CN108379335A - The weight-reducing purposes of beans taro leaf ethanol extract - Google Patents
The weight-reducing purposes of beans taro leaf ethanol extract Download PDFInfo
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- CN108379335A CN108379335A CN201810225828.5A CN201810225828A CN108379335A CN 108379335 A CN108379335 A CN 108379335A CN 201810225828 A CN201810225828 A CN 201810225828A CN 108379335 A CN108379335 A CN 108379335A
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- ethanol extract
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- 235000010627 Phaseolus vulgaris Nutrition 0.000 title claims abstract description 81
- 239000000469 ethanolic extract Substances 0.000 title claims abstract description 69
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
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Abstract
The invention discloses a kind of weight-reducing purposes of beans taro leaf ethanol extract, and developing natural diet products for fields such as medicine, Food Sciences provides new resources.Beans taro leaf ethanol extract in the present invention is proved through high fat diet obesity-induced mice experimental evaluation, can significantly reduce obesity mice weight, reduces triglycerides and total cholesterol level in serum and liver, and function and effect are comprehensively and notable.Therefore, beans taro leaf ethanol extract of the invention is applied to prepare diet food or drug, is occurred with treating and preventing fat and its chronic diseases.
Description
Technical field
The present invention relates to field of medicaments, and in particular to a kind of weight-reducing purposes of beans taro leaf ethanol extract.
Background technology
Beans taro (ApiosamericanaMedikus) originates in the Canadian to Fla. of eastern North America
Southern areas belong to perennial legume (Papilionaceae subfamily).Its edible portion is underground stem tuber, and its protein content
Higher than other plant stem tuber, it is a kind of not only nutrition but also has the crop of economic value, thus often by American Indian as staple food.Beans taro
The domestic successful introduction from 2009, and by gradually in the ground such as Fuyang, Jinhua, Long You, Wenzhou popularizing planting.Research shows that beans taro is not
Same position (flower, rattan, leaf, stem tuber) contain abundant free amino acid, soluble protein, polysaccharide, saponin(e, flavones, isoflavones,
Vitamin C, vitamin E and mineral matter and other components.Wherein, the content of vitamin E highest of beans taro leaf, while also containing high level
Polysaccharide, total saposins and isoflavones have great potentiality to be exploited.
With the rapid development of modern social economy, the living standard of the people significantly improves, obesity becomes the whole world and generally flows
Capable health problem.It is reported in March, 2011 according to the World Health Organization and shows that the whole world in 2008 has more than 1,500,000,000 adults and is in
Overweight level, wherein at least 500,000,000 be fat.The year two thousand thirty is expected, there will be problem of obesity for the population in the U.S. 42%.U.S. will be exceeded
The present population of state 30,000,000.Medical treatment cost increase related to this will reach nearly 550,000,000,000 dollars.And it was delivered at home according to 2010
Research report claim, for population of being obese up to 3.25 hundred million people, amplification is more than the U.S., Britain and Australia at present for China.7 years old or less youngsters
Tong Zhongyou nearly 1/5th is overweight, and 7% is Obese children, ratio Yuan Chao European countries.This number will be also possible to for 20 years in future
It doubles.The common complication of disorders of lipid metabolism has fatty liver, hypertension, diabetes, coronary heart disease, cerebral apoplexy etc..Thus
As it can be seen that disorders of lipid metabolism has become the universal health problem in the whole world, prevention and treatment disorders of lipid metabolism has become 21 generation
The important health problem of discipline, how the generation of prevention and treatment disorders of lipid metabolism and its related complication has caused the whole world
The concern of person.Therefore, the researcher of many nutrition and health care food pays special attention in relation to fat and chronic disease hair at present
The raw and research prevented and topic, but the anti-obesity activity of beans taro leaf ethanol extract is rarely reported.
Invention content
It is the necks such as medicine, Food Science present invention aims at a kind of weight-reducing purposes of beans taro leaf ethanol extract is provided
Domain develops natural diet products and provides new resources.
In order to solve the above technical problems, the present invention provides a kind of weight-reducing purposes of beans taro leaf ethanol extract：
It is used to prepare diet food or drug；
The improvement of weight-reducing purposes as beans taro leaf ethanol extract of the present invention：
The diet food or drug are the food or drug for reducing high fat diet and leading obesogenous weight.
Weight-reducing purposes as beans taro leaf ethanol extract of the present invention is further improved：
The diet food or drug are the food or drug for reducing adipose tissue mass.
Weight-reducing purposes as beans taro leaf ethanol extract of the present invention is further improved：
The diet food or drug be reduce Triglycerides in Serum (TG) and total cholesterol (TC) content food or
Drug.
