CN108606973A - The weight-reducing purposes of beans taro leaf polyose - Google Patents
The weight-reducing purposes of beans taro leaf polyose Download PDFInfo
- Publication number
- CN108606973A CN108606973A CN201810225208.1A CN201810225208A CN108606973A CN 108606973 A CN108606973 A CN 108606973A CN 201810225208 A CN201810225208 A CN 201810225208A CN 108606973 A CN108606973 A CN 108606973A
- Authority
- CN
- China
- Prior art keywords
- beans taro
- taro leaf
- drug
- weight
- food
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 244000205754 Colocasia esculenta Species 0.000 title claims abstract description 108
- 235000006481 Colocasia esculenta Nutrition 0.000 title claims abstract description 108
- 235000010627 Phaseolus vulgaris Nutrition 0.000 title claims abstract description 108
- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 108
- 235000013305 food Nutrition 0.000 claims abstract description 39
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 38
- 239000003814 drug Substances 0.000 claims abstract description 33
- 229940079593 drug Drugs 0.000 claims abstract description 28
- 210000004185 liver Anatomy 0.000 claims abstract description 27
- 235000005911 diet Nutrition 0.000 claims abstract description 21
- 230000037213 diet Effects 0.000 claims abstract description 21
- 150000003626 triacylglycerols Chemical class 0.000 claims abstract description 17
- 210000002966 serum Anatomy 0.000 claims abstract description 16
- 235000009200 high fat diet Nutrition 0.000 claims abstract description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 24
- 235000009508 confectionery Nutrition 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 17
- 150000004676 glycans Chemical class 0.000 claims description 17
- 229920001282 polysaccharide Polymers 0.000 claims description 17
- 239000005017 polysaccharide Substances 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- 239000000706 filtrate Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 claims description 10
- 108010082126 Alanine transaminase Proteins 0.000 claims description 10
- 239000012141 concentrate Substances 0.000 claims description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims description 9
- 239000012045 crude solution Substances 0.000 claims description 9
- 235000019441 ethanol Nutrition 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 6
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000002390 rotary evaporation Methods 0.000 claims description 5
- 210000000577 adipose tissue Anatomy 0.000 claims description 4
- 239000012043 crude product Substances 0.000 claims description 4
- 210000002216 heart Anatomy 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000004090 dissolution Methods 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 230000003946 protein process Effects 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 2
- 238000004821 distillation Methods 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 238000003760 magnetic stirring Methods 0.000 claims description 2
- 239000003610 charcoal Substances 0.000 claims 1
- 208000008589 Obesity Diseases 0.000 abstract description 22
- 235000020824 obesity Nutrition 0.000 abstract description 22
- 241000699670 Mus sp. Species 0.000 abstract description 21
- 230000000694 effects Effects 0.000 abstract description 7
- 208000017667 Chronic Disease Diseases 0.000 abstract description 3
- 238000011156 evaluation Methods 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 19
- 235000012000 cholesterol Nutrition 0.000 description 10
- 238000003304 gavage Methods 0.000 description 8
- 238000011160 research Methods 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- 235000004213 low-fat Nutrition 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 208000035475 disorder Diseases 0.000 description 4
- 230000037356 lipid metabolism Effects 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 210000004279 orbit Anatomy 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000011740 C57BL/6 mouse Methods 0.000 description 3
- 244000062793 Sorghum vulgare Species 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 238000003149 assay kit Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000005802 health problem Effects 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 235000019713 millet Nutrition 0.000 description 3
- 210000000952 spleen Anatomy 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 206010033307 Overweight Diseases 0.000 description 2
- 108090000340 Transaminases Proteins 0.000 description 2
- 229930003427 Vitamin E Natural products 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 210000003486 adipose tissue brown Anatomy 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 102000014898 transaminase activity proteins Human genes 0.000 description 2
- 235000019165 vitamin E Nutrition 0.000 description 2
- 229940046009 vitamin E Drugs 0.000 description 2
