CN108404197A - A kind of styptic powder - Google Patents
A kind of styptic powder Download PDFInfo
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- CN108404197A CN108404197A CN201810398195.8A CN201810398195A CN108404197A CN 108404197 A CN108404197 A CN 108404197A CN 201810398195 A CN201810398195 A CN 201810398195A CN 108404197 A CN108404197 A CN 108404197A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/08—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/0005—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/02—Surgical adhesives or cements; Adhesives for colostomy devices containing inorganic materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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Abstract
The invention discloses a kind of styptic powders, belong to field of medicaments.Styptic powder of the present invention includes 3 ~ 5 parts of chitosans, 4 ~ 7 parts of Bletilla glucomannans, 15 ~ 20 parts of oyster shell whitings, 3 ~ 8 parts of antimicrobial additives, 50 ~ 70 parts of styptic powder base-materials, 20 ~ 30 parts of styptic activity objects to get styptic powder.The present invention solves current styptic powder poor adhesion, and anthemorrhagic performance is poor, adds the substance of some quick-acting haemostatic powders, the problem of cannot comparatively fast being absorbed by body and be promoted regeneration.
Description
Technical field
The invention belongs to field of medicaments, and in particular to a kind of styptic powder.
Background technology
In daily life, how fast and effeciently to stop blooding is problem urgently to be resolved hurrily.Effectively stop in surgical operation
Blood means can be such that operation successfully carries out, and improve the success rate of operation, while can reduce the generation of postoperative complications；It is fighting
On field, effective hemostasis means are then one of the maximally efficient measures for reducing soldier's injures and deaths.Hemostatic material is in various surgery hands
In art, to reducing bleeding, shortens operating time and patient is postoperative important influence.In surgery patients and receive anti-freezing
In the patient of drug therapy, for example, bleeding meeting bodily tissue, organ or bone bring some problems, the prestige of life is ultimately caused
The side of body, uncontrollable bleeding can lead to shock and death.Clinically, it is often used fibrin originally to stop blooding, still, to protect
The biological hemostatic activity for holding these protein formulations, generally requires the special storages environment such as low temperature, this also significantly limits egg
The use of white preparation hemostat.In Clinical practice, the shape of hemostatic material is also applied to cause prodigious influence, hemostatic material
It is required to be attached to the surface of complex organ, this promotes the generations of styptic powder.
Styptic powder is powdered hemostatic agent, has the advantages that not limited by surface of a wound size and position, with surface of a wound contact area
Greatly, it can effectively stop blooding, promote regeneration and functional rehabilitation effect.Existing collagen class hemostasis pulvis, chitosan class are stopped at present
Blood meal agent, starch hemostasis pulvis, cellulose family hemostasis pulvis, although all achieving certain effect, existing styptic powder
Agent adhesiveness is poor, and anthemorrhagic performance is general, adds the substance of some quick-acting haemostatic powders wherein, such as fibrin ferment, tranexamic acid, there is one
Fixed immunogenicity cannot be absorbed by organisms or cannot promote regeneration, cannot meet the needs of people.Therefore, raw
A kind of better styptic powder of haemostatic effect of output is a necessary thing.
