CN205874440U - Ribonucleic chain polymerization amplification reaction detection device - Google Patents
Ribonucleic chain polymerization amplification reaction detection device Download PDFInfo
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- CN205874440U CN205874440U CN201620816574.0U CN201620816574U CN205874440U CN 205874440 U CN205874440 U CN 205874440U CN 201620816574 U CN201620816574 U CN 201620816574U CN 205874440 U CN205874440 U CN 205874440U
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- 238000001514 detection method Methods 0.000 title claims abstract description 46
- 238000006116 polymerization reaction Methods 0.000 title claims abstract description 43
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 27
- 230000003321 amplification Effects 0.000 title abstract description 9
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 239000002699 waste material Substances 0.000 claims description 25
- 238000001917 fluorescence detection Methods 0.000 claims description 18
- 229920002477 rna polymer Polymers 0.000 claims description 17
- 238000004458 analytical method Methods 0.000 claims description 15
- 238000012408 PCR amplification Methods 0.000 claims description 7
- 238000007689 inspection Methods 0.000 claims description 7
- 238000007493 shaping process Methods 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000005842 biochemical reaction Methods 0.000 abstract description 3
- 230000029918 bioluminescence Effects 0.000 abstract 1
- 238000005415 bioluminescence Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 24
- 238000003752 polymerase chain reaction Methods 0.000 description 17
- 108020004414 DNA Proteins 0.000 description 15
- 238000000034 method Methods 0.000 description 14
- 238000005516 engineering process Methods 0.000 description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 5
- 230000004544 DNA amplification Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 238000001506 fluorescence spectroscopy Methods 0.000 description 3
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- 238000012360 testing method Methods 0.000 description 3
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- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 2
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- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 241000018646 Pinus brutia Species 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
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- 239000007850 fluorescent dye Substances 0.000 description 2
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- 150000007523 nucleic acids Chemical group 0.000 description 2
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- 238000003786 synthesis reaction Methods 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 1
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- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
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- 239000002342 ribonucleoside Substances 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/143—Quality control, feedback systems
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/143—Quality control, feedback systems
- B01L2200/147—Employing temperature sensors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Clinical Laboratory Science (AREA)
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- Genetics & Genomics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a ribonucleic chain polymerization amplification reaction detection device sets up by the little liquid drop generation system of ultrasonic vibration system's driven, set up a plurality of application of sample holes on little liquid drop generation system, the ultrasonic vibration system produces the vibration, makes PCR sample and oil among little liquid drop generation system drip to mix and form an oil lenses and wrap up in and get into the polymerization system behind little liquid drop. Adopt above -mentioned technical scheme, make the reading of production, polymerization and glimmering optical data of little liquid drop go on on an equipment, avoided losing of sample, it is convenient, accurate, swift, can effective satisfy and carry out external enlarged temperature gradient's requirement to DNA, show the speed that improves biochemical reaction, both save time, raise the efficiency, the batchization of being convenient for again, low cost production, realize that the micro -fluidic bioluminescence of PCR increase circulation carries out real -time fluorescence information feedback, automatic more, miniaturation have shortened the duty cycle of system, sample signal acquisition's loss has been avoided.
Description
Technical field
This utility model belongs to the technical field of biological engineering experimental instrument and equipment, relates to belonging to the amplification of PCR reagent, glimmering
Photo measure detection technique, more specifically, this utility model relates to the data of ribonucleic acid chain type polymerization amplified reaction and processes one
The detection device of body.
Background technology
Round pcr (DNA Polymerase Chain Reaction:DNA polymerase chain reaction) is the mid-80
The isothermal DNA amplification grown up, is that Khorana proposed this imagination in 1971 the earliest.American scientist Kary
Mullis etc. achieved this imagination in 1985, had invented the round pcr being with historically new significance.Apply this technology can be
In one test tube, genes of interest to be studied was expanded within a few hours 100,000 or even million times, it is simple to naked eyes can directly be seen
Examine and judge.Due to this isothermal DNA amplification have special, sensitive, productivity is high, quick, easy, reproducible, easily from
The outstanding advantages such as dynamicization, have therefore obtained the generally accreditation of life sciences circle, and the most therefore Kary Mullis obtains 1993 years
Nobel chemistry Prize.
