40 mesh sieve is crossed, 320g water is added after mixing, 90
DEG C water carries 3 hours, using 300 mesh filter-cloth filterings, obtains filtrate, and filtrate is pressure is 0.02MPa, temperature is 60 DEG C and is concentrated under reduced pressure into
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder by the clear cream that density at 60 DEG C is 1.12g/mL
A；
(2) mixed powder B is prepared：By hairyvein agrimony 25g, Radix Astragali 20g, Sculellaria barbata 20g, oldenlandia diffusa 15g, Poria cocos 15g, stone
See and wear 10g, Chinese violet 10g, gynostemma pentaphylla 10g, dandelion 8g, Radix Glycyrrhizae 8g, crush respectively, cross 40 mesh sieve, mixing is mixed
Powder B；
(3) freeze-thaw is handled：The water of 3 times of mixed powder B mass is added in mixed powder B, is stirred 15 minutes with 80 revs/min,
50 minutes are stood at 25 DEG C, obtains mixed liquor A, mixed liquor A is freezed 2 hours at -40 DEG C, is ultrasonically treated 30 minutes after taking-up,
40 minutes are stood at 30 DEG C again, the mixed liquor A thawed freezes the mixed liquor A of defrosting 2 hours at -40 DEG C, surpasses after taking-up
Sonication 30 minutes, then 40 minutes are stood at 30 DEG C, obtain mixed liquid B；
(4) it digests：The water of 8 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass 0.2% is added after mixing
Cellulase, 45 DEG C with 150 revs/min stir 80 minutes, obtain mixed liquor C；
(5) water carries：Mixed liquor C is carried 80 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue, filter
The water of 8 times of mixed powder B mass is added in slag, is carried 50 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains liquor B, merges filter
Liquid A and liquor B, obtain liquor C, liquor C pressure be 0.02MPa, temperature be 60 DEG C be concentrated under reduced pressure into 60 DEG C when density be
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by the clear cream of 1.12g/mL；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.05g are uniformly mixed, cancer-resisting Chinese medicine is obtained.
The operating condition of step (3) described supersound process is 60 DEG C, ultrasonic power 200W of ultrasonic temperature, supersonic frequency
20KHz。
The antistaling agent is Folium Isatidis extract.
The Folium Isatidis extract is prepared by following methods：Folium isatidis is crushed, 40 mesh sieve is crossed, obtains folium isatidis
Powder mixes the ethanol water that folium isatidis powder 50g and 600mL volume fraction is 65%, in 65 DEG C of refluxing extractions 3 hours, adopts
With 300 mesh filter-cloth filterings, filtrate and filter residue are obtained, the ethanol water that 400mL volume fractions are 65% is added in filter residue,
65 DEG C of refluxing extractions 2 hours merge filtrate twice using 300 mesh filter-cloth filterings, pressure is 0.02MPa, temperature is 65 DEG C and subtracts
Density when pressure is concentrated into 65 DEG C is 1.08g/mL, then is dried to constant weight at 65 DEG C, is crushed, and crosses 300 mesh sieve, obtains folium isatidis and carry
Take object.
Embodiment 3
The preparation method of cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 8g, rainbow conk 8g are crushed respectively, 40 mesh sieve is crossed, 320g water is added after mixing, 90
DEG C water carries 3 hours, using 300 mesh filter-cloth filterings, obtains filtrate, and filtrate is pressure is 0.02MPa, temperature is 60 DEG C and is concentrated under reduced pressure into
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder by the clear cream that density at 60 DEG C is 1.12g/mL
A；
(2) mixed powder B is prepared：By hairyvein agrimony 25g, Radix Astragali 20g, Sculellaria barbata 20g, oldenlandia diffusa 15g, Poria cocos 15g, stone
See and wear 10g, Chinese violet 10g, gynostemma pentaphylla 10g, dandelion 8g, Radix Glycyrrhizae 8g, crush respectively, cross 40 mesh sieve, mixing is mixed
Powder B；
(3) freeze-thaw is handled：The water of 3 times of mixed powder B mass is added in mixed powder B, is stirred 15 minutes with 80 revs/min,
50 minutes are stood at 25 DEG C, obtains mixed liquor A, mixed liquor A is freezed 2 hours at -40 DEG C, is ultrasonically treated 30 minutes after taking-up,
40 minutes are stood at 30 DEG C again, the mixed liquor A thawed freezes the mixed liquor A of defrosting 2 hours at -40 DEG C, surpasses after taking-up
Sonication 30 minutes, then 40 minutes are stood at 30 DEG C, obtain mixed liquid B；
(4) it digests：The water of 8 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass 0.2% is added after mixing
Cellulase and 0.3% enzymolysis auxiliary agent, 45 DEG C with 150 revs/min stir 80 minutes, obtain mixed liquor C；
(5) water carries：Mixed liquor C is carried 80 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue, filter
The water of 8 times of mixed powder B mass is added in slag, is carried 50 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains liquor B, merges filter
Liquid A and liquor B, obtain liquor C, liquor C pressure be 0.02MPa, temperature be 60 DEG C be concentrated under reduced pressure into 60 DEG C when density be
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by the clear cream of 1.12g/mL；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.05g are uniformly mixed, cancer-resisting Chinese medicine is obtained.
