CN111707613B - Optical fiber mounting seat, PCR optical module and PCR instrument - Google Patents

Optical fiber mounting seat, PCR optical module and PCR instrument Download PDF

Info

Publication number
CN111707613B
CN111707613B CN202010283329.9A CN202010283329A CN111707613B CN 111707613 B CN111707613 B CN 111707613B CN 202010283329 A CN202010283329 A CN 202010283329A CN 111707613 B CN111707613 B CN 111707613B
Authority
CN
China
Prior art keywords
optical fiber
distance
length
light source
area
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202010283329.9A
Other languages
Chinese (zh)
Other versions
CN111707613A (en
Inventor
曹进涛
李冬
贺贤汉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hangzhou Bori Technology Co Ltd
Original Assignee
Hangzhou Bori Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hangzhou Bori Technology Co Ltd filed Critical Hangzhou Bori Technology Co Ltd
Priority to CN202010283329.9A priority Critical patent/CN111707613B/en
Priority to PCT/CN2020/092542 priority patent/WO2021203538A1/en
Publication of CN111707613A publication Critical patent/CN111707613A/en
Application granted granted Critical
Publication of CN111707613B publication Critical patent/CN111707613B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Optical Couplings Of Light Guides (AREA)

Abstract

The invention provides an optical fiber mounting seat, a PCR optical module and a PCR instrument, and relates to the technical field of PCR instruments, wherein the section of the optical fiber mounting seat comprises a first area and a second area, and the distance between any point in the second area and the center of the section of the optical fiber mounting seat is greater than the distance between any point in the first area and the center of the section of the optical fiber mounting seat; because the illumination intensity at the center of the light source is greater than that at the edge, the distance between the first area and the light source is far, and the distance between the second area and the light source is near, the attenuation of the light emitted by the light source before entering the first area is large, and the attenuation before entering the second area is small, so that all optical fiber bundles accessed by the optical fiber mounting seat receive uniform illumination, the problem of low detection accuracy caused by uneven illumination can be solved, and the detection performance is improved.