PCR is one of the most frequently used molecular biology method.Removing in the field of basic scientific research, PCR examines at clinical medicine
Disconnected, life sciences, engineering in medicine, genetics, agricultural seed selection educate clock research and development, and criminal calibrating screen in be all essential
, also it is the most authoritative reliable technology.
Archaeal dna polymerase chain reaction (PCR): be the technology being carried out DNA amplification by thermal cycle reaction.Thermal cycle refers to solid
The circulation of variations in temperature in fixed time interval.Using heat-staple archaeal dna polymerase, PCR can be de-with the synthesis material of DNA
Oxygen ribonucleoside triphosphote synthesizes substantial amounts of DNA copy.PCR reaction includes three steps: degeneration, anneals and extends.Degeneration is PCR cycle
The first step, the hydrogen bond between DNA base is opened and is melted DNA double chain and produce single stranded DNA by it.Annealing then reduces the temperature to
Of a sufficiently low, enable oligonucleotide primer to be attached on DNA profiling.During extending, archaeal dna polymerase is by new for synthesis double
Chain DNA.After repeatedly thermal cycle (each cyclic amplification one times), the DNA of trace will be expanded to the concentration of instrument detection.
Common PCR detection technique has two kinds:
After the first is multi cycle polyreaction, end-product (end-point analysis) is detected by DNA running gel
Quantitatively.Shortcoming is because being limited by electrophoretic techniques sensitivity, and this method is simply " sxemiquantitative ".
Second method is: utilizes fluorescently-labeled probe (fluorescent probes), monitors polyreaction in real time
Change of production in each circulation.The quantity of initial template to be expanded can be calculated by cycle threshold (Ct) value and draw.
Ct value is exactly the period when fluorescent value is apparently higher than background background.
The real-time PCR reagent sold in the market is mainly based upon the TaqMan of fluorescent probe and based on SYBR
Green dyestuff is fitted together to the two kinds of technology surveying DNA double chain total amount.The limitation of the method is: 1, need external source standard sample or
Person's endogenous gene carrys out standardization to paint calibration directrix curve.2, the amplification that non-specific responding causes will affect the credibility of Ct value.
Research worker finds that following three factors and polyreaction success or not have a dependency: 1, the limiting dilution of sample;
2, end-product total amount;3, product amount is distributed (Poisson distribution) relation with the handkerchief pine of initial DNA concentration.
The concentration of specimens of initial DNA just can be extrapolated by regulating and controlling and measuring the above-mentioned factor.
Utility model content
This utility model provides the data of a kind of ribonucleic acid chain type polymerization amplified reaction to process detecting device integrated, its
Purpose is to realize the convenient, quick of its detection method and accurately.
To achieve these goals, the technical scheme that this utility model is taked is:
Ribonucleic acid chain type of the present utility model polymerization amplified reaction detection device, including polymerization reaction system, described
Detection device arranges microlayer model and produces system, and described microlayer model produces system and driven by ultrasonic vibration system;Described is micro-
Multiple well is set on droplet-generating systems;Described ultrasonic vibration system produces vibration, makes described microlayer model produce
PR sample in system and oil droplet enter described polymerization reaction system after being mixed to form oil parcel microlayer model.
The specification of all described wells is consistent, and each described well is equipped with oil depot, sample library and micro-liquid
Drip storehouse.Described oil depot, sample library and microlayer model storehouse are cylindrical shape, are connected by pipeline between three.
Described detection device arranges intelligent temperature control system;Described intelligent temperature control system is to described polymerization reaction system
Temperature control in real time.
Described intelligent temperature control system includes that Temperature sampler, temperature sensor, single-chip microcomputer, multiway analog switch, A/D turn
Parallel operation, D/A converter and display.Described Temperature sampler, temperature sensor are integrated into a chip fixture apparatus;Described
Single-chip microcomputer be connected with A/D converter, D/A converter and display by signal line;Described A/D converter and multichannel mould
Intend switch to be connected by signal line;Described D/A converter is connected by signal line with drive circuit;Described multichannel mould
Intend switch and drive circuit is all connected with chip fixture apparatus.