The operating condition of the step (3) supersound process is 60 DEG C, ultrasonic power 200W of ultrasonic temperature, supersonic frequency
20KHz。
The enzymolysis auxiliary agent is glycine.
The antistaling agent is Folium Isatidis extract.
The Folium Isatidis extract is prepared by following methods：Folium isatidis is crushed, 40 mesh sieve is crossed, obtains folium isatidis
Powder mixes the ethanol water that folium isatidis powder 50g and 600mL volume fraction is 65%, in 65 DEG C of refluxing extractions 3 hours, adopts
With 300 mesh filter-cloth filterings, filtrate and filter residue are obtained, the ethanol water that 400mL volume fractions are 65% is added in filter residue,
65 DEG C of refluxing extractions 2 hours merge filtrate twice using 300 mesh filter-cloth filterings, pressure is 0.02MPa, temperature is 65 DEG C and subtracts
Density is 1.08g/mL when pressure is concentrated into 65 DEG C, then is dried to constant weight at 65 DEG C, is crushed, and 300 mesh sieve is crossed, and obtains folium isatidis extraction
Object.
Embodiment 4
The preparation method of cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 8g, rainbow conk 8g are crushed respectively, 40 mesh sieve is crossed, 320g water is added after mixing, 90
DEG C water carries 3 hours, using 300 mesh filter-cloth filterings, obtains filtrate, and filtrate is pressure is 0.02MPa, temperature is 60 DEG C and is concentrated under reduced pressure into
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder by the clear cream that density at 60 DEG C is 1.12g/mL
A；
(2) mixed powder B is prepared：By hairyvein agrimony 25g, Radix Astragali 20g, Sculellaria barbata 20g, oldenlandia diffusa 15g, Poria cocos 15g, stone
See and wear 10g, Chinese violet 10g, gynostemma pentaphylla 10g, dandelion 8g, Radix Glycyrrhizae 8g, crush respectively, cross 40 mesh sieve, mixing is mixed
Powder B；
(3) freeze-thaw is handled：The water of 3 times of mixed powder B mass is added in mixed powder B, is stirred 15 minutes with 80 revs/min,
50 minutes are stood at 25 DEG C, obtains mixed liquor A, mixed liquor A is freezed 2 hours at -40 DEG C, is ultrasonically treated 30 minutes after taking-up,
40 minutes are stood at 30 DEG C again, the mixed liquor A thawed freezes the mixed liquor A of defrosting 2 hours at -40 DEG C, surpasses after taking-up
Sonication 30 minutes, then 40 minutes are stood at 30 DEG C, obtain mixed liquid B；
(4) it digests：The water of 8 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass 0.2% is added after mixing
Cellulase and 0.3% enzymolysis auxiliary agent, 45 DEG C with 150 revs/min stir 80 minutes, obtain mixed liquor C；
(5) water carries：Mixed liquor C is carried 80 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue, filter
The water of 8 times of mixed powder B mass is added in slag, is carried 50 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains liquor B, merges filter
Liquid A and liquor B, obtain liquor C, liquor C pressure be 0.02MPa, temperature be 60 DEG C be concentrated under reduced pressure into 60 DEG C when density be
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by the clear cream of 1.12g/mL；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.05g are uniformly mixed, cancer-resisting Chinese medicine is obtained.