Description

Optical fiber mounting seat, PCR optical module and PCR instrument
Technical Field
The invention relates to the technical field of PCR instruments, in particular to an optical fiber mounting seat, a PCR optical module and a PCR instrument.
Background
Currently, optical fibers are used in many pcr (polymerase Chain reaction) gene amplifiers to guide light, and single or multiple particle LEDs are typically used as light sources. Particulate LED light sources tend to cause the emitted light to be spatially typically non-uniform due to diffraction or irregularities in emission. When the light irradiates the end faces of the plurality of optical fibers converged by the light source end, the light beams received by the optical fibers in different areas are not uniform due to position difference caused by nonuniform light output of the light source, and the phenomenon is more obvious when the multi-sample hole detection is carried out and high-flux detection is carried out by using a large number of optical fibers.
Therefore, during the detection period of the PCR gene amplification instrument, the light received by different optical fibers and the light finally irradiated into each sample hole are different, so that the detection results of samples with the same volume and the same concentration in different sample holes are different, and the accuracy of the detection results is low.
Disclosure of Invention
In view of the above, the present invention provides an optical fiber mount, a PCR optical module and a PCR instrument, so as to solve the problems of poor stability and low detection accuracy of the conventional PCR device.
In order to achieve the above purpose, the embodiment of the present invention adopts the following technical solutions:
in a first aspect, an embodiment of the present invention provides an optical fiber mount, where a center of the optical fiber mount and a center of a light source are on a same horizontal line, and the light source is configured to provide light for an optical fiber bundle; the cross section of the optical fiber installation seat comprises a first area and a second area, and the distance between any point in the second area and the center of the cross section of the optical fiber installation seat is greater than the distance between any point in the first area and the center of the cross section of the optical fiber installation seat; the optical fiber mounting seat is provided with a plurality of mounting holes, the mounting holes are uniformly distributed in the first area and the second area, the mounting holes are used for accessing optical fiber bundles, and each optical fiber bundle comprises at least one optical fiber; the distance between the end face of the optical fiber bundle accessed by the mounting hole of the first region and the light source is a first distance, the distance between the end face of the optical fiber bundle accessed by the mounting hole of the second region and the light source is a second distance, and the first distance is larger than the second distance.
In some possible embodiments, the cross-section of the fiber mount further includes a third region; the distance between any point in the third area and the center of the section of the optical fiber installation seat is larger than the distance between any point in the second area and the center of the section of the optical fiber installation seat, the distance between the end face of the optical fiber bundle accessed into the installation hole of the third area and the light source is a third distance, and the second distance is larger than the third distance.
In some possible embodiments, the mounting hole is a stepped hole comprising a first step and a second step; the first step is arranged in the mounting hole of the first area, and the second step is arranged in the mounting hole of the second area; the length difference range of the first step and the second step is 0.5-5mm, and the first step and the second step are used for enabling the first distance to be larger than the second distance.
In some possible embodiments, the stepped bore further comprises a third step, the third step being disposed within the mounting bore of the third region; the length difference range of the second step and the third step is 0.5-5mm, and the third step is used for enabling the second distance to be larger than the third distance.
In some possible embodiments, the fiber bundle extends a first length in the mounting hole of the first region, the fiber bundle extends a second length in the mounting hole of the second region, and the fiber bundle extends a third length in the mounting hole of the third region; the first length is less than the second length, and the second length is less than the third length.
In some possible embodiments, the third length differs from the second length by a value in the range of 0.5-5mm, and the second length differs from the first length by a value in the range of 0.5-5 mm.
In some possible embodiments, the side surface of the optical fiber mounting seat is provided with a plurality of screws, each screw corresponds to each mounting hole one by one, and one end of each screw, which is in contact with the optical fiber bundle, is an arc-shaped surface.
In some possible embodiments, the side of the fiber mount bearing the end face of the fiber bundle is provided with a protrusion for fixedly connecting with the filtering unit.
In a second aspect, an embodiment of the present invention provides a PCR optical module, including: a light source and the fiber mount of any of the first aspects; the center of the optical fiber mounting seat and the center of the light source are on the same horizontal line.
In a third aspect, an embodiment of the present invention provides a PCR instrument, including the PCR optical module of any one of the second aspects.
The embodiment of the invention provides an optical fiber mounting seat, a PCR optical module and a PCR instrument, wherein the cross section of the optical fiber mounting seat comprises a first area and a second area, and the distance between any point in the second area and the center of the cross section of the optical fiber mounting seat is greater than the distance between any point in the first area and the center of the cross section of the optical fiber mounting seat; because the illumination intensity at the center of the light source is greater than that at the edge, the distance between the first area and the light source is far, and the distance between the second area and the light source is near, the attenuation of the light emitted by the light source before entering the first area is large, and the attenuation before entering the second area is small, so that all optical fiber bundles accessed by the optical fiber mounting seat receive uniform illumination, the problem of low detection accuracy caused by uneven illumination can be solved, and the detection performance is improved.