Invention content
The technical problems to be solved by the invention：For current styptic powder poor adhesion, anthemorrhagic performance is poor, and it is fast to add some
The substance of short stopping blood provides a kind of styptic powder the problem of cannot comparatively fast being absorbed by body and be promoted regeneration.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of styptic powder, meter includes following component, 3 ~ 5 parts of chitosans, 4 ~ 7 parts of Bletilla glucomannans, 15 ~ 20 parts of shells in parts by weight
Powder, 3 ~ 8 parts of antimicrobial additives, which is characterized in that further include 50 ~ 70 parts of styptic powder base-materials, 20 ~ 30 parts of styptic activity objects；
The preparation method of the styptic powder base-material is according to the mass fraction, to take 6 ~ 9 parts of pretreated straw matrix objects, 1 ~ 4 part of carbonic acid
Sodium, 2 ~ 4 parts of sodium bicarbonates, 80 ~ 100 parts of distilled water mixing, are stirred in 30 ~ 40 DEG C, obtain stirring mixture, take and be stirred
Object takes stirring mixture in mass ratio 10：1：1 is added 2,2,6,6- tetramethyl piperidine oxides, sodium bromide, is stirred in 45 ~ 50 DEG C
30 ~ 50min of mixing is mixed, mixture is obtained, takes mixture in mass ratio 10：3 are added liquor natrii hypochloritis, are stirred, and centrifuge, take
Supernatant A, adjust pH to 3 ~ 4, in mass ratio 1：3 are added absolute ethyl alcohol, stand, and take precipitation to get styptic powder base-material；
The preparation method of the styptic activity object, includes the following steps：
A. silk cocoon in mass ratio 2 is taken：1：50 are added sodium carbonate, distilled water, are stirred in 95 ~ 100 DEG C, obtain mixed liquor, take chlorine
Change calcium in mass ratio 1：10 are added ethyl alcohol, obtain mixed liquor A, take mixed liquor in mass ratio 1：8 are added mixed liquor A, are stirred in 80 ~ 90 DEG C
Mixing is mixed, filters, smoke filtrate is taken to dialyse, is freeze-dried, obtains dried object；
B. dried object in mass ratio 1 is taken：45 are added distilled water mixing, add the alkali protease of dried object quality 2 ~ 5%, enzyme
Solution, obtains primary zymolyte, takes compound fertilizer production in mass ratio 3：100 are added primary zymolyte, enzymolysis, and enzyme deactivation is filtered, taken
Smoke filtrate A dryings are to get styptic activity object.
The preparation method of the pretreated straw matrix object, includes the following steps：
（1）Take stalk in mass ratio 70 ~ 75：4~7：2 ~ 4 are added phosphorus pentoxide, sulfuric acid mixing, are stirred in 60 ~ 65 DEG C,
Filtering takes filter residue through distilling water washing, obtains washings, take washings in mass ratio 5：2 are added sodium hydroxide solution, in 50 ~ 70
DEG C stand, filtering, take filter residue A；
（2）According to the mass fraction, 1 ~ 3 part of peptone, 0.3 ~ 0.6 part of yeast extract, 0.8 ~ 1.5 part of sodium chloride, 100 parts of distilled water are taken
Mixing, sterilizing, obtains sterilizing object, takes serratia marcescens to be seeded in sterilizing object by 2% inoculum concentration and cultivates, super then at -4 ~ -2 DEG C
Sonication, centrifugation, takes supernatant, takes filter residue A in mass ratio 5：3 are added supernatant, in 40 ~ 50 DEG C of standings, add filter residue A matter
The hydrogen peroxide of amount 30 ~ 40% adjusts pH to 9 ~ 10, is stirred, and filters, and takes and takes out filter residue and drying to get pretreated straw matrix
Object.
The antimicrobial additive is that tobacco waste is taken to dry, pulverize sieving, collects sieving particle, according to the mass fraction,
8 ~ 12 portions of arbor-vitaes, 5 ~ 8 parts of cattail pollens, 4 ~ 7 parts of sieving particles, 80 ~ 90 parts of methanol are taken, is stirred in 50 ~ 60 DEG C, is filtered, take filter
Liquid is concentrated under reduced pressure, obtains concentrate, take concentrate in mass ratio 1：5：7 are added distilled water, ethyl acetate mixes, and adjusting pH to 2 ~
3, extraction takes extract liquor rotary evaporation, then at 200 ~ 250 DEG C of 2 ~ 3h of heat preservation, is cooled to room temperature, freeze-drying adds to get antibacterial
Add object.