- 239000011709 vitamin E Substances 0.000 description 2
- 241001098044 Apios americana Species 0.000 description 1
- 235000003276 Apios tuberosa Nutrition 0.000 description 1
- 241000345998 Calamus manan Species 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 241000522190 Desmodium Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 238000013218 HFD mouse model Methods 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000003579 anti-obesity Effects 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 210000000028 corpus adiposum pararenale Anatomy 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 210000000918 epididymis Anatomy 0.000 description 1
- 201000010063 epididymitis Diseases 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 150000002515 isoflavone derivatives Chemical class 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 210000003739 neck Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 235000012950 rattan cane Nutrition 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Obesity (AREA)
- Polymers & Plastics (AREA)
- General Chemical & Material Sciences (AREA)
- Materials Engineering (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Epidemiology (AREA)
- Child & Adolescent Psychology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of weight-reducing purposes of beans taro leaf polyose, and developing natural diet products for fields such as medicine, Food Sciences provides new resources.Beans taro leaf polyose in the present invention is proved through high fat diet obesity-induced mice experimental evaluation, can significantly reduce obesity mice weight, reduces triglycerides and total cholesterol level in serum and liver, and function and effect are comprehensively and notable.Therefore, beans taro leaf polyose of the invention is applied to prepare diet food or drug, is occurred with treating and preventing fat and its chronic diseases.
Description
Technical field
The present invention relates to field of medicaments, and in particular to a kind of weight-reducing purposes of beans taro leaf polyose.
Background technology
Beans taro (Apios americana Medikus) originates in the Canadian to Fla. of eastern North America
Southern areas, belong to perennial legume (Papilionaceae subfamily).Its edible portion is underground stem tuber, and its protein contains
Amount is higher than other plant stem tuber, is a kind of not only nutrition but also has the crop of economic value, thus often by American Indian as staple food.Beans
Taro domestic successful introduction from 2009, and by gradually in the ground such as Fuyang, Jinhua, Long You, Wenzhou popularizing planting.Research shows that beans taro
Different parts (flower, rattan, leaf, stem tuber) contain abundant free amino acid, soluble protein, polysaccharide, saponin(e, flavones, different Huang
Ketone, vitamin C, vitamin E and mineral matter and other components.Wherein, the content of vitamin E highest of beans taro leaf, while also containing higher
Content polysaccharide, total saposins and isoflavones have great potentiality to be exploited.
With the rapid development of modern social economy, the living standard of the people significantly improves, obesity becomes the whole world and generally flows
Capable health problem.It is reported in March, 2011 according to the World Health Organization and shows that the whole world in 2008 has more than 1,500,000,000 adults and is in
Overweight level, wherein at least 500,000,000 be fat.The year two thousand thirty is expected, there will be problem of obesity for the population in the U.S. 42%.U.S. will be exceeded
The present population of state 30,000,000.Medical treatment cost increase related to this will reach nearly 550,000,000,000 dollars.And it was delivered at home according to 2010
Research report claim, for population of being obese up to 3.25 hundred million people, amplification is more than the U.S., Britain and Australia at present for China.7 years old or less youngsters
Tong Zhongyou nearly 1/5th is overweight, and 7% is Obese children, ratio Yuan Chao European countries.This number will be also possible to for 20 years in future
It doubles.The common complication of disorders of lipid metabolism has fatty liver, hypertension, diabetes, coronary heart disease, cerebral apoplexy etc..Thus
As it can be seen that disorders of lipid metabolism has become the universal health problem in the whole world, prevention and treatment disorders of lipid metabolism has become 21 generation
The important health problem of discipline, how the generation of prevention and treatment disorders of lipid metabolism and its related complication has caused the whole world
The concern of person.Therefore, the researcher of many nutrition and health care food pays special attention in relation to fat and chronic disease hair at present
The raw and research prevented and topic, but the anti-obesity activity of beans taro leaf polyose is rarely reported.