The operating condition of step (3) described supersound process is 60 DEG C, ultrasonic power 200W of ultrasonic temperature, supersonic frequency
20KHz。
The enzymolysis auxiliary agent is Serine.
The antistaling agent is Folium Isatidis extract.
The Folium Isatidis extract is prepared by following methods：Folium isatidis is crushed, 40 mesh sieve is crossed, obtains folium isatidis
Powder mixes the ethanol water that folium isatidis powder 50g and 600mL volume fraction is 65%, in 65 DEG C of refluxing extractions 3 hours, adopts
With 300 mesh filter-cloth filterings, filtrate and filter residue are obtained, the ethanol water that 400mL volume fractions are 65% is added in filter residue,
65 DEG C of refluxing extractions 2 hours merge filtrate twice using 300 mesh filter-cloth filterings, pressure is 0.02MPa, temperature is 65 DEG C and subtracts
Density is 1.08g/mL when pressure is concentrated into 65 DEG C, then is dried to constant weight at 65 DEG C, is crushed, and 300 mesh sieve is crossed, and obtains folium isatidis extraction
Object.
Embodiment 5
The preparation method of cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 8g, rainbow conk 8g are crushed respectively, 40 mesh sieve is crossed, 320g water is added after mixing, 90
DEG C water carries 3 hours, using 300 mesh filter-cloth filterings, obtains filtrate, and filtrate is pressure is 0.02MPa, temperature is 60 DEG C and is concentrated under reduced pressure into
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder by the clear cream that density at 60 DEG C is 1.12g/mL
A；
(2) mixed powder B is prepared：By hairyvein agrimony 25g, Radix Astragali 20g, Sculellaria barbata 20g, oldenlandia diffusa 15g, Poria cocos 15g, stone
See and wear 10g, Chinese violet 10g, gynostemma pentaphylla 10g, dandelion 8g, Radix Glycyrrhizae 8g, crush respectively, cross 40 mesh sieve, mixing is mixed
Powder B；
(3) freeze-thaw is handled：The water of 3 times of mixed powder B mass is added in mixed powder B, is stirred 15 minutes with 80 revs/min,
50 minutes are stood at 25 DEG C, obtains mixed liquor A, mixed liquor A is freezed 2 hours at -40 DEG C, is ultrasonically treated 30 minutes after taking-up,
40 minutes are stood at 30 DEG C again, the mixed liquor A thawed freezes the mixed liquor A of defrosting 2 hours at -40 DEG C, surpasses after taking-up
Sonication 30 minutes, then 40 minutes are stood at 30 DEG C, obtain mixed liquid B；
(4) it digests：The water of 8 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass 0.2% is added after mixing
Cellulase and 0.3% enzymolysis auxiliary agent, 45 DEG C with 150 revs/min stir 80 minutes, obtain mixed liquor C；
(5) water carries：Mixed liquor C is carried 80 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue, filter
The water of 8 times of mixed powder B mass is added in slag, is carried 50 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains liquor B, merges filter
Liquid A and liquor B, obtain liquor C, liquor C pressure be 0.02MPa, temperature be 60 DEG C be concentrated under reduced pressure into 60 DEG C when density be
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by the clear cream of 1.12g/mL；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.05g are uniformly mixed, cancer-resisting Chinese medicine is obtained.
The operating condition of step (3) described supersound process is 60 DEG C, ultrasonic power 200W of ultrasonic temperature, supersonic frequency
20KHz。
The enzymolysis auxiliary agent is the mixture of the glycine of 30wt% and the Serine of 70wt%.
The antistaling agent is Folium Isatidis extract.
The Folium Isatidis extract is prepared by following methods：Folium isatidis is crushed, 40 mesh sieve is crossed, obtains folium isatidis
Powder mixes the ethanol water that folium isatidis powder 50g and 600mL volume fraction is 65%, in 65 DEG C of refluxing extractions 3 hours, adopts
With 300 mesh filter-cloth filterings, filtrate and filter residue are obtained, the ethanol water that 400mL volume fractions are 65% is added in filter residue,
65 DEG C of refluxing extractions 2 hours merge filtrate twice using 300 mesh filter-cloth filterings, pressure is 0.02MPa, temperature is 65 DEG C and subtracts
Density is 1.08g/mL when pressure is concentrated into 65 DEG C, then is dried to constant weight at 65 DEG C, is crushed, and 300 mesh sieve is crossed, and obtains folium isatidis extraction
Object.