In addition, the optical fiber mounting base provided by the embodiment of the invention can also fix the optical fiber bundle by using the screws on the side surface, and when the position of the optical fiber bundle is installed wrongly or the distance difference of the installed optical fibers does not accord with the range, the light intensity among different optical fibers is not uniform, the adjustment is convenient.
According to the PCR optical module provided by the embodiment of the invention, the light source adopts a surface light source, so that the light is emitted more uniformly; the part of the optical fiber mounting seat bearing the optical fiber bundle is convex, and the convex part is finally mounted on the filter wheel, so that the distance between the optical filter on the filter wheel and the optical fiber is closer and coaxially mounted, and the effect of reducing light loss is realized.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
Fig. 1 is a schematic diagram illustrating a principle of a PCR optical module according to an embodiment of the present invention;
FIG. 2 is a schematic cross-sectional view of an optical fiber mount according to an embodiment of the present invention;
FIG. 3 is a schematic view of a light source according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of an optical fiber mount according to an embodiment of the present invention;
FIG. 5 is a schematic view of another fiber mount configuration provided by an embodiment of the present invention;
FIG. 6 is a schematic view of another fiber mount configuration provided by embodiments of the present invention;
FIG. 7 is a schematic cross-sectional view of another fiber mount provided by an embodiment of the invention;
FIG. 8 is a schematic side view of an optical fiber mount according to an embodiment of the present invention;
fig. 9 is a schematic view of an installation structure of a PCR optical module according to an embodiment of the present invention.
Icon: 110-fiber mount; 210-a light source; 310-a lens; 410-a filtering unit; 111-center of cross-section of fiber mount; 112-a first area; 113-a second region; 114-mounting holes; 115-a third region; 1124-inner layer mounting holes; 1134, mounting holes on the outer layer; 1154-middle layer mounting holes; 121-outer fiber bundle; 122-inner fiber bundle; 152-middle layer fiber bundle; 123-outer layer step hole; 124-inner layer step hole; 130-a screw; 140-a bump; 501-a motor; 502-filter wheel; 503-a lens mount; 504-a connector; 505-a heat sink; 506-a fan.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Currently, optical fibers are used in many PCR gene amplifiers to guide light, and single or multiple particle LEDs are typically used as light sources. Particulate LED light sources tend to cause the emitted light to be spatially typically non-uniform due to diffraction or irregularities in emission. When the light irradiates the end faces of the plurality of optical fibers converged by the light source end, the light beams received by the optical fibers in different areas are not uniform due to position difference caused by nonuniform light output of the light source, and the phenomenon is more obvious when the multi-sample hole detection is carried out and high-flux detection is carried out by using a large number of optical fibers.
During the detection period of the PCR gene amplification instrument, the light received by different optical fibers and the light finally irradiated into each sample hole are different, so that the detection results of samples with the same volume and the same concentration in different sample holes are different, and the accuracy of the detection results is low.
Based on this, the embodiment of the invention provides an optical fiber mounting seat, a PCR optical module and a PCR instrument, so as to solve the problem of low detection accuracy caused by uneven illumination. For convenience of understanding, a detailed description will be given of an optical fiber mount according to the present invention, which is applied to the PCR optical module shown in fig. 1.
The center of the optical fiber mount 110 is on the same horizontal line as the center of the light source 210, and the light source 210 is used for providing light for the optical fiber bundle; the cross-section of the fiber mount 110 includes a first region and a second region, and in conjunction with the schematic cross-sectional view of the fiber mount shown in fig. 2, the distance between any point in the second region 113 and the center 111 of the cross-section of the fiber mount is greater than the distance between any point in the first region 112 and the center 111 of the cross-section of the fiber mount.
The light source can be a plane light source, and the light intensity of a light spot of the plane light source gradually decreases from the center to the edge. For example, the light source shown in fig. 3 is an approximate surface light source formed by an array LED, and the light emitted from a plurality of LEDs together form the light emitted from the array light source, so that the light emitted from the light source to the optical fiber bundle is more uniform.
The fiber mount may be circular in cross-section as shown in fig. 2, and the cross-section may be divided into a first region 112 and a second region 113. The first region may be an inner layer of the cross section, the second region may be an outer layer of the cross section, and a distance between any point in the outer layer and the center 111 of the cross section of the optical fiber mounting seat is greater than a distance between any point in the inner layer and the center 111 of the cross section of the optical fiber mounting seat.
The optical fiber mounting seat 110 is provided with a plurality of mounting holes 114, the mounting holes 114 are uniformly distributed in a first area and a second area, the mounting holes 114 are used for accessing optical fiber bundles, and each optical fiber bundle comprises at least one optical fiber; the distance between the end face of the optical fiber bundle accessed by the mounting hole of the first region and the light source 210 is a first distance, the distance between the end face of the optical fiber bundle accessed by the mounting hole of the second region and the light source 210 is a second distance, and the first distance is greater than the second distance.