Invention content
It is the exploitation of the fields such as medicine, Food Science present invention aims at a kind of weight-reducing purposes of beans taro leaf polyose is provided
Natural diet products provide new resources.
In order to solve the above technical problems, the present invention provides a kind of weight-reducing purposes of beans taro leaf polyose：
It is used to prepare diet food or drug；
The improvement of weight-reducing purposes as beans taro leaf polyose of the present invention：
The diet food or drug are the food or drug for reducing high fat diet and leading obesogenous weight.
Weight-reducing purposes as beans taro leaf polyose of the present invention is further improved：
The diet food or drug are the food or drug for reducing adipose tissue mass.
Weight-reducing purposes as beans taro leaf polyose of the present invention is further improved：
The diet food or drug be reduce Triglycerides in Serum (TG) and total cholesterol (TC) content food or
Drug.
Weight-reducing purposes as beans taro leaf polyose of the present invention is further improved：
The diet food or drug be reduce in liver the food of triglycerides (TG) and total cholesterol (TC) content or
Drug.
Weight-reducing purposes as beans taro leaf polyose of the present invention is further improved：
The diet food or drug are to reduce glutamic-pyruvic transaminase (ALT) and glutamic-oxalacetic transaminease (AST) content in serum
Food or drug.
Weight-reducing purposes as beans taro leaf polyose of the present invention is further improved：
The diet food or drug are to adjust glutamic-pyruvic transaminase (ALT) and glutamic-oxalacetic transaminease (AST) content in liver
Food or drug.
Weight-reducing purposes as beans taro leaf polyose of the present invention is further improved：
The preparation method of the beans taro leaf polyose includes the following steps：
1., the preparation of beans taro leaf polyose crude product：
1.1, according to 1g：8~12ml (preferably 1g:Solid-liquid ratio 10ml) first carries out after mixing beans taro leaf and distilled water
It extracts (also achieving enzyme deactivation while extraction), is then beaten, then centrifuge (4000r/min centrifuges 30min), supernatant is taken to filter
And collect filtrate；
1.2, it replaces beans taro leaf to repeat extraction twice by step 1.1 filter residue of centrifugation gained, merges filtrate three times, revolving
Concentration obtains concentrate (to original volume 1/4~1/6)；
1.3, it is 85 ethyl alcohol (straight alcohol) to final ethanol concentration to be added in step 1.2 gained concentrate under stirring condition
± 5%, precipitation is collected after 24 ± 2h is stood at 4 ± 1 DEG C, obtains beans taro leaf Thick many candies；
2., the purifying of beans taro leaf Thick many candies, follow the steps below successively：
A, activated carbon decolorizing：
Beans taro leaf Thick many candies are distilled water dissolution, and (dosage of distilled water need to only can guarantee the dissolving of beans taro leaf Thick many candies i.e.
Can), beans taro leaf Thick many candies solution is obtained, is filtered after activated carbon stirring is added in beans taro leaf Thick many candies solution；
Note：It is above-mentioned in beans taro leaf Thick many candies solution be added activated carbon stirring after filtering the specific steps are：Beans taro leaf is slightly more
It is added after activated carbon stirs 45 ± 5min in 80 ± 10 DEG C of water-baths and filters in sugar juice；Beans taro leaf Thick many candies and activated carbon
Mass ratio is 10~20:1；Above-mentioned beans taro leaf Thick many candies solution, which is substituted, with the filtrate of gained repeats above-mentioned addition activated carbon and stirring
The decolorization filtered afterwards, until the filtrate of final gained is in colourless, polysaccharide crude solution after must decolourizing；
B, Sevage methods take off albumen：
Sevage reagents are added in polysaccharide crude solution after decoloration, 1000 ± 100 turns/min turns in magnetic stirring apparatus
Speed 30 ± 5min of stirring, is then centrifuged for, removes the water layer positioned at lower layer and the albuminate between the solvent layer on upper layer；Institute
Sevage reagents are stated by n-butanol：Chloroform=1:4~6 volume ratio is obtained by mixing；
Polysaccharide crude solution repeats above-mentioned addition after substituting above-mentioned decoloration with the gains after the removing albuminate of gained
Sevage reagents, magnetic agitation, centrifugation remove the de- protein process 5~8 times of albuminate, and the polysaccharide that must remove protein is molten
Liquid；
The polysaccharide solution concentrated by rotary evaporation of protein will be removed until for the 8~12% of original volume, by concentrate in -70~-