Compared with other methods, advantageous effects are the present invention：
（1）The present invention is raw material using stalk, and phosphorus pentoxide, sulfuric acid is added, and the gas of formation carries out microexplosion to stalk and assists
It with sig water processing, is combined with the moisture inside rice straw and generates sulfuric acid, rice straw generates a large amount of heat, makes table in situ
Face fibre structure is burst, and with surface lignin esterification occurs for sulfuric acid again, and a part of lignin loses the guarantor to holocellulose
Shield acts on, and in later stage alkali process, lignin is easier to dissolve out so that the rupture of stalk densification lignocellulosic structure loosens, together
When introduce Serratia enzyme, hydrogen peroxide oxidation decoloration is carried out to it, passes through oxidative degradation hydroxyl, aminobenzene class
Close object, and the lignocellulosic in stalk as have benzene ring structure macromolecular compound, can also by ring-opening reaction
It is degraded, improves the purity and crystallinity of raw material；
（2）Stalk after treatment is raw material by the present invention, by introducing 2,2,6,6- tetramethyl piperidine oxides, sodium bromide
And sodium hypochlorite, pretreated stalk is aoxidized, system aoxidizes to form C6 primary hydroxyls, to 2,2,6,6- tetramethyl piperidines
Oxide ion carries out nucleophillic attack, forms very unstable intermediate state TEMPOH and aldehyde, and acid undergoes what contracting was waken up in aqueous solution
Intermediate state is oxidized to form acid, and azanol TEMPOH loses proton and obtains, and oxidation continues, and forms cyclic oxidation system, and oxidation is fine
Acidic carboxypolymer contained by dimension element can form brown gummy blob of viscose, to block blood capillary in conjunction with the iron ion in hemoglobin
Pipe end bleeding, while the absorption and aggregation of blood platelet can be accelerated, to accelerate blood coagulation, what main active substances were aoxidized
Cellulose has swellability, can be oppressed in blood capillary after rapid swelling, to play anastalsis, tool
There is preferable swelling disintegrating property, chitosan, Bletilla glucomannan is coordinated to improve the adhesive bond performance of the coagulant material and the surface of a wound；
（3）The present invention utilizes and first passes through calcium chloride, ethyl alcohol, water-soluble salt solution solution silk cocoon, then passes through alkali protease and composite flavor
Enzyme complex enzyme hydrolysis, the site of effect peptide bond is more when digesting fibroin albumen, the peptide bond of main function hydrophobicity hydroxyl, and to crystallization
The peptide bond formed between amino acid in area has specificity, and lysine and arginine in fibroin albumen can be made to ionize out H+Make
Styptic activity substance is integrally negatively charged, is added in blood, by entering the porosity and looseness pipeline of styptic powder base-material, absorbs blood
In moisture, can accelerating fibers proteinogen absorb blood formation, with red blood cell bond after form grid, increase blood flow
Resistance causes blood viscosity to increase, and is conducive to blood clotting, while small-molecular peptides have the characteristic for easily penetrating into the surface of a wound, be easier to by
Matrix absorption, the activity of active cell promote regeneration healing；
（4）Tobacco waste, arbor-vitae, cattail pollen is added in the present invention, and phenolic acid compound and flavones main active have anti-
Bacterium bacteriostatic activity prevents the infection of the tissue surface of a wound, improves anthemorrhagic performance.
Specific implementation mode
Tobacco waste：It is derived from tobacco processing factory of Nanxiong City of Guangdong Province, with the low secondary cigarette that do not purchased by tobacco purchase station
Leaf is as tobacco waste material.
Bletilla glucomannan：It is purchased from Guangzhou Fei Ke Fine Chemical Co., Ltd.
Antimicrobial additive：It takes tobacco waste to dry, pulverize 40 mesh sieve, collects sieving particle and take according to the mass fraction
8 ~ 12 portions of arbor-vitaes, 5 ~ 8 parts of cattail pollens, 4 ~ 7 parts of sieving particles, 80 ~ 90 parts of methanol, are stirred 1 ~ 2h in 50 ~ 60 DEG C, filter, take
Filtrate is concentrated under reduced pressure, obtains concentrate, take concentrate in mass ratio 1：5：7 are added distilled water, ethyl acetate mixing, adjust pH to 2
~ 3,2 ~ 3h is extracted, extract liquor rotary evaporation is taken, then at 200 ~ 250 DEG C of 2 ~ 3h of heat preservation, is cooled to room temperature, freeze-drying is to get anti-
Bacterium additive.