The optical fiber bundle comprises at least one optical fiber, which can be a single optical fiber or an optical fiber bundle formed by combining a plurality of optical fibers. The mounting hole can be a straight hole with the diameter of the two ends being consistent, the optical fiber bundle can be directly accessed, and the diameter of the mounting hole is larger than that of the optical fiber bundle, so that the optical fiber bundle can pass through the mounting hole. The number of the mounting holes may be plural for realizing multi-hole detection or high-throughput detection by a large number of optical fibers, for example, the number of the holes may be 12, 24, 48, 96, 192, 384, etc.
As shown in fig. 4, the inner-layer installation hole 1124 is used to access the inner-layer optical fiber bundle 122, the outer-layer installation hole 1134 is used to access the outer-layer optical fiber bundle 121, the end surface of the inner-layer optical fiber bundle coincides with the edge of the optical fiber installation seat, the distance between the end surface of the inner-layer optical fiber bundle and the light source is a first distance, and the distance between the end surface of the outer-layer optical fiber bundle and the light source is a second distance.
In some embodiments, the fiber optic bundle extends a first length in the mounting hole of the first region, the fiber optic bundle extends a second length in the mounting hole of the second region, the first length is less than the second length, and the second length differs from the first length by a value in a range of 0.5-5 mm.
Referring to fig. 4, the outer fiber bundle may extend from the surface of the fiber mount by a distance (second length) to make the distance between the end surface and the light source shorter, where the first length is 0, the first length is smaller than the second length, and the difference between the second length and the first length is generally in the range of 0.5-5mm, so as to realize that the first distance is greater than the second distance.
Because the light intensity that the edge of light source sent is weaker than the light intensity that its center sent, therefore the optic fibre mount pad that this embodiment provided can make the distance of the terminal surface of outer lane optic fibre and light source closer than the distance of the terminal surface of inner circle optic fibre and light source, the decay is reduced before the light that outer lane department corresponds the light source gets into optic fibre, the decay is many before the light that inner circle department corresponds the light source gets into optic fibre to make all fiber bundles that the optic fibre mount pad inserts receive even illumination, can alleviate the problem that causes the detection accuracy rate to be low because of the illumination inequality, improved detection performance.
In some embodiments, the mounting hole is a stepped hole comprising a first step and a second step, the first step being disposed within the mounting hole of the first region and the second step being disposed within the mounting hole of the second region; the length difference range of the first step and the second step is 0.5-5mm, and the first step and the second step are used for enabling the first distance to be larger than the second distance.
Specifically, the stepped hole may have a structure with two ends having different diameters, wherein the diameter of the end of the stepped hole carrying the optical fiber bundle is larger than that of the optical fiber bundle, and the diameter of the end close to the light source is smaller than that of the optical fiber bundle, that is, a step having a smaller diameter than that of the optical fiber bundle is disposed in the mounting hole at the end close to the light source. When the optical fiber bundle enters the mounting hole from one end, the optical fiber bundle cannot penetrate out from the other end.
Referring to fig. 5, the outer-layer stepped hole 123 is used to access the outer-layer optical fiber bundle 121, and the inner-layer stepped hole 124 is used to access the inner-layer optical fiber bundle 122, since the specific example shown in fig. 5 only involves two layers of stepped holes, the outer-layer stepped hole is a common mounting hole, the end surface of the outer-layer optical fiber bundle is accessed to the edge of the optical fiber mounting seat, and the length of the second step is zero; the diameter of one end, close to the light source, of the inner-layer step hole is smaller than that of the optical fiber bundle, and the inner-layer step hole is used for enabling the end face of the inner-layer optical fiber bundle to keep the distance of the first step from the edge of the optical fiber mounting seat, so that the first distance between the end face of the inner-layer optical fiber bundle and the light source is larger than the second distance between the end face of the outer-layer optical fiber bundle and the light source.
In addition, the diameter of one end of the outer-layer step hole can be smaller than that of the optical fiber bundle, so that the end face of the outer-layer optical fiber bundle and the edge of the optical fiber installation seat keep a distance of a second step (the length of the second step is not zero), a larger distance (the length of the first step) needs to be kept between the end face of the inner-layer optical fiber bundle and the edge of the optical fiber installation seat, the difference range between the length of the first step and the length of the second step is 0.5-5mm, and the first distance is larger than the second distance.
In some embodiments, the cross-section of the fiber mount further includes a third region; the distance between any point in the third area and the center of the section of the optical fiber mounting seat is greater than the distance between any point in the second area and the center of the section of the optical fiber mounting seat; the distance between the end face of the optical fiber bundle accessed by the mounting hole of the third area and the light source is a third distance, and the second distance is larger than the third distance.
The cross-section of the fiber mount can also be divided into a first region, which can be an inner layer of the cross-section, a second region, which can be a middle layer of the cross-section, and a third region 115, which can be an outer layer of the cross-section, as shown in fig. 6. The distance between any point in the outer layer and the center of the cross section of the optical fiber installation seat is larger than the distance between any point in the middle layer and the center of the cross section of the optical fiber installation seat.