90 DEG C of 5~7h of pre-freeze, are then dried to vacuum freeze drier powdered, obtain beans taro leaf polyose.
The invention discloses a kind of preparation method of beans taro leaf polyose and its weight-reducing purposes, are the necks such as medicine, Food Science
Domain develops natural diet products and provides new resources.Beans taro leaf polyose in the present invention is commented through the experiment of high fat diet obesity-induced mice
Valence proves, can significantly reduce obesity mice weight, reduces triglycerides and total cholesterol level in serum and liver, effect effect
Fruit is comprehensively and notable.Therefore, beans taro leaf polyose of the invention is applied to prepare diet food or drug, to treat and prevent obesity
And its chronic diseases occur.The usage of the beans taro leaf polyose of the present invention is oral, dosage about 150~250mg every time, daily
Three times.
The present invention compared with the existing technology, has the following advantages：
1. present invention firstly discovers that beans taro leaf polyose can alleviate the increase of high fat diet obesity-induced mice weight, fertilizer is reduced
Triglycerides and total cholesterol level in fat mice serum and liver show significant Weight-reducing and lipid-lowering effect, have good
Development prospect.
2. the present invention provides new medical application for beans taro leaf polyose, a new application field has been expanded.
Description of the drawings
Fig. 1 is influence of the various concentration beans taro leaf polyose to mouse weight in the present invention.
Fig. 2 is influence of the various concentration beans taro leaf polyose to glutamic-pyruvic transaminase in mouse liver (ALT) content in the present invention.
Fig. 3 is influence of the various concentration beans taro leaf polyose to glutamic-oxalacetic transaminease in mouse liver (AST) content in the present invention.
Fig. 4 is influence of the various concentration beans taro leaf polyose to triglycerides in mouse liver (TG) content in the present invention.
Fig. 5 is influence of the various concentration beans taro leaf polyose to total cholesterol in mouse liver (TC) content in the present invention.
Note：Fig. 1~Fig. 5 of the present invention, in 1~table of table 2：
LFD represents low fat control group (low fat feed of the feeding containing 10% fat)；
HFD represents control group high in fat (high lipid food of the feeding containing 45% fat)；
HFD+L represents low dose group, and (high lipid food+gavage 200mg/ (kgd) the beans taro leaf of feeding containing 45% fat is more
Sugar)；
HFD+H represents high dose group, and (high lipid food+gavage 400mg/ (kgd) the beans taro leaf of feeding containing 45% fat is more
Sugar).
Specific implementation mode
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
There is significant weight-reducing to drop for the weight-reducing purposes of embodiment 1, beans taro leaf polyose, as Figure 1-Figure 5, beans taro leaf polyose
Fat effect.The specific preparation process of beans taro leaf polyose is as follows：
(1), the preparation of beans taro leaf polyose crude product：
(1.1), according to 1g:The solid-liquid ratio of 10ml weighs beans taro leaf 100g and is added 1000mL distilled water, 80 DEG C of water-bath 4h into
Row extraction (enzyme deactivation is also achieved while extraction), is then beaten, then centrifuges (4000r/min centrifuges 30min), by centrifugation gained
Supernatant filter and collect filtrate；
(1.2), the filter residue of step (1.1) centrifugation gained is replaced into beans taro leaf in step (1.1), repeats extraction 2 times, merges
The concentration of filtrate rotary evaporation obtains concentrate until for the 1/5 of original volume three times.