Described detection device arranges laser transmitting system;Described laser transmitting system comprises LASER Light Source and excites
Light shaping device;Described exciting light reshaper makes the line source sent from described LASER Light Source through described exciting light shaping
Present an area source after device shaping, the PR sample of described polymerization reaction system is irradiated.
Described detection device arranges fluorescence detection acquisition system;Described fluorescence detection acquisition system comprises and microlayer model
The detector of the identical hole count of generation system;The microchannel of described detector detection direction and described polymerization reaction system is one by one
Corresponding.
Described detection device arranges Signal Analysis System, and described fluorescence detection acquisition system detects the fluorescence collected
Signal sends described Signal Analysis System to
Described fluorescence detection acquisition system comprises optical lens, optical filter and optical-electrical converter.
Described detection device arranges waste liquid and discharges system;It is tubulose that described waste liquid discharges system, after reaction being terminated
All waste liquids get rid of to waste liquid box.
Described polymerization reaction system includes microchannel row, heater strip;Described heater strip is respectively placed in different microchannel
The bottom of row;Described polymerization reaction system was controlled to carry out PR amplification in different temperature ranges by the setting time.
In order to realize the goal of the invention identical with technique scheme, this utility model additionally provides above-described ribose
The data of nucleic acid chain polymerization amplified reaction process detecting device integrated detection method, and its technical scheme is:
Oil droplet and PR sample being added microlayer model and produces system, open ultrasonic vibration system, microlayer model produces system and opens
Begin to vibrate, make oil droplet and PR sample form oil parcel drop and enter polymerization reaction system;
Being then turned on intelligent temperature control system, intelligent temperature control system makes the temperature of polymerization reaction system control by the setting time
Different warm areas carries out PR amplification;Open laser transmitting system and fluorescence detection acquisition system simultaneously, Signal Analysis System enter
Row real time data reads and analyzes;
After experiment terminates, open the valve of polyreaction, make waste liquid discharge system from waste liquid and flow out, enter waste liquid box.
This utility model uses technique scheme, makes the reading of the generation of microlayer model, polyreaction and fluorescence data
An equipment carries out, it is to avoid the loss of sample, convenient, accurately, quick;Three warm areas can be carried out solely by attemperating unit
Erect and put, control and display in real time, can effectively meet the requirement of the thermograde that DNA is carried out external amplification;It is easy and fast to reality
Existing PCR amplification, significantly improves the speed of biochemical reaction, the most time-consuming, improve efficiency, is easy to again mass, low one-tenth
This production;Realize PCR amplification cycles micro-fluidic biological fluorescence and carry out real-time fluorescence information feedback;Decrease ancillary equipment, more
Automatization, miniaturization, shorten system duty cycle;Avoid the loss that sample signal gathers.
Accompanying drawing explanation
In accompanying drawing content and figure, labelling is briefly described as follows:
Fig. 1 is that the data of microlayer model flow model high flux ribonucleic acid chain type of the present utility model polymerization amplified reaction process
Detecting device integrated structural representation;
Fig. 2 is the well schematic diagram in Fig. 1;
Fig. 3 is that the microchannel in Fig. 1 lists intention;
Fig. 4 is the exciting light reshaper schematic diagram in Fig. 1;
Fig. 5 is the LASER Light Source schematic diagram in Fig. 1;
Fig. 6 is the structural representation of the intelligent temperature control system in this utility model.
Being labeled as in figure:
A, microlayer model produce system, B, ultrasonic vibration system, C, polymerization reaction system, D, intelligent temperature control system, E, swash
Light emission system, F, fluorescence detection acquisition system, G, valve, H, waste liquid discharge system, L, Signal Analysis System, M, waste liquid box,
A, well, b, microchannel row, c, exciting light reshaper, d, LASER Light Source, 1, oil depot, 2, sample library, 3, microlayer model storehouse, 4, micro-
Pipeline, 5, short focal length lens, 6, long-focus lens, 7, Laser Power Devices, 8, laser instrument, 9, fiber coupler.
Detailed description of the invention
Below against accompanying drawing, by the description to embodiment, detailed description of the invention of the present utility model is made the most in detail
Thin explanation, with help those skilled in the art inventive concept of the present utility model, technical scheme are had more complete, accurately and
Deep understanding.