Further, inner mounting hole 1124 is used to access inner fiber bundle 122, middle mounting hole 1154 is used to access middle fiber bundle 152, and outer mounting hole 1134 is used to access outer fiber bundle 121. The distance between the end face of the inner layer optical fiber bundle and the light source is a first distance, the distance between the end face of the middle layer optical fiber bundle and the light source is a second distance, and the distance between the end face of the outer layer optical fiber bundle and the light source is a third distance.
In some embodiments, the fiber optic bundle extends a third length in the mounting hole of the third region; the first length is less than the second length, and the second length is less than the third length. In an alternative embodiment, the third length differs from the second length by a value in the range of 0.5-5mm, and the second length differs from the first length by a value in the range of 0.5-5 mm.
Specifically, the middle layer optical fiber bundle and the outer layer optical fiber bundle can extend out of the surface of the optical fiber mounting seat for a certain distance, so that the distance between the middle layer optical fiber bundle and the light source is shorter. The lengths of the portions of the middle layer optical fiber bundle and the outer layer optical fiber bundle protruding from the surface of the optical fiber mounting seat are respectively a second length and a third length, the first length shown in fig. 6 is 0, the first length is smaller than the second length, and the second length is smaller than the third length, so that the first distance is greater than the second distance, and the second distance is greater than the third distance.
In addition, the mounting hole can also be a step hole structure with the diameters of two ends not consistent, the distance between the optical fiber and the light source is designed to be in a gradient decreasing mode, and a gradient step hole is formed, so that a certain distance is kept between the optical fiber bundle and the edge of the optical fiber mounting seat. At this time, the following requirements are met: the inner layer optical fiber bundle keeps a first step distance from the edge of the optical fiber installation seat, the middle layer optical fiber bundle keeps a second step distance from the edge of the optical fiber installation seat, and the outer layer optical fiber bundle keeps a third step distance from the edge of the optical fiber installation seat.
Wherein, the length relation of three steps is: the length difference range of the first step is 0.5-5mm, and the first distance is larger than the second distance; the length difference range of the second step and the third step is 0.5-5mm, and the second distance is larger than the third distance.
In addition, in an alternative embodiment, the optical fiber mount may include two types of mounting holes, i.e., a straight hole with uniform diameters at both ends and a stepped hole with non-uniform diameters at both ends, for example, a first step is provided in the first region (inner layer), and straight holes are provided in the second region (middle layer) and the third region (outer layer). The end face of the optical fiber bundle is superposed with the edge of the straight hole in the second area, and the optical fiber bundle extends out of the straight hole in the third area by a certain length; the length of the first step is 0.5-5mm, and a first distance between the end face of the inner-layer optical fiber bundle and the light source is larger than a second distance between the end face of the middle-layer optical fiber bundle and the light source; the length of the optical fiber bundle extending out of the straight hole in the third area is 0.5-5mm, and the second distance between the end face of the middle optical fiber bundle and the light source is larger than the third distance between the end face of the outer optical fiber bundle and the light source. Or a first step and a second step are provided in the first region (inner layer) and the second region (middle layer), respectively, and the optical fiber bundle is caused to protrude from the mounting hole by a certain length in the third region. The first distance is greater than the second distance only by using the stepped hole or extending from the mounting hole, and the second distance is greater than the third distance, which is not limited to this. In the embodiments, the mounting holes of the inner layer or the middle layer are stepped holes, the mounting holes of the outer side are arranged in a mode that the optical fiber bundles extend out, and the outer mounting holes of the optical fiber mounting seat are arranged on the outer side, so that the distance of the optical fiber bundles extending out is easily arranged, the distance of the optical fiber bundles extending out can be conveniently adjusted, and the effect of more uniform light beams is achieved.
In a possible embodiment, the difference between the first distance and the second distance is in the range of 0.5-5mm and the difference between the second distance and the third distance is in the range of 0.5-5 mm.
The difference between the distances may be adjusted according to the light source, for example, when the light source has a large difference in illumination intensity for each layer of optical fiber of the optical fiber installation base, the difference between the first distance and the second distance may be set to be 3mm, and the difference between the second distance and the third distance may be set to be 1 mm; when the difference of the illumination intensity of each layer of optical fiber of the optical fiber installation base by the light source is small, the difference between the first distance and the second distance can be set to be 3mm, and the difference between the second distance and the third distance can be set to be 2 mm. Or the dynamic adjustment can be carried out according to the current illumination so as to achieve the aim that the optical fibers in different areas can receive more uniform illumination.
When the difference between the distances between the optical fiber bundle accessed in the adjacent different areas and the light source is not between 0.5 and 5mm, for example, the difference between the first distance and the second distance is less than 0.5mm or greater than 5mm, the attenuation performance of light is generally poor, so that the optical fiber bundle in the different areas is not uniformly illuminated, thereby affecting the detection accuracy.
In some embodiments, as shown in FIG. 7, the side of the fiber mount is provided with a plurality of screws 130, one for each mounting hole 114.
Referring specifically to fig. 7, the optical fiber bundle is mounted on the optical fiber mounting base by fixing the optical fiber bundle with screws 130 on the side of the mounting base, each hole is correspondingly provided with one screw, and the screws of the inner layer holes penetrate through the gaps between the outer layer holes.