Styptic powder base-material：
（1）Take stalk in mass ratio 70 ~ 75：4~7：2 ~ 4 are added phosphorus pentoxide, the sulfuric acid that mass fraction is 80% mixes, in 60
~ 65 DEG C are stirred 1 ~ 2h, and filtering takes filter residue through distilling water washing, obtains washings, take washings in mass ratio 5：2 additions are dense
Degree is the sodium hydroxide solution of 0.1mol/L, and 2 ~ 3h is stood in 50 ~ 70 DEG C, and filtering takes filter residue A；
（2）According to the mass fraction, 1 ~ 3 part of peptone, 0.3 ~ 0.6 part of yeast extract, 0.8 ~ 1.5 part of sodium chloride, 100 parts of distilled water are taken
Mixing, 121 DEG C of sterilizing 20min, obtains sterilizing object, takes serratia marcescens to be seeded in sterilizing object by 2% inoculum concentration, in 35 ~ 40
DEG C culture 24 ~ 36h, then at -4 ~ -2 DEG C supersound process 20 ~ 30min, centrifugation, take supernatant, take filter residue A in mass ratio 5：3 are added
Supernatant stands 1 ~ 2h in 40 ~ 50 DEG C, adds the hydrogen peroxide that the mass fraction of filter residue A mass 30 ~ 40% is 15%, adjusts pH
To 9 ~ 10, it is stirred 2 ~ 3h, is filtered, is taken pumping filter residue and drying, obtain pretreated straw matrix object；
（3）According to the mass fraction, 6 ~ 9 parts of pretreated straw matrix objects, 1 ~ 4 part of sodium carbonate, 2 ~ 4 parts of sodium bicarbonates, 80 ~ 100 are taken
Part distilled water mixing, is stirred 40 ~ 50min in 30 ~ 40 DEG C, obtains stirring mixture, take stirring mixture, take stirring mixture
In mass ratio 10：1：1 is added 2,2,6,6- tetramethyl piperidine oxides, sodium bromide, and 30 ~ 50min is stirred in 45 ~ 50 DEG C,
Mixture is obtained, mixture in mass ratio 10 is taken：3 are added the liquor natrii hypochloritis that mass fraction is 21%, are stirred 1 ~ 2h, from
The heart, takes supernatant A, adjusts pH to 3 ~ 4, and in mass ratio 1：3 addition absolute ethyl alcohols, standing 18 ~ for 24 hours, take precipitation to get styptic powder
Base-material.
Styptic activity object：A. silk cocoon in mass ratio 2 is taken：1：50 are added sodium carbonate, distilled water, are stirred in 95 ~ 100 DEG C
1 ~ 2h obtains mixed liquor, takes calcium chloride in mass ratio 1：10 are added the ethyl alcohol that mass fraction is 85%, obtain mixed liquor A, take mixed liquor
In mass ratio 1：8 are added mixed liquor A, and 2 ~ 3h is stirred in 80 ~ 90 DEG C, filter, smoke filtrate is taken to dialyse, and are freeze-dried, obtain dry
Dry object；
B. dried object in mass ratio 1 is taken：45 are added distilled water mixing, add the alkali protease of dried object quality 2 ~ 5%, in
PH8.5,55 ~ 60 DEG C of 2 ~ 3h of enzymolysis, obtain primary zymolyte, take compound fertilizer production in mass ratio 3：100 are added primary enzymolysis
Object, in pH6.5,45 ~ 50 DEG C of 1 ~ 2h of enzymolysis, enzyme deactivation filters, and takes smoke filtrate A dryings to get styptic activity object.
A kind of styptic powder, according to the mass fraction, including 3 ~ 5 parts of chitosans, 4 ~ 7 parts of Bletilla glucomannans, 15 ~ 20 parts of oyster shell whitings, 3 ~ 8
Part antimicrobial additive, 50 ~ 70 parts of styptic powder base-materials, 20 ~ 30 parts of styptic activity objects are to get styptic powder.
Styptic powder application method：Styptic powder is externally applied to hemorrhagic trauma surface, is stood, until blood coagulation.
Tobacco waste：It is derived from tobacco processing factory of Nanxiong City of Guangdong Province, with the low secondary cigarette that do not purchased by tobacco purchase station
Leaf is as tobacco waste material.
Bletilla glucomannan：It is purchased from Guangzhou Fei Ke Fine Chemical Co., Ltd.
Antimicrobial additive：It takes tobacco waste to dry, pulverize 40 mesh sieve, collects sieving particle and take according to the mass fraction
8 portions of arbor-vitaes, 5 parts of cattail pollens, 4 parts of sieving particles, 80 parts of methanol, are stirred 1h in 50 DEG C, filter, take filtrate, be concentrated under reduced pressure, obtain
Concentrate takes concentrate in mass ratio 1：5：7 are added distilled water, ethyl acetate mixing, adjust pH to 2, extract 2h, take extract liquor
Rotary evaporation is cooled to room temperature, is freeze-dried to get antimicrobial additive then at 200 DEG C of heat preservation 2h.