(1.3), ethyl alcohol (straight alcohol) is added to final ethanol concentration in concentrate obtained by step (1.2) under stirring condition
It is 85%, is stood at 4 DEG C and collect precipitation afterwards for 24 hours, obtain beans taro leaf Thick many candies；
(2), the purifying of beans taro leaf polyose crude product, follows the steps below successively：
A, activated carbon decolorizing：
By beans taro leaf Thick many candies be placed in beaker with distillation water dissolution (dosage of distilled water need to only can guarantee that beans taro leaf is slightly more
Sugar dissolving), beans taro leaf Thick many candies solution is obtained, activated carbon is added in beans taro leaf Thick many candies solution in 80 ± 10 DEG C of water-baths
It is filtered after middle stirring 45min；The mass ratio of beans taro leaf Thick many candies and activated carbon is 15:1；
It is substituted after above-mentioned beans taro leaf Thick many candies solution repeats above-mentioned addition activated carbon and stirring and is filtered with the filtrate of gained
Decolorization 3 times, the filtrate of final gained is in colourless, polysaccharide crude solution after must decolourizing at this time；
B, Sevage methods take off albumen：
According to 4:100mL Sevage reagents, Yu Ci is added in polysaccharide crude solution after 400mL decolorations in 1 volume ratio
1000 turns/min rotating speeds stir 30min in power blender, are then centrifuged for, and water layer and position positioned at lower layer are removed by separatory funnel
Albuminate between the solvent layer on upper layer；The Sevage reagents are by n-butanol：Chloroform=1：5 volume ratio mixing and
；
Polysaccharide crude solution repeats above-mentioned addition after substituting above-mentioned decoloration with the gains after the removing albuminate of gained
Sevage reagents, acutely oscillation, centrifugation, remove the de- protein process 5 times of albuminate, must remove the polysaccharide solution of protein；
The polysaccharide solution concentrated by rotary evaporation for removing protein is obtained into concentrate (about the 10% of original volume), by concentrate
In -80 DEG C of pre-freeze 6h, then use vacuum freeze drier 48h (technological parameter that vacuum freeze drier is set as -40 DEG C,
It 1.2Pa) is dried into powdered (moisture content≤0.1%), obtains beans taro leaf polyose.
It is small that the beans taro leaf polyose prepared in aforementioned manners is intervened the C57BL/6 obesities induced by high fat diet
Mouse observes its changes of weight and detects triglyceride and cholesterol level variation in serum and liver, the specific steps are：
1, experimental animal grouping and processing；
50 male C57BL/6 mouse are put into animal house and feed, and feeding environment is 12h alternation of light and darkness, and temperature is 23 ± 3
DEG C, relative humidity 50%.After adaptable fed 1 week, mouse is randomly divided into 5 groups, every group 10.
Group designs：Low fat control group (LFD), low fat feed of the feeding containing 10% fat；Control group high in fat
(HFD), high lipid food of the feeding containing 45% fat；Low dosage beans taro leaf polyose group (HFD+L), feeding are high in fat containing 45% fat
Feed and gavage 200mg/ (kgd) beans taro leaf polyose；High dose beans taro leaf polyose group HFD+H), height of the feeding containing 45% fat
Fat feed and gavage 400mg/ (kgd) beans taro leaf polyose.Mouse ad lib and drinking-water during experiment, Therapy lasted 12 weeks.
2, influence of the record beans taro leaf polyose to obesity mice weight：
Weight and food ration are recorded during experiment weekly.According to Fig. 1 it can be found that beans taro leaf polyose intervene 12 weeks after, together
Control group high in fat is compared, and low dose group and high dose group can alleviate the C57BL/6 mouse weights that high fat diet induces and increase.