Weight-reducing purposes as beans taro leaf ethanol extract of the present invention is further improved：
The diet food or drug be reduce in liver the food of triglycerides (TG) and total cholesterol (TC) content or
Drug.
Weight-reducing purposes as beans taro leaf ethanol extract of the present invention is further improved：
The diet food or drug are to reduce glutamic-pyruvic transaminase (ALT) and glutamic-oxalacetic transaminease (AST) content in serum
Food or drug.
Weight-reducing purposes as beans taro leaf ethanol extract of the present invention is further improved：
The diet food or drug are to adjust glutamic-pyruvic transaminase (ALT) and glutamic-oxalacetic transaminease (AST) content in liver
Food or drug.
Weight-reducing purposes as beans taro leaf ethanol extract of the present invention is further improved：
The preparation method of the beans taro leaf ethanol extract includes the following steps：
1., according to 1g:5~10ml (preferably 1g:Solid-liquid ratio 8ml) is by the 85% of beans taro leaf and the hydrochloric acid containing 0.1mol/L
It is beaten after ethyl alcohol mixing, gained slurries carry after 45 ± 5 DEG C of ultrasounds (40,000Hz ultrasonic frequencies) extract 240 ± 10min
Liquid centrifugation (4000 turns/min centrifuges 30min) is taken, supernatant is obtained；
2., 1. filter residue that step centrifuges to gained replace beans taro leaf 1. to repeat extraction twice by step, merge filtrate three times,
Concentrated by rotary evaporation obtains concentrate until for the 5~10% of original volume；
3., by step 2. gained concentrate isolated and purified using macroreticular resin；It is water-soluble with 1% formic acid of volumetric concentration
For liquid as eluent, the methanol aqueous solution to contain 0.05% formic acid collects eluent as eluant, eluent；
4., by step 3. gained eluent rotary evaporation be condensed into the 5~10% of most original volume, obtain pulpous state concentrate；
5., by step 4. gained pulpous state concentrate in -70~-90 DEG C of 5~7h of pre-freeze, then use vacuum freeze drier
(technological parameter that vacuum freeze drier is set as -40 DEG C, 1.2Pa) is dried (moisture content≤0.1%) at powdered, obtains
To beans taro leaf ethanol extract.
Weight-reducing purposes as beans taro leaf ethanol extract of the present invention is further improved：
The step 3. the specific steps are：
Macroreticular resin is activated with the methanol of twice of column volume first, the distilled water of three times column volume is balanced；
Then with the flow velocity loading of 0.4~0.6mL/min (preferably 0.5mL/min), after liquid to be extracted is fully adsorbed,
1% aqueous formic acid of volumetric concentration used as eluent eluted that (purpose of elution is to remove protein, sugar, acid etc.
Substance), the dosage of the eluent is twice of column volume, and flow velocity is 0.4~0.6mL/min (preferably 0.5mL/min)；Finally
The methanol aqueous solution for containing 0.05% formic acid is used to be eluted as eluant, eluent, the dosage three times column volume of the eluant, eluent, stream
Speed is 0.4~0.6mL/min (preferably 0.5mL/min)；Collect eluent.
Note：The preparation method of methanol aqueous solution containing 0.05% formic acid is：It is water-soluble in 90% methanol of 100ml volumetric concentrations
The formic acid of 0.05ml is added in liquid.The technological parameter that above-mentioned rotary evaporation is set is 38 DEG C of temperature, vacuum degree -0.09MPa.It is adopted
Macroreticular resin is macroreticular resin AB-8 (Tianjin Xing Nanyun energy Polymer Technologies Co., Ltd).
The invention discloses a kind of preparation method of beans taro leaf ethanol extract and its weight-reducing purposes, are medicine, food section
Etc. fields develop natural diet products and provide new resources.Beans taro leaf ethanol extract in the present invention induces fertilizer through high fat diet
Fat mouse experiment evaluation proves, can significantly reduce obesity mice weight, and it is solid to reduce triglycerides and total courage in serum and liver
Alcohol content, function and effect are comprehensively and notable.Therefore, beans taro leaf ethanol extract of the invention is applied to prepare diet food or medicine
Product are occurred with treating and preventing fat and its chronic diseases.
The usage of the beans taro leaf ethanol extract of the present invention is oral, dosage about 150~250mg every time, three times a day.
The present invention compared with the existing technology, has the following advantages：
1. present invention firstly discovers that beans taro leaf ethanol extract can alleviate the increasing of high fat diet obesity-induced mice weight
Add, reduce triglycerides and total cholesterol level in obesity mice serum and liver, show significant Weight-reducing and lipid-lowering effect, has
There is good development prospect.
