CN108419809A - The application of the anti-root-knot nematode of Chinese medicine composition of the object of bionic extraction containing Momordica grosvenori root - Google Patents

The application of the anti-root-knot nematode of Chinese medicine composition of the object of bionic extraction containing Momordica grosvenori root Download PDF

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Publication number
CN108419809A
CN108419809A CN201810211464.5A CN201810211464A CN108419809A CN 108419809 A CN108419809 A CN 108419809A CN 201810211464 A CN201810211464 A CN 201810211464A CN 108419809 A CN108419809 A CN 108419809A
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China
Prior art keywords
root
momordica grosvenori
bionic extraction
parts
chinese medicine
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CN201810211464.5A
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赵立春
扈芷怡
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Guangxi University of Chinese Medicine
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Guangxi University of Chinese Medicine
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Priority to CN201810211464.5A priority Critical patent/CN108419809A/en
Publication of CN108419809A publication Critical patent/CN108419809A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06Aluminium; Calcium; Magnesium; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/14Celastraceae [Staff-tree or Bittersweet family], e.g. spindle tree, bittersweet or thunder god vine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/20Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia

Abstract

The present invention proposes a kind of application of the anti-root-knot nematode of Chinese medicine composition of the object of bionic extraction containing Momordica grosvenori root, a kind of Chinese medicine composition of the object of bionic extraction containing Momordica grosvenori root, and composition is prepared by the raw material of following parts by weight:Momordica grosvenori root bionic extraction object, derris root, tripterygium wilfordii, quick lime;A method of prevention Meloidogyne incognita, which is characterized in that by the traditional Chinese medicine particle of the object of bionic extraction containing Momordica grosvenori root or liquid and water according to weight ratio 1:10 are configured to liquid, to Siraitia grosvenorii are sprayed or are poured for 80g/ mus by effective ingredient consumption, daily dispenser 1 time, continuous dispenser 7 days.Chinese medicine composition of the present invention has the effect of apparent anti-root-knot nematode, and method safely, effectively, environmental-friendly is provided for the prevention of root-knot nematode.