As it was previously stated, have the factor of dependency to include with polyreaction success or not: 1, the limiting dilution of sample;2, eventually
Product total amount;3, product amount is distributed (Poisson distribution) relation with the handkerchief pine of initial DNA concentration.By regulation and control and
Measure the above-mentioned factor and just can extrapolate the concentration of specimens of initial DNA.
Based on this principle, this utility model provides structure as shown in Figure 1, for a kind of microlayer model flow model high flux
Ribonucleic acid chain type polymerization amplified reaction detection device, is widely used in molecular biology, heredity and medical science.This inspection
Survey device and include polymerization reaction system C.This detection device relates to microlayer model and produces system, ultrasonic vibration system, polyreaction
The aspects such as system, intelligent temperature control system, fluorescence detection acquisition analysis system and waste liquid discharge system.This device contains biological core
Chip technology, biology information technology, computer hardware technique and microflow control technique.
In order to overcome the defect of prior art, it is achieved the detection method of ribonucleic acid chain type polymerization amplified reaction detection device
Convenience, goal of the invention accurate, quick, the technical scheme that this utility model is taked is:
As it is shown in figure 1, ribonucleic acid chain type of the present utility model polymerization amplified reaction detection device, described detection device
Arranging microlayer model and produce system A, described microlayer model produces system A and is driven by ultrasonic vibration system B;Described microlayer model produces
In raw system A, multiple well a is set;Described ultrasonic vibration system B produces vibration, makes described microlayer model produce system
PCR sample in A and oil droplet enter described polymerization reaction system C after being mixed to form oil parcel microlayer model.
The structure of well a is as shown in Figure 2.
Ultrasonic vibration system B utilizes the high frequency sound wave of ultrasound wave to produce vibration.In fact, this device is not limited to ultrasound wave and shakes
Move and also comprise the various mode of vibrations such as mechanical vibration.
Microlayer model produces system A and is provided with 256 wells.Well a in system can be 256, it is also possible to is
The varying numbers such as 96 holes.
The specification of all described well a is consistent, and each described well a is equipped with oil depot 1, sample library 2 and
Microlayer model storehouse 3.Described oil depot 1, sample library 2 and microlayer model storehouse 3 are cylindrical shape, are connected by pipeline between three.
Pipeline is curve shape, and liquid is when pipe vibration, and drop, in internal flow, produces microlayer model.
Well a is divided into oil depot and loading slot, in tapered, when microlayer model produces system vibration, in PCR sample and oil depot
Oil mix, produce oil parcel microlayer model.
As shown in Figure 6, described detection device arranges intelligent temperature control system D;Described intelligent temperature control system D is to described
The temperature of polymerization reaction system C controls in real time.
Described intelligent temperature control system D includes Temperature sampler, temperature sensor, single-chip microcomputer, multiway analog switch, A/D
Transducer, D/A converter and display.
Described Temperature sampler, temperature sensor are integrated into a chip fixture apparatus;Described single-chip microcomputer is by letter
Number circuit is connected with A/D converter, D/A converter and display;Described A/D converter and multiway analog switch pass through signal
Connection;Described D/A converter is connected by signal line with drive circuit;Described multiway analog switch and driving electricity
Lu Junyu chip fixture apparatus connects.
The temperature of polymerization reaction system C is controlled by intelligent temperature control system D in real time.
Described detection device arranges laser transmitting system E;Described laser transmitting system E comprise LASER Light Source d and
Exciting light reshaper c;Described exciting light reshaper c makes the line source sent from described LASER Light Source d swash through described
Present an area source after luminous reshaper c shaping, the PCR sample of described polymerization reaction system C is irradiated.
The structure of exciting light reshaper c is as shown in Figure 4.The structure of LASER Light Source d is as shown in Figure 5.
Microchannel 4 microchannel be blood capillary fill fluid passage, short focal length lens 5 before long-focus lens 6, the two
Between distance be the two focal length sum;Laser instrument 8 is before short focal length lens 5, and fiber coupler 9 is at short focal length lens
Between 5 and laser instrument 8.Laser beam is coupled after sending laser by laser instrument 8 by fiber coupler 9, then by short Jiao
Expand away from lens 5 and long-focus lens 6.