Styptic powder base-material：（1）Take stalk in mass ratio 70：4：2 are added phosphorus pentoxide, mass fraction as 80% sulfuric acid
Mixing, 1h is stirred in 60 DEG C, is filtered, and is taken filter residue through distilling water washing, is obtained washings, take washings in mass ratio 5：2 add
The sodium hydroxide solution for entering a concentration of 0.1mol/L, 2h is stood in 50 DEG C, and filtering takes filter residue A；
（2）According to the mass fraction, 1 part of peptone, 0.3 part of yeast extract, 0.8 part of sodium chloride, 100 parts of distilled water is taken to mix, 121 DEG C
Sterilize 20min, obtains sterilizing object, and serratia marcescens is taken to be seeded in sterilizing object by 2% inoculum concentration, for 24 hours in 35 DEG C of cultures, then
20min is ultrasonically treated in -4 DEG C, centrifugation takes supernatant, takes filter residue A in mass ratio 5：3 are added supernatant, and 1h is stood in 40 DEG C,
The hydrogen peroxide that the mass fraction of filter residue A mass 30% is 15% is added, pH to 9 is adjusted, is stirred 2h, filters, takes suction filtration slag
It is dry, obtain pretreated straw matrix object；
（3）According to the mass fraction, take 6 parts of pretreated straw matrix objects, 1 part of sodium carbonate, 2 parts of sodium bicarbonates, 80 parts of distilled water mixed
It closes, is stirred 40min in 30 DEG C, obtains stirring mixture, take stirring mixture, take stirring mixture in mass ratio 10：1：1 adds
Enter 2,2,6,6- tetramethyl piperidine oxides, sodium bromide, is stirred 30min in 45 DEG C, obtains mixture, take mixture by quality
Than 10：3 are added the liquor natrii hypochloritis that mass fraction is 21%, are stirred 1h, centrifuge, and take supernatant A, adjust pH to 3, press
Mass ratio 1：3 are added absolute ethyl alcohol, stand 18h, take precipitation to get styptic powder base-material.
Styptic activity object：A. silk cocoon in mass ratio 2 is taken：1：50 are added sodium carbonate, distilled water, and 1h is stirred in 95 DEG C,
Mixed liquor is obtained, calcium chloride in mass ratio 1 is taken：10 are added the ethyl alcohol that mass fraction is 85%, obtain mixed liquor A, take mixed liquor by quality
Than 1：8 are added mixed liquor A, and 2h is stirred in 80 DEG C, filter, smoke filtrate is taken to dialyse, and are freeze-dried, obtain dried object；
B. dried object in mass ratio 1 is taken：45 are added distilled water mixing, add the alkali protease of dried object quality 2%, in
PH8.5,55 DEG C of enzymolysis 2h, obtain primary zymolyte, take compound fertilizer production in mass ratio 3：100 are added primary zymolyte, in
PH6.5,45 DEG C of enzymolysis 1h, enzyme deactivation filter, and take smoke filtrate A dryings to get styptic activity object.
A kind of styptic powder, according to the mass fraction, including 3 parts of chitosans, 4 parts of Bletilla glucomannans, 15 parts of oyster shell whitings, 3 parts of antibacterials add
Add object, 50 parts of styptic powder base-materials, 20 parts of styptic activity objects to get styptic powder.
Styptic powder application method：Styptic powder is externally applied to hemorrhagic trauma surface, is stood, until blood coagulation.
Tobacco waste：It is derived from tobacco processing factory of Nanxiong City of Guangdong Province, with the low secondary cigarette that do not purchased by tobacco purchase station
Leaf is as tobacco waste material.
Bletilla glucomannan：It is purchased from Guangzhou Fei Ke Fine Chemical Co., Ltd.
Antimicrobial additive：It takes tobacco waste to dry, pulverize 40 mesh sieve, collects sieving particle and take according to the mass fraction
12 portions of arbor-vitaes, 8 parts of cattail pollens, 7 parts of sieving particles, 90 parts of methanol, are stirred 2h in 60 DEG C, filter, take filtrate, be concentrated under reduced pressure,
Concentrate is obtained, concentrate in mass ratio 1 is taken：5：7 are added distilled water, ethyl acetate mixing, adjust pH to 3, extract 3h, take extraction
Liquid rotary evaporation is cooled to room temperature, is freeze-dried to get antimicrobial additive then at 250 DEG C of heat preservation 3h.