Embodiment 6
The preparation method of cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 8g, rainbow conk 8g are crushed respectively, 40 mesh sieve is crossed, 320g water is added after mixing, 90
DEG C water carries 3 hours, using 300 mesh filter-cloth filterings, obtains filtrate, and filtrate is pressure is 0.02MPa, temperature is 60 DEG C and is concentrated under reduced pressure into
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder by the clear cream that density at 60 DEG C is 1.12g/mL
A；
(2) mixed powder B is prepared：By hairyvein agrimony 25g, Radix Astragali 20g, Sculellaria barbata 20g, oldenlandia diffusa 15g, Poria cocos 15g, stone
See and wear 10g, Chinese violet 10g, gynostemma pentaphylla 10g, dandelion 8g, Radix Glycyrrhizae 8g, crush respectively, cross 40 mesh sieve, mixing is mixed
Powder B；
(3) freeze-thaw is handled：The water of 3 times of mixed powder B mass is added in mixed powder B, is stirred 15 minutes with 80 revs/min,
50 minutes are stood at 25 DEG C, obtains mixed liquor A, mixed liquor A is freezed 2 hours at -40 DEG C, is ultrasonically treated 30 minutes after taking-up,
40 minutes are stood at 30 DEG C again, the mixed liquor A thawed freezes the mixed liquor A of defrosting 2 hours at -40 DEG C, surpasses after taking-up
Sonication 30 minutes, then 40 minutes are stood at 30 DEG C, obtain mixed liquid B；
(4) it digests：The water of 8 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass 0.2% is added after mixing
Cellulase and 0.3% enzymolysis auxiliary agent, 45 DEG C with 150 revs/min stir 80 minutes, obtain mixed liquor C；
(5) water carries：Mixed liquor C is carried 80 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue, filter
The water of 8 times of mixed powder B mass is added in slag, is carried 50 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains liquor B, merges filter
Liquid A and liquor B, obtain liquor C, liquor C pressure be 0.02MPa, temperature be 60 DEG C be concentrated under reduced pressure into 60 DEG C when density be
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by the clear cream of 1.12g/mL；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.05g are uniformly mixed, cancer-resisting Chinese medicine is obtained.
The operating condition of step (3) described supersound process is 60 DEG C, ultrasonic power 200W of ultrasonic temperature, supersonic frequency
20KHz。
The enzymolysis auxiliary agent is the mixture of the glycine of 30wt% and the Serine of 70wt%.
The antistaling agent is capillary extract.
The capillary extract is prepared by following methods：Oriental wormwood is crushed, 40 mesh sieve is crossed, oriental wormwood powder is obtained, by mattress
Old powder 50g and the mixing of 600mL water, are ultrasonically treated 15 minutes, supersonic frequency 20KHz, ultrasonic power 150W at 60-70 DEG C, then
65 DEG C of water-baths are extracted 80 minutes, and extraction process is stirred with 200 revs/min, using 300 mesh filter-cloth filterings, obtains oriental wormwood extracting solution, mattress
Old extracting solution pressure be 0.02MPa, temperature be 65 DEG C be concentrated under reduced pressure into 65 DEG C when density be 1.08g/mL clear cream, by clear cream
It is dried at 65 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain capillary extract.
Embodiment 7
It is substantially the same manner as Example 6, it differs only in：The capillary extract is prepared by following methods：By oriental wormwood
It crushes, crosses 40 mesh sieve, obtain oriental wormwood powder, oriental wormwood powder 50g and 600mL water is mixed, 1.0g sodium bicarbonates are added, at 60-70 DEG C
Be ultrasonically treated 15 minutes, supersonic frequency 20KHz, ultrasonic power 150W, then extracted 80 minutes in 65 DEG C of water-baths, extraction process with
200 revs/min of stirrings obtain oriental wormwood extracting solution using 300 mesh filter-cloth filterings, and oriental wormwood extracting solution is 0.02MPa, temperature in pressure
Clear cream is dried at 65 DEG C to constant weight, crushes, cross 300 by the clear cream that density is 1.08g/mL when being concentrated under reduced pressure into 65 DEG C for 65 DEG C
Mesh sieves, and obtains capillary extract.
