CN108393100B - Micro-droplet generation and collection reaction system - Google Patents
Micro-droplet generation and collection reaction system Download PDFInfo
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- CN108393100B CN108393100B CN201710068834.XA CN201710068834A CN108393100B CN 108393100 B CN108393100 B CN 108393100B CN 201710068834 A CN201710068834 A CN 201710068834A CN 108393100 B CN108393100 B CN 108393100B
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention relates to the technical field of biochips, and discloses a micro-droplet generation and collection reaction system, which comprises: the device comprises a micro-droplet generator, a micro-droplet collection reactor and a connecting pipeline, wherein the connecting pipeline is detachably connected to a micro-droplet output interface of the micro-droplet generator and a micro-droplet input interface of the micro-droplet collection reactor and is used for communicating a micro-droplet outlet with a micro-droplet inlet. Compared with the prior art, the embodiment of the invention can not only separate the micro-droplet generator and the micro-droplet collecting reactor and respectively select materials and manufacture the micro-droplet generator and the micro-droplet collecting reactor, but also directly collect and react micro-droplets generated by the micro-droplet generator.
Description
Technical Field
The invention relates to the technical field of biochips, in particular to a micro-droplet generation and collection reaction system.
Background
In the art, droplets, also known as emulsions, comprise two mutually incompatible liquids, such as water and oil, and the droplets are usually present in the form of water-in-oil or oil-in-water at steady state.
The traditional liquid drop generation mainly adopts modes of oscillation, stirring, spraying and the like, the generated liquid drop has large particle size distribution and poor repeatability, and the application of the liquid drop in large-scale biochemical analysis with high flux and high sensitivity is greatly limited.
With the development of microfluidic technology, droplet technology has come to new revolution. The micro-droplet technology is a technology for dividing continuous fluid into discrete nano-liter or pico-liter droplets by utilizing the interaction between the flow shearing force and the surface tension between two immiscible liquids in a micro-scale channel prepared based on the micro-fluidic chip technology. Compared with the traditional liquid drop, the micro liquid drop prepared by the micro-fluidic chip has extremely low reagent consumption, thereby greatly reducing the consumption of expensive biochemical reagents; the monodispersity of the liquid drops is good, and the controllability and the repeatability of experimental results are good; each liquid drop is an independent closed microreactor, so that cross contamination is reduced; the specific surface area is large, so that the heat transfer speed and the chemical and biological reaction speed are accelerated; the integration and automation are facilitated, and the operation and the control are convenient; therefore, the method is an ideal choice in the fields of drug synthesis, screening, single cell analysis, accurate quantitative analysis of molecular biological protein and nucleic acid, material synthesis and the like.
In the prior art, the following two micro-droplet generation and collection reaction modes exist:
in the first mode, according to the principle, a micro-droplet generation unit and a micro-droplet collection reaction unit are integrated in a micro-fluidic chip and are respectively used for generating micro-droplets and storing the generated micro-droplets. In short, in this method, the droplet generation unit and the droplet collection reaction unit are integrated and not detachable, so that both the droplet generation unit and the droplet collection reaction unit are made of the same material.
The materials prepared by the method mainly comprise inorganic materials and some polymer materials.
Inorganic materials such as silicon, glass, and quartz glass have high processing costs and poor practicability, while polymer materials are easy to process and have low material costs, and are increasingly used in recent years.
At present, the polymer materials applied to the microfluidic chip mainly include PDMS (polydimethylsiloxane), PMMA (polymethyl methacrylate), PS (polystyrene), PC (polycarbonate), COC/COP (cyclic olefin copolymer), and the like, especially PDMS and PMMA, and have the characteristics of low cost, simple use, good optical properties, good insulating property, good chemical inertness, and the like, so that the polymer material is widely applied to the fields of microfluidic control and the like.
However, the polymer material has good air permeability and high thermal expansion performance, so that phenomena such as volatilization of water and oil phases, fusion and fracture of micro-droplets and the like of a micro-droplet biological reaction system are easily caused.
Therefore, in this method, it is difficult to select the most suitable material for the reaction, especially in a high-temperature reaction environment or a reaction environment requiring frequent hot and cold alternation.
The second mode adopts micro-droplet generation and micro-droplet collection reaction system separation. Briefly, micro-droplets prepared by the micro-fluidic chip are collected in a centrifuge tube, and then subsequent reaction and signal acquisition are performed.