Description

The application of the anti-root-knot nematode of Chinese medicine composition of the object of bionic extraction containing Momordica grosvenori root
Technical field
The present invention relates to agricultural technology fields, and in particular to a kind of Chinese medicine composition of anti-root-knot nematode and its application.
Background technology
Nematode belongs to Nemathelminthes, is a kind of more low animal, and type is various.Nematode live in soil, seawater and In fresh water, also parasitizes on people, animal and plant, cause disease.Parasitize the referred to as pathogenic for causing disease on plant Nematode or plant nematode.Root-knot nematode is a kind of omnivorousness plant pathogeny line insect of height specialized form.Root-knot nematode host Range is very wide, more than 2000 kinds plants, including monocotyledon, dicotyledon, woody plant and xylophyta.They give agriculture Loss accounts for 90% or more caused by crop, belongs to worldwide distribution.Commonly prevention nematode method includes crop rotation diseases prevention, kills a few days ago Nematode medicament and Variety resistant to SCN are cultivated., wherein shift of crops diseases prevention is widely used, but has certain limitation.And kill line The a large amount of of worm medicament hold high expense using not only expense but also cause considerable degree of pollution.
Siraitia grosvenorii, the fruit of the perennial liana of Curcurbitaceae, alias sweating fruit, balloonvine heartseed herb are described as " angle by people Fruit " is one of the medicine-food two-purpose material that country ratifies in the first batch, and primary efficacy is can be relieving cough and reducing sputum.People are to Siraitia grosvenorii before Developmental research, be limited only to its fruit extraction separation and application, do not consider the development and utilization of its root.Siraitia grosvenorii root fertilizer Greatly, bitter, cold nature have clearing heat and expelling damp, remove obstruction in channels to relieve pain, is anti-inflammatory, solution pain, dropping the effects that enzyme, and clinic proves that it closes rheumatic Section is scorching significant in efficacy.Find there is apparent prevention to make root-knot nematode to the application study of Momordica grosvenori root bionic extraction object With.
Invention content
In order to solve the above technical problems, a kind of Momordica grosvenori root bionic extraction object of present invention offer and its preparation and drop blood The application of fat, and a kind of Chinese medicine composition of reducing blood lipid is provided, apparent effect for reducing blood fat can be effectively played.
To realize above-mentioned technical problem, the present invention provides the following technical solutions:
A kind of Chinese medicine composition of the object of bionic extraction containing Momordica grosvenori root, composition by following parts by weight raw material prepare and At:10-15 parts of Momordica grosvenori root bionic extraction object, 10-15 parts of derris root, 5-10 parts of tripterygium wilfordii, 2-5 parts of quick lime.
As further improvement of the invention, composition is prepared by the raw material of following parts by weight:Momordica grosvenori root is bionical 13 parts of extract, 12 parts of derris root, 7 parts of tripterygium wilfordii, 2 parts of quick lime.
As further improvement of the invention, the preparation method of Momordica grosvenori root bionic extraction object, steps are as follows:
(1) Momordica grosvenori root dried, crushed, sieved with 100 mesh sieve, obtain Momordica grosvenori root powder;
(2) according to Momordica grosvenori root and bionic extraction liquid weight ratio 1:30 ratio mixing, 37 DEG C of heating water baths, plastic film Sealing, magneton extract 120min, and filtrate is collected in filtering, and water-bath is concentrated into 1/4 volume, collects concentrate;
(3) by the concentrate in step (2) according to volume ratio 1:4 mix with ethyl acetate acetic acid butyl acetate solution, extraction 3 It is secondary, organic phase is collected, vacuum rotary steam removes solvent, obtains white powder, as Momordica grosvenori root extract.
Improved as of the invention further, bionic extraction liquid by 0.2% sodium chloride, 0.32% pepsin and 0.02mol/L hydrochloric acid is formulated.
As further improvement of the invention, bionic extraction liquid pH is 4~6.
It is improved as of the invention further, composition can be prepared into granule, dense according to known pharmaceutical technology (tcd) Contracting liquid.
The invention also includes the Chinese medicine composition of the above-mentioned object of bionic extraction containing Momordica grosvenori root is being prevented Momordica grosvenori root tie lines Application in worm.
The invention also includes a kind of methods of prevention Meloidogyne incognita, by the traditional Chinese medicine particle of above-mentioned preparation or Liquid and water are according to weight ratio 1:1-1:20 are configured to liquid, to Siraitia grosvenorii are sprayed for 50-100g/ mus by effective ingredient consumption Leaching is poured, daily dispenser 1 time, continuous dispenser 7 days.
As further improvement of the invention, composition grain or the ratio of liquid and water are 1:10.
As further improvement of the invention, effective ingredient consumption is 80g/ mus.
The present invention has the advantages that:
1, Momordica grosvenori root extract extracting method is simple in the present invention, and of low cost, anti-root-knot nematode is with obvious effects;
2, the anti-root-knot nematode composition of the present invention is safe to use, degradable, to human body and environmentally friendly, will not cause to swash The residual of element, will not bring safety problem;
3, the anti-root-knot nematode composition of the present invention has good effect to prevention root-knot nematode;
4, moderate resistance root-knot nematode composition of the present invention is of low cost, and preparation method is simple, without putting into large-scale special equipment.
Description of the drawings
Fig. 1 is Momordica grosvenori root bionic extraction object preparation technology figure.
Specific implementation mode
Below in conjunction with the embodiment of the present invention, technical solution in the embodiment of the present invention carries out clear, complete description, Obviously, the embodiment described is the embodiment of the part of representative of the present invention, rather than whole embodiments, this field are general Other all embodiments that logical technical staff is obtained without making creative work belong to the protection of the present invention Range.