Embodiment 8
The preparation method of cancer-resisting Chinese medicine, includes the following steps：
(1) mixed powder A is prepared：Paris polyphylla 8g, rainbow conk 8g are crushed respectively, 40 mesh sieve is crossed, 320g water is added after mixing, 90
DEG C water carries 3 hours, using 300 mesh filter-cloth filterings, obtains filtrate, and filtrate is pressure is 0.02MPa, temperature is 60 DEG C and is concentrated under reduced pressure into
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder by the clear cream that density at 60 DEG C is 1.12g/mL
A；
(2) mixed powder B is prepared：By hairyvein agrimony 25g, Radix Astragali 20g, Sculellaria barbata 20g, oldenlandia diffusa 15g, Poria cocos 15g, stone
See and wear 10g, Chinese violet 10g, gynostemma pentaphylla 10g, dandelion 8g, Radix Glycyrrhizae 8g, crush respectively, cross 40 mesh sieve, mixing is mixed
Powder B；
(3) freeze-thaw is handled：The water of 3 times of mixed powder B mass is added in mixed powder B, is stirred 15 minutes with 80 revs/min,
50 minutes are stood at 25 DEG C, obtains mixed liquor A, mixed liquor A is freezed 2 hours at -40 DEG C, is ultrasonically treated 30 minutes after taking-up,
40 minutes are stood at 30 DEG C again, the mixed liquor A thawed freezes the mixed liquor A of defrosting 2 hours at -40 DEG C, surpasses after taking-up
Sonication 30 minutes, then 40 minutes are stood at 30 DEG C, obtain mixed liquid B；
(4) it digests：The water of 8 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass 0.2% is added after mixing
Cellulase and 0.3% enzymolysis auxiliary agent, 45 DEG C with 150 revs/min stir 80 minutes, obtain mixed liquor C；
(5) water carries：Mixed liquor C is carried 80 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue, filter
The water of 8 times of mixed powder B mass is added in slag, is carried 50 minutes in 96 DEG C of water, using 300 mesh filter-cloth filterings, obtains liquor B, merges filter
Liquid A and liquor B, obtain liquor C, liquor C pressure be 0.02MPa, temperature be 60 DEG C be concentrated under reduced pressure into 60 DEG C when density be
Clear cream is dried at 60 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by the clear cream of 1.12g/mL；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.05g are uniformly mixed, cancer-resisting Chinese medicine is obtained.
The operating condition of step (3) described supersound process is 60 DEG C, ultrasonic power 200W of ultrasonic temperature, supersonic frequency
20KHz。
The enzymolysis auxiliary agent is the mixture of the glycine of 30wt% and the Serine of 70wt%.
The antistaling agent is the mixture of 25wt% Folium Isatidis extracts and 75wt% capillary extracts.
The Folium Isatidis extract is prepared by following methods：Folium isatidis is crushed, 40 mesh sieve is crossed, obtains folium isatidis
Powder mixes the ethanol water that folium isatidis powder 50g and 600mL volume fraction is 65%, in 65 DEG C of refluxing extractions 3 hours, adopts
With 300 mesh filter-cloth filterings, filtrate and filter residue are obtained, the ethanol water that 400mL volume fractions are 65% is added in filter residue,
65 DEG C of refluxing extractions 2 hours merge filtrate twice using 300 mesh filter-cloth filterings, pressure is 0.02MPa, temperature is 65 DEG C and subtracts
Density is 1.08g/mL when pressure is concentrated into 65 DEG C, then is dried to constant weight at 65 DEG C, is crushed, and 300 mesh sieve is crossed, and obtains folium isatidis extraction
Object.
The capillary extract is prepared by following methods：Oriental wormwood is crushed, 40 mesh sieve is crossed, oriental wormwood powder is obtained, by mattress
Old powder 50g and the mixing of 600mL water, are added 1.0g sodium bicarbonates, are ultrasonically treated 15 minutes, supersonic frequency 20KHz at 60-70 DEG C,
Ultrasonic power 150W, then extracted 80 minutes in 65 DEG C of water-baths, extraction process is stirred with 200 revs/min, using 300 mesh filter-cloth filterings,
Obtain oriental wormwood extracting solution, oriental wormwood extracting solution pressure be 0.02MPa, temperature be 65 DEG C be concentrated under reduced pressure into 65 DEG C when density be
Clear cream is dried at 65 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain capillary extract by the clear cream of 1.08g/mL.