Although the mode can select the most appropriate manufacturing materials for the micro-droplet generation unit and the micro-droplet collection reaction unit, the reaction of the micro-droplets in a centrifugal tube and the transfer of the micro-droplets in modules for preparation, collection reaction, signal detection and the like can cause the fusion of a large number of micro-droplets, thereby not only destroying the micro-droplets but also leading to complicated operation.
Disclosure of Invention
An object of an embodiment of the present invention is to provide a micro-droplet generating and collecting reaction system, which can separate a micro-droplet generator and a micro-droplet collecting reactor, respectively select and manufacture materials for the micro-droplet generator and the micro-droplet collecting reactor, and can directly perform a collecting reaction on micro-droplets generated by the micro-droplet generator.
In order to solve the above technical problems, an embodiment of the present invention provides a micro-droplet generation and collection reaction system, including: the device comprises a micro-droplet generator, a micro-droplet collecting reactor and a connecting pipeline;
the micro-droplet generator comprises a first liquid inlet channel, a second liquid inlet channel, a micro-droplet output channel, a first liquid inlet, a second liquid inlet, a micro-droplet outlet, a first liquid inlet interface, a second liquid inlet interface and a micro-droplet output interface, wherein the first liquid inlet channel, the second liquid inlet channel and the micro-droplet output channel are intersected at one point;
the micro-droplet collection reactor comprises a containing cavity for collecting micro-droplets, a micro-droplet inlet communicated with the containing cavity, and a micro-droplet input interface arranged at the micro-droplet inlet;
the connecting pipeline is detachably connected to the micro-droplet output interface and the micro-droplet input interface and is used for communicating the micro-droplet outlet with the micro-droplet inlet.
During the use, send into first inlet with first phase liquid through first inlet liquid interface, send into the second inlet with second phase liquid through second inlet liquid interface, through the first inlet liquid passageway, second inlet liquid passageway and the little liquid drop output channel that intersects in a bit, make the flow shearing force and the surface tension interact between these two mutually insoluble two-phase liquid, get into little liquid drop output channel with the little liquid drop of output to in proper order through little liquid drop output interface, connecting tube, little liquid drop input interface, and finally get into and hold the chamber. Moreover, the connecting pipeline is detachably connected to the micro-droplet output interface and the micro-droplet input interface, so that compared with the prior art, the micro-droplet generator and the micro-droplet collecting reactor can be separated and respectively manufactured by selecting materials, and micro-droplets generated by the micro-droplet generator can be directly collected and reacted.
Furthermore, the micro-droplet collection reactor further comprises an exhaust port communicated with the accommodating cavity and an exhaust interface arranged at the exhaust port, when micro-droplets enter the accommodating cavity, the exhaust interface can be opened to discharge residual air in the accommodating cavity to the outside so as to satisfy the condition that the micro-droplets are full of the whole accommodating cavity and avoid the generation of bubbles.
Preferably, the micro-droplet generator comprises a generator cover plate and a generator substrate, wherein the generator cover plate is provided with a first liquid inlet concave channel, a second liquid inlet concave channel and a micro-droplet output concave channel which are intersected at one point;
the first liquid inlet, the second liquid inlet and the micro-droplet outlet are respectively arranged on the generator cover plate in a penetrating manner, the first liquid inlet is communicated with the first liquid inlet concave channel, the second liquid inlet is communicated with the second liquid inlet concave channel, and the micro-droplet outlet is communicated with the micro-droplet output concave channel;
the generator cover plate is connected with the generator substrate, the first liquid inlet channel and the generator substrate form the first liquid inlet channel, the second liquid inlet channel and the generator substrate form the second liquid inlet channel, and the micro-droplet output channel and the generator substrate form the micro-droplet output channel;
the first liquid inlet interface, the second liquid inlet interface and the micro-droplet output interface are respectively bonded on the generator cover plate, and the manufacturing of the micro-droplet generator can be simplified through the splicing combination structure.
Further, the generator cover plate and/or the generator base plate are made of an inorganic material or a polymer material.
Preferably, the micro-droplet collection reactor comprises a collection reactor cover plate and a collection reactor base plate, and the collection reactor cover plate is provided with an accommodating cavity groove;
the micro-droplet inlet is penetratingly arranged on the collecting reactor cover plate and is communicated with the accommodating cavity groove;
the collecting reactor cover plate and the collecting reactor base plate are connected with each other, and the accommodating cavity groove and the collecting reactor base plate form the accommodating cavity;
the micro-droplet input interface is adhered to the collecting reactor cover plate, and the manufacturing of the micro-droplet collecting reactor can be simplified through the splicing combination structure.