2. the present invention provides new medical application for beans taro leaf ethanol extract, a new application field has been expanded.
Description of the drawings
Fig. 1 is influence of the various concentration beans taro leaf ethanol extract to mouse weight in the present invention.
Fig. 2 is various concentration beans taro leaf ethanol extract in the present invention to glutamic-pyruvic transaminase in mouse liver (ALT) content
Influence.
Fig. 3 is various concentration beans taro leaf ethanol extract in the present invention to glutamic-oxalacetic transaminease in mouse liver (AST) content
Influence.
Fig. 4 is various concentration beans taro leaf ethanol extract in the present invention to the shadow of triglycerides in mouse liver (TG) content
It rings.
Fig. 5 is various concentration beans taro leaf ethanol extract in the present invention to the shadow of total cholesterol in mouse liver (TC) content
It rings.
Note：Fig. 1~Fig. 5 of the present invention, in 1~table of table 2：
LFD represents low fat control group (low fat feed of the feeding containing 10% fat)；
HFD represents control group high in fat (high lipid food of the feeding containing 45% fat)；
HFD+L represents low dose group (high lipid food+gavage 100mg/ (kgd) beans taro leaf second of the feeding containing 45% fat
Alcohol extracting thing)；
HFD+H represents high dose group (high lipid food+gavage 300mg/ (kgd) beans taro leaf second of the feeding containing 45% fat
Alcohol extracting thing).
Specific implementation mode
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
The weight-reducing purposes of embodiment 1, beans taro leaf ethanol extract, as Figure 1-Figure 5, beans taro leaf ethanol extract have
Significant Weight-reducing and lipid-lowering effect.
The specific preparation process of beans taro leaf ethanol extract is as follows：
(1), according to 1g：After the solid-liquid ratio of 8ml mixes 85% ethyl alcohol of beans taro leaf and the hydrochloric acid containing 0.1mol/L, through squeezing the juice
Machine processing mashing.Gained slurries are under the conditions of 45 DEG C, after ultrasonic (40,000Hz ultrasonic frequencies) extraction 240min, extracting solution
4000 leave heart 30min, and extracting solution centrifugation obtains supernatant.
(2), the filter residue of step (1) centrifugation gained is replaced into beans taro leaf in step (1), repeats extraction 2 times, merging is filtered three times
Liquid, by the supernatant of gained, in Rotary Evaporators, (technological parameter that rotary evaporation is set is 38 DEG C of temperature, vacuum degree-
Rotary evaporation concentration 0.09MPa) is carried out until for the 10% of original volume.
(3), revolving gained concentrate is isolated and purified using macroreticular resin, used macroreticular resin is macropore tree
Fat AB-8 (Tianjin Xing Nanyun energy Polymer Technologies Co., Ltd), follows the steps below successively：
Then with the flow velocity loading of 0.5mL/min, applied sample amount is 200ml (for the 10% of column volume)；Extracting solution is fully inhaled
Attached, 1% formic acid (that is, 1% aqueous formic acid of volumetric concentration) elution removes the substances such as protein, sugar, acid, the use of the eluent
Amount is twice of column volume, flow velocity 0.5mL/min；Finally use the methanol aqueous solution for containing 0.05% formic acid abundant as eluant, eluent
Elution, the dosage three times column volume of the eluant, eluent, flow velocity 0.5mL/min；Collect eluent.Methanol containing 0.05% formic acid
The preparation method of aqueous solution is：The formic acid of 0.05ml is added in 90% methanol aqueous solution of 100ml volumetric concentrations；
(4), step (3) macroreticular resin is isolated and purified into gained eluent rotary evaporation (the technique ginseng of rotary evaporation setting
Number is 38 DEG C of temperature, vacuum degree -0.09MPa) until for the 10% of original volume, obtain pulpous state concentrate；
(5), then the pulpous state concentrate obtained by step (4) is dried at -80 DEG C of pre-freeze 6h with vacuum freeze drier
48h (technological parameter that vacuum freeze drier is set as -40 DEG C, 1.2Pa) be dried into it is powdered (moisture content≤
0.1%) beans taro leaf ethanol extract, is obtained.
The beans taro leaf ethanol extract prepared in aforementioned manners is intervened the C57BL/6 fertilizer induced by high fat diet
Fat mouse observes its changes of weight and detects triglyceride and cholesterol level variation in serum and liver, the specific steps are：
1, experimental animal grouping and processing；
50 male C57BL/6 mouse are put into animal house and feed, and feeding environment is 12h alternation of light and darkness, and temperature is 23 ± 3
DEG C, relative humidity 50%.After adaptable fed 1 week, mouse is randomly divided into 5 groups, every group 10.