The preparation of 1 Siraitia grosvenorii bionic extraction object of embodiment
It follows the steps below:
(1) Momordica grosvenori root dried, crushed, sieved with 100 mesh sieve, obtain Momordica grosvenori root powder;
(2) preparation of bionic extraction liquid:0.2% sodium chloride, 0.32% pepsin and 0.02mol/L hydrochloric acid adjust pH =5.5;
(3) according to Momordica grosvenori root and bionic extraction liquid weight ratio 1:30 ratio mixing, 37 DEG C of heating water baths, plastic film Sealing, magneton extract 120min, and filtrate is collected in filtering, and water-bath is concentrated into 1/4 volume, collects concentrate;
(4) by the concentrate in step (3) according to volume ratio 1:4 mix with ethyl acetate acetic acid butyl acetate solution, extraction 3 It is secondary, organic phase is collected, vacuum rotary steam removes solvent, obtains white powder, as Momordica grosvenori root bionic extraction object.
The Chinese medicinal granule of 2 object of bionic extraction containing Momordica grosvenori root of embodiment
Raw material forms:15 parts of Momordica grosvenori root bionic extraction object, 15 parts of derris root, 10 parts of tripterygium wilfordii, 4 parts of quick lime;
Preparation process:
(1) derris root, tripterygium wilfordii are crushed into postemphasis amount than 1:10 water impregnates 1h, decocts 2h, filtering, then by filter residue Add weight is than 1:8 water decocts 2h, and it is 0.9-1.5 to be concentrated into density, and maltodextrin is added, and spray drying obtains spray powder;
(2) be added in spray powder prepared by step (1) Momordica grosvenori root bionic extraction object, quick lime and 90% ethyl alcohol it is molten Liquid makes pellet, dry, whole grain, and reducing blood lipid particle is made in packaging.
The traditional Chinese medicine liquid of 3 object of bionic extraction containing Momordica grosvenori root of embodiment
Raw material forms:13 parts of Momordica grosvenori root bionic extraction object, 12 parts of derris root, 7 parts of tripterygium wilfordii, 2 parts of quick lime.
Preparation process:Derris root, tripterygium wilfordii are crushed into postemphasis amount than 1:10 water impregnates 1h, decocts 2h, filters, then By filter residue add weight than 1:8 water decocts 2h, and it is 0.6-0.8 to be concentrated into density, and the Momordica grosvenori root being added after water dissolution is bionical to be carried Object and quick lime are taken, is uniformly mixed, continues to be concentrated into density to be 0.7-0.9, traditional Chinese medicine liquid is made;
The traditional Chinese medicine liquid of 4 object of bionic extraction containing Momordica grosvenori root of embodiment
Raw material forms:10 parts of Momordica grosvenori root bionic extraction object, 10 parts of derris root, 5 parts of tripterygium wilfordii, 2 parts of quick lime.
Preparation process:Derris root, tripterygium wilfordii are crushed into postemphasis amount than 1:10 water impregnates 1h, decocts 2h, filters, then By filter residue add weight than 1:8 water decocts 2h, and it is 0.6-0.8 to be concentrated into density, and the Momordica grosvenori root being added after water dissolution is bionical to be carried Object and quick lime are taken, is uniformly mixed, continues to be concentrated into density to be 0.7-0.9, traditional Chinese medicine liquid is made;
1 field experiment of test example
Field experiment carries out on the Siraitia grosvenorii planting site in bayhead bridge southern exposure village of Wugang City of Hubei Province respectively;
Experiment sets 5 groups altogether, if every group 2 mu, using daily management method, field management, soil are carried out by same person For sandy loam, easy infection root knot nematode disease.
Test group:1 group of Siraitia grosvenorii bionic extraction object water distribution prepared using embodiment 1 sprays field, and 2 groups are adopted The traditional Chinese medicine liquid water distribution of the object of bionic extraction containing Momordica grosvenori root prepared with embodiment 3 sprays field;3,4 groups of selection root knots Nematode common pesticides are sprayed, wherein 3 groups using nematode must a gram water distribution field is sprayed, 4 groups use happy lot polygamy water Field is sprayed;5 groups spray field using sophora flavescens ait alkali extract water distribution;In use, drug or concentrate Proportioning with water is 1:10, Siraitia grosvenorii root is sprayed with liquid, the amount of spraying by 50-100g/ mus of effective ingredient consumption into Row, 1 time a day, continuous 7 days, wherein mu plantation amount was identical, during plantation to the treatment of Meloidogyne Incognita of Luohanguo into Row statistics, the results are shown in Table 1:
Effect contrast table after the treatment of 1 each group Meloidogyne incognita of table
Group Cure rate % Total effective rate %
1 group 85% 90%
2 groups 90% 97%
3 groups 84% 89%
4 groups 69% 73%
5 groups 60% 71%
It can be seen from the above result that Siraitia grosvenorii bionic extraction object, the object of bionic extraction containing Siraitia grosvenorii traditional Chinese medicine liquid of the invention, nematode Must gram, happy lot it is mostly effective to the prevention of Meloidogyne Incognita of Luohanguo with kuh-seng alkali extract, wherein happy lot is mostly and matrine The effect of extract is poor, and total effective rate is only 71%-73%;Although the cure rate that nematode must be gram with Siraitia grosvenorii bionic extraction object It is close with total effective rate, but pesticide nematode must gram contain a large amount of chemical toxic ingredients, pollute environment, in contrast, Siraitia grosvenorii Not only effect is good and environmental-friendly for the traditional Chinese medicine liquid of bionic extraction object and the object of bionic extraction containing Siraitia grosvenorii of the present invention, to people Body nonhazardous acts on, and is the drug of best prevention root-knot nematode.Wherein, present invention bionic extraction containing Siraitia grosvenorii object traditional Chinese medicine liquid Effect is optimal, and total effective rate reaches 97%.
Those skilled in the art is not under conditions of departing from the spirit and scope of the present invention of claims determination, also Various modifications can be carried out to the above content.Therefore the scope of the present invention is not limited in above explanation, but by The range of claims determines.