Further, the collecting reactor cover plate and/or the collecting reactor base plate are made of an inorganic material and/or a hard polymer material, such as PC, PS, PMMA, etc.
In another preferred embodiment, the micro droplet collection reactor comprises a collection reactor top plate, a collection reactor partition plate and a collection reactor bottom plate;
the micro-droplet inlet is penetratingly arranged on the top plate of the collection reactor;
the collecting reactor partition plate is provided with an accommodating cavity through hole;
the collecting reactor top plate, the collecting reactor partition plate and the collecting reactor bottom plate are sequentially connected, and the collecting reactor top plate, the accommodating cavity through hole and the collecting reactor bottom plate form the accommodating cavity;
the micro-droplet input interface is adhered to the top plate of the collection reactor, and the manufacturing of the micro-droplet collection reactor can be simplified through the splicing combination structure.
Further, the collecting reactor top plate and/or the collecting reactor bottom plate are made of an inorganic material and/or a hard polymer material, such as PC, PS, PMMA, etc.
In addition, the collecting reactor partition is made of a colloidal material so that the collecting reactor partition can be directly bonded to the collecting reactor top plate and the collecting reactor bottom plate.
In addition, the height of the accommodating cavity is H, and the diameter of the micro-droplet output by the micro-droplet generator is H, so that 0.5H is more than H and less than 2H, therefore, the micro-droplet can only exist in the accommodating cavity in a layer form, and the subsequent experiment operation is convenient.
Drawings
FIG. 1 is a schematic structural diagram of a micro-droplet generation and collection reaction system according to a first embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a micro-droplet generator according to a first embodiment of the present invention;
FIG. 3 is a schematic structural view of a micro droplet collecting reactor according to a first embodiment of the present invention;
FIG. 4 is a schematic structural view of a micro droplet collecting reactor according to a second embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail below with reference to the accompanying drawings. However, it will be appreciated by those of ordinary skill in the art that numerous technical details are set forth in order to provide a better understanding of the present application in various embodiments of the present invention. However, the technical solution claimed in the present application can be implemented without these technical details and various changes and modifications based on the following embodiments.
A first embodiment of the present invention relates to a micro-droplet generation and collection reaction system, as shown in fig. 1, comprising: a micro-droplet generator 1, a micro-droplet collecting reactor 2 and a connecting pipeline 3.
The micro-droplet generator 1 comprises a first liquid inlet channel 11, a second liquid inlet channel 12 and a micro-droplet output channel 13 which are intersected at one point, a first liquid inlet 14 communicated with the first liquid inlet channel 11, a second liquid inlet 15 communicated with the second liquid inlet channel 12, a micro-droplet outlet 16 communicated with the micro-droplet output channel 13, a first liquid inlet interface 17 arranged at the first liquid inlet 14, a second liquid inlet interface 18 arranged at the second liquid inlet 15 and a micro-droplet output interface 19 arranged at the micro-droplet outlet 16.
Specifically, the first liquid inlet channel 11, the second liquid inlet channel 12 and the micro-droplet output channel 13 all meet at the tail end, the first liquid inlet 14 is located at the head end of the first liquid inlet channel 11, the second liquid inlet 15 is located at the head end of the second liquid inlet channel 12, and the micro-droplet outlet 16 is located at the head end of the micro-droplet output channel 13.
The micro-droplet collecting reactor 2 comprises a containing cavity 21 for collecting micro-droplets, a micro-droplet inlet 22 communicated with the containing cavity 21, and a micro-droplet input interface 23 arranged at the micro-droplet inlet 22.
The connecting pipeline 3 is a flexible pipeline, is bent to be in an inverted U shape, is detachably connected to the micro-droplet output interface 19 and the micro-droplet input interface 23, and is used for communicating the micro-droplet outlet 16 with the micro-droplet inlet 22.
The first liquid inlet interface 17, the second liquid inlet interface 18, the micro-droplet output interface 19 and the micro-droplet input interface 23 are all flexible interfaces, are tubular, are made of flexible materials, have certain elastic deformation capacity, and can be directly inserted into each interface when the end part of a connecting part in a micro-droplet generation and collection reaction system has certain hardness, so that communication is realized, and the operation is simple and convenient.