Styptic powder base-material：（1）Take stalk in mass ratio 75：7：4 are added phosphorus pentoxide, mass fraction as 80% sulfuric acid
Mixing, 2h is stirred in 65 DEG C, is filtered, and is taken filter residue through distilling water washing, is obtained washings, take washings in mass ratio 5：2 add
The sodium hydroxide solution for entering a concentration of 0.1mol/L, 3h is stood in 70 DEG C, and filtering takes filter residue A；
（2）According to the mass fraction, 3 parts of peptones, 0.6 part of yeast extract, 1.5 parts of sodium chloride, 100 parts of distilled water is taken to mix, 121 DEG C
Sterilize 20min, obtains sterilizing object, and serratia marcescens is taken to be seeded in sterilizing object by 2% inoculum concentration, and 36h is cultivated in 40 DEG C, then
30min is ultrasonically treated in -2 DEG C, centrifugation takes supernatant, takes filter residue A in mass ratio 5：3 are added supernatant, and 2h is stood in 50 DEG C,
The hydrogen peroxide that the mass fraction of filter residue A mass 40% is 15% is added, pH to 10 is adjusted, is stirred 3h, filters, takes suction filtration slag
It is dry, obtain pretreated straw matrix object；
（3）According to the mass fraction, take 9 parts of pretreated straw matrix objects, 4 parts of sodium carbonate, 4 parts of sodium bicarbonates, 100 parts of distilled water mixed
It closes, is stirred 50min in 40 DEG C, obtains stirring mixture, take stirring mixture, take stirring mixture in mass ratio 10：1：1 adds
Enter 2,2,6,6- tetramethyl piperidine oxides, sodium bromide, is stirred 50min in 50 DEG C, obtains mixture, take mixture by quality
Than 10：3 are added the liquor natrii hypochloritis that mass fraction is 21%, are stirred 2h, centrifuge, and take supernatant A, adjust pH to 4, press
Mass ratio 1：3 are added absolute ethyl alcohol, stand for 24 hours, take precipitation to get styptic powder base-material.
Styptic activity object：A. silk cocoon in mass ratio 2 is taken：1：50 are added sodium carbonate, distilled water, and 2h is stirred in 100 DEG C,
Mixed liquor is obtained, calcium chloride in mass ratio 1 is taken：10 are added the ethyl alcohol that mass fraction is 85%, obtain mixed liquor A, take mixed liquor by quality
Than 1：8 are added mixed liquor A, and 3h is stirred in 90 DEG C, filter, smoke filtrate is taken to dialyse, and are freeze-dried, obtain dried object；
B. dried object in mass ratio 1 is taken：45 are added distilled water mixing, add the alkali protease of dried object quality 5%, in
PH8.5,60 DEG C of enzymolysis 3h, obtain primary zymolyte, take compound fertilizer production in mass ratio 3：100 are added primary zymolyte, in
PH6.5,50 DEG C of enzymolysis 2h, enzyme deactivation filter, and take smoke filtrate A dryings to get styptic activity object.
A kind of styptic powder, according to the mass fraction, including 5 parts of chitosans, 7 parts of Bletilla glucomannans, 20 parts of oyster shell whitings, 8 parts of antibacterials add
Add object, 70 parts of styptic powder base-materials, 30 parts of styptic activity objects to get styptic powder.
Styptic powder application method：Styptic powder is externally applied to hemorrhagic trauma surface, is stood, until blood coagulation.
Tobacco waste：It is derived from tobacco processing factory of Nanxiong City of Guangdong Province, with the low secondary cigarette that do not purchased by tobacco purchase station
Leaf is as tobacco waste material.
Bletilla glucomannan：It is purchased from Guangzhou Fei Ke Fine Chemical Co., Ltd.
Antimicrobial additive：It takes tobacco waste to dry, pulverize 40 mesh sieve, collects sieving particle and take according to the mass fraction
10 portions of arbor-vitaes, 7 parts of cattail pollens, 6 parts of sieving particles, 85 parts of methanol, are stirred 1h in 55 DEG C, filter, take filtrate, be concentrated under reduced pressure,
Concentrate is obtained, concentrate in mass ratio 1 is taken：5：7 are added distilled water, ethyl acetate mixing, adjust pH to 2, extract 2h, take extraction
Liquid rotary evaporation is cooled to room temperature, is freeze-dried to get antimicrobial additive then at 225 DEG C of heat preservation 2h.