Test case 1
The cancer-resisting Chinese medicine that embodiment 1-8 is obtained is respectively with water by solid-to-liquid ratio 1g：100mL is uniformly mixed as experiment
Group 1-8 by inspection drug, take BALB/C mice 135, be randomly divided into 9 groups, every group 15, respectively as experimental group 1-8 and sky
White control group, specific experiment method with reference to just chat about, Shi Jianguo etc.《When micron Chinese medicine compound anti-cancer No.1 extends He tumor mouse survivals
Between mechanism》, Chinese Clinical rehabilitation, to tumor inhibitory effect, statistics is each for the method test of 1.4.2.2 in 2003.7 (8)
The average weight of group object tumor, P between each group<0.05, difference has statistical significance.Calculate the tumor control rate of each group.Tool
Body examination test result is shown in Table 1.
1 cancer-resisting Chinese medicine tumor inhibitory effect test result table of table
| Tumor control rate (%) | |
| Embodiment 1 | 29.12 |
| Embodiment 2 | 32.34 |
| Embodiment 3 | 34.87 |
| Embodiment 4 | 36.75 |
| Embodiment 5 | 40.92 |
| Embodiment 6 | 38.66 |
| Embodiment 7 | 43.24 |
| Embodiment 8 | 47.85 |
Test case 2
The anti-corrosion effect of the embodiment 1-8 cancer-resisting Chinese medicines being prepared is tested, specific test result is shown in Table
2。
2 fungistatic effect test result table of table
| S. aureus colonies sum, cfu/g | |
| Embodiment 1 | 761 |
| Embodiment 2 | 684 |
| Embodiment 3 | 591 |
| Embodiment 4 | 546 |
| Embodiment 5 | 443 |
| Embodiment 6 | 486 |
| Embodiment 7 | 402 |
| Embodiment 8 | 297 |
Embodiment 2 handles mixed powder B, tumor control rate and the anti-corrosion effect of obtained cancer-resisting Chinese medicine using freeze-thaw
Fruit is better than embodiment 1.It is freezed after Chinese medicine water suction, sharp ice crystal can be formed in Chinese medicine, ice crystal punctures cell wall and is conducive to
Active ingredient is overflowed, to obtain more active ingredients.Embodiment 3 adds enzymolysis auxiliary agent, tumor control rate and anti-corrosion effect
Fruit is better than embodiment 2.Cell wall can be destroyed by changes such as hydrogen bond, Van der Waals force, electrostatic attractions by digesting auxiliary agent, be conducive to
Ingredient is imitated to overflow.
Claims (8)
1. a kind of preparation method of cancer-resisting Chinese medicine, which is characterized in that include the following steps：
(1) mixed powder A is prepared：Paris polyphylla 5-10g, rainbow conk 5-10g are crushed respectively, 40 mesh sieve is crossed, 200-400g is added after mixing
Water carries 2-4 hours in 85-95 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate, density when filtrate decompression is concentrated into 60 DEG C is
Clear cream is dried at 50-70 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder A by the clear cream of 1.10-1.15g/mL；
(2) mixed powder B is prepared：By hairyvein agrimony 20-30g, Radix Astragali 15-25g, Sculellaria barbata 15-25g, oldenlandia diffusa 10-20g, Fu
Siberian cocklebur 10-20g, salvia chinensis 8-12g, Chinese violet 8-12g, gynostemma pentaphylla 8-12g, dandelion 5-10g, Radix Glycyrrhizae 5-10g, difference powder
It is broken, 40 mesh sieve is crossed, mixing obtains mixed powder B；
(3) it digests：The water of 7-14 times of mixed powder B mass is added in mixed powder B, mixed powder B mass 0.1-0.3% is added after mixing
Cellulase, 40-50 DEG C with 100-200 revs/min stir 60-90 minutes, obtain mixed liquor C；
(4) water carries：Mixed liquor C is carried 60-90 minutes in 90-98 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue,
The water of 6-10 times of mixed powder B mass is added in filter residue, carries 40-60 minutes in 90-98 DEG C of water, using 300 mesh filter-cloth filterings, must filter
Liquid B, merging filtrate A and liquor B, obtain liquor C, and density when liquor C is concentrated under reduced pressure into 60 DEG C is the clear of 1.10-1.15g/mL
Clear cream is dried at 50-70 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by cream；
(5) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.01-0.1g are mixed, routinely technique preparation, is prevented
Cancer Chinese herbal medicine for preventing.