The influence of embodiment 2, beans taro leaf polyose to organ indexs such as obesity mice fat, livers；
For 24 hours by the mouse fasting after gavage in embodiment 1 12 weeks, it weighs in, eye socket takes mouse cervical dislocation after blood
It puts to death, takes kidney week and epididymis peripheral adipose, quickly win the heart, liver,spleen,kidney, brown fat etc. and organize and record weight, specific number
According to as shown in table 1.
Influence of the 1 beans taro leaf polyose intervention of table to mice organs index
According to table 1 it is found that the obesity mice liver index that beans taro leaf polyose intervention can alleviate high fat diet induction declines,
Various dose beans taro leaf polyose intervention simultaneously can significantly reduce all fat of testis in obesity mice body and perirenal fat index, high
Dosage beans taro leaf polyose intervention is remarkably improved obesity mice index and spleen index, the beans taro leaf polyose intervention pair compared with control group high in fat
Liver, brown fat, heart, kidney, pancreas index do not make significant difference.The above result shows that beans taro leaf polyose can significantly change
The variation of kind high fat diet obesity-induced mice body fat index and index and spleen index.
Embodiment 3, beans taro leaf polyose turn obesity mice serum triglyceride, total cholesterol, glutamic-pyruvic transaminase and millet straw
The influence of ammonia enzyme content；
For 24 hours by the mouse fasting after gavage in embodiment 1 12 weeks, eye socket puts to death mouse cervical dislocation after taking blood, blood
Sample 1500 leaves heart 15min, takes serum keeping and using Toshiba's automatic clinical chemistry analyzer to Triglycerides in Serum and always
Cholesterol level is measured, and specific data are as shown in table 2.
Influence of the 2 beans taro leaf polyose intervention of table to mice serum index
According to table 2 it is found that the beans taro leaf polyose intervention of high dose can reduce the obesity mice serum of high fat diet induction
Middle total cholesterol level, while low dose group can significantly reduce obesity mice Triglycerides in Serum, total cholesterol contains
Amount.Just two dosage groups can significantly reduce the content of glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease in obesity mice serum.With
Above the result shows that beans taro leaf polyose can improve high fat diet obesity-induced mice Triglycerides in Serum, total cholesterol, paddy third
The variation of transaminase and glutamic-oxalacetic transaminease content.
The influence of embodiment 4, beans taro leaf polyose to obesity mice liver glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease content；
For 24 hours by the mouse fasting after gavage in embodiment 1 12 weeks, it weighs in, eye socket takes mouse cervical dislocation after blood
It puts to death, mouse liver is taken to turn ammonia according to glutamic-pyruvic transaminase assay kit (Bioengineering Research Institute is built up in Nanjing) and millet straw
Enzyme assay kit (Bioengineering Research Institute is built up in Nanjing) specification is measured.
According to fig. 2 with Fig. 3 it is found that glutamic-pyruvic transaminase and millet straw turn ammonia in high in fat group of mouse liver compared with low fat control group
The content of enzyme dramatically increases, and can be significantly improved in the liver as caused by high fat diet after the beans taro leaf polyose intervention of high dose
The increase of transaminase content, the above result shows that beans taro leaf polyose has significant protect liver effect.
The influence of embodiment 5, beans taro leaf polyose to obesity mice liver tg and total cholesterol level；
For 24 hours by the mouse fasting after gavage in embodiment 1 12 weeks, it weighs in, eye socket takes mouse cervical dislocation after blood
It puts to death, mouse liver is taken to contain according to triglyceride assay kit (Bioengineering Research Institute is built up in Nanjing) and total cholesterol
Amount assay kit (Bioengineering Research Institute is built up in Nanjing) specification is measured.
According to Fig. 4 with Fig. 5 it is found that triglycerides and total cholesterol contain in high in fat group of mouse liver compared with low fat control group
Amount dramatically increases, and can be significantly improved after the beans taro leaf polyose intervention of various dose total in the liver as caused by high fat diet
The increase of cholesterol can significantly reduce the accumulation of triglycerides in liver after the beans taro leaf polyose intervention of high dose.The above knot
Fruit shows that beans taro leaf polyose can effectively alleviate the accumulation of triglycerides and total cholesterol in liver.