When the micro-droplet liquid inlet device is used, a first-phase liquid is conveyed into the first liquid inlet 14 through the first liquid inlet interface 17, a second-phase liquid is conveyed into the second liquid inlet 15 through the second liquid inlet interface 18, the flow shearing force and the surface tension of the two mutually insoluble two-phase liquids interact through the first liquid inlet channel 11, the second liquid inlet channel 12 and the micro-droplet output channel 13 which are intersected at one point, and the produced micro-droplets enter the micro-droplet output channel 13, sequentially pass through the micro-droplet output interface 19, the connecting pipeline 3 and the micro-droplet input interface 23 and finally enter the accommodating cavity 21. Moreover, the connecting pipeline 3 is detachably connected to the micro-droplet output interface 19 and the micro-droplet input interface 23, so that, compared with the prior art, the micro-droplet generator 1 and the micro-droplet collection reactor 2 can be separated and respectively manufactured by selecting materials, and micro-droplets generated by the micro-droplet generator 1 can be directly collected and reacted.
It should be noted that the micro droplet collecting reactor 2 in this embodiment further includes an exhaust port 24 communicating with the accommodating cavity 21, and an exhaust port 25 disposed at the exhaust port 24, and when the micro droplets enter the accommodating cavity 21, the exhaust port 25 can be opened to exhaust the air remaining in the accommodating cavity 21 to the outside, so as to fill the entire accommodating cavity 21 with the micro droplets and avoid the generation of bubbles.
As shown in fig. 2, the micro-droplet generator 1 of the present embodiment includes a generator cover 4 and a generator base 5, and the generator cover 4 includes a first liquid inlet channel 41, a second liquid inlet channel 42, and a micro-droplet outlet channel 43 which are joined at one point. Meanwhile, the first liquid inlet 14, the second liquid inlet 15 and the micro-droplet outlet 16 are respectively arranged on the generator cover plate 4 in a penetrating manner, and the first liquid inlet 14 is communicated with the first liquid inlet channel 41, the second liquid inlet 15 is communicated with the second liquid inlet channel 42, and the micro-droplet outlet 16 is communicated with the micro-droplet output channel 43. The generator cover plate 4 and the generator substrate 5 are connected with each other, the first liquid inlet channel 41 and the generator substrate 5 form a first liquid inlet channel 11, the second liquid inlet channel 42 and the generator substrate 5 form a second liquid inlet channel 12, and the micro-droplet output channel 43 and the generator substrate 5 form a micro-droplet output channel 13. The adhesive connection between the generator cover plate 4 and the generator base plate 5 can be realized by means of double-sided adhesive or other adhesive means.
The bottoms of the first liquid inlet interface 17, the second liquid inlet interface 18 and the micro-droplet output interface 19 and the surface of the generator cover plate 4 are smooth planes, and the first liquid inlet interface, the second liquid inlet interface 18 and the micro-droplet output interface are respectively bonded on the generator cover plate 4. In summary, the manufacturing of the micro-droplet generator 1 is simplified by the splicing combination structure.
The generator cover plate 4 and/or the generator base plate 5 are made of an inorganic material or a polymer material, i.e. the generator cover plate 4 and/or the generator base plate 5 can be made of different materials according to the actual needs, without any restriction. Wherein the inorganic material comprises: silicon, glass, quartz glass, etc.; the polymer material includes PDMS (polydimethylsiloxane), PMMA (polymethyl methacrylate), PS (polystyrene), PC (polycarbonate), COC/COP (cyclic olefin copolymer), and the like, especially PDMS and PMMA.
In addition, it is worth to be noted that, as shown in fig. 3, the micro droplet collecting reactor 2 in the present embodiment includes a collecting reactor cover plate 6 and a collecting reactor base plate 7, the collecting reactor cover plate 6 is provided with an accommodating cavity groove 61; the micro-droplet inlets 22 are penetratingly provided on the collecting reactor cover plate 6 and communicate with the receiving chamber recess 61. In particular, in the present embodiment, since the height of the housing chamber 21 is H and the diameter of the micro-droplet output from the micro-droplet generator 1 is H, 0.5H < 2H, the micro-droplet can exist only in one layer in the housing chamber 21, which is convenient for the subsequent experimental operation.
Similarly, the collecting reactor cover plate 6 and the collecting reactor base plate 7 are connected to each other, and the receiving chamber recess 61 and the collecting reactor base plate 7 form the receiving chamber 21. The micro-droplet input interface 23 is adhered to the collecting reactor cover plate 6, and the manufacturing of the micro-droplet collecting reactor 2 is simplified through the splicing combination structure.