2. a kind of preparation method of cancer-resisting Chinese medicine, which is characterized in that include the following steps：
(1) mixed powder A is prepared：Paris polyphylla 5-10g, rainbow conk 5-10g are crushed respectively, 40 mesh sieve is crossed, 200-400g is added after mixing
Water carries 2-4 hours in 85-95 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate, density when filtrate decompression is concentrated into 60 DEG C is
Clear cream is dried at 50-70 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder A by the clear cream of 1.10-1.15g/mL；
(2) mixed powder B is prepared：By hairyvein agrimony 20-30g, Radix Astragali 15-25g, Sculellaria barbata 15-25g, oldenlandia diffusa 10-20g, Fu
Siberian cocklebur 10-20g, salvia chinensis 8-12g, Chinese violet 8-12g, gynostemma pentaphylla 8-12g, dandelion 5-10g, Radix Glycyrrhizae 5-10g, difference powder
It is broken, 40 mesh sieve is crossed, mixing obtains mixed powder B；
(3) freeze-thaw is handled：The water of 2-4 times of mixed powder B mass is added in mixed powder B, with 60-100 revs/min of stirring 10-20
Minute, 30-60 minutes are stood at 20-30 DEG C, obtains mixed liquor A, mixed liquor A is freezed 1-3 hours at -60~-20 DEG C, is taken out
It is ultrasonically treated 20-40 minutes afterwards, then 30-50 minutes is stood at 25-35 DEG C, the mixed liquor to be thawed, by the mixed liquor of defrosting
It carries out freeze-thaw processing again under these conditions, obtains mixed liquid B；
(4) it digests：The water of 5-10 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass 0.1- is added after mixing
0.3% cellulase is stirred 60-90 minutes with 100-200 revs/min at 40-50 DEG C, obtains mixed liquor C；
(5) water carries：Mixed liquor C is carried 60-90 minutes in 90-98 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue,
The water of 6-10 times of mixed powder B mass is added in filter residue, carries 40-60 minutes in 90-98 DEG C of water, using 300 mesh filter-cloth filterings, must filter
Liquid B, merging filtrate A and liquor B, obtain liquor C, and density when liquor C is concentrated under reduced pressure into 60 DEG C is the clear of 1.10-1.15g/mL
Clear cream is dried at 50-70 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by cream；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.01-0.1g are mixed, routinely technique preparation, is prevented
Cancer Chinese herbal medicine for preventing.
3. a kind of preparation method of cancer-resisting Chinese medicine, which is characterized in that include the following steps：
(1) mixed powder A is prepared：Paris polyphylla 5-10g, rainbow conk 5-10g are crushed respectively, 40 mesh sieve is crossed, 200-400g is added after mixing
Water carries 2-4 hours in 85-95 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate, density when filtrate decompression is concentrated into 60 DEG C is
Clear cream is dried at 50-70 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder A by the clear cream of 1.10-1.15g/mL；
(2) mixed powder B is prepared：By hairyvein agrimony 20-30g, Radix Astragali 15-25g, Sculellaria barbata 15-25g, oldenlandia diffusa 10-20g, Fu
Siberian cocklebur 10-20g, salvia chinensis 8-12g, Chinese violet 8-12g, gynostemma pentaphylla 8-12g, dandelion 5-10g, Radix Glycyrrhizae 5-10g, difference powder
It is broken, 40 mesh sieve is crossed, mixing obtains mixed powder B；
(3) freeze-thaw is handled：The water of 2-4 times of mixed powder B mass is added in mixed powder B, with 60-100 revs/min of stirring 10-20
Minute, 30-60 minutes are stood at 20-30 DEG C, obtains mixed liquor A, mixed liquor A is freezed 1-3 hours at -60~-20 DEG C, is taken out
It is ultrasonically treated 20-40 minutes afterwards, then 30-50 minutes is stood at 25-35 DEG C, the mixed liquor to be thawed, by the mixed liquor of defrosting
It carries out freeze-thaw processing again under these conditions, obtains mixed liquid B；
(4) it digests：The water of 5-10 times of mixed powder B mass is added in mixed liquid B, and mixed powder B mass 0.1- is added after mixing
0.3% cellulase and the enzymolysis auxiliary agent of 0.1-0.5% are stirred 60-90 minutes with 100-200 revs/min at 40-50 DEG C, are obtained
To mixed liquor C；
(5) water carries：Mixed liquor C is carried 60-90 minutes in 90-98 DEG C of water, using 300 mesh filter-cloth filterings, obtains filtrate A and filter residue,
The water of 6-10 times of mixed powder B mass is added in filter residue, carries 40-60 minutes in 90-98 DEG C of water, using 300 mesh filter-cloth filterings, must filter
Liquid B, merging filtrate A and liquor B, obtain liquor C, and density when liquor C is concentrated under reduced pressure into 60 DEG C is the clear of 1.10-1.15g/mL
Clear cream is dried at 50-70 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain mixed powder C by cream；
(6) mixing, preparation：Mixed powder A, mixed powder C and antistaling agent 0.01-0.1g are uniformly mixed, cancer-resisting Chinese medicine is obtained.