To sum up the present invention proposes a kind of weight-reducing purposes of beans taro leaf polyose, is that the exploitation of the fields such as medicine, Food Science is natural
Diet products provide new resources.It is related to application of the beans taro leaf polyose in preparing diet products simultaneously.Listed above is only this
Several examples of the implementation of invention.It is clear that the invention is not restricted to above example, acceptable there are many deformations.This field it is general
All deformations that logical technical staff directly can export or associate from present disclosure are considered as the guarantor of the present invention
Protect range.
Claims (8)
1. the weight-reducing purposes of beans taro leaf polyose, it is characterised in that：
It is used to prepare diet food or drug.
2. the weight-reducing purposes of beans taro leaf polyose according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing high fat diet and leading obesogenous weight.
3. the weight-reducing purposes of beans taro leaf polyose according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing adipose tissue mass.
4. the weight-reducing purposes of beans taro leaf polyose according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing Triglycerides in Serum and total cholesterol level.
5. the weight-reducing purposes of beans taro leaf polyose according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing triglycerides and total cholesterol level in liver.
6. the weight-reducing purposes of beans taro leaf polyose according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for reducing glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease content in serum.
7. the weight-reducing purposes of beans taro leaf polyose according to claim 1, it is characterised in that：
The diet food or drug are the food or drug for adjusting glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease content in liver.
8. according to the weight-reducing purposes of any beans taro leaf polyoses of claim 1-7, it is characterised in that：
The preparation method of the beans taro leaf polyose includes the following steps：
1., the preparation of beans taro leaf polyose crude product：
1.1, according to 1g：The solid-liquid ratio of 8~12ml first extracts after mixing beans taro leaf and distilled water, is then beaten, then from
The heart takes supernatant to filter and collects filtrate；
1.2, it replaces beans taro leaf to repeat extraction twice by step 1.1 filter residue of centrifugation gained, merges filtrate three times, concentrated by rotary evaporation
Obtain concentrate；
1.3, it is 85 ± 5%, 4 ± 1 DEG C ethyl alcohol to final ethanol concentration to be added in step 1.2 gained concentrate under stirring condition
Precipitation is collected after 24 ± 2h of lower standing, obtains beans taro leaf Thick many candies；
2., the purifying of beans taro leaf Thick many candies, follow the steps below successively：
A, activated carbon decolorizing：
By the distillation water dissolution of beans taro leaf Thick many candies, beans taro leaf Thick many candies solution is obtained, is added and lives in beans taro leaf Thick many candies solution
Property charcoal stirring after filter；
Above-mentioned beans taro leaf Thick many candies solution, which is substituted, with the filtrate of gained repeats the decoloration filtered after above-mentioned addition activated carbon and stirring
Process, until the filtrate of final gained is in colourless, polysaccharide crude solution after must decolourizing；
B, Sevage methods take off albumen：
Sevage reagents are added in polysaccharide crude solution after decoloration, 1000 ± 100 turns/min rotating speeds stir in magnetic stirring apparatus
30 ± 5min is mixed, is then centrifuged for, the water layer positioned at lower layer and the albuminate between the solvent layer on upper layer are removed；It is described
Sevage reagents are by n-butanol：Chloroform=1:4~6 volume ratio is obtained by mixing；
Polysaccharide crude solution repeats above-mentioned addition after substituting above-mentioned decoloration with the gains after the removing albuminate of gained
Sevage reagents, magnetic agitation, centrifugation remove the de- protein process 5~8 times of albuminate, and the polysaccharide that must remove protein is molten