In the present embodiment, the exhaust port 24 is also provided in the collecting reactor cover plate 6, and the exhaust port 25 is bonded to the collecting reactor cover plate 6. When in use, a valve can be connected to the exhaust port 25 to control the opening and closing of the exhaust port 24.
In the present embodiment, the collection reactor cover plate 6 and/or the collection reactor base plate 7 are made of an inorganic material, and prevent phenomena such as volatilization of a liquid phase and coalescence and breakage of fine droplets. Of course, the collecting reactor cover plate 6 and/or the collecting reactor base plate 7 may also be made of a hard polymer material with a low thermal expansion capacity, such as PC, PS, PMMA, etc.
A second embodiment of the present invention relates to a micro-droplet generation and collection reaction system. The second embodiment is substantially the same as the first embodiment, and mainly differs therefrom in that: in a first embodiment, a micro droplet collection reactor comprises a collection reactor cover plate and a collection reactor base plate. In the second embodiment of the present invention, as shown in fig. 4, the micro droplet collecting reactor 2 comprises a collecting reactor top plate 8, a collecting reactor partition plate 9 and a collecting reactor bottom plate 10; the micro-droplet inlet 22 is penetratingly arranged on the collection reactor top plate 8; the collecting reactor partition plate 9 is provided with an accommodating cavity through hole 91; collect reactor roof 8, collect reactor baffle 9 and collect reactor bottom plate 10 and connect gradually, collect reactor baffle 9 and be located and collect between reactor roof 8 and the collection reactor bottom plate 10, collect reactor roof 8, hold chamber through-hole 91 and collect reactor bottom plate 10 and form and hold the chamber, little liquid drop input interface 23 bonds on collecting reactor roof 8. In conclusion, the fabrication of the micro droplet collecting reactor 2 is simplified by the splicing combination structure.
In the present embodiment, the exhaust port 24 is also provided in the collection reactor ceiling 8, and the exhaust port 25 is bonded to the collection reactor ceiling 8. When in use, a valve can be connected to the exhaust port 25 to control the opening and closing of the exhaust port 24.
It will also be appreciated that the collection reactor ceiling 8 and/or the collection reactor floor 10 in this embodiment are made of inorganic materials to prevent liquid phase evaporation, micro-droplet coalescence fracture, and the like. Of course, the collecting reactor ceiling 8 and/or the collecting reactor floor 10 may also be made of a hard polymer material with a low thermal expansion capacity, such as PC, PS, PMMA, etc. In addition, it should be noted that the collecting reactor partition 9 is made of a colloidal material and has viscosity so that the collecting reactor partition 9 can be directly bonded to the collecting reactor upper plate 8 and the collecting reactor lower plate 10.
It should also be noted that the thickness of the collecting reactor partition 9 is the height H of the receiving chamber, so that the thickness of the collecting reactor partition 9 also needs to satisfy 0.5H < H < 2H.
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples for carrying out the invention, and that various changes in form and details may be made therein without departing from the spirit and scope of the invention in practice.
Claims (7)
1. A micro-droplet generation and collection reaction system, comprising: the device comprises a micro-droplet generator, a micro-droplet collecting reactor and a connecting pipeline;
the micro-droplet generator comprises a first liquid inlet channel, a second liquid inlet channel, a micro-droplet output channel, a first liquid inlet, a second liquid inlet, a micro-droplet outlet, a first liquid inlet interface, a second liquid inlet interface and a micro-droplet output interface, wherein the first liquid inlet channel, the second liquid inlet channel and the micro-droplet output channel are intersected at one point;
the micro-droplet collection reactor comprises a containing cavity for collecting micro-droplets, a micro-droplet inlet communicated with the containing cavity, and a micro-droplet input interface arranged at the micro-droplet inlet;
the connecting pipeline is detachably connected to the micro-droplet output interface and the micro-droplet input interface and is used for communicating the micro-droplet outlet with the micro-droplet inlet;
the micro-droplet generator comprises a generator cover plate and a generator substrate, the first liquid inlet interface, the second liquid inlet interface and the micro-droplet output interface are respectively bonded on the generator cover plate, and the first liquid inlet interface, the second liquid inlet interface, the micro-droplet output interface and the micro-droplet input interface are all flexible interfaces;
the height of the accommodating cavity is H, the diameter of the micro liquid drop output by the micro liquid drop generator is H, and H is more than 0.5H and less than 2H;
the micro-droplet collection reactor comprises a collection reactor cover plate and a collection reactor base plate, wherein the collection reactor cover plate is provided with an accommodating cavity groove;
the micro-droplet inlet is penetratingly arranged on the collecting reactor cover plate and is communicated with the accommodating cavity groove;
the collecting reactor cover plate and the collecting reactor base plate are connected with each other, and the accommodating cavity groove and the collecting reactor base plate form the accommodating cavity;
the micro-droplet input interface is adhered to the collection reactor cover plate;
or; the micro-droplet collection reactor comprises a collection reactor top plate, a collection reactor partition plate and a collection reactor bottom plate;
the micro-droplet inlet is penetratingly arranged on the top plate of the collection reactor;
the collecting reactor partition plate is provided with an accommodating cavity through hole;
the collecting reactor top plate, the collecting reactor partition plate and the collecting reactor bottom plate are sequentially connected, and the collecting reactor top plate, the accommodating cavity through hole and the collecting reactor bottom plate form the accommodating cavity.