Styptic powder base-material：（1）Take stalk in mass ratio 73：5：3 are added phosphorus pentoxide, mass fraction as 80% sulfuric acid
Mixing, 1h is stirred in 63 DEG C, is filtered, and is taken filter residue through distilling water washing, is obtained washings, take washings in mass ratio 5：2 add
The sodium hydroxide solution for entering a concentration of 0.1mol/L, 2h is stood in 60 DEG C, and filtering takes filter residue A；
（2）According to the mass fraction, 2 parts of peptones, 0.4 part of yeast extract, 1.2 parts of sodium chloride, 100 parts of distilled water is taken to mix, 121 DEG C
Sterilize 20min, obtains sterilizing object, and serratia marcescens is taken to be seeded in sterilizing object by 2% inoculum concentration, and 30h is cultivated in 37 DEG C, then
25min is ultrasonically treated in -3 DEG C, centrifugation takes supernatant, takes filter residue A in mass ratio 5：3 are added supernatant, and 1h is stood in 45 DEG C,
The hydrogen peroxide that the mass fraction of filter residue A mass 35% is 15% is added, pH to 9 is adjusted, is stirred 2h, filters, takes suction filtration slag
It is dry, obtain pretreated straw matrix object；
（3）According to the mass fraction, take 8 parts of pretreated straw matrix objects, 3 parts of sodium carbonate, 3 parts of sodium bicarbonates, 90 parts of distilled water mixed
It closes, is stirred 45min in 35 DEG C, obtains stirring mixture, take stirring mixture, take stirring mixture in mass ratio 10：1：1 adds
Enter 2,2,6,6- tetramethyl piperidine oxides, sodium bromide, is stirred 40min in 47 DEG C, obtains mixture, take mixture by quality
Than 10：3 are added the liquor natrii hypochloritis that mass fraction is 21%, are stirred 1h, centrifuge, and take supernatant A, adjust pH to 3, press
Mass ratio 1：3 are added absolute ethyl alcohol, stand 21h, take precipitation to get styptic powder base-material.
Styptic activity object：A. silk cocoon in mass ratio 2 is taken：1：50 are added sodium carbonate, distilled water, and 1h is stirred in 97 DEG C,
Mixed liquor is obtained, calcium chloride in mass ratio 1 is taken：10 are added the ethyl alcohol that mass fraction is 85%, obtain mixed liquor A, take mixed liquor by quality
Than 1：8 are added mixed liquor A, and 2h is stirred in 85 DEG C, filter, smoke filtrate is taken to dialyse, and are freeze-dried, obtain dried object；
B. dried object in mass ratio 1 is taken：45 are added distilled water mixing, add the alkali protease of dried object quality 4%, in
PH8.5,57 DEG C of enzymolysis 2h, obtain primary zymolyte, take compound fertilizer production in mass ratio 3：100 are added primary zymolyte, in
PH6.5,47 DEG C of enzymolysis 1h, enzyme deactivation filter, and take smoke filtrate A dryings to get styptic activity object.
A kind of styptic powder, according to the mass fraction, including 4 parts of chitosans, 6 parts of Bletilla glucomannans, 17 parts of oyster shell whitings, 6 parts of antibacterials add
Add object, 60 parts of styptic powder base-materials, 25 parts of styptic activity objects to get styptic powder.
Styptic powder application method：Styptic powder is externally applied to hemorrhagic trauma surface, is stood, until blood coagulation.
Comparative example 1：It is essentially identical with the preparation method of embodiment 2, it has only the difference is that lacking styptic activity object.
Comparative example 2：It is essentially identical with the preparation method of embodiment 2, it has only the difference is that lacking styptic powder base-material.
Comparative example 3：The styptic powder of company of Nanchang City production.
Rabbit Femoral Artery Hemorrhage Model is respectively adopted in the styptic powder of above-described embodiment and comparative example to test, every group 10
Animal.Test result is listed in table 1.
Table 1：
| Test event | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Bleeding stopping period（min） | 1.02 | 1.0 | 1.01 | 0.96 | 1.08 | 2~2.02 |
| Wound healing time（It） | 3 | 3 | 3 | 4 | 3 | 6~7 |
In summary, styptic powder haemostatic effect of the invention is good, and wound healing time is short, fully meets use demand, is worth big
It pushes wide.