Liquid；
The polysaccharide solution concentrated by rotary evaporation of protein will be removed until for the 8~12% of original volume, by concentrate in -70~-90 DEG C
5~7h of pre-freeze, is then dried to vacuum freeze drier powdered, obtains beans taro leaf polyose.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810225208.1A CN108606973A (en) | 2018-03-19 | 2018-03-19 | The weight-reducing purposes of beans taro leaf polyose |
| CN201910209416.7A CN109985083B (en) | 2018-03-19 | 2019-03-19 | Application of Doudou extract in preparing food or medicine for reducing weight, resisting tumor and improving immunity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810225208.1A CN108606973A (en) | 2018-03-19 | 2018-03-19 | The weight-reducing purposes of beans taro leaf polyose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108606973A true CN108606973A (en) | 2018-10-02 |
Family
ID=63659187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810225208.1A Withdrawn CN108606973A (en) | 2018-03-19 | 2018-03-19 | The weight-reducing purposes of beans taro leaf polyose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108606973A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113425783A (en) * | 2021-08-13 | 2021-09-24 | 深圳市巨印科技有限公司 | Oral spray for losing weight |
-
2018
- 2018-03-19 CN CN201810225208.1A patent/CN108606973A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113425783A (en) * | 2021-08-13 | 2021-09-24 | 深圳市巨印科技有限公司 | Oral spray for losing weight |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105983016B (en) | A pharmaceutical composition containing silybin | |
| CN101283717B (en) | Flower silk leaf bagged tea and its preparation method | |
| CN108606973A (en) | The weight-reducing purposes of beans taro leaf polyose | |
| CN112741851A (en) | Method for extracting sparrow tea extract by eutectic solvent method and preparation and application of granules | |
| CN102524708B (en) | Yuge powder with functions of weight loss and beauty and preparation method for Yuge powder | |
| KR102160672B1 (en) | Composition for Anti-diabetic comprising Hydrorized Extract of Glub | |
| CN104945532B (en) | The preparation method of Gynura divaricata polysaccharide | |
| CN101167781A (en) | Orally-administered hypoglycemic sweet potato leaf single prescription traditional Chinese medicine and preparation method thereof | |
| CN105713942A (en) | Broccoli polypeptide active ingredient, and preparation method and application thereof | |
| CN105943617A (en) | Application of whole hemp extract in preparation of anti-hepatitis drugs | |
| CN107551167B (en) | Application of spartina alterniflora extract in preparation of functional product for reducing blood uric acid | |
| CN105079532A (en) | Healthcare green tea capable of reducing blood fat and loosing weight and preparation method of healthcare green tea | |
| CN108379335A (en) | The weight-reducing purposes of beans taro leaf ethanol extract | |
| CN108392513A (en) | The weight-reducing purposes of beans taro ethanol extract | |
| CN108272817A (en) | The weight-reducing purposes of beans taro polysaccharide | |
| CN108371673A (en) | The weight-reducing purposes of beans taro stem tuber ethanol extract | |
| CN107412393B (en) | Preparation method and application of clausena lansium leaf polyphenol extract | |
| CN110624006A (en) | A health wine containing effective components of fructus Quzhazhii | |
| CN1231224C (en) | Sealwort polysaccharide konjaku glucomannan capsule | |
| CN109985083A (en) | Application of the beans taro extract in preparation weight-reducing, food or drug antitumor, that improve immunity | |
| KR100391195B1 (en) | a manufacturing technique of a beverage using of an educt which is distilled from Liriope spicata Lour | |
| CN110279758A (en) | A kind of tree peony leaf dried powder and preparation method thereof and the application as xanthine oxidase inhibitor | |
| CN107095979A (en) | A kind of Chinese medicine composition, its preparation method and application for being applied to treatment hyperlipemia | |
| CN109021135A (en) | A kind of codonopsis pilosula var. modesta polysaccharide formulation and preparation method thereof | |
| CN104288220A (en) | Sophora japonica bud extract rich in inositol and rutin, and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181002 |