Laser Power Devices 7 provide power supply for laser instrument 8.
One point-like luminous source that can send continuous print visible ray, if its light only becomes to a direction in space
Linear propagation, then this luminous point is thus referred to as line source.
Area source is one face of one-tenth rather than a line after light beam output.
Described detection device arranges fluorescence detection acquisition system F;Described fluorescence detection acquisition system F comprises and micro-liquid
Drip the detector of the identical hole count of generation system A;Described detector detection direction and the microchannel of described polymerization reaction system C
One_to_one corresponding.
Fluorescence detection equipment is a multi-body system being made up of circuit, light path, and each system coordination coordinates realization steady
The basis that regular inspection is surveyed, is the optical detection apparatus of comparative maturity.
Described fluorescence detection acquisition system F comprises optical lens, optical filter and optical-electrical converter.
Described detection device arranges Signal Analysis System L, and it is glimmering that described fluorescence detection acquisition system F detection collects
Optical signal sends described Signal Analysis System L to
Described detection device arranges waste liquid and discharges system H;It is tubulose that described waste liquid discharges system H, reaction is terminated
After all waste liquids get rid of to waste liquid box M.
Described polymerization reaction system C includes microchannel row b, heater strip ambroin;Described heater strip is respectively placed in
The bottom of different microchannel row b;Described polymerization reaction system C temperature was controlled to carry out in different temperature ranges by the setting time
PCR expands.
The structure of microchannel row b is as shown in Figure 3.Including multiple microchannels 4.
Microchannel row are that blood capillary fills fluid passage, and heater strip is curved metal silk, and heater strip is under microchannel
Face.
In sum, this utility model includes: microlayer model produces system A, ultrasonic vibration system B, polyreaction system
System C, intelligent temperature control system D, laser transmitting system D, fluorescence detection acquisition system F, Signal Analysis System L and waste liquid discharge system
H。
The utility model has the advantages that:
1, the reading of the generation of microlayer model, polyreaction and fluorescence data is carried out on one device, it is to avoid sample
Loss, convenient, accurately, quick;
2, three warm areas can be independently arranged by attemperating unit, controls and display in real time, can effectively meet to enter DNA
The requirement of the thermograde of the external amplification of row;
3, it is easy and fast to realize PCR amplification, significantly improves the speed of biochemical reaction, the most time-consuming improve again effect
Rate, it is simple to mass, low-cost production;
4, achieve PCR amplification cycles micro-fluidic biological fluorescence and carry out real-time fluorescence information feedback;
5, decreasing ancillary equipment, more automatization, miniaturization, shorten system duty cycle;
6, the loss that sample signal gathers is avoided.
In order to realize the goal of the invention identical with technique scheme, this utility model additionally provides above-described ribose
The detection method of nucleic acid chain polymerization amplified reaction detection device, its technical scheme is:
Oil droplet and PCR sample being added microlayer model and produces system A, open ultrasonic vibration system B, microlayer model produces system
A starts vibration, makes oil droplet and PCR sample form oil parcel drop and enter polymerization reaction system C;
Being then turned on intelligent temperature control system D, intelligent temperature control system D makes the temperature of polymerization reaction system C control by the setting time
System carries out PCR amplification at different warm areas;Open laser transmitting system E and fluorescence detection acquisition system F, by signal analysis simultaneously
System L carries out real time data reading and analysis;
After experiment terminates, open the valve G of polyreaction, make waste liquid discharge system H from waste liquid and flow out, enter waste liquid box M.
Scheme as described above, specific implementation of the present utility model is: the microlayer model in described detection device
There are 256 wells in generation system, utilize the high frequency sound wave of ultrasound wave to produce vibration, make the PCR in microlayer model generation system
Sample and oil droplet enter polymerization reaction system after being mixed to form oil parcel microlayer model;Polymerization reaction system is made by temperature control system
Temperature was controlled to carry out PCR amplification at different warm areas (94 DEG C, 55 DEG C, 72 DEG C) by the setting time;Laser transmitting system uses
It it is ultraviolet excitation;The fluorescence signal of sample is collected, analyzes and processes by fluorescence detection acquisition analysis system;After experiment terminates
Waste liquid is discharged system from waste liquid and is flowed out.