Compared with the fixing mode that bonding and the like can be installed once in the prior art, the screw fixing method has the advantages that the installation position of the optical fiber bundle can be conveniently adjusted, when the optical fiber is installed inaccurately, or the height difference of the installed optical fiber is not in accordance with the range of 0.5-5mm, so that the light intensity among different optical fibers is uneven, the light intensity distribution of different optical fibers can be more uniform through the screw disassembling and reassembling modes and the like. In addition, the use of screw cooperation adjusting screw can fix the cable of different diameter specifications and compress tightly, improve equipment practicality.
In a possible embodiment, the end of the screw contacting the fiber bundle is an arc-shaped surface, and the arc-shaped surface is used for increasing the contact area with the fiber bundle. Because the optical fiber has the cylindrical surface, the optical fiber bundle is formed by combining a plurality of optical fibers, when the end part of the screw is in an arc surface, the contact area with the optical fiber or the optical fiber bundle can be increased, so that the fixation is firmer, and the damage to the optical fiber caused by too small contact area can be prevented.
In some embodiments, referring to fig. 8, the side of the fiber mount bearing the end face of the fiber bundle is provided with a protrusion 140 for fixedly connecting with the filtering unit. Specifically, the part of the optical fiber installation seat bearing the end face of the optical fiber bundle is in a convex shape, and the convex part can be installed on the filter wheel, so that the distance between the optical filter on the filter wheel and the optical fiber is closer and the optical filter and the optical fiber are coaxially installed, and the loss of light is reduced.
The embodiment of the invention provides an optical fiber mounting seat, which can adjust the distance between the end face of each optical fiber bundle at different positions and a light source; the optical fiber bundle is fixed by using the screws on the side surface of the optical fiber mounting seat, and when the position of the optical fiber bundle is installed wrongly or the distance difference of the installed optical fibers does not accord with the range, the light intensity among different optical fibers is uneven, the adjustment is convenient; the light source adopts an LED array surface light source, so that the light is emitted more uniformly; the part of the optical fiber mounting seat bearing the optical fiber bundle is convex, and the convex part is finally mounted on the filter wheel, so that the distance between the optical filter on the filter wheel and the optical fiber is closer and coaxially mounted, and the effect of reducing light loss is realized.
By the design, when multi-hole detection is carried out and high-flux detection is carried out by using optical fibers with large quantity, light emitted by a light source is more uniform, light received by the optical fibers with different positions on the end face of the optical fiber converged by the light source end is more uniform, and the uniformity of light finally irradiated into each sample hole is good; the uniform light distribution can obtain uniform sample yield, and can also ensure that the detection results among different sample holes do not have obvious difference, ensure the accuracy of the detection results and improve the detection performance.
The embodiment of the invention also provides a PCR optical module, which comprises: the light source and the optical fiber installation seat provided by any one of the above embodiments, wherein the center of the optical fiber installation seat and the center of the light source are on the same horizontal line.
In some embodiments, the light module further comprises: a lens and filter unit having a structure as shown in fig. 1, wherein the lens 310 is disposed between the light source 210 and the optical fiber mount 110, and the filter unit 410 is disposed between the lens 310 and the optical fiber mount 110; the center of the lens 310, the center of the filter unit 410, and the center of the fiber mount 110 are all on the same horizontal line.
Specifically, as shown in fig. 9, the PCR optical module includes: light source, lens, filter wheel 502, fiber mount, and optical fiber. In addition, the PCR optical module may further include a motor 501, a lens mount 503, a connector 504, a heat sink 505, a fan 506, and an optical filter. The light source is arranged on the surface of the radiator and used for radiating heat for the light source; the lens installation part is used for installing the lens, light emitted by the light source irradiates a filter wheel which can be driven by a motor to rotate after being collimated by the lens in the lens installation part, and the filter wheel is provided with a plurality of optical filters which can be used for selectively filtering out light with required wavelength.
The embodiment of the invention also provides a PCR instrument which comprises the PCR optical module provided by any one of the embodiments.
The PCR optical module and the PCR instrument provided by the embodiment of the invention have the same technical characteristics as the optical fiber mounting seat provided by the embodiment, so the same technical problems can be solved, and the same technical effects can be achieved.
It should be noted that: like reference numbers and letters indicate like items in the figures, and thus once an item is defined in a figure, it need not be further defined or explained in subsequent figures, and moreover, the terms "first," "second," "third," etc. are used merely to distinguish one description from another and are not to be construed as indicating or implying relative importance.
Finally, it should be noted that: the above-mentioned embodiments are only specific embodiments of the present invention, which are used for illustrating the technical solutions of the present invention and not for limiting the same, and the protection scope of the present invention is not limited thereto, although the present invention is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: any person skilled in the art can modify or easily conceive the technical solutions described in the foregoing embodiments or equivalent substitutes for some technical features within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit and scope of the embodiments of the present invention, and they should be construed as being included therein.