Claims (10)

1. a kind of Chinese medicine composition of the object of bionic extraction containing Momordica grosvenori root, which is characterized in that composition by following parts by weight original Material is prepared:10-15 parts of Momordica grosvenori root bionic extraction object, 10-15 parts of derris root, 5-10 parts of tripterygium wilfordii, 2-5 parts of quick lime.
2. composition according to claim 1, which is characterized in that the composition by following parts by weight raw material prepare and At:13 parts of Momordica grosvenori root bionic extraction object, 12 parts of derris root, 7 parts of tripterygium wilfordii, 2 parts of quick lime.
3. composition according to claim 1, which is characterized in that the preparation method of the Momordica grosvenori root bionic extraction object, step It is rapid as follows:
(1) Momordica grosvenori root dried, crushed, sieved with 100 mesh sieve, obtain Momordica grosvenori root powder;
(2) according to Momordica grosvenori root and bionic extraction liquid weight ratio 1:30 ratio mixing, 37 DEG C of heating water baths, plastic film are close Envelope, magneton extract 120min, and filtrate is collected in filtering, and water-bath is concentrated into 1/4 volume, collects concentrate;
(3) by the concentrate in step (2) according to volume ratio 1:4 mix with ethyl acetate acetic acid butyl acetate solution, extract 3 times, receive Collect organic phase, vacuum rotary steam removes solvent, obtains white powder, as Momordica grosvenori root extract.
4. composition according to claim 3, which is characterized in that the bionic extraction liquid is by 0.2% sodium chloride, 0.32% stomach Protease and 0.02mol/L hydrochloric acid are formulated.
5. composition according to claim 4, which is characterized in that the bionic extraction liquid pH is 4~6.
6. composition according to claim 1 or claim 2, which is characterized in that the composition can according to known pharmaceutical technology (tcd) To be prepared into granule, concentrate.
7. a kind of Chinese medicine composition of object of bionic extraction containing Momordica grosvenori root according to claim 1 or 2 is preventing Siraitia grosvenorii Application in root-knot nematode.
8. a kind of method of prevention Meloidogyne incognita, which is characterized in that the Chinese medicine composition for preparing claims 1 or 2 Particle or liquid and water are according to weight ratio 1:1-1:20 are configured to liquid, to Siraitia grosvenorii by 50-100g/ mus of effective ingredient consumption It is sprayed or is poured, daily dispenser 1 time, continuous dispenser 7 days.
9. according to the method described in claim 8, it is characterized in that, the ratio of the composition grain or liquid and water is 1: 10。
10. according to the method described in claim 8, it is characterized in that, the effective ingredient consumption is 80g/ mus.
CN201810211464.5A 2018-03-15 2018-03-15 The application of the anti-root-knot nematode of Chinese medicine composition of the object of bionic extraction containing Momordica grosvenori root Pending CN108419809A (en)

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