Claims (3)
1. a kind of styptic powder, meter includes following component, 3 ~ 5 parts of chitosans, 4 ~ 7 parts of Bletilla glucomannans, 15 ~ 20 parts of shells in parts by weight
Powder, 3 ~ 8 parts of antimicrobial additives, which is characterized in that further include 50 ~ 70 parts of styptic powder base-materials, 20 ~ 30 parts of styptic activity objects；
The preparation method of the styptic powder base-material is according to the mass fraction, to take 6 ~ 9 parts of pretreated straw matrix objects, 1 ~ 4 part of carbonic acid
Sodium, 2 ~ 4 parts of sodium bicarbonates, 80 ~ 100 parts of distilled water mixing, are stirred in 30 ~ 40 DEG C, obtain stirring mixture, take and be stirred
Object takes stirring mixture in mass ratio 10：1：1 is added 2,2,6,6- tetramethyl piperidine oxides, sodium bromide, is stirred in 45 ~ 50 DEG C
30 ~ 50min of mixing is mixed, mixture is obtained, takes mixture in mass ratio 10：3 are added liquor natrii hypochloritis, are stirred, and centrifuge, take
Supernatant A, adjust pH to 3 ~ 4, in mass ratio 1：3 are added absolute ethyl alcohol, stand, and take precipitation to get styptic powder base-material；
The preparation method of the styptic activity object, includes the following steps：
A. silk cocoon in mass ratio 2 is taken：1：50 are added sodium carbonate, distilled water, are stirred in 95 ~ 100 DEG C, obtain mixed liquor, take chlorine
Change calcium in mass ratio 1：10 are added ethyl alcohol, obtain mixed liquor A, take mixed liquor in mass ratio 1：8 are added mixed liquor A, are stirred in 80 ~ 90 DEG C
Mixing is mixed, filters, smoke filtrate is taken to dialyse, is freeze-dried, obtains dried object；
Take dried object in mass ratio 1：45 are added distilled water mixing, add the alkali protease of dried object quality 2 ~ 5%, digest,
Primary zymolyte is obtained, compound fertilizer production in mass ratio 3 is taken：100 are added primary zymolyte, enzymolysis, and enzyme deactivation filters, takes pumping
Filtrate A dryings are to get styptic activity object.
2. styptic powder according to claim 1, which is characterized in that the preparation method of the pretreated straw matrix object, packet
Include following steps：
（1）Take stalk in mass ratio 70 ~ 75：4~7：2 ~ 4 are added phosphorus pentoxide, sulfuric acid mixing, are stirred in 60 ~ 65 DEG C,
Filtering takes filter residue through distilling water washing, obtains washings, take washings in mass ratio 5：2 are added sodium hydroxide solution, in 50 ~ 70
DEG C stand, filtering, take filter residue A；
（2）According to the mass fraction, 1 ~ 3 part of peptone, 0.3 ~ 0.6 part of yeast extract, 0.8 ~ 1.5 part of sodium chloride, 100 parts of distilled water are taken
Mixing, sterilizing, obtains sterilizing object, takes serratia marcescens to be seeded in sterilizing object by 2% inoculum concentration and cultivates, super then at -4 ~ -2 DEG C
Sonication, centrifugation, takes supernatant, takes filter residue A in mass ratio 5：3 are added supernatant, in 40 ~ 50 DEG C of standings, add filter residue A matter
The hydrogen peroxide of amount 30 ~ 40% adjusts pH to 9 ~ 10, is stirred, and filters, and takes and takes out filter residue and drying to get pretreated straw matrix
Object.
3. styptic powder according to claim 1, which is characterized in that the antimicrobial additive is that tobacco waste is taken to dry,
It pulverizes and sieves, collects sieving particle, according to the mass fraction, take 8 ~ 12 portions of arbor-vitaes, 5 ~ 8 parts of cattail pollens, 4 ~ 7 parts of sieving particles, 80 ~ 90
Part methanol, is stirred in 50 ~ 60 DEG C, filters, takes filtrate, is concentrated under reduced pressure, obtains concentrate, take concentrate in mass ratio 1：5：7
Be added distilled water, ethyl acetate mixing, adjust pH to 2 ~ 3, extraction, take extract liquor rotary evaporation, then at 200 ~ 250 DEG C heat preservation 2 ~
3h is cooled to room temperature, and is freeze-dried to get antimicrobial additive.
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