Claims (10)

1. An optical fiber mount, wherein the center of the optical fiber mount is on the same horizontal line as the center of a light source, the light source being configured to provide light to an optical fiber bundle;
the cross section of the optical fiber installation seat comprises a first area and a second area, and the distance between any point in the second area and the center of the cross section of the optical fiber installation seat is greater than the distance between any point in the first area and the center of the cross section of the optical fiber installation seat;
the optical fiber mounting seat is provided with a plurality of mounting holes, the mounting holes are uniformly distributed in the first area and the second area, the mounting holes are used for accessing the optical fiber bundles, and each optical fiber bundle comprises at least one optical fiber;
the distance between the end face of the optical fiber bundle accessed by the mounting hole of the first region and the light source is a first distance, the distance between the end face of the optical fiber bundle accessed by the mounting hole of the second region and the light source is a second distance, and the first distance is greater than the second distance.
2. The fiber mount of claim 1, wherein the cross-section of the fiber mount further comprises a third region; the distance between any point in the third region and the center of the cross section of the optical fiber installation seat is greater than the distance between any point in the second region and the center of the cross section of the optical fiber installation seat, the distance between the end face of the optical fiber bundle accessed into the installation hole of the third region and the light source is a third distance, and the second distance is greater than the third distance.
3. The fiber mount of claim 2, wherein the mounting hole is a stepped hole comprising a first step and a second step; the first step is arranged in the mounting hole of the first area, and the second step is arranged in the mounting hole of the second area;
the length difference range of the first step and the second step is 0.5-5mm, and the first step and the second step are used for enabling the first distance to be larger than the second distance.
4. The fiber mount of claim 3, wherein the stepped bore further comprises a third step disposed within the mounting bore of the third region;
the length difference range of the second step and the third step is 0.5-5mm, and the third step is used for enabling the second distance to be larger than the third distance.
5. The fiber mount of claim 2, wherein the fiber bundle extends a first length in the mounting hole of the first region, the fiber bundle extends a second length in the mounting hole of the second region, and the fiber bundle extends a third length in the mounting hole of the third region; the first length is less than the second length, and the second length is less than the third length.
6. The fiber mount of claim 5, wherein the third length differs from the second length by a value in a range of 0.5-5mm, and the second length differs from the first length by a value in a range of 0.5-5 mm.
7. The fiber mount of claim 1, wherein a plurality of screws are disposed on a side of the fiber mount, each screw corresponding to each mounting hole, and an end of each screw contacting the fiber bundle is curved.
8. The optical fiber mount of claim 1, wherein a side of the optical fiber mount carrying the end face of the optical fiber bundle is provided with a protrusion for fixedly connecting with a filtering unit.
9. A PCR optical module, comprising: a light source and the fiber mount of any one of claims 1-8.
10. A PCR instrument comprising the PCR optical module according to claim 9.
CN202010283329.9A 2020-04-10 2020-04-10 Optical fiber mounting seat, PCR optical module and PCR instrument Active CN111707613B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202010283329.9A CN111707613B (en) 2020-04-10 2020-04-10 Optical fiber mounting seat, PCR optical module and PCR instrument
PCT/CN2020/092542 WO2021203538A1 (en) 2020-04-10 2020-05-27 Optical fiber mounting base, pcr optical module, and pcr amplifier