Group designs：Low fat control group (LFD), low fat feed of the feeding containing 10% fat；Control group high in fat
(HFD), high lipid food of the feeding containing 45% fat；Low dosage beans taro leaf ethanol extract group (HFD+L), feeding is containing 45% fat
High lipid food and gavage 100mg/ (kgd) beans taro leaf ethanol extract；High dose beans taro leaf ethanol extract group HFD+H),
High lipid food and gavage 300mg/ (kgd) beans taro leaf ethanol extract of the feeding containing 45% fat.Mouse is free during experiment
Feed and drinking-water, Therapy lasted 12 weeks.
2, influence of the record beans taro leaf ethanol extract to obesity mice weight：
Weight and food ration are recorded during experiment weekly.According to Fig. 1 it can be found that beans taro leaf ethanol extract is intervened 12 weeks
Afterwards, compared with control group high in fat, low dose group and high dose group can alleviate the C57BL/6 mouse weights that high fat diet induces and increase
Add.
The influence of embodiment 2, beans taro leaf ethanol extract to organ indexs such as obesity mice fat, livers；
For 24 hours by the mouse fasting after gavage in embodiment 1 12 weeks, it weighs in, eye socket takes mouse cervical dislocation after blood
It puts to death, takes kidney week and epididymis peripheral adipose, quickly win the heart, liver,spleen,kidney, brown fat etc. and organize and record weight, specific number
According to as shown in table 1.
Influence of the 1 beans taro leaf ethanol extract intervention of table to mice organs index
According to table 1 it is found that the beans taro leaf ethanol extract of various dose can significantly reduce testis week in obesity mice body
Fat and perirenal fat index, compared with control group high in fat the leaf ethanol extract intervention of beans taro to liver, brown fat, heart,
Kidney, spleen and pancreas index do not make significant difference.The above result shows that beans taro leaf ethanol extract can significantly improve it is high in fat
The variation of Diet-Induced Obesity mouse body fat index.
Embodiment 3, beans taro leaf ethanol extract to obesity mice serum triglyceride, total cholesterol, glutamic-pyruvic transaminase and
The influence of glutamic-oxalacetic transaminease content；
For 24 hours by the mouse fasting after gavage in embodiment 1 12 weeks, eye socket puts to death mouse cervical dislocation after taking blood, blood
Sample 1500 leaves heart 15min, takes serum keeping and using Toshiba's automatic clinical chemistry analyzer to Triglycerides in Serum and always
Cholesterol level is measured, and specific data are as shown in table 2.
Influence of the 2 beans taro leaf ethanol extract intervention of table to mice serum index
According to table 2 it is found that high dose group and low dose group can significantly reduce obesity mice Triglycerides in Serum, courage
The content of sterol content, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease.The above result shows that beans taro leaf ethanol extract can improve it is high in fat
The variation of triglycerides, total cholesterol, glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease content in Diet-Induced Obesity mice serum.
Embodiment 4, beans taro leaf ethanol extract are to the shadow of obesity mice liver glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease content
It rings；
For 24 hours by the mouse fasting after gavage in embodiment 1 12 weeks, it weighs in, eye socket takes mouse cervical dislocation after blood
It puts to death, mouse liver is taken to turn ammonia according to glutamic-pyruvic transaminase assay kit (Bioengineering Research Institute is built up in Nanjing) and millet straw
Enzyme assay kit (Bioengineering Research Institute is built up in Nanjing) specification is measured.
According to fig. 2 with Fig. 3 it is found that glutamic-pyruvic transaminase and millet straw turn ammonia in high in fat group of mouse liver compared with low fat control group
The content of enzyme dramatically increases, and can significantly improve after the beans taro leaf ethanol extract intervention of low dosage and be made by high fat diet
At liver in glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease increase, but can be shown after the beans taro leaf ethanol extract intervention of high dose
Writing reduces the accumulation of glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease in liver.The above result shows that the beans taro leaf ethanol extract of high dose
With notable protect liver effect.
The influence of embodiment 5, beans taro leaf ethanol extract to obesity mice liver tg and total cholesterol level；
For 24 hours by the mouse fasting after gavage in embodiment 1 12 weeks, it weighs in, eye socket takes mouse cervical dislocation after blood
It puts to death, mouse liver is taken to contain according to triglyceride assay kit (Bioengineering Research Institute is built up in Nanjing) and total cholesterol
Amount assay kit (Bioengineering Research Institute is built up in Nanjing) specification is measured.