2. The system of claim 1, wherein the micro-droplet collection reactor further comprises an exhaust port in communication with the receiving chamber, and an exhaust port disposed at the exhaust port.
3. The micro-droplet generation and collection reaction system of claim 1, wherein the generator cover plate is provided with a first inlet channel, a second inlet channel and a micro-droplet output channel which meet at a point;
the first liquid inlet, the second liquid inlet and the micro-droplet outlet are respectively arranged on the generator cover plate in a penetrating manner, the first liquid inlet is communicated with the first liquid inlet concave channel, the second liquid inlet is communicated with the second liquid inlet concave channel, and the micro-droplet outlet is communicated with the micro-droplet output concave channel;
the generator cover plate is connected with the generator substrate, the first liquid inlet channel and the generator substrate form the first liquid inlet channel, the second liquid inlet channel and the generator substrate form the second liquid inlet channel, and the micro-droplet output channel and the generator substrate form the micro-droplet output channel;
the first liquid inlet interface, the second liquid inlet interface and the micro-droplet output interface are respectively bonded on the generator cover plate.
4. The micro-droplet generation and collection reaction system of claim 3, wherein the generator cover plate and/or the generator substrate are made of an inorganic material or a polymer material.
5. The micro-droplet generation and collection reaction system of claim 1, wherein the collection reactor cover plate and/or the collection reactor base plate are made of an inorganic material and/or a rigid polymeric material.
6. The micro-droplet generation and collection reaction system of claim 1, wherein the collection reactor ceiling and/or the collection reactor floor are made of an inorganic material and/or a rigid polymeric material.
7. The micro-droplet generation and collection reaction system of claim 6, wherein the collection reactor partition is made of a colloidal material.
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| CN114223650A (en) * | 2021-12-29 | 2022-03-25 | 序康医疗科技（苏州）有限公司 | Cryopreservation method and device for biological material |
| CN115254216A (en) * | 2022-07-21 | 2022-11-01 | 苏州索真生物技术有限公司 | Method for processing micro-fluidic chip |
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| CN105170207A (en) * | 2015-09-29 | 2015-12-23 | 北京工业大学 | Micro-droplet control chip based on branch structure |
| CN204952875U (en) * | 2015-09-30 | 2016-01-13 | 天津广顺化学科技有限公司 | Two control by temperature change microchannels reaction system |
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| KR100540143B1 (en) * | 2003-12-22 | 2006-01-10 | 한국전자통신연구원 | Microfluidic control device and method for controlling microfluidic |
| KR100942184B1 (en) * | 2008-04-25 | 2010-02-11 | 한국과학기술연구원 | Apparatus and method for fabricating micro-capsule |
| CN204816577U (en) * | 2015-07-30 | 2015-12-02 | 天津大学 | Novel microbubble / liquid drop generates regulation and control device |
| CN105567560A (en) * | 2015-12-30 | 2016-05-11 | 西安交通大学 | Integrated liquid drop microfluidic chip |
| CN205874440U (en) * | 2016-04-08 | 2017-01-11 | 周辉 | Ribonucleic chain polymerization amplification reaction detection device |
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| CN105170207A (en) * | 2015-09-29 | 2015-12-23 | 北京工业大学 | Micro-droplet control chip based on branch structure |
| CN204952875U (en) * | 2015-09-30 | 2016-01-13 | 天津广顺化学科技有限公司 | Two control by temperature change microchannels reaction system |
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