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010283329.9A CN111707613B (en) 2020-04-10 2020-04-10 Optical fiber mounting seat, PCR optical module and PCR instrument

Publications (2)

Publication Number Publication Date
CN111707613A CN111707613A (en) 2020-09-25
CN111707613B true CN111707613B (en) 2021-02-02

Family

ID=72536396

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010283329.9A Active CN111707613B (en) 2020-04-10 2020-04-10 Optical fiber mounting seat, PCR optical module and PCR instrument

Country Status (2)

Country Link
CN (1) CN111707613B (en)
WO (1) WO2021203538A1 (en)

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5898081A (en) * 1981-12-03 1983-06-10 Takashi Mori Photosynthetic apparatus
JPS6080713A (en) * 1983-10-07 1985-05-08 Sumitomo Electric Ind Ltd Optical displacement sensor
CA2129787A1 (en) * 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
JPH10253841A (en) * 1997-03-07 1998-09-25 Rikagaku Kenkyusho Image forming optical device
US6982431B2 (en) * 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6453104B1 (en) * 1999-12-28 2002-09-17 Mitsubishi Rayon Co., Ltd. Optical fiber cable and optical fiber cable with plug
US6465775B2 (en) * 2000-12-19 2002-10-15 Schlumberger Technology Corporation Method of detecting carbon dioxide in a downhole environment
US6878254B2 (en) * 2001-03-02 2005-04-12 Iowa State University Research Foundation, Inc. Size separation of analytes using monomeric surfactants
JP2002365488A (en) * 2001-06-04 2002-12-18 Kyoto Denkiki Kk Light source device
US7486861B2 (en) * 2003-01-15 2009-02-03 Negevtech Ltd. Fiber optical illumination system
DE102006036171B4 (en) * 2006-07-28 2008-10-09 Analytik Jena Ag Arrangement and method for multichannel fluorescence measurement in PCR samples
GB201005704D0 (en) * 2010-04-06 2010-05-19 It Is Internat Ltd Improvements in systems for chemical and/or biochemical reactions
US20110312102A1 (en) * 2010-06-16 2011-12-22 Samsung Techwin Co., Ltd. Light transmissive temperature control apparatus and bio-diagnosis apparatus including the same
CN102759797A (en) * 2012-07-06 2012-10-31 胡宵 Writing device
CN104568875B (en) * 2014-12-22 2017-02-22 北京工业大学 Rotary scanning real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection system
CN104614351A (en) * 2015-01-21 2015-05-13 南京中科神光科技有限公司 Rapid and multi-channel real-time fluorescence quantification detection device
JP6813953B2 (en) * 2016-02-29 2021-01-13 シスメックス株式会社 Blood coagulation analyzer and blood coagulation analysis method
CN106047687B (en) * 2016-04-08 2018-05-18 芜湖达辉生物科技有限公司 Ribonucleic acid chain type polymerize amplified reaction detection device and its method for carrying out DNA concentration detection
CN208351038U (en) * 2018-05-03 2019-01-08 苏州奥荣光电有限公司 A kind of optical splitter uniform more than one point
CN209783771U (en) * 2019-04-15 2019-12-13 广州大学 Optical fiber sensor and manufacturing mold
CN110161642B (en) * 2019-06-03 2023-08-04 四川九洲线缆有限责任公司 Assembly fixture for concentric arrangement of inner and outer optical fiber bundles and use method
CN110218647B (en) * 2019-06-27 2020-05-12 杭州安塔生物科技有限公司 PCR reaction device
CN110724733B (en) * 2019-10-30 2022-11-11 东南大学 Sequencing chip for high-resolution space transcriptome

Also Published As

Publication number Publication date
CN111707613A (en) 2020-09-25
WO2021203538A1 (en) 2021-10-14

Similar Documents

Publication Publication Date Title
US5436805A (en) Thermally insulated distributed light network from a central light source
US8268249B2 (en) Analytical device with lightguide illumination of capillary and microgroove arrays
US6904225B2 (en) Optical waveguide plate of surface light emitting apparatus
EP1925877A1 (en) Illumination device
US7405824B2 (en) Optical coupling system of light measuring device and sample
KR20110055539A (en) Fluorescent tube replacement having longitudinally oriented leds
JP2012164651A (en) Flat panel type light source for transmission lighting device for microscope
CN111707613B (en) Optical fiber mounting seat, PCR optical module and PCR instrument
US6469833B2 (en) Illumination optical system and an illumination lens element
US20130294064A1 (en) Dimpled light distribution plates
JP2005301016A (en) Light guide plate for backlight of liquid crystal display
US7054004B2 (en) Capillary array and capillary array photodetector
JP2016201360A (en) Luminaire
US9063312B2 (en) Optical-fiber-based light source
JP2012515897A (en) Spatially controlled illumination of a biological sample array through a wedge-shaped support
KR102085955B1 (en) A lighting device using a led
US11150513B2 (en) Reflective diffusing lens and light emitting module comprising the same
CN211452649U (en) Optical fiber temperature measurement module of multichannel unification
CN212255894U (en) Light splitter
CN219348762U (en) Optical chamber structure for liquid chromatograph detector
CN217559730U (en) Light-emitting device and lighting device
CN217878007U (en) Novel multi-wavelength photometer
CN210532167U (en) Be applied to ultraviolet curing's lamp body component
CN214069083U (en) Linear ultra-fine line light spot semiconductor laser output system
KR101970690B1 (en) Optical receiver for measuring multiple light signals

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB02 Change of applicant information
CB02 Change of applicant information

Address after: Hangzhou City, Zhejiang province 310000 high tech Industrial Development Zone Waterfront Road 1192

Applicant after: Hangzhou bori Technology Co., Ltd

Address before: 310000 no.1192 Bin'an Road, Binjiang District, Hangzhou City, Zhejiang Province

Applicant before: HANGZHOU BIOER TECHNOLOGY Co.,Ltd.

GR01 Patent grant
GR01 Patent grant