According to Fig. 4 with Fig. 5 it is found that triglycerides and total cholesterol contain in high in fat group of mouse liver compared with low fat control group
Amount dramatically increases, and can significantly improve the liver as caused by high fat diet after the beans taro leaf ethanol extract intervention of various dose
The increase of dirty middle total cholesterol and triglycerides.The above result shows that beans taro leaf ethanol extract can effectively be alleviated in liver
The accumulation of triglycerides and total cholesterol.
To sum up the present invention proposes a kind of preparation method and its weight-reducing purposes of beans taro leaf ethanol extract, is medicine, food
The fields such as science develop natural diet products and provide new resources.It is related to beans taro leaf ethanol extract in preparing diet products simultaneously
Application.
Listed above is only several examples of the implementation of the present invention.It is clear that the invention is not restricted to which above example, may be used also
With there are many deformations.All changes that those skilled in the art directly can export or associate from present disclosure
Shape is considered as protection scope of the present invention.
Claims (9)
1. the weight-reducing purposes of beans taro leaf ethanol extract, it is characterised in that：It is used to prepare diet food or drug.
2. the weight-reducing purposes of beans taro leaf ethanol extract according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing high fat diet and leading obesogenous weight.
3. the weight-reducing purposes of beans taro leaf ethanol extract according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing adipose tissue mass.
4. the weight-reducing purposes of beans taro leaf ethanol extract according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing Triglycerides in Serum and total cholesterol level.
5. the weight-reducing purposes of beans taro leaf ethanol extract according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing triglycerides and total cholesterol level in liver.
6. the weight-reducing purposes of beans taro leaf ethanol extract according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease content in serum.
7. the weight-reducing purposes of beans taro leaf ethanol extract according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for adjusting glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease content in liver.
8. according to the weight-reducing purposes of any beans taro leaf ethanol extracts of claim 1-7, it is characterised in that：
The preparation method of the beans taro leaf ethanol extract includes the following steps：
1., according to 1g:The solid-liquid ratio of 5~10ml is beaten after mixing 85% ethyl alcohol of beans taro leaf and the hydrochloric acid containing 0.1mol/L, institute
Slurries are obtained after 45 ± 5 DEG C of 240 ± 10min of ultrasound extraction, extracting solution centrifugation obtains supernatant；
2., 1. filter residue that step centrifuges to gained replace beans taro leaf 1. to repeat extraction twice by step, merge filtrate three times, revolving
Concentration obtains concentrate until for the 5~10% of original volume；
3., by step 2. gained concentrate isolated and purified using macroreticular resin；Made with 1% aqueous formic acid of volumetric concentration
For eluent, the methanol aqueous solution to contain 0.05% formic acid collects eluent as eluant, eluent；
4., by step 3. gained eluent rotary evaporation be condensed into the 5~10% of most original volume, obtain pulpous state concentrate；
5., by step 4. gained pulpous state concentrate in -70~-90 DEG C of 5~7h of pre-freeze, then done with vacuum freeze drier
It is dry at powdered, obtain beans taro leaf ethanol extract.
9. the weight-reducing purposes of beans taro leaf ethanol extract according to claim 8, it is characterised in that：
The step 3. the specific steps are：First macroreticular resin, pair of three times column volume are activated with the methanol of twice of column volume
Water is steamed to be balanced；Then volumetric concentration is used after liquid to be extracted is fully adsorbed with the flow velocity loading of 0.4~0.6mL/min
1% aqueous formic acid is eluted as eluent, and the dosage of the eluent is twice of column volume, and flow velocity is 0.4~
0.6mL/min；The methanol aqueous solution for containing 0.05% formic acid is finally used to be eluted as eluant, eluent, the dosage of the eluant, eluent
Three times column volume, flow velocity are 0.4~0.6mL/min；Collect eluent.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810225828.5A CN108379335A (en) | 2018-03-19 | 2018-03-19 | The weight-reducing purposes of beans taro leaf ethanol extract |
| CN201910209416.7A CN109985083B (en) | 2018-03-19 | 2019-03-19 | Application of Doudou extract in preparing food or medicine for reducing weight, resisting tumor and improving immunity |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810225828.5A CN108379335A (en) | 2018-03-19 | 2018-03-19 | The weight-reducing purposes of beans taro leaf ethanol extract |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113425783A (en) * | 2021-08-13 | 2021-09-24 | 深圳市巨印科技有限公司 | Oral spray for losing weight |
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2018
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113425783A (en) * | 2021-08-13 | 2021-09-24 | 深圳市巨印科技有限公司 | Oral spray for losing weight |
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