Test result indicate that: whole device can realize the reading analysis of the generation of microlayer model, polyreaction, fluorescence data
And a series of functions such as waste liquid discharge, test convenient, fast.
Above in conjunction with accompanying drawing, this utility model is exemplarily described, it is clear that this utility model implements and is not subject to
The restriction of aforesaid way, as long as have employed changing of the various unsubstantialities that method of the present utility model is conceived and technical scheme is carried out
Enter, or the most improved design of the present utility model and technical scheme are directly applied to other occasion, all at this utility model
Protection domain within.
Claims (9)
1. a ribonucleic acid chain type polymerization amplified reaction detection device, including polymerization reaction system (C), it is characterised in that: institute
The detection device stated arranges microlayer model and produces system (A), and described microlayer model produces system (A) by ultrasonic vibration system (B)
Drive;Described microlayer model produces and arranges multiple well (a) in system (A);Described ultrasonic vibration system (B) produces shakes
Swing, described in the PCR sample making described microlayer model produce in system (A) enters after being mixed to form oil parcel microlayer model with oil droplet
Polymerization reaction system (C).
2. it is polymerized amplified reaction detection device according to the ribonucleic acid chain type described in claim 1, it is characterised in that: each described
Well (a) be equipped with oil depot (1), sample library (2) and microlayer model storehouse (3);Described oil depot (1), sample library (2) and micro-liquid
Drip storehouse (3) and be cylindrical shape, connected by pipeline between three.
3. it is polymerized amplified reaction detection device according to the ribonucleic acid chain type described in claim 1, it is characterised in that: described inspection
Survey device and intelligent temperature control system (D) is set;The described intelligent temperature control system (D) temperature to described polymerization reaction system (C)
Control in real time.
4. it is polymerized amplified reaction detection device according to the ribonucleic acid chain type described in claim 3, it is characterised in that: described intelligence
Temperature sampler, temperature sensor, single-chip microcomputer, multiway analog switch, A/D converter, D/A conversion can be included by temperature control system (D)
Device and display;Described Temperature sampler, temperature sensor are integrated into a chip fixture apparatus;Described single-chip microcomputer passes through
Signal line is connected with A/D converter, D/A converter and display;Described A/D converter and multiway analog switch are by letter
Number connection;Described D/A converter is connected by signal line with drive circuit;Described multiway analog switch and driving
Circuit is all connected with chip fixture apparatus.
5. it is polymerized amplified reaction detection device according to the ribonucleic acid chain type described in claim 1, it is characterised in that: described inspection
Survey device and laser transmitting system (E) is set;Described laser transmitting system (E) comprises LASER Light Source (d) and exciting light shaping
Device (c);Described exciting light reshaper (c) makes the line source sent from described LASER Light Source (d) through described exciting light
Present an area source after reshaper (c) shaping, the PCR sample of described polymerization reaction system (C) is irradiated.
6. it is polymerized amplified reaction detection device according to the ribonucleic acid chain type described in claim 1, it is characterised in that: described inspection
Survey device and fluorescence detection acquisition system (F) is set;Described fluorescence detection acquisition system (F) comprises and microlayer model generation system
(A) detector of identical hole count;One a pair, the microchannel of described detector detection direction and described polymerization reaction system (C)
Should.
7. it is polymerized amplified reaction detection device according to the ribonucleic acid chain type described in claim 6, it is characterised in that: described inspection
Surveying device and arrange Signal Analysis System (L), the fluorescence signal that described fluorescence detection acquisition system (F) detection collects sends to
Described Signal Analysis System (L)
8. it is polymerized amplified reaction detection device according to the ribonucleic acid chain type described in claim 1, it is characterised in that: described inspection
Survey device and waste liquid discharge system (H) is set;It is tubulose that described waste liquid discharges system (H), all waste liquids after reaction being terminated
Get rid of to waste liquid box (M).
9. it is polymerized amplified reaction detection device according to the ribonucleic acid chain type described in claim 1, it is characterised in that: described is poly-
Close response system (C) and include that microchannel arranges (b), heater strip;Described heater strip is respectively placed in the end of different microchannel row (b)
Portion;Described polymerization reaction system (C) was controlled to carry out PCR amplification in different temperature ranges by the setting time.
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