4. the preparation method of cancer-resisting Chinese medicine as claimed in claim 2 or claim 3, which is characterized in that the step (3) is described super
The operating condition of sonication is 50-70 DEG C of ultrasonic temperature, ultrasonic power 100-300W, supersonic frequency 15-25KHz.
5. the preparation method of cancer-resisting Chinese medicine as claimed in claim 3, which is characterized in that the enzymolysis auxiliary agent is sweet ammonia
One or both of acid, Serine mixture.
6. the preparation method of cancer-resisting Chinese medicine as claimed in any one of claims 1-3, which is characterized in that the antistaling agent
For one or both of Folium Isatidis extract and capillary extract mixture.
7. the preparation method of cancer-resisting Chinese medicine as claimed in claim 6, which is characterized in that the capillary extract is by following
Method is prepared：Oriental wormwood is crushed, 40 mesh sieve is crossed, obtains oriental wormwood powder, oriental wormwood powder 40-60g and 500-1000mL water is mixed,
It is ultrasonically treated 10-20 minutes, supersonic frequency 15-25KHz, ultrasonic power 100-200W at 60-70 DEG C, then in 60-70 DEG C of water-bath
Extraction 60-90 minutes, extraction process are stirred with 100-300 revs/min, using 300 mesh filter-cloth filterings, obtain oriental wormwood extracting solution, mattress
Density is the clear cream of 1.05-1.10g/mL when old extracting solution is concentrated under reduced pressure into 65 DEG C, and clear cream is dried at 65 DEG C to constant weight, powder
It is broken, 300 mesh sieve is crossed, capillary extract is obtained.
8. the preparation method of cancer-resisting Chinese medicine as claimed in claim 6, which is characterized in that the capillary extract is by following
Method is prepared：Oriental wormwood is crushed, 40 mesh sieve is crossed, obtains oriental wormwood powder, oriental wormwood powder 40-60g and 500-1000mL water is mixed,
0.8-1.2g sodium bicarbonates are added, are ultrasonically treated 10-20 minutes, supersonic frequency 15-25KHz, ultrasonic power 100- at 60-70 DEG C
200W, then extracted 60-90 minutes in 60-70 DEG C of water-bath, extraction process is stirred with 100-300 revs/min, using 300 mesh filter cloth mistakes
Filter, obtains oriental wormwood extracting solution, and density is the clear cream of 1.05-1.10g/mL when oriental wormwood extracting solution is concentrated under reduced pressure into 65 DEG C, by clear cream
It is dried at 65 DEG C to constant weight, is crushed, crossed 300 mesh sieve, obtain capillary extract.
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| CN110101784A (en) * | 2019-05-22 | 2019-08-09 | 孟繁华 | The preparation method of targeted inhibition lung cancer and its active compound of stem cells hyperplasia, transfer |
| CN112168882A (en) * | 2019-07-02 | 2021-01-05 | 邹国光 | Cancer prevention, anti-cancer and toxin dispelling powder and preparation method thereof |
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| CN112168882A (en) * | 2019-07-02 | 2021-01-05 | 邹国光 | Cancer prevention, anti-cancer and toxin dispelling powder and preparation